MC11g1265 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g1265 vs. ExPASy Swiss-Prot
Match: Q9ZRV4 (DNA mismatch repair protein MLH1 OS=Arabidopsis thaliana OX=3702 GN=MLH1 PE=2 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 1.2e-305
Identity = 538/738 (72.90%), Postives = 621/738 (84.15%), Query Frame = 0

Query: 6   DDEIVPMETDGEEEFPR-----KEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 65
           DD  +  E + EEE P      +EPPKI RL ESVVNRIAAGEVIQRPVSA+KELVENSL
Sbjct: 3   DDSSLTAEME-EEESPATTIVPREPPKIQRLEESVVNRIAAGEVIQRPVSAVKELVENSL 62

Query: 66  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 125
           DA S+SI+VVVKDGGLKLIQVSDDGHGIR EDLPILCERHTTSKL+KFEDL S+ SMGFR
Sbjct: 63  DADSSSISVVVKDGGLKLIQVSDDGHGIRREDLPILCERHTTSKLTKFEDLFSLSSMGFR 122

Query: 126 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 185
           GEALASMTYV HVTVTTITKGQ++GYRVSYRDGVMEHEPK CAAVKGTQI VENLFYNM 
Sbjct: 123 GEALASMTYVAHVTVTTITKGQIHGYRVSYRDGVMEHEPKACAAVKGTQIMVENLFYNMI 182

Query: 186 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 245
           ARRKTLQNS+DDY KIVDLLSR AIH+ N SFSCRKHGA +ADVHSVVS SRLD+IR+VY
Sbjct: 183 ARRKTLQNSADDYGKIVDLLSRMAIHYNNVSFSCRKHGAVKADVHSVVSPSRLDSIRSVY 242

Query: 246 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 305
           G SVA+NLMK+EVS  D     F M+G ISNSNYVAKK  +VLFIN R+VECS+LKRA+E
Sbjct: 243 GVSVAKNLMKVEVSSCDSSGCTFDMEGFISNSNYVAKKTILVLFINDRLVECSALKRAIE 302

Query: 306 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 365
           IVYAATLPKASKPFVYMSI LP EHVD+N+HPTKKEV+LLNQE+IIE IQS VE KLR++
Sbjct: 303 IVYAATLPKASKPFVYMSINLPREHVDINIHPTKKEVSLLNQEIIIEMIQSEVEVKLRNA 362

Query: 366 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 425
           NDTRTFQEQ VE   +      +D   +   SG K+QKVPV+KMVRTDS+DPAGRLHA++
Sbjct: 363 NDTRTFQEQKVEYIQSTLTSQKSDSPVSQKPSGQKTQKVPVNKMVRTDSSDPAGRLHAFL 422

Query: 426 QMKPPGLPE--SSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIY 485
           Q KP  LP+  SSL+ VRS VR+RRNPKE A+L+S+Q+L+  +D  CH G+L  VR+C Y
Sbjct: 423 QPKPQSLPDKVSSLSVVRSSVRQRRNPKETADLSSVQELIAGVDSCCHPGMLETVRNCTY 482

Query: 486 VGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILAL 545
           VGMADDVFAL+Q++THLYLANVVNLSKELMYQQ L RFAHFNAIQLS+PAPLSELI+LAL
Sbjct: 483 VGMADDVFALVQYNTHLYLANVVNLSKELMYQQTLRRFAHFNAIQLSDPAPLSELILLAL 542

Query: 546 KEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTP 605
           KEED++P  +  D  KE++AEM ++LLK+KAEMLEE+F +HID + NL+RLP++LDQYTP
Sbjct: 543 KEEDLDPGNDTKDDLKERIAEMNTELLKEKAEMLEEYFSVHIDSSANLSRLPVILDQYTP 602

Query: 606 DMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSS 665
           DMDRVPEF++ L ND++WEDEK+C Q VSAAIGNFYAMHPPLLPNPSGDG+QFY + +  
Sbjct: 603 DMDRVPEFLLCLGNDVEWEDEKSCFQGVSAAIGNFYAMHPPLLPNPSGDGIQFYSK-RGE 662

Query: 666 GKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENG 725
              E  D+ +G V ME+ ++  LLS+AE  WAQREWSIQHVLFPSM+LF KPP S+A NG
Sbjct: 663 SSQEKSDL-EGNVDMEDNLDQDLLSDAENAWAQREWSIQHVLFPSMRLFLKPPASMASNG 722

Query: 726 TLVRVASLEKLYKIFERC 737
           T V+VASLEKLYKIFERC
Sbjct: 723 TFVKVASLEKLYKIFERC 737

BLAST of MC11g1265 vs. ExPASy Swiss-Prot
Match: P40692 (DNA mismatch repair protein Mlh1 OS=Homo sapiens OX=9606 GN=MLH1 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 5.5e-149
Identity = 317/787 (40.28%), Postives = 464/787 (58.96%), Query Frame = 0

Query: 28  ILRLHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVVKDGGLKLIQVSDDGHG 87
           I RL E+VVNRIAAGEVIQRP +AIKE++EN LDA+STSI V+VK+GGLKLIQ+ D+G G
Sbjct: 8   IRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTG 67

Query: 88  IRFEDLPILCERHTTSKLSKFEDLQSIKSMGFRGEALASMTYVGHVTVTTITKGQLYGYR 147
           IR EDL I+CER TTSKL  FEDL SI + GFRGEALAS+++V HVT+TT T      YR
Sbjct: 68  IRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYR 127

Query: 148 VSYRDGVMEHEPKPCAAVKGTQITVENLFYNMTARRKTLQNSSDDYTKIVDLLSRFAIHH 207
            SY DG ++  PKPCA  +GTQITVE+LFYN+  RRK L+N S++Y KI++++ R+++H+
Sbjct: 128 ASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHN 187

Query: 208 TNTSFSCRKHGAARADVHSVVSSSRLDAIRTVYGASVARNLMKIEVSGNDEVCSDFKMDG 267
              SFS +K G   ADV ++ ++S +D IR+++G +V+R L++I   G ++    FKM+G
Sbjct: 188 AGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEI---GCEDKTLAFKMNG 247

Query: 268 LISNSNYVAKKITMVLFINGRMVECSSLKRAVEIVYAATLPKASKPFVYMSIILPPEHVD 327
            ISN+NY  KK   +LFIN R+VE +SL++A+E VYAA LPK + PF+Y+S+ + P++VD
Sbjct: 248 YISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVD 307

Query: 328 VNVHPTKKEVTLLNQEVIIERIQSAVESKLRSSNDTRTFQEQDVESSAA---YQMVMSND 387
           VNVHPTK EV  L++E I+ER+Q  +ESKL  SN +R +  Q +    A    +MV S  
Sbjct: 308 VNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTT 367

Query: 388 YNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYVQ-------MKPPGLPESSLTAVRS 447
              +SS SGS S KV  H+MVRTDS +   +L A++Q        +P  +     T + S
Sbjct: 368 SLTSSSTSGS-SDKVYAHQMVRTDSREQ--KLDAFLQPLSKPLSSQPQAIVTEDKTDISS 427

Query: 448 FVRRRRN--------PKEVA---------------------------------------- 507
              R+++        P EVA                                        
Sbjct: 428 GRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEM 487

Query: 508 --------------------NLTSIQDLVTEIDKNCHAGLLNIVRHCIYVGMADDVFALL 567
                               NLTS+  L  EI++  H  L  ++ +  +VG  +  +AL 
Sbjct: 488 VEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALA 547

Query: 568 QHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKEEDVNPVCNE 627
           QH T LYL N   LS+EL YQ +++ FA+F  ++LS PAPL +L +LAL   +      E
Sbjct: 548 QHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESG--WTE 607

Query: 628 NDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDMDRVPEFIVS 687
            DG KE +AE   + LK+KAEML ++F + ID+ GNL  LPL++D Y P ++ +P FI+ 
Sbjct: 608 EDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILR 667

Query: 688 LANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGKPEDEDIGDG 737
           LA +++W++EK C +S+S     FY++    +                     +E    G
Sbjct: 668 LATEVNWDEEKECFESLSKECAMFYSIRKQYI--------------------SEESTLSG 727

BLAST of MC11g1265 vs. ExPASy Swiss-Prot
Match: Q9JK91 (DNA mismatch repair protein Mlh1 OS=Mus musculus OX=10090 GN=Mlh1 PE=1 SV=2)

HSP 1 Score: 518.8 bits (1335), Expect = 9.7e-146
Identity = 312/791 (39.44%), Postives = 457/791 (57.77%), Query Frame = 0

Query: 28  ILRLHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVVKDGGLKLIQVSDDGHG 87
           I RL E+VVNRIAAGEVIQRP +AIKE++EN LDA+ST+I VVVK+GGLKLIQ+ D+G G
Sbjct: 8   IRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTNIQVVVKEGGLKLIQIQDNGTG 67

Query: 88  IRFEDLPILCERHTTSKLSKFEDLQSIKSMGFRGEALASMTYVGHVTVTTITKGQLYGYR 147
           IR EDL I+CER TTSKL  FEDL SI + GFRGEALAS+++V HVT+TT T      YR
Sbjct: 68  IRKEDLDIVCERFTTSKLQTFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYR 127

Query: 148 VSYRDGVMEHEPKPCAAVKGTQITVENLFYNMTARRKTLQNSSDDYTKIVDLLSRFAIHH 207
            SY DG ++  PKPCA  +GT ITVE+LFYN+  RRK L+N S++Y KI++++ R++IH+
Sbjct: 128 ASYSDGKLQAPPKPCAGNQGTLITVEDLFYNIITRRKALKNPSEEYGKILEVVGRYSIHN 187

Query: 208 TNTSFSCRKHGAARADVHSVVSSSRLDAIRTVYGASVARNLMKIEVSGNDEVCSDFKMDG 267
           +  SFS +K G   +DV ++ +++ +D IR+++G +V+R L  IEV   D+  + FKM+G
Sbjct: 188 SGISFSVKKQGETVSDVRTLPNATTVDNIRSIFGNAVSREL--IEVGCEDKTLA-FKMNG 247

Query: 268 LISNSNYVAKKITMVLFINGRMVECSSLKRAVEIVYAATLPKASKPFVYMSIILPPEHVD 327
            ISN+NY  KK   +LFIN R+VE ++L++A+E VYAA LPK + PF+Y+S+ + P++VD
Sbjct: 248 YISNANYSVKKCIFLLFINHRLVESAALRKAIETVYAAYLPKNTHPFLYLSLEISPQNVD 307

Query: 328 VNVHPTKKEVTLLNQEVIIERIQSAVESKLRSSNDTRTFQEQDVESSAA---YQMVMSND 387
           VNVHPTK EV  L++E I++R+Q  +ESKL  SN +R +  Q +    A    +      
Sbjct: 308 VNVHPTKHEVHFLHEESILQRVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEAARPTT 367

Query: 388 YNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYVQ------------------MKPPG 447
              +SS SGS   KV  ++MVRTDS +   +L A++Q                   +  G
Sbjct: 368 GVASSSTSGS-GDKVYAYQMVRTDSREQ--KLDAFLQPVSSLGPSQPQDPAPVRGARTEG 427

Query: 448 LPE---------------------------------------SSLTAVRSFVRRRRNP-- 507
            PE                                       ++ T+     R+R     
Sbjct: 428 SPERATREDEEMLALPAPAEAAAESENLERESLMETSDAAQKAAPTSSPGSSRKRHREDS 487

Query: 508 --------------------KEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVGMADDV 567
                               + + NLTS+  L  EI + CH  L  ++R+  +VG  +  
Sbjct: 488 DVEMVENASGKEMTAACYPRRRIINLTSVLSLQEEISERCHETLREMLRNHSFVGCVNPQ 547

Query: 568 FALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKEEDVNP 627
           +AL QH T LYL N   LS+EL YQ +++ FA+F  ++LS PAPL +L +LAL   +   
Sbjct: 548 WALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESG- 607

Query: 628 VCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDMDRVPE 687
              E+DG KE +AE   + LK+KAEML ++F + ID+ GNL  LPL++D Y P ++ +P 
Sbjct: 608 -WTEDDGPKEGLAEYIVEFLKKKAEMLADYFSVEIDEEGNLIGLPLLIDSYVPPLEGLPI 667

Query: 688 FIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGKPEDED 737
           FI+ LA +++W++EK C +S+S     FY++    +   S    Q      S+ KP    
Sbjct: 668 FILRLATEVNWDEEKECFESLSKECAMFYSIRKQYILEESTLSGQQSDMPGSTSKP---- 727

BLAST of MC11g1265 vs. ExPASy Swiss-Prot
Match: P97679 (DNA mismatch repair protein Mlh1 OS=Rattus norvegicus OX=10116 GN=Mlh1 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 3.2e-141
Identity = 308/787 (39.14%), Postives = 447/787 (56.80%), Query Frame = 0

Query: 28  ILRLHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVVKDGGLKLIQVSDDGHG 87
           I RL E+VVNRIAAGEVIQRP +AIKE+ EN LDA+ST+I V+V++GGLKLIQ+ D+G G
Sbjct: 8   IRRLDETVVNRIAAGEVIQRPANAIKEMTENCLDAKSTNIQVIVREGGLKLIQIQDNGTG 67

Query: 88  IRFEDLPILCERHTTSKLSKFEDLQSIKSMGFRGEALASMTYVGHVTVTTITKGQLYGYR 147
           IR EDL I+CER TTSKL  FEDL  I + GFRGEALAS+++V HVT+TT T      YR
Sbjct: 68  IRKEDLDIVCERFTTSKLQTFEDLAMISTYGFRGEALASISHVAHVTITTKTADGKCAYR 127

Query: 148 VSYRDGVMEHEPKPCAAVKGTQITVENLFYNMTARRKTLQNSSDDYTKIVDLLSRFAIHH 207
            SY DG ++  PKPCA  +GT ITVE+LFYN+  R+K L+N S++Y KI++++ R++IH+
Sbjct: 128 ASYSDGKLQAPPKPCAGNQGTLITVEDLFYNIITRKKALKNPSEEYGKILEVVGRYSIHN 187

Query: 208 TNTSFSCRKHGAARADVHSVVSSSRLDAIRTVYGASVARNLMKIEVSGNDEVCSDFKMDG 267
           +  SFS +K G   +DV ++ +++ +D IR+++G +V+R L  IEV   D+  + FKM+G
Sbjct: 188 SGISFSVKKQGETVSDVRTLPNATTVDNIRSIFGNAVSREL--IEVGCEDKTLA-FKMNG 247

Query: 268 LISNSNYVAKKITMVLFINGRMVECSSLKRAVEIVYAATLPKASKPFVYMSIILPPEHVD 327
            ISN+NY  KK   +LFIN R+VE ++LK+A+E VYAA LPK + PF+Y+ + + P++VD
Sbjct: 248 YISNANYSVKKCIFLLFINHRLVESAALKKAIEAVYAAYLPKNTHPFLYLILEISPQNVD 307

Query: 328 VNVHPTKKEVTLLNQEVIIERIQSAVESKLRSSNDTRTFQEQDVESSAA---YQMVMSND 387
           VNVHPTK EV  L++E I+ER+Q  +ESKL  SN +R +  Q +    A    + V S  
Sbjct: 308 VNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEAVKSTT 367

Query: 388 YNQNSSQSGSKSQKVPVHKMVRTDSTDP---------AGRLHAYVQMKPPG--------- 447
              +SS SGS   KV  ++MVRTDS D          + RL +  Q   PG         
Sbjct: 368 GIASSSTSGS-GDKVHAYQMVRTDSRDQKLDAFMQPVSRRLPSQPQDPVPGNRTEGSPEK 427

Query: 448 -----------------------LPESSLTAVRSFVRRRRNP------------------ 507
                                  L   S+      V  +R+P                  
Sbjct: 428 AMQKDQEISELPAPMEAAADSASLERESVIGASEVVAPQRHPSSPGSSRKRHPEDSDVEM 487

Query: 508 ----------------KEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVGMADDVFALL 567
                           + + NLTS+  L  EI+   H  L  ++R+  +VG  +  +AL 
Sbjct: 488 MENDSRKEMTAACYPRRRIINLTSVLSLQEEINDRGHETLREMLRNHTFVGCVNPQWALA 547

Query: 568 QHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKEEDVNPVCNE 627
           QH T LYL N   LS+EL YQ +++ FA+F  ++L  PAPL +  +LAL   +      E
Sbjct: 548 QHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLPEPAPLFDFAMLALDSPESG--WTE 607

Query: 628 NDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDMDRVPEFIVS 687
            DG KE +AE   + LK+KA+ML ++F + ID+ GNL  LPL++D Y P ++ +P FI+ 
Sbjct: 608 EDGPKEGLAEYIVEFLKKKAKMLADYFSVEIDEEGNLIGLPLLIDSYVPPLEGLPIFILR 667

Query: 688 LANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGKPEDEDIGDG 737
           LA +++W DE+ C +S+S     FY++    +   S    Q      S  KP        
Sbjct: 668 LATEVNW-DEEECFESLSKECAVFYSIRKQYILEESALSGQQSDMPGSPSKP-------- 727

BLAST of MC11g1265 vs. ExPASy Swiss-Prot
Match: Q54KD8 (DNA mismatch repair protein Mlh1 OS=Dictyostelium discoideum OX=44689 GN=mlh1 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 3.6e-124
Identity = 287/849 (33.80%), Postives = 445/849 (52.41%), Query Frame = 0

Query: 27  KILRLHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVVKDGGLKLIQVSDDGH 86
           KI RL + VVN+I+AGEVIQRP +A+KEL+EN LDA+ST+I V VKDGG+K +Q+ D+G 
Sbjct: 62  KIHRLTQEVVNKISAGEVIQRPSNALKELLENCLDAKSTTITVTVKDGGMKFLQIQDNGS 121

Query: 87  GIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFRGEALASMTYVGHVTVTTITKGQLYGY 146
           GIR ED+ I+CER TTSKL+KFEDL+SI+S GFRGEAL+S+++V H+ + T T      Y
Sbjct: 122 GIRLEDMGIVCERFTTSKLTKFEDLRSIQSFGFRGEALSSISHVSHLKILTKTADSPCAY 181

Query: 147 RVSYRDGVM--------EHEPKPCAAVKGTQITVENLFYNMTARRKTLQNSSDDYTKIVD 206
           R  Y +G +          +PKPCA V GTQITVE+LF+N  +R+  L+N+ D++++IV 
Sbjct: 182 RACYLNGKLTPPSPNEQSSDPKPCAGVNGTQITVEDLFFNTPSRKNVLKNTVDEHSRIVL 241

Query: 207 LLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRL--DAIRTVYGASVARNLMKIEVSGN 266
           L+ ++AI++   SF  +K G    +VH+    + L  D I ++YG  +++ L  I +  N
Sbjct: 242 LMKKYAINNPTVSFILKKQGDPTPEVHTSGGQNSLEKDVIGSLYGTDLSKELKIITIDPN 301

Query: 267 ---------DEVCS-----------DFKMDGLISNSNYVAKKITMVLFINGRMVECSSLK 326
                    D +             DF M G  S++NY +KKI  +LFINGR+V+  +LK
Sbjct: 302 NPNPNNDDDDNISGSQIKNSNLNRLDFTMKGFFSSTNYNSKKINFILFINGRLVDSKNLK 361

Query: 327 RAVEIVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESK 386
             +E VY+  LPK + PF+++ +++PP+++DVN+HPTK EV +L++E IIE IQ  V+ +
Sbjct: 362 TGLEQVYSKYLPKGTHPFMFIRLLVPPKNIDVNIHPTKSEVKILHEEQIIEFIQQKVDQE 421

Query: 387 LRSSNDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGS------------KSQKVPVH-- 446
           L  S++++TF  Q +        V S+   Q +SQS S             S+K P+   
Sbjct: 422 LSISSNSKTFSTQILLPGFDQDNVSSSQKKQKNSQSSSTQTKSTNNNNNPTSRKEPIEYA 481

Query: 447 -KMVRTDSTD------------------------------------PAGRLHAYVQMK-- 506
              +R+DS                                       AG   A  +    
Sbjct: 482 KDKIRSDSKSQTLDAFLNPMDYNNNNDSSIDDNDGSGIGRYDDVDGSAGAAGAGGKFNDN 541

Query: 507 -----------PPGL--PESSLTAVRS--------------------------------- 566
                      P  +  P SS+T ++S                                 
Sbjct: 542 LIFNDNSENEAPRDIDNPLSSVTPIKSKEQRQQQSTTTTTTTTTTTATTKSNSPASKNDI 601

Query: 567 -------FVRRRRNPK-EVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVGMADDVFALL 626
                  F+  R+  K +   LTSI+ L++E   N H GL      C++VG  D  +AL+
Sbjct: 602 KKLQEHTFITPRKTRKYKQVELTSIKTLISEFQSNVHDGLKEFFNDCVFVGCLDHSYALV 661

Query: 627 QHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKEEDVNPVCN- 686
           Q    LYL N+ N++KEL YQ  L RF+ F++I+ S    +  L++++L     +P+   
Sbjct: 662 QFGKKLYLINLENITKELFYQLSLLRFSDFDSIKFSQSLSIYSLLLVSLD----SPLSGW 721

Query: 687 -ENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDMDRVPEFI 737
            E+DG K+K+A+  +KLL  K E+L E+F I I+++G L  +P VLD Y P  D +P F+
Sbjct: 722 MESDGPKDKIADYLTKLLISKKELLNEYFSIEINEDGELVGIPQVLDHYVPCTDNLPIFL 781

BLAST of MC11g1265 vs. NCBI nr
Match: XP_022159006.1 (DNA mismatch repair protein MLH1 [Momordica charantia])

HSP 1 Score: 1439 bits (3725), Expect = 0.0
Identity = 736/736 (100.00%), Postives = 736/736 (100.00%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR
Sbjct: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY
Sbjct: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE
Sbjct: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV
Sbjct: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG
Sbjct: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE
Sbjct: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM
Sbjct: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK
Sbjct: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660

Query: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720
           PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL
Sbjct: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720

Query: 721 VRVASLEKLYKIFERC 736
           VRVASLEKLYKIFERC
Sbjct: 721 VRVASLEKLYKIFERC 736

BLAST of MC11g1265 vs. NCBI nr
Match: XP_022972874.1 (DNA mismatch repair protein MLH1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1299 bits (3362), Expect = 0.0
Identity = 658/736 (89.40%), Postives = 697/736 (94.70%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PMET GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMETAGEEEVPREEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSGSKSQK+PV KMVRTDSTDPAGRLHAYV
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGSKSQKIPVQKMVRTDSTDPAGRLHAYV 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+Y+G
Sbjct: 421 QMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCVYIG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHFNAIQLSNPAPL ELI LAL+E
Sbjct: 481 MADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLALRE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYTPDM
Sbjct: 541 EDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSG 
Sbjct: 601 DRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGN 660

Query: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720
           PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAENG+ 
Sbjct: 661 PEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAENGSF 720

Query: 721 VRVASLEKLYKIFERC 736
           +RVASLEKLYKIFERC
Sbjct: 721 IRVASLEKLYKIFERC 736

BLAST of MC11g1265 vs. NCBI nr
Match: XP_022972873.1 (DNA mismatch repair protein MLH1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 658/739 (89.04%), Postives = 697/739 (94.32%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PMET GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMETAGEEEVPREEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSG---SKSQKVPVHKMVRTDSTDPAGRLH 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSG   SKSQK+PV KMVRTDSTDPAGRLH
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGTEGSKSQKIPVQKMVRTDSTDPAGRLH 420

Query: 421 AYVQMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCI 480
           AYVQMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+
Sbjct: 421 AYVQMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCV 480

Query: 481 YVGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILA 540
           Y+GMADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHFNAIQLSNPAPL ELI LA
Sbjct: 481 YIGMADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLA 540

Query: 541 LKEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYT 600
           L+EEDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYT
Sbjct: 541 LREEDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYT 600

Query: 601 PDMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660
           PDMDRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS
Sbjct: 601 PDMDRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660

Query: 661 SGKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAEN 720
           SG PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAEN
Sbjct: 661 SGNPEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAEN 720

Query: 721 GTLVRVASLEKLYKIFERC 736
           G+ +RVASLEKLYKIFERC
Sbjct: 721 GSFIRVASLEKLYKIFERC 739

BLAST of MC11g1265 vs. NCBI nr
Match: XP_023524451.1 (DNA mismatch repair protein MLH1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1293 bits (3347), Expect = 0.0
Identity = 654/736 (88.86%), Postives = 697/736 (94.70%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PM+T GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMDTGGEEEVPRQEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRF+IHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFSIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSG+KSQKVPV KMVRTDSTDPAGRLHAY+
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGTKSQKVPVQKMVRTDSTDPAGRLHAYL 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+Y+G
Sbjct: 421 QMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCVYIG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHF+AIQLSNPAPL ELI LAL+E
Sbjct: 481 MADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFSAIQLSNPAPLYELISLALRE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYTPDM
Sbjct: 541 EDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSG 
Sbjct: 601 DRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGN 660

Query: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720
           PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAENG+ 
Sbjct: 661 PEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAENGSF 720

Query: 721 VRVASLEKLYKIFERC 736
           +RVASLEKLYKIFERC
Sbjct: 721 IRVASLEKLYKIFERC 736

BLAST of MC11g1265 vs. NCBI nr
Match: XP_023524450.1 (DNA mismatch repair protein MLH1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 654/739 (88.50%), Postives = 697/739 (94.32%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PM+T GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMDTGGEEEVPRQEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRF+IHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFSIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGS---KSQKVPVHKMVRTDSTDPAGRLH 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSG+   KSQKVPV KMVRTDSTDPAGRLH
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGTAGTKSQKVPVQKMVRTDSTDPAGRLH 420

Query: 421 AYVQMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCI 480
           AY+QMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+
Sbjct: 421 AYLQMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCV 480

Query: 481 YVGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILA 540
           Y+GMADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHF+AIQLSNPAPL ELI LA
Sbjct: 481 YIGMADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFSAIQLSNPAPLYELISLA 540

Query: 541 LKEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYT 600
           L+EEDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYT
Sbjct: 541 LREEDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYT 600

Query: 601 PDMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660
           PDMDRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS
Sbjct: 601 PDMDRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660

Query: 661 SGKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAEN 720
           SG PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAEN
Sbjct: 661 SGNPEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAEN 720

Query: 721 GTLVRVASLEKLYKIFERC 736
           G+ +RVASLEKLYKIFERC
Sbjct: 721 GSFIRVASLEKLYKIFERC 739

BLAST of MC11g1265 vs. ExPASy TrEMBL
Match: A0A6J1E2L8 (DNA mismatch repair protein MLH1 OS=Momordica charantia OX=3673 GN=LOC111025452 PE=3 SV=1)

HSP 1 Score: 1439 bits (3725), Expect = 0.0
Identity = 736/736 (100.00%), Postives = 736/736 (100.00%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR
Sbjct: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY
Sbjct: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE
Sbjct: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV
Sbjct: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG
Sbjct: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE
Sbjct: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM
Sbjct: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK
Sbjct: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660

Query: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720
           PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL
Sbjct: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720

Query: 721 VRVASLEKLYKIFERC 736
           VRVASLEKLYKIFERC
Sbjct: 721 VRVASLEKLYKIFERC 736

BLAST of MC11g1265 vs. ExPASy TrEMBL
Match: A0A6J1ICV0 (DNA mismatch repair protein MLH1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471389 PE=3 SV=1)

HSP 1 Score: 1299 bits (3362), Expect = 0.0
Identity = 658/736 (89.40%), Postives = 697/736 (94.70%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PMET GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMETAGEEEVPREEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSGSKSQK+PV KMVRTDSTDPAGRLHAYV
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGSKSQKIPVQKMVRTDSTDPAGRLHAYV 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+Y+G
Sbjct: 421 QMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCVYIG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHFNAIQLSNPAPL ELI LAL+E
Sbjct: 481 MADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLALRE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYTPDM
Sbjct: 541 EDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSG 
Sbjct: 601 DRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGN 660

Query: 661 PEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGTL 720
           PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAENG+ 
Sbjct: 661 PEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAENGSF 720

Query: 721 VRVASLEKLYKIFERC 736
           +RVASLEKLYKIFERC
Sbjct: 721 IRVASLEKLYKIFERC 736

BLAST of MC11g1265 vs. ExPASy TrEMBL
Match: A0A6J1IBD7 (DNA mismatch repair protein MLH1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471389 PE=3 SV=1)

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 658/739 (89.04%), Postives = 697/739 (94.32%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PMET GEEE PR+EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMETAGEEEVPREEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY+AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYIAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSG---SKSQKVPVHKMVRTDSTDPAGRLH 420
           NDTRTFQEQDVESSAA QMV+S+DY QNSSQSG   SKSQK+PV KMVRTDSTDPAGRLH
Sbjct: 361 NDTRTFQEQDVESSAASQMVISSDYTQNSSQSGTEGSKSQKIPVQKMVRTDSTDPAGRLH 420

Query: 421 AYVQMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCI 480
           AYVQMKPPGLPESSL  VRSFVR RRNPKE ANLTS+QDLV EID+NCHAGLLN VRHC+
Sbjct: 421 AYVQMKPPGLPESSLNTVRSFVRMRRNPKEAANLTSVQDLVAEIDQNCHAGLLNTVRHCV 480

Query: 481 YVGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILA 540
           Y+GMADDVFALLQHDTHLYLA+VV+LSKELMYQQVL RFAHFNAIQLSNPAPL ELI LA
Sbjct: 481 YIGMADDVFALLQHDTHLYLADVVSLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLA 540

Query: 541 LKEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYT 600
           L+EEDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYT
Sbjct: 541 LREEDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDINGNLARLPVVLDQYT 600

Query: 601 PDMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660
           PDMDRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS
Sbjct: 601 PDMDRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660

Query: 661 SGKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAEN 720
           SG PEDE+IGD  + MENEI+HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAEN
Sbjct: 661 SGNPEDENIGDDTMTMENEIDHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAEN 720

Query: 721 GTLVRVASLEKLYKIFERC 736
           G+ +RVASLEKLYKIFERC
Sbjct: 721 GSFIRVASLEKLYKIFERC 739

BLAST of MC11g1265 vs. ExPASy TrEMBL
Match: A0A6J1EVP8 (DNA mismatch repair protein MLH1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111438418 PE=3 SV=1)

HSP 1 Score: 1288 bits (3332), Expect = 0.0
Identity = 655/737 (88.87%), Postives = 692/737 (93.89%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PM+T GEEE P +EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMDTGGEEEVPPQEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYTAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 420
           NDTRTFQEQDVESSAA QMV+ +DY QNSSQSGSKSQKVPV KMVRTDSTDPAGRLHAYV
Sbjct: 361 NDTRTFQEQDVESSAASQMVIRSDYTQNSSQSGSKSQKVPVQKMVRTDSTDPAGRLHAYV 420

Query: 421 QMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIYVG 480
           QM PPGLPESSL  VRSFVR RRNP+E ANLTS+QDLV EID+NCHAGLLN VRHC+Y+G
Sbjct: 421 QMNPPGLPESSLNTVRSFVRMRRNPREAANLTSVQDLVAEIDQNCHAGLLNTVRHCVYIG 480

Query: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILALKE 540
           MADDVFALLQHDTHLYLANVVNLSKELMYQQVL RFAHFNAIQLSNPAPL ELI LAL+E
Sbjct: 481 MADDVFALLQHDTHLYLANVVNLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLALRE 540

Query: 541 EDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTPDM 600
           EDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYTPDM
Sbjct: 541 EDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDVNGNLARLPVVLDQYTPDM 600

Query: 601 DRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGK 660
           DRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSG 
Sbjct: 601 DRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSSGN 660

Query: 661 PEDEDIG-DGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENGT 720
           PEDE+IG D  + MENEI HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAENG+
Sbjct: 661 PEDENIGADDTMTMENEINHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAENGS 720

Query: 721 LVRVASLEKLYKIFERC 736
            +RVASLE+LYKIFERC
Sbjct: 721 FIRVASLERLYKIFERC 737

BLAST of MC11g1265 vs. ExPASy TrEMBL
Match: A0A6J1EVF2 (DNA mismatch repair protein MLH1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111438418 PE=3 SV=1)

HSP 1 Score: 1287 bits (3330), Expect = 0.0
Identity = 655/739 (88.63%), Postives = 692/739 (93.64%), Query Frame = 0

Query: 1   METHADDEIVPMETDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 60
           ME HADDEI+PM+T GEEE P +EPPKILRL  SVVNRIAAGEVIQRPVSAIKELVENSL
Sbjct: 1   MEPHADDEIIPMDTGGEEEVPPQEPPKILRLDNSVVNRIAAGEVIQRPVSAIKELVENSL 60

Query: 61  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 120
           DAQSTS+NVVVKDGGLKLIQVSDDGHGIR+EDLPILCERHTTSKLSKFEDLQSI+SMGFR
Sbjct: 61  DAQSTSVNVVVKDGGLKLIQVSDDGHGIRYEDLPILCERHTTSKLSKFEDLQSIRSMGFR 120

Query: 121 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 180
           GEALASMTYVGHVTVTTITKGQL+GYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNM+
Sbjct: 121 GEALASMTYVGHVTVTTITKGQLHGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMS 180

Query: 181 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 240
           ARRKTLQN SDDYTKIVDLLSRFAIHH N SFSCRKHGAARADVHSV S+SRLDAIRTVY
Sbjct: 181 ARRKTLQNVSDDYTKIVDLLSRFAIHHINISFSCRKHGAARADVHSVGSTSRLDAIRTVY 240

Query: 241 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 300
           GASVARNLMKIEVS ND+ CSDFKMDGLISNSNY AKKITMVLFIN RMV+CS+LKRA+E
Sbjct: 241 GASVARNLMKIEVSENDKACSDFKMDGLISNSNYTAKKITMVLFINERMVDCSALKRAIE 300

Query: 301 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 360
           IVYAATLPKASKPF+YMSIILPPEHVDVNVHPTKKEV+LLNQEVIIERIQSAVESKLRSS
Sbjct: 301 IVYAATLPKASKPFIYMSIILPPEHVDVNVHPTKKEVSLLNQEVIIERIQSAVESKLRSS 360

Query: 361 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSG---SKSQKVPVHKMVRTDSTDPAGRLH 420
           NDTRTFQEQDVESSAA QMV+ +DY QNSSQSG   SKSQKVPV KMVRTDSTDPAGRLH
Sbjct: 361 NDTRTFQEQDVESSAASQMVIRSDYTQNSSQSGTAGSKSQKVPVQKMVRTDSTDPAGRLH 420

Query: 421 AYVQMKPPGLPESSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCI 480
           AYVQM PPGLPESSL  VRSFVR RRNP+E ANLTS+QDLV EID+NCHAGLLN VRHC+
Sbjct: 421 AYVQMNPPGLPESSLNTVRSFVRMRRNPREAANLTSVQDLVAEIDQNCHAGLLNTVRHCV 480

Query: 481 YVGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILA 540
           Y+GMADDVFALLQHDTHLYLANVVNLSKELMYQQVL RFAHFNAIQLSNPAPL ELI LA
Sbjct: 481 YIGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLCRFAHFNAIQLSNPAPLYELISLA 540

Query: 541 LKEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYT 600
           L+EEDVN   NEND F +KVAE  +KLLK KAEMLEEFFCIHID NGNLARLP+VLDQYT
Sbjct: 541 LREEDVNSESNENDDFNKKVAETSTKLLKLKAEMLEEFFCIHIDVNGNLARLPVVLDQYT 600

Query: 601 PDMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660
           PDMDRVPEF++SLANDIDWEDEKNC+QS+SAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS
Sbjct: 601 PDMDRVPEFVLSLANDIDWEDEKNCIQSISAAIGNFYAMHPPLLPNPSGDGLQFYKRIKS 660

Query: 661 SGKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAEN 720
           SG PEDE+IGD  + MENEI HGLLSEAET+WAQREWSIQHVL PSMKLFFKPP+SLAEN
Sbjct: 661 SGNPEDENIGDDTMTMENEINHGLLSEAETIWAQREWSIQHVLIPSMKLFFKPPHSLAEN 720

Query: 721 GTLVRVASLEKLYKIFERC 736
           G+ +RVASLE+LYKIFERC
Sbjct: 721 GSFIRVASLERLYKIFERC 739

BLAST of MC11g1265 vs. TAIR 10
Match: AT4G09140.1 (MUTL-homologue 1 )

HSP 1 Score: 1050.0 bits (2714), Expect = 8.6e-307
Identity = 538/738 (72.90%), Postives = 621/738 (84.15%), Query Frame = 0

Query: 6   DDEIVPMETDGEEEFPR-----KEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSL 65
           DD  +  E + EEE P      +EPPKI RL ESVVNRIAAGEVIQRPVSA+KELVENSL
Sbjct: 3   DDSSLTAEME-EEESPATTIVPREPPKIQRLEESVVNRIAAGEVIQRPVSAVKELVENSL 62

Query: 66  DAQSTSINVVVKDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFR 125
           DA S+SI+VVVKDGGLKLIQVSDDGHGIR EDLPILCERHTTSKL+KFEDL S+ SMGFR
Sbjct: 63  DADSSSISVVVKDGGLKLIQVSDDGHGIRREDLPILCERHTTSKLTKFEDLFSLSSMGFR 122

Query: 126 GEALASMTYVGHVTVTTITKGQLYGYRVSYRDGVMEHEPKPCAAVKGTQITVENLFYNMT 185
           GEALASMTYV HVTVTTITKGQ++GYRVSYRDGVMEHEPK CAAVKGTQI VENLFYNM 
Sbjct: 123 GEALASMTYVAHVTVTTITKGQIHGYRVSYRDGVMEHEPKACAAVKGTQIMVENLFYNMI 182

Query: 186 ARRKTLQNSSDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVVSSSRLDAIRTVY 245
           ARRKTLQNS+DDY KIVDLLSR AIH+ N SFSCRKHGA +ADVHSVVS SRLD+IR+VY
Sbjct: 183 ARRKTLQNSADDYGKIVDLLSRMAIHYNNVSFSCRKHGAVKADVHSVVSPSRLDSIRSVY 242

Query: 246 GASVARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKKITMVLFINGRMVECSSLKRAVE 305
           G SVA+NLMK+EVS  D     F M+G ISNSNYVAKK  +VLFIN R+VECS+LKRA+E
Sbjct: 243 GVSVAKNLMKVEVSSCDSSGCTFDMEGFISNSNYVAKKTILVLFINDRLVECSALKRAIE 302

Query: 306 IVYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSS 365
           IVYAATLPKASKPFVYMSI LP EHVD+N+HPTKKEV+LLNQE+IIE IQS VE KLR++
Sbjct: 303 IVYAATLPKASKPFVYMSINLPREHVDINIHPTKKEVSLLNQEIIIEMIQSEVEVKLRNA 362

Query: 366 NDTRTFQEQDVESSAAYQMVMSNDYNQNSSQSGSKSQKVPVHKMVRTDSTDPAGRLHAYV 425
           NDTRTFQEQ VE   +      +D   +   SG K+QKVPV+KMVRTDS+DPAGRLHA++
Sbjct: 363 NDTRTFQEQKVEYIQSTLTSQKSDSPVSQKPSGQKTQKVPVNKMVRTDSSDPAGRLHAFL 422

Query: 426 QMKPPGLPE--SSLTAVRSFVRRRRNPKEVANLTSIQDLVTEIDKNCHAGLLNIVRHCIY 485
           Q KP  LP+  SSL+ VRS VR+RRNPKE A+L+S+Q+L+  +D  CH G+L  VR+C Y
Sbjct: 423 QPKPQSLPDKVSSLSVVRSSVRQRRNPKETADLSSVQELIAGVDSCCHPGMLETVRNCTY 482

Query: 486 VGMADDVFALLQHDTHLYLANVVNLSKELMYQQVLHRFAHFNAIQLSNPAPLSELIILAL 545
           VGMADDVFAL+Q++THLYLANVVNLSKELMYQQ L RFAHFNAIQLS+PAPLSELI+LAL
Sbjct: 483 VGMADDVFALVQYNTHLYLANVVNLSKELMYQQTLRRFAHFNAIQLSDPAPLSELILLAL 542

Query: 546 KEEDVNPVCNENDGFKEKVAEMGSKLLKQKAEMLEEFFCIHIDKNGNLARLPLVLDQYTP 605
           KEED++P  +  D  KE++AEM ++LLK+KAEMLEE+F +HID + NL+RLP++LDQYTP
Sbjct: 543 KEEDLDPGNDTKDDLKERIAEMNTELLKEKAEMLEEYFSVHIDSSANLSRLPVILDQYTP 602

Query: 606 DMDRVPEFIVSLANDIDWEDEKNCLQSVSAAIGNFYAMHPPLLPNPSGDGLQFYKRIKSS 665
           DMDRVPEF++ L ND++WEDEK+C Q VSAAIGNFYAMHPPLLPNPSGDG+QFY + +  
Sbjct: 603 DMDRVPEFLLCLGNDVEWEDEKSCFQGVSAAIGNFYAMHPPLLPNPSGDGIQFYSK-RGE 662

Query: 666 GKPEDEDIGDGEVVMENEIEHGLLSEAETMWAQREWSIQHVLFPSMKLFFKPPNSLAENG 725
              E  D+ +G V ME+ ++  LLS+AE  WAQREWSIQHVLFPSM+LF KPP S+A NG
Sbjct: 663 SSQEKSDL-EGNVDMEDNLDQDLLSDAENAWAQREWSIQHVLFPSMRLFLKPPASMASNG 722

Query: 726 TLVRVASLEKLYKIFERC 737
           T V+VASLEKLYKIFERC
Sbjct: 723 TFVKVASLEKLYKIFERC 737

BLAST of MC11g1265 vs. TAIR 10
Match: AT4G02460.1 (DNA mismatch repair protein, putative )

HSP 1 Score: 148.7 bits (374), Expect = 1.9e-35
Identity = 114/360 (31.67%), Postives = 180/360 (50.00%), Query Frame = 0

Query: 12  METDGEEEFPRKEPPKILRLHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVV 71
           M+ D          P I  ++ +V++RI +G+VI    SA+KELVENSLDA +TSI + +
Sbjct: 1   MQGDSSPSPTTTSSPLIRPINRNVIHRICSGQVILDLSSAVKELVENSLDAGATSIEINL 60

Query: 72  KDGGLKLIQVSDDGHGIRFEDLPILCERHTTSKLSKFEDLQSIKSMGFRGEALASMTYVG 131
           +D G    QV D+G GI   +  +L  +H TSKL  F DL ++ + GFRGEAL+S+  +G
Sbjct: 61  RDYGEDYFQVIDNGCGISPTNFKVLALKHHTSKLEDFTDLLNLTTYGFRGEALSSLCALG 120

Query: 132 HVTVTTITKGQLYGYRVSY-RDGVMEHEPKPCAAVKGTQITVENLFYNMTARRKTL-QNS 191
           ++TV T TK +     +++   G++  E K    + GT +TV  LF N+  R K   +N 
Sbjct: 121 NLTVETRTKNEPVATLLTFDHSGLLTAEKKTARQI-GTTVTVRKLFSNLPVRSKEFKRNI 180

Query: 192 SDDYTKIVDLLSRFAIHHTNTSFSCRKHGAARADVHSVV-----SSSRLDAIRTVYGASV 251
             +Y K+V LL+ +A+      F C        +  SVV       S  D I TV+G S 
Sbjct: 181 RKEYGKLVSLLNAYALIAKGVRFVC--SNTTGKNPKSVVLNTQGRGSLKDNIITVFGIST 240

Query: 252 ARNLMKIEVSGNDEVCSDFKMDGLISNSNYVAKK---ITMVLFINGRMVECSSLKRAVEI 311
             +L  + +     V  D +++G +S       +        FINGR V+   + + V  
Sbjct: 241 FTSLQPVSIC----VSEDCRVEGFLSKPGQGTGRNLADRQYFFINGRPVDMPKVSKLVNE 300

Query: 312 VYAATLPKASKPFVYMSIILPPEHVDVNVHPTKKEVTLLNQEVIIERIQSAVESKLRSSN 362
           +Y  T  +   P   +  I+P    D+NV P K++V   ++  +I  ++  +     SSN
Sbjct: 301 LYKDTSSR-KYPVTILDFIVPGGACDLNVTPDKRKVFFSDETSVIGSLREGLNEIYSSSN 352

BLAST of MC11g1265 vs. TAIR 10
Match: AT4G35520.1 (MUTL protein homolog 3 )

HSP 1 Score: 93.6 bits (231), Expect = 7.2e-19
Identity = 71/227 (31.28%), Postives = 115/227 (50.66%), Query Frame = 0

Query: 31  LHESVVNRIAAGEVIQRPVSAIKELVENSLDAQSTSINVVVKDGGLKLIQVSDDGHGIRF 90
           L E V + + +G ++      ++ELV NSLDA +T +++ V       ++V DDG G+  
Sbjct: 7   LPEGVRHSMRSGIIMFDMARVVEELVFNSLDAGATKVSIFVGVVSCS-VKVVDDGSGVSR 66

Query: 91  EDLPILCERHTTSKLSKFEDLQSI-KSMGFRGEALASMTYVGHVTVTTITKGQLYGYRVS 150
           +DL +L ER+ TSK   F ++++  ++ GFRGEALAS++ +  + V T   G+  GYR  
Sbjct: 67  DDLVLLGERYATSKFHDFTNVETASETFGFRGEALASISDISLLEVRTKAIGRPNGYRKV 126

Query: 151 YRDGVMEH-EPKPCAAVKGTQITVENLFYNMTARRKTLQNSSDDYTK-IVDLLSRFAIHH 210
            +     H          GT +TV +LFY+   RRK +Q+S     + I   + R A+ H
Sbjct: 127 MKGSKCLHLGIDDDRKDSGTTVTVRDLFYSQPVRRKYMQSSPKKVLESIKKCVFRIALVH 186

Query: 211 TNTSFSCRKHGAARADVHSVVSSSRLDAIRTVYGASVARNLMKIEVS 255
           +N SFS     +      +  SSS    +    G     +L K+ V+
Sbjct: 187 SNVSFSVLDIESDEELFQTNPSSSAFSLLMRDAGTEAVNSLCKVNVT 232