MC10g1145 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g1145 vs. ExPASy Swiss-Prot
Match: Q9FIZ7 (5-oxoprolinase OS=Arabidopsis thaliana OX=3702 GN=OXP1 PE=1 SV=1)

HSP 1 Score: 2124.7 bits (5504), Expect = 0.0e+00
Identity = 1038/1266 (81.99%), Postives = 1145/1266 (90.44%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MG+  E KLRFCIDRGGTFTDVYAEIPG  DG V+KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGTVIEGKLRFCIDRGGTFTDVYAEIPGHSDGHVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKI RTSKIPT+ I+WIRMGTTVATNALLERKGERIALCVTKGF+DLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTDKIQWIRMGTTVATNALLERKGERIALCVTKGFKDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTV+KPSNLYE+VIEVDERV L     + + D   S +KGVSG  +RVVK  + E LK
Sbjct: 121  FDLTVAKPSNLYEEVIEVDERVVLAL---EDDDDDEGSLIKGVSGEFLRVVKPFDGEGLK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+ LL +GISCLAVVL+HSYTYP+HE+ +EKLA+ +GF+HVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKGLLDKGISCLAVVLMHSYTYPKHEMDVEKLALEMGFRHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TA+VDAYLTPVIKEYLSGF+SKFD+  GKVNVLFMQSDGGLA E+RFSGHKAVLSGPAGG
Sbjct: 241  TATVDAYLTPVIKEYLSGFISKFDDDLGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF LET KPLIGFDMGGTSTDVSRY GSYEQV+ETQIAG IIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFGLETEKPLIGFDMGGTSTDVSRYDGSYEQVIETQIAGTIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGP+SVGAHPGPVCYRKGGELAVTDANLVLGFVIPD+FPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPDSVGAHPGPVCYRKGGELAVTDANLVLGFVIPDYFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLD+ ATR  FEKLA +IN YRK+ DPS+K M++EEIA+GFV+VANETMCRPIRQLT
Sbjct: 421  EDQPLDVAATREAFEKLAGQINIYRKSQDPSAKDMSVEEIAMGFVSVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIAR LGMKE+ VHR+CGILSAYGMGLADV+E+ QE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARSLGMKEVLVHRYCGILSAYGMGLADVIEDAQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVY  ES+ EV RRE  LL++V+ KL  QGF +G+I TETYLNLRY+GTDTAIMVKG
Sbjct: 541  PYSAVYGPESLSEVFRRETVLLREVREKLQEQGFGDGNISTETYLNLRYDGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            +K  DG  FD+AAEF+KLF+QEYGF+LQNRN+LICD+RVRGIG+T++LKPRA E     P
Sbjct: 601  KKTGDGSAFDYAAEFLKLFEQEYGFKLQNRNLLICDVRVRGIGVTSILKPRAVEAAPVTP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            K+E H KVYF  GW +TPLFKL+NLG G+ ILGPAIIMNGNSTVIVEP CKA ITKYGNI
Sbjct: 661  KVERHYKVYFEGGWHDTPLFKLENLGFGHEILGPAIIMNGNSTVIVEPQCKAIITKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIE++    + K++E V DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEVEPATSSVKLAENVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            F PDGGLVANAPHVPVHLGAMSSTVRWQ++ WG+NLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FSPDGGLVANAPHVPVHLGAMSSTVRWQLKHWGENLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFD  KL+FFVASRGHHAE+GGITPGSMPPFSK IWEEGAAIKAFK+VEKG+FQEEGI
Sbjct: 841  TPVFDKGKLVFFVASRGHHAEVGGITPGSMPPFSKAIWEEGAAIKAFKVVEKGVFQEEGI 900

Query: 901  IKLLQFPSSDEGV--VPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMT 960
            +KLLQFPSSDE    +PGTRR+QDNLSDL AQ+AAN RGISLIKELI QYGL TVQAYM 
Sbjct: 901  VKLLQFPSSDETTTKIPGTRRIQDNLSDLQAQIAANQRGISLIKELIEQYGLGTVQAYMK 960

Query: 961  YVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGE 1020
            YVQLNAEEAVREMLKSVA+RVSS +  S  G+ +TIEE+DYMDDGS+IHLKLTID  +GE
Sbjct: 961  YVQLNAEEAVREMLKSVANRVSSETPNSRVGNSVTIEEEDYMDDGSIIHLKLTIDADKGE 1020

Query: 1021 ANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLS 1080
            A+FDF+GTSPEVYGNWNAPEAVT+AAVIYCLRC+V+VDIPLNQGCLAPV+I IP+GS LS
Sbjct: 1021 ASFDFTGTSPEVYGNWNAPEAVTSAAVIYCLRCLVNVDIPLNQGCLAPVEIRIPAGSFLS 1080

Query: 1081 PSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGP 1140
            PSEKAA+VGGNVLTSQR+TDV+LTAFQACACSQGCMNNLTFGDDTFGYYETIGGG GAGP
Sbjct: 1081 PSEKAAVVGGNVLTSQRVTDVVLTAFQACACSQGCMNNLTFGDDTFGYYETIGGGCGAGP 1140

Query: 1141 CWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQ 1200
             W+GTSGVQCHMTNTRMTDPEIFEQRYPVLLH F LRENSGGNG++KGGDGLVREIEF++
Sbjct: 1141 TWNGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFGLRENSGGNGLHKGGDGLVREIEFRK 1200

Query: 1201 PVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTP 1260
            PVVVSILSERRVH+PRGL GG++G RGAN+L+ KDKRR+YLGGKN+V V AGEILQILTP
Sbjct: 1201 PVVVSILSERRVHSPRGLNGGQNGLRGANYLITKDKRRIYLGGKNTVHVEAGEILQILTP 1260

Query: 1261 GGGGWG 1265
            GGGG+G
Sbjct: 1261 GGGGFG 1263

BLAST of MC10g1145 vs. ExPASy Swiss-Prot
Match: Q8K010 (5-oxoprolinase OS=Mus musculus OX=10090 GN=Oplah PE=1 SV=1)

HSP 1 Score: 1416.4 bits (3665), Expect = 0.0e+00
Identity = 722/1272 (56.76%), Postives = 907/1272 (71.31%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  E++  F IDRGGTFTDV+A+ PG    +V+KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGSP-EERFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  + R   + T +I  IRMGTTVATNALLER+GER+AL VT+GFRDLL IG QARPD+
Sbjct: 61   RGVLLPRGRPLDTSHIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDL 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDL V  P  LYE+V+EVDERV L  G+         S VKG +G L+ + + ++  AL+
Sbjct: 121  FDLAVPMPEVLYEEVVEVDERVLLYRGEPGAG-----SPVKGCTGDLLEIQQPVDLAALR 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              LE LL RGI  LAVVL+HSYT+ QHE  +  LA  LGF HVSLSS + PMVR VPRG 
Sbjct: 181  GKLEGLLTRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGH 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TA  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLA  + FSG +AVLSGPAGG
Sbjct: 241  TACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYS T ++LE  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVA
Sbjct: 301  VVGYSTTTYQLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP 
Sbjct: 361  AGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPG 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPL  EA+R   E +A E+NS+  +    +  +++EE+A+GFV VANE MCRPIR LT
Sbjct: 421  EDQPLSPEASRKALEAVAMEVNSFLASGPCPASQLSLEEVAMGFVRVANEAMCRPIRALT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            + +GH+   H LACFGGAG QHACAIAR LGM  + +HR  G+LSA G+ LADVV E QE
Sbjct: 481  QARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            P S  Y  E+  ++ +R + L +Q    L  QGF    I TE++L+LRY+GTD A+MV  
Sbjct: 541  PCSLSYTPETFAQLDQRLSRLEEQCVDALQAQGFSRSQISTESFLHLRYQGTDCALMVSA 600

Query: 601  QKIDDGV----GFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGR 660
             +           DF A FV+ + +E+GF +  R++++ D+RVRG G + +      + +
Sbjct: 601  NQHPATTCSPRAGDFGAAFVERYMREFGFIIPERSVVVDDVRVRGTGRSGLQLEETSKIQ 660

Query: 661  SSDPKIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITK 720
            S  P +E   + YF  G++ TP++ L  LG G+ + GP +I++ NST++VEP C+A + +
Sbjct: 661  SGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVIE 720

Query: 721  YGNIKIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDF 780
             G+I+I + +      + +  +D +QLSIF+HRFM IAEQMGR LQRT+ISTNIKERLDF
Sbjct: 721  TGDIRISVGAE--APSMIDTKLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDF 780

Query: 781  SCALFGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPD 840
            SCALFGPDGGLV+NAPH+PVHLGAM  TV++QI+  G +L+ GDVL++NHP AGGSHLPD
Sbjct: 781  SCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPD 840

Query: 841  ITVITPVF--DNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGI 900
            +TVITPVF     + +F+VASRGHHA+IGGITPGSMPP S  + +EGA   +FKLV+ G+
Sbjct: 841  LTVITPVFWPGQSRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGV 900

Query: 901  FQEEGIIKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQ 960
            FQEE + + LQ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL  VQ
Sbjct: 901  FQEEAVTEALQAPGKISG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQ 960

Query: 961  AYMTYVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDP 1020
            AYM ++Q NAE AVR+ML++        ++    G P+ +   D+MDDGS I L + I+ 
Sbjct: 961  AYMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSAKDHMDDGSPICLHVQINL 1020

Query: 1021 QRGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSG 1080
             +G A FDFSG+  EV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP G
Sbjct: 1021 NQGSAVFDFSGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVQVIIPKG 1080

Query: 1081 SLLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGS 1140
            S+L PS +AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+ GG+
Sbjct: 1081 SILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNARMGYYETVAGGA 1140

Query: 1141 GAGPCWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREI 1200
            GAGP WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+VRE+
Sbjct: 1141 GAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVREL 1200

Query: 1201 EFKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQ 1260
             F++  ++S+L+ERR   P GL GG+ G RG N L++KD R V LGGK SVTV  G+   
Sbjct: 1201 VFREEALLSVLTERRAFQPYGLHGGEPGTRGLNLLIRKDGRTVNLGGKTSVTVYPGDAFC 1256

Query: 1261 ILTPGGGGWGCP 1267
            + TPGGGG+G P
Sbjct: 1261 LHTPGGGGYGDP 1256

BLAST of MC10g1145 vs. ExPASy Swiss-Prot
Match: O14841 (5-oxoprolinase OS=Homo sapiens OX=9606 GN=OPLAH PE=1 SV=3)

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 727/1276 (56.97%), Postives = 910/1276 (71.32%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  E +  F IDRGGTFTDV+A+ PG    +V+KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGSP-EGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  + R   + + +I  IRMGTTVATNALLERKGER+AL VT+GFRDLL IG QAR D+
Sbjct: 61   AGMLLPRDQPLDSSHIASIRMGTTVATNALLERKGERVALLVTRGFRDLLHIGTQARGDL 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGK-GDGNQDSYTSYVKGVSGGLIRVVKTLNEEAL 180
            FDL V  P  LYE+V+EVDERV L  G+ G G        VKG +G L+ V + ++  AL
Sbjct: 121  FDLAVPMPEVLYEEVLEVDERVVLHRGEAGTGTP------VKGRTGDLLEVQQPVDLGAL 180

Query: 181  KSSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRG 240
            +  LE LL RGI  LAVVL+HSYT+ QHE  +  LA  LGF HVSLSS   PMVR VPRG
Sbjct: 181  RGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGVLARELGFTHVSLSSEAMPMVRIVPRG 240

Query: 241  LTASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAG 300
             TA  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLA  + FSG  AVLSGPAG
Sbjct: 241  HTACADAYLTPAIQRYVQGFCRGFQGQLKDVQVLFMRSDGGLAPMDTFSGSSAVLSGPAG 300

Query: 301  GVVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTV 360
            GVVGYS T ++ E  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTV
Sbjct: 301  GVVGYSATTYQQEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTV 360

Query: 361  AAGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 420
            AAGGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP
Sbjct: 361  AAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGP 420

Query: 421  EEDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQL 480
             E+QPL  EA+R   E +A+E+NS+  N    + P+++EE+A+GFV VANE MCRPIR L
Sbjct: 421  GENQPLSPEASRKALEAVATEVNSFLTNGPCPASPLSLEEVAMGFVRVANEAMCRPIRAL 480

Query: 481  TEMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQ 540
            T+ +GH+   H LACFGGAG QHACAIAR LGM  + +HR  G+LSA G+ LADVV E Q
Sbjct: 481  TQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQ 540

Query: 541  EPYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVK 600
            EP S +Y  E+  ++ +R + L +Q    L  QGF    I TE++L+LRY+GTD A+MV 
Sbjct: 541  EPCSLLYAPETFVQLDQRLSRLEEQCVDALQAQGFPRSQISTESFLHLRYQGTDCALMVS 600

Query: 601  GQK----IDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEG 660
              +           DF A FV+ + +E+GF +  R +++ D+RVRG G + +    A + 
Sbjct: 601  AHQHPATARSPRAGDFGAAFVERYMREFGFVIPERPVVVDDVRVRGTGRSGLRLEDAPKA 660

Query: 661  RSSDPKIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATIT 720
            ++  P+++   + YF  G++ TP++ L  LG G+ + GP +I++ NST++VEP C+A +T
Sbjct: 661  QTGPPRVDKMTQCYFEGGYQETPVYLLAELGYGHKLHGPCLIIDSNSTILVEPGCQAEVT 720

Query: 721  KYGNIKIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLD 780
            K G+I I + +           +D +QLSIF+HRFM IAEQMGR LQRT+ISTNIKERLD
Sbjct: 721  KTGDICISVGAE--VPGTVGPQLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLD 780

Query: 781  FSCALFGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLP 840
            FSCALFGPDGGLV+NAPH+PVHLGAM  TV++QI+  G +L+ GDVL++NHP AGGSHLP
Sbjct: 781  FSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLP 840

Query: 841  DITVITPVF--DNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKG 900
            D+TVITPVF     + +F+VASRGHHA+IGGITPGSMPP S  + +EGA   +FKLV+ G
Sbjct: 841  DLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTMLQQEGAVFLSFKLVQGG 900

Query: 901  IFQEEGIIKLLQFPSSDEGVVP---GTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGL 960
            +FQEE + + L+ P    G VP   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL
Sbjct: 901  VFQEEAVTEALRAP----GKVPNCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGL 960

Query: 961  STVQAYMTYVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKL 1020
              VQAYM ++Q NAE AVR+ML++        ++    G P+ +  +D+MDDGS I L++
Sbjct: 961  DVVQAYMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSSEDHMDDGSPIRLRV 1020

Query: 1021 TIDPQRGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIY 1080
             I   +G A FDFSGT PEV+GN NAP AVT +A+IYCLRC+V  DIPLNQGCLAPV++ 
Sbjct: 1021 QISLSQGSAVFDFSGTGPEVFGNLNAPRAVTLSALIYCLRCLVGRDIPLNQGCLAPVRVV 1080

Query: 1081 IPSGSLLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETI 1140
            IP GS+L PS +AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+
Sbjct: 1081 IPRGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETV 1140

Query: 1141 GGGSGAGPCWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGL 1200
             GG+GAGP WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+
Sbjct: 1141 AGGAGAGPSWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRRGSGGRGRFRGGDGV 1200

Query: 1201 VREIEFKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAG 1260
             RE+ F++  ++S+L+ERR   P GL GG+ GARG N L++K+ R V LGGK SVTV  G
Sbjct: 1201 TRELLFREEALLSVLTERRAFRPYGLHGGEPGARGLNLLIRKNGRTVNLGGKTSVTVYPG 1256

Query: 1261 EILQILTPGGGGWGCP 1267
            ++  + TPGGGG+G P
Sbjct: 1261 DVFCLHTPGGGGYGDP 1256

BLAST of MC10g1145 vs. ExPASy Swiss-Prot
Match: P97608 (5-oxoprolinase OS=Rattus norvegicus OX=10116 GN=Oplah PE=1 SV=2)

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 722/1272 (56.76%), Postives = 909/1272 (71.46%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  E +  F IDRGGTFTDV+A+ PG    +V+KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGSP-EGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  + R   + T  I  IRMGTTVATNALLER+GER+AL VT+GFRDLL IG QARPD+
Sbjct: 61   EGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDL 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDL V  P  LYE+V+EVDERV L  G+         S VKG +G L+ + + ++ EAL+
Sbjct: 121  FDLAVPMPEVLYEEVLEVDERVVLYRGEPGAG-----SPVKGRTGDLLEIQQPVDLEALR 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              LE LL RGI  LAVVL+HSYT+ QHE  +  LA  LGF HVSLSS + PMVR VPRG 
Sbjct: 181  GKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGH 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TA  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLA  + FSG +AVLSGPAGG
Sbjct: 241  TACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYS T + LE  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVA
Sbjct: 301  VVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP 
Sbjct: 361  AGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPG 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPL  EA+R   E +A E+NS+  N    +  +++EE+A+GFV VANE MCRPIR LT
Sbjct: 421  EDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            + +GH+   H LACFGGAG QHACAIAR LGM  + +HR  G+LSA G+ LADVV E QE
Sbjct: 481  QARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            P S  Y  E+  ++ +R + L +Q    L  QGF    I TE++L+LRY+GTD A+MV  
Sbjct: 541  PCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSA 600

Query: 601  QKIDDGV----GFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGR 660
             +           DF A FV+ + +E+GF +  R +++ D+RVRG G + +      + +
Sbjct: 601  HQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQ 660

Query: 661  SSDPKIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITK 720
            +  P +E   + YF  G++ TP++ L  LG G+ + GP +I++ NST++VEP C+A +T 
Sbjct: 661  TGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTD 720

Query: 721  YGNIKIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDF 780
             G+I+I + +      +++  +D +QLSIF+HRFM IAEQMGR LQRT+ISTNIKERLDF
Sbjct: 721  TGDIRISVGAE--GPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDF 780

Query: 781  SCALFGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPD 840
            SCALFGPDGGLV+NAPH+PVHLGAM  TV++QI+  G +L+ GDVL++NHP AGGSHLPD
Sbjct: 781  SCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPD 840

Query: 841  ITVITPVF--DNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGI 900
            +TVITPVF     + +F+VASRGHHA+IGGITPGSMPP S  + +EGA   +FKLV+ G+
Sbjct: 841  LTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGV 900

Query: 901  FQEEGIIKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQ 960
            FQEE + + L+ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL  VQ
Sbjct: 901  FQEEAVTEALRAPGKISG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQ 960

Query: 961  AYMTYVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDP 1020
            AYM ++Q NAE AVR+ML++        ++    G P+ +  +D+MDDGS I L++ I+ 
Sbjct: 961  AYMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSAEDHMDDGSPICLRVQINL 1020

Query: 1021 QRGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSG 1080
             +G A FDF+G+  EV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP G
Sbjct: 1021 SQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKG 1080

Query: 1081 SLLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGS 1140
            S+L PS +AA+VGGNVLTSQR+ DVIL AF AC+ SQGCMNN+T G+   GYYET+ GG+
Sbjct: 1081 SILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGA 1140

Query: 1141 GAGPCWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREI 1200
            GAGP WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+VRE+
Sbjct: 1141 GAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVREL 1200

Query: 1201 EFKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQ 1260
             F++  ++S+L+ERR   P GL GG+ GARG N L++KD R V LGGK SVTV  G++  
Sbjct: 1201 VFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFC 1256

Query: 1261 ILTPGGGGWGCP 1267
            + TPGGGG+G P
Sbjct: 1261 LHTPGGGGYGDP 1256

BLAST of MC10g1145 vs. ExPASy Swiss-Prot
Match: Q75WB5 (5-oxoprolinase OS=Bos taurus OX=9913 GN=OPLAH PE=1 SV=1)

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 715/1268 (56.39%), Postives = 899/1268 (70.90%), Query Frame = 0

Query: 6    EDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEYTGKKI 65
            E +  F IDRGGTFTDV+A+ PG    +V+KLLS DP+NY DAP EGIRRILE+  G  +
Sbjct: 5    EGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYVDAPTEGIRRILEQEGGVLL 64

Query: 66   SRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDIFDLTV 125
             R   + T  I  IRMGTTVATNALLE++GER+AL VT+GFRDLL +  QAR  +FDL V
Sbjct: 65   PRDRPLDTSRIASIRMGTTVATNALLEQQGERVALLVTRGFRDLLHVCTQARAXLFDLAV 124

Query: 126  SKPSNLYEDVIEVDERVELVHG-KGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALKSSLE 185
              P  LYE+V+EVDERV L  G  G G        VKG +G L+ V + ++   L+  LE
Sbjct: 125  PMPETLYEEVLEVDERVVLYRGXPGAGTP------VKGCTGDLLEVQQPVDLGGLRWKLE 184

Query: 186  DLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGLTASV 245
             LL RGI  LAVVL+HSYT+ QHE  +  LA  LGF HVSLSS   PMVR VPRG TA  
Sbjct: 185  GLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACA 244

Query: 246  DAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGGVVGY 305
            DAYLTP I+ Y+ GF   F      V VLFM+SDGGLA  + FSG +AVLSGPAGGVVGY
Sbjct: 245  DAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGY 304

Query: 306  SQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAAGGG 365
            S T + +E  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVAAGGG
Sbjct: 305  SATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGG 364

Query: 366  SKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPEEDQP 425
            S+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP EDQP
Sbjct: 365  SRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQP 424

Query: 426  LDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLTEMKG 485
            L  EA+R   E +A+E+NS+  N    + P+++EE+A+GFV VANE MCRPIR LT+ +G
Sbjct: 425  LSPEASRKALEAVATEVNSFLTNGPCPASPLSLEEVAMGFVRVANEAMCRPIRALTQARG 484

Query: 486  HETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQEPYSA 545
            H+   H LACFGGAG QHACAIAR LGM  + +HR  G+LSA G+ LADVV E QEP S 
Sbjct: 485  HDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSL 544

Query: 546  VYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKGQKID 605
             Y  E+  ++ +R   L +Q    L  QGF    I TE++L+LRY+GTD A+MV   +  
Sbjct: 545  PYAPETFAQLDQRLGRLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHP 604

Query: 606  DGV----GFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 665
                     DF A FV+ + +E+GF +  R +++ D+RVRG G +++      +  S  P
Sbjct: 605  ASARSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGSSSLRLEDVPKAHSGPP 664

Query: 666  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 725
            +++   + YF  G++ TP++ L  LGCG+ + GP +I++ NST++VEP C+A +T+ G+I
Sbjct: 665  RVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDI 724

Query: 726  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 785
            +I + +   T  +    +D + L+IF+HRFM IAEQMGR LQRT+ISTNIKERLDFSCAL
Sbjct: 725  RISVGAE--TASVVGTQLDPIHLTIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCAL 784

Query: 786  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 845
            FGPDGGLV+N PH+PVHLGAM  TV++QI+  G +L+ GDVL++NHP AGGSHLPD+TVI
Sbjct: 785  FGPDGGLVSNVPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVI 844

Query: 846  TPVF--DNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEE 905
            TPVF     + +F+VASRGHHA+IGGITPGSMPP S  + +EGA   +FKLV  G+FQEE
Sbjct: 845  TPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVHGGVFQEE 904

Query: 906  GIIKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMT 965
             + + L+ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL  VQAYM 
Sbjct: 905  AVTEALRAPGKIPG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMG 964

Query: 966  YVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGE 1025
            ++Q NAE AVR+ML++        +A    G P+ +  +D+MDDGS I L++ I+  +G 
Sbjct: 965  HIQANAELAVRDMLRAF------GTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGS 1024

Query: 1026 ANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLS 1085
            A FDFSG+ PEV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP GS+L 
Sbjct: 1025 AVFDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILD 1084

Query: 1086 PSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGP 1145
            PS  AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+ GG+GAGP
Sbjct: 1085 PSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGP 1144

Query: 1146 CWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQ 1205
             WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG++RE+ F++
Sbjct: 1145 GWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFRE 1204

Query: 1206 PVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTP 1265
              ++S+L+ERR   P GL GG+ GARG N L++KD R V LGGK SV V  G++  + TP
Sbjct: 1205 EALLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTP 1256

Query: 1266 GGGGWGCP 1267
            GGGG+G P
Sbjct: 1265 GGGGYGDP 1256

BLAST of MC10g1145 vs. NCBI nr
Match: XP_022143475.1 (5-oxoprolinase [Momordica charantia] >XP_022143476.1 5-oxoprolinase [Momordica charantia])

HSP 1 Score: 2540 bits (6583), Expect = 0.0
Identity = 1266/1266 (100.00%), Postives = 1266/1266 (100.00%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
            SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE
Sbjct: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG
Sbjct: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP
Sbjct: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI
Sbjct: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV
Sbjct: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN
Sbjct: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. NCBI nr
Match: XP_023526770.1 (5-oxoprolinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2384 bits (6178), Expect = 0.0
Identity = 1183/1266 (93.44%), Postives = 1225/1266 (96.76%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS +EDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSSEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT+NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKG+SG LI +VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGISGELIHIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLAWSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGT+TAIMV+ 
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTNTAIMVRS 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK D+GVGFDFAA F KLFQQEYGF+LQNRNILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 601  QKTDNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH KVYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIVEPSCKATITK+GNI
Sbjct: 661  KIEGHYKVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS FCTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KVEIDSAFCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQIE+WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNSAGS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. NCBI nr
Match: XP_022980191.1 (5-oxoprolinase [Cucurbita maxima])

HSP 1 Score: 2383 bits (6175), Expect = 0.0
Identity = 1184/1266 (93.52%), Postives = 1223/1266 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS NEDKLRFCIDRGGTFTDVYAEIPG PDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGLPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT+NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKGVSG LI +VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGTDTAIMV+ 
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK+DDGVGFDFAA F KLFQQEYGF+LQN+NILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 601  QKVDDGVGFDFAAVFEKLFQQEYGFKLQNKNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIVEPSCKATITK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDS FCTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSAFCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQIE+WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNS GS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSTGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGA GANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGACGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. NCBI nr
Match: KAG6582497.1 (5-oxoprolinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2382 bits (6174), Expect = 0.0
Identity = 1183/1266 (93.44%), Postives = 1223/1266 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS NEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 406  MGSSNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 465

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 466  GGKKIPRTSKIPTHNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 525

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKGVSG LI +VKTLNEEALK
Sbjct: 526  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 585

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 586  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 645

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 646  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 705

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 706  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 765

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 766  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 825

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 826  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 885

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 886  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 945

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGTDTAIMV+ 
Sbjct: 946  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 1005

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK D+GVGFDFAA F KLFQQEYGF+LQNRNILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 1006 QKADNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 1065

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIVEPSCKATITK+GNI
Sbjct: 1066 KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 1125

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS  CTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 1126 KVEIDSAVCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 1185

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 1186 FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 1245

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 1246 TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 1305

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 1306 IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 1365

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNSAGS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 1366 QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1425

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1426 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1485

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1486 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1545

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1546 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1605

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1606 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1665

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1666 GGWGCP 1671

BLAST of MC10g1145 vs. NCBI nr
Match: KAG7018881.1 (5-oxoprolinase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2382 bits (6174), Expect = 0.0
Identity = 1183/1266 (93.44%), Postives = 1223/1266 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS NEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTHNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKGVSG LI +VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGTDTAIMV+ 
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK D+GVGFDFAA F KLFQQEYGF+LQNRNILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 601  QKADNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIVEPSCKATITK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS  CTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KVEIDSAVCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNSAGS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. ExPASy TrEMBL
Match: A0A6J1CQC7 (5-oxoprolinase OS=Momordica charantia OX=3673 GN=LOC111013351 PE=3 SV=1)

HSP 1 Score: 2540 bits (6583), Expect = 0.0
Identity = 1266/1266 (100.00%), Postives = 1266/1266 (100.00%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
            SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE
Sbjct: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG
Sbjct: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP
Sbjct: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI
Sbjct: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV
Sbjct: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN
Sbjct: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. ExPASy TrEMBL
Match: A0A6J1ISY5 (5-oxoprolinase OS=Cucurbita maxima OX=3661 GN=LOC111479649 PE=3 SV=1)

HSP 1 Score: 2383 bits (6175), Expect = 0.0
Identity = 1184/1266 (93.52%), Postives = 1223/1266 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS NEDKLRFCIDRGGTFTDVYAEIPG PDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGLPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT+NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKGVSG LI +VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGTDTAIMV+ 
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK+DDGVGFDFAA F KLFQQEYGF+LQN+NILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 601  QKVDDGVGFDFAAVFEKLFQQEYGFKLQNKNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIVEPSCKATITK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDS FCTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSAFCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQIE+WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNS GS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSTGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGA GANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGACGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. ExPASy TrEMBL
Match: A0A6J1E9D6 (5-oxoprolinase OS=Cucurbita moschata OX=3662 GN=LOC111431917 PE=3 SV=1)

HSP 1 Score: 2382 bits (6173), Expect = 0.0
Identity = 1182/1266 (93.36%), Postives = 1224/1266 (96.68%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS NEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKI RTSKIPT+NIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDVIEVDERVELVHGKGD NQDS TSYVKGVSG LI +VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGISCLAVVL+HSYTYPQHE+ALEKLA S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDES+GKVNVLFMQSDGGLA ENRFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRK  DPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES+ EV+RREASLLKQVK KL GQGF EGSIKTETYLNLRYEGTDTAIMV+ 
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            QK D+GVGFDFAA F KLFQQEYGF+LQNRNILICDIRVRGIG+TNVLKPRAFEG + DP
Sbjct: 601  QKADNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPLFKLDNLG G++I GPAIIMNGNSTVIV+PSCKATITK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVDPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS  CTEK+SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KVEIDSAVCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFP+SDEGV+PGTRRL DNLSDLHAQVAANHRGISLIKELIAQYGL+TVQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAE AVREMLKSVASRVSSNSAGS EGS ITIEE+DYMDDGSVIHLKLTIDP++GEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLVKKDKRRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGCP 1266
            GGWGCP
Sbjct: 1261 GGWGCP 1266

BLAST of MC10g1145 vs. ExPASy TrEMBL
Match: A0A5D3BVW9 (5-oxoprolinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00570 PE=3 SV=1)

HSP 1 Score: 2376 bits (6158), Expect = 0.0
Identity = 1180/1265 (93.28%), Postives = 1222/1265 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS+NE+KLRFCIDRGGTFTDVYAEIPGRPDGKV+KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKI RTSKIPT+NIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVEL+HGKGDGNQDS T YVKGVSG LIR+VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSST-YVKGVSGELIRIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGI CLAVVL+HSYTYPQHE+ALEKLA+S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFD+S+GKVNVLFMQSDGGLA E+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDKSSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLFELET KPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRKN DPSSKPMTIE+IALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEQIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES  EVSRREASLLKQVKHKL  QGFREGSIKTETYLNLRY+GTDTAIMVKG
Sbjct: 541  PYSAVYCSESFLEVSRREASLLKQVKHKLQSQGFREGSIKTETYLNLRYDGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            Q+ D+GV FDFAAEF KLFQQEYGF+LQNRNILICDIRVRG+G+TNVLKPRAFEG S DP
Sbjct: 601  QRADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPL KLDNLG G+II GPAIIMNGNSTVIVEPSCKAT+TKYGNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLLKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDSTFCTE +SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSTFCTENVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFPSSDEGV+PGTRRLQDNLSDLHAQVAANHRGISLIKELI QYGL+ VQAYMTYV
Sbjct: 901  IKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAEEAVREMLKSVASRVSSNSA S EG  I IEE+DYMDDGS IHLKLTIDP +GEAN
Sbjct: 961  QLNAEEAVREMLKSVASRVSSNSARSVEGGSIVIEEEDYMDDGSAIHLKLTIDPNKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGLKGGKDGA GANFLV+KD RRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLKGGKDGAHGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1265
            GGWGC
Sbjct: 1261 GGWGC 1264

BLAST of MC10g1145 vs. ExPASy TrEMBL
Match: A0A1S3C7K4 (5-oxoprolinase OS=Cucumis melo OX=3656 GN=LOC103497767 PE=3 SV=1)

HSP 1 Score: 2376 bits (6158), Expect = 0.0
Identity = 1180/1265 (93.28%), Postives = 1222/1265 (96.60%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS+NE+KLRFCIDRGGTFTDVYAEIPGRPDGKV+KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKI RTSKIPT+NIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVEL+HGKGDGNQDS T YVKGVSG LIR+VKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSST-YVKGVSGELIRIVKTLNEEALK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+DLLQRGI CLAVVL+HSYTYPQHE+ALEKLA+S+GFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFD+S+GKVNVLFMQSDGGLA E+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDKSSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLFELET KPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLA+EINSYRKN DPSSKPMTIE+IALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEQIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIF+HRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVYCSES  EVSRREASLLKQVKHKL  QGFREGSIKTETYLNLRY+GTDTAIMVKG
Sbjct: 541  PYSAVYCSESFLEVSRREASLLKQVKHKLQSQGFREGSIKTETYLNLRYDGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            Q+ D+GV FDFAAEF KLFQQEYGF+LQNRNILICDIRVRG+G+TNVLKPRAFEG S DP
Sbjct: 601  QRADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            KIEGH +VYFG+GW++TPL KLDNLG G+II GPAIIMNGNSTVIVEPSCKAT+TKYGNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLLKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDSTFCTE +SEKV DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSTFCTENVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDN KLIFFVASRGHHAEIGGITPGSMPPFSK IWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  IKLLQFPSSDEGVVPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMTYV 960
            IKLLQFPSSDEGV+PGTRRLQDNLSDLHAQVAANHRGISLIKELI QYGL+ VQAYMTYV
Sbjct: 901  IKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGEAN 1020
            QLNAEEAVREMLKSVASRVSSNSA S EG  I IEE+DYMDDGS IHLKLTIDP +GEAN
Sbjct: 961  QLNAEEAVREMLKSVASRVSSNSARSVEGGSIVIEEEDYMDDGSAIHLKLTIDPNKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP GS LSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPCW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGP W
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGG+GVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTPGG 1260
            VVSILSERRVHAPRGLKGGKDGA GANFLV+KD RRVYLGGKN+VTV+AGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLKGGKDGAHGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1265
            GGWGC
Sbjct: 1261 GGWGC 1264

BLAST of MC10g1145 vs. TAIR 10
Match: AT5G37830.1 (oxoprolinase 1 )

HSP 1 Score: 2124.7 bits (5504), Expect = 0.0e+00
Identity = 1038/1266 (81.99%), Postives = 1145/1266 (90.44%), Query Frame = 0

Query: 1    MGSDNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60
            MG+  E KLRFCIDRGGTFTDVYAEIPG  DG V+KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGTVIEGKLRFCIDRGGTFTDVYAEIPGHSDGHVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKISRTSKIPTENIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKI RTSKIPT+ I+WIRMGTTVATNALLERKGERIALCVTKGF+DLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTDKIQWIRMGTTVATNALLERKGERIALCVTKGFKDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDGNQDSYTSYVKGVSGGLIRVVKTLNEEALK 180
            FDLTV+KPSNLYE+VIEVDERV L     + + D   S +KGVSG  +RVVK  + E LK
Sbjct: 121  FDLTVAKPSNLYEEVIEVDERVVLAL---EDDDDDEGSLIKGVSGEFLRVVKPFDGEGLK 180

Query: 181  SSLEDLLQRGISCLAVVLLHSYTYPQHEIALEKLAMSLGFKHVSLSSALTPMVRAVPRGL 240
              L+ LL +GISCLAVVL+HSYTYP+HE+ +EKLA+ +GF+HVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKGLLDKGISCLAVVLMHSYTYPKHEMDVEKLALEMGFRHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESNGKVNVLFMQSDGGLALENRFSGHKAVLSGPAGG 300
            TA+VDAYLTPVIKEYLSGF+SKFD+  GKVNVLFMQSDGGLA E+RFSGHKAVLSGPAGG
Sbjct: 241  TATVDAYLTPVIKEYLSGFISKFDDDLGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF LET KPLIGFDMGGTSTDVSRY GSYEQV+ETQIAG IIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFGLETEKPLIGFDMGGTSTDVSRYDGSYEQVIETQIAGTIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFWVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPE 420
            AGGGSKLKFQFGAF VGP+SVGAHPGPVCYRKGGELAVTDANLVLGFVIPD+FPSIFGP 
Sbjct: 361  AGGGSKLKFQFGAFRVGPDSVGAHPGPVCYRKGGELAVTDANLVLGFVIPDYFPSIFGPN 420

Query: 421  EDQPLDIEATRGQFEKLASEINSYRKNHDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLD+ ATR  FEKLA +IN YRK+ DPS+K M++EEIA+GFV+VANETMCRPIRQLT
Sbjct: 421  EDQPLDVAATREAFEKLAGQINIYRKSQDPSAKDMSVEEIAMGFVSVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFVHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIAR LGMKE+ VHR+CGILSAYGMGLADV+E+ QE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARSLGMKEVLVHRYCGILSAYGMGLADVIEDAQE 540

Query: 541  PYSAVYCSESVHEVSRREASLLKQVKHKLLGQGFREGSIKTETYLNLRYEGTDTAIMVKG 600
            PYSAVY  ES+ EV RRE  LL++V+ KL  QGF +G+I TETYLNLRY+GTDTAIMVKG
Sbjct: 541  PYSAVYGPESLSEVFRRETVLLREVREKLQEQGFGDGNISTETYLNLRYDGTDTAIMVKG 600

Query: 601  QKIDDGVGFDFAAEFVKLFQQEYGFELQNRNILICDIRVRGIGITNVLKPRAFEGRSSDP 660
            +K  DG  FD+AAEF+KLF+QEYGF+LQNRN+LICD+RVRGIG+T++LKPRA E     P
Sbjct: 601  KKTGDGSAFDYAAEFLKLFEQEYGFKLQNRNLLICDVRVRGIGVTSILKPRAVEAAPVTP 660

Query: 661  KIEGHCKVYFGDGWKNTPLFKLDNLGCGNIILGPAIIMNGNSTVIVEPSCKATITKYGNI 720
            K+E H KVYF  GW +TPLFKL+NLG G+ ILGPAIIMNGNSTVIVEP CKA ITKYGNI
Sbjct: 661  KVERHYKVYFEGGWHDTPLFKLENLGFGHEILGPAIIMNGNSTVIVEPQCKAIITKYGNI 720

Query: 721  KIEIDSTFCTEKISEKVVDVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIE++    + K++E V DVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEVEPATSSVKLAENVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            F PDGGLVANAPHVPVHLGAMSSTVRWQ++ WG+NLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FSPDGGLVANAPHVPVHLGAMSSTVRWQLKHWGENLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNEKLIFFVASRGHHAEIGGITPGSMPPFSKFIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFD  KL+FFVASRGHHAE+GGITPGSMPPFSK IWEEGAAIKAFK+VEKG+FQEEGI
Sbjct: 841  TPVFDKGKLVFFVASRGHHAEVGGITPGSMPPFSKAIWEEGAAIKAFKVVEKGVFQEEGI 900

Query: 901  IKLLQFPSSDEGV--VPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLSTVQAYMT 960
            +KLLQFPSSDE    +PGTRR+QDNLSDL AQ+AAN RGISLIKELI QYGL TVQAYM 
Sbjct: 901  VKLLQFPSSDETTTKIPGTRRIQDNLSDLQAQIAANQRGISLIKELIEQYGLGTVQAYMK 960

Query: 961  YVQLNAEEAVREMLKSVASRVSSNSAGSEEGSPITIEEDDYMDDGSVIHLKLTIDPQRGE 1020
            YVQLNAEEAVREMLKSVA+RVSS +  S  G+ +TIEE+DYMDDGS+IHLKLTID  +GE
Sbjct: 961  YVQLNAEEAVREMLKSVANRVSSETPNSRVGNSVTIEEEDYMDDGSIIHLKLTIDADKGE 1020

Query: 1021 ANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPSGSLLS 1080
            A+FDF+GTSPEVYGNWNAPEAVT+AAVIYCLRC+V+VDIPLNQGCLAPV+I IP+GS LS
Sbjct: 1021 ASFDFTGTSPEVYGNWNAPEAVTSAAVIYCLRCLVNVDIPLNQGCLAPVEIRIPAGSFLS 1080

Query: 1081 PSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGP 1140
            PSEKAA+VGGNVLTSQR+TDV+LTAFQACACSQGCMNNLTFGDDTFGYYETIGGG GAGP
Sbjct: 1081 PSEKAAVVGGNVLTSQRVTDVVLTAFQACACSQGCMNNLTFGDDTFGYYETIGGGCGAGP 1140

Query: 1141 CWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGNGVYKGGDGLVREIEFKQ 1200
             W+GTSGVQCHMTNTRMTDPEIFEQRYPVLLH F LRENSGGNG++KGGDGLVREIEF++
Sbjct: 1141 TWNGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFGLRENSGGNGLHKGGDGLVREIEFRK 1200

Query: 1201 PVVVSILSERRVHAPRGLKGGKDGARGANFLVKKDKRRVYLGGKNSVTVRAGEILQILTP 1260
            PVVVSILSERRVH+PRGL GG++G RGAN+L+ KDKRR+YLGGKN+V V AGEILQILTP
Sbjct: 1201 PVVVSILSERRVHSPRGLNGGQNGLRGANYLITKDKRRIYLGGKNTVHVEAGEILQILTP 1260

Query: 1261 GGGGWG 1265
            GGGG+G
Sbjct: 1261 GGGGFG 1263