MC11g_new0035 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 5.0e-142
Identity = 264/533 (49.53%), Postives = 363/533 (68.11%), Query Frame = 0

Query: 39  MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
           M+    S+F  I V +F   ALF  S+K      +    P   GA P+IGHLHLL  KE 
Sbjct: 1   MDTSLFSLFVPILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60

Query: 99  -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
             + TL KMAD YGP  +L+ G  +A VVSS+E+A++ FT ND+  ASRP   A+K +GY
Sbjct: 61  LLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGY 120

Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKS--EE 218
           ++A+FG +PY   WR+ R++AT+ELL+N RL+ L+HVR+ E+   +K+LY L  K+   +
Sbjct: 121 NFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK 180

Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGS---------EECKKALRDFFELFG 278
             +V++K W  D+TL+ I R+V GKR+   F G GS          +CKKA+  FF L G
Sbjct: 181 PVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIG 240

Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
           IF  SD+FP LS+ DL GHEK MK T   LD   ++++++H Q   F+ ++E D DF++V
Sbjct: 241 IFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDV 300

Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
           M+S A + +     YDANT IK+TCL +ILGG DT+  T+TW ISLLLNN+E L+KAQ E
Sbjct: 301 MMSLAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDE 360

Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
           +D  VGR R V++SD++NL+YLQAI+KETLRLYPA PLL P E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTR 420

Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
           LIVNV K+QRDP V+ +  EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAM 480

Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           QV+HL LA  LH F +    + P+DM E+ GLT  +  PLEV+++P +  +++
Sbjct: 481 QVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 5.0e-134
Identity = 253/529 (47.83%), Postives = 355/529 (67.11%), Query Frame = 0

Query: 39  MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
           M+    S+F  I V +F   ALF  S+K      + P P    GA P+IGHLHLLS KE 
Sbjct: 1   MDTSLFSLFVPILVFVFI--ALFKKSKK--PKHVKAPAP---SGAWPIIGHLHLLSGKEQ 60

Query: 99  -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
             + TL KMAD YGP  +LR G  +  VVSS+E+A++ FT ND+  ASRP   A+K +GY
Sbjct: 61  LLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGY 120

Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
           D A+FG +PY   WR+ R++ATLELL+N RL+ L+HVR+ E+   +++LY L VK    E
Sbjct: 121 DCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE 180

Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRF--SAAFNGSGSEE---CKKALRDFFELFGIFIP 278
             +V++K W  D++L+ + R+V GKR+    + +   +EE   C+K + +FF L GIF  
Sbjct: 181 PVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 240

Query: 279 SDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMIS- 338
           SD+FP L W D  GHEK MK T + LD   ++++++H Q    + ++  D DF++VM+S 
Sbjct: 241 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 300

Query: 339 AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQ 398
           A + +     +DA T IK+TCL +ILGG +T+  T+TW ISLLLNN++ L+KAQ E+D  
Sbjct: 301 AEQGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 360

Query: 399 VGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVN 458
           VGR R V++SD++NL+Y+QAI+KETLRLYPA PLL   E+IEDCT AGY++  GTR++VN
Sbjct: 361 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 420

Query: 459 VQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFALQVIH 518
           V K+QRDP V+ +  EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+QV+H
Sbjct: 421 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 480

Query: 519 LTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           L LA  L  F +    + P+DM ES GLT  +  PLE++++P L   +Y
Sbjct: 481 LGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 5.6e-133
Identity = 249/513 (48.54%), Postives = 336/513 (65.50%), Query Frame = 0

Query: 45  SIFTGIFVLL-FFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 104
           ++  G FVLL  F Y ++           ++  PP+  GA P+IGHLHLL+  +  H  L
Sbjct: 9   TLLLGFFVLLPCFFYFVWK------KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKIL 68

Query: 105 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 164
           +  AD  GP+F ++ G+ +ALVV++ EIA+E FTTNDR F +RP  VA+K++GY+Y M G
Sbjct: 69  SSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLG 128

Query: 165 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLC-VKSEEKAVVEMK 224
           ++PYGP+WR  R++  LE L+N RL+ L+HV   E+    KELYKL   ++ +  +V+MK
Sbjct: 129 VAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMK 188

Query: 225 KWFGDLTLDTIFRIVVGKRFSAAFNGSGSEE-----CKKALRDFFELFGIFIPSDSFPFL 284
           +W  DLTL+   ++VVGKRF  + + S  EE     C K LR+ F L G F+ SD  P+L
Sbjct: 189 QWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYL 248

Query: 285 SWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGES 344
            WLDLGGHEK MK T K LD  F  +L +H +       E+ DQDFM+VM+S +  E   
Sbjct: 249 RWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSIL--EESK 308

Query: 345 FSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVK 404
              D +T+ K  CL +ILGG DTT  T+TW +SLLLNN   L+KAQ ELD  VGR R V 
Sbjct: 309 LGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVD 368

Query: 405 ESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDP 464
           ESD+  L Y+ AI+KETLRLYP  PLL P    EDCT AGYH+  GTRLIVN  K+QRDP
Sbjct: 369 ESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDP 428

Query: 465 LVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHG 524
           LVW    E++PERFL  +++ D++GQ  +LIPF +GRR CP IS ALQV+ LTLA++LHG
Sbjct: 429 LVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHG 488

Query: 525 FKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTP 551
           F++  P++  +DM E+ G+   +  PLEV++ P
Sbjct: 489 FELRTPNQNKVDMTETPGIVHAKATPLEVLVAP 511

BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 9.5e-133
Identity = 232/480 (48.33%), Postives = 328/480 (68.33%), Query Frame = 0

Query: 78  PEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFT 137
           P   GA P++GHL LL+  +  H  L  +AD YGP+FT++ GMK ALV+S+WE+++E FT
Sbjct: 39  PVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFT 98

Query: 138 TNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRIL 197
           TND   +SRPKLVA +++ Y+ A  GL+PYGP+WR+ R++ T E L+N R+E+  H+R+ 
Sbjct: 99  TNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVS 158

Query: 198 EVKNWIKELYKLCV---KSEEK-AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEE 257
           EV+  IKEL+ +     K+E +  +V++K+W   LT + + R+VVGKR+    +  G ++
Sbjct: 159 EVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDK 218

Query: 258 CK---KALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQT 317
            +   K +R+F  L G F  +D  P L WLDLGGHEKAMK  AK +D    ++L++H Q 
Sbjct: 219 AQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQK 278

Query: 318 TNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVIS 377
                + E+D+DFM+VMISA+ N  +  ++DA+T+ KAT L +ILGG D+T VT+TW +S
Sbjct: 279 KLLGENVESDRDFMDVMISAL-NGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALS 338

Query: 378 LLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESI 437
           LLL N   L KA+ E+D Q+G+   ++ESD+  L+YLQAIVKETLRLYP AP   P E  
Sbjct: 339 LLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFT 398

Query: 438 EDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPF 497
           E+C   GYHI  GTRLI N+ K+ RDP VW D  EF+PERFLT+ K+ D+RG + +L+PF
Sbjct: 399 ENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPF 458

Query: 498 RNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTP 551
            +GRR+C G+S  L ++H TLANLLH F I  PS EP+DM E  G T+ +  PLE+++ P
Sbjct: 459 GSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKP 517

BLAST of MC11g_new0035 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.4e-128
Identity = 240/519 (46.24%), Postives = 340/519 (65.51%), Query Frame = 0

Query: 44  VSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 103
           + + + IF+L  FLY    + +  G+ +      P  GGA P+ GHL LL   +  H  L
Sbjct: 13  IGVVSLIFLLCLFLYG--PLKKVHGSSKE----APTVGGAWPIFGHLPLLIGSKSPHKAL 72

Query: 104 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 163
             +A+ +GP+FT++ G KKALVVS WE+ARE FTTND   ++RPKL+ ++L+ Y+ AM  
Sbjct: 73  GALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLL 132

Query: 164 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKL--CVKSE-EKAVVE 223
           ++PYGP+WR+ R++   E+L++ R+E+LQ VR+ EV+N I ELY +    K+E + A VE
Sbjct: 133 VAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVE 192

Query: 224 MKKWFGDLTLDTIFRIVVGKRF-SAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSW 283
           +K+WF     + + R+VVGKRF SA      +E+C KA+ +F  L G+F   D+ P+L W
Sbjct: 193 LKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRW 252

Query: 284 LDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESF- 343
           LD GG+EKAMK TAK LD    ++L++H Q        +  QDFM VM+S++  +G++  
Sbjct: 253 LDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSL--DGKTID 312

Query: 344 SYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKE 403
             DA+T+IK+T L +I  G + +  T+ W + L+L N   L+  + ELD QVG+ R + E
Sbjct: 313 GIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICE 372

Query: 404 SDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPL 463
           SD+ NL+YLQA+VKETLRLY   PL  P E  EDCT  GYH+  GTRLI N+ K+  DP 
Sbjct: 373 SDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPN 432

Query: 464 VWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGF 523
           VW D  EF+P+RFLT+ K+ DV+G   QL+PF +GRR+CPGISF LQ +HL LA+ LH F
Sbjct: 433 VWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSF 492

Query: 524 KIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           +I  PS EPLDM E+ G+T+ +  PLEV++ PCL    Y
Sbjct: 493 EILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522

BLAST of MC11g_new0035 vs. NCBI nr
Match: XP_022138580.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 1147 bits (2967), Expect = 0.0
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
           MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL
Sbjct: 1   MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60

Query: 61  FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
           FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM
Sbjct: 61  FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120

Query: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
           KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL
Sbjct: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180

Query: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
           ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK
Sbjct: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240

Query: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
           RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF
Sbjct: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300

Query: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
           DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT
Sbjct: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360

Query: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
           TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA
Sbjct: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420

Query: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
           APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV
Sbjct: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480

Query: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
           RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR
Sbjct: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540

Query: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
           KAPLEVVLTPCLPAQVYGKVCNKIMNKK
Sbjct: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568

BLAST of MC11g_new0035 vs. NCBI nr
Match: XP_022138438.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0

Query: 39  MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98
           MEV  HV  IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60

Query: 99  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120

Query: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180

Query: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278
           AVVEMK WFGD+TL+TIFR+VVGKRFS AF+  +GSEECKKALRDFF LFGIFIPSD+F 
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240

Query: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338
           FLSW DLGGHEKAMK TA+LLD  FDKFLK+H Q   +NF  SE  +QDFM+VMIS VKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300

Query: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398
           E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR 
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360

Query: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458
           R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420

Query: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518
           QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480

Query: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524

BLAST of MC11g_new0035 vs. NCBI nr
Match: XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 770 bits (1988), Expect = 1.91e-275
Identity = 387/521 (74.28%), Postives = 438/521 (84.07%), Query Frame = 0

Query: 41  VPHVSIFTGI-FVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPT 100
           + H+    GI F  L FLY  F  SR+S A R+R  +PPEAGGA PVIGHLHLL A EPT
Sbjct: 1   MEHLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKR--IPPEAGGAWPVIGHLHLLHAGEPT 60

Query: 101 HITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDY 160
           HITLAKMADAYGP+FT RFGMKKAL+VSSWEIA+EFFTTNDR+FASRPKLVASKLL YDY
Sbjct: 61  HITLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDY 120

Query: 161 AMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEE---K 220
           AM G SPY PHWRQ R++ATLELLTNHRLE+LQH+R  EV+ W+ EL+K+ +KS E   K
Sbjct: 121 AMIGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSIKSNERGEK 180

Query: 221 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPF 280
             VEMKKW  D+TL+TIF++V+GKRFSA  +G+  E   KAL DFF LF IF+PSDSFPF
Sbjct: 181 VAVEMKKWLADITLNTIFKMVIGKRFSAIQHGN--ENFPKALIDFFGLFHIFVPSDSFPF 240

Query: 281 LSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGE 340
           LSWLDLGGHEK MK TAK++D AF KFL+ H +  N A +E A+QDFM+VMIS V+ +G+
Sbjct: 241 LSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNDAEAETAEQDFMDVMISRVEEDGQ 300

Query: 341 SFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREV 400
            FSYDA+TVIKAT LNM+L GFDTTT+TM W +SLLLNN+  L+ AQLELDE+VGR R+V
Sbjct: 301 HFSYDADTVIKATSLNMVLAGFDTTTITMVWALSLLLNNEGVLENAQLELDEKVGRSRQV 360

Query: 401 KESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRD 460
           KESDVKNL YL+AIVKETLRLYPAAPLLVPHESIEDCTAAGYHI  GTRLIVNVQKLQ+D
Sbjct: 361 KESDVKNLPYLRAIVKETLRLYPAAPLLVPHESIEDCTAAGYHISKGTRLIVNVQKLQKD 420

Query: 461 PLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLH 520
           PLVWE  CEFRPERFLTSQK+FDVRGQSPQ IPF NGRRMCP ISFALQ+IHLTLAN LH
Sbjct: 421 PLVWEGPCEFRPERFLTSQKDFDVRGQSPQFIPFGNGRRMCPAISFALQMIHLTLANFLH 480

Query: 521 GFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           GFKI+R S+EPLDMEESVGLTS+RKAPLEVVLTP LPA VY
Sbjct: 481 GFKIERSSDEPLDMEESVGLTSLRKAPLEVVLTPRLPAHVY 517

BLAST of MC11g_new0035 vs. NCBI nr
Match: XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 765 bits (1975), Expect = 1.75e-273
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 50  IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
           IF LLFFLYALF +SR+S A R++LP  PE GGA PVIGHLHLLS KEPTHITL+K+AD 
Sbjct: 10  IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69

Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
           YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70  YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129

Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
           HWR AR++ATLELLTNHRLE+LQH+R  EV+ W  +LY+L VK  +EK VVEMK  F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189

Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
           TL+TIF +V+GKRFS AF+G   E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K 
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249

Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
           MK TAK+LD AF+KFLK+H Q  +    E  ++DFM+VMIS V  +G  FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309

Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
           TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369

Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
           AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP  WE+  EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429

Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
           +RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489

Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0035 vs. NCBI nr
Match: XP_022940485.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 765 bits (1975), Expect = 1.75e-273
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 50  IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
           IF LLFFLYALF +SR+S A R++LP  PE GGA PVIGHLHLLS KEPTHITL+K+AD 
Sbjct: 10  IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69

Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
           YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70  YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129

Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
           HWR AR++ATLELLTNHRLE+LQH+R  EV+ W  +LY+L VK  +EK VVEMK  F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189

Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
           TL+TIF +V+GKRFS AF+G   E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K 
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249

Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
           MK TAK+LD AF+KFLK+H Q  +    E  ++DFM+VMIS V  +G  FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309

Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
           TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369

Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
           AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP  WE+  EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429

Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
           +RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489

Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match: A0A6J1C9U5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=3 SV=1)

HSP 1 Score: 1147 bits (2967), Expect = 0.0
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60
           MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL
Sbjct: 1   MLWISPNRDRFILYKPRWAFGSGHFFSKNVKTYWSNQEMEVPHVSIFTGIFVLLFFLYAL 60

Query: 61  FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120
           FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM
Sbjct: 61  FNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADAYGPMFTLRFGM 120

Query: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180
           KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL
Sbjct: 121 KKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGPHWRQARRVATL 180

Query: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240
           ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK
Sbjct: 181 ELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKKWFGDLTLDTIFRIVVGK 240

Query: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300
           RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF
Sbjct: 241 RFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAF 300

Query: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360
           DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT
Sbjct: 301 DKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKATCLNMILGGFDT 360

Query: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420
           TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA
Sbjct: 361 TTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPA 420

Query: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480
           APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV
Sbjct: 421 APLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRPERFLTSQKNFDV 480

Query: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540
           RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR
Sbjct: 481 RGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVR 540

Query: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568
           KAPLEVVLTPCLPAQVYGKVCNKIMNKK
Sbjct: 541 KAPLEVVLTPCLPAQVYGKVCNKIMNKK 568

BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0

Query: 39  MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98
           MEV  HV  IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60

Query: 99  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120

Query: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180

Query: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278
           AVVEMK WFGD+TL+TIFR+VVGKRFS AF+  +GSEECKKALRDFF LFGIFIPSD+F 
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240

Query: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338
           FLSW DLGGHEKAMK TA+LLD  FDKFLK+H Q   +NF  SE  +QDFM+VMIS VKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300

Query: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398
           E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR 
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360

Query: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458
           R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420

Query: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518
           QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480

Query: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524

BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 8.45e-274
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 50  IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
           IF LLFFLYALF +SR+S A R++LP  PE GGA PVIGHLHLLS KEPTHITL+K+AD 
Sbjct: 10  IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69

Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
           YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70  YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129

Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
           HWR AR++ATLELLTNHRLE+LQH+R  EV+ W  +LY+L VK  +EK VVEMK  F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189

Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
           TL+TIF +V+GKRFS AF+G   E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K 
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249

Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
           MK TAK+LD AF+KFLK+H Q  +    E  ++DFM+VMIS V  +G  FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309

Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
           TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369

Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
           AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP  WE+  EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429

Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
           +RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489

Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 8.45e-274
Identity = 379/509 (74.46%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 50  IFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKMADA 109
           IF LLFFLYALF +SR+S A R++LP  PE GGA PVIGHLHLLS KEPTHITL+K+AD 
Sbjct: 10  IFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITLSKLADK 69

Query: 110 YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSPYGP 169
           YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM GLSPY P
Sbjct: 70  YGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPYSP 129

Query: 170 HWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK-SEEKAVVEMKKWFGDL 229
           HWR AR++ATLELLTNHRLE+LQH+R  EV+ W  +LY+L VK  +EK VVEMK  F D+
Sbjct: 130 HWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMKNLFADI 189

Query: 230 TLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLGGHEKA 289
           TL+TIF +V+GKRFS AF+G   E+C+KALRD FELFG+F+PSDSFPFL WLD GGH+K 
Sbjct: 190 TLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWWLDFGGHQKR 249

Query: 290 MKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDANTVIKA 349
           MK TAK+LD AF+KFLK+H Q  +    E  ++DFM+VMIS V  +G  FS+D +TV+KA
Sbjct: 250 MKKTAKILDGAFEKFLKEHRQRRDRVKLEMKEEDFMDVMISRVNEDGNLFSFDGDTVVKA 309

Query: 350 TCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKNLLYLQ 409
           TCLNMILGGFDT TVTMTW +SLLLNN+E+L+KAQLELDEQVGR R+V+ESDVKNL YLQ
Sbjct: 310 TCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQ 369

Query: 410 AIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDSCEFRP 469
           AIVKETLRLYPAAPLLVPHES+EDCT AGY+IP GTRLIV+ QKLQ+DP  WE+  EFRP
Sbjct: 370 AIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRP 429

Query: 470 ERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRPSEEPL 529
           +RFLTSQKN DVRGQSPQLIPF NGRRMCPGISFALQV+HLTL NLLHGF I R SEEPL
Sbjct: 430 KRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFNIGRMSEEPL 489

Query: 530 DMEESVGLTSVRKAPLEVVLTPCLPAQVY 557
           DMEES GLTSVRKAPLEVVL+P LPA VY
Sbjct: 490 DMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0035 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 756 bits (1951), Expect = 5.06e-270
Identity = 378/529 (71.46%), Postives = 437/529 (82.61%), Query Frame = 0

Query: 41  VPHVSIFTGI-FVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPT 100
           + H+    GI F  LFF+Y LF  SR+S A R+R  +PPEAGGA PVIGHLHLL A +PT
Sbjct: 1   MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKR--IPPEAGGAWPVIGHLHLLHAGDPT 60

Query: 101 HITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDY 160
           HI LAKMADAYGPMFTLRFGMKKALVVSSWEIA+EF TTNDR+FASRPKLVASKLL YDY
Sbjct: 61  HIALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDY 120

Query: 161 AMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEE---K 220
           AM G SPY PHWRQ R++ATLELLTNHRLE+LQH+R+ EV+ W+KEL+K+ ++S E   K
Sbjct: 121 AMMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDK 180

Query: 221 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPF 280
             VEMKKW  D+TL+TIF++V+GKRFSA  +G+  E   KAL DFF LF IF+PSDSFPF
Sbjct: 181 VAVEMKKWLADITLNTIFKMVIGKRFSAIQHGT--ENFPKALIDFFGLFHIFVPSDSFPF 240

Query: 281 LSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGE 340
           LSWLDLGGHEK MK TAK++D AF KFL+ H +  N   +E A+QDFM+VMIS V+ + +
Sbjct: 241 LSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNEGQAETAEQDFMDVMISRVEEDEQ 300

Query: 341 SFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREV 400
            FSYDA+TVIKATCLNMI+GGFDTT VTMTW + LLLNN+E L+K QLELDE+VGR  +V
Sbjct: 301 HFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQV 360

Query: 401 KESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRD 460
           KESDVKNL YLQAIVKETLRLYPA PL+ PHES+EDCT AGYHI  GTRLIVNVQKLQ+D
Sbjct: 361 KESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKD 420

Query: 461 PLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLH 520
           P VWE+  EF PERF+TSQKNFDVRG SPQ IPF NGRRMCP ISFALQV+H+TLAN LH
Sbjct: 421 PRVWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANFLH 480

Query: 521 GFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVYGKVCNKIM 565
           GF+I+RPSEE LDMEE +GLTS+RKAPLEVVLTP LPA +Y    N+I+
Sbjct: 481 GFEIERPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIYESSRNRIL 525

BLAST of MC11g_new0035 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 506.1 bits (1302), Expect = 3.6e-143
Identity = 264/533 (49.53%), Postives = 363/533 (68.11%), Query Frame = 0

Query: 39  MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
           M+    S+F  I V +F   ALF  S+K      +    P   GA P+IGHLHLL  KE 
Sbjct: 1   MDTSLFSLFVPILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60

Query: 99  -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
             + TL KMAD YGP  +L+ G  +A VVSS+E+A++ FT ND+  ASRP   A+K +GY
Sbjct: 61  LLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGY 120

Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKS--EE 218
           ++A+FG +PY   WR+ R++AT+ELL+N RL+ L+HVR+ E+   +K+LY L  K+   +
Sbjct: 121 NFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK 180

Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGS---------EECKKALRDFFELFG 278
             +V++K W  D+TL+ I R+V GKR+   F G GS          +CKKA+  FF L G
Sbjct: 181 PVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIG 240

Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
           IF  SD+FP LS+ DL GHEK MK T   LD   ++++++H Q   F+ ++E D DF++V
Sbjct: 241 IFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDV 300

Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
           M+S A + +     YDANT IK+TCL +ILGG DT+  T+TW ISLLLNN+E L+KAQ E
Sbjct: 301 MMSLAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDE 360

Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
           +D  VGR R V++SD++NL+YLQAI+KETLRLYPA PLL P E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTR 420

Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
           LIVNV K+QRDP V+ +  EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAM 480

Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           QV+HL LA  LH F +    + P+DM E+ GLT  +  PLEV+++P +  +++
Sbjct: 481 QVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of MC11g_new0035 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.6e-135
Identity = 253/529 (47.83%), Postives = 355/529 (67.11%), Query Frame = 0

Query: 39  MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
           M+    S+F  I V +F   ALF  S+K      + P P    GA P+IGHLHLLS KE 
Sbjct: 1   MDTSLFSLFVPILVFVFI--ALFKKSKK--PKHVKAPAP---SGAWPIIGHLHLLSGKEQ 60

Query: 99  -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
             + TL KMAD YGP  +LR G  +  VVSS+E+A++ FT ND+  ASRP   A+K +GY
Sbjct: 61  LLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGY 120

Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
           D A+FG +PY   WR+ R++ATLELL+N RL+ L+HVR+ E+   +++LY L VK    E
Sbjct: 121 DCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE 180

Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRF--SAAFNGSGSEE---CKKALRDFFELFGIFIP 278
             +V++K W  D++L+ + R+V GKR+    + +   +EE   C+K + +FF L GIF  
Sbjct: 181 PVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 240

Query: 279 SDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMIS- 338
           SD+FP L W D  GHEK MK T + LD   ++++++H Q    + ++  D DF++VM+S 
Sbjct: 241 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 300

Query: 339 AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQ 398
           A + +     +DA T IK+TCL +ILGG +T+  T+TW ISLLLNN++ L+KAQ E+D  
Sbjct: 301 AEQGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 360

Query: 399 VGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVN 458
           VGR R V++SD++NL+Y+QAI+KETLRLYPA PLL   E+IEDCT AGY++  GTR++VN
Sbjct: 361 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 420

Query: 459 VQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFALQVIH 518
           V K+QRDP V+ +  EFRPERF+T + K FDVRGQ+ +L+PF +GRR CPG S A+QV+H
Sbjct: 421 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 480

Query: 519 LTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           L LA  L  F +    + P+DM ES GLT  +  PLE++++P L   +Y
Sbjct: 481 LGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of MC11g_new0035 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 454.9 bits (1169), Expect = 9.4e-128
Identity = 248/533 (46.53%), Postives = 343/533 (64.35%), Query Frame = 0

Query: 39  MEVPHVSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEP 98
           M+    S+F  I V +F   ALF  S+K      +    P   GA P+IGHLHLL  KE 
Sbjct: 1   MDTSLFSLFVSILVFVFI--ALFKKSKKP-----KYVKAPAPSGAWPIIGHLHLLGGKEQ 60

Query: 99  -THITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGY 158
             + TL KMAD YGP  +LR G  +  V SS+E+A++ FT ND+  AS     A+K +GY
Sbjct: 61  LLYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY 120

Query: 159 DYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVK--SEE 218
            +           W + R++A +ELL+N RL+ L +VR+ E+   +K+LY L VK    E
Sbjct: 121 VF-----------WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSE 180

Query: 219 KAVVEMKKWFGDLTLDTIFRIVVGKRFSAAFNGSGSE---------ECKKALRDFFELFG 278
             +V++K W  D+  + I R+V GKR+   F G G+E         + +K +  FF L G
Sbjct: 181 PVMVDLKSWLEDMIANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVG 240

Query: 279 IFIPSDSFPFLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEV 338
           IF  SD+FP L WLDL GHEK MK T + LD   ++++++H Q    + ++  D DF++V
Sbjct: 241 IFTVSDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDV 300

Query: 339 MIS-AVKNEGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLE 398
           M+S A + +     YDANT IK TCL +ILGG +T+  T+TW ISLLLNN++ L+K Q E
Sbjct: 301 MLSLAEQGKLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDE 360

Query: 399 LDEQVGRGREVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTR 458
           +D  VGR R V++SD+KNL+YLQAI+KETLRLYPAAPLL   E++EDCT AGY++P GTR
Sbjct: 361 IDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTR 420

Query: 459 LIVNVQKLQRDPLVWEDSCEFRPERFLTSQ-KNFDVRGQSPQLIPFRNGRRMCPGISFAL 518
           LIVNV K+QRDP V+ +  EFRPERF+T + K+FDVRGQ+ +L+PF +GRR CPG S A+
Sbjct: 421 LIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAM 480

Query: 519 QVIHLTLANLLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           Q++HL LA  LH F++    + P+DM ES GLT  +  PLEV++ P L  +++
Sbjct: 481 QMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

BLAST of MC11g_new0035 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-99
Identity = 203/520 (39.04%), Postives = 302/520 (58.08%), Query Frame = 0

Query: 52  VLLFFLYALF-----NMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITLAKM 111
           ++LF L ALF      + +     + +    P   GA P++GHLHL     PTH+T   M
Sbjct: 4   IMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAM 63

Query: 112 ADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFGLSP 171
           AD YGP+F  + G  K ++++S E+A+E +T +D+L   RP+L ASKLLGY+ +    SP
Sbjct: 64  ADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSP 123

Query: 172 YGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELY---KLCVKSEEKAVVEMKK 231
           YG +WR+ R++A  EL +   ++     R  E     + LY   +   K +E  +V+MK+
Sbjct: 124 YGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQ 183

Query: 232 WFGDLTLDTIFRIVVGKRFSAAFNGSGSEE---CKKALRDFFELFGIFIPSDSFPFLSWL 291
            F DLT +    +V GKR+         +E   C K +R+F + F +F+ SD  P L +L
Sbjct: 184 EFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFL 243

Query: 292 DLGGHEKAMKHTAKLLDHAFDKFLKDH-NQTTNFAASEEADQDFMEVMISAVKNEGESFS 351
           D    ++ MK TAK LD   + ++++H N+ ++   SE    D+++++I  +  +     
Sbjct: 244 D-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSE---NDYLDILIKILGQDKIPGL 303

Query: 352 YDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKES 411
            D +T IKA CLN++L G +T  V + W +SLLLNN   L+KAQ ELD ++G+ R V+E 
Sbjct: 304 SDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEEL 363

Query: 412 DVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAA--GYHIPIGTRLIVNVQKLQRDP 471
           D+K+L+YLQAIVKET RLYP  PL+     +ED   A    H+P GT+L+V+  K+ RDP
Sbjct: 364 DIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDP 423

Query: 472 LVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHG 531
            VW +  +F PERFLTS +  DV GQS +  PF  GRR CP I   ++++H  L   LH 
Sbjct: 424 NVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHS 483

Query: 532 FKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           F + RPS + +DM ES GL + +  PLEV + P L   +Y
Sbjct: 484 FDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLY 518

BLAST of MC11g_new0035 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 352.8 bits (904), Expect = 5.0e-97
Identity = 198/514 (38.52%), Postives = 302/514 (58.75%), Query Frame = 0

Query: 44  VSIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAKEPTHITL 103
           +S+F+   V+  +++    +SR    D   +P P    GALP+ GHLHLL  K+     L
Sbjct: 10  LSLFSLALVIFGYIFLRKQLSR-CEVDSSTIPEPL---GALPLFGHLHLLRGKKLLCKKL 69

Query: 104 AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLGYDYAMFG 163
           A M+  +GP+F+L+ G  + +V S  +  ++ FTTND   A+RP +   + +GY+ A   
Sbjct: 70  AAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLT 129

Query: 164 LSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEKAVVEMKK 223
           L+PYG +WR+ R++ T+ L +NH +E L H+R  EV   IK LYK    +   ++V++  
Sbjct: 130 LAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK---GNGGTSIVKIDM 189

Query: 224 WFGDLTLDTIFRIVVGKRFSAAFNGSGSEECKKALRDFFELFGIFIPSDSFPFLSWLDLG 283
            F  LT + I R +VGKR       S     K+AL+    L  I +  D  P+L WLD  
Sbjct: 190 LFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFA 249

Query: 284 GHEKAMKHTAKLLDHAFDKFLKDHNQTTNFAASEEADQDFMEVMISAVKNEGESFSYDAN 343
            + + MK   K LD    K+L +H +  +    ++ ++  M++++  +  +     +  +
Sbjct: 250 KNSQ-MKRLFKELDSVNTKWLHEHLKKRS-RNEKDQERTIMDLLLDILPEDIVISGHVRD 309

Query: 344 TVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRGREVKESDVKN 403
            ++KAT L + L G D+T++T+TW +SLLLNN   L+ AQ E+D  VG+GR ++ESD++N
Sbjct: 310 VIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQN 369

Query: 404 LLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKLQRDPLVWEDS 463
           L YLQAIVKET RLYP APL    E+ EDC   GY +  GTRL+VN+ KL RDP +W D 
Sbjct: 370 LKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDP 429

Query: 464 CEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLANLLHGFKIDRP 523
             F+PERF+  +   +    + + IPF +GRR CPG++  L+V+H  LA LL GF++ + 
Sbjct: 430 KTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKV 489

Query: 524 SEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 558
           S+EPLDM E  GL   +  P+EVV+ P L  ++Y
Sbjct: 490 SDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512