MC11g0673 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0673 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 7.9e-236
Identity = 406/576 (70.49%), Postives = 470/576 (81.60%), Query Frame = 0

Query: 9   SHAT---SSVSRSSIGDNS-------SYLCSTASNSSYPLQFHEQKDEKFCFVN------ 68
           SHA+   +   R+ I D+S       S+     + S  PLQ  EQK EK   V+      
Sbjct: 17  SHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDG 76

Query: 69  GDADGHSEDEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQ 128
           G      EDE F +LG  + +KRQRD    L Q+   + +   +S LE+RRA+VRAWG+Q
Sbjct: 77  GGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQ 136

Query: 129 SLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT 188
            + +ADP+IHE+M KE+QRQ +GIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT
Sbjct: 137 PIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT 196

Query: 189 GNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSA 248
           GNQ ID+IE LC ERAL AF L+S+KWGVNVQPYSCTSANFAVYTGLLLP +R+MGLDS 
Sbjct: 197 GNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSP 256

Query: 249 SGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGS 308
           SGGH+SHGY TPGGKKISA+SIFFES PYKVNPQTGY+DYDKLE+KALDYRPKILICGGS
Sbjct: 257 SGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGS 316

Query: 309 SYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPR 368
           SYPR+WD+AR+RQIADKCGAVLMCDMAHISGLVA KEC++PF++CDIVTSTTHK LRGPR
Sbjct: 317 SYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPR 376

Query: 369 GGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQ 428
           GGIIF+RRG K RKQG   +H D +  YD EE+INF+VFPSLQGGPHNNHIAALAIALKQ
Sbjct: 377 GGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQ 436

Query: 429 VASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICE 488
           VA+ EY  YI+Q+KKNAQALA+ALLRRKCRLVT GTDNHL+LWDLTP+GLT K YEK+CE
Sbjct: 437 VATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCE 496

Query: 489 LCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHR 548
           +CHIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFETMA+ L+KAAQIT   + R
Sbjct: 497 MCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITS-ALQR 556

Query: 549 EHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMP 569
           EHG+ HK+ + +L  NKDI ELRNRVEAFA  + MP
Sbjct: 557 EHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of MC11g0673 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.6e-228
Identity = 397/593 (66.95%), Postives = 470/593 (79.26%), Query Frame = 0

Query: 2   DLSLRF-SSHA--TSSVSRSSIGD-------NSSYLCSTASNSSYPLQFHEQK------- 61
           DLSL F SSHA       R  I D       +SS+  S+      PLQ  EQ+       
Sbjct: 8   DLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGSC 67

Query: 62  -----DEKFCFVNGDADGHSEDEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSL 121
                ++     + D D   E+E+F LLG P+++KR R G S+   +S+       +S +
Sbjct: 68  SRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGI 127

Query: 122 EARRASVRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTN 181
           E+RRA VRAWGNQS+  ADPEIHE M KE+QRQ++GIELIASENFVCRAVMEALGSHLTN
Sbjct: 128 ESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTN 187

Query: 182 KYSEGMPGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGL 241
           KYSEGMPGARYYTGNQ ID+IE+LC ERALAAF L+ EKWGVNVQPYSCTSANFAV+TGL
Sbjct: 188 KYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGL 247

Query: 242 LLPKDRVMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKA 301
           L+P +R+MGLDS SGGH+SHGYYTPGGKK+S +SIFFES PYKV+P+TGY+DYDKLEEKA
Sbjct: 248 LMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKA 307

Query: 302 LDYRPKILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDI 361
           LDYRPKILICGGSSYPR+W++ R R IADKCGAVLM DMA ISGLVAAKE  +PF+YCDI
Sbjct: 308 LDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDI 367

Query: 362 VTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPH 421
           VTSTTHKSLRGPRGGIIF++RGLK +KQ + L H +    YDFEE+INFSVFPSLQGGPH
Sbjct: 368 VTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPH 427

Query: 422 NNHIAALAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTP 481
           NNHIAALAIALKQ AS EY +Y+RQVKKNA+ALASAL+ RKC+L+T GTDNHL+LWDLTP
Sbjct: 428 NNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTP 487

Query: 482 LGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEIL 541
           LGLT K YEK+CE+CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FETMA+ L
Sbjct: 488 LGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFL 547

Query: 542 LKAAQITKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 573
            +AAQI      REHG+L K+ + ++ + K+I +LRN+VEAFA+ FAMP FD+
Sbjct: 548 YRAAQIAS-AAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of MC11g0673 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 3.5e-175
Identity = 296/465 (63.66%), Postives = 366/465 (78.71%), Query Frame = 0

Query: 106 VRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGM 165
           V +WGN SL   DPEIH+++ KE++RQ +GIELIASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 166 PGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDR 225
           PG RYY GN+ IDEIE LC  RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 226 VMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPK 285
           +MGLD  SGGHL+HGYYT GGKKISA+SI+FESLPYKVN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 286 ILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 345
           +LICGGS+YPR+WDYAR R IADK GA+L+CDMAHISGLVAA+E A+PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 346 KSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAA 405
           KSLRGPR G+IF+R+G K  K+G       +   YDFE++INF+VFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 406 LAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAK 465
           LA+ALKQ  +  + VY +QVK NA AL + L+ +  ++VT+GT+NHLVLWDL PLGLT  
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 466 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQI 525
             EK+C+LC IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE + E L +A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 526 TKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGF 571
           T + I + +G+L KD    L NNKD+ +L+  VE F++S+ MPGF
Sbjct: 424 T-LDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of MC11g0673 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 8.8e-171
Identity = 287/467 (61.46%), Postives = 357/467 (76.45%), Query Frame = 0

Query: 106 VRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGM 165
           V +WGN  L   DPEI++++ KE+ RQ +GIELIA+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 166 PGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDR 225
           PG RYY G + IDEIE LC  R+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 226 VMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPK 285
           +MGLD  SGGH++HGYY+ GGK ISA+SI+FE+LPYKV+ +TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 286 ILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 345
           ++ICGG+SYPREWDYAR R +ADK GA L+CDMAH S LVAA+E A PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 346 KSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAA 405
           KSLRGPR G+IF+R+G K  K+G       + + YDF+ +IN +VFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 406 LAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAK 465
           LA+ALKQV +  + VY +QVK NA  LAS L+ +   LVTDGTDNHL+LWDL PLGLT  
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 466 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQI 525
             EK+CELC+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE + E L +A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 526 TKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 573
           T + I  ++G++ KD    L NNK+I E++  VE F   F MPGF I
Sbjct: 424 T-LDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of MC11g0673 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 7.5e-154
Identity = 269/460 (58.48%), Postives = 340/460 (73.91%), Query Frame = 0

Query: 110 GNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGAR 169
           GN  L+  D EI E+M +E+ RQ+KG+ELIASENF  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 170 YYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGL 229
           YY G + +DE+E LC +RAL AF LD  KWGVNVQPYS + ANFAVYT LL P DR+MGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 230 DSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILIC 289
           D  SGGHL+HGY T   KKISASSIFFES+PY++    G +DY +LEE AL ++PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 290 GGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLR 349
           G S+YPREWDY R+R IADK GA LMCDMAH SGLVAA+   SPF+YCD+VTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 350 GPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIA 409
           GPR GIIFFRRG   R  G    +G + + YD E +INF+VFPSLQGGPH N IA +A+A
Sbjct: 246 GPRSGIIFFRRG--KRVDG----NGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 410 LKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEK 469
           LK+  S E+  Y  QVKKNA A+ +AL+ +  +LVT+GTDNHL+LWDL P  LT   +EK
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 470 ICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIV 529
             ++ +ITVNK A++GD  AISPGG+RIG+ A+T+RG  EADFE +A+ L +   I+ + 
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSIS-LE 425

Query: 530 IHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPG 570
           I    G+   D +  +  +K++++LR  VE F+S F +PG
Sbjct: 426 IQGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of MC11g0673 vs. NCBI nr
Match: XP_022137046.1 (serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0

Query: 1   MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE 60
           MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE
Sbjct: 1   MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE 60

Query: 61  DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE 120
           DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE
Sbjct: 61  DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE 120

Query: 121 IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI 180
           IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI
Sbjct: 121 IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI 180

Query: 181 ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG 240
           ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG
Sbjct: 181 ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG 240

Query: 241 YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 300
           YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY
Sbjct: 241 YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 300

Query: 301 ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR 360
           ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR
Sbjct: 301 ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR 360

Query: 361 GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV 420
           GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV
Sbjct: 361 GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV 420

Query: 421 YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK 480
           YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK
Sbjct: 421 YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK 480

Query: 481 CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD 540
           CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD
Sbjct: 481 CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD 540

Query: 541 IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI
Sbjct: 541 IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572

BLAST of MC11g0673 vs. NCBI nr
Match: KAG7031702.1 (Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1070 bits (2766), Expect = 0.0
Identity = 521/571 (91.24%), Postives = 549/571 (96.15%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLEARRASVRAWGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKECASPF+YCD+VTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HG++T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQVAS EY VY
Sbjct: 369 LKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKDI
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDI 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. NCBI nr
Match: KAG6608069.1 (Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 521/571 (91.24%), Postives = 549/571 (96.15%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDFHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLEARRASVRAWGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKECASPF+YCD+VTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HG++T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQVAS EY VY
Sbjct: 369 LKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKDI
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDI 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. NCBI nr
Match: XP_022980970.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 521/571 (91.24%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLEARRASVRAWGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HGD+T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQVAS EY VY
Sbjct: 369 LKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKDI
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDI 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. NCBI nr
Match: XP_022941000.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata])

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 516/571 (90.37%), Postives = 546/571 (95.62%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           E+FSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLE RRASVRAWGNQSLR+ADPEI
Sbjct: 69  EQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKI+ICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HG++T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQV S EY VY
Sbjct: 369 LKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVTSLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKD+
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDM 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. ExPASy TrEMBL
Match: A0A6J1C598 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008604 PE=3 SV=1)

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0

Query: 1   MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE 60
           MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE
Sbjct: 1   MDLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSE 60

Query: 61  DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE 120
           DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE
Sbjct: 61  DEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPE 120

Query: 121 IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI 180
           IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI
Sbjct: 121 IHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEI 180

Query: 181 ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG 240
           ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG
Sbjct: 181 ELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHG 240

Query: 241 YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 300
           YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY
Sbjct: 241 YYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 300

Query: 301 ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR 360
           ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR
Sbjct: 301 ARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRR 360

Query: 361 GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV 420
           GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV
Sbjct: 361 GLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGV 420

Query: 421 YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK 480
           YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK
Sbjct: 421 YIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNK 480

Query: 481 CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD 540
           CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD
Sbjct: 481 CAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKD 540

Query: 541 IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI
Sbjct: 541 IMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572

BLAST of MC11g0673 vs. ExPASy TrEMBL
Match: A0A6J1IY33 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3 SV=1)

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 521/571 (91.24%), Postives = 547/571 (95.80%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLEARRASVRAWGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HGD+T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQVAS EY VY
Sbjct: 369 LKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKDI
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDI 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. ExPASy TrEMBL
Match: A0A6J1FSB1 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE=3 SV=1)

HSP 1 Score: 1061 bits (2745), Expect = 0.0
Identity = 516/571 (90.37%), Postives = 546/571 (95.62%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLSLRFSSHATSS+SRSSIGDNSSYLCSTASNSSY L+  EQKDEKF FV GD D HS+D
Sbjct: 9   DLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKGDPDDHSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           E+FSLLGQPLRVKRQRD +SFLNQ SAKR+A+ DESSLE RRASVRAWGNQSLR+ADPEI
Sbjct: 69  EQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H+IMAKE+QRQY+GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ+IDEIE
Sbjct: 129 HDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQHIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCCERALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           YTPGGKKISA+SIFF+SLPYKVNP TGYVDYDKLEEKALDYRPKI+ICGGSSYPREWDY 
Sbjct: 249 YTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSSYPREWDYG 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYGVY 421
           LKSRKQGVLL HG++T TYDFE+RINFSVFPSLQGGPHNNHIAALAIALKQV S EY VY
Sbjct: 369 LKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVTSLEYRVY 428

Query: 422 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVNKC 481
           IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDL PLGLTAKNYEKICELCHITVNKC
Sbjct: 429 IRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICELCHITVNKC 488

Query: 482 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHREHGRLHKDI 541
           AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKA QITK+V+HREHG+LHKD+
Sbjct: 489 AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHREHGKLHKDM 548

Query: 542 MNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           M NLQNNKDI+ELRNRVEAFA+SFAMPGFDI
Sbjct: 549 MKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of MC11g0673 vs. ExPASy TrEMBL
Match: A0A5A7VB49 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001500 PE=3 SV=1)

HSP 1 Score: 1017 bits (2630), Expect = 0.0
Identity = 506/574 (88.15%), Postives = 528/574 (91.99%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLS R SSH TSS+SRSS GDNSSYLCST SNSSYP  F +QKDEKF  V G  D  S+D
Sbjct: 9   DLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKGVVDDDSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD  SFL+Q SAKR AV DE+SLE RRASV+ WGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H IM KE++RQYKGIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ IDEIE
Sbjct: 129 HNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCC RALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           Y+P GKKISA+SIFFESLPY+VNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA
Sbjct: 249 YSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDT--DTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYG 421
           LKSRKQGVLLTHGDD    TYDFE+RINFSVFPSLQGGPHNNH+AALAIALKQVAS EY 
Sbjct: 369 LKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALKQVASPEYR 428

Query: 422 VYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVN 481
           VYIRQVKKNAQALASALLRR CRLVT+GTDNHL+LWDLTPLGLTAKNYE ICE CHITVN
Sbjct: 429 VYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMICEACHITVN 488

Query: 482 KCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHR-EHGRLH 541
           K AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKAA ITKIVI R + G+LH
Sbjct: 489 KSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIRRGKLGKLH 548

Query: 542 KDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           KDIM NLQNNKDIVELRNRVE FA+SFAMPGFDI
Sbjct: 549 KDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of MC11g0673 vs. ExPASy TrEMBL
Match: A0A1S3B9K5 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=1)

HSP 1 Score: 1017 bits (2630), Expect = 0.0
Identity = 506/574 (88.15%), Postives = 528/574 (91.99%), Query Frame = 0

Query: 2   DLSLRFSSHATSSVSRSSIGDNSSYLCSTASNSSYPLQFHEQKDEKFCFVNGDADGHSED 61
           DLS R SSH TSS+SRSS GDNSSYLCST SNSSYP  F +QKDEKF  V G  D  S+D
Sbjct: 9   DLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKGVVDDDSDD 68

Query: 62  EKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQSLRVADPEI 121
           EKFSLLGQPLRVKRQRD  SFL+Q SAKR AV DE+SLE RRASV+ WGNQSLR+ADPEI
Sbjct: 69  EKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQSLRIADPEI 128

Query: 122 HEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQNIDEIE 181
           H IM KE++RQYKGIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQ IDEIE
Sbjct: 129 HNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDEIE 188

Query: 182 LLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSASGGHLSHGY 241
           LLCC RALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDSASGGHLSHGY
Sbjct: 189 LLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASGGHLSHGY 248

Query: 242 YTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 301
           Y+P GKKISA+SIFFESLPY+VNP TGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA
Sbjct: 249 YSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYA 308

Query: 302 RLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 361
           R RQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG
Sbjct: 309 RCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRG 368

Query: 362 LKSRKQGVLLTHGDDT--DTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQVASSEYG 421
           LKSRKQGVLLTHGDD    TYDFE+RINFSVFPSLQGGPHNNH+AALAIALKQVAS EY 
Sbjct: 369 LKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALKQVASPEYR 428

Query: 422 VYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICELCHITVN 481
           VYIRQVKKNAQALASALLRR CRLVT+GTDNHL+LWDLTPLGLTAKNYE ICE CHITVN
Sbjct: 429 VYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMICEACHITVN 488

Query: 482 KCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHR-EHGRLH 541
           K AIYGDNGAISPGGVRIGTPAMTTRGCLEADFET+AEILLKAA ITKIVI R + G+LH
Sbjct: 489 KSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIRRGKLGKLH 548

Query: 542 KDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 572
           KDIM NLQNNKDIVELRNRVE FA+SFAMPGFDI
Sbjct: 549 KDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of MC11g0673 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 817.8 bits (2111), Expect = 5.6e-237
Identity = 406/576 (70.49%), Postives = 470/576 (81.60%), Query Frame = 0

Query: 9   SHAT---SSVSRSSIGDNS-------SYLCSTASNSSYPLQFHEQKDEKFCFVN------ 68
           SHA+   +   R+ I D+S       S+     + S  PLQ  EQK EK   V+      
Sbjct: 17  SHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDG 76

Query: 69  GDADGHSEDEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSLEARRASVRAWGNQ 128
           G      EDE F +LG  + +KRQRD    L Q+   + +   +S LE+RRA+VRAWG+Q
Sbjct: 77  GGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQ 136

Query: 129 SLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT 188
            + +ADP+IHE+M KE+QRQ +GIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT
Sbjct: 137 PIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYT 196

Query: 189 GNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLDSA 248
           GNQ ID+IE LC ERAL AF L+S+KWGVNVQPYSCTSANFAVYTGLLLP +R+MGLDS 
Sbjct: 197 GNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSP 256

Query: 249 SGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICGGS 308
           SGGH+SHGY TPGGKKISA+SIFFES PYKVNPQTGY+DYDKLE+KALDYRPKILICGGS
Sbjct: 257 SGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGS 316

Query: 309 SYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPR 368
           SYPR+WD+AR+RQIADKCGAVLMCDMAHISGLVA KEC++PF++CDIVTSTTHK LRGPR
Sbjct: 317 SYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPR 376

Query: 369 GGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIALKQ 428
           GGIIF+RRG K RKQG   +H D +  YD EE+INF+VFPSLQGGPHNNHIAALAIALKQ
Sbjct: 377 GGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQ 436

Query: 429 VASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEKICE 488
           VA+ EY  YI+Q+KKNAQALA+ALLRRKCRLVT GTDNHL+LWDLTP+GLT K YEK+CE
Sbjct: 437 VATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCE 496

Query: 489 LCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIVIHR 548
           +CHIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFETMA+ L+KAAQIT   + R
Sbjct: 497 MCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITS-ALQR 556

Query: 549 EHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMP 569
           EHG+ HK+ + +L  NKDI ELRNRVEAFA  + MP
Sbjct: 557 EHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of MC11g0673 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 792.0 bits (2044), Expect = 3.3e-229
Identity = 397/593 (66.95%), Postives = 470/593 (79.26%), Query Frame = 0

Query: 2   DLSLRF-SSHA--TSSVSRSSIGD-------NSSYLCSTASNSSYPLQFHEQK------- 61
           DLSL F SSHA       R  I D       +SS+  S+      PLQ  EQ+       
Sbjct: 8   DLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVTGSC 67

Query: 62  -----DEKFCFVNGDADGHSEDEKFSLLGQPLRVKRQRDGKSFLNQASAKRVAVSDESSL 121
                ++     + D D   E+E+F LLG P+++KR R G S+   +S+       +S +
Sbjct: 68  SRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGI 127

Query: 122 EARRASVRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTN 181
           E+RRA VRAWGNQS+  ADPEIHE M KE+QRQ++GIELIASENFVCRAVMEALGSHLTN
Sbjct: 128 ESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTN 187

Query: 182 KYSEGMPGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGL 241
           KYSEGMPGARYYTGNQ ID+IE+LC ERALAAF L+ EKWGVNVQPYSCTSANFAV+TGL
Sbjct: 188 KYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGL 247

Query: 242 LLPKDRVMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKA 301
           L+P +R+MGLDS SGGH+SHGYYTPGGKK+S +SIFFES PYKV+P+TGY+DYDKLEEKA
Sbjct: 248 LMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKA 307

Query: 302 LDYRPKILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDI 361
           LDYRPKILICGGSSYPR+W++ R R IADKCGAVLM DMA ISGLVAAKE  +PF+YCDI
Sbjct: 308 LDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDI 367

Query: 362 VTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPH 421
           VTSTTHKSLRGPRGGIIF++RGLK +KQ + L H +    YDFEE+INFSVFPSLQGGPH
Sbjct: 368 VTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPH 427

Query: 422 NNHIAALAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTP 481
           NNHIAALAIALKQ AS EY +Y+RQVKKNA+ALASAL+ RKC+L+T GTDNHL+LWDLTP
Sbjct: 428 NNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTP 487

Query: 482 LGLTAKNYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEIL 541
           LGLT K YEK+CE+CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FETMA+ L
Sbjct: 488 LGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFL 547

Query: 542 LKAAQITKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 573
            +AAQI      REHG+L K+ + ++ + K+I +LRN+VEAFA+ FAMP FD+
Sbjct: 548 YRAAQIAS-AAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of MC11g0673 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 616.3 bits (1588), Expect = 2.5e-176
Identity = 296/465 (63.66%), Postives = 366/465 (78.71%), Query Frame = 0

Query: 106 VRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGM 165
           V +WGN SL   DPEIH+++ KE++RQ +GIELIASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 166 PGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDR 225
           PG RYY GN+ IDEIE LC  RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 226 VMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPK 285
           +MGLD  SGGHL+HGYYT GGKKISA+SI+FESLPYKVN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 286 ILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 345
           +LICGGS+YPR+WDYAR R IADK GA+L+CDMAHISGLVAA+E A+PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 346 KSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAA 405
           KSLRGPR G+IF+R+G K  K+G       +   YDFE++INF+VFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 406 LAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAK 465
           LA+ALKQ  +  + VY +QVK NA AL + L+ +  ++VT+GT+NHLVLWDL PLGLT  
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 466 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQI 525
             EK+C+LC IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE + E L +A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 526 TKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGF 571
           T + I + +G+L KD    L NNKD+ +L+  VE F++S+ MPGF
Sbjct: 424 T-LDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of MC11g0673 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 601.7 bits (1550), Expect = 6.3e-172
Identity = 287/467 (61.46%), Postives = 357/467 (76.45%), Query Frame = 0

Query: 106 VRAWGNQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGM 165
           V +WGN  L   DPEI++++ KE+ RQ +GIELIA+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 166 PGARYYTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDR 225
           PG RYY G + IDEIE LC  R+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 226 VMGLDSASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPK 285
           +MGLD  SGGH++HGYY+ GGK ISA+SI+FE+LPYKV+ +TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 286 ILICGGSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 345
           ++ICGG+SYPREWDYAR R +ADK GA L+CDMAH S LVAA+E A PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 346 KSLRGPRGGIIFFRRGLKSRKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAA 405
           KSLRGPR G+IF+R+G K  K+G       + + YDF+ +IN +VFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 406 LAIALKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAK 465
           LA+ALKQV +  + VY +QVK NA  LAS L+ +   LVTDGTDNHL+LWDL PLGLT  
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 466 NYEKICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQI 525
             EK+CELC+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE + E L +A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 526 TKIVIHREHGRLHKDIMNNLQNNKDIVELRNRVEAFASSFAMPGFDI 573
           T + I  ++G++ KD    L NNK+I E++  VE F   F MPGF I
Sbjct: 424 T-LDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of MC11g0673 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 479.2 bits (1232), Expect = 4.7e-135
Identity = 245/466 (52.58%), Postives = 317/466 (68.03%), Query Frame = 0

Query: 111 NQSLRVADPEIHEIMAKERQRQYKGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARY 170
           N  L   DPEI +I+  E+ RQ+KG+ELI SENF   +VM+A+GS +TNKYSEG PGARY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 171 YTGNQNIDEIELLCCERALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLLPKDRVMGLD 230
           Y GN+ ID  E LC +RAL AF LD EKWGVNVQP S + ANF VYT LL P +R+M LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 231 SASGGHLSHGYYTPGGKKISASSIFFESLPYKVNPQTGYVDYDKLEEKALDYRPKILICG 290
              GGHLSHGY T   KKISA SIFFE++PY+++  TGY+DYD++E+ A  +RPK+++ G
Sbjct: 172 LPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 291 GSSYPREWDYARLRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRG 350
            S+Y R +DYAR+R++ +K  AV++ DMAHISGLVAA    SPF+Y D+VT+TTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 351 PRGGIIFFRRGLKS-RKQGVLLTHGDDTDTYDFEERINFSVFPSLQGGPHNNHIAALAIA 410
           PRG +IFFR+G+K   KQG  +        YDFE++IN +VFP LQGGPHN+ I  LA+A
Sbjct: 292 PRGAMIFFRKGVKEINKQGKEV-------LYDFEDKINQAVFPGLQGGPHNHTITGLAVA 351

Query: 411 LKQVASSEYGVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLTPLGLTAKNYEK 470
           LKQ  +SEY  Y  QV  N+   A  L+ R   LV+ GTDNHLVL +L P G+     EK
Sbjct: 352 LKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEK 411

Query: 471 ICELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETMAEILLKAAQITKIV 530
           + E  HI  NK  + GD  A+ PGG+R+GTPA+T+RG +E DF  +AE   KA  I   V
Sbjct: 412 VLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKV 471

Query: 531 IHREHGRLHKDIMNNLQNN----KDIVELRNRVEAFASSFAMPGFD 572
                G   KD ++ ++++     +I +LR+ VE FA  F   GF+
Sbjct: 472 KSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509