Homology
BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match:
O65709 (Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 SV=2)
HSP 1 Score: 863.2 bits (2229), Expect = 2.3e-249
Identity = 436/747 (58.37%), Postives = 556/747 (74.43%), Query Frame = 0
Query: 54 MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 113
MESL A+CDTF+ S+ + GH + A +F SAS GTP+RV ++SER+ H
Sbjct: 1 MESLVAICDTFISSIDDSGVGHVD------DCVAGYFSASASQTGTPDRVARLMSERLHH 60
Query: 114 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 173
PKKW++R LW+LSTW G+ +LCG S + FPYF+ F +L +RE+I L+WS S+F L
Sbjct: 61 PKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSL 120
Query: 174 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 233
RM FRTIK++S L FF+QVDEK N++WKAI Y GP P + E E + +
Sbjct: 121 LRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSP----DHSDHEVELNEEKKKKK 180
Query: 234 EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTAS---PSMMIKCDAVVVG 293
EE++GPL+ GIV++ R + L GF V+ T +S P M I+CDAVVVG
Sbjct: 181 PEEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVG 240
Query: 294 SGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLV 353
SGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GLLAT + NV++
Sbjct: 241 SGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVI 300
Query: 354 LAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIE 413
LAGSTVGGGST+NWSAS +TP HV +EW++ +L++F S+LY+EAM+VVCK+MGVQ
Sbjct: 301 LAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFV 360
Query: 414 SEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSG 473
EGFNN VLRKGCE+LG NIPRN+P+DHYCG+CCLGCK G+K+GT+ETWLVDLV S
Sbjct: 361 EEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESD 420
Query: 474 NGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTP 533
NG ILPGC+A +M++ + G ++K+A G+ F FG +++ +VES+VTIVACGAL TP
Sbjct: 421 NGLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTP 480
Query: 534 PLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGT 593
LLKRSGL+N NIG++L LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++
Sbjct: 481 HLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEE---DK-WPEKKKKSYEGGIMTAMSSVVI 540
Query: 594 EADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTV 653
E + YG VIQTPALHPG+FS ++PW S +FK RM KFSRTAH+FAL RDKG+GT+
Sbjct: 541 E-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTI 600
Query: 654 RSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKF 713
S + I Y ++ DEE+L+ G+E+ L+ILAAAGAEEIGTHH++GRS+ V+ S E E+F
Sbjct: 601 DSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERF 660
Query: 714 VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTAL 773
V+EESS+PL DL+ ICSAHQMGSCRMGI P++S V GETWE+E LF+AD+SVFPTAL
Sbjct: 661 VREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTAL 720
Query: 774 GVNPMVTVQAIAYCTAQSVLDTLKRKR 798
GVNPMVTVQ+IAYC +V+D LK+K+
Sbjct: 721 GVNPMVTVQSIAYCIGLNVVDVLKKKK 726
BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match:
B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)
HSP 1 Score: 564.7 bits (1454), Expect = 1.7e-159
Identity = 316/755 (41.85%), Postives = 453/755 (60.00%), Query Frame = 0
Query: 50 TAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISE 109
+++EMESLA++C+ LP + + + F+ TSAS P V ++S+
Sbjct: 23 SSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNTSASHYPIPHEVAEILSK 82
Query: 110 RVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLS 169
R ++R+ LW+L+T GT++LCG S +P+ FS +S KRE++ W +
Sbjct: 83 RSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFSSMSLKKRERVMQWWLKN 142
Query: 170 FFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNE 229
F+ R+ F +K+L L A+F+ VDE +N +WKAI Y E E +
Sbjct: 143 RFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGY---------EVSADEKLPNSS 202
Query: 230 NGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAV 289
NGR E +GIV + + ++L + G V + + + +KCDA+
Sbjct: 203 NGRLLE--------KGIVETMHETNSTLQQSLTEKGLNVTL-----DSRNNILKVKCDAI 262
Query: 290 VVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMN 349
VVGSG GGGV A VL+ AG+KV+VLEKG+Y A + S LEG +M+++Y + G+LA+ +
Sbjct: 263 VVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVDSR 322
Query: 350 VLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQS 409
+L+LAGSTVGGGS VNWSA +TP V +EWS+ + L LFS++ Y AME VC+++GV
Sbjct: 323 ILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGVTE 382
Query: 410 EIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLV 469
EGF N VLRKGC+ LG +PRNS +H+CG C GC G K+GT +TWLVD V
Sbjct: 383 SCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVDAV 442
Query: 470 RSGNGAILPGCEAVRIMWE-KKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGA 529
+G I+ GC+A R + E ++GS RK+ S +E+KVTI A GA
Sbjct: 443 ENG-AVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGGA 502
Query: 530 LSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMC 589
L TPPL+ SGL+N NIGK+LHLHPV M WG+FP T K K +EGGI+T++
Sbjct: 503 LLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKG------KIFEGGIITSVH 562
Query: 590 NIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKG 649
+ + N A+I+TP+L PG F+ L PW SG +FK RM + RTAH+ + RDK
Sbjct: 563 KVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKA 622
Query: 650 SGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYRE 709
G V + +SYK+ D+EN++ G+++ALRIL AAGA E+GTH + G+ +K +VS E
Sbjct: 623 CGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKCGEVSENE 682
Query: 710 YEKFVKEESSRPLNDLATP------ICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFI 769
+F+ +S P+ +P SAHQMGSCRMG+ K+ V++ GE+WE +GLF+
Sbjct: 683 MAEFI--DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESWEAQGLFV 742
Query: 770 ADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKR 796
D+S+ PTA+GVNPM+T+Q+ AYC + V+D LKR
Sbjct: 743 CDASLLPTAVGVNPMITIQSTAYCISNRVVDYLKR 746
BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match:
B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)
HSP 1 Score: 538.9 bits (1387), Expect = 9.8e-152
Identity = 316/768 (41.15%), Postives = 460/768 (59.90%), Query Frame = 0
Query: 43 YSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPER 102
YSH L++++M +AA+C+ PS + N +SS A +AF+ S S A P+
Sbjct: 23 YSH--GLSSSQMHVIAAICEALFPSQPLDS---QNNQSSVDKALSAFYTASGSQAPLPDE 82
Query: 103 VGGMISERVRHPKKWMVRLS--LWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKRE 162
++ + R + + +S L +LS GT +LCG L +P+ FS++ KRE
Sbjct: 83 AAELLFKFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKFSEIPLEKRE 142
Query: 163 KIALSWSL-SFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETR 222
+I WS ++ R+ F K++ FS+ D +N WKAI Y +++TR
Sbjct: 143 EILKRWSREKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGY-------QVDTR 202
Query: 223 QSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPS 282
+ L + R +E L ++ + ++L + G V T+ +
Sbjct: 203 E----KLTQKKRPLQEGLIETMY-------ETDSTLIQSLTEKGLEV------TEDLEQN 262
Query: 283 M-MIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 342
M IKCDAV+VGSG GGGV A VLAN+G+KV++LEKG Y + S LEG +M E+Y S
Sbjct: 263 MYKIKCDAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESG 322
Query: 343 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 402
G+L + + +++LAGST+GG S +NWSA +TP V REWS+ +++ LF+S Y+ AM+
Sbjct: 323 GILPSLDGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDT 382
Query: 403 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 462
VC+++GV E F N +LR+GC ++G+ + NS ADHYCG CC GC+ G KKGT
Sbjct: 383 VCRRIGVTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGT 442
Query: 463 AETWLVDLVRSGNGA-ILPGCEAVRIMWEKKD---GSERKRAKGITFEFGCQSGAKQMCL 522
TWLVD V GNGA IL GC+A ++ + KD G++RK G+ +S +
Sbjct: 443 ESTWLVDAV--GNGAVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSKVTKKLQ 502
Query: 523 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 582
+ESKVTI ACG+LSTPPL+ SGL+NPNIGK+LHLHP AWG+FP + N
Sbjct: 503 IESKVTISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSG------ 562
Query: 583 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 642
+YEGGI+T++ + E + +I+ PAL PG FS L+PWVSG + K RM K++R
Sbjct: 563 NNYEGGIITSIHKVFEE--DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYAR 622
Query: 643 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 702
TA++FAL RD GSG V++ ISYK+D D E+LQ G+ KALRIL AAGA E+GT+ + G
Sbjct: 623 TANLFALVRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDG 682
Query: 703 RSIKVKKVSYREYEKF------VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQ 762
+ IK + + + E+F V SSR N++ T SAHQM SCRM ++ V++
Sbjct: 683 QRIKCRGIKESDLEEFLDSVRVVGGPSSR--NEVWTVFTSAHQMTSCRMSATEEEGAVDE 742
Query: 763 RGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
GE+WE +GL++ D SV P+A+GVNPM+T+Q+ AYC A ++ ++LK++
Sbjct: 743 NGESWEAKGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKKQ 749
BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match:
Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)
HSP 1 Score: 525.4 bits (1352), Expect = 1.1e-147
Identity = 313/763 (41.02%), Postives = 449/763 (58.85%), Query Frame = 0
Query: 49 LTAAEMESLAALCDTFLPSV-------AAATSGHSNFESSAATATAAFFQTSASAAGTPE 108
+T AEMESLA+ C+ LPSV + H N E A +F+ TS S
Sbjct: 15 ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKE-----ALRSFYSTSGSKTPVLR 74
Query: 109 RVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREK 168
+ ++++R RL L++L+T GT ++CG L + +P+ + FS+LS KRE+
Sbjct: 75 QSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRER 134
Query: 169 IALSWSLSFFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDY-PGPDPQFKLETR 228
+ ++ L R F IK+ L FFS+V+ N +W+AI Y PD E +
Sbjct: 135 VLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPD-----ENK 194
Query: 229 QSEASNLNENGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASP 288
SE N PL +GIV +++ E+L G T
Sbjct: 195 PSETHNER------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHD 254
Query: 289 SMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 348
++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY + EG ++++Y +
Sbjct: 255 AIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENG 314
Query: 349 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 408
G+L + + + +VLAG+TVGGGS VNWSA +TP V +EWS+ + LF + Y AMEV
Sbjct: 315 GILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEV 374
Query: 409 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 468
V K+MGV + E E F N +LRKGCE LG++ N+PRNS HYCG C GC+ G KKG+
Sbjct: 375 VWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGS 434
Query: 469 AETWLVDLVRSGNGA-ILPGCEAVRIMWEK---KDGSERKRAKGITFEFGCQSGAKQMCL 528
TWLVD V G+GA IL GC+A R + EK G ++ + G+ + +M
Sbjct: 435 DRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAK-SLNGNIAKMLK 494
Query: 529 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 588
+E+KVT+ A GAL TPPL+ SGLRN NIGK+LHLHPV MAWG+FP + ++ ++
Sbjct: 495 IEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFK---G 554
Query: 589 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 648
SYEGGI+T++ + +E D+E A+I+TP L PG FS+L PW SG + K RM ++SR
Sbjct: 555 NSYEGGIITSVSKVLSE-DSEV---RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSR 614
Query: 649 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 708
TA + + RD+GSG V++ I+Y +D D +NL+ G+ ++LRIL AAGAEE+GTH + G
Sbjct: 615 TASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDG 674
Query: 709 RSIKVKKVSYREYEKFV----KEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRG 768
+ + K V+ ++F+ EE ++ + + SAHQMGSCR+G K+ ++ G
Sbjct: 675 QRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNG 734
Query: 769 ETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTL 794
E+WE E LF+ D+S P+A+GVNPM+TV + AYC + + ++
Sbjct: 735 ESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740
BLAST of MC11g0732 vs. ExPASy Swiss-Prot
Match:
Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)
HSP 1 Score: 520.0 bits (1338), Expect = 4.7e-146
Identity = 306/774 (39.53%), Postives = 442/774 (57.11%), Query Frame = 0
Query: 32 GSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQ 91
G P+ + +N+ + ++++SLA +C TFLP +S A +F
Sbjct: 10 GHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPPET----------TSEQQAVNSFHV 69
Query: 92 TSASAAGTPERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTF 151
S++ + V MI + R ++R+ L +LS +GT +LCG+ L +P+ F
Sbjct: 70 ASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKF 129
Query: 152 SKLSHNKREKIALSWS--LSFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPG 211
S+L +KRE I +WS F L R+ F K +L FFSQ DE + N + +AI Y
Sbjct: 130 SQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-- 189
Query: 212 PDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVA-AEALRKFGFPVAF 271
+ + + N +S +E PL +GI+ V ++L + G VA
Sbjct: 190 ----------CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA- 249
Query: 272 LPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG 331
I+CDAVVVGSGSGGGV A LA AG KVLVLEKG+Y + S LE
Sbjct: 250 ----RDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEV 309
Query: 332 QAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFS 391
+M E+Y GLL T + ++LAGS VGGG+ VNWSAS +TP HV +EWS+ ++ F
Sbjct: 310 PSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFG 369
Query: 392 SNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCL 451
S Y+ AM+ V ++GV GF N VLRKGCE LG ++PRNSP DHYCG C
Sbjct: 370 SQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGY 429
Query: 452 GCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSG 511
GC+ G K GT +TWLVD V +G IL G +A R + S +R K F G
Sbjct: 430 GCRAGAKNGTDQTWLVDAVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVG 489
Query: 512 AK--QMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDND 571
K + ++E++VT+ + G+L TPPL+ SGL+NPNIG++L LHPV M WG+FP E D+
Sbjct: 490 GKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS- 549
Query: 572 KAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFK 631
E K YEGGI+T++ ++ D E G A+++ P + P ++ L PWVSG + K
Sbjct: 550 ----EFSGKMYEGGIITSVHHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLK 609
Query: 632 HRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEE 691
RM K+ RTAH+FAL RD GSG V + ++Y+ D ENL+ G+ +ALR+ AAGA E
Sbjct: 610 ERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVE 669
Query: 692 IGTHHNKGRSIKVKKVSYREYEKFVKEESS----RPLNDLATPICSAHQMGSCRMGIGPK 751
+GT+ + G+ +K + ++ E+F+ E + + T SAHQMGSCRMG+ +
Sbjct: 670 VGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAE 729
Query: 752 DSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
+ +++ GE+WE EGLF+ D S+ P+A+GVNPM+T+Q+ AYC + ++D+L+ K
Sbjct: 730 EGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745
BLAST of MC11g0732 vs. NCBI nr
Match:
XP_022142668.1 (long-chain-alcohol oxidase FAO4A [Momordica charantia])
HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 798/798 (100.00%), Postives = 798/798 (100.00%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL
Sbjct: 8 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 67
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR
Sbjct: 68 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 127
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT
Sbjct: 128 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 187
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 240
IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP
Sbjct: 188 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 247
Query: 241 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 300
LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG
Sbjct: 248 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 307
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 360
VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS
Sbjct: 308 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 367
Query: 361 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 420
TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR
Sbjct: 368 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 427
Query: 421 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 480
KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA
Sbjct: 428 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 487
Query: 481 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 540
VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN
Sbjct: 488 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 547
Query: 541 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 600
PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG
Sbjct: 548 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 607
Query: 601 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 660
AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM
Sbjct: 608 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 667
Query: 661 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 720
DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN
Sbjct: 668 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 727
Query: 721 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 780
DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 728 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 787
Query: 781 IAYCTAQSVLDTLKRKRN 798
IAYCTAQSVLDTLKRKRN
Sbjct: 788 IAYCTAQSVLDTLKRKRN 805
BLAST of MC11g0732 vs. NCBI nr
Match:
XP_038896170.1 (long-chain-alcohol oxidase FAO4A [Benincasa hispida])
HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 655/812 (80.67%), Postives = 711/812 (87.56%), Query Frame = 0
Query: 2 AASSGG----DGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNS-LTAAEMES 61
AAS GG DG Q + C++ELHGG D+ +G+ V QHA +S L+AAEMES
Sbjct: 18 AASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSHLSAAEMES 77
Query: 62 LAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKK 121
LAALCDTFLPSVAA ++A + FF+TSASAAGTPERVG MI ERVRHPKK
Sbjct: 78 LAALCDTFLPSVAAT--------AAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKK 137
Query: 122 WMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRM 181
W+VR++LWMLSTWYGTYILCGN SLSNHFPYF+ FSK+S KRE+IA+SWSLS+F L RM
Sbjct: 138 WLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRM 197
Query: 182 FFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENG-RSRE- 241
FFRTIKIL LLAFF+QVDE+ NNISWKAIDYPG DPQFK +T+ SEASNL E G +SRE
Sbjct: 198 FFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREI 257
Query: 242 ------EELYGPLFRGIVNID--QRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDA 301
EELYGPLF+GIVN+ Q+ VA ALRKFGFPV L TK +PSM+IKCDA
Sbjct: 258 DQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDA 317
Query: 302 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNM 361
VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY +SGL+ATDNM
Sbjct: 318 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 377
Query: 362 NVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQ 421
NVL+LAGSTVGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEAMEVVCKKMGVQ
Sbjct: 378 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 437
Query: 422 SEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDL 481
SEI++EGFNNAVLRKGCEELG HA+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDL
Sbjct: 438 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 497
Query: 482 VRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGA 541
VRSGNGAILPGCEA++I EK+DGS+ RAKGI FEFGC S AKQMCLVESKVTIVACGA
Sbjct: 498 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 557
Query: 542 LSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMC 601
L+TPPLLKRSGLRNPNIGK+LHLHPVTMAWG+FPAETD K+WPE+ KKSYEGGIMTAMC
Sbjct: 558 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD--KSWPEKDKKSYEGGIMTAMC 617
Query: 602 NIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKG 661
NIG+E D EPGYGGAVIQTPALHPGLFSILMPWVSGTN KHRMCKFSRTAHVFALARDKG
Sbjct: 618 NIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKG 677
Query: 662 SGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYRE 721
SGTV+SSSS+SYKMD DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYRE
Sbjct: 678 SGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 737
Query: 722 YEKFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVF 781
YEKFVKEESSRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVF
Sbjct: 738 YEKFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVF 797
Query: 782 PTALGVNPMVTVQAIAYCTAQSVLDTLKRKRN 798
PTALGVNPMVTVQAIAYCTAQS+L+TLKRKRN
Sbjct: 798 PTALGVNPMVTVQAIAYCTAQSILETLKRKRN 819
BLAST of MC11g0732 vs. NCBI nr
Match:
XP_022937010.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata])
HSP 1 Score: 1279 bits (3310), Expect = 0.0
Identity = 647/803 (80.57%), Postives = 704/803 (87.67%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AAAS GGDG+ + C++EL+GGAD++ +G P QHA + N SL+AAEMESLAAL
Sbjct: 17 AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF--NYSLSAAEMESLAAL 76
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAA S F F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77 CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
+SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
IKIL LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE NL E RSRE E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEE 256
Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
ELYGPLF+GIVN++ ++ VA ALRKFGFPV L P K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316
Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376
Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFN 436
Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
NAVLRKGCEELG AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496
Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
PGCEA+RI EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TP LLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKR 556
Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
SGLRNPNIGK+LHLHPVTMAWG+FPAETD ++WPE+ KKSYEGGIMTAMCNIG+ D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616
Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676
Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
+SY MD DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736
Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796
Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
VTVQAIAYCTA+SVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAKSVLETLKRKRN 802
BLAST of MC11g0732 vs. NCBI nr
Match:
XP_023535226.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 645/803 (80.32%), Postives = 703/803 (87.55%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AAAS GG+G+ + C+++LHGGAD +G P QHA + N SL+AAEMESLAAL
Sbjct: 17 AAASYGGNGESKPQLHQCVIDLHGGADN--DGGPAGISQHA--NSNYSLSAAEMESLAAL 76
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAA S + F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77 CDTFLPSVAATDGSASEY-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
+SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S +RE++ +SWSLS+FLL RMF+RT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQREREQVVVSWSLSYFLLLRMFYRT 196
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
+KIL LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE NL E RSRE E
Sbjct: 197 VKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256
Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
ELYGPLF+GIVN++ ++ VA ALRKFGFPV L P TK+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGG 316
Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376
Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEIE+EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIENEGFN 436
Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
NAVLRKGCEELG A+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496
Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
PGCEA+RI EK DG ER RAKG+ FEF C SGAKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRERNRAKGVAFEFECGSGAKQMCLVESKVTIVACGALCTPPLLKR 556
Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
SGLRNPNIGK+LHLHPVTMAWG+FPAETD ++WPE+ KKSYEGGIMTAMCNIG+ D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616
Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676
Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
+SY MD DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736
Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796
Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 800
BLAST of MC11g0732 vs. NCBI nr
Match:
KAG6591858.1 (Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1268 bits (3280), Expect = 0.0
Identity = 640/803 (79.70%), Postives = 700/803 (87.17%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
A AS GGDG+ + C++EL+GGAD+ +G P QHA + N SL+AAEMESLAAL
Sbjct: 17 ATASYGGDGESKPQLHQCVIELNGGADSRYDGGPAGISQHA--NSNYSLSAAEMESLAAL 76
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAA S F F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77 CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
+SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S KRE++ +SWSLS+FLL +MFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLKMFFRT 196
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
IKIL LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE NL E RSRE E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256
Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
ELYGPLF+GIVN++ ++ VA ALRKFGFPV L P K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316
Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376
Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
VGGGSTVNWSAS QTP+HVTREWS YEL+LF SNLYKEA+EVVCK+MGVQSE+++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSGEYELELFGSNLYKEALEVVCKRMGVQSEMDNEGFN 436
Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
NA+LRKGCEELG A+NIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAILRKGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496
Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
PGCEA+RI EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPPLLKR 556
Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
SGL NPNIGK+LHLHPV MAWG+FPAETD ++WPE+ KKSYEGGIMTAMCNIG+ D E
Sbjct: 557 SGLTNPNIGKNLHLHPVAMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616
Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
PGYGGAVIQTPALHPGLFSILMPWVSGTN K +MCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQKMCKFSRTAHVFALARDKGSGTVESSSS 676
Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
+SY MD DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736
Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796
Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 802
BLAST of MC11g0732 vs. ExPASy TrEMBL
Match:
A0A6J1CNU3 (Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 SV=1)
HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 798/798 (100.00%), Postives = 798/798 (100.00%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL
Sbjct: 8 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 67
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR
Sbjct: 68 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 127
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT
Sbjct: 128 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 187
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 240
IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP
Sbjct: 188 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGP 247
Query: 241 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 300
LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG
Sbjct: 248 LFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 307
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 360
VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS
Sbjct: 308 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 367
Query: 361 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 420
TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR
Sbjct: 368 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 427
Query: 421 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 480
KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA
Sbjct: 428 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 487
Query: 481 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 540
VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN
Sbjct: 488 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 547
Query: 541 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 600
PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG
Sbjct: 548 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 607
Query: 601 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 660
AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM
Sbjct: 608 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 667
Query: 661 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 720
DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN
Sbjct: 668 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 727
Query: 721 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 780
DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 728 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 787
Query: 781 IAYCTAQSVLDTLKRKRN 798
IAYCTAQSVLDTLKRKRN
Sbjct: 788 IAYCTAQSVLDTLKRKRN 805
BLAST of MC11g0732 vs. ExPASy TrEMBL
Match:
A0A6J1FFC0 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV=1)
HSP 1 Score: 1279 bits (3310), Expect = 0.0
Identity = 647/803 (80.57%), Postives = 704/803 (87.67%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AAAS GGDG+ + C++EL+GGAD++ +G P QHA + N SL+AAEMESLAAL
Sbjct: 17 AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF--NYSLSAAEMESLAAL 76
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
CDTFLPSVAA S F F+TSASAAGTPERVGGMI ERVRHPKKW++R
Sbjct: 77 CDTFLPSVAATDGSASEF-----------FETSASAAGTPERVGGMIKERVRHPKKWLMR 136
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
+SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 VSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
IKIL LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE NL E RSRE E
Sbjct: 197 IKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEE 256
Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
ELYGPLF+GIVN++ ++ VA ALRKFGFPV L P K+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQQ-VAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGG 316
Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
GVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376
Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFN 436
Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
NAVLRKGCEELG AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAVLRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496
Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
PGCEA+RI EK DG +R RAKG+ FEFGC SGAKQMCLVESKVTIVACGAL TP LLKR
Sbjct: 497 PGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKR 556
Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
SGLRNPNIGK+LHLHPVTMAWG+FPAETD ++WPE+ KKSYEGGIMTAMCNIG+ D E
Sbjct: 557 SGLRNPNIGKNLHLHPVTMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616
Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676
Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
+SY MD DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736
Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLFIADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPM 796
Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
VTVQAIAYCTA+SVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAKSVLETLKRKRN 802
BLAST of MC11g0732 vs. ExPASy TrEMBL
Match:
A0A6J1IPF0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1)
HSP 1 Score: 1267 bits (3278), Expect = 0.0
Identity = 642/803 (79.95%), Postives = 700/803 (87.17%), Query Frame = 0
Query: 1 AAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAAL 60
AA+S GGDG+ + C++EL+G AD++ +G P QHA + N SL+AAEMESLAAL
Sbjct: 17 AASSYGGDGESKPQLHQCVIELNGCADSLHDGGPAGISQHA--NSNYSLSAAEMESLAAL 76
Query: 61 CDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVR 120
C+TFLPSVAA S F F+TSASAAGT ERVGGMI ERVRHPKKW++R
Sbjct: 77 CETFLPSVAATDGSASEF-----------FETSASAAGTSERVGGMIKERVRHPKKWLMR 136
Query: 121 LSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRT 180
+SLWMLSTWYGTYILCGN SLSNHFPYFQ FSK+S KRE++ +SWSLS+FLL RMFFRT
Sbjct: 137 MSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRT 196
Query: 181 IKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSRE-----E 240
IKILS LAFF+QVDE+MNNISWKAIDYPG DPQFK + RQSE NL E RSRE E
Sbjct: 197 IKILSHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEE 256
Query: 241 ELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGG 300
ELYGPLF+GIVN++ + VA ALRKFGFPV L P TK+ SPSM+IKCDAVVVGSGSGG
Sbjct: 257 ELYGPLFKGIVNLEHQV-VAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGG 316
Query: 301 GVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGST 360
GVV GVLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVL+LAGST
Sbjct: 317 GVVGGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGST 376
Query: 361 VGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFN 420
VGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEA+EVVCK+MGVQSEI++EGFN
Sbjct: 377 VGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIDNEGFN 436
Query: 421 NAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 480
NA+LRKGCEELG AINIPRNS +DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL
Sbjct: 437 NAILRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAIL 496
Query: 481 PGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKR 540
PGCEA+RI EK DG ER RAKG+ FEF C S AKQMCLVESKVTIVACGAL TPPLLKR
Sbjct: 497 PGCEAIRITQEK-DGRERNRAKGVAFEFECGSEAKQMCLVESKVTIVACGALCTPPLLKR 556
Query: 541 SGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNE 600
SGLRNPNIGK+LHLHPV MAWG+FPAETD ++WPE+ KKSYEGGIMTAMCNIG+ D E
Sbjct: 557 SGLRNPNIGKNLHLHPVAMAWGYFPAETD--ESWPEKDKKSYEGGIMTAMCNIGSSPDQE 616
Query: 601 PGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSS 660
PGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVFALARDKGSGTV SSSS
Sbjct: 617 PGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSS 676
Query: 661 ISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEES 720
+SY MD DE+NLQKG+EKAL+IL AAGAEEIGTHHNKGRSI VKKVSYREYE+FVKEES
Sbjct: 677 LSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEES 736
Query: 721 SRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPM 780
SRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGLF+ADSSVFPTALGVNPM
Sbjct: 737 SRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFVADSSVFPTALGVNPM 796
Query: 781 VTVQAIAYCTAQSVLDTLKRKRN 798
VTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 VTVQAIAYCTAQSVLETLKRKRN 802
BLAST of MC11g0732 vs. ExPASy TrEMBL
Match:
A0A1S3BAP1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1)
HSP 1 Score: 1266 bits (3275), Expect = 0.0
Identity = 644/815 (79.02%), Postives = 704/815 (86.38%), Query Frame = 0
Query: 1 AAASSGGDGDGE---QHRRHCIVELHGGADTILNGSPVQNPQHAK-----YSHNNSLTAA 60
AAA+ GG DGE Q HC++ELHGGAD+ +G+ V+ QHA YS L+AA
Sbjct: 17 AAANGGGGDDGETNHQPHHHCVIELHGGADSFHDGASVEISQHANSNNDYYSSAGHLSAA 76
Query: 61 EMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVR 120
EMESLAALCDTFLPSVA AT+ + TA FF+TSASA GTPERVG MI ERVR
Sbjct: 77 EMESLAALCDTFLPSVAPATAADRS--------TAVFFETSASAVGTPERVGEMIMERVR 136
Query: 121 HPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFL 180
HPKKW+VR+SLWMLSTWYGTYILCGN SLSNHFPYF+ FSK+S KRE+IALSWSLS+F
Sbjct: 137 HPKKWLVRVSLWMLSTWYGTYILCGNESLSNHFPYFKRFSKVSQKKREQIALSWSLSYFF 196
Query: 181 LHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFK----LETRQSEASNLNE 240
L RMFFRTIKIL LLAFF+QVDE+MNNISWKAIDYPG DPQFK L+ + S++ ++E
Sbjct: 197 LLRMFFRTIKILCLLAFFTQVDEEMNNISWKAIDYPGSDPQFKPPSNLKEKDSKSREVDE 256
Query: 241 NGRSREEE---LYGPLFRGIVNID--QRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIK 300
G EEE LYGPLF+GIVN+ Q+ VA ALRKFGFPV L P TK +PSM+IK
Sbjct: 257 EGDEEEEEAEELYGPLFKGIVNLQHQQQIQVAVGALRKFGFPVTLLSPTTKPPNPSMIIK 316
Query: 301 CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLAT 360
CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY SSGL+AT
Sbjct: 317 CDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQSSGLVAT 376
Query: 361 DNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKM 420
DNMNVL+LAGSTVGGGSTVNWSAS +TP+HVT+EWSD YEL+LF SNLYKEA++VVCKKM
Sbjct: 377 DNMNVLILAGSTVGGGSTVNWSASIKTPSHVTKEWSDEYELELFRSNLYKEAIDVVCKKM 436
Query: 421 GVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWL 480
GVQS+I++EGFNNAVLRKGCEELG HAINIPRNS +DHYCGWCCLGC++GRKKGTAETWL
Sbjct: 437 GVQSKIDNEGFNNAVLRKGCEELGGHAINIPRNSTSDHYCGWCCLGCRDGRKKGTAETWL 496
Query: 481 VDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVA 540
VDLVRSGNG ILPGCEA++I EKK G ERK AKG+ FEF C S AKQMCLVESKVTIVA
Sbjct: 497 VDLVRSGNGVILPGCEAIKIRQEKKQGRERKMAKGVAFEFWCGSAAKQMCLVESKVTIVA 556
Query: 541 CGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMT 600
CGAL+TPPLLKRSGLRNPNIGK+LHLHPVTMAWG+FPAETD K+WPE+ KKSYEGGIMT
Sbjct: 557 CGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD--KSWPEKDKKSYEGGIMT 616
Query: 601 AMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALAR 660
AMCNIG E + EPGYGGAVIQTPALHPGLFSILMPWVSGTN K RMCKFSRTAHVF LAR
Sbjct: 617 AMCNIGPEPNQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFVLAR 676
Query: 661 DKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVS 720
DKGSGTV+S SS+SYKMD DE+NLQKG+EKAL+ILAAAGA EIGTHHNKGRSI VKKVS
Sbjct: 677 DKGSGTVKSPSSLSYKMDNVDEQNLQKGLEKALKILAAAGAVEIGTHHNKGRSINVKKVS 736
Query: 721 YREYEKFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADS 780
YREYEKFVKEESSRPL DL TPICSAHQMGSCRMG G KDSVVNQRGETWE+EGLFIADS
Sbjct: 737 YREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGSGAKDSVVNQRGETWEVEGLFIADS 796
Query: 781 SVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRKRN 798
SVFPTALGVNPMVTVQAIAYCTAQSVL+TLKRKRN
Sbjct: 797 SVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKRN 821
BLAST of MC11g0732 vs. ExPASy TrEMBL
Match:
A0A6J1FQT3 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV=1)
HSP 1 Score: 1243 bits (3216), Expect = 0.0
Identity = 635/798 (79.57%), Postives = 689/798 (86.34%), Query Frame = 0
Query: 6 GGDGDGEQHRR--HCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAALCDT 65
GG GD E R+ H ++ELH GA P + +HA ++N SL+AAEMESLAALCDT
Sbjct: 54 GGGGDDEAKRKLDHYVIELHDGA-------PAEILEHANSNNNCSLSAAEMESLAALCDT 113
Query: 66 FLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRHPKKWMVRLSL 125
FLPSV A S A FF+TSA+AAGTPE+VG MI ERV+HPKKW+VR+SL
Sbjct: 114 FLPSVTADGSA------------AMFFETSAAAAGTPEKVGKMIKERVKHPKKWLVRVSL 173
Query: 126 WMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLLHRMFFRTIKI 185
WMLSTWYGTYILCGN SLS++FPYFQTF KLS KRE+I +SWSLS+F L RM+FRTIKI
Sbjct: 174 WMLSTWYGTYILCGNRSLSHNFPYFQTFPKLSQTKREQIVVSWSLSYFFLLRMWFRTIKI 233
Query: 186 LSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGPLFR 245
LL FF+QVD+K NNISW+AIDYPG DPQFK +T+Q EAS E EEELYGPLF+
Sbjct: 234 FCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEASREVEED---EEELYGPLFK 293
Query: 246 GIVNIDQRR---GVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAG 305
GIV++D ++ AAEALRKFGFPV L P K +SPSM+IKCDAVVVGSGSGGGVVAG
Sbjct: 294 GIVHLDNQQVAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKCDAVVVGSGSGGGVVAG 353
Query: 306 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGS 365
VLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGL+ATDNMNVLVLAGSTVGGGS
Sbjct: 354 VLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 413
Query: 366 TVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLR 425
TVNWSAS QTP+HVTREWSD YEL+LFSSNLYKEAMEVVCKKMGVQSEI++EGFNNAVLR
Sbjct: 414 TVNWSASIQTPSHVTREWSDKYELELFSSNLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 473
Query: 426 KGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 485
KGCEELGYHAINIPRNS +DHYCGWCCLGCK+GRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 474 KGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLVDLVRSGNGVILPGCEA 533
Query: 486 VRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRN 545
+ I EKKDGSER RAKGI FEFGC SG KQ+CLVESKVTIVACGALSTPPLL RSGLRN
Sbjct: 534 IDIRKEKKDGSERNRAKGIAFEFGCGSGVKQVCLVESKVTIVACGALSTPPLLLRSGLRN 593
Query: 546 PNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGG 605
PNIGK+LHLHPVTMAWGHFPAETD K WPEE KKSYEGGIMTAMCNI +E D EPG GG
Sbjct: 594 PNIGKNLHLHPVTMAWGHFPAETD--KPWPEEHKKSYEGGIMTAMCNIRSEPDQEPGSGG 653
Query: 606 AVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKM 665
AVIQTPALHPGLFSILMPW+SGTN K RM KFSRTAHVF LARDKGSGTV+S + ISY M
Sbjct: 654 AVIQTPALHPGLFSILMPWLSGTNIKQRMRKFSRTAHVFVLARDKGSGTVKSPNCISYNM 713
Query: 666 DGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRPLN 725
+ DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYREYEKFVKEESSR L
Sbjct: 714 EEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRALK 773
Query: 726 DLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQA 785
DL TPICSAHQMGSCRMGIG +DSVV+QRGETWE+EGLFIADSSVFPTALGVNPMVTVQA
Sbjct: 774 DLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVFPTALGVNPMVTVQA 827
Query: 786 IAYCTAQSVLDTLKRKRN 798
IAYCTAQSVL+TLKRKRN
Sbjct: 834 IAYCTAQSVLETLKRKRN 827
BLAST of MC11g0732 vs. TAIR 10
Match:
AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 863.2 bits (2229), Expect = 1.6e-250
Identity = 436/747 (58.37%), Postives = 556/747 (74.43%), Query Frame = 0
Query: 54 MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 113
MESL A+CDTF+ S+ + GH + A +F SAS GTP+RV ++SER+ H
Sbjct: 1 MESLVAICDTFISSIDDSGVGHVD------DCVAGYFSASASQTGTPDRVARLMSERLHH 60
Query: 114 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 173
PKKW++R LW+LSTW G+ +LCG S + FPYF+ F +L +RE+I L+WS S+F L
Sbjct: 61 PKKWILRAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSL 120
Query: 174 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 233
RM FRTIK++S L FF+QVDEK N++WKAI Y GP P + E E + +
Sbjct: 121 LRMLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSP----DHSDHEVELNEEKKKKK 180
Query: 234 EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTAS---PSMMIKCDAVVVG 293
EE++GPL+ GIV++ R + L GF V+ T +S P M I+CDAVVVG
Sbjct: 181 PEEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVG 240
Query: 294 SGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNVLV 353
SGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GLLAT + NV++
Sbjct: 241 SGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVI 300
Query: 354 LAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSEIE 413
LAGSTVGGGST+NWSAS +TP HV +EW++ +L++F S+LY+EAM+VVCK+MGVQ
Sbjct: 301 LAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFV 360
Query: 414 SEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVRSG 473
EGFNN VLRKGCE+LG NIPRN+P+DHYCG+CCLGCK G+K+GT+ETWLVDLV S
Sbjct: 361 EEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESD 420
Query: 474 NGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTP 533
NG ILPGC+A +M++ + G ++K+A G+ F FG +++ +VES+VTIVACGAL TP
Sbjct: 421 NGLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTP 480
Query: 534 PLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGT 593
LLKRSGL+N NIG++L LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++
Sbjct: 481 HLLKRSGLKNSNIGRNLCLHPVVMAWGWFPEE---DK-WPEKKKKSYEGGIMTAMSSVVI 540
Query: 594 EADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTV 653
E + YG VIQTPALHPG+FS ++PW S +FK RM KFSRTAH+FAL RDKG+GT+
Sbjct: 541 E-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTI 600
Query: 654 RSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKF 713
S + I Y ++ DEE+L+ G+E+ L+ILAAAGAEEIGTHH++GRS+ V+ S E E+F
Sbjct: 601 DSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERF 660
Query: 714 VKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTAL 773
V+EESS+PL DL+ ICSAHQMGSCRMGI P++S V GETWE+E LF+AD+SVFPTAL
Sbjct: 661 VREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTAL 720
Query: 774 GVNPMVTVQAIAYCTAQSVLDTLKRKR 798
GVNPMVTVQ+IAYC +V+D LK+K+
Sbjct: 721 GVNPMVTVQSIAYCIGLNVVDVLKKKK 726
BLAST of MC11g0732 vs. TAIR 10
Match:
AT3G23410.1 (fatty alcohol oxidase 3 )
HSP 1 Score: 525.4 bits (1352), Expect = 8.0e-149
Identity = 313/763 (41.02%), Postives = 449/763 (58.85%), Query Frame = 0
Query: 49 LTAAEMESLAALCDTFLPSV-------AAATSGHSNFESSAATATAAFFQTSASAAGTPE 108
+T AEMESLA+ C+ LPSV + H N E A +F+ TS S
Sbjct: 15 ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKE-----ALRSFYSTSGSKTPVLR 74
Query: 109 RVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREK 168
+ ++++R RL L++L+T GT ++CG L + +P+ + FS+LS KRE+
Sbjct: 75 QSIELVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRER 134
Query: 169 IALSWSLSFFLLH-RMFFRTIKILSLLAFFSQVDEKMNNISWKAIDY-PGPDPQFKLETR 228
+ ++ L R F IK+ L FFS+V+ N +W+AI Y PD E +
Sbjct: 135 VLQKQFKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPD-----ENK 194
Query: 229 QSEASNLNENGRSREEELYGPLFRGIV-NIDQRRGVAAEALRKFGFPVAFLPPRTKTASP 288
SE N PL +GIV +++ E+L G T
Sbjct: 195 PSETHNER------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHD 254
Query: 289 SMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 348
++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY + EG ++++Y +
Sbjct: 255 AIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENG 314
Query: 349 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 408
G+L + + + +VLAG+TVGGGS VNWSA +TP V +EWS+ + LF + Y AMEV
Sbjct: 315 GILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEV 374
Query: 409 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 468
V K+MGV + E E F N +LRKGCE LG++ N+PRNS HYCG C GC+ G KKG+
Sbjct: 375 VWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGS 434
Query: 469 AETWLVDLVRSGNGA-ILPGCEAVRIMWEK---KDGSERKRAKGITFEFGCQSGAKQMCL 528
TWLVD V G+GA IL GC+A R + EK G ++ + G+ + +M
Sbjct: 435 DRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAK-SLNGNIAKMLK 494
Query: 529 VESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGK 588
+E+KVT+ A GAL TPPL+ SGLRN NIGK+LHLHPV MAWG+FP + ++ ++
Sbjct: 495 IEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFK---G 554
Query: 589 KSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSR 648
SYEGGI+T++ + +E D+E A+I+TP L PG FS+L PW SG + K RM ++SR
Sbjct: 555 NSYEGGIITSVSKVLSE-DSEV---RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSR 614
Query: 649 TAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKG 708
TA + + RD+GSG V++ I+Y +D D +NL+ G+ ++LRIL AAGAEE+GTH + G
Sbjct: 615 TASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDG 674
Query: 709 RSIKVKKVSYREYEKFV----KEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRG 768
+ + K V+ ++F+ EE ++ + + SAHQMGSCR+G K+ ++ G
Sbjct: 675 QRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNG 734
Query: 769 ETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTL 794
E+WE E LF+ D+S P+A+GVNPM+TV + AYC + + ++
Sbjct: 735 ESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740
BLAST of MC11g0732 vs. TAIR 10
Match:
AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 520.0 bits (1338), Expect = 3.4e-147
Identity = 306/774 (39.53%), Postives = 442/774 (57.11%), Query Frame = 0
Query: 32 GSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQ 91
G P+ + +N+ + ++++SLA +C TFLP +S A +F
Sbjct: 10 GHPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPPET----------TSEQQAVNSFHV 69
Query: 92 TSASAAGTPERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTF 151
S++ + V MI + R ++R+ L +LS +GT +LCG+ L +P+ F
Sbjct: 70 ASSTQPPFTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKF 129
Query: 152 SKLSHNKREKIALSWS--LSFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPG 211
S+L +KRE I +WS F L R+ F K +L FFSQ DE + N + +AI Y
Sbjct: 130 SQLPLDKREAILRNWSRQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-- 189
Query: 212 PDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVA-AEALRKFGFPVAF 271
+ + + N +S +E PL +GI+ V ++L + G VA
Sbjct: 190 ----------CIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA- 249
Query: 272 LPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG 331
I+CDAVVVGSGSGGGV A LA AG KVLVLEKG+Y + S LE
Sbjct: 250 ----RDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEV 309
Query: 332 QAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFS 391
+M E+Y GLL T + ++LAGS VGGG+ VNWSAS +TP HV +EWS+ ++ F
Sbjct: 310 PSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFG 369
Query: 392 SNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCL 451
S Y+ AM+ V ++GV GF N VLRKGCE LG ++PRNSP DHYCG C
Sbjct: 370 SQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGY 429
Query: 452 GCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSG 511
GC+ G K GT +TWLVD V +G IL G +A R + S +R K F G
Sbjct: 430 GCRAGAKNGTDQTWLVDAVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVG 489
Query: 512 AK--QMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDND 571
K + ++E++VT+ + G+L TPPL+ SGL+NPNIG++L LHPV M WG+FP E D+
Sbjct: 490 GKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS- 549
Query: 572 KAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFK 631
E K YEGGI+T++ ++ D E G A+++ P + P ++ L PWVSG + K
Sbjct: 550 ----EFSGKMYEGGIITSVHHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLK 609
Query: 632 HRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEE 691
RM K+ RTAH+FAL RD GSG V + ++Y+ D ENL+ G+ +ALR+ AAGA E
Sbjct: 610 ERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVE 669
Query: 692 IGTHHNKGRSIKVKKVSYREYEKFVKEESS----RPLNDLATPICSAHQMGSCRMGIGPK 751
+GT+ + G+ +K + ++ E+F+ E + + T SAHQMGSCRMG+ +
Sbjct: 670 VGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAE 729
Query: 752 DSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRK 797
+ +++ GE+WE EGLF+ D S+ P+A+GVNPM+T+Q+ AYC + ++D+L+ K
Sbjct: 730 EGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745
BLAST of MC11g0732 vs. TAIR 10
Match:
AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-140
Identity = 291/788 (36.93%), Postives = 452/788 (57.36%), Query Frame = 0
Query: 24 GGADTILNGSPVQNPQHAKYSHNNSLTAAEMESLAALCDTFLPSVAAATSG-HSNFESSA 83
GG GSP+ + S ++ + +++++L+++CD +P V + +
Sbjct: 3 GGRRLGKRGSPLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLR 62
Query: 84 ATATAAFFQTSASAAGT-PERVGGMISERVRHPKKWMVRLSLWMLSTWYGTYILCGNASL 143
A +FF++S+S + P+ V +++ + +VR+ L +L+ GT +LCG L
Sbjct: 63 NDALLSFFKSSSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCL 122
Query: 144 -SNHFPYFQTFSKLSHNKREKIALSWSLSFF-LLHRMFFRTIKILSLLAFFSQVDEKMNN 203
H+P+ FS++S KREK+ W+ ++ L R+ F IK + L +F+ +E N
Sbjct: 123 DKKHWPFLLKFSEMSLEKREKVLQRWNTQWYNPLARIGFMMIKAIFLFYYFTWTNENSEN 182
Query: 204 ISWKAIDYPGPDPQFKLETRQSEASNLNENGRSREEELYGPLFRGIVNIDQRRGVAAEAL 263
W AI+Y + + EN ++E PL GI+ + + +
Sbjct: 183 PVWDAINY---------------SVEIGENEDMEQKE--RPLDEGIIETAKEDEMTIKQ- 242
Query: 264 RKFGFPVAFLPPRTKTASPSMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCA 323
R + R + + I+CDAVVVGSG GGGV A +LA +G +V+V+EKG+Y A
Sbjct: 243 RMINKGLKVTEDRER---DTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFA 302
Query: 324 RTNLSLLEGQAMEEMYLSSGLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWS 383
+ S LEG +M E++ S+ L+ T + +AGSTVGGGS VNW+AS +TP + EWS
Sbjct: 303 PRDYSALEGPSMFELFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWS 362
Query: 384 DVYELDLFSSNLYKEAMEVVCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPA 443
+ ++SS YK AM +VCK++GV +I EGF N +LRKGCE+LG +PRNS
Sbjct: 363 VHRGISIYSSEKYKAAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTE 422
Query: 444 DHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEAVRIMW------EKKDGSER 503
HYCG C GC G K+GT TWLVD V + N IL C+A +++ ++++ R
Sbjct: 423 KHYCGSCSYGCPTGEKRGTDSTWLVDAV-NNNAVILTQCKAEKLILADNDANKREESGRR 482
Query: 504 KRAKGITFEFGCQSGAKQMCLVESKVTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVT 563
KR G+ Q+ K + +KVTIVACG+L TP LL SGL+N NI + LH+HP+
Sbjct: 483 KRCLGVAASLSHQTRKKLQ--INAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHPIM 542
Query: 564 MAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLF 623
MAWG+FP + E ++EG I+T++ + P ++TPA+ PG F
Sbjct: 543 MAWGYFPEKNS------ELEGAAHEGEIVTSLHYVHPMDSTTP---NITLETPAIGPGTF 602
Query: 624 SILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSGTVRSSSSISYKMDGADEENLQKGVE 683
+ L PWVSG++ K RM K++RTAH+FA+ RD+G G V+ + Y++ ADEENL G++
Sbjct: 603 AALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVK-GDIVKYRLTKADEENLTIGLK 662
Query: 684 KALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYEKFVKEESSRP----LNDLATPICSA 743
+ALRIL AAGA E+GT+ + G+ +K + ++ E F+ ++ P ++ T +A
Sbjct: 663 QALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTA 722
Query: 744 HQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSV 798
HQ+G CRMG K+ ++ +GE+WE E L++ D+SV PTALGVNPM+TVQ+ AYC + +
Sbjct: 723 HQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRI 756
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O65709 | 2.3e-249 | 58.37 | Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 S... | [more] |
| B5WWZ8 | 1.7e-159 | 41.85 | Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1 | [more] |
| B5WWZ9 | 9.8e-152 | 41.15 | Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1 | [more] |
| Q9LW56 | 1.1e-147 | 41.02 | Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... | [more] |
| Q94BP3 | 4.7e-146 | 39.53 | Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022142668.1 | 0.0 | 100.00 | long-chain-alcohol oxidase FAO4A [Momordica charantia] | [more] |
| XP_038896170.1 | 0.0 | 80.67 | long-chain-alcohol oxidase FAO4A [Benincasa hispida] | [more] |
| XP_022937010.1 | 0.0 | 80.57 | long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata] | [more] |
| XP_023535226.1 | 0.0 | 80.32 | long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG6591858.1 | 0.0 | 79.70 | Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CNU3 | 0.0 | 100.00 | Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 S... | [more] |
| A0A6J1FFC0 | 0.0 | 80.57 | Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV... | [more] |
| A0A6J1IPF0 | 0.0 | 79.95 | Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1 | [more] |
| A0A1S3BAP1 | 0.0 | 79.02 | Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1 | [more] |
| A0A6J1FQT3 | 0.0 | 79.57 | Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G19380.1 | 1.6e-250 | 58.37 | Long-chain fatty alcohol dehydrogenase family protein | [more] |
| AT3G23410.1 | 8.0e-149 | 41.02 | fatty alcohol oxidase 3 | [more] |
| AT4G28570.1 | 3.4e-147 | 39.53 | Long-chain fatty alcohol dehydrogenase family protein | [more] |
| AT1G03990.1 | 4.0e-140 | 36.93 | Long-chain fatty alcohol dehydrogenase family protein | [more] |
