MC11g0763 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0763 vs. ExPASy Swiss-Prot
Match: Q93VQ0 (Protein VACUOLELESS1 OS=Arabidopsis thaliana OX=3702 GN=VCL1 PE=1 SV=1)

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 660/865 (76.30%), Postives = 747/865 (86.36%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+W+H+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AGI L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQN VECVFWGNGVVC+TE  Q+ CI DFK   P KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQKMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLL 300
            G +QKM VS +GK+L  FTHDGR++V+  + ++  +D  CESALPP+Q+AWCGMDSVLL
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 YWDDMLLMMGPVGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGST 360
           YWD+ L+M+GPVGDPV YFYDEP+ILIPECDGVRILSN N+EFLQRVPDST +IF+IGST
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASY 420
           SPAALLYDALDHFDRRSAKADENLRLIRSSL EAVE+C+DAAGHEFDV+RQ+ LLRAASY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR P IGIPLSIQQYKLLTP VLI+RLIN + HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRVSEYLGMSQEVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADK 540
           ALR+SEYL M++EVVIMHWAC+KITAS + PD+ LLEILLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 TGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQKR PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASK 660
           EFF MIQ RV ARDLF+ YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW++GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHGPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSIN 720
           GSPLHGPRIKLI+KA +LF++TKEH FESKAAEEHAKLLK+QHELE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

Query: 721 DTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYK 780
           DTIRTCIVLGN+RAA+KVKTEFKVS+KRWYWLK FALAT +DW ALE FSKEKRP +G++
Sbjct: 721 DTIRTCIVLGNNRAAIKVKTEFKVSDKRWYWLKAFALATIKDWAALEKFSKEKRPPMGFR 780

Query: 781 PFVEACIEADEKAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKD-GELLGRLKL 840
           PFVEACI+ADEKAEALKYIPKL+D  ER E+YARIGMAKEAAD A+QA D GELL R + 
Sbjct: 781 PFVEACIDADEKAEALKYIPKLSDLVERGEAYARIGMAKEAADCAAQANDGGELLERFRK 840

Query: 841 TFAQNTAASSIFDTLRDRLSFPGVS 845
           TF QN    +IFDTL   + F G S
Sbjct: 841 TFVQN----AIFDTL--LMPFQGAS 858

BLAST of MC11g0763 vs. ExPASy Swiss-Prot
Match: Q55C58 (Vacuolar protein sorting-associated protein 16 homolog OS=Dictyostelium discoideum OX=44689 GN=vps16 PE=3 SV=2)

HSP 1 Score: 552.0 bits (1421), Expect = 1.2e-155
Identity = 321/833 (38.54%), Postives = 494/833 (59.30%), Query Frame = 0

Query: 6   VAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 65
           +AA+W+++ N  Y K E+Y M W  +DL +     +PF GPIA++RD SK V++ +++  
Sbjct: 2   IAAQWKIIGNSTYIKKEIYSMSW-DVDLKQQVSVGSPFAGPIAVMRDSSKFVEMNSQNMK 61

Query: 66  RKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 125
             L+IF  +G  +++ +W +    ++ M W + + LV V+Q+ TV  +N+  E +   FS
Sbjct: 62  PYLKIFTASGDLISQMIW-DSSKNIVAMDWIEKERLVIVLQNATVLIFNVFCEQM-TQFS 121

Query: 126 MGKECFEQNAVECVFWGNGVVCITEANQIF---CISDFKNPNPCKLSDPGIEDLPHC--- 185
           +G    E+  +EC  W +G+V +T A+Q++    I+DF   +   +  P + + P     
Sbjct: 122 LGDIVREEEILECKIWSDGIVVLTSASQLYSVPSINDFFVESGRVIRLPPLPEEPKARPE 181

Query: 186 MAVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAF 245
            A++EPQ+++S ++E+ + +    +  ++ED V+   E     P+QKM VS  GK LA F
Sbjct: 182 WAILEPQFSLSQSIEIFMSI-NGTLYLIDEDKVESQLEAT--EPIQKMVVSPCGKKLACF 241

Query: 246 THDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWD---DMLLMMGPVGDP- 305
              G LL+L +D   T  DR    A     L WCG D V++YWD   D +L     GD  
Sbjct: 242 DTKGTLLILKTDGSTTNPDRMDTKATKSPVLKWCGSDGVMMYWDSIKDPILFYFSKGDSW 301

Query: 306 VRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDR 365
            ++  D+PV L+ E DG+RI+S+   EF  +V D T+ IF+IG+TSPA++LYDA DHF  
Sbjct: 302 AKFTLDQPVSLVTEIDGLRIISDTTSEFFHKVSDVTIDIFKIGTTSPASILYDATDHFIS 361

Query: 366 RSAKADENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQE 425
           +S +ADE++R I   L +AV  C+ AAG EF+   Q  LL+AAS+G+ F  N+N  +   
Sbjct: 362 KSPQADESIRSINDQLEDAVNDCILAAGFEFNGGEQSKLLKAASFGKCFLENYNPSQFVT 421

Query: 426 MCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVV 485
           MCR LRVLNAVR  +IGIPLSI+QY  +    LI RLI+  +HLLA R+ +YL +  +VV
Sbjct: 422 MCRSLRVLNAVRHHEIGIPLSIKQYYHIGIEELIDRLISRRKHLLAWRICDYLKIKSDVV 481

Query: 486 IMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEP 545
           + HWAC+K+    +IPD  L +I++ KL+   GIS+A +A  A   GR KLA  L+++EP
Sbjct: 482 LNHWACTKVRT--DIPDQELGKIIIKKLESVPGISFANIASAAYLAGRSKLATKLLEYEP 541

Query: 546 RSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDL 605
           ++++QVP L+ +GE   AL KA ESGDTDLVYLVL  + +     +F  +  ++V A DL
Sbjct: 542 KAAEQVPPLIKMGESGLALNKAIESGDTDLVYLVLLAMQRSLPLADFLELTFSKVVALDL 601

Query: 606 FITYARCYKHEF--LKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLID 665
            I+  +  K++F  L++ +    Q  E+  +  +E+       ++S  S L   RIK  +
Sbjct: 602 LISMCK-QKNDFPLLREIYHIKDQSKEMGNIYLQEA-------LSSHPSQL-DQRIKAYN 661

Query: 666 KA--HSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGN 725
           K+  H   ++ K+    SK  ++  KL  +Q ELE + +   FV  SINDTI   I +  
Sbjct: 662 KSIEHYHHSKDKDDQATSKFIDDQIKLEMLQKELETNLQDESFVGISINDTIYKLITMNQ 721

Query: 726 HRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADE 785
            + A  +++EFKV +KR++W+K+ AL+   DW  L  FSKEK+  IGY+PFVE C++   
Sbjct: 722 PKKAQSIRSEFKVPDKRFWWIKIKALSIMNDWEELMKFSKEKKSPIGYEPFVEVCLDQKN 781

Query: 786 KAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQN 825
           + EALKYIPK+ D   + + Y +IG  +EAAD A + K+ +LL  +      N
Sbjct: 782 QIEALKYIPKITDILPKIQFYIQIGYFREAADIAFKEKNFDLLNLISRKCTNN 817

BLAST of MC11g0763 vs. ExPASy Swiss-Prot
Match: Q9H269 (Vacuolar protein sorting-associated protein 16 homolog OS=Homo sapiens OX=9606 GN=VPS16 PE=1 SV=2)

HSP 1 Score: 435.3 bits (1118), Expect = 1.6e-120
Identity = 281/828 (33.94%), Postives = 450/828 (54.35%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRW-RHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +  A S  
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWR--KEKAASVR 65

Query: 68  RKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +G+ LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLDIYSASGMPLASLLWKS--GPVVSLGWSAEEELLCVQEDGAVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNAVEC-VF---WGNGVVCITEANQIFCISDFKNPNPCKLSD-PGIEDLPHCM 187
           MG E  +   ++  +F   +G+GV  +T A++    ++  +    ++ + PG++  P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCW 185

Query: 188 AVIEPQYTMSGNVEVLLGVG-------EACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDG 247
            V+           +LL VG        A   AV   G   L  GV      +MAVS   
Sbjct: 186 TVL----CQDRVAHILLAVGPDLYLLDHAACSAVTPPG---LAPGV--SSFLQMAVSFTY 245

Query: 248 KWLAAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMD-----SVLLYWDDMLLM 307
           + LA FT  G + + T+ L++ + +  C    PP+Q+ WC        +V++ W+  L++
Sbjct: 246 RHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMV 305

Query: 308 MGPVGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYD 367
           +G   + +++  DE   L+PE DGVRI S +  EFL  VP ++  IF+I S +P ALL +
Sbjct: 306 VGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLE 365

Query: 368 ALDHFDRRSAKADENLRLIR--SSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCS 427
           A   +++ S KADE LR I+    L +AV+ C++AAGHE     Q++LLRAAS+G+ F  
Sbjct: 366 AQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLD 425

Query: 428 NFNRERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSE 487
            F  +    MC+ LRVLNAVR   IGIPL+  QYK LT  VL+ RL+    + LA+++ E
Sbjct: 426 RFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICE 485

Query: 488 YLGMSQ----EVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTG 547
           YL + +      ++ HWAC K+    ++ D  +   +  KL    G+SY+ +A  A   G
Sbjct: 486 YLRLPEVQGVSRILAHWACYKVQQK-DVSDEDVARAINQKLGDTPGVSYSDIAARAYGCG 545

Query: 548 RRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEF 607
           R +LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +F
Sbjct: 546 RTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDF 605

Query: 608 FGMIQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGS 667
           F  ++ +  A  L+  + +  + E LKD +       E+     + S+   +        
Sbjct: 606 FMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEER------- 665

Query: 668 PLHGPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDT 727
            + G R+  +  A   F + K   F +KA E+  +LL++Q  LE       F+D S++DT
Sbjct: 666 -IEG-RVAALQTAADAFYKAKNE-FAAKATEDQMRLLRLQRRLE-DELGGQFLDLSLHDT 725

Query: 728 IRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPF 787
           + T I+ G+++ A ++  +F++ +KR +WLK+ ALA   DW  LE FSK K+  IGY PF
Sbjct: 726 VTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPF 785

Query: 788 VEACIEADEKAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKD 811
           VE C++   K EA KY  ++  P ++ ++   +G   +AAD A + ++
Sbjct: 786 VEICMKQHNKYEAKKYASRVG-PEQKVKALLLVGDVAQAADVAIEHRN 806

BLAST of MC11g0763 vs. ExPASy Swiss-Prot
Match: Q5E9L7 (Vacuolar protein sorting-associated protein 16 homolog OS=Bos taurus OX=9913 GN=VPS16 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.8e-119
Identity = 278/828 (33.57%), Postives = 447/828 (53.99%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRW-RHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +    SA 
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWR--KEKPASAR 65

Query: 68  RKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +G+ LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLEIYSASGVPLASLLWKS--GPVVSLGWSAEEELLCVQEDGVVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNAVEC-VF---WGNGVVCITEANQIFCISDFKNPNPCKLSD-PGIEDLPHCM 187
           MG E  +   ++  +F   +G+GV  +T A++    ++  +    ++ + PG+   P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLXSAPSCW 185

Query: 188 AVIEPQYTMSGNVEVLLGVG-------EACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDG 247
             +           +LL VG        A   AV   G   L  GV      +MAVS   
Sbjct: 186 TTV----CQDRVAHILLAVGPDLYLLDHAACSAVTPPG---LAPGV--SSFLQMAVSFTY 245

Query: 248 KWLAAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMD-----SVLLYWDDMLLM 307
           + LA FT  G + + T+ L++ + +  C    PP+Q+ WC        +V++ W+  L++
Sbjct: 246 RHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMV 305

Query: 308 MGPVGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYD 367
           +G   + +++  DE   L+PE DGVR+ S +  EFL  VP ++  IF+I S +P ALL +
Sbjct: 306 VGDAPESIQFVLDEDSYLVPELDGVRVFSRSTHEFLHEVPVASEEIFKIASMAPGALLLE 365

Query: 368 ALDHFDRRSAKADENLRLIR--SSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCS 427
           A   +++ S KADE LR I+    L +AV+ C++AAGHE     Q++LLRAAS+G+ F  
Sbjct: 366 AQKEYEKESQKADEYLREIQELGQLPQAVQQCIEAAGHEHWPDMQKSLLRAASFGKCFLD 425

Query: 428 NFNRERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSE 487
            F  +    MC+ LRVLNA+R   IGIPL+  QYK LT  VL+ RL+    + LA+++ E
Sbjct: 426 RFPPDSFVRMCQDLRVLNAIRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICE 485

Query: 488 YLGMSQ----EVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTG 547
           YL + +      ++ HWAC K+    ++ D  +   +  KL    G+SY+ +A  A   G
Sbjct: 486 YLRLPEVQGVSRILAHWACYKVQQK-DVSDEDVARAINQKLGDTPGVSYSDIAARAYGCG 545

Query: 548 RRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEF 607
           R +LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +F
Sbjct: 546 RTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDF 605

Query: 608 FGMIQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGS 667
           F  ++ +  A  L+  + +  + E LKD +       E+     + S+   +        
Sbjct: 606 FMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEER------- 665

Query: 668 PLHGPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDT 727
            + G R+  +  A   F + K   F +KA E+  +LL++Q  LE       F+D S++DT
Sbjct: 666 -IEG-RVAALQTAADAFYKAKNE-FAAKATEDQMRLLRLQRRLE-DELGGQFLDLSLHDT 725

Query: 728 IRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPF 787
           + T I+ G ++ A ++  +F++ +KR +WLK+ ALA   DW  LE FSK K+  IGY PF
Sbjct: 726 VTTLILSGQNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPF 785

Query: 788 VEACIEADEKAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKD 811
           VE C++   K EA KY  ++  P ++ ++   +G   +AAD A + ++
Sbjct: 786 VEICMKQHNKYEAKKYASRVG-PEQKVKALLLVGDVAQAADVAIEHRN 806

BLAST of MC11g0763 vs. ExPASy Swiss-Prot
Match: Q920Q4 (Vacuolar protein sorting-associated protein 16 homolog OS=Mus musculus OX=10090 GN=Vps16 PE=1 SV=3)

HSP 1 Score: 426.4 bits (1095), Expect = 7.5e-118
Identity = 276/825 (33.45%), Postives = 451/825 (54.67%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRW-RHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +  A S  
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWR--KEKAASVR 65

Query: 68  RKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +G+ LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLEIYSASGLPLASLLWKS--GPVVALGWSAEEELLCVQEDGAVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNAVEC-VF---WGNGVVCITEANQIFCISDFKNPNPCKLSD-PGIEDLPHCM 187
           MG E  +   ++  +F   +G+GV  +T A +    ++  +    ++ + PG++  P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCW 185

Query: 188 AVI----EPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWL 247
             +     P   ++   ++ L +  A   AV   G   L  GV      +MAVS   ++L
Sbjct: 186 TTLCHDRVPHILLAVGPDLYL-LDHATCSAVTPAG---LAPGV--SSFLQMAVSFTYRYL 245

Query: 248 AAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMD-----SVLLYWDDMLLMMGP 307
           A FT  G + + T+ L++ + +  C    PP+Q+ WC        +V++ W+  L+++G 
Sbjct: 246 ALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGN 305

Query: 308 VGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALD 367
             + +++  DE   L+PE DGVRI S +  EFL  VP ++  IF+I S +P ALL +A  
Sbjct: 306 APESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQK 365

Query: 368 HFDRRSAKADENLRLIR--SSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFN 427
            +++ S KADE LR I+    L +AV+ C++AAGHE     Q++LLRAAS+G+ F   F 
Sbjct: 366 EYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFP 425

Query: 428 RERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLG 487
            +    MC+ LRVLNA+R   IGIPL+  QYK LT  VL+ RL+    + LA+++ EYL 
Sbjct: 426 PDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLR 485

Query: 488 MSQ----EVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRK 547
           + +      ++ HWAC K  A  ++ D  +   +  KL    G+SY+ +A  A   GR +
Sbjct: 486 LPEVQGVSRILAHWACYKARA-WDMRDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTE 545

Query: 548 LAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGM 607
           LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +FF  
Sbjct: 546 LAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMT 605

Query: 608 IQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLH 667
           ++ +  A  L+  + +  + + LKD +       E+     + S+   +         + 
Sbjct: 606 LRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEER--------IE 665

Query: 668 GPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRT 727
           G R+  +  A   F + K   F +KA E+  +LL++Q  LE       F+D S++DT+ T
Sbjct: 666 G-RVAALQTAADAFYKAKNE-FAAKATEDQMRLLRIQRRLE-DELGGRFLDLSLHDTVTT 725

Query: 728 CIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEA 787
            I+ G+++ A ++  +F++ +KR +WLK+ ALA   DW  LE FSK K+  IGY PFVE 
Sbjct: 726 LILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEI 785

Query: 788 CIEADEKAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKD 811
           C++   K EA KY  ++  P ++ ++   +G   +AA+ A + ++
Sbjct: 786 CMKQHNKHEAKKYASRVG-PEQKVKALLLVGDVAQAAEVAIEHRN 806

BLAST of MC11g0763 vs. NCBI nr
Match: XP_022135034.1 (protein VACUOLELESS1 isoform X1 [Momordica charantia])

HSP 1 Score: 1690 bits (4376), Expect = 0.0
Identity = 844/844 (100.00%), Postives = 844/844 (100.00%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. NCBI nr
Match: XP_022135042.1 (protein VACUOLELESS1 isoform X2 [Momordica charantia])

HSP 1 Score: 1681 bits (4353), Expect = 0.0
Identity = 842/844 (99.76%), Postives = 842/844 (99.76%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK  PLEFFGMIQARVQARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK--PLEFFGMIQARVQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 842

BLAST of MC11g0763 vs. NCBI nr
Match: XP_008465445.1 (PREDICTED: protein VACUOLELESS1 [Cucumis melo] >XP_008465446.1 PREDICTED: protein VACUOLELESS1 [Cucumis melo])

HSP 1 Score: 1637 bits (4240), Expect = 0.0
Identity = 812/844 (96.21%), Postives = 829/844 (98.22%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGP+AIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPVAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNP  CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPP+QLAWCGMDSVLLYWDDMLLMMGP GDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV+LIPECDGVRILSN +MEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSP+IGIPLSIQQ+KLLTP VLIARLIN HQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQAR QARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESWELGKNPMASKGSPLH PR KLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHIPRTKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH FESKAAEEHAKLLK+QHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHTFESKAAEEHAKLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. NCBI nr
Match: XP_038898258.1 (protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898259.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898260.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898261.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1632 bits (4225), Expect = 0.0
Identity = 807/844 (95.62%), Postives = 827/844 (97.99%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAET+WRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETIWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNP  CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPP+QLAWCGMDSVLLYWDDMLLMMGP GDPVRY Y
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV+LIPECDGVRILSN +MEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSP+IGIPLS+QQ+KLLTPSVLIARLIN HQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSVQQFKLLTPSVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS N+PDATLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASSNVPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGD+DLVYLVLFHIWQKRQPLEFFGMIQAR+QARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDSDLVYLVLFHIWQKRQPLEFFGMIQARIQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQL EVAFLLWKESWELGKNPMASKGSPLH PR KLI+KAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLDEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHA+LLK+QHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHMFESKAAEEHARLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEACIEADEK E LKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKVETLKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. NCBI nr
Match: XP_004144632.1 (protein VACUOLELESS1 [Cucumis sativus] >XP_031739894.1 protein VACUOLELESS1 [Cucumis sativus] >KGN54934.1 hypothetical protein Csa_012058 [Cucumis sativus])

HSP 1 Score: 1630 bits (4221), Expect = 0.0
Identity = 807/844 (95.62%), Postives = 827/844 (97.99%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNPN CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPNACKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEG+LDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGILDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQK ILDRECESALPP+QLAWCGMDSVLLYWDDMLLMMGP GDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKIILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV LIPECDGVRILSN +MEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVFLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVR+P+IGIPLSIQQ+KLLTP VLIARLIN HQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS NI DATLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASANIADATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQAR QARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESWELGKNPMASKGSPLH PR KLI+KAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHAKLLK+QH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHIFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. ExPASy TrEMBL
Match: A0A6J1C0C3 (Protein VACUOLELESS1 OS=Momordica charantia OX=3673 GN=LOC111007126 PE=3 SV=1)

HSP 1 Score: 1690 bits (4376), Expect = 0.0
Identity = 844/844 (100.00%), Postives = 844/844 (100.00%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. ExPASy TrEMBL
Match: A0A6J1C1I9 (Protein VACUOLELESS1 OS=Momordica charantia OX=3673 GN=LOC111007126 PE=3 SV=1)

HSP 1 Score: 1681 bits (4353), Expect = 0.0
Identity = 842/844 (99.76%), Postives = 842/844 (99.76%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK  PLEFFGMIQARVQARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK--PLEFFGMIQARVQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 842

BLAST of MC11g0763 vs. ExPASy TrEMBL
Match: A0A1S3CQD9 (Protein VACUOLELESS1 OS=Cucumis melo OX=3656 GN=LOC103503053 PE=3 SV=1)

HSP 1 Score: 1637 bits (4240), Expect = 0.0
Identity = 812/844 (96.21%), Postives = 829/844 (98.22%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGP+AIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPVAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNP  CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPP+QLAWCGMDSVLLYWDDMLLMMGP GDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV+LIPECDGVRILSN +MEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVRSP+IGIPLSIQQ+KLLTP VLIARLIN HQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDATLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQAR QARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESWELGKNPMASKGSPLH PR KLIDKAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHIPRTKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH FESKAAEEHAKLLK+QHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHTFESKAAEEHAKLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. ExPASy TrEMBL
Match: A0A0A0L1K9 (Protein VACUOLELESS1 OS=Cucumis sativus OX=3659 GN=Csa_4G608100 PE=3 SV=1)

HSP 1 Score: 1630 bits (4221), Expect = 0.0
Identity = 807/844 (95.62%), Postives = 827/844 (97.99%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGIQLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNPN CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPNACKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEG+LDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGILDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQK ILDRECESALPP+QLAWCGMDSVLLYWDDMLLMMGP GDPVRYFY
Sbjct: 241 HDGRLLVLTSDLQKIILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV LIPECDGVRILSN +MEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVFLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRAASYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVR+P+IGIPLSIQQ+KLLTP VLIARLIN HQHLLALRVSEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS NI DATLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASANIADATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQAR QARDLFITYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESWELGKNPMASKGSPLH PR KLI+KAHSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHAKLLK+QH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHIFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. ExPASy TrEMBL
Match: A0A6J1FI17 (Protein VACUOLELESS1 OS=Cucurbita moschata OX=3662 GN=LOC111445955 PE=3 SV=1)

HSP 1 Score: 1627 bits (4213), Expect = 0.0
Identity = 805/844 (95.38%), Postives = 826/844 (97.87%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNP  CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEGVLDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300
           HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGP GDPVRY Y
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360
           DEPV+LIPECDGVRILSN + EFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA
Sbjct: 301 DEPVVLIPECDGVRILSNTSTEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480
           RVLNAVR+P+IGIPLSIQQ+KLLT  VLIARLIN HQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPD TLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR+QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESW LGKNPMASKGSPLH PRIKLI+K  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLK+QH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 780

Query: 781 KLADPRERAESYARIGMAKEAADAASQAKDGELLGRLKLTFAQNTAASSIFDTLRDRLSF 840
           KLADPRERAE+YARIGMAKEAADAASQAKDGELLGRLKLTFAQN+AASSIFDTLRDRLSF
Sbjct: 781 KLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSF 840

Query: 841 PGVS 844
           PGVS
Sbjct: 841 PGVS 844

BLAST of MC11g0763 vs. TAIR 10
Match: AT2G38020.1 (vacuoleless1 (VCL1) )

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 660/865 (76.30%), Postives = 747/865 (86.36%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+W+H+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AGI L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQN VECVFWGNGVVC+TE  Q+ CI DFK   P KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQKMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLL 300
            G +QKM VS +GK+L  FTHDGR++V+  + ++  +D  CESALPP+Q+AWCGMDSVLL
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 YWDDMLLMMGPVGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGST 360
           YWD+ L+M+GPVGDPV YFYDEP+ILIPECDGVRILSN N+EFLQRVPDST +IF+IGST
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASY 420
           SPAALLYDALDHFDRRSAKADENLRLIRSSL EAVE+C+DAAGHEFDV+RQ+ LLRAASY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR P IGIPLSIQQYKLLTP VLI+RLIN + HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRVSEYLGMSQEVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADK 540
           ALR+SEYL M++EVVIMHWAC+KITAS + PD+ LLEILLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 TGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQKR PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASK 660
           EFF MIQ RV ARDLF+ YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW++GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHGPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSIN 720
           GSPLHGPRIKLI+KA +LF++TKEH FESKAAEEHAKLLK+QHELE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

Query: 721 DTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYK 780
           DTIRTCIVLGN+RAA+KVKTEFKVS+KRWYWLK FALAT +DW ALE FSKEKRP +G++
Sbjct: 721 DTIRTCIVLGNNRAAIKVKTEFKVSDKRWYWLKAFALATIKDWAALEKFSKEKRPPMGFR 780

Query: 781 PFVEACIEADEKAEALKYIPKLADPRERAESYARIGMAKEAADAASQAKD-GELLGRLKL 840
           PFVEACI+ADEKAEALKYIPKL+D  ER E+YARIGMAKEAAD A+QA D GELL R + 
Sbjct: 781 PFVEACIDADEKAEALKYIPKLSDLVERGEAYARIGMAKEAADCAAQANDGGELLERFRK 840

Query: 841 TFAQNTAASSIFDTLRDRLSFPGVS 845
           TF QN    +IFDTL   + F G S
Sbjct: 841 TFVQN----AIFDTL--LMPFQGAS 858

BLAST of MC11g0763 vs. TAIR 10
Match: AT2G38020.2 (vacuoleless1 (VCL1) )

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 627/810 (77.41%), Postives = 709/810 (87.53%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+W+H+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AGI L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQN VECVFWGNGVVC+TE  Q+ CI DFK   P KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQKMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLL 300
            G +QKM VS +GK+L  FTHDGR++V+  + ++  +D  CESALPP+Q+AWCGMDSVLL
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 YWDDMLLMMGPVGDPVRYFYDEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGST 360
           YWD+ L+M+GPVGDPV YFYDEP+ILIPECDGVRILSN N+EFLQRVPDST +IF+IGST
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASY 420
           SPAALLYDALDHFDRRSAKADENLRLIRSSL EAVE+C+DAAGHEFDV+RQ+ LLRAASY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR P IGIPLSIQQYKLLTP VLI+RLIN + HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRVSEYLGMSQEVVIMHWACSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADK 540
           ALR+SEYL M++EVVIMHWAC+KITAS + PD+ LLEILLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 TGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQKR PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARVQARDLFITYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASK 660
           EFF MIQ RV ARDLF+ YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW++GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHGPRIKLIDKAHSLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSIN 720
           GSPLHGPRIKLI+KA +LF++TKEH FESKAAEEHAKLLK+QHELE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

Query: 721 DTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYK 780
           DTIRTCIVLGN+RAA+KVKTEFKVS+KRWYWLK FALAT +DW ALE FSKEKRP +G++
Sbjct: 721 DTIRTCIVLGNNRAAIKVKTEFKVSDKRWYWLKAFALATIKDWAALEKFSKEKRPPMGFR 780

Query: 781 PFVEACIEADEKAEALKYIPKLADPRERAE 791
           PFVEACI+ADEKAEALKYIPKL+D  ER E
Sbjct: 781 PFVEACIDADEKAEALKYIPKLSDLVERGE 809