MC11g0732 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match: O65709 (Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 SV=2)

HSP 1 Score: 863.2 bits (2229), Expect = 2.3e-249
Identity = 436/747 (58.37%), Postives = 556/747 (74.43%), Query Frame = 0

Query: 54  MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 113
           MESL A+CDTF+ S+  +  GH +         A +F  SAS  GTP+RV  ++SER+ H
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVD------DCVAGYFSASASQTGTPDRVARLMSERLHH 60

Query: 114 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 173
           PKKW++R  LW+LSTW G+ +LCG  S +  FPYF+ F +L   +RE+I L+WS S+F L
Sbjct: 61  PKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSL 120

Query: 174 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 233
            RM FRTIK++S L FF+QVDEK  N++WKAI Y GP P    +    E     E  + +
Sbjct: 121 LRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSP----DHSDHEVELNEEKKKKK 180

Query: 234 EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTAS---PSMMIKCDAVVVG 293
            EE++GPL+ GIV++   R    + L   GF V+     T  +S   P M I+CDAVVVG
Sbjct: 181 PEEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVG 240

Query: 294 SGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLV 353
           SGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GLLAT + NV++
Sbjct: 241 SGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVI 300

Query: 354 LAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIE 413
           LAGSTVGGGST+NWSAS +TP HV +EW++  +L++F S+LY+EAM+VVCK+MGVQ    
Sbjct: 301 LAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFV 360

Query: 414 SEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSG 473
            EGFNN VLRKGCE+LG    NIPRN+P+DHYCG+CCLGCK G+K+GT+ETWLVDLV S 
Sbjct: 361 EEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESD 420

Query: 474 NGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTP 533
           NG ILPGC+A  +M++ + G ++K+A G+ F FG     +++ +VES+VTIVACGAL TP
Sbjct: 421 NGLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTP 480

Query: 534 PLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGT 593
            LLKRSGL+N NIG++L LHPV MAWG FP E   DK WPE+ KKSYEGGIMTAM ++  
Sbjct: 481 HLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEE---DK-WPEKKKKSYEGGIMTAMSSVVI 540

Query: 594 EADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTV 653
           E +    YG  VIQTPALHPG+FS ++PW S  +FK RM KFSRTAH+FAL RDKG+GT+
Sbjct: 541 E-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTI 600

Query: 654 RSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKF 713
            S + I Y ++  DEE+L+ G+E+ L+ILAAAGAEEIGTHH++GRS+ V+  S  E E+F
Sbjct: 601 DSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERF 660

Query: 714 VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTAL 773
           V+EESS+PL DL+  ICSAHQMGSCRMGI P++S V   GETWE+E LF+AD+SVFPTAL
Sbjct: 661 VREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTAL 720

Query: 774 GVNPMVTVQAIAYCTAQSVLDTLKRKR 798
           GVNPMVTVQ+IAYC   +V+D LK+K+
Sbjct: 721 GVNPMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.7e-159
Identity = 316/755 (41.85%), Postives = 453/755 (60.00%), Query Frame = 0

Query: 50  TAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISE 109
           +++EMESLA++C+  LP +          +  +      F+ TSAS    P  V  ++S+
Sbjct: 23  SSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNTSASHYPIPHEVAEILSK 82

Query: 110 RVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLS 169
           R       ++R+ LW+L+T  GT++LCG    S  +P+   FS +S  KRE++   W  +
Sbjct: 83  RSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFSSMSLKKRERVMQWWLKN 142

Query: 170 FFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNE 229
            F+   R+ F  +K+L L A+F+ VDE  +N +WKAI Y         E    E    + 
Sbjct: 143 RFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGY---------EVSADEKLPNSS 202

Query: 230 NGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAV 289
           NGR  E        +GIV  + +      ++L + G  V        + +  + +KCDA+
Sbjct: 203 NGRLLE--------KGIVETMHETNSTLQQSLTEKGLNVTL-----DSRNNILKVKCDAI 262

Query: 290 VVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMN 349
           VVGSG GGGV A VL+ AG+KV+VLEKG+Y A  + S LEG +M+++Y + G+LA+ +  
Sbjct: 263 VVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVDSR 322

Query: 350 VLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQS 409
           +L+LAGSTVGGGS VNWSA  +TP  V +EWS+ + L LFS++ Y  AME VC+++GV  
Sbjct: 323 ILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGVTE 382

Query: 410 EIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLV 469
               EGF N VLRKGC+ LG     +PRNS  +H+CG C  GC  G K+GT +TWLVD V
Sbjct: 383 SCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVDAV 442

Query: 470 RSGNGAILPGCEAVRIMWE-KKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGA 529
            +G   I+ GC+A R + E  ++GS RK+           S       +E+KVTI A GA
Sbjct: 443 ENG-AVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGGA 502

Query: 530 LSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMC 589
           L TPPL+  SGL+N NIGK+LHLHPV M WG+FP  T   K       K +EGGI+T++ 
Sbjct: 503 LLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKG------KIFEGGIITSVH 562

Query: 590 NIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKG 649
            + +   N      A+I+TP+L PG F+ L PW SG +FK RM  + RTAH+  + RDK 
Sbjct: 563 KVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKA 622

Query: 650 SGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYRE 709
            G V +   +SYK+   D+EN++ G+++ALRIL AAGA E+GTH + G+ +K  +VS  E
Sbjct: 623 CGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKCGEVSENE 682

Query: 710 YEKFVKEESSRPLNDLATP------ICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFI 769
             +F+  +S  P+    +P        SAHQMGSCRMG+  K+  V++ GE+WE +GLF+
Sbjct: 683 MAEFI--DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESWEAQGLFV 742

Query: 770 ADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKR 796
            D+S+ PTA+GVNPM+T+Q+ AYC +  V+D LKR
Sbjct: 743 CDASLLPTAVGVNPMITIQSTAYCISNRVVDYLKR 746

BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 9.8e-152
Identity = 316/768 (41.15%), Postives = 460/768 (59.90%), Query Frame = 0

Query: 43  YSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPER 102
           YSH   L++++M  +AA+C+   PS    +    N +SS   A +AF+  S S A  P+ 
Sbjct: 23  YSH--GLSSSQMHVIAAICEALFPSQPLDS---QNNQSSVDKALSAFYTASGSQAPLPDE 82

Query: 103 VGGMISERVRHPKKWMVRLS--LWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKRE 162
              ++ +  R   + +  +S  L +LS   GT +LCG   L   +P+   FS++   KRE
Sbjct: 83  AAELLFKFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKFSEIPLEKRE 142

Query: 163 KIALSWSL-SFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETR 222
           +I   WS    ++  R+ F   K++     FS+ D   +N  WKAI Y       +++TR
Sbjct: 143 EILKRWSREKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGY-------QVDTR 202

Query: 223 QSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPS 282
           +     L +  R  +E L   ++       +      ++L + G  V      T+    +
Sbjct: 203 E----KLTQKKRPLQEGLIETMY-------ETDSTLIQSLTEKGLEV------TEDLEQN 262

Query: 283 M-MIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 342
           M  IKCDAV+VGSG GGGV A VLAN+G+KV++LEKG Y    + S LEG +M E+Y S 
Sbjct: 263 MYKIKCDAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESG 322

Query: 343 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 402
           G+L + +  +++LAGST+GG S +NWSA  +TP  V REWS+ +++ LF+S  Y+ AM+ 
Sbjct: 323 GILPSLDGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDT 382

Query: 403 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 462
           VC+++GV      E F N +LR+GC ++G+    +  NS ADHYCG CC GC+ G KKGT
Sbjct: 383 VCRRIGVTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGT 442

Query: 463 AETWLVDLVRSGNGA-ILPGCEAVRIMWEKKD---GSERKRAKGITFEFGCQSGAKQMCL 522
             TWLVD V  GNGA IL GC+A ++ +  KD   G++RK   G+      +S   +   
Sbjct: 443 ESTWLVDAV--GNGAVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSKVTKKLQ 502

Query: 523 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 582
           +ESKVTI ACG+LSTPPL+  SGL+NPNIGK+LHLHP   AWG+FP +  N         
Sbjct: 503 IESKVTISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSG------ 562

Query: 583 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 642
            +YEGGI+T++  +  E  +       +I+ PAL PG FS L+PWVSG + K RM K++R
Sbjct: 563 NNYEGGIITSIHKVFEE--DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYAR 622

Query: 643 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 702
           TA++FAL RD GSG V++   ISYK+D  D E+LQ G+ KALRIL AAGA E+GT+ + G
Sbjct: 623 TANLFALVRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDG 682

Query: 703 RSIKVKKVSYREYEKF------VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQ 762
           + IK + +   + E+F      V   SSR  N++ T   SAHQM SCRM    ++  V++
Sbjct: 683 QRIKCRGIKESDLEEFLDSVRVVGGPSSR--NEVWTVFTSAHQMTSCRMSATEEEGAVDE 742

Query: 763 RGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
            GE+WE +GL++ D SV P+A+GVNPM+T+Q+ AYC A ++ ++LK++
Sbjct: 743 NGESWEAKGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKKQ 749

BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.1e-147
Identity = 313/763 (41.02%), Postives = 449/763 (58.85%), Query Frame = 0

Query: 49  LTAAEMESLAALCDTFLPSV-------AAATSGHSNFESSAATATAAFFQTSASAAGTPE 108
           +T AEMESLA+ C+  LPSV       +     H N E     A  +F+ TS S      
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKE-----ALRSFYSTSGSKTPVLR 74

Query: 109 RVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREK 168
           +   ++++R         RL L++L+T  GT ++CG   L + +P+ + FS+LS  KRE+
Sbjct: 75  QSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRER 134

Query: 169 IALSWSLSFFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDY-PGPDPQFKLETR 228
           +      ++ L   R  F  IK+  L  FFS+V+    N +W+AI Y   PD     E +
Sbjct: 135 VLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPD-----ENK 194

Query: 229 QSEASNLNENGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASP 288
            SE  N              PL +GIV  +++      E+L   G           T   
Sbjct: 195 PSETHNER------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHD 254

Query: 289 SMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 348
           ++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EG  ++++Y + 
Sbjct: 255 AIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENG 314

Query: 349 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 408
           G+L + + + +VLAG+TVGGGS VNWSA  +TP  V +EWS+   + LF +  Y  AMEV
Sbjct: 315 GILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEV 374

Query: 409 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 468
           V K+MGV  + E E F N +LRKGCE LG++  N+PRNS   HYCG C  GC+ G KKG+
Sbjct: 375 VWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGS 434

Query: 469 AETWLVDLVRSGNGA-ILPGCEAVRIMWEK---KDGSERKRAKGITFEFGCQSGAKQMCL 528
             TWLVD V  G+GA IL GC+A R + EK     G ++ +  G+  +        +M  
Sbjct: 435 DRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAK-SLNGNIAKMLK 494

Query: 529 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 588
           +E+KVT+ A GAL TPPL+  SGLRN NIGK+LHLHPV MAWG+FP +  ++ ++     
Sbjct: 495 IEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFK---G 554

Query: 589 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 648
            SYEGGI+T++  + +E D+E     A+I+TP L PG FS+L PW SG + K RM ++SR
Sbjct: 555 NSYEGGIITSVSKVLSE-DSEV---RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSR 614

Query: 649 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 708
           TA +  + RD+GSG V++   I+Y +D  D +NL+ G+ ++LRIL AAGAEE+GTH + G
Sbjct: 615 TASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDG 674

Query: 709 RSIKVKKVSYREYEKFV----KEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRG 768
           + +  K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  G
Sbjct: 675 QRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNG 734

Query: 769 ETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTL 794
           E+WE E LF+ D+S  P+A+GVNPM+TV + AYC +  +  ++
Sbjct: 735 ESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 520.0 bits (1338), Expect = 4.7e-146
Identity = 306/774 (39.53%), Postives = 442/774 (57.11%), Query Frame = 0

Query: 32  GSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQ 91
           G P+   +     +N+  + ++++SLA +C TFLP             +S   A  +F  
Sbjct: 10  GHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPPET----------TSEQQAVNSFHV 69

Query: 92  TSASAAGTPERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTF 151
            S++     + V  MI +  R     ++R+ L +LS  +GT +LCG+  L   +P+   F
Sbjct: 70  ASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKF 129

Query: 152 SKLSHNKREKIALSWS--LSFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPG 211
           S+L  +KRE I  +WS    F L  R+ F   K  +L  FFSQ DE + N + +AI Y  
Sbjct: 130 SQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-- 189

Query: 212 PDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVA-AEALRKFGFPVAF 271
                       + +  + N +S  +E   PL +GI+       V   ++L + G  VA 
Sbjct: 190 ----------CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA- 249

Query: 272 LPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG 331
                        I+CDAVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE 
Sbjct: 250 ----RDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEV 309

Query: 332 QAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFS 391
            +M E+Y   GLL T +   ++LAGS VGGG+ VNWSAS +TP HV +EWS+  ++  F 
Sbjct: 310 PSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFG 369

Query: 392 SNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCL 451
           S  Y+ AM+ V  ++GV       GF N VLRKGCE LG    ++PRNSP DHYCG C  
Sbjct: 370 SQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGY 429

Query: 452 GCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSG 511
           GC+ G K GT +TWLVD V +G   IL G +A R +      S  +R K     F    G
Sbjct: 430 GCRAGAKNGTDQTWLVDAVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVG 489

Query: 512 AK--QMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDND 571
            K  +  ++E++VT+ + G+L TPPL+  SGL+NPNIG++L LHPV M WG+FP E D+ 
Sbjct: 490 GKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS- 549

Query: 572 KAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFK 631
               E   K YEGGI+T++ ++    D E G   A+++ P + P  ++ L PWVSG + K
Sbjct: 550 ----EFSGKMYEGGIITSVHHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLK 609

Query: 632 HRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEE 691
            RM K+ RTAH+FAL RD GSG V   + ++Y+    D ENL+ G+ +ALR+  AAGA E
Sbjct: 610 ERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVE 669

Query: 692 IGTHHNKGRSIKVKKVSYREYEKFVKEESS----RPLNDLATPICSAHQMGSCRMGIGPK 751
           +GT+ + G+ +K + ++    E+F+ E  +        +  T   SAHQMGSCRMG+  +
Sbjct: 670 VGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAE 729

Query: 752 DSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
           +  +++ GE+WE EGLF+ D S+ P+A+GVNPM+T+Q+ AYC +  ++D+L+ K
Sbjct: 730 EGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of MC11g0732 vs. NCBI nr
Match: XP_022142668.1 (long-chain-alcohol oxidase FAO4A [Momordica charantia])

HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 798/798 (100.00%), Postives = 798/798 (100.00%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL
Sbjct: 8   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 67

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR
Sbjct: 68  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 127

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT
Sbjct: 128 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 187

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 240
           IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP
Sbjct: 188 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 247

Query: 241 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 300
           LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG
Sbjct: 248 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 307

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 360
           VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS
Sbjct: 308 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 367

Query: 361 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 420
           TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR
Sbjct: 368 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 427

Query: 421 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 480
           KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA
Sbjct: 428 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 487

Query: 481 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 540
           VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN
Sbjct: 488 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 547

Query: 541 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 600
           PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG
Sbjct: 548 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 607

Query: 601 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 660
           AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM
Sbjct: 608 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 667

Query: 661 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 720
           DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN
Sbjct: 668 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 727

Query: 721 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 780
           DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 728 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 787

Query: 781 IAYCTAQSVLDTLKRKRN 798
           IAYCTAQSVLDTLKRKRN
Sbjct: 788 IAYCTAQSVLDTLKRKRN 805

BLAST of MC11g0732 vs. NCBI nr
Match: XP_038896170.1 (long-chain-alcohol oxidase FAO4A [Benincasa hispida])

HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 655/812 (80.67%), Postives = 711/812 (87.56%), Query Frame = 0

Query: 2   AASSGG----DGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNS-LTAAEMES 61
           AAS GG    DG   Q  + C++ELHGG D+  +G+ V   QHA     +S L+AAEMES
Sbjct: 18  AASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSHLSAAEMES 77

Query: 62  LAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKK 121
           LAALCDTFLPSVAA         ++A  +   FF+TSASAAGTPERVG MI ERVRHPKK
Sbjct: 78  LAALCDTFLPSVAAT--------AAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKK 137

Query: 122 WMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRM 181
           W+VR++LWMLSTWYGTYILCGN SLSNHFPYF+ FSK+S  KRE+IA+SWSLS+F L RM
Sbjct: 138 WLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRM 197

Query: 182 FFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENG-RSRE- 241
           FFRTIKIL LLAFF+QVDE+ NNISWKAIDYPG DPQFK +T+ SEASNL E G +SRE 
Sbjct: 198 FFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREI 257

Query: 242 ------EELYGPLFRGIVNID--QRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDA 301
                 EELYGPLF+GIVN+   Q+  VA  ALRKFGFPV  L   TK  +PSM+IKCDA
Sbjct: 258 DQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDA 317

Query: 302 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNM 361
           VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY +SGL+ATDNM
Sbjct: 318 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 377

Query: 362 NVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQ 421
           NVL+LAGSTVGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEAMEVVCKKMGVQ
Sbjct: 378 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 437

Query: 422 SEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDL 481
           SEI++EGFNNAVLRKGCEELG HA+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDL
Sbjct: 438 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 497

Query: 482 VRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGA 541
           VRSGNGAILPGCEA++I  EK+DGS+  RAKGI FEFGC S AKQMCLVESKVTIVACGA
Sbjct: 498 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 557

Query: 542 LSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMC 601
           L+TPPLLKRSGLRNPNIGK+LHLHPVTMAWG+FPAETD  K+WPE+ KKSYEGGIMTAMC
Sbjct: 558 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD--KSWPEKDKKSYEGGIMTAMC 617

Query: 602 NIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKG 661
           NIG+E D EPGYGGAVIQTPALHPGLFSILMPWVSGTN KHRMCKFSRTAHVFALARDKG
Sbjct: 618 NIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKG 677

Query: 662 SGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYRE 721
           SGTV+SSSS+SYKMD  DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYRE
Sbjct: 678 SGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 737

Query: 722 YEKFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVF 781
           YEKFVKEESSRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVF
Sbjct: 738 YEKFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVF 797

Query: 782 PTALGVNPMVTVQAIAYCTAQSVLDTLKRKRN 798
           PTALGVNPMVTVQAIAYCTAQS+L+TLKRKRN
Sbjct: 798 PTALGVNPMVTVQAIAYCTAQSILETLKRKRN 819

BLAST of MC11g0732 vs. NCBI nr
Match: XP_022937010.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata])

HSP 1 Score: 1279 bits (3310), Expect = 0.0
Identity = 647/803 (80.57%), Postives = 704/803 (87.67%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AAAS GGDG+ +     C++EL+GGAD++ +G P    QHA +  N SL+AAEMESLAAL
Sbjct: 17  AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF--NYSLSAAEMESLAAL 76

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAA     S F           F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77  CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           +SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S  KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
           IKIL  LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE  NL E  RSRE     E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEE 256

Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
           ELYGPLF+GIVN++ ++ VA  ALRKFGFPV  L P  K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316

Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
           GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376

Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
           VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFN 436

Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
           NAVLRKGCEELG  AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496

Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
           PGCEA+RI  EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TP LLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKR 556

Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
           SGLRNPNIGK+LHLHPVTMAWG+FPAETD  ++WPE+ KKSYEGGIMTAMCNIG+  D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616

Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
           PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676

Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
           +SY MD  DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736

Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
           SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796

Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
           VTVQAIAYCTA+SVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAKSVLETLKRKRN 802

BLAST of MC11g0732 vs. NCBI nr
Match: XP_023535226.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 645/803 (80.32%), Postives = 703/803 (87.55%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AAAS GG+G+ +     C+++LHGGAD   +G P    QHA  + N SL+AAEMESLAAL
Sbjct: 17  AAASYGGNGESKPQLHQCVIDLHGGADN--DGGPAGISQHA--NSNYSLSAAEMESLAAL 76

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAA     S +           F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77  CDTFLPSVAATDGSASEY-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           +SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S  +RE++ +SWSLS+FLL RMF+RT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQREREQVVVSWSLSYFLLLRMFYRT 196

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
           +KIL  LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE  NL E  RSRE     E
Sbjct: 197 VKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256

Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
           ELYGPLF+GIVN++ ++ VA  ALRKFGFPV  L P TK+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGG 316

Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
           GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376

Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
           VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEIE+EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIENEGFN 436

Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
           NAVLRKGCEELG  A+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496

Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
           PGCEA+RI  EK DG ER RAKG+ FEF C SGAKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRERNRAKGVAFEFECGSGAKQMCLVESKVTIVACGALCTPPLLKR 556

Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
           SGLRNPNIGK+LHLHPVTMAWG+FPAETD  ++WPE+ KKSYEGGIMTAMCNIG+  D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616

Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
           PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676

Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
           +SY MD  DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736

Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
           SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796

Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
           VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 800

BLAST of MC11g0732 vs. NCBI nr
Match: KAG6591858.1 (Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1268 bits (3280), Expect = 0.0
Identity = 640/803 (79.70%), Postives = 700/803 (87.17%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           A AS GGDG+ +     C++EL+GGAD+  +G P    QHA  + N SL+AAEMESLAAL
Sbjct: 17  ATASYGGDGESKPQLHQCVIELNGGADSRYDGGPAGISQHA--NSNYSLSAAEMESLAAL 76

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAA     S F           F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77  CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           +SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S  KRE++ +SWSLS+FLL +MFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLKMFFRT 196

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
           IKIL  LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE  NL E  RSRE     E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256

Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
           ELYGPLF+GIVN++ ++ VA  ALRKFGFPV  L P  K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316

Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
           GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376

Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
           VGGGSTVNWSAS QTP+HVTREWS  YEL+LF SNLYKEA+EVVCK+MGVQSE+++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSGEYELELFGSNLYKEALEVVCKRMGVQSEMDNEGFN 436

Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
           NA+LRKGCEELG  A+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAILRKGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496

Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
           PGCEA+RI  EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPPLLKR 556

Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
           SGL NPNIGK+LHLHPV MAWG+FPAETD  ++WPE+ KKSYEGGIMTAMCNIG+  D E
Sbjct: 557 SGLTNPNIGKNLHLHPVAMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616

Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
           PGYGGAVIQTPALHPGLFSILMPWVSGTN K +MCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQKMCKFSRTAHVFALARDKGSGTVESSSS 676

Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
           +SY MD  DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736

Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
           SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796

Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
           VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 802

BLAST of MC11g0732 vs. ExPASy TrEMBL
Match: A0A6J1CNU3 (Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 SV=1)

HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 798/798 (100.00%), Postives = 798/798 (100.00%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL
Sbjct: 8   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 67

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR
Sbjct: 68  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 127

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT
Sbjct: 128 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 187

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 240
           IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP
Sbjct: 188 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 247

Query: 241 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 300
           LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG
Sbjct: 248 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 307

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 360
           VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS
Sbjct: 308 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 367

Query: 361 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 420
           TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR
Sbjct: 368 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 427

Query: 421 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 480
           KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA
Sbjct: 428 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 487

Query: 481 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 540
           VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN
Sbjct: 488 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 547

Query: 541 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 600
           PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG
Sbjct: 548 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 607

Query: 601 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 660
           AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM
Sbjct: 608 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 667

Query: 661 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 720
           DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN
Sbjct: 668 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 727

Query: 721 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 780
           DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 728 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 787

Query: 781 IAYCTAQSVLDTLKRKRN 798
           IAYCTAQSVLDTLKRKRN
Sbjct: 788 IAYCTAQSVLDTLKRKRN 805

BLAST of MC11g0732 vs. ExPASy TrEMBL
Match: A0A6J1FFC0 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV=1)

HSP 1 Score: 1279 bits (3310), Expect = 0.0
Identity = 647/803 (80.57%), Postives = 704/803 (87.67%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AAAS GGDG+ +     C++EL+GGAD++ +G P    QHA +  N SL+AAEMESLAAL
Sbjct: 17  AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF--NYSLSAAEMESLAAL 76

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           CDTFLPSVAA     S F           F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77  CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           +SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S  KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
           IKIL  LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE  NL E  RSRE     E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEE 256

Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
           ELYGPLF+GIVN++ ++ VA  ALRKFGFPV  L P  K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316

Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
           GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376

Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
           VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFN 436

Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
           NAVLRKGCEELG  AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496

Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
           PGCEA+RI  EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TP LLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKR 556

Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
           SGLRNPNIGK+LHLHPVTMAWG+FPAETD  ++WPE+ KKSYEGGIMTAMCNIG+  D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616

Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
           PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676

Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
           +SY MD  DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736

Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
           SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796

Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
           VTVQAIAYCTA+SVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAKSVLETLKRKRN 802

BLAST of MC11g0732 vs. ExPASy TrEMBL
Match: A0A6J1IPF0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1)

HSP 1 Score: 1267 bits (3278), Expect = 0.0
Identity = 642/803 (79.95%), Postives = 700/803 (87.17%), Query Frame = 0

Query: 1   AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
           AA+S GGDG+ +     C++EL+G AD++ +G P    QHA  + N SL+AAEMESLAAL
Sbjct: 17  AASSYGGDGESKPQLHQCVIELNGCADSLHDGGPAGISQHA--NSNYSLSAAEMESLAAL 76

Query: 61  CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
           C+TFLPSVAA     S F           F+TSASAAGT ERVGGMI ERVRHPKKW++R
Sbjct: 77  CETFLPSVAATDGSASEF-----------FETSASAAGTSERVGGMIKERVRHPKKWLMR 136

Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
           +SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S  KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 MSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196

Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
           IKILS LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE  NL E  RSRE     E
Sbjct: 197 IKILSHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256

Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
           ELYGPLF+GIVN++ +  VA  ALRKFGFPV  L P TK+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQV-VAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGG 316

Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
           GVV GVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVGGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376

Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
           VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIDNEGFN 436

Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
           NA+LRKGCEELG  AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAILRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496

Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
           PGCEA+RI  EK DG ER RAKG+ FEF C S AKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRERNRAKGVAFEFECGSEAKQMCLVESKVTIVACGALCTPPLLKR 556

Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
           SGLRNPNIGK+LHLHPV MAWG+FPAETD  ++WPE+ KKSYEGGIMTAMCNIG+  D E
Sbjct: 557 SGLRNPNIGKNLHLHPVAMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616

Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
           PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676

Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
           +SY MD  DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736

Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
           SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLF+ADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFVADSSVFPTALGVNPM 796

Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
           VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 802

BLAST of MC11g0732 vs. ExPASy TrEMBL
Match: A0A1S3BAP1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1)

HSP 1 Score: 1266 bits (3275), Expect = 0.0
Identity = 644/815 (79.02%), Postives = 704/815 (86.38%), Query Frame = 0

Query: 1   AAASSGGDGDGE---QHRRHCIVELHGGADTILNGSPVQNPQHAK-----YSHNNSLTAA 60
           AAA+ GG  DGE   Q   HC++ELHGGAD+  +G+ V+  QHA      YS    L+AA
Sbjct: 17  AAANGGGGDDGETNHQPHHHCVIELHGGADSFHDGASVEISQHANSNNDYYSSAGHLSAA 76

Query: 61  EMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVR 120
           EMESLAALCDTFLPSVA AT+   +        TA FF+TSASA GTPERVG MI ERVR
Sbjct: 77  EMESLAALCDTFLPSVAPATAADRS--------TAVFFETSASAVGTPERVGEMIMERVR 136

Query: 121 HPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFL 180
           HPKKW+VR+SLWMLSTWYGTYILCGN SLSNHFPYF+ FSK+S  KRE+IALSWSLS+F 
Sbjct: 137 HPKKWLVRVSLWMLSTWYGTYILCGNESLSNHFPYFKRFSKVSQKKREQIALSWSLSYFF 196

Query: 181 LHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFK----LETRQSEASNLNE 240
           L RMFFRTIKIL LLAFF+QVDE+MNNISWKAIDYPG DPQFK    L+ + S++  ++E
Sbjct: 197 LLRMFFRTIKILCLLAFFTQVDEEMNNISWKAIDYPGSDPQFKPPSNLKEKDSKSREVDE 256

Query: 241 NGRSREEE---LYGPLFRGIVNID--QRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIK 300
            G   EEE   LYGPLF+GIVN+   Q+  VA  ALRKFGFPV  L P TK  +PSM+IK
Sbjct: 257 EGDEEEEEAEELYGPLFKGIVNLQHQQQIQVAVGALRKFGFPVTLLSPTTKPPNPSMIIK 316

Query: 301 CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLAT 360
           CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY SSGL+AT
Sbjct: 317 CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQSSGLVAT 376

Query: 361 DNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKM 420
           DNMNVL+LAGSTVGGGSTVNWSAS +TP+HVT+EWSD YEL+LF SNLYKEA++VVCKKM
Sbjct: 377 DNMNVLILAGSTVGGGSTVNWSASIKTPSHVTKEWSDEYELELFRSNLYKEAIDVVCKKM 436

Query: 421 GVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWL 480
           GVQS+I++EGFNNAVLRKGCEELG HAINIPRNS +DHYCGWCCLGC++GRKKGTAETWL
Sbjct: 437 GVQSKIDNEGFNNAVLRKGCEELGGHAINIPRNSTSDHYCGWCCLGCRDGRKKGTAETWL 496

Query: 481 VDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVA 540
           VDLVRSGNG ILPGCEA++I  EKK G ERK AKG+ FEF C S AKQMCLVESKVTIVA
Sbjct: 497 VDLVRSGNGVILPGCEAIKIRQEKKQGRERKMAKGVAFEFWCGSAAKQMCLVESKVTIVA 556

Query: 541 CGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMT 600
           CGAL+TPPLLKRSGLRNPNIGK+LHLHPVTMAWG+FPAETD  K+WPE+ KKSYEGGIMT
Sbjct: 557 CGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD--KSWPEKDKKSYEGGIMT 616

Query: 601 AMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALAR 660
           AMCNIG E + EPGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVF LAR
Sbjct: 617 AMCNIGPEPNQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFVLAR 676

Query: 661 DKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVS 720
           DKGSGTV+S SS+SYKMD  DE+NLQKG+EKAL+ILAAAGA EIGTHHNKGRSI VKKVS
Sbjct: 677 DKGSGTVKSPSSLSYKMDNVDEQNLQKGLEKALKILAAAGAVEIGTHHNKGRSINVKKVS 736

Query: 721 YREYEKFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADS 780
           YREYEKFVKEESSRPL DL TPICSAHQMGSCRMG G KDSVVNQRGETWE+EGLFIADS
Sbjct: 737 YREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGSGAKDSVVNQRGETWEVEGLFIADS 796

Query: 781 SVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRKRN 798
           SVFPTALGVNPMVTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 SVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKRN 821

BLAST of MC11g0732 vs. ExPASy TrEMBL
Match: A0A6J1FQT3 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV=1)

HSP 1 Score: 1243 bits (3216), Expect = 0.0
Identity = 635/798 (79.57%), Postives = 689/798 (86.34%), Query Frame = 0

Query: 6   GGDGDGEQHRR--HCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAALCDT 65
           GG GD E  R+  H ++ELH GA       P +  +HA  ++N SL+AAEMESLAALCDT
Sbjct: 54  GGGGDDEAKRKLDHYVIELHDGA-------PAEILEHANSNNNCSLSAAEMESLAALCDT 113

Query: 66  FLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVRLSL 125
           FLPSV A  S             A FF+TSA+AAGTPE+VG MI ERV+HPKKW+VR+SL
Sbjct: 114 FLPSVTADGSA------------AMFFETSAAAAGTPEKVGKMIKERVKHPKKWLVRVSL 173

Query: 126 WMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRTIKI 185
           WMLSTWYGTYILCGN SLS++FPYFQTF KLS  KRE+I +SWSLS+F L RM+FRTIKI
Sbjct: 174 WMLSTWYGTYILCGNRSLSHNFPYFQTFPKLSQTKREQIVVSWSLSYFFLLRMWFRTIKI 233

Query: 186 LSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGPLFR 245
             LL FF+QVD+K NNISW+AIDYPG DPQFK +T+Q EAS   E     EEELYGPLF+
Sbjct: 234 FCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEASREVEED---EEELYGPLFK 293

Query: 246 GIVNIDQRR---GVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 305
           GIV++D ++     AAEALRKFGFPV  L P  K +SPSM+IKCDAVVVGSGSGGGVVAG
Sbjct: 294 GIVHLDNQQVAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKCDAVVVGSGSGGGVVAG 353

Query: 306 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 365
           VLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVLVLAGSTVGGGS
Sbjct: 354 VLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 413

Query: 366 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 425
           TVNWSAS QTP+HVTREWSD YEL+LFSSNLYKEAMEVVCKKMGVQSEI++EGFNNAVLR
Sbjct: 414 TVNWSASIQTPSHVTREWSDKYELELFSSNLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 473

Query: 426 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 485
           KGCEELGYHAINIPRNS +DHYCGWCCLGCK+GRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 474 KGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLVDLVRSGNGVILPGCEA 533

Query: 486 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 545
           + I  EKKDGSER RAKGI FEFGC SG KQ+CLVESKVTIVACGALSTPPLL RSGLRN
Sbjct: 534 IDIRKEKKDGSERNRAKGIAFEFGCGSGVKQVCLVESKVTIVACGALSTPPLLLRSGLRN 593

Query: 546 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 605
           PNIGK+LHLHPVTMAWGHFPAETD  K WPEE KKSYEGGIMTAMCNI +E D EPG GG
Sbjct: 594 PNIGKNLHLHPVTMAWGHFPAETD--KPWPEEHKKSYEGGIMTAMCNIRSEPDQEPGSGG 653

Query: 606 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 665
           AVIQTPALHPGLFSILMPW+SGTN K RM KFSRTAHVF LARDKGSGTV+S + ISY M
Sbjct: 654 AVIQTPALHPGLFSILMPWLSGTNIKQRMRKFSRTAHVFVLARDKGSGTVKSPNCISYNM 713

Query: 666 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 725
           +  DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYREYEKFVKEESSR L 
Sbjct: 714 EEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRALK 773

Query: 726 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 785
           DL TPICSAHQMGSCRMGIG +DSVV+QRGETWE+EGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 774 DLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVFPTALGVNPMVTVQA 827

Query: 786 IAYCTAQSVLDTLKRKRN 798
           IAYCTAQSVL+TLKRKRN
Sbjct: 834 IAYCTAQSVLETLKRKRN 827

BLAST of MC11g0732 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 863.2 bits (2229), Expect = 1.6e-250
Identity = 436/747 (58.37%), Postives = 556/747 (74.43%), Query Frame = 0

Query: 54  MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 113
           MESL A+CDTF+ S+  +  GH +         A +F  SAS  GTP+RV  ++SER+ H
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVD------DCVAGYFSASASQTGTPDRVARLMSERLHH 60

Query: 114 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 173
           PKKW++R  LW+LSTW G+ +LCG  S +  FPYF+ F +L   +RE+I L+WS S+F L
Sbjct: 61  PKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSL 120

Query: 174 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 233
            RM FRTIK++S L FF+QVDEK  N++WKAI Y GP P    +    E     E  + +
Sbjct: 121 LRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSP----DHSDHEVELNEEKKKKK 180

Query: 234 EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTAS---PSMMIKCDAVVVG 293
            EE++GPL+ GIV++   R    + L   GF V+     T  +S   P M I+CDAVVVG
Sbjct: 181 PEEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVG 240

Query: 294 SGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLV 353
           SGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GLLAT + NV++
Sbjct: 241 SGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVI 300

Query: 354 LAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIE 413
           LAGSTVGGGST+NWSAS +TP HV +EW++  +L++F S+LY+EAM+VVCK+MGVQ    
Sbjct: 301 LAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFV 360

Query: 414 SEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSG 473
            EGFNN VLRKGCE+LG    NIPRN+P+DHYCG+CCLGCK G+K+GT+ETWLVDLV S 
Sbjct: 361 EEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESD 420

Query: 474 NGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTP 533
           NG ILPGC+A  +M++ + G ++K+A G+ F FG     +++ +VES+VTIVACGAL TP
Sbjct: 421 NGLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTP 480

Query: 534 PLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGT 593
            LLKRSGL+N NIG++L LHPV MAWG FP E   DK WPE+ KKSYEGGIMTAM ++  
Sbjct: 481 HLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEE---DK-WPEKKKKSYEGGIMTAMSSVVI 540

Query: 594 EADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTV 653
           E +    YG  VIQTPALHPG+FS ++PW S  +FK RM KFSRTAH+FAL RDKG+GT+
Sbjct: 541 E-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTI 600

Query: 654 RSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKF 713
            S + I Y ++  DEE+L+ G+E+ L+ILAAAGAEEIGTHH++GRS+ V+  S  E E+F
Sbjct: 601 DSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERF 660

Query: 714 VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTAL 773
           V+EESS+PL DL+  ICSAHQMGSCRMGI P++S V   GETWE+E LF+AD+SVFPTAL
Sbjct: 661 VREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTAL 720

Query: 774 GVNPMVTVQAIAYCTAQSVLDTLKRKR 798
           GVNPMVTVQ+IAYC   +V+D LK+K+
Sbjct: 721 GVNPMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of MC11g0732 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 525.4 bits (1352), Expect = 8.0e-149
Identity = 313/763 (41.02%), Postives = 449/763 (58.85%), Query Frame = 0

Query: 49  LTAAEMESLAALCDTFLPSV-------AAATSGHSNFESSAATATAAFFQTSASAAGTPE 108
           +T AEMESLA+ C+  LPSV       +     H N E     A  +F+ TS S      
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKE-----ALRSFYSTSGSKTPVLR 74

Query: 109 RVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREK 168
           +   ++++R         RL L++L+T  GT ++CG   L + +P+ + FS+LS  KRE+
Sbjct: 75  QSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRER 134

Query: 169 IALSWSLSFFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDY-PGPDPQFKLETR 228
           +      ++ L   R  F  IK+  L  FFS+V+    N +W+AI Y   PD     E +
Sbjct: 135 VLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPD-----ENK 194

Query: 229 QSEASNLNENGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASP 288
            SE  N              PL +GIV  +++      E+L   G           T   
Sbjct: 195 PSETHNER------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHD 254

Query: 289 SMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 348
           ++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EG  ++++Y + 
Sbjct: 255 AIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENG 314

Query: 349 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 408
           G+L + + + +VLAG+TVGGGS VNWSA  +TP  V +EWS+   + LF +  Y  AMEV
Sbjct: 315 GILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEV 374

Query: 409 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 468
           V K+MGV  + E E F N +LRKGCE LG++  N+PRNS   HYCG C  GC+ G KKG+
Sbjct: 375 VWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGS 434

Query: 469 AETWLVDLVRSGNGA-ILPGCEAVRIMWEK---KDGSERKRAKGITFEFGCQSGAKQMCL 528
             TWLVD V  G+GA IL GC+A R + EK     G ++ +  G+  +        +M  
Sbjct: 435 DRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAK-SLNGNIAKMLK 494

Query: 529 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 588
           +E+KVT+ A GAL TPPL+  SGLRN NIGK+LHLHPV MAWG+FP +  ++ ++     
Sbjct: 495 IEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFK---G 554

Query: 589 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 648
            SYEGGI+T++  + +E D+E     A+I+TP L PG FS+L PW SG + K RM ++SR
Sbjct: 555 NSYEGGIITSVSKVLSE-DSEV---RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSR 614

Query: 649 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 708
           TA +  + RD+GSG V++   I+Y +D  D +NL+ G+ ++LRIL AAGAEE+GTH + G
Sbjct: 615 TASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDG 674

Query: 709 RSIKVKKVSYREYEKFV----KEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRG 768
           + +  K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  G
Sbjct: 675 QRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNG 734

Query: 769 ETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTL 794
           E+WE E LF+ D+S  P+A+GVNPM+TV + AYC +  +  ++
Sbjct: 735 ESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of MC11g0732 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 3.4e-147
Identity = 306/774 (39.53%), Postives = 442/774 (57.11%), Query Frame = 0

Query: 32  GSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQ 91
           G P+   +     +N+  + ++++SLA +C TFLP             +S   A  +F  
Sbjct: 10  GHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPPET----------TSEQQAVNSFHV 69

Query: 92  TSASAAGTPERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTF 151
            S++     + V  MI +  R     ++R+ L +LS  +GT +LCG+  L   +P+   F
Sbjct: 70  ASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKF 129

Query: 152 SKLSHNKREKIALSWS--LSFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPG 211
           S+L  +KRE I  +WS    F L  R+ F   K  +L  FFSQ DE + N + +AI Y  
Sbjct: 130 SQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-- 189

Query: 212 PDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVA-AEALRKFGFPVAF 271
                       + +  + N +S  +E   PL +GI+       V   ++L + G  VA 
Sbjct: 190 ----------CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA- 249

Query: 272 LPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG 331
                        I+CDAVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE 
Sbjct: 250 ----RDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEV 309

Query: 332 QAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFS 391
            +M E+Y   GLL T +   ++LAGS VGGG+ VNWSAS +TP HV +EWS+  ++  F 
Sbjct: 310 PSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFG 369

Query: 392 SNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCL 451
           S  Y+ AM+ V  ++GV       GF N VLRKGCE LG    ++PRNSP DHYCG C  
Sbjct: 370 SQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGY 429

Query: 452 GCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSG 511
           GC+ G K GT +TWLVD V +G   IL G +A R +      S  +R K     F    G
Sbjct: 430 GCRAGAKNGTDQTWLVDAVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVG 489

Query: 512 AK--QMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDND 571
            K  +  ++E++VT+ + G+L TPPL+  SGL+NPNIG++L LHPV M WG+FP E D+ 
Sbjct: 490 GKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS- 549

Query: 572 KAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFK 631
               E   K YEGGI+T++ ++    D E G   A+++ P + P  ++ L PWVSG + K
Sbjct: 550 ----EFSGKMYEGGIITSVHHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLK 609

Query: 632 HRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEE 691
            RM K+ RTAH+FAL RD GSG V   + ++Y+    D ENL+ G+ +ALR+  AAGA E
Sbjct: 610 ERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVE 669

Query: 692 IGTHHNKGRSIKVKKVSYREYEKFVKEESS----RPLNDLATPICSAHQMGSCRMGIGPK 751
           +GT+ + G+ +K + ++    E+F+ E  +        +  T   SAHQMGSCRMG+  +
Sbjct: 670 VGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAE 729

Query: 752 DSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
           +  +++ GE+WE EGLF+ D S+ P+A+GVNPM+T+Q+ AYC +  ++D+L+ K
Sbjct: 730 EGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of MC11g0732 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-140
Identity = 291/788 (36.93%), Postives = 452/788 (57.36%), Query Frame = 0

Query: 24  GGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSG-HSNFESSA 83
           GG      GSP+      + S ++  + +++++L+++CD  +P V   +       +   
Sbjct: 3   GGRRLGKRGSPLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLR 62

Query: 84  ATATAAFFQTSASAAGT-PERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASL 143
             A  +FF++S+S +   P+ V  +++ +       +VR+ L +L+   GT +LCG   L
Sbjct: 63  NDALLSFFKSSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCL 122

Query: 144 -SNHFPYFQTFSKLSHNKREKIALSWSLSFF-LLHRMFFRTIKILSLLAFFSQVDEKMNN 203
              H+P+   FS++S  KREK+   W+  ++  L R+ F  IK + L  +F+  +E   N
Sbjct: 123 DKKHWPFLLKFSEMSLEKREKVLQRWNTQWYNPLARIGFMMIKAIFLFYYFTWTNENSEN 182

Query: 204 ISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVAAEAL 263
             W AI+Y               +  + EN    ++E   PL  GI+   +   +  +  
Sbjct: 183 PVWDAINY---------------SVEIGENEDMEQKE--RPLDEGIIETAKEDEMTIKQ- 242

Query: 264 RKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCA 323
           R     +     R +    +  I+CDAVVVGSG GGGV A +LA +G +V+V+EKG+Y A
Sbjct: 243 RMINKGLKVTEDRER---DTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFA 302

Query: 324 RTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWS 383
             + S LEG +M E++ S+ L+ T +     +AGSTVGGGS VNW+AS +TP  +  EWS
Sbjct: 303 PRDYSALEGPSMFELFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWS 362

Query: 384 DVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPA 443
               + ++SS  YK AM +VCK++GV  +I  EGF N +LRKGCE+LG     +PRNS  
Sbjct: 363 VHRGISIYSSEKYKAAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTE 422

Query: 444 DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMW------EKKDGSER 503
            HYCG C  GC  G K+GT  TWLVD V + N  IL  C+A +++       ++++   R
Sbjct: 423 KHYCGSCSYGCPTGEKRGTDSTWLVDAV-NNNAVILTQCKAEKLILADNDANKREESGRR 482

Query: 504 KRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVT 563
           KR  G+      Q+  K    + +KVTIVACG+L TP LL  SGL+N NI + LH+HP+ 
Sbjct: 483 KRCLGVAASLSHQTRKKLQ--INAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHPIM 542

Query: 564 MAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLF 623
           MAWG+FP +        E    ++EG I+T++  +       P      ++TPA+ PG F
Sbjct: 543 MAWGYFPEKNS------ELEGAAHEGEIVTSLHYVHPMDSTTP---NITLETPAIGPGTF 602

Query: 624 SILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVE 683
           + L PWVSG++ K RM K++RTAH+FA+ RD+G G V+    + Y++  ADEENL  G++
Sbjct: 603 AALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVK-GDIVKYRLTKADEENLTIGLK 662

Query: 684 KALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRP----LNDLATPICSA 743
           +ALRIL AAGA E+GT+ + G+ +K   +  ++ E F+   ++ P    ++   T   +A
Sbjct: 663 QALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTA 722

Query: 744 HQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSV 798
           HQ+G CRMG   K+  ++ +GE+WE E L++ D+SV PTALGVNPM+TVQ+ AYC +  +
Sbjct: 723 HQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRI 756