MC11g0526 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0526
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Sarcosine oxidase
Location	MC11: 4077472 .. 4079569 (-)
RNA-Seq Expression	MC11g0526
Synteny	MC11g0526

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAGATTGGGAGCATGACAGGCTTTTGCTTTTATTAAAATATATTTTTTTTCGTAATACGAAATAATCAATACTTTAAAATTTTATTTTTCTATTTATTTCATTATTTTCCCTTTTACTTTTCAAATGACTAGAGTTTCCCTTTACTTCTCTGCTTCTTCTTTTAACATTAACATTTCGAATCTTCTTCTCTTCTTCTACCAAGTCTAATTCTTCTCTTCTCCTGCATCTTCTTTCTTTCTTAGTTCTGGACCGAATGGCACCATCGATTTCGGTTCGGTTCGATTTATAAAGTATTTCTTTTTTATTTCTTTACATTAAAAATTCGTTTGGGTTTCGGTTCGGCCGTGAACACTAATTTGTGAAAGAAGAAAAAAGGGAATTGAGAAAGGGCATTATCGTCATTTTATCCTAAACTCTGACAACTTCCAAGAAGCCTCTGGGTTTCAATTTTAAACTCCCTTCTGTTGGTATTGAGTATTGACCATAAACACATCGTTGCTCACGCGCCGGTTCCCCTCATCGTAAACTCCGACGGAGAGAAATGGAAGATTCCGTTAGTCAATTCGACGTCATCGTCGTCGGCGGCGGCGTAATGGGCAGTTCAACGGCGTACCATCTCGCGAAAAGAGGAAACAGAGTTGTGATTCTGGAGCAGTTCGATTTCCTCCACCACCGAGGGTCTTCCCACGGCGAGTCACGCACCATACGCGCCACCTACCCGGAGGACTACTACCACCGTCTGGTCATGGACTCCTACGACCTCTGGCGGGCCGCCGAGTTGGAAATCGGCTACCGGGTCTATTTTCCGGCGGAGCAGCTCGATTTCGGCCCATCGGACGACAAAAGTCTCGCTGCCGTCATCGACACCAGCCAGAAACTTTCGATTCCCCATCGGGTCCTCGACCACCGGCAACTAGCGGAGAAATACTCCGGCAGGGTGGCGATTCCGGCGGATTGGGTGGCGGTGTGGAGCAAGCACGGCGGCGTAATCAAGCCCACCAAGGCGGTGGCTATGTTCCAAACTTTGGCGTACAAAAACGGAGCCGTTTTGAAGGACAATTCAGAGGTGGTGGCAATAAAGAGGGATGAGGGTAGTGGAGGAATTGTTGTTTCCACAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACGGTTGGAGCTTGGGCTGCAAAGTTAGTTAAATTAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGACTACTGTTTGCTACTGGCGGATCAAGGAGGGGGCCGAGGCCGAGTACGCCATCGGAGGGGATTTCCCGACGTTCGCCAGCTACGGCGACCCGTACATTTACGGGACGCCGTCGCTGGAGTTCCCAGGGCTGATGAAGGTAGCGGTGCACGGCGGGCATCCATGCGATCCCGACAAGCGGCCGTGGAGGGGCGGGGAGCGGCTACCGGTGGTAGCATTGAAGGAGTGGATTGAGGGGAGATTTGGGGGGGCAGTGGATTCAAGCAAGCCTGTGGCAACGCAGCTGTGTATGTACTCAATGACACCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGGCAAGGATGTGGTGATCGGCGGCGGGTTCTCGGGGCACGGGTTCAAAATGTCACCGGCGATCGGGAGAGTGCTGGCAGATCTTGCGGTGGACGGGACGGCGGAAAGGGTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGGAATTTCAAGAGCTTTTTAGACCAAGTAAAAGCATCACCACTATAGCTCCTCTGATTCGTTTATGGGTTTCATGCTTATTCTCTCGTCTCTTCCATCGGTAATATGTTCATTTAGTTTTTGAATTTTGGTAATTATGTTTAATAATTTCAATTGCTTTGTTTAGTTTATGCTTCCTACTCATCTAAATTATGGTGTATGCTTCCTACTCATCTAAATTATGGTGTTCTTGCTGATTCAATATGCTTCATACTCATCTAAATTATGGTGTTCTTGCTAATTCAAGAATAGCTCAACAAATAATAAAAAAAAAATTGTATTTCTATTTTTATTTGCTCGGTAAGAATAAGATGTGTGGTTGAAGAG

mRNA sequence

CCCAGATTGGGAGCATGACAGGCTTTTGCTTTTATTAAAATATATTTTTTTTCGTAATACGAAATAATCAATACTTTAAAATTTTATTTTTCTATTTATTTCATTATTTTCCCTTTTACTTTTCAAATGACTAGAGTTTCCCTTTACTTCTCTGCTTCTTCTTTTAACATTAACATTTCGAATCTTCTTCTCTTCTTCTACCAAGTCTAATTCTTCTCTTCTCCTGCATCTTCTTTCTTTCTTAGTTCTGGACCGAATGGCACCATCGATTTCGGTTCGGTTCGATTTATAAAGTATTTCTTTTTTATTTCTTTACATTAAAAATTCGTTTGGGTTTCGGTTCGGCCGTGAACACTAATTTGTGAAAGAAGAAAAAAGGGAATTGAGAAAGGGCATTATCGTCATTTTATCCTAAACTCTGACAACTTCCAAGAAGCCTCTGGGTTTCAATTTTAAACTCCCTTCTGTTGGTATTGAGTATTGACCATAAACACATCGTTGCTCACGCGCCGGTTCCCCTCATCGTAAACTCCGACGGAGAGAAATGGAAGATTCCGTTAGTCAATTCGACGTCATCGTCGTCGGCGGCGGCGTAATGGGCAGTTCAACGGCGTACCATCTCGCGAAAAGAGGAAACAGAGTTGTGATTCTGGAGCAGTTCGATTTCCTCCACCACCGAGGGTCTTCCCACGGCGAGTCACGCACCATACGCGCCACCTACCCGGAGGACTACTACCACCGTCTGGTCATGGACTCCTACGACCTCTGGCGGGCCGCCGAGTTGGAAATCGGCTACCGGGTCTATTTTCCGGCGGAGCAGCTCGATTTCGGCCCATCGGACGACAAAAGTCTCGCTGCCGTCATCGACACCAGCCAGAAACTTTCGATTCCCCATCGGGTCCTCGACCACCGGCAACTAGCGGAGAAATACTCCGGCAGGGTGGCGATTCCGGCGGATTGGGTGGCGGTGTGGAGCAAGCACGGCGGCGTAATCAAGCCCACCAAGGCGGTGGCTATGTTCCAAACTTTGGCGTACAAAAACGGAGCCGTTTTGAAGGACAATTCAGAGGTGGTGGCAATAAAGAGGGATGAGGGTAGTGGAGGAATTGTTGTTTCCACAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACGGTTGGAGCTTGGGCTGCAAAGTTAGTTAAATTAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGACTACTGTTTGCTACTGGCGGATCAAGGAGGGGGCCGAGGCCGAGTACGCCATCGGAGGGGATTTCCCGACGTTCGCCAGCTACGGCGACCCGTACATTTACGGGACGCCGTCGCTGGAGTTCCCAGGGCTGATGAAGGTAGCGGTGCACGGCGGGCATCCATGCGATCCCGACAAGCGGCCGTGGAGGGGCGGGGAGCGGCTACCGGTGGTAGCATTGAAGGAGTGGATTGAGGGGAGATTTGGGGGGGCAGTGGATTCAAGCAAGCCTGTGGCAACGCAGCTGTGTATGTACTCAATGACACCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGGCAAGGATGTGGTGATCGGCGGCGGGTTCTCGGGGCACGGGTTCAAAATGTCACCGGCGATCGGGAGAGTGCTGGCAGATCTTGCGGTGGACGGGACGGCGGAAAGGGTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGGAATTTCAAGAGCTTTTTAGACCAAGTAAAAGCATCACCACTATAGCTCCTCTGATTCGTTTATGGGTTTCATGCTTATTCTCTCGTCTCTTCCATCGGTAATATGTTCATTTAGTTTTTGAATTTTGGTAATTATGTTTAATAATTTCAATTGCTTTGTTTAGTTTATGCTTCCTACTCATCTAAATTATGGTGTATGCTTCCTACTCATCTAAATTATGGTGTTCTTGCTGATTCAATATGCTTCATACTCATCTAAATTATGGTGTTCTTGCTAATTCAAGAATAGCTCAACAAATAATAAAAAAAAAATTGTATTTCTATTTTTATTTGCTCGGTAAGAATAAGATGTGTGGTTGAAGAG

Coding sequence (CDS)

ATGGAAGATTCCGTTAGTCAATTCGACGTCATCGTCGTCGGCGGCGGCGTAATGGGCAGTTCAACGGCGTACCATCTCGCGAAAAGAGGAAACAGAGTTGTGATTCTGGAGCAGTTCGATTTCCTCCACCACCGAGGGTCTTCCCACGGCGAGTCACGCACCATACGCGCCACCTACCCGGAGGACTACTACCACCGTCTGGTCATGGACTCCTACGACCTCTGGCGGGCCGCCGAGTTGGAAATCGGCTACCGGGTCTATTTTCCGGCGGAGCAGCTCGATTTCGGCCCATCGGACGACAAAAGTCTCGCTGCCGTCATCGACACCAGCCAGAAACTTTCGATTCCCCATCGGGTCCTCGACCACCGGCAACTAGCGGAGAAATACTCCGGCAGGGTGGCGATTCCGGCGGATTGGGTGGCGGTGTGGAGCAAGCACGGCGGCGTAATCAAGCCCACCAAGGCGGTGGCTATGTTCCAAACTTTGGCGTACAAAAACGGAGCCGTTTTGAAGGACAATTCAGAGGTGGTGGCAATAAAGAGGGATGAGGGTAGTGGAGGAATTGTTGTTTCCACAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACGGTTGGAGCTTGGGCTGCAAAGTTAGTTAAATTAGTTGGTGGGATTGAATTGCCAATTCAGCCATTGGAGACTACTGTTTGCTACTGGCGGATCAAGGAGGGGGCCGAGGCCGAGTACGCCATCGGAGGGGATTTCCCGACGTTCGCCAGCTACGGCGACCCGTACATTTACGGGACGCCGTCGCTGGAGTTCCCAGGGCTGATGAAGGTAGCGGTGCACGGCGGGCATCCATGCGATCCCGACAAGCGGCCGTGGAGGGGCGGGGAGCGGCTACCGGTGGTAGCATTGAAGGAGTGGATTGAGGGGAGATTTGGGGGGGCAGTGGATTCAAGCAAGCCTGTGGCAACGCAGCTGTGTATGTACTCAATGACACCAGATGAGGACTTTGTGATTGATTTTTTGGGAGGGGAATTTGGCAAGGATGTGGTGATCGGCGGCGGGTTCTCGGGGCACGGGTTCAAAATGTCACCGGCGATCGGGAGAGTGCTGGCAGATCTTGCGGTGGACGGGACGGCGGAAAGGGTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGGAATTTCAAGAGCTTTTTAGACCAAGTAAAAGCATCACCACTATAG

Protein sequence

MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVVALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVKASPL

Homology

[Close]

BLAST of MC11g0526 vs. ExPASy Swiss-Prot
Match: Q9SJA7 (Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.0e-161
Identity = 266/403 (66.00%), Postives = 328/403 (81.39%), Query Frame = 0


Query: 7   QFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHR 66
           +FDVIVVG GVMGSS AY LAKRG + ++LEQFDFLHHRGSSHGESRTIRATYPEDYY+ 
Sbjct: 8   RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYYYS 67

Query: 67  LVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLA 126
           +V +S  LW AA+ EIGY+V+FP +Q D GP+D +SL +V+ T QK  + HRV+D   ++
Sbjct: 68  MVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMDSHAVS 127

Query: 127 EKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSG 186
           E +SGR++IP +W+ V ++ GG+IKPTKAV+MFQTLA  +GA+L+DN++V  IKRD  SG
Sbjct: 128 EHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKRDGESG 187

Query: 187 -GIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEY 246
            G++V T  G++F GKKC+VT GAW +KLVK V GI+ P++PLETTVCYWRIKEG E ++
Sbjct: 188 EGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEGHEEKF 247

Query: 247 AIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVVALKEW 306
            I G+FPTFASYG PY+YGTPSLE+PGL+KVAVHGG+ CDPDKRPW  G +L    LKEW
Sbjct: 248 TIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKLE--ELKEW 307

Query: 307 IEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSPAI 366
           I+ RFGG VDS  PVATQLCMYSMTPDEDFVIDFLGGEFG+DVV+GGGFSGHGFKM+PA+
Sbjct: 308 IKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAV 367

Query: 367 GRVLADLA--VDGTAERVELKYFRIARFEENPKGNFKSFLDQV 407
           GR+LAD+A  V+     VE+K F + RFE+NPKGN K + DQV
Sbjct: 368 GRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQV 408

[Close]

BLAST of MC11g0526 vs. ExPASy Swiss-Prot
Match: Q29RU9 (Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 8.3e-62
Identity = 138/390 (35.38%), Postives = 207/390 (53.08%), Query Frame = 0


Query: 8   FDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHRL 67
           +D IV+G G+ G  TAYHLAK   +V++LEQF   H RGSSHG+SR IR  YPED+Y ++
Sbjct: 8   YDAIVIGAGIQGCFTAYHLAKHSKKVLLLEQFFLPHSRGSSHGQSRIIRRAYPEDFYTQM 67

Query: 68  VMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLAE 127
           + + Y LW   E E G ++Y     L  G  ++  L  +  T  +  + H+ L   +L +
Sbjct: 68  MAECYSLWAQLEHEAGTQLYRQTGLLLLGMKENPELKIIQATLSRQGVEHQCLSSEELKQ 127

Query: 128 KYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSGG 187
           ++   + +    V +    GGV+   KA+   Q    + G ++ D  +VV IK    SG 
Sbjct: 128 RFP-NIRLARGEVGLLEVSGGVLYADKALRALQDAIRQLGGIVHDGEKVVEIK----SGL 187

Query: 188 IVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEYAI 247
            V+       ++ K  ++T G W  +L++ +G  ELP+Q L   VCYW  +E     Y++
Sbjct: 188 PVMVKTTSRSYQAKSLIITAGPWTNRLLRPLGA-ELPLQTLRINVCYW--QEKVPGSYSV 247

Query: 248 GGDFPTFASYG----DPYIYGTPSLEFPGLMKVAVHGGHPCDPDKR--PWRGGERLPVVA 307
              FP F   G      +IYG PS E+PGLMKV  H G+  DP++R  P    +   V  
Sbjct: 248 SQAFPCFMGLGLSLAPHHIYGLPSREYPGLMKVCYHHGNNADPEERDCPAAFSDIQDVHI 307

Query: 308 LKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKM 367
           L  ++           +P   + CMY+ TPD  FV+D        ++VIG GFSGHGFK+
Sbjct: 308 LSGFVRDHLPDL--QPEPAVMEHCMYTNTPDGHFVLD--RHPKYDNIVIGAGFSGHGFKL 367

Query: 368 SPAIGRVLADLAVDGTAERVELKYFRIARF 392
           SP +G++L +L++  T    +L  FRI+RF
Sbjct: 368 SPVVGKILYELSMKLTPS-YDLTPFRISRF 384

[Close]

BLAST of MC11g0526 vs. ExPASy Swiss-Prot
Match: Q9P0Z9 (Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2)

HSP 1 Score: 236.1 bits (601), Expect = 7.0e-61
Identity = 135/388 (34.79%), Postives = 208/388 (53.61%), Query Frame = 0


Query: 8   FDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHRL 67
           +D IV+G G+ G  TAYHLAK   R+++LEQF   H RGSSHG+SR IR  Y ED+Y R+
Sbjct: 8   WDAIVIGAGIQGCFTAYHLAKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYLEDFYTRM 67

Query: 68  VMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLAE 127
           + + Y +W   E E G +++     L  G  +++ L  +     +  + H+ L   +L +
Sbjct: 68  MHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCLSSEELKQ 127

Query: 128 KYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSGG 187
           ++   + +P   V +    GGVI   KA+   Q    + G +++D  +VV I      G 
Sbjct: 128 RFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI----NPGL 187

Query: 188 IVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEYAI 247
           +V        ++ K  V+T G W  +L++ + GIE+P+Q L   VCYWR  E     Y +
Sbjct: 188 LVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--EMVPGSYGV 247

Query: 248 GGDFPTFASYG--DPYIYGTPSLEFPGLMKVAVHGGHPCDPDKR--PWRGGERLPVVALK 307
              FP F   G    +IYG P+ E+PGLMKV+ H G+  DP++R  P    +   V  L 
Sbjct: 248 SQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTDIGDVQILS 307

Query: 308 EWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSP 367
            ++           +P   + CMY+ TPDE F++D        ++VIG GFSGHGFK++P
Sbjct: 308 SFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFSGHGFKLAP 367

Query: 368 AIGRVLADLAVDGTAERVELKYFRIARF 392
            +G++L +L++  T    +L  FRI+RF
Sbjct: 368 VVGKILYELSMKLTPS-YDLAPFRISRF 382

[Close]

BLAST of MC11g0526 vs. ExPASy Swiss-Prot
Match: P79371 (Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.9e-60
Identity = 133/388 (34.28%), Postives = 206/388 (53.09%), Query Frame = 0


Query: 8   FDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHRL 67
           +D IV+G G+ G  T YHL K   R+++LEQF   H RGSSHG+SR IR  Y ED+Y R+
Sbjct: 8   WDAIVIGAGIQGCFTVYHLVKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYLEDFYTRM 67

Query: 68  VMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLAE 127
           + + Y +W   E E G +++     L  G  +++ L  +     +  + H+ L   +L +
Sbjct: 68  MHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCLSSEELKQ 127

Query: 128 KYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSGG 187
           ++   + +P   V +    GGVI   KA+   Q    + G +++D  +VV I      G 
Sbjct: 128 RFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI----NPGL 187

Query: 188 IVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEYAI 247
           +V        ++ K  V+T G W  +L++ + GIE+P+Q L   VCYWR  E     Y +
Sbjct: 188 LVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--EMVPGSYGV 247

Query: 248 GGDFPTFASYG--DPYIYGTPSLEFPGLMKVAVHGGHPCDPDKR--PWRGGERLPVVALK 307
              FP F   G    +IYG P+ E+PGLMKV+ H G+  DP++R  P    +   V  L 
Sbjct: 248 SQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTDIGDVQILS 307

Query: 308 EWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSP 367
            ++           +P   + CMY+ TPDE F++D        ++VIG GFSGHGFK++P
Sbjct: 308 SFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAGFSGHGFKLAP 367

Query: 368 AIGRVLADLAVDGTAERVELKYFRIARF 392
            +G++L +L++  T    +L  FRI+RF
Sbjct: 368 VVGKILYELSMKLTPS-YDLAPFRISRF 382

[Close]

BLAST of MC11g0526 vs. ExPASy Swiss-Prot
Match: Q9D826 (Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.8e-57
Identity = 134/395 (33.92%), Postives = 201/395 (50.89%), Query Frame = 0


Query: 8   FDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHRL 67
           +D IV+G G+ G  TAYHLAK    V++LEQF   H RGSSHG+SR IR  YPED+Y  +
Sbjct: 8   WDAIVIGAGIQGCFTAYHLAKHSKSVLLLEQFFLPHSRGSSHGQSRIIRKAYPEDFYTMM 67

Query: 68  VMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLAE 127
           + + Y  W   E E G +++   E L  G  ++  L  +  T  +  I H  L    L +
Sbjct: 68  MKECYQTWAQLEREAGTQLHRQTELLLLGTKENPGLKTIQATLSRQGIDHEYLSSVDLKQ 127

Query: 128 KYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSGG 187
           ++   +      V +  K GGV+   KA+   Q +  + G  + D  +VV I+     G 
Sbjct: 128 RFP-NIRFTRGEVGLLDKTGGVLYADKALRALQHIICQLGGTVCDGEKVVEIR----PGL 187

Query: 188 IVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEYAI 247
            V      + ++    V+T G W  +L+  + GIELP+Q L   VCYWR  E     Y +
Sbjct: 188 PVTVKTTLKSYQANSLVITAGPWTNRLLHPL-GIELPLQTLRINVCYWR--EKVPGSYGV 247

Query: 248 GGDFPTF--ASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKR--PWRGGERLPVVALK 307
              FP          +IYG P+ E+PGLMK+  H G   DP++R  P    +   V  L 
Sbjct: 248 SQAFPCILGLDLAPHHIYGLPASEYPGLMKICYHHGDNVDPEERDCPKTFSDIQDVQILC 307

Query: 308 EWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSP 367
            ++     G    ++P   + CMY+ TPDE F++D        ++VIG GFSGHGFK++P
Sbjct: 308 HFVRDHLPGL--RAEPDIMERCMYTNTPDEHFILDC--HPKYDNIVIGAGFSGHGFKLAP 367

Query: 368 AIGRVLADLAVDGTAERVELKYFRIARFEENPKGN 399
            +G++L +L++       +L  FR++RF    K +
Sbjct: 368 VVGKILYELSMK-LPPSYDLAPFRMSRFSTLSKAH 389

[Close]

BLAST of MC11g0526 vs. NCBI nr
Match: XP_022936028.1 (probable sarcosine oxidase [Cucurbita moschata])

HSP 1 Score: 711 bits (1836), Expect = 9.93e-257
Identity = 345/407 (84.77%), Postives = 367/407 (90.17%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA+KNGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           R+  SGG+VVSTANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGGDFPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDPDKR W  G  LP+ 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFEENPKGN KSF DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVR 407

[Close]

BLAST of MC11g0526 vs. NCBI nr
Match: XP_022975268.1 (probable sarcosine oxidase [Cucurbita maxima])

HSP 1 Score: 708 bits (1828), Expect = 1.64e-255
Identity = 343/407 (84.28%), Postives = 367/407 (90.17%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA+KNGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RD  SGG+VVS ANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGG+FPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDPDKR W  G +LP+ 
Sbjct: 241 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALK+WIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFEENPKGN K F DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVR 407

[Close]

BLAST of MC11g0526 vs. NCBI nr
Match: KAG7024517.1 (putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708 bits (1827), Expect = 2.33e-255
Identity = 343/407 (84.28%), Postives = 367/407 (90.17%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LW+ AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWQTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA++NGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFRNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RD  SGG+VVS ANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 RDGSSGGVVVSIANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGGDFPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDP+KR W  G  LP+ 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPNKRSWGSGGELPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFEENPKGN KSF DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVR 407

[Close]

BLAST of MC11g0526 vs. NCBI nr
Match: XP_023534889.1 (probable sarcosine oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706 bits (1822), Expect = 1.35e-254
Identity = 343/407 (84.28%), Postives = 364/407 (89.43%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKY GRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA+KNGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYYGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RD  SGG+VVS ANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 RDGSSGGVVVSIANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGGDFPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDPDKR W  G  LP+ 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFE NPKGN K F DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGMELKYFRIARFEGNPKGNVKDFADQVR 407

[Close]

BLAST of MC11g0526 vs. NCBI nr
Match: XP_038896765.1 (probable sarcosine oxidase [Benincasa hispida])

HSP 1 Score: 701 bits (1809), Expect = 1.29e-252
Identity = 342/407 (84.03%), Postives = 364/407 (89.43%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +QFDVIVVG GVMGSST YHLAK GNRV+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQFDVIVVGAGVMGSSTGYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD GPSDDKSLAAV+DT +K SIPH VL
Sbjct: 61  EDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVDTCRKHSIPHMVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IP+DWVAVWSK+GGVIKPTKAV+MFQ LAYKNGAVLKDN+EVV IK
Sbjct: 121 DRGQLAEKYSGRVEIPSDWVAVWSKYGGVIKPTKAVSMFQCLAYKNGAVLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RDE +G IVVS ANGE F GKKCVVTVGAWA KLVK V  IELPIQPLE TV YWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFSGKKCVVTVGAWARKLVKSVSRIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AEAEYAIGGDFPTFASYG+PY+YGTPSLEFPGL+KVA+HGGH C+PDKR W  G RLP+ 
Sbjct: 241 AEAEYAIGGDFPTFASYGNPYVYGTPSLEFPGLIKVAMHGGHQCNPDKRSWGSGGRLPIS 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWIE RFGG VDSS P+ATQLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEERFGGRVDSSDPIATQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LA+LA+ G AE VELKYF+IARFEENPKGN KSF DQVK
Sbjct: 361 MSPAIGRILAELALKGEAEGVELKYFKIARFEENPKGNIKSFADQVK 407

[Close]

BLAST of MC11g0526 vs. ExPASy TrEMBL
Match: A0A6J1FC48 (Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1)

HSP 1 Score: 711 bits (1836), Expect = 4.81e-257
Identity = 345/407 (84.77%), Postives = 367/407 (90.17%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA+KNGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           R+  SGG+VVSTANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGGDFPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDPDKR W  G  LP+ 
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFEENPKGN KSF DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVR 407

[Close]

BLAST of MC11g0526 vs. ExPASy TrEMBL
Match: A0A6J1IIQ6 (Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 7.95e-256
Identity = 343/407 (84.28%), Postives = 367/407 (90.17%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M DS +Q+DVIVVG GVMGSSTAYHLAK GN+V+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIGYRVYFPAEQLD G SDDKSLAA +DT +K SIPH VL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  QLAEKYSGRV IPADWVAVWSK+GGVIKPTKAV+MFQTLA+KNGA LKDN+EVV IK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RD  SGG+VVS ANGERFRGKKCVVTVGAWA KLVK VGGIELPIQPLE TV YWRIKEG
Sbjct: 181 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
           AE EYAIGG+FPTFASYGD YIYGTPSLEFPGL+KVAVHGGH CDPDKR W  G +LP+ 
Sbjct: 241 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALK+WIEGRFGG VDSS+PV+TQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LADLA+ G AE +ELKYFRIARFEENPKGN K F DQV+
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVR 407

[Close]

BLAST of MC11g0526 vs. ExPASy TrEMBL
Match: A0A0A0LJG6 (Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1)

HSP 1 Score: 670 bits (1728), Expect = 1.35e-240
Identity = 324/407 (79.61%), Postives = 356/407 (87.47%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S + FDVIVVG GVMGSSTAYHLAK GNRV+ILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYY+ LVM+SY+LWR AE EIGY+VYFP EQLD G  DDKSL AV+DT +K SIPH VL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  +L EKYSGRV IPADWV VWSK+GGVIKPTKAV+M+QTLAYKNGAV+KDN+EVV IK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RDE +G IVVS ANGE FRGKKCVVTVGAW+ KLVK VGGIELPI+PLE +V YWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
            EAEYAIGG FPT ASYG+PY+YGTPSLEFPGL+KVA+HGGH C+PDKR W  G RLP+ 
Sbjct: 241 FEAEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPIA 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWI+ +FGG VDSS PV+TQ CMYSMTPD DFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSP IGR+LA+LA+DG AE VELKYF++ARFEENPKGN KSF DQVK
Sbjct: 361 MSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVK 407

[Close]

BLAST of MC11g0526 vs. ExPASy TrEMBL
Match: A0A5D3E5F8 (Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006680 PE=3 SV=1)

HSP 1 Score: 660 bits (1702), Expect = 1.37e-236
Identity = 320/407 (78.62%), Postives = 356/407 (87.47%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S +QFDVIVVG GVMGSSTAYHLAK GNRV++LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIG++VY+PAEQLD GPS+ +SL AV+DT  K SIPH VL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  +L EKYSGRV IPA+WVAV SK+GGVIKPTKAV+MFQTLAYKNG VLKDN+EVV IK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RDE +G IVVSTANGE F GKKCVVTVGAW+ KLVK VGGIELPI PLE +V YWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
            EAEYAI G FPT ASYG+PY+YGTPSLEFPGL+KVA+H G+ C+PDKR W    RLP+ 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGRDGRLPIA 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWI+ +FGG VDSS+PV++QLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LA+LA+ G AE VELKYF++ARFEENPKGN KSF DQVK
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVK 407

[Close]

BLAST of MC11g0526 vs. ExPASy TrEMBL
Match: A0A1S3CR44 (Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 1.95e-236
Identity = 320/407 (78.62%), Postives = 356/407 (87.47%), Query Frame = 0


Query: 1   MEDSVSQFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYP 60
           M  S +QFDVIVVG GVMGSSTAYHLAK GNRV++LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 61  EDYYHRLVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVL 120
           EDYYH LVM+SY+LWR AE EIG++VY+PAEQLD GPS+ +SL AV+DT  K SIPH VL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 121 DHRQLAEKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIK 180
           D  +L EKYSGRV IPA+WVAV SK+GGVIKPTKAV+MFQTLAYKNG VLKDN+EVV IK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 181 RDEGSGGIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEG 240
           RDE +G IVVSTANGE F GKKCVVTVGAW+ KLVK VGGIELPI PLE +V YWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 241 AEAEYAIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVV 300
            EAEYAI G FPT ASYG+PY+YGTPSLEFPGL+KVA+H G+ C+PDKR W    RLP+ 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGREGRLPIA 300

Query: 301 ALKEWIEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360
           ALKEWI+ +FGG VDSS+PV++QLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 361 MSPAIGRVLADLAVDGTAERVELKYFRIARFEENPKGNFKSFLDQVK 407
           MSPAIGR+LA+LA+ G AE VELKYF++ARFEENPKGN KSF DQVK
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVK 407

[Close]

BLAST of MC11g0526 vs. TAIR 10
Match: AT2G24580.1 (FAD-dependent oxidoreductase family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 1.5e-162
Identity = 266/403 (66.00%), Postives = 328/403 (81.39%), Query Frame = 0


Query: 7   QFDVIVVGGGVMGSSTAYHLAKRGNRVVILEQFDFLHHRGSSHGESRTIRATYPEDYYHR 66
           +FDVIVVG GVMGSS AY LAKRG + ++LEQFDFLHHRGSSHGESRTIRATYPEDYY+ 
Sbjct: 8   RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYYYS 67

Query: 67  LVMDSYDLWRAAELEIGYRVYFPAEQLDFGPSDDKSLAAVIDTSQKLSIPHRVLDHRQLA 126
           +V +S  LW AA+ EIGY+V+FP +Q D GP+D +SL +V+ T QK  + HRV+D   ++
Sbjct: 68  MVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRVMDSHAVS 127

Query: 127 EKYSGRVAIPADWVAVWSKHGGVIKPTKAVAMFQTLAYKNGAVLKDNSEVVAIKRDEGSG 186
           E +SGR++IP +W+ V ++ GG+IKPTKAV+MFQTLA  +GA+L+DN++V  IKRD  SG
Sbjct: 128 EHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANIKRDGESG 187

Query: 187 -GIVVSTANGERFRGKKCVVTVGAWAAKLVKLVGGIELPIQPLETTVCYWRIKEGAEAEY 246
            G++V T  G++F GKKC+VT GAW +KLVK V GI+ P++PLETTVCYWRIKEG E ++
Sbjct: 188 EGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIKEGHEEKF 247

Query: 247 AIGGDFPTFASYGDPYIYGTPSLEFPGLMKVAVHGGHPCDPDKRPWRGGERLPVVALKEW 306
            I G+FPTFASYG PY+YGTPSLE+PGL+KVAVHGG+ CDPDKRPW  G +L    LKEW
Sbjct: 248 TIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPGVKLE--ELKEW 307

Query: 307 IEGRFGGAVDSSKPVATQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSPAI 366
           I+ RFGG VDS  PVATQLCMYSMTPDEDFVIDFLGGEFG+DVV+GGGFSGHGFKM+PA+
Sbjct: 308 IKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHGFKMAPAV 367

Query: 367 GRVLADLA--VDGTAERVELKYFRIARFEENPKGNFKSFLDQV 407
           GR+LAD+A  V+     VE+K F + RFE+NPKGN K + DQV
Sbjct: 368 GRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQV 408

The following BLAST results are available for this feature:

BLAST of MC11g0526 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJA7	2.0e-161	66.00	Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=...	[more]
Q29RU9	8.3e-62	35.38	Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2	[more]
Q9P0Z9	7.0e-61	34.79	Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2	[more]
P79371	5.9e-60	34.28	Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=...	[more]
Q9D826	2.8e-57	33.92	Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1	[more]

BLAST of MC11g0526 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022936028.1	9.93e-257	84.77	probable sarcosine oxidase [Cucurbita moschata]	[more]
XP_022975268.1	1.64e-255	84.28	probable sarcosine oxidase [Cucurbita maxima]	[more]
KAG7024517.1	2.33e-255	84.28	putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023534889.1	1.35e-254	84.28	probable sarcosine oxidase [Cucurbita pepo subsp. pepo]	[more]
XP_038896765.1	1.29e-252	84.03	probable sarcosine oxidase [Benincasa hispida]	[more]

BLAST of MC11g0526 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FC48	4.81e-257	84.77	Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1	[more]
A0A6J1IIQ6	7.95e-256	84.28	Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1	[more]
A0A0A0LJG6	1.35e-240	79.61	Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1	[more]
A0A5D3E5F8	1.37e-236	78.62	Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CR44	1.95e-236	78.62	Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1	[more]

BLAST of MC11g0526 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24580.1	1.5e-162	66.00	FAD-dependent oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006281	Sarcosine oxidase, monomeric	TIGRFAM	TIGR01377	TIGR01377	coord: 8..397 e-value: 1.8E-150 score: 498.9
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 9..378 e-value: 8.9E-106 score: 356.1
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 7..392
IPR006076	FAD dependent oxidoreductase	PFAM	PF01266	DAO	coord: 9..372 e-value: 9.6E-49 score: 166.8
None	No IPR available	GENE3D	3.30.9.10		coord: 63..331 e-value: 8.9E-106 score: 356.1
None	No IPR available	PANTHER	PTHR10961	PEROXISOMAL SARCOSINE OXIDASE	coord: 5..401
None	No IPR available	PANTHER	PTHR10961:SF7	PEROXISOMAL SARCOSINE OXIDASE	coord: 5..401
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 227..331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0526.1	MC11g0526.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046653	tetrahydrofolate metabolic process
molecular_function	GO:0008115	sarcosine oxidase activity
molecular_function	GO:0016491	oxidoreductase activity