MC11g0609 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0609 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.3e-34
Identity = 163/643 (25.35%), Postives = 258/643 (40.12%), Query Frame = 0

Query: 2   AGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEV-----DDKENHRGY 61
           A   ++L +   +  +DNG+L+V     DG+   +     + +  +     D  +N   Y
Sbjct: 25  ATGAVKLTLDGMNSTLDNGLLKVRFG-ADGSAKEVWKGGTNLISRLSGAARDPDKNRSFY 84

Query: 62  WDLVWNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIML 121
            D          +G  +E   +  EVI Q  +Q+ L++       D+   L ++   IM 
Sbjct: 85  LDYY--------SGGVNEFVPERLEVIKQTPDQVHLAY-----IDDQNGKLRLEYHLIMT 144

Query: 122 RNSSGFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHR 181
           R+ SG YSY +  +     P  + + R V+  R D      + ++ +R  PL  D L   
Sbjct: 145 RDVSGLYSYVVAANTGS-APVTVSELRNVY--RFDATRLDTLFNSIRRGTPLLYDELEQL 204

Query: 182 GKTLDFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEF 241
            K  D  E  +L +       G V  KY ++   ++ +   W  M    G W +  S E+
Sbjct: 205 PKVQD--ETWRLPD-------GSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEY 264

Query: 242 RSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGG 301
            SG  LKQ L  H     L     +H+   D+V +      ++K++GP  LY+N     G
Sbjct: 265 YSGDALKQELLVHQDAIILNYLTGSHFGTPDMVAQ----PGFEKLYGPWLLYINQ----G 324

Query: 302 DPLSLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDA 361
           +   L  D   +   E  SWPY +   +D   P QR  ++G+L          +T A  A
Sbjct: 325 NDRELVADVSRRAEHERASWPYRW--LDDARYPRQRATVSGRL----------RTEAPHA 384

Query: 362 YVGLAAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPG--FIGDYRYD 421
            V L +  E  ++  +  GY F  +T+ DG FS+ N   G+Y L A+  G   IG     
Sbjct: 385 TVVLNSSAE--NFDIQTTGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQ 444

Query: 422 VIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDR 481
            +     G    +G +    P   P  W IG  DR A EF             + D P +
Sbjct: 445 TV--RVEGKKTRLGQIDARQP--APLAWAIGQADRRADEF------------RFGDKPRQ 504

Query: 482 FRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV 541
           +R    W+        ADL + +G S   KDW++A              +W I F     
Sbjct: 505 YR----WQTEV----PADLTFEIGKSRERKDWYYAQTQ---------PGSWHILFNTRTP 564

Query: 542 DKNDAYTLRVALATANVAELQVNDPEAKPAVFTTGQI------GHDNTIARHGIH-GLYR 601
           ++   YTL +A+A A+   +       + AV   GQ+       +D +I R  +  G Y 
Sbjct: 565 EQ--PYTLNIAIAAASNNGMTTPASSPQLAVKLNGQLLTTLKYDNDKSIYRGAMQSGRYH 577

Query: 602 LYSVDMSGSLLVEGENTIFLSQIISSSSFNGVMYDYIRLEAPP 631
              + +    L +G N I L  +        VMYD I L   P
Sbjct: 625 EAHIPLPAGALQQGGNRITLELLGGM-----VMYDAITLTETP 577

BLAST of MC11g0609 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 6.0e-19
Identity = 109/460 (23.70%), Postives = 182/460 (39.57%), Query Frame = 0

Query: 120 SGFYSYAIYEHLKEWPPF--NLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRG 179
           +G ++++   +  E  P+  NL + R +F+   + + +  ++ +  +  PLP  +     
Sbjct: 131 TGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTH--LSSSEVQTAPLPSKKAVEEE 190

Query: 180 KTLDFPEAVKLVNPKEPQFKGEVD--DKYQYSCENKDLKVHGW-----ISMDPAVGFWQI 239
             +          P +  +    D   KY +S   +D  VHG       S     G W +
Sbjct: 191 VVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLV 250

Query: 240 TPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLN 299
             + +   GGPL  +LT     Y    +L +++ GE      N    + + FGP + + N
Sbjct: 251 MNTKDTYYGGPLHSDLTVDGIVY---NYLVSNHHGEGTP---NITYGFDRTFGPQYYHFN 310

Query: 300 SVTDGGDPLSLWKDAKTQMTKEVQSWPYSFPAS-----EDFPPPNQRGRLTGKLFVLDRY 359
                G   SL +      T    SW   F  S       + P +QRG + GK+ +    
Sbjct: 311 G--GKGSTASLQELKSDAETLADPSWNVDFYDSIAKHVVGYTPSSQRGSVQGKIKL---- 370

Query: 360 VSQDQTPATDAYVGLAAPGEAGSWQTEN-KGYQFWTKTDADGSFSIENARIGDYNLYAWV 419
                  AT     L   G+     + N   YQ+W + D  G FS+++ + G Y L  + 
Sbjct: 371 ----PKGATRPIAVLTVDGQYFQDNSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYA 430

Query: 420 PGFIGDYRYDVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEF-YVPDPDPNY 479
            G  GD+  D  V + AG    + +  +E    G  +W +G PD+S+ EF +    DP  
Sbjct: 431 DGIFGDFVRDG-VQVKAGKKTTIQE-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPT- 490

Query: 480 INKLYVDHPDRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVN---------RT 539
               +  HP  +  Y     +   +PD  + YT+G SD   D    H +         R 
Sbjct: 491 ----HPLHPPEYLIYWGAYDWQSDFPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPRV 550

Query: 540 RNDGSHLSTTWQIKFTV--DNVDKNDAYTLRVALATANVA 553
             D +H    W I F +  D++ +    TL + LA A  A
Sbjct: 551 EYDTTH---DWTINFPLSEDDLAERSKATLTIQLAGAKAA 561

BLAST of MC11g0609 vs. ExPASy Swiss-Prot
Match: B8NCU7 (Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglA PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.5e-09
Identity = 92/346 (26.59%), Postives = 139/346 (40.17%), Query Frame = 0

Query: 283 GPVFLYLNSVTDGGDPLSLW-----KDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTG 342
           GP F  +NS  +GG   SL+        +T+  ++    PYS   S    P         
Sbjct: 205 GPFFRDINS-NNGGSYNSLYWYMNSGHVQTEDRRQGLHGPYSMYFSRSGTPSTDIDTSFF 264

Query: 343 KLFVLDRYVSQDQTPATDAYVGLAAPGEAG-SWQTE--NKGYQFWTKTDADGSFSIENAR 402
               +  YV+ D         G A+  ++   W     N   Q+WT T +DGSF+    +
Sbjct: 265 ANLDIKGYVAAD---GRGTVSGTASGADSSFKWVVHWYNADAQYWTYTSSDGSFTSPAMK 324

Query: 403 IGDYNLYAWVPGFIGDYRY-DVIVSISAGS--DMDVGDLVYEPPRNGPTLWEIGIPDRSA 462
            GDY +      + G+Y+  +  VS++AGS    D+   V      G T+++IG  D + 
Sbjct: 325 PGDYTMVY----YQGEYKVAETSVSVTAGSSTSKDISGFV----ETGDTIFKIGDWDGTP 384

Query: 463 AEFYVPDPDPNYINKLYVDHPDRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHV 522
             F       N  N+L + HP   R    W           L YTVG S+ T D+  A  
Sbjct: 385 TGF------RNAENQLRM-HPSDSRM-SSW---------GPLTYTVGSSELT-DFPMAAF 444

Query: 523 NRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANVA---ELQVNDPEAKPAVFTT 582
               +  +       IKFT  +  +  A TLR+    +      +  +ND E       T
Sbjct: 445 KGVNDPVT-------IKFTATSA-QTGAATLRIGTTLSFAGGRPQATINDYEGSAPSAPT 504

Query: 583 GQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSS 615
               +   + R    GL  +Y V++    +VEGENTI +S I  SS
Sbjct: 505 NL--NSRGVTRGAYRGLGEVYDVNIPSGTIVEGENTITISVISGSS 510

BLAST of MC11g0609 vs. ExPASy Swiss-Prot
Match: Q2U0Q1 (Probable rhamnogalacturonate lyase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglA PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.6e-09
Identity = 89/346 (25.72%), Postives = 137/346 (39.60%), Query Frame = 0

Query: 283 GPVFLYLNSVTDGGDPLSLW-----KDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTG 342
           GP F  +NS  +GG   SL+        +T+  ++    PYS   S    P         
Sbjct: 205 GPFFRDINS-NNGGSYNSLYWYMNSGHVQTEDRRQGLHGPYSMYFSRSGTPSTDIDTSFF 264

Query: 343 KLFVLDRYVSQDQTPATDAYVGLAAPGEAG-SWQTE--NKGYQFWTKTDADGSFSIENAR 402
               +  YV+ D         G A+  ++   W     N   Q+WT T +DGSF+    +
Sbjct: 265 ANLDIKGYVATD---GRGTVSGTASGADSSFKWVVHWYNADAQYWTYTSSDGSFTSPAMK 324

Query: 403 IGDYNLYAWVPGFIGDYRY---DVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSA 462
            GDY +      + G+Y+     V V++ + +  D+   V      G T+++IG  D + 
Sbjct: 325 PGDYTMVY----YQGEYKVAETSVSVTVGSSTSKDISGSV----ETGDTIFKIGDWDGTP 384

Query: 463 AEFYVPDPDPNYINKLYVDHPDRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHV 522
             F       N  N+L + HP   R    W           L YTVG S+ T D+  A  
Sbjct: 385 TGF------RNAENQLRM-HPSDSRM-SSW---------GPLTYTVGSSELT-DFPMAAF 444

Query: 523 NRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANVA---ELQVNDPEAKPAVFTT 582
               +  +       IKFT  +  +  A TLR+    +      +  +ND E       T
Sbjct: 445 KGVNDPVT-------IKFTATSA-QTGAATLRIGTTLSFAGGRPQATINDYEGSAPSAPT 504

Query: 583 GQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSS 615
               +   + R    GL  +Y V++    +VEGENTI +S I  SS
Sbjct: 505 NL--NSRGVTRGAYRGLGEVYDVNIPSGTIVEGENTITISVISGSS 510

BLAST of MC11g0609 vs. NCBI nr
Match: XP_022136955.1 (probable rhamnogalacturonate lyase B [Momordica charantia])

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 631/633 (99.68%), Postives = 631/633 (99.68%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV
Sbjct: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL
Sbjct: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL
Sbjct: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY 540
           WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY
Sbjct: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY 540

Query: 541 TLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL 600
           TLRVALATANVAELQ  VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL
Sbjct: 541 TLRVALATANVAELQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL 600

Query: 601 VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS
Sbjct: 601 VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 633

BLAST of MC11g0609 vs. NCBI nr
Match: XP_008466141.1 (PREDICTED: probable rhamnogalacturonate lyase B [Cucumis melo])

HSP 1 Score: 1224 bits (3166), Expect = 0.0
Identity = 576/634 (90.85%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+GKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTSGTTGTFDEMRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LW+DAKTQM+ EVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRY+++++ PATDAYVGL
Sbjct: 301 LWEDAKTQMSIEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPGEAGSWQTENKGYQFWTKTDADGSFSIE+ RIGDYNLYAWVPGFIGDYRYDV++++S
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
            GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLYV+HPDRFRQYGL
Sbjct: 421 PGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540
           WERYAELYPDADLVY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF VDN+ DK+  
Sbjct: 481 WERYAELYPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGT 540

Query: 541 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600
           YTLRVALATANVAELQ  VN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEGENTIFLSQII+SSSFNGVMYDYIRLE PPS
Sbjct: 601 LVEGENTIFLSQIINSSSFNGVMYDYIRLEGPPS 634

BLAST of MC11g0609 vs. NCBI nr
Match: XP_038899392.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1222 bits (3161), Expect = 0.0
Identity = 579/634 (91.32%), Postives = 611/634 (96.37%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+GKEFEVIVQNEEQIELSFKRNY FKD+AVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYPFKDEAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEH+KEWPPFNLPQTRIVFKLRKDKFHYMAIAD+R+RFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHMKEWPPFNLPQTRIVFKLRKDKFHYMAIADDRRRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPKFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DG DPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGVDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LW+DAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV+QD+  ATDAYVGL
Sbjct: 301 LWEDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNQDKMLATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPG+AGSWQTENKGYQFWTKTDADGSFSIE+ RIGDYNLYAWVPGFIGDYRYD ++S+S
Sbjct: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFSIEHIRIGDYNLYAWVPGFIGDYRYDAVISVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDP YINKLYV+HPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540
           WE+YAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHL+TTW+IKFT+DNV +K+  
Sbjct: 481 WEKYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVIEKSGT 540

Query: 541 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600
           YTLRVALATANVAELQ  VN+PEAKP++FTTG IGHDNTIARHGIHGLYRLYSVD+ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNTIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEG+NTIFLSQII++SSFNGVMYDYIRLE PPS
Sbjct: 601 LVEGDNTIFLSQIINNSSFNGVMYDYIRLEGPPS 634

BLAST of MC11g0609 vs. NCBI nr
Match: XP_004136251.1 (probable rhamnogalacturonate lyase B [Cucumis sativus] >KGN60274.1 hypothetical protein Csa_002470 [Cucumis sativus])

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 567/634 (89.43%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+G+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLK++PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LW+DAKTQM+KEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV++++ PATDAYVGL
Sbjct: 301 LWEDAKTQMSKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPG+ GSWQTE KGYQFWTKTDADGSFSIE+ RIGDYNLYAWVPGFIGDYRYDV++++S
Sbjct: 361 AAPGDVGSWQTEKKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
           +GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLY++HPDRFRQYGL
Sbjct: 421 SGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540
           WE+YAEL+PDADLVY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF +DN+ DK  +
Sbjct: 481 WEKYAELHPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVS 540

Query: 541 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600
           YTLRVALATANVAELQ  VN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEGENTIFLSQII++SSFNG+MYDYIRLE PPS
Sbjct: 601 LVEGENTIFLSQIINNSSFNGIMYDYIRLEGPPS 634

BLAST of MC11g0609 vs. NCBI nr
Match: XP_023535230.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1174 bits (3036), Expect = 0.0
Identity = 557/634 (87.85%), Postives = 592/634 (93.38%), Query Frame = 0

Query: 5   ILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSG 64
           ILQL+I +HHVVMDNGIL VTLSKPDGNVTGIKY+SIDNVLEV DKE HRGYWDLVWNSG
Sbjct: 6   ILQLDIHDHHVVMDNGILAVTLSKPDGNVTGIKYSSIDNVLEVRDKEAHRGYWDLVWNSG 65

Query: 65  TGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYS 124
           T GTTGTFDEM+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFYS
Sbjct: 66  TPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFYS 125

Query: 125 YAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPE 184
           YAIYEHL+EWPPFNLPQTRIVFKLRKDKFHYMAIADNR+R+MPLPEDR+  RGKTLDFPE
Sbjct: 126 YAIYEHLREWPPFNLPQTRIVFKLRKDKFHYMAIADNRKRWMPLPEDRVGRRGKTLDFPE 185

Query: 185 AVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSGG 244
           AV LV+P+E  FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSGG
Sbjct: 186 AVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSGG 245

Query: 245 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 304
           PLKQNLTSHVGPYALAMFLSAHY GE+LVLKLNPDEPWKKVFGPVF YLNSV +G DPLS
Sbjct: 246 PLKQNLTSHVGPYALAMFLSAHYGGEELVLKLNPDEPWKKVFGPVFFYLNSVPNGTDPLS 305

Query: 305 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 364
           LW+DAK QMTKEVQSWPYSFPASE+FPPPNQRGRLTG LFVLDRY  Q+Q PA +AYVGL
Sbjct: 306 LWEDAKIQMTKEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYAKQEQMPAAEAYVGL 365

Query: 365 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 424
           AAPG+AGSWQTE KGYQFWTKTDA GSFSIEN RIGDYNLYAWVPGFIGDYRYDV++S+S
Sbjct: 366 AAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVS 425

Query: 425 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 484
           AGSDMDVGD V+EPPRNGPTLWEIGIPDRSAAEFY+PDP+PNYINKLYVDHPDRFRQYGL
Sbjct: 426 AGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVDHPDRFRQYGL 485

Query: 485 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDN-VDKNDA 544
           WERYAELYPD DLVYTVGVSDYTKDWFFAHVNRTRNDGSHL TTWQIKFT+DN +DK+  
Sbjct: 486 WERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGI 545

Query: 545 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 604
           Y LRVALATANVAELQ  VN+PE + A+FTTGQIGHDN IARHGIHGLYRLYSVD+SGS+
Sbjct: 546 YKLRVALATANVAELQIRVNNPEVESALFTTGQIGHDNAIARHGIHGLYRLYSVDISGSV 605

Query: 605 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEGENTI LSQII++SSFNGVMYDYIRLE PPS
Sbjct: 606 LVEGENTILLSQIINNSSFNGVMYDYIRLEGPPS 639

BLAST of MC11g0609 vs. ExPASy TrEMBL
Match: A0A6J1C5C7 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3 SV=1)

HSP 1 Score: 1317 bits (3408), Expect = 0.0
Identity = 631/633 (99.68%), Postives = 631/633 (99.68%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV
Sbjct: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL
Sbjct: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL
Sbjct: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY 540
           WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY
Sbjct: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAY 540

Query: 541 TLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL 600
           TLRVALATANVAELQ  VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL
Sbjct: 541 TLRVALATANVAELQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLL 600

Query: 601 VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS
Sbjct: 601 VEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 633

BLAST of MC11g0609 vs. ExPASy TrEMBL
Match: A0A1S3CQT8 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1)

HSP 1 Score: 1224 bits (3166), Expect = 0.0
Identity = 576/634 (90.85%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+GKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTSGTTGTFDEMRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LW+DAKTQM+ EVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRY+++++ PATDAYVGL
Sbjct: 301 LWEDAKTQMSIEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPGEAGSWQTENKGYQFWTKTDADGSFSIE+ RIGDYNLYAWVPGFIGDYRYDV++++S
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
            GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLYV+HPDRFRQYGL
Sbjct: 421 PGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540
           WERYAELYPDADLVY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF VDN+ DK+  
Sbjct: 481 WERYAELYPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGT 540

Query: 541 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600
           YTLRVALATANVAELQ  VN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEGENTIFLSQII+SSSFNGVMYDYIRLE PPS
Sbjct: 601 LVEGENTIFLSQIINSSSFNGVMYDYIRLEGPPS 634

BLAST of MC11g0609 vs. ExPASy TrEMBL
Match: A0A0A0LGY0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=1)

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 567/634 (89.43%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+G+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLK++PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360
           LW+DAKTQM+KEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV++++ PATDAYVGL
Sbjct: 301 LWEDAKTQMSKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGL 360

Query: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420
           AAPG+ GSWQTE KGYQFWTKTDADGSFSIE+ RIGDYNLYAWVPGFIGDYRYDV++++S
Sbjct: 361 AAPGDVGSWQTEKKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480
           +GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLY++HPDRFRQYGL
Sbjct: 421 SGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPDRFRQYGL 480

Query: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540
           WE+YAEL+PDADLVY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF +DN+ DK  +
Sbjct: 481 WEKYAELHPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVS 540

Query: 541 YTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600
           YTLRVALATANVAELQ  VN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           LVEGENTIFLSQII++SSFNG+MYDYIRLE PPS
Sbjct: 601 LVEGENTIFLSQIINNSSFNGIMYDYIRLEGPPS 634

BLAST of MC11g0609 vs. ExPASy TrEMBL
Match: A0A6J1FDW4 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 SV=1)

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 553/635 (87.09%), Postives = 591/635 (93.07%), Query Frame = 0

Query: 4   AILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNS 63
           +ILQL+I +HHVVMDNGIL VTLSKP GNVTGIKY+SIDNVLEV DKE HRGYWDLVWNS
Sbjct: 5   SILQLDIHDHHVVMDNGILAVTLSKPYGNVTGIKYSSIDNVLEVRDKEAHRGYWDLVWNS 64

Query: 64  GTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFY 123
           GT GTTGTFDEM+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFY
Sbjct: 65  GTPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFY 124

Query: 124 SYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFP 183
           SYAIYEHL+EWPPFNLPQTRIVFKLRKDKFHYMAIADNR+R MPLP+DR+  RGKTLDFP
Sbjct: 125 SYAIYEHLREWPPFNLPQTRIVFKLRKDKFHYMAIADNRKRSMPLPDDRVGGRGKTLDFP 184

Query: 184 EAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSG 243
           EAV LV+P+E  FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSG
Sbjct: 185 EAVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSG 244

Query: 244 GPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPL 303
           GPLKQNLTSHVGPYALAMFLSAHY GE+LVLKLN DEPWKKVFGPVF YLNSV +G DPL
Sbjct: 245 GPLKQNLTSHVGPYALAMFLSAHYGGEELVLKLNADEPWKKVFGPVFFYLNSVPNGRDPL 304

Query: 304 SLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVG 363
            LW+DAK QMTKEVQSWPYSFPAS++FPPPNQRGRLTG L+VLD YV+Q+Q PA +AYVG
Sbjct: 305 CLWEDAKIQMTKEVQSWPYSFPASQNFPPPNQRGRLTGNLYVLDTYVNQEQMPAAEAYVG 364

Query: 364 LAAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSI 423
           LAAPG+AGSWQTE KGYQFWTKTDA GSFSIEN RIGDYNLYAWVPGFIGDYRYDV++S+
Sbjct: 365 LAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISV 424

Query: 424 SAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYG 483
           SAGSDMDVGDLV+EPPRNGPTLWEIGIPDRSAAEFY+PDP+PNYINKLYV HPDRFRQYG
Sbjct: 425 SAGSDMDVGDLVFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVHHPDRFRQYG 484

Query: 484 LWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDN-VDKND 543
           LWERYAELYPD DLVYTVGVSDYTKDWFFAHVNRTRNDGSHL TTWQIKFT+DN +DK+ 
Sbjct: 485 LWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSG 544

Query: 544 AYTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGS 603
            Y LRVALATANVAELQ  VN+PE +PA+FTTGQIGHDN IARHGIHGLYRLYSVDMS S
Sbjct: 545 IYKLRVALATANVAELQIRVNNPEVEPALFTTGQIGHDNAIARHGIHGLYRLYSVDMSRS 604

Query: 604 LLVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           +LVEGENTIFLSQII++SSFNGVMYDYIRLE PPS
Sbjct: 605 VLVEGENTIFLSQIINTSSFNGVMYDYIRLEGPPS 639

BLAST of MC11g0609 vs. ExPASy TrEMBL
Match: A0A6J1IFC5 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV=1)

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 551/635 (86.77%), Postives = 590/635 (92.91%), Query Frame = 0

Query: 4   AILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNS 63
           A LQL+I +HHVVMDNGIL VTLSKPDGNVTGIKY++IDN+LEV DKE HRGYWDLVWNS
Sbjct: 5   ATLQLDIHDHHVVMDNGILAVTLSKPDGNVTGIKYSNIDNILEVRDKEAHRGYWDLVWNS 64

Query: 64  GTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFY 123
           GT GTTGTFDEM+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFY
Sbjct: 65  GTPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFY 124

Query: 124 SYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFP 183
           SYAIYEHL+EWPPFNLPQTRIVFKL KDKFHYMAIADNR+R MPLP+DR+  RGKTLDFP
Sbjct: 125 SYAIYEHLREWPPFNLPQTRIVFKLSKDKFHYMAIADNRKRSMPLPDDRVGRRGKTLDFP 184

Query: 184 EAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSG 243
           EAV LV+P+E  FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSG
Sbjct: 185 EAVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSG 244

Query: 244 GPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPL 303
           GPLKQNLTSHVGPYALAMFLSAHY GE+LVLK+NPDEPWKKVFGPVF YLNSV +G DPL
Sbjct: 245 GPLKQNLTSHVGPYALAMFLSAHYGGEELVLKMNPDEPWKKVFGPVFFYLNSVPNGTDPL 304

Query: 304 SLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVG 363
           SLW+DAKTQMTKEVQSWPYSFPASE+FPPPNQRGRLTG LFVLDRYV+Q+Q PA +AYVG
Sbjct: 305 SLWEDAKTQMTKEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYVNQEQMPAAEAYVG 364

Query: 364 LAAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSI 423
           LAAPG+AGSWQTE KGYQFWTKTDA GSFSIEN RIGDYNLYAWVPGFIGDY YDV++S+
Sbjct: 365 LAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYHYDVVISV 424

Query: 424 SAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYG 483
           SAGSD+D+GDLV+EPPRNGPT+WEIGIPDRSA EFY+PDP+PNYINKLYVDHPDRFRQYG
Sbjct: 425 SAGSDIDIGDLVFEPPRNGPTVWEIGIPDRSANEFYIPDPNPNYINKLYVDHPDRFRQYG 484

Query: 484 LWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDN-VDKND 543
           LWERYAELYPD DLVYTVGVSDY KDWFFAHVNRTRNDGSHL TTWQIKFT+DN VDK+ 
Sbjct: 485 LWERYAELYPDTDLVYTVGVSDYPKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNVDKSG 544

Query: 544 AYTLRVALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGS 603
            Y LRVALATANVAELQ  VN+ E + A+FTTGQIGHDN IARHGIHGLYRLYSVDMSGS
Sbjct: 545 IYKLRVALATANVAELQIRVNNLEVEHALFTTGQIGHDNAIARHGIHGLYRLYSVDMSGS 604

Query: 604 LLVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPS 631
           +LVEGENTIFLSQII+ SSFNGVMYDYIRLE PPS
Sbjct: 605 VLVEGENTIFLSQIINDSSFNGVMYDYIRLEGPPS 639

BLAST of MC11g0609 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 884.4 bits (2284), Expect = 5.4e-257
Identity = 413/634 (65.14%), Postives = 511/634 (80.60%), Query Frame = 0

Query: 6   LQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVW-NSG 65
           +QL++Q  HVVM NG ++VT+SKPDG VTGI Y  +DN+LE  +++ +RGYWDLVW + G
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  TGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGF 125
           T GTTG  + +KG  FEV+V+NEE +E+SF R +  S +D   P+N+DKRFIM ++ +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDF 185
           YSYAI+EHL EWP FNLPQTRIV+KLRKDKF YMAIADNRQR MPLPEDRL  RG+ L +
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPL 245
           PEAV LV+P E +FKGEVDDKY+YS ENKDLKVHGWIS +  +G WQI PS+EFRSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTD-GGDPLSL 305
           KQNLTSHVGP +LAMFLSAHY+GED+V+K+   + WKKVFGPVF YLN + D   DPLSL
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLA 365
           W+DAK QM  EVQSWPY FPASEDFP  ++RG ++G+L V D+++S D  PA  A+VGLA
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 APGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISA 425
            PGE GSWQ E+KGYQFWT+ D+DG F+I + R G+YNL  +V G+IGDY+Y+ +++I+A
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGLW 485
           G D+DVG++VYEPPR+GPT+WEIGIPDRSAAEF+VPDP+P YINKLY+ HPDRFRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 ERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYT 545
           ERY ELYP  DLV+T+GVSDY KDWFFAHV R   D ++  TTWQIKF ++NV K+  Y 
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYK 546

Query: 546 LRVALATANVAELQV----NDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 605
           +R+ALATANVAELQV    +D E    +FTTG IGHDN IARHGIHG+YRLY+VD+    
Sbjct: 547 IRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEK 606

Query: 606 LVEGENTIFLSQIISSS-SFNGVMYDYIRLEAPP 631
           LVEG+NT+FL+Q ++++ +FNG+MYDYIRLE PP
Sbjct: 607 LVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPP 640

BLAST of MC11g0609 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 876.3 bits (2263), Expect = 1.5e-254
Identity = 420/619 (67.85%), Postives = 497/619 (80.29%), Query Frame = 0

Query: 17  MDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDEMK 76
           MDNGI +VTLSKPDG VTGI+YN IDN+LEV ++E +RGYWDLVW  G  GT G FD +K
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVW--GGSGTAGGFDVIK 60

Query: 77  GKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 136
           G  FEVIV+NEEQIELSF R +  S + KAVPLNIDKRF+ML  SSGFY+YAIYEHLKEW
Sbjct: 61  GSNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEW 120

Query: 137 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 196
           P F+L +TRI FKLRK+KFHYMA+ D+RQRFMPLP+DRL  RG+ L +PEAV LVNP E 
Sbjct: 121 PAFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLES 180

Query: 197 QFKGEVDDKYQYSCENKDLKVHGWISMD-PAVGFWQITPSSEFRSGGPLKQNLTSHVGPY 256
           QFKGEVDDKYQYSCENKD+ VHGWI  + P+VGFW ITPS E+R+GGP KQNLTSHVGP 
Sbjct: 181 QFKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPT 240

Query: 257 ALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVT-DGGDPLSLWKDAKTQMTKE 316
           ALA+F+SAHY+GEDLV K +  E WKKVFGPVF+YLNS T D  DPL LW+DAK+QM  E
Sbjct: 241 ALAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVE 300

Query: 317 VQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTE 376
            +SWPYSFPAS+D+    QRG + G+L V DRYV +D   A   YVGLA PG AGSWQ E
Sbjct: 301 AESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRE 360

Query: 377 NKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVY 436
            K YQFWT+TD +G F I   R G YNLYAW+PGFIGDY+YD +++I++G  + V DLVY
Sbjct: 361 CKEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVY 420

Query: 437 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGLWERYAELYPDAD 496
           +PPRNG TLWEIG PDRSAAEFYVPDP+P YIN LY +HPDRFRQYGLWERYAELYPD D
Sbjct: 421 QPPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKD 480

Query: 497 LVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANVA 556
           LVY VG SDY KDWF+A V R +++ ++  TTWQIKF + N+DKN +YTLRVA+A+A  +
Sbjct: 481 LVYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFS 540

Query: 557 ELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 616
           ELQ  VN+  A P +FT+G IG DN+IARHGIHGLY L++V+++GS L+EGENT+FL+Q 
Sbjct: 541 ELQIRVNNANASP-MFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQP 600

Query: 617 ISSSSFNGVMYDYIRLEAP 630
            S+S F G+MYDYIR EAP
Sbjct: 601 RSTSPFQGIMYDYIRFEAP 616

BLAST of MC11g0609 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 837.8 bits (2163), Expect = 5.7e-243
Identity = 391/630 (62.06%), Postives = 483/630 (76.67%), Query Frame = 0

Query: 6   LQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGT 65
           + L++ + +VVMDNGILQVTLSKP G +TGI+YN IDNVLEV +KE +RGYWDL WN   
Sbjct: 49  VHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLHWNE-- 108

Query: 66  GGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFY 125
            G  G FD + G  F VIV+ EEQ+E+SF R +  S + K +PLNIDKRFIMLR SSG Y
Sbjct: 109 PGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSSGVY 168

Query: 126 SYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFP 185
           SY IYEHLK+WP F L +TRI FKLRKDKFHYMA+AD+R+R MP P+D  + R +TLD+ 
Sbjct: 169 SYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQ 228

Query: 186 EAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLK 245
           EA  L  P +P+ +GEVDDKYQYSCENKDL+VHGWIS DP VGFWQITPS+EFRSGGPLK
Sbjct: 229 EASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLK 288

Query: 246 QNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWK 305
           QNLTSHVGP  LA+F S HY+G+ ++ +    EPWKKV+GPVF+YLNS  +G DPL LW 
Sbjct: 289 QNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWD 348

Query: 306 DAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAP 365
           DAK +M  EV+ WPYSF AS+D+P   +RG   G+L + DR+++ D   A  AYVGLA P
Sbjct: 349 DAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPP 408

Query: 366 GEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGS 425
           G++GSWQ E KGYQFW   D  G FSI N R G+YNLYAWVP FIGDY    IV +++G 
Sbjct: 409 GDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGC 468

Query: 426 DMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGLWER 485
            +++GD+VYEPPR+GPTLWEIGIPDR A+EF++PDPDP  +N++ V H DRFRQYGLW++
Sbjct: 469 MIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGLWKK 528

Query: 486 YAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLR 545
           Y ++YP+ DLVYTVGVSDY +DWFFAHV R + D  H  TTWQI F ++N+D+   Y LR
Sbjct: 529 YTDMYPNDDLVYTVGVSDYRRDWFFAHVPRKKGD-VHEGTTWQIIFNLENIDQKANYKLR 588

Query: 546 VALATANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEG 605
           VA+A+A +AELQ  +ND EA   +FTTG IG DN+IARHGIHG+Y LY+V++ G+ LV+G
Sbjct: 589 VAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQG 648

Query: 606 ENTIFLSQIISSSSFNGVMYDYIRLEAPPS 632
           +NTIFL Q   +  F G+MYDYIRLE PPS
Sbjct: 649 DNTIFLKQPRCNGPFQGIMYDYIRLEGPPS 675

BLAST of MC11g0609 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 798.1 bits (2060), Expect = 5.0e-231
Identity = 379/624 (60.74%), Postives = 473/624 (75.80%), Query Frame = 0

Query: 17  MDNGILQVTLSKPDGNVTGIKYNSIDNVLE-VDDKENHRGYWDLVWN---SGTGGTTGTF 76
           M+N  LQ+TLS P+G VTGI+YN IDNVL    +KE  RGYWD+VWN        T GT 
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 77  DEMKGKEFEVIVQNEEQIELSFKRNYSFKD-KAVPLNIDKRFIMLRNSSGFYSYAIYEHL 136
           D ++  + EVI QN+E+IELSF R ++     AVP+NIDKRF+ML+NSSGFYSYAI+E L
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTSSTTAVPVNIDKRFVMLQNSSGFYSYAIFERL 120

Query: 137 KEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNP 196
           + WP   L   R+VFKL K KFHYMAI+D+RQR+MP+P+DR+  RG+ L +PEAV+L++P
Sbjct: 121 QGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLDP 180

Query: 197 KEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVG 256
            EP+FKGEVDDKY+YS E+KD+KVHGWIS + +VGFWQITPS+EFRS GPLKQ L SHVG
Sbjct: 181 IEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVG 240

Query: 257 PYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTK 316
           P  LA+F S HY G DL++     E WKKVFGPVF+YLNS   G DPL LW +AK Q   
Sbjct: 241 PTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKI 300

Query: 317 EVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQT 376
           E + WPY+F AS+DFP  +QRG ++G+L V DR++S +  PA  +YVGLAAPG+ GSWQ 
Sbjct: 301 EEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQR 360

Query: 377 ENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLV 436
           E KGYQFW+K D +GSFSI N R G YNLYA+ PGFIGDY  D +  IS GS + +GDLV
Sbjct: 361 ECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLV 420

Query: 437 YEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGLWERYAELYPDA 496
           YEPPR+G TLWEIG+PDRSAAEFY+PDP+P+++NKLY++H D++RQYGLWERY+ELYPD 
Sbjct: 421 YEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPDE 480

Query: 497 DLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKN--DAYTLRVALATA 556
           D+VY V + DY+K+WFF  V R + +G +  TTWQI+F  D+  KN    + LR+ALAT+
Sbjct: 481 DMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALATS 540

Query: 557 NVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFL 616
           NVAELQ  VND  A P +F T QIG DNTIARHGIHGLY LYSV++  + L  G NTI+L
Sbjct: 541 NVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIYL 600

Query: 617 SQIISSSSFNGVMYDYIRLEAPPS 632
           +Q +++S F G+MYDYIRLE P S
Sbjct: 601 TQALATSPFQGLMYDYIRLEYPDS 624

BLAST of MC11g0609 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 755.4 bits (1949), Expect = 3.8e-218
Identity = 369/620 (59.52%), Postives = 454/620 (73.23%), Query Frame = 0

Query: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 74
           VV+DNGI+QVT S P+G +TGIKY+ IDNVL  DDK + RGYWD+VW           D+
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVL--DDKIDDRGYWDVVWYEPE--KKQKTDK 121

Query: 75  MKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLK 134
           ++G +FE+I QNEEQIE+SF R +  S +   VPLN+DKR+I+    SG Y Y I E L+
Sbjct: 122 LEGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLE 181

Query: 135 EWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPK 194
            WP  ++ Q RIVFKL   KF +MAI+D+RQR MP   D  R   K+L + EAV L NP 
Sbjct: 182 GWPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMAD--RENSKSLAYKEAVLLTNPS 241

Query: 195 EPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGP 254
            P FKGEVDDKY YS E+KD  VHGWIS DP VGFW ITPS EFR GGP+KQ+LTSH GP
Sbjct: 242 NPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGP 301

Query: 255 YALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKE 314
             L+MF S HY+G+++ +     EPWKKVFGPV  YLNSV+     L LW+DAK QM  E
Sbjct: 302 ITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAE 361

Query: 315 VQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTE 374
           V+SWPY F  SED+P  +QRG L G+  + D YVS+ +     A+VGLA  GEAGSWQTE
Sbjct: 362 VKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTE 421

Query: 375 NKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVY 434
           +KGYQFWTK D  G F IEN R G+Y+LYAW  GFIGDY+Y+  ++I+ GS+M+VG LVY
Sbjct: 422 SKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVY 481

Query: 435 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDH-PDRFRQYGLWERYAELYPDA 494
           EPPRNGPTLWEIG+PDR+A EFY+PDP P  +NKLYV+   DRFRQYGLW+RYA+LYP  
Sbjct: 482 EPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQN 541

Query: 495 DLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANV 554
           DLVYT+GVSDY  DWFFAHV R   + ++  TTWQI F + NV++   YTLR+ALA+A  
Sbjct: 542 DLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAAD 601

Query: 555 AELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQ 614
           +ELQ  +NDP++  A+FTTG IG DN IARHGIHGLYRLYS+D++G+LL  G+NTIFL+Q
Sbjct: 602 SELQIRINDPKS-DAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQ 661

Query: 615 IISSSSFNGVMYDYIRLEAP 630
             S + F G+MYDYIRLE+P
Sbjct: 662 TRSRTPFQGIMYDYIRLESP 674