MC11g_new0040 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0040 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.9e-106
Identity = 214/521 (41.07%), Postives = 296/521 (56.81%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIF----FLYA-MFKIYGRFDANRKRLPPEAGGGLPVIGHLH 60
           MEL  H  ++    G+++ IF    FLY  + K++G       +  P  GG  P+ GHL 
Sbjct: 1   MELVLHFLNT-TTIGVVSLIFLLCLFLYGPLKKVHG-----SSKEAPTVGGAWPIFGHLP 60

Query: 61  LLAGKEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVA 120
           LL G +  H     +A+  GP+FT++ G    L+VS+WE+ARECFTTND   ++RPKL+ 
Sbjct: 61  LLIGSKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLV 120

Query: 121 SNLLGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCV 180
           + L+ Y+ AML + PYGP+WR +RKI   ++L++ R+EQLQ +R+SEVQ+SI +LY++  
Sbjct: 121 AELMCYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWR 180

Query: 181 GR--EGQKAPVEMKTWFGDVTLNTIFRIVVGKRF--STAVDGGGDEEYRKALRDFFEWFG 240
            +  E   A VE+K WF     N + R+VVGKRF  +TA D    E+  KA+ +F    G
Sbjct: 181 SQKNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATDEKA-EKCVKAVDEFMRLAG 240

Query: 241 MFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDV 300
           +F   DA P+L WLD GG++KAMK+TAK LD +  +WL+EHRQKR  G+ +    DFM+V
Sbjct: 241 VFTVGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNV 300

Query: 301 MVSDVRDGNNGEFSRYDADTVTKAT--------------------CLV------------ 360
           M+S + DG        DADT+ K+T                    CL+            
Sbjct: 301 MLSSL-DGKT--IDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILENKAE 360

Query: 361 -----------------------------------SPLAVPHEAVEDCTVAGYHIPAGTR 420
                                               PL+ P E  EDCT+ GYH+  GTR
Sbjct: 361 LDIQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTR 420

Query: 421 LIVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQ 446
           LI N+ K+  DP VW DP EF+P+RFLT+ KD DV+G   QL+PFG+GRR+CPGISF LQ
Sbjct: 421 LITNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQ 480

BLAST of MC11g_new0040 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.3e-105
Identity = 207/504 (41.07%), Postives = 278/504 (55.16%), Query Frame = 0

Query: 16  IIAGIFFLYAMFKIYGRFDANRK-RLPPEAGGGLPVIGHLHLLAGKEPAHLTFAKMADAF 75
           ++ G F L   F  +     N K + PP+  G  P+IGHLHLLA  +  H   +  AD  
Sbjct: 10  LLLGFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKN 69

Query: 76  GPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPH 135
           GP+F ++ GV   L+V+N EIA+ECFTTNDR F +RP  VA+ ++GY++ MLG+ PYGP+
Sbjct: 70  GPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPY 129

Query: 136 WRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQKAPVEMKTWFGDVT 195
           WR +RKI  L+ L+N RL+ L+H+  SE+  S K+LY+L   +      V+MK W  D+T
Sbjct: 130 WRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMKQWLADLT 189

Query: 196 LNTIFRIVVGKRFSTAVDGGGDEEYR-----KALRDFFEWFGMFVPSDAFPFLSWLDLGG 255
           LN   ++VVGKRF  +      EE       K LR+ F   G FV SD  P+L WLDLGG
Sbjct: 190 LNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGG 249

Query: 256 HQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYDA 315
           H+K MK+T K LD +F  WL EH++KR SG    +D DFMDVM+S + +   G     D 
Sbjct: 250 HEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEESKLGN----DV 309

Query: 316 DTVTKATCLV-------------------------------------------------- 375
           DT+ K  CL                                                   
Sbjct: 310 DTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLV 369

Query: 376 ------------------SPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWED 435
                              PL  P    EDCT+AGYH+ AGTRLIVN  K+QRDP VW  
Sbjct: 370 KLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQ 429

Query: 436 PCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLHGYEINR 446
           P E++PERFL  ++D D++GQ  +LIPFG+GRR CP IS ALQV+ LTLA++LHG+E+  
Sbjct: 430 PHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRT 489

BLAST of MC11g_new0040 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.0e-105
Identity = 199/517 (38.49%), Postives = 289/517 (55.90%), Query Frame = 0

Query: 3   LNSHVTSSPAAAGIIAGIFFLYAMFKIYGRFDANRKRLPPEAGGGLPVIGHLHLLAGKEP 62
           LN    ++ A A I++ IF    +++       +R +  P   G  P++GHL LL G + 
Sbjct: 5   LNCLNLNTIAIASILSLIFLCLFLYR-----KNSRGKDAPVVSGAWPILGHLSLLNGSQT 64

Query: 63  AHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYD 122
            H     +AD +GP+FT++ G+   L++SNWE+++E FTTND   +SRPKLVA  ++ Y+
Sbjct: 65  PHKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYN 124

Query: 123 FAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVG---REG 182
            A +GL PYGP+WR +RKI T + L+N R+EQ  HIR+SEV++SIK+L+++       E 
Sbjct: 125 QAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNES 184

Query: 183 QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEE---YRKALRDFFEWFGMFVPS 242
           +   V++K W   +T N + R+VVGKR+   +   G ++   + K +R+F    G F  +
Sbjct: 185 RYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVA 244

Query: 243 DAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDV 302
           D  P L WLDLGGH+KAMK  AK +D++  +WL+EHRQK+  G+ +  D DFMDVM+S +
Sbjct: 245 DGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL 304

Query: 303 RDGNNGEFSRYDADTVTKATCL-------------------------------------- 362
              N  +   +DADT+ KAT L                                      
Sbjct: 305 ---NGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQ 364

Query: 363 ------------------------------VSPLAVPHEAVEDCTVAGYHIPAGTRLIVN 422
                                          +P + P E  E+C + GYHI  GTRLI N
Sbjct: 365 IGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHN 424

Query: 423 LQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHL 446
           L K+ RDP VW DP EF+PERFLT+ KD D+RG + +L+PFG+GRR+C G+S  L ++H 
Sbjct: 425 LWKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHF 484

BLAST of MC11g_new0040 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 370.9 bits (951), Expect = 2.0e-101
Identity = 202/537 (37.62%), Postives = 285/537 (53.07%), Query Frame = 0

Query: 3   LNSHVTSSPAAAGIIAGIFFLYAMFKIYGRFDANRKRLPPEAGGGLPVIGHLHLLAGKEP 62
           LN   T++ A   +I+ +FFL+   K+      +  + PP   G  P++GHL L+   + 
Sbjct: 3   LNYLNTTTIAFISLISLLFFLFRFSKV------SHTKEPPIISGSWPLLGHLPLMRNTQT 62

Query: 63  AHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYD 122
            H T   + D +GPIFT++ G +  L++SNWE+A+ECFT ND + +SRPK VA  L+ Y+
Sbjct: 63  PHKTLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYN 122

Query: 123 FAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQK- 182
            A +G  PYG +WR +RKI TL++L+N R+E L HIR+SEVQ+SIK+L  +   +   + 
Sbjct: 123 QAFIGWAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQY 182

Query: 183 --------------------APVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRK 242
                                 VE+K WF  +TLN + R+VVGKR    VD    EE ++
Sbjct: 183 GLLDDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKR 242

Query: 243 AL---RDFFEWFGMFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNS 302
            L   RDF    G F   D  PFL WLDLGGH+K MKK AK  D + ++WL+EHR+K+  
Sbjct: 243 FLENIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGL 302

Query: 303 G--QVLMEDHDFMDVMVSDVRDGNNGEFSRYDADTVTKAT-------------------- 362
           G    ++ + DFMD M+  ++D        +D DT+ KAT                    
Sbjct: 303 GSEDKVVGERDFMDAMLLVLKD---KPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAM 362

Query: 363 CLV------------------------------------------------SPLAVPHEA 422
           CL+                                                +P + P E 
Sbjct: 363 CLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREF 422

Query: 423 VEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIP 446
            EDCT+ GYHI  GTRL+ NL K+ RDP VW DP EF+PERFL++ KD DVRGQ+ +L+P
Sbjct: 423 TEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLP 482

BLAST of MC11g_new0040 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.7e-100
Identity = 196/484 (40.50%), Postives = 272/484 (56.20%), Query Frame = 0

Query: 42  PEAGGGLPVIGHLHLLAGKEP-AHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECF 101
           P   G  P+IGHLHLL GKE   + T  KMAD +GP  +++ G +   +VS++E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 102 TTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRI 161
           T ND+  ASRP   A+  +GY+FA+ G  PY   WR +RKIAT++LL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 162 SEVQSSIKKLYELCVGREGQK-APVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGD--- 221
           SE+   +K LY L     G K   V++K+W  D+TLN I R+V GKR+     GGG    
Sbjct: 153 SEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSS 212

Query: 222 ------EEYRKALRDFFEWFGMFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLK 281
                  + +KA+  FF   G+F  SDAFP LS+ DL GH+K MK+T   LD + ++W++
Sbjct: 213 EDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIE 272

Query: 282 EHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYDADTVTKATCL------------ 341
            HRQ+R        D DF+DVM+S    G      +YDA+T  K+TCL            
Sbjct: 273 NHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHL-QYDANTSIKSTCLALILGGSDTSAS 332

Query: 342 --------------------------------------------------------VSPL 401
                                                                     PL
Sbjct: 333 TLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPL 392

Query: 402 AVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWEDPCEFRPERFLTSQ-KDFDVRG 446
             P EA+EDCTVAGY++P GTRLIVN+ K+QRDP+V+ +P EFRPERF+T + K+FDVRG
Sbjct: 393 LGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRG 452

BLAST of MC11g_new0040 vs. NCBI nr
Match: XP_023535456.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677 bits (1747), Expect = 1.10e-240
Identity = 345/517 (66.73%), Postives = 393/517 (76.02%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIYG-RFDANRKRLPPEAGGGLPVIGHLHLLAG 60
           MEL+   +SS   AGI A I FLYA+ KIYG  F A+RKRLPPEAGG LP+IGHLH LAG
Sbjct: 1   MELSCF-SSSDVFAGIFALIIFLYALSKIYGGTFGAHRKRLPPEAGGALPLIGHLHRLAG 60

Query: 61  KEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLL 120
            EPAH+ FAKMADA+GPIFTVR GV TNLIVSNWEIARECF+TNDRIFASRPKLVAS LL
Sbjct: 61  NEPAHIAFAKMADAYGPIFTVRLGVYTNLIVSNWEIARECFSTNDRIFASRPKLVASKLL 120

Query: 121 GYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCV-GRE 180
           GYD+AM+GL PYGPHWRHVRK+ATL+LLTN RLEQLQHIR+ EV+SSIKKLYE+CV GR 
Sbjct: 121 GYDYAMMGLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRS 180

Query: 181 G---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRKALRDFFEWFGMFVP 240
               +   VEMKTWFGD+TLNTIFRIVVGKRFSTAV+G G+EEYR ALRDFFEWFG+FVP
Sbjct: 181 NGSNEGVLVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVP 240

Query: 241 SDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMED-HDFMDVMVS 300
           SD+FPFL WLDLGGH+KAMK TAKVLDEVFDKWL+EHRQ +N  +  ME+ H FMDVM+S
Sbjct: 241 SDSFPFLKWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQNKNVSEGKMEEPHHFMDVMLS 300

Query: 301 DVRDGNNGEFSRYDADTVTKATCLV----------------------------------- 360
           +V+D  +G+ S YDA TVTKATCL                                    
Sbjct: 301 NVKD--DGQLSNYDAHTVTKATCLALILAGSDTTTITMVWALSLLLNHPEVIERAQLELD 360

Query: 361 ---------------------------------SPLAVPHEAVEDCTVAGYHIPAGTRLI 420
                                            +P+ +PHE++EDCTV GYHIPAGTRLI
Sbjct: 361 EHVGRQRQVRESDVKNLLYLQAIVKETLRLYPAAPILIPHESIEDCTVVGYHIPAGTRLI 420

Query: 421 VNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVM 443
           VN+QKLQRDPQ+W+DPCEFRPERFLT  KDFDVRGQSPQLIPFG+GRR+CPGISFALQV+
Sbjct: 421 VNVQKLQRDPQIWKDPCEFRPERFLTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQVL 480

BLAST of MC11g_new0040 vs. NCBI nr
Match: XP_022935739.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 673 bits (1736), Expect = 5.38e-239
Identity = 345/518 (66.60%), Postives = 396/518 (76.45%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIYG-RFDANRKRLPPEAGGGLPVIGHLHLLAG 60
           MEL+   +SS   AGI+A I FLY++ KIYG  F A+R RLPPEAGG LP+IGHLH LAG
Sbjct: 1   MELSCF-SSSDVFAGILALIIFLYSLSKIYGGTFGAHRMRLPPEAGGALPLIGHLHRLAG 60

Query: 61  KEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLL 120
            EPAH++FAKMADA+GPIFTVR GV TNLIVSNWEIARECF+T DRIFASRPKLVAS LL
Sbjct: 61  NEPAHISFAKMADAYGPIFTVRLGVYTNLIVSNWEIARECFSTKDRIFASRPKLVASKLL 120

Query: 121 GYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCV-GRE 180
           GYD+AM+GL PYGPHWRHVRK+ATL+LLTN RLEQLQHIR+ EV+SSIKKLYE+CV GR 
Sbjct: 121 GYDYAMMGLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRN 180

Query: 181 G---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRKALRDFFEWFGMFVP 240
               +   VEMKTWFGD+TLNTIFRIVVGKRFSTAV+G G+EEYR ALRDFFEWFG+FVP
Sbjct: 181 NGSNEGVLVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVP 240

Query: 241 SDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKR-NSGQVLMEDH-DFMDVMV 300
           SD+FPFL WLDLGGH+KAMK TAKVLDEVFDKWL+EHRQK+ N G+  MED  DFMDVM+
Sbjct: 241 SDSFPFLKWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVML 300

Query: 301 SDVRDGNNGEFSRYDADTVTKATCLV---------------------------------- 360
           S+V+D  +G+ S YDA TVTKATCL                                   
Sbjct: 301 SNVKD--DGQLSNYDAHTVTKATCLALILAGSDTTTITMVWALSLLLNHPEVIERAQLEL 360

Query: 361 ----------------------------------SPLAVPHEAVEDCTVAGYHIPAGTRL 420
                                             +P+ +PHE++EDCTVAGYHIPAGTRL
Sbjct: 361 DEQVGRQRQVREFDVKNLLYLQAIVKETLRLYPAAPILIPHESIEDCTVAGYHIPAGTRL 420

Query: 421 IVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQV 443
           IVN+QKLQRDPQ+W+DPCEFRPERFLT  KDFDVRGQSPQLIPFG+GRR+CPGISFALQV
Sbjct: 421 IVNVQKLQRDPQIWKDPCEFRPERFLTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQV 480

BLAST of MC11g_new0040 vs. NCBI nr
Match: XP_038897198.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 671 bits (1731), Expect = 3.21e-238
Identity = 342/521 (65.64%), Postives = 396/521 (76.01%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIYG-RFDANRKRLPPEAGGGLPVIGHLHLLAG 60
           MEL+S  +     AGI A I FLYA+ KIYG R   +RKRLPPEA G LP+IGHLHLL  
Sbjct: 1   MELSSF-SFPDTVAGIFAVIIFLYALLKIYGGRVRPHRKRLPPEASGALPLIGHLHLLDA 60

Query: 61  KEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLL 120
            EPAH TFAKMADA+GPIFT+R G  TNLIVSNW+IARECFTTNDRIFASRPKLVAS LL
Sbjct: 61  NEPAHTTFAKMADAYGPIFTLRLGAYTNLIVSNWKIARECFTTNDRIFASRPKLVASKLL 120

Query: 121 GYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREG 180
           GYD+AMLGL PYGPHWRHVRKIA L+LLTNHRLE+LQHIR+SEVQ+S+KKLYELCV  + 
Sbjct: 121 GYDYAMLGLSPYGPHWRHVRKIAMLELLTNHRLEKLQHIRVSEVQTSMKKLYELCVRNKD 180

Query: 181 ---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGD--EEYRKALRDFFEWFGMFV 240
              +K  VEMKTWFGD+TLNTIFRIVVGKRFSTAVD  G+  EEYRKALRDFFEWFG+FV
Sbjct: 181 NSEKKTLVEMKTWFGDITLNTIFRIVVGKRFSTAVDASGNDNEEYRKALRDFFEWFGVFV 240

Query: 241 PSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNS-GQVLMEDHDFMDVMV 300
           PSD+FPFL WLDLGGH+KAMKKTAK+LDEVFDKWL+EH+Q RN+ G+  +E+HDFMDVM+
Sbjct: 241 PSDSFPFLKWLDLGGHEKAMKKTAKMLDEVFDKWLQEHQQNRNNIGEENVEEHDFMDVML 300

Query: 301 SDVRDGNNGEFSRYDADTVTKATCLV---------------------------------- 360
           S V+D  +G+ S+YDADTVTKATCL                                   
Sbjct: 301 SRVKD--DGQLSKYDADTVTKATCLALILAGSDTTTVTMIWALSLLLNNPEVLKRAQLEL 360

Query: 361 ----------------------------------SPLAVPHEAVEDCTVAGYHIPAGTRL 420
                                             +P+ +PHE++EDC VAGYHIP GTRL
Sbjct: 361 DEHVGRQRQVRESDVNNLLYLQAIVKETLRLYPAAPILIPHESIEDCMVAGYHIPKGTRL 420

Query: 421 IVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQV 445
           IVN+QKLQ+DP++W+DPCEFRPERFLTSQKDFDVRGQSPQLIPFG+GRR+CPGI FA+QV
Sbjct: 421 IVNIQKLQKDPEIWKDPCEFRPERFLTSQKDFDVRGQSPQLIPFGSGRRMCPGILFAMQV 480

BLAST of MC11g_new0040 vs. NCBI nr
Match: XP_004147803.2 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59914.1 hypothetical protein Csa_001231 [Cucumis sativus])

HSP 1 Score: 662 bits (1709), Expect = 9.46e-235
Identity = 338/522 (64.75%), Postives = 395/522 (75.67%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIY-GRFDAN-RKRLPPEAGGGLPVIGHLHLLA 60
           MEL  H +S    A I A I FLYA+FKIY GR  A+ RKRLPPE GG LP+IGHLHLL 
Sbjct: 7   MELL-HFSSPDVVARIFALIIFLYALFKIYRGRARAHHRKRLPPEVGGALPLIGHLHLLD 66

Query: 61  GKEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNL 120
             EPAH+TFAKMADA+GPIFT+R G+ TNLIVSNWEIARECFTTND+IFASRPKLVAS L
Sbjct: 67  KNEPAHITFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKL 126

Query: 121 LGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGRE 180
           LGYD+AMLGL PYGPHWRHVRK+  L+LLT+HRL++LQHIR+SEVQ+SIK LYELC+  +
Sbjct: 127 LGYDYAMLGLSPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNK 186

Query: 181 G---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGG---DEEYRKALRDFFEWFGM 240
               + A VEMKTWFGD+TLNTI RIVVGK+FSTAVD      +EEYRKALRDFFEWFG+
Sbjct: 187 KNNEKNALVEMKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGV 246

Query: 241 FVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNS-GQVLMEDHDFMDV 300
           FVPSD+FPFL WLDLGGH+KAMKKTA+VLDEVFDKW++EH+QK+N+ G V ME+HDFMDV
Sbjct: 247 FVPSDSFPFLKWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDV 306

Query: 301 MVSDVRDGNNGEFSRYDADTVTKATCLV-------------------------------- 360
           M+S+VRD  +G+ S+YDA  VTKATCL                                 
Sbjct: 307 MLSNVRD--DGQLSKYDAHIVTKATCLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQL 366

Query: 361 ------------------------------------SPLAVPHEAVEDCTVAGYHIPAGT 420
                                               +P+ +PHE++EDC VAGYHIP GT
Sbjct: 367 ELDEHVGRQRQVKESDVKNLLYLQAVVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGT 426

Query: 421 RLIVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFAL 445
           RLIVN+QKLQRDPQ+WEDPCEF PERFLTS+KDFDVRGQSPQLIPFG+GRR+CPGISFAL
Sbjct: 427 RLIVNVQKLQRDPQIWEDPCEFHPERFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFAL 486

BLAST of MC11g_new0040 vs. NCBI nr
Match: XP_008464765.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98756.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 659 bits (1701), Expect = 9.09e-234
Identity = 334/510 (65.49%), Postives = 387/510 (75.88%), Query Frame = 0

Query: 9   SSP-AAAGIIAGIFFLYAMFKIY--GRFDANRKRLPPEAGGGLPVIGHLHLLAGKEPAHL 68
           SSP    GI A I FLYA+FKIY  G    +RKRLPPE GG LP+IGHLHLLA  EPAH 
Sbjct: 7   SSPDTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHK 66

Query: 69  TFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAM 128
           TFAKMADA+GPIFT+R G+ TNLIVSNWEIARECFTTND+IFASRPKLVAS LLGYD+AM
Sbjct: 67  TFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAM 126

Query: 129 LGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQKAPVE 188
            GL PYGPHWRHVRK+A L+LL+N+RLE+LQHIR+SEVQ+S+K LYELC+ +  + A VE
Sbjct: 127 FGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALVE 186

Query: 189 MKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDE--EYRKALRDFFEWFGMFVPSDAFPFLS 248
           MKTWFGD+TLNTI RIVVGK+FSTAVD   +E  EYRKALRDFFEWFG+FVPSD+FPFL 
Sbjct: 187 MKTWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLK 246

Query: 249 WLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGE 308
           WLDLGGH+KAMKKTAKVLDEVFDKW+++H  K N G+V ME+HDFMDVM+S+VRD  +G+
Sbjct: 247 WLDLGGHEKAMKKTAKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRD--DGQ 306

Query: 309 FSRYDADTVTKATCL--------------------------------------------- 368
            S+YDA TVTKATCL                                             
Sbjct: 307 LSKYDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQV 366

Query: 369 -----------------------VSPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRD 428
                                   +P+ VPHE+V+DC VAGYHIP GTRLIVN+QKLQRD
Sbjct: 367 KESDVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRD 426

Query: 429 PQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLH 445
           PQ+WEDPCEFRPERFLTS KDFDVRGQ+P+LIPFG+GRR+CPGISFALQVMHL LANLLH
Sbjct: 427 PQIWEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLH 486

BLAST of MC11g_new0040 vs. ExPASy TrEMBL
Match: A0A6J1F697 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3 SV=1)

HSP 1 Score: 673 bits (1736), Expect = 2.60e-239
Identity = 345/518 (66.60%), Postives = 396/518 (76.45%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIYG-RFDANRKRLPPEAGGGLPVIGHLHLLAG 60
           MEL+   +SS   AGI+A I FLY++ KIYG  F A+R RLPPEAGG LP+IGHLH LAG
Sbjct: 1   MELSCF-SSSDVFAGILALIIFLYSLSKIYGGTFGAHRMRLPPEAGGALPLIGHLHRLAG 60

Query: 61  KEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLL 120
            EPAH++FAKMADA+GPIFTVR GV TNLIVSNWEIARECF+T DRIFASRPKLVAS LL
Sbjct: 61  NEPAHISFAKMADAYGPIFTVRLGVYTNLIVSNWEIARECFSTKDRIFASRPKLVASKLL 120

Query: 121 GYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCV-GRE 180
           GYD+AM+GL PYGPHWRHVRK+ATL+LLTN RLEQLQHIR+ EV+SSIKKLYE+CV GR 
Sbjct: 121 GYDYAMMGLSPYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRN 180

Query: 181 G---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRKALRDFFEWFGMFVP 240
               +   VEMKTWFGD+TLNTIFRIVVGKRFSTAV+G G+EEYR ALRDFFEWFG+FVP
Sbjct: 181 NGSNEGVLVEMKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVP 240

Query: 241 SDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKR-NSGQVLMEDH-DFMDVMV 300
           SD+FPFL WLDLGGH+KAMK TAKVLDEVFDKWL+EHRQK+ N G+  MED  DFMDVM+
Sbjct: 241 SDSFPFLKWLDLGGHEKAMKNTAKVLDEVFDKWLQEHRQKKKNLGEGKMEDPPDFMDVML 300

Query: 301 SDVRDGNNGEFSRYDADTVTKATCLV---------------------------------- 360
           S+V+D  +G+ S YDA TVTKATCL                                   
Sbjct: 301 SNVKD--DGQLSNYDAHTVTKATCLALILAGSDTTTITMVWALSLLLNHPEVIERAQLEL 360

Query: 361 ----------------------------------SPLAVPHEAVEDCTVAGYHIPAGTRL 420
                                             +P+ +PHE++EDCTVAGYHIPAGTRL
Sbjct: 361 DEQVGRQRQVREFDVKNLLYLQAIVKETLRLYPAAPILIPHESIEDCTVAGYHIPAGTRL 420

Query: 421 IVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQV 443
           IVN+QKLQRDPQ+W+DPCEFRPERFLT  KDFDVRGQSPQLIPFG+GRR+CPGISFALQV
Sbjct: 421 IVNVQKLQRDPQIWKDPCEFRPERFLTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQV 480

BLAST of MC11g_new0040 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 662 bits (1709), Expect = 4.58e-235
Identity = 338/522 (64.75%), Postives = 395/522 (75.67%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIY-GRFDAN-RKRLPPEAGGGLPVIGHLHLLA 60
           MEL  H +S    A I A I FLYA+FKIY GR  A+ RKRLPPE GG LP+IGHLHLL 
Sbjct: 7   MELL-HFSSPDVVARIFALIIFLYALFKIYRGRARAHHRKRLPPEVGGALPLIGHLHLLD 66

Query: 61  GKEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNL 120
             EPAH+TFAKMADA+GPIFT+R G+ TNLIVSNWEIARECFTTND+IFASRPKLVAS L
Sbjct: 67  KNEPAHITFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKL 126

Query: 121 LGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGRE 180
           LGYD+AMLGL PYGPHWRHVRK+  L+LLT+HRL++LQHIR+SEVQ+SIK LYELC+  +
Sbjct: 127 LGYDYAMLGLSPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNK 186

Query: 181 G---QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGG---DEEYRKALRDFFEWFGM 240
               + A VEMKTWFGD+TLNTI RIVVGK+FSTAVD      +EEYRKALRDFFEWFG+
Sbjct: 187 KNNEKNALVEMKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGV 246

Query: 241 FVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNS-GQVLMEDHDFMDV 300
           FVPSD+FPFL WLDLGGH+KAMKKTA+VLDEVFDKW++EH+QK+N+ G V ME+HDFMDV
Sbjct: 247 FVPSDSFPFLKWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDV 306

Query: 301 MVSDVRDGNNGEFSRYDADTVTKATCLV-------------------------------- 360
           M+S+VRD  +G+ S+YDA  VTKATCL                                 
Sbjct: 307 MLSNVRD--DGQLSKYDAHIVTKATCLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQL 366

Query: 361 ------------------------------------SPLAVPHEAVEDCTVAGYHIPAGT 420
                                               +P+ +PHE++EDC VAGYHIP GT
Sbjct: 367 ELDEHVGRQRQVKESDVKNLLYLQAVVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGT 426

Query: 421 RLIVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFAL 445
           RLIVN+QKLQRDPQ+WEDPCEF PERFLTS+KDFDVRGQSPQLIPFG+GRR+CPGISFAL
Sbjct: 427 RLIVNVQKLQRDPQIWEDPCEFHPERFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFAL 486

BLAST of MC11g_new0040 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 4.40e-234
Identity = 334/510 (65.49%), Postives = 387/510 (75.88%), Query Frame = 0

Query: 9   SSP-AAAGIIAGIFFLYAMFKIY--GRFDANRKRLPPEAGGGLPVIGHLHLLAGKEPAHL 68
           SSP    GI A I FLYA+FKIY  G    +RKRLPPE GG LP+IGHLHLLA  EPAH 
Sbjct: 7   SSPDTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHK 66

Query: 69  TFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAM 128
           TFAKMADA+GPIFT+R G+ TNLIVSNWEIARECFTTND+IFASRPKLVAS LLGYD+AM
Sbjct: 67  TFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAM 126

Query: 129 LGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQKAPVE 188
            GL PYGPHWRHVRK+A L+LL+N+RLE+LQHIR+SEVQ+S+K LYELC+ +  + A VE
Sbjct: 127 FGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALVE 186

Query: 189 MKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDE--EYRKALRDFFEWFGMFVPSDAFPFLS 248
           MKTWFGD+TLNTI RIVVGK+FSTAVD   +E  EYRKALRDFFEWFG+FVPSD+FPFL 
Sbjct: 187 MKTWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLK 246

Query: 249 WLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGE 308
           WLDLGGH+KAMKKTAKVLDEVFDKW+++H  K N G+V ME+HDFMDVM+S+VRD  +G+
Sbjct: 247 WLDLGGHEKAMKKTAKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRD--DGQ 306

Query: 309 FSRYDADTVTKATCL--------------------------------------------- 368
            S+YDA TVTKATCL                                             
Sbjct: 307 LSKYDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQV 366

Query: 369 -----------------------VSPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRD 428
                                   +P+ VPHE+V+DC VAGYHIP GTRLIVN+QKLQRD
Sbjct: 367 KESDVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRD 426

Query: 429 PQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLH 445
           PQ+WEDPCEFRPERFLTS KDFDVRGQ+P+LIPFG+GRR+CPGISFALQVMHL LANLLH
Sbjct: 427 PQIWEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLH 486

BLAST of MC11g_new0040 vs. ExPASy TrEMBL
Match: A0A1S3CM78 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 4.40e-234
Identity = 334/510 (65.49%), Postives = 387/510 (75.88%), Query Frame = 0

Query: 9   SSP-AAAGIIAGIFFLYAMFKIY--GRFDANRKRLPPEAGGGLPVIGHLHLLAGKEPAHL 68
           SSP    GI A I FLYA+FKIY  G    +RKRLPPE GG LP+IGHLHLLA  EPAH 
Sbjct: 7   SSPDTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHK 66

Query: 69  TFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAM 128
           TFAKMADA+GPIFT+R G+ TNLIVSNWEIARECFTTND+IFASRPKLVAS LLGYD+AM
Sbjct: 67  TFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAM 126

Query: 129 LGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQKAPVE 188
            GL PYGPHWRHVRK+A L+LL+N+RLE+LQHIR+SEVQ+S+K LYELC+ +  + A VE
Sbjct: 127 FGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL-KNKKNALVE 186

Query: 189 MKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDE--EYRKALRDFFEWFGMFVPSDAFPFLS 248
           MKTWFGD+TLNTI RIVVGK+FSTAVD   +E  EYRKALRDFFEWFG+FVPSD+FPFL 
Sbjct: 187 MKTWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLK 246

Query: 249 WLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGE 308
           WLDLGGH+KAMKKTAKVLDEVFDKW+++H  K N G+V ME+HDFMDVM+S+VRD  +G+
Sbjct: 247 WLDLGGHEKAMKKTAKVLDEVFDKWIQDH--KNNLGEVKMEEHDFMDVMLSNVRD--DGQ 306

Query: 309 FSRYDADTVTKATCL--------------------------------------------- 368
            S+YDA TVTKATCL                                             
Sbjct: 307 LSKYDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQV 366

Query: 369 -----------------------VSPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRD 428
                                   +P+ VPHE+V+DC VAGYHIP GTRLIVN+QKLQRD
Sbjct: 367 KESDVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRD 426

Query: 429 PQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLH 445
           PQ+WEDPCEFRPERFLTS KDFDVRGQ+P+LIPFG+GRR+CPGISFALQVMHL LANLLH
Sbjct: 427 PQIWEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLH 486

BLAST of MC11g_new0040 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 2.60e-229
Identity = 320/513 (62.38%), Postives = 380/513 (74.07%), Query Frame = 0

Query: 1   MELNSHVTSSPAAAGIIAGIFFLYAMFKIYGRFDANRKRLPPEAGGGLPVIGHLHLLAGK 60
           MEL  H +SS A AGI A + FLY +F I  R  +N KRLPPE  G  PVIGHLHLL+  
Sbjct: 1   MELP-HTSSSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTT 60

Query: 61  EPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLG 120
           EPAH+T AKMAD +GPIFT+R G+   LIVS+WEIARECFTT DRIFASRPKLVAS LLG
Sbjct: 61  EPAHITLAKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLG 120

Query: 121 YDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREGQ 180
           Y++AM+GL PYG HWRHVRK+ATL+L TNHRL+QLQH+R+SEV SSIKKLYELC G   +
Sbjct: 121 YNYAMMGLSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELC-GNGKE 180

Query: 181 KAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRKALRDFFEWFGMFVPSDAFP 240
           KA V+M TWFGD+TLNTIFR+VVGKRFSTAV+GGG++EYRKALR+FFEWFG+FVPSD+FP
Sbjct: 181 KALVDMTTWFGDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFP 240

Query: 241 FLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGN 300
           FL WLDLGGH+KAMKKTA+VLDEVFDKW++E R+ R S +    +HDFMDVM+S V+D +
Sbjct: 241 FLKWLDLGGHEKAMKKTAEVLDEVFDKWVEERRKMRASDEAKTAEHDFMDVMLSTVKDCD 300

Query: 301 NGEFSRYDADTVTKATCLV----------------------------------------- 360
             E S YDA+ V K+TCL                                          
Sbjct: 301 --ELSSYDANIVIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALEKARLELDEQVGRE 360

Query: 361 ---------------------------SPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKL 420
                                       PL +PHE++EDCT+  YHIPAGTRL+VN+QKL
Sbjct: 361 THVKESDVKNLIYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKL 420

Query: 421 QRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLAN 445
           QRDP+VWEDPCEFRPERFLT+ +DF+VRGQSP+ IPFGNGRR+CPGISFALQ+MHLTLAN
Sbjct: 421 QRDPRVWEDPCEFRPERFLTNHRDFNVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLAN 480

BLAST of MC11g_new0040 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-101
Identity = 196/484 (40.50%), Postives = 272/484 (56.20%), Query Frame = 0

Query: 42  PEAGGGLPVIGHLHLLAGKEP-AHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECF 101
           P   G  P+IGHLHLL GKE   + T  KMAD +GP  +++ G +   +VS++E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 102 TTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRI 161
           T ND+  ASRP   A+  +GY+FA+ G  PY   WR +RKIAT++LL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 162 SEVQSSIKKLYELCVGREGQK-APVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGD--- 221
           SE+   +K LY L     G K   V++K+W  D+TLN I R+V GKR+     GGG    
Sbjct: 153 SEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSS 212

Query: 222 ------EEYRKALRDFFEWFGMFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLK 281
                  + +KA+  FF   G+F  SDAFP LS+ DL GH+K MK+T   LD + ++W++
Sbjct: 213 EDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIE 272

Query: 282 EHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYDADTVTKATCL------------ 341
            HRQ+R        D DF+DVM+S    G      +YDA+T  K+TCL            
Sbjct: 273 NHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHL-QYDANTSIKSTCLALILGGSDTSAS 332

Query: 342 --------------------------------------------------------VSPL 401
                                                                     PL
Sbjct: 333 TLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPL 392

Query: 402 AVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWEDPCEFRPERFLTSQ-KDFDVRG 446
             P EA+EDCTVAGY++P GTRLIVN+ K+QRDP+V+ +P EFRPERF+T + K+FDVRG
Sbjct: 393 LGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRG 452

BLAST of MC11g_new0040 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 354.8 bits (909), Expect = 1.0e-97
Identity = 193/506 (38.14%), Postives = 278/506 (54.94%), Query Frame = 0

Query: 20  IFFLYAMFKIYGRFDANRKRLPPEAGGGLPVIGHLHLLAGKEP-AHLTFAKMADAFGPIF 79
           +F   A+FK   +    +    P   G  P+IGHLHLL+GKE   + T  KMAD +GP  
Sbjct: 14  VFVFIALFK---KSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAM 73

Query: 80  TVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPHWRHV 139
           ++R G S   +VS++E+A++CFT ND+  ASRP   A+  +GYD A+ G  PY   WR +
Sbjct: 74  SLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREM 133

Query: 140 RKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCVGREG-QKAPVEMKTWFGDVTLNT 199
           RKIATL+LL+N RL+ L+H+R+SE+   ++ LY L V + G +   V++K+W  D++LN 
Sbjct: 134 RKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNM 193

Query: 200 IFRIVVGKRFSTAVDGGGD---------EEYRKALRDFFEWFGMFVPSDAFPFLSWLDLG 259
           + R+V GKR+     GGG           + RK + +FF   G+F  SDAFP L W D  
Sbjct: 194 MVRMVAGKRYF----GGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQ 253

Query: 260 GHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYD 319
           GH+K MK+T + LD + ++W++ HRQ+R        D DF+DVM+S    G      ++D
Sbjct: 254 GHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHL-QHD 313

Query: 320 ADTVTKATCL-------------------------------------------------- 379
           A T  K+TCL                                                  
Sbjct: 314 AITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDI 373

Query: 380 ------------------VSPLAVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWE 439
                               PL    EA+EDCTVAGY++  GTR++VN+ K+QRDP+V+ 
Sbjct: 374 ENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYM 433

Query: 440 DPCEFRPERFLTSQ-KDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLHGYEI 446
           +P EFRPERF+T + K+FDVRGQ+ +L+PFG+GRR CPG S A+QV+HL LA  L  +++
Sbjct: 434 EPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDV 493

BLAST of MC11g_new0040 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 335.5 bits (859), Expect = 6.5e-92
Identity = 188/484 (38.84%), Postives = 262/484 (54.13%), Query Frame = 0

Query: 42  PEAGGGLPVIGHLHLLAGKEP-AHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECF 101
           P   G  P+IGHLHLL GKE   + T  KMAD +GP  ++R G S   + S++E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCF 92

Query: 102 TTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRI 161
           T ND+  AS     A + +GY F           W  +RKIA ++LL+N RL+ L ++R+
Sbjct: 93  TVNDKALASLMTAAAKH-MGYVF-----------WLEMRKIAMIELLSNRRLQMLNNVRV 152

Query: 162 SEVQSSIKKLYELCVGREG-QKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDE-- 221
           SE+   +K LY L V + G +   V++K+W  D+  N I R+V GKR+     GGG E  
Sbjct: 153 SEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIANMIMRMVAGKRY---FGGGGAESS 212

Query: 222 -------EYRKALRDFFEWFGMFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLK 281
                  ++RK +  FF   G+F  SDAFP L WLDL GH+K MK+T + LD + ++W++
Sbjct: 213 EHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIE 272

Query: 282 EHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYDADTVTKATCL------------ 341
            HRQ+R        D DF+DVM+S    G      +YDA+T  K TCL            
Sbjct: 273 NHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHL-QYDANTCIKTTCLALILGGSETSPS 332

Query: 342 --------------------------------------------------------VSPL 401
                                                                    +PL
Sbjct: 333 TLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPL 392

Query: 402 AVPHEAVEDCTVAGYHIPAGTRLIVNLQKLQRDPQVWEDPCEFRPERFLTSQ-KDFDVRG 446
               EA+EDCTVAGY++P GTRLIVN+ K+QRDP+V+ +P EFRPERF+T + KDFDVRG
Sbjct: 393 LGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRG 452

BLAST of MC11g_new0040 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 259.2 bits (661), Expect = 6.0e-69
Identity = 158/472 (33.47%), Postives = 234/472 (49.58%), Query Frame = 0

Query: 42  PEAGGGLPVIGHLHLLAGKEPAHLTFAKMADAFGPIFTVRFGVSTNLIVSNWEIARECFT 101
           PE  G LP+ GHLHLL GK+      A M+   GPIF+++ G    ++ S+ +  ++CFT
Sbjct: 40  PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99

Query: 102 TNDRIFASRPKLVASNLLGYDFAMLGLGPYGPHWRHVRKIATLQLLTNHRLEQLQHIRIS 161
           TND   A+RP +     +GY+ A L L PYG +WR +RKI T+ L +NH +E L HIR S
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSS 159

Query: 162 EVQSSIKKLYELCVGREGQKAPVEMKTWFGDVTLNTIFRIVVGKRFSTAVDGGGDEEYRK 221
           EV + IK LY+      G  + V++   F  +T N I R +VGKR         +  Y++
Sbjct: 160 EVNTLIKHLYK----GNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE 219

Query: 222 ALRDFFEWFGMFVPSDAFPFLSWLDLGGHQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQV 281
           AL+       + +  D  P+L WLD   + + MK+  K LD V  KWL EH +KR+  + 
Sbjct: 220 ALKHCEYLAVIPMIGDVIPWLGWLDFAKNSQ-MKRLFKELDSVNTKWLHEHLKKRSRNE- 279

Query: 282 LMEDHDFMDVMVSDVRDGNNGEFSRYDADTVTKATCLV---------------------- 341
             ++   MD+++  + +  +   S +  D + KAT L                       
Sbjct: 280 KDQERTIMDLLLDILPE--DIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLN 339

Query: 342 ----------------------------------------------SPLAVPHEAVEDCT 401
                                                         +PL    EA EDC 
Sbjct: 340 NPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCF 399

Query: 402 VAGYHIPAGTRLIVNLQKLQRDPQVWEDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGR 446
           V GY +  GTRL+VN+ KL RDP++W DP  F+PERF+  +   +    + + IPFG+GR
Sbjct: 400 VGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGR 459

BLAST of MC11g_new0040 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 257.3 bits (656), Expect = 2.3e-68
Identity = 159/506 (31.42%), Postives = 248/506 (49.01%), Query Frame = 0

Query: 16  IIAGIFFLYAMFKIYGRF-DANRKRLPPEAGGGLPVIGHLHLLAGKEPAHLTFAKMADAF 75
           +++ +F    +  I  R    N+K   P   G  P++GHLHL     P H+TF  MAD +
Sbjct: 8   LLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVY 67

Query: 76  GPIFTVRFGVSTNLIVSNWEIARECFTTNDRIFASRPKLVASNLLGYDFAMLGLGPYGPH 135
           GP+F  + G    +I+++ E+A+E +T +D++   RP+L AS LLGY+ + L   PYG +
Sbjct: 68  GPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLY 127

Query: 136 WRHVRKIATLQLLTNHRLEQLQHIRISEVQSSIKKLYELCV--GREGQKAPVEMKTWFGD 195
           WR +RKIA  +L +   ++     R  E   + + LY      G+  +   V+MK  F D
Sbjct: 128 WREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFID 187

Query: 196 VTLNTIFRIVVGKRFSTAVDGGGDEEYR---KALRDFFEWFGMFVPSDAFPFLSWLDLGG 255
           +T N    +V GKR+         +E R   K +R+F ++F +F+ SD  P L +LD   
Sbjct: 188 LTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-WK 247

Query: 256 HQKAMKKTAKVLDEVFDKWLKEHRQKRNSGQVLMEDHDFMDVMVSDVRDGNNGEFSRYDA 315
            ++ MK+TAK LD+V + W++EH+ KR+       ++D++D+++  +  G +      D 
Sbjct: 248 TKRGMKRTAKGLDKVAEGWIEEHKNKRSDHG--RSENDYLDILIKIL--GQDKIPGLSDT 307

Query: 316 DTVTKATCL-----------------VS-------------------------------- 375
            T  KA CL                 VS                                
Sbjct: 308 HTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIK 367

Query: 376 -------------------PLAVPHEAVEDCTVA--GYHIPAGTRLIVNLQKLQRDPQVW 435
                              PL      VED  +A    H+PAGT+L+V+  K+ RDP VW
Sbjct: 368 DLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVW 427

Query: 436 EDPCEFRPERFLTSQKDFDVRGQSPQLIPFGNGRRICPGISFALQVMHLTLANLLHGYEI 446
            +P +F PERFLTS ++ DV GQS +  PFG GRR CP I   ++++H  L   LH +++
Sbjct: 428 SNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDL 487