Homology
BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match:
Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)
HSP 1 Score: 506.1 bits (1302), Expect = 5.0e-142
Identity = 264/533 (49.53%), Postives = 363/533 (68.11%), Query Frame = 0
Query: 39 MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
M+ S+F I V +F ALF S+K + P GA P+IGHLHLL KE
Sbjct: 1 MDTSLFSLFVPILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60
Query: 99 -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
+ TL KMAD YGP +L+ G +A VVSS+E+A++ FT ND+ ASRP A+K +GY
Sbjct: 61 LLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGY 120
Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKS--EE 218
++A+FG +PY WR+ R++AT+ELL+N RL+ L+HVR+ E+ +K+LY L K+ +
Sbjct: 121 NFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK 180
Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGS---------EECKKALRDFFELFG 278
+V++K W D+TL+ I R+V GKR+ F G GS +CKKA+ FF L G
Sbjct: 181 PVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIG 240
Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
IF SD+FP LS+ DL GHEK MK T LD ++++++H Q F+ ++E D DF++V
Sbjct: 241 IFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDV 300
Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
M+S A + + YDANT IK+TCL +ILGG DT+ T+TW ISLLLNN+E L+KAQ E
Sbjct: 301 MMSLAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDE 360
Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
+D VGR R V++SD++NL+YLQAI+KETLRLYPA PLL P E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTR 420
Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
LIVNV K+QRDP V+ + EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAM 480
Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
QV+HL LA LH F + + P+DM E+ GLT + PLEV+++P + +++
Sbjct: 481 QVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523
BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match:
O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)
HSP 1 Score: 479.6 bits (1233), Expect = 5.0e-134
Identity = 253/529 (47.83%), Postives = 355/529 (67.11%), Query Frame = 0
Query: 39 MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
M+ S+F I V +F ALF S+K + P P GA P+IGHLHLLS KE
Sbjct: 1 MDTSLFSLFVPILVFVFI--ALFKKSKK--PKHVKAPAP---SGAWPIIGHLHLLSGKEQ 60
Query: 99 -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
+ TL KMAD YGP +LR G + VVSS+E+A++ FT ND+ ASRP A+K +GY
Sbjct: 61 LLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGY 120
Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
D A+FG +PY WR+ R++ATLELL+N RL+ L+HVR+ E+ +++LY L VK E
Sbjct: 121 DCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE 180
Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRF--SAAFNGSGSEE---CKKALRDFFELFGIFIP 278
+V++K W D++L+ + R+V GKR+ + + +EE C+K + +FF L GIF
Sbjct: 181 PVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 240
Query: 279 SDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMIS- 338
SD+FP L W D GHEK MK T + LD ++++++H Q + ++ D DF++VM+S
Sbjct: 241 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 300
Query: 339 AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQ 398
A + + +DA T IK+TCL +ILGG +T+ T+TW ISLLLNN++ L+KAQ E+D
Sbjct: 301 AEQGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 360
Query: 399 VGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVN 458
VGR R V++SD++NL+Y+QAI+KETLRLYPA PLL E+IEDCT AGY++ GTR++VN
Sbjct: 361 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 420
Query: 459 VQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFALQVIH 518
V K+QRDP V+ + EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+QV+H
Sbjct: 421 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 480
Query: 519 LTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
L LA L F + + P+DM ES GLT + PLE++++P L +Y
Sbjct: 481 LGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match:
A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)
HSP 1 Score: 476.1 bits (1224), Expect = 5.6e-133
Identity = 249/513 (48.54%), Postives = 336/513 (65.50%), Query Frame = 0
Query: 45 SIFTGIFVLL-FFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 104
++ G FVLL F Y ++ ++ PP+ GA P+IGHLHLL+ + H L
Sbjct: 9 TLLLGFFVLLPCFFYFVWK------KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKIL 68
Query: 105 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 164
+ AD GP+F ++ G+ +ALVV++ EIA+E FTTNDR F +RP VA+K++GY+Y M G
Sbjct: 69 SSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLG 128
Query: 165 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLC-VKSEEKAVVEMK 224
++PYGP+WR R++ LE L+N RL+ L+HV E+ KELYKL ++ + +V+MK
Sbjct: 129 VAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMK 188
Query: 225 KWFGDLTLDTIFRIVVGKRFSAAFNGSGSEE-----CKKALRDFFELFGIFIPSDSFPFL 284
+W DLTL+ ++VVGKRF + + S EE C K LR+ F L G F+ SD P+L
Sbjct: 189 QWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYL 248
Query: 285 SWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGES 344
WLDLGGHEK MK T K LD F +L +H + E+ DQDFM+VM+S + E
Sbjct: 249 RWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSIL--EESK 308
Query: 345 FSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVK 404
D +T+ K CL +ILGG DTT T+TW +SLLLNN L+KAQ ELD VGR R V
Sbjct: 309 LGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVD 368
Query: 405 ESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDP 464
ESD+ L Y+ AI+KETLRLYP PLL P EDCT AGYH+ GTRLIVN K+QRDP
Sbjct: 369 ESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDP 428
Query: 465 LVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHG 524
LVW E++PERFL +++ D++GQ +LIPF +GRR CP IS ALQV+ LTLA++LHG
Sbjct: 429 LVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHG 488
Query: 525 FKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTP 551
F++ P++ +DM E+ G+ + PLEV++ P
Sbjct: 489 FELRTPNQNKVDMTETPGIVHAKATPLEVLVAP 511
BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match:
O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)
HSP 1 Score: 475.3 bits (1222), Expect = 9.5e-133
Identity = 232/480 (48.33%), Postives = 328/480 (68.33%), Query Frame = 0
Query: 78 PEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFT 137
P GA P++GHL LL+ + H L +AD YGP+FT++ GMK ALV+S+WE+++E FT
Sbjct: 39 PVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFT 98
Query: 138 TNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRIL 197
TND +SRPKLVA +++ Y+ A GL+PYGP+WR+ R++ T E L+N R+E+ H+R+
Sbjct: 99 TNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVS 158
Query: 198 EVKNWIKELYKLCV---KSEEK-AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEE 257
EV+ IKEL+ + K+E + +V++K+W LT + + R+VVGKR+ + G ++
Sbjct: 159 EVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDK 218
Query: 258 CK---KALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQT 317
+ K +R+F L G F +D P L WLDLGGHEKAMK AK +D ++L++H Q
Sbjct: 219 AQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQK 278
Query: 318 TNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVIS 377
+ E+D+DFM+VMISA+ N + ++DA+T+ KAT L +ILGG D+T VT+TW +S
Sbjct: 279 KLLGENVESDRDFMDVMISAL-NGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALS 338
Query: 378 LLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESI 437
LLL N L KA+ E+D Q+G+ ++ESD+ L+YLQAIVKETLRLYP AP P E
Sbjct: 339 LLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFT 398
Query: 438 EDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPF 497
E+C GYHI GTRLI N+ K+ RDP VW D EF+PERFLT+ K+ D+RG + +L+PF
Sbjct: 399 ENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPF 458
Query: 498 RNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTP 551
+GRR+C G+S L ++H TLANLLH F I PS EP+DM E G T+ + PLE+++ P
Sbjct: 459 GSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKP 517
BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match:
O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)
HSP 1 Score: 461.5 bits (1186), Expect = 1.4e-128
Identity = 240/519 (46.24%), Postives = 340/519 (65.51%), Query Frame = 0
Query: 44 VSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 103
+ + + IF+L FLY + + G+ + P GGA P+ GHL LL + H L
Sbjct: 13 IGVVSLIFLLCLFLYG--PLKKVHGSSKE----APTVGGAWPIFGHLPLLIGSKSPHKAL 72
Query: 104 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 163
+A+ +GP+FT++ G KKALVVS WE+ARE FTTND ++RPKL+ ++L+ Y+ AM
Sbjct: 73 GALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLL 132
Query: 164 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKL--CVKSE-EKAVVE 223
++PYGP+WR+ R++ E+L++ R+E+LQ VR+ EV+N I ELY + K+E + A VE
Sbjct: 133 VAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVE 192
Query: 224 MKKWFGDLTLDTIFRIVVGKRF-SAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSW 283
+K+WF + + R+VVGKRF SA +E+C KA+ +F L G+F D+ P+L W
Sbjct: 193 LKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRW 252
Query: 284 LDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESF- 343
LD GG+EKAMK TAK LD ++L++H Q + QDFM VM+S++ +G++
Sbjct: 253 LDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSL--DGKTID 312
Query: 344 SYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKE 403
DA+T+IK+T L +I G + + T+ W + L+L N L+ + ELD QVG+ R + E
Sbjct: 313 GIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICE 372
Query: 404 SDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPL 463
SD+ NL+YLQA+VKETLRLY PL P E EDCT GYH+ GTRLI N+ K+ DP
Sbjct: 373 SDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPN 432
Query: 464 VWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGF 523
VW D EF+P+RFLT+ K+ DV+G QL+PF +GRR+CPGISF LQ +HL LA+ LH F
Sbjct: 433 VWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSF 492
Query: 524 KIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
+I PS EPLDM E+ G+T+ + PLEV++ PCL Y
Sbjct: 493 EILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522
BLAST of MC11g_new0035 vs. NCBI nr
Match:
XP_022138580.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 1147 bits (2967), Expect = 0.0
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0
Query: 1 MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL
Sbjct: 1 MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
Query: 61 FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM
Sbjct: 61 FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
Query: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL
Sbjct: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
Query: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK
Sbjct: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
Query: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF
Sbjct: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
Query: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT
Sbjct: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
Query: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA
Sbjct: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
Query: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV
Sbjct: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
Query: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR
Sbjct: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
Query: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
KAPLEVVLTPCLPAQVYGKVCNKIMNKK
Sbjct: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
BLAST of MC11g_new0035 vs. NCBI nr
Match:
XP_022138438.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0
Query: 39 MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98
MEV HV IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 1 MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
Query: 99 EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158
EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 61 EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
Query: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218
YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
Query: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278
AVVEMK WFGD+TL+TIFR+VVGKRFS AF+ +GSEECKKALRDFF LFGIFIPSD+F
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
Query: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338
FLSW DLGGHEKAMK TA+LLD FDKFLK+H Q +NF SE +QDFM+VMIS VKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
Query: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398
E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
Query: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458
R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
Query: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518
QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
Query: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
BLAST of MC11g_new0035 vs. NCBI nr
Match:
XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 770 bits (1988), Expect = 1.91e-275
Identity = 387/521 (74.28%), Postives = 438/521 (84.07%), Query Frame = 0
Query: 41 VPHVSIFTGI-FVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPT 100
+ H+ GI F L FLY F SR+S A R+R +PPEAGGA PVIGHLHLL A EPT
Sbjct: 1 MEHLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKR--IPPEAGGAWPVIGHLHLLHAGEPT 60
Query: 101 HITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDY 160
HITLAKMADAYGP+FT RFGMKKAL+VSSWEIA+EFFTTNDR+FASRPKLVASKLL YDY
Sbjct: 61 HITLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDY 120
Query: 161 AMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEE---K 220
AM G SPY PHWRQ R++ATLELLTNHRLE+LQH+R EV+ W+ EL+K+ +KS E K
Sbjct: 121 AMIGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSIKSNERGEK 180
Query: 221 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPF 280
VEMKKW D+TL+TIF++V+GKRFSA +G+ E KAL DFF LF IF+PSDSFPF
Sbjct: 181 VAVEMKKWLADITLNTIFKMVIGKRFSAIQHGN--ENFPKALIDFFGLFHIFVPSDSFPF 240
Query: 281 LSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGE 340
LSWLDLGGHEK MK TAK++D AF KFL+ H + N A +E A+QDFM+VMIS V+ +G+
Sbjct: 241 LSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNDAEAETAEQDFMDVMISRVEEDGQ 300
Query: 341 SFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREV 400
FSYDA+TVIKAT LNM+L GFDTTT+TM W +SLLLNN+ L+ AQLELDE+VGR R+V
Sbjct: 301 HFSYDADTVIKATSLNMVLAGFDTTTITMVWALSLLLNNEGVLENAQLELDEKVGRSRQV 360
Query: 401 KESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRD 460
KESDVKNL YL+AIVKETLRLYPAAPLLVPHESIEDCTAAGYHI GTRLIVNVQKLQ+D
Sbjct: 361 KESDVKNLPYLRAIVKETLRLYPAAPLLVPHESIEDCTAAGYHISKGTRLIVNVQKLQKD 420
Query: 461 PLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLH 520
PLVWE CEFRPERFLTSQK+FDVRGQSPQ IPF NGRRMCP ISFALQ+IHLTLAN LH
Sbjct: 421 PLVWEGPCEFRPERFLTSQKDFDVRGQSPQFIPFGNGRRMCPAISFALQMIHLTLANFLH 480
Query: 521 GFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
GFKI+R S+EPLDMEESVGLTS+RKAPLEVVLTP LPA VY
Sbjct: 481 GFKIERSSDEPLDMEESVGLTSLRKAPLEVVLTPRLPAHVY 517
BLAST of MC11g_new0035 vs. NCBI nr
Match:
XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 765 bits (1975), Expect = 1.75e-273
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0
Query: 50 IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
IF LLFFLYALF +SR+S A R++LP PE GGA PVIGHLHLLS KEPTHITL+K+AD
Sbjct: 10 IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69
Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70 YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129
Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
HWR AR++ATLELLTNHRLE+LQH+R EV+ W +LY+L VK +EK VVEMK F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189
Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
TL+TIF +V+GKRFS AF+G E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249
Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
MK TAK+LD AF+KFLK+H Q + E ++DFM+VMIS V +G FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309
Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369
Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP WE+ EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429
Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
+RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489
Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516
BLAST of MC11g_new0035 vs. NCBI nr
Match:
XP_022940485.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 765 bits (1975), Expect = 1.75e-273
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0
Query: 50 IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
IF LLFFLYALF +SR+S A R++LP PE GGA PVIGHLHLLS KEPTHITL+K+AD
Sbjct: 10 IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69
Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70 YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129
Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
HWR AR++ATLELLTNHRLE+LQH+R EV+ W +LY+L VK +EK VVEMK F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189
Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
TL+TIF +V+GKRFS AF+G E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249
Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
MK TAK+LD AF+KFLK+H Q + E ++DFM+VMIS V +G FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309
Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369
Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP WE+ EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429
Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
+RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489
Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516
BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match:
A0A6J1C9U5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=3 SV=1)
HSP 1 Score: 1147 bits (2967), Expect = 0.0
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0
Query: 1 MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL
Sbjct: 1 MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
Query: 61 FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM
Sbjct: 61 FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
Query: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL
Sbjct: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
Query: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK
Sbjct: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
Query: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF
Sbjct: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
Query: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT
Sbjct: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
Query: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA
Sbjct: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
Query: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV
Sbjct: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
Query: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR
Sbjct: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
Query: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
KAPLEVVLTPCLPAQVYGKVCNKIMNKK
Sbjct: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match:
A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)
HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0
Query: 39 MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98
MEV HV IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 1 MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
Query: 99 EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158
EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 61 EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
Query: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218
YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
Query: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278
AVVEMK WFGD+TL+TIFR+VVGKRFS AF+ +GSEECKKALRDFF LFGIFIPSD+F
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
Query: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338
FLSW DLGGHEKAMK TA+LLD FDKFLK+H Q +NF SE +QDFM+VMIS VKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
Query: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398
E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
Query: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458
R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
Query: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518
QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
Query: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match:
A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)
HSP 1 Score: 765 bits (1975), Expect = 8.45e-274
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0
Query: 50 IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
IF LLFFLYALF +SR+S A R++LP PE GGA PVIGHLHLLS KEPTHITL+K+AD
Sbjct: 10 IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69
Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70 YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129
Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
HWR AR++ATLELLTNHRLE+LQH+R EV+ W +LY+L VK +EK VVEMK F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189
Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
TL+TIF +V+GKRFS AF+G E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249
Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
MK TAK+LD AF+KFLK+H Q + E ++DFM+VMIS V +G FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309
Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369
Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP WE+ EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429
Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
+RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489
Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516
BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match:
A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)
HSP 1 Score: 765 bits (1975), Expect = 8.45e-274
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0
Query: 50 IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
IF LLFFLYALF +SR+S A R++LP PE GGA PVIGHLHLLS KEPTHITL+K+AD
Sbjct: 10 IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69
Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70 YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129
Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
HWR AR++ATLELLTNHRLE+LQH+R EV+ W +LY+L VK +EK VVEMK F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189
Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
TL+TIF +V+GKRFS AF+G E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249
Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
MK TAK+LD AF+KFLK+H Q + E ++DFM+VMIS V +G FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309
Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369
Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP WE+ EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429
Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
+RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489
Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516
BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match:
A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)
HSP 1 Score: 756 bits (1951), Expect = 5.06e-270
Identity = 378/529 (71.46%), Postives = 437/529 (82.61%), Query Frame = 0
Query: 41 VPHVSIFTGI-FVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPT 100
+ H+ GI F LFF+Y LF SR+S A R+R +PPEAGGA PVIGHLHLL A +PT
Sbjct: 1 MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKR--IPPEAGGAWPVIGHLHLLHAGDPT 60
Query: 101 HITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDY 160
HI LAKMADAYGPMFTLRFGMKKALVVSSWEIA+EF TTNDR+FASRPKLVASKLL YDY
Sbjct: 61 HIALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDY 120
Query: 161 AMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEE---K 220
AM G SPY PHWRQ R++ATLELLTNHRLE+LQH+R+ EV+ W+KEL+K+ ++S E K
Sbjct: 121 AMMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDK 180
Query: 221 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPF 280
VEMKKW D+TL+TIF++V+GKRFSA +G+ E KAL DFF LF IF+PSDSFPF
Sbjct: 181 VAVEMKKWLADITLNTIFKMVIGKRFSAIQHGT--ENFPKALIDFFGLFHIFVPSDSFPF 240
Query: 281 LSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGE 340
LSWLDLGGHEK MK TAK++D AF KFL+ H + N +E A+QDFM+VMIS V+ + +
Sbjct: 241 LSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNEGQAETAEQDFMDVMISRVEEDEQ 300
Query: 341 SFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREV 400
FSYDA+TVIKATCLNMI+GGFDTT VTMTW + LLLNN+E L+K QLELDE+VGR +V
Sbjct: 301 HFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQV 360
Query: 401 KESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRD 460
KESDVKNL YLQAIVKETLRLYPA PL+ PHES+EDCT AGYHI GTRLIVNVQKLQ+D
Sbjct: 361 KESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKD 420
Query: 461 PLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLH 520
P VWE+ EF PERF+TSQKNFDVRG SPQ IPF NGRRMCP ISFALQV+H+TLAN LH
Sbjct: 421 PRVWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANFLH 480
Query: 521 GFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVYGKVCNKIM 565
GF+I+RPSEE LDMEE +GLTS+RKAPLEVVLTP LPA +Y N+I+
Sbjct: 481 GFEIERPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIYESSRNRIL 525
BLAST of MC11g_new0035 vs. TAIR 10
Match:
AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )
HSP 1 Score: 506.1 bits (1302), Expect = 3.6e-143
Identity = 264/533 (49.53%), Postives = 363/533 (68.11%), Query Frame = 0
Query: 39 MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
M+ S+F I V +F ALF S+K + P GA P+IGHLHLL KE
Sbjct: 1 MDTSLFSLFVPILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60
Query: 99 -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
+ TL KMAD YGP +L+ G +A VVSS+E+A++ FT ND+ ASRP A+K +GY
Sbjct: 61 LLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGY 120
Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKS--EE 218
++A+FG +PY WR+ R++AT+ELL+N RL+ L+HVR+ E+ +K+LY L K+ +
Sbjct: 121 NFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK 180
Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGS---------EECKKALRDFFELFG 278
+V++K W D+TL+ I R+V GKR+ F G GS +CKKA+ FF L G
Sbjct: 181 PVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIG 240
Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
IF SD+FP LS+ DL GHEK MK T LD ++++++H Q F+ ++E D DF++V
Sbjct: 241 IFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDV 300
Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
M+S A + + YDANT IK+TCL +ILGG DT+ T+TW ISLLLNN+E L+KAQ E
Sbjct: 301 MMSLAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDE 360
Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
+D VGR R V++SD++NL+YLQAI+KETLRLYPA PLL P E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTR 420
Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
LIVNV K+QRDP V+ + EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAM 480
Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
QV+HL LA LH F + + P+DM E+ GLT + PLEV+++P + +++
Sbjct: 481 QVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523
BLAST of MC11g_new0035 vs. TAIR 10
Match:
AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )
HSP 1 Score: 479.6 bits (1233), Expect = 3.6e-135
Identity = 253/529 (47.83%), Postives = 355/529 (67.11%), Query Frame = 0
Query: 39 MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
M+ S+F I V +F ALF S+K + P P GA P+IGHLHLLS KE
Sbjct: 1 MDTSLFSLFVPILVFVFI--ALFKKSKK--PKHVKAPAP---SGAWPIIGHLHLLSGKEQ 60
Query: 99 -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
+ TL KMAD YGP +LR G + VVSS+E+A++ FT ND+ ASRP A+K +GY
Sbjct: 61 LLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGY 120
Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
D A+FG +PY WR+ R++ATLELL+N RL+ L+HVR+ E+ +++LY L VK E
Sbjct: 121 DCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE 180
Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRF--SAAFNGSGSEE---CKKALRDFFELFGIFIP 278
+V++K W D++L+ + R+V GKR+ + + +EE C+K + +FF L GIF
Sbjct: 181 PVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 240
Query: 279 SDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMIS- 338
SD+FP L W D GHEK MK T + LD ++++++H Q + ++ D DF++VM+S
Sbjct: 241 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 300
Query: 339 AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQ 398
A + + +DA T IK+TCL +ILGG +T+ T+TW ISLLLNN++ L+KAQ E+D
Sbjct: 301 AEQGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 360
Query: 399 VGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVN 458
VGR R V++SD++NL+Y+QAI+KETLRLYPA PLL E+IEDCT AGY++ GTR++VN
Sbjct: 361 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 420
Query: 459 VQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFALQVIH 518
V K+QRDP V+ + EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+QV+H
Sbjct: 421 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 480
Query: 519 LTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
L LA L F + + P+DM ES GLT + PLE++++P L +Y
Sbjct: 481 LGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC11g_new0035 vs. TAIR 10
Match:
AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )
HSP 1 Score: 454.9 bits (1169), Expect = 9.4e-128
Identity = 248/533 (46.53%), Postives = 343/533 (64.35%), Query Frame = 0
Query: 39 MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
M+ S+F I V +F ALF S+K + P GA P+IGHLHLL KE
Sbjct: 1 MDTSLFSLFVSILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60
Query: 99 -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
+ TL KMAD YGP +LR G + V SS+E+A++ FT ND+ AS A+K +GY
Sbjct: 61 LLYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY 120
Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
+ W + R++A +ELL+N RL+ L +VR+ E+ +K+LY L VK E
Sbjct: 121 VF-----------WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSE 180
Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSE---------ECKKALRDFFELFG 278
+V++K W D+ + I R+V GKR+ F G G+E + +K + FF L G
Sbjct: 181 PVMVDLKSWLEDMIANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVG 240
Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
IF SD+FP L WLDL GHEK MK T + LD ++++++H Q + ++ D DF++V
Sbjct: 241 IFTVSDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDV 300
Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
M+S A + + YDANT IK TCL +ILGG +T+ T+TW ISLLLNN++ L+K Q E
Sbjct: 301 MLSLAEQGKLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDE 360
Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
+D VGR R V++SD+KNL+YLQAI+KETLRLYPAAPLL E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTR 420
Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
LIVNV K+QRDP V+ + EFRPERF+T + K+FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAM 480
Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
Q++HL LA LH F++ + P+DM ES GLT + PLEV++ P L +++
Sbjct: 481 QMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511
BLAST of MC11g_new0035 vs. TAIR 10
Match:
AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )
HSP 1 Score: 361.7 bits (927), Expect = 1.1e-99
Identity = 203/520 (39.04%), Postives = 302/520 (58.08%), Query Frame = 0
Query: 52 VLLFFLYALF-----NMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKM 111
++LF L ALF + + + + P GA P++GHLHL PTH+T M
Sbjct: 4 IMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAM 63
Query: 112 ADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSP 171
AD YGP+F + G K ++++S E+A+E +T +D+L RP+L ASKLLGY+ + SP
Sbjct: 64 ADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSP 123
Query: 172 YGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELY---KLCVKSEEKAVVEMKK 231
YG +WR+ R++A EL + ++ R E + LY + K +E +V+MK+
Sbjct: 124 YGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQ 183
Query: 232 WFGDLTLDTIFRIVVGKRFSAAFNGSGSEE---CKKALRDFFELFGIFIPSDSFPFLSWL 291
F DLT + +V GKR+ +E C K +R+F + F +F+ SD P L +L
Sbjct: 184 EFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFL 243
Query: 292 DLGGHEKAMKHTAKLLDHAFDKFLKDH-NQTTNFAASEEADQDFMEVMISAVKNEGESFS 351
D ++ MK TAK LD + ++++H N+ ++ SE D+++++I + +
Sbjct: 244 D-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSE---NDYLDILIKILGQDKIPGL 303
Query: 352 YDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKES 411
D +T IKA CLN++L G +T V + W +SLLLNN L+KAQ ELD ++G+ R V+E
Sbjct: 304 SDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEEL 363
Query: 412 DVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAA--GYHIPIGTRLIVNVQKLQRDP 471
D+K+L+YLQAIVKET RLYP PL+ +ED A H+P GT+L+V+ K+ RDP
Sbjct: 364 DIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDP 423
Query: 472 LVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHG 531
VW + +F PERFLTS + DV GQS + PF GRR CP I ++++H L LH
Sbjct: 424 NVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHS 483
Query: 532 FKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
F + RPS + +DM ES GL + + PLEV + P L +Y
Sbjct: 484 FDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLY 518
BLAST of MC11g_new0035 vs. TAIR 10
Match:
AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )
HSP 1 Score: 352.8 bits (904), Expect = 5.0e-97
Identity = 198/514 (38.52%), Postives = 302/514 (58.75%), Query Frame = 0
Query: 44 VSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 103
+S+F+ V+ +++ +SR D +P P GALP+ GHLHLL K+ L
Sbjct: 10 LSLFSLALVIFGYIFLRKQLSR-CEVDSSTIPEPL---GALPLFGHLHLLRGKKLLCKKL 69
Query: 104 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 163
A M+ +GP+F+L+ G + +V S + ++ FTTND A+RP + + +GY+ A
Sbjct: 70 AAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLT 129
Query: 164 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKK 223
L+PYG +WR+ R++ T+ L +NH +E L H+R EV IK LYK + ++V++
Sbjct: 130 LAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK---GNGGTSIVKIDM 189
Query: 224 WFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLG 283
F LT + I R +VGKR S K+AL+ L I + D P+L WLD
Sbjct: 190 LFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFA 249
Query: 284 GHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDAN 343
+ + MK K LD K+L +H + + ++ ++ M++++ + + + +
Sbjct: 250 KNSQ-MKRLFKELDSVNTKWLHEHLKKRS-RNEKDQERTIMDLLLDILPEDIVISGHVRD 309
Query: 344 TVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKN 403
++KAT L + L G D+T++T+TW +SLLLNN L+ AQ E+D VG+GR ++ESD++N
Sbjct: 310 VIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQN 369
Query: 404 LLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDS 463
L YLQAIVKET RLYP APL E+ EDC GY + GTRL+VN+ KL RDP +W D
Sbjct: 370 LKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDP 429
Query: 464 CEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRP 523
F+PERF+ + + + + IPF +GRR CPG++ L+V+H LA LL GF++ +
Sbjct: 430 KTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKV 489
Query: 524 SEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
S+EPLDM E GL + P+EVV+ P L ++Y
Sbjct: 490 SDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9SZ46 | 5.0e-142 | 49.53 | Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... | [more] |
| O49394 | 5.0e-134 | 47.83 | Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... | [more] |
| A0A0N7F297 | 5.6e-133 | 48.54 | Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... | [more] |
| O49858 | 9.5e-133 | 48.33 | Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1 | [more] |
| O49859 | 1.4e-128 | 46.24 | Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1C9U5 | 0.0 | 100.00 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=... | [more] |
| A0A6J1CCZ4 | 0.0 | 85.88 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=... | [more] |
| A0A6J1FQP6 | 8.45e-274 | 74.46 | cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| A0A6J1FPG1 | 8.45e-274 | 74.46 | cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| A0A6J1IFL6 | 5.06e-270 | 71.46 | cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G31940.1 | 3.6e-143 | 49.53 | cytochrome P450, family 82, subfamily C, polypeptide 4 | [more] |
| AT4G31970.1 | 3.6e-135 | 47.83 | cytochrome P450, family 82, subfamily C, polypeptide 2 | [more] |
| AT4G31950.1 | 9.4e-128 | 46.53 | cytochrome P450, family 82, subfamily C, polypeptide 3 | [more] |
| AT2G25160.1 | 1.1e-99 | 39.04 | cytochrome P450, family 82, subfamily F, polypeptide 1 | [more] |
| AT3G25180.1 | 5.0e-97 | 38.52 | cytochrome P450, family 82, subfamily G, polypeptide 1 | [more] |
