MC08g2527 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2527 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.6e-182
Identity = 320/456 (70.18%), Postives = 382/456 (83.77%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E ++EAKSLF+LAFPIA+TAL++Y RS +SM FLG+LGD+ELAAGSLAIAFANITGYSVL
Sbjct: 52  EAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVL 111

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           SGLALGMEPLCSQAFGAHR KLLSLTLHR V+FLLV  +PIS+LW  + KI ++L QDP 
Sbjct: 112 SGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPD 171

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I ++AQTYLIFSLP+L+TN+ ++PIRIYLRAQG+  P+TLASL+GAVFH+P NL LVS+ 
Sbjct: 172 IAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYL 231

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
             G+ GVA A++ TN  V+ FLV YV  SG+HAPTWT P+R+C  GW PLL LA PSCVS
Sbjct: 232 RLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVS 291

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYEIMIVLCGLLV P++TVA+MGVLIQTTS +Y+FPSSL FAVSTRVGNELGA 
Sbjct: 292 VCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGAN 351

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP  AKL+A VA+  AA+ G+ A  FA  +RN W R+FT D EIL+LT+ ALPILGLCE+
Sbjct: 352 RPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEI 411

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQTVGCGV+RG+ARPSTAAN+NLGAFY VGMPVAVGL F  G+GF GLW+GLL+AQ+
Sbjct: 412 GNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQI 471

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTCTAGEELPLI 505
            CAGLM+YV+GTTDW+ +A ++  LTC    E  +I
Sbjct: 472 SCAGLMMYVVGTTDWESEAKKAQTLTCAETVENDII 507

BLAST of MC08g2527 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.4e-175
Identity = 304/461 (65.94%), Postives = 379/461 (82.21%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E+ SEA+SLFSLAFP  L ALI+Y+RS ISMLFLG +G++ELA GSLAIAFANITGYSVL
Sbjct: 32  ELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVL 91

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           +GLALGM+PLCSQAFGA RPKLLSLTL R V+FLL SS+ I  LW+ + KI+++L QDP 
Sbjct: 92  AGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPS 151

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I+ +AQTY++ S+P+L+TNSF++P+RIYLRAQG+T PLTLA+LAG +FH+P+N  LVS+ 
Sbjct: 152 ISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYL 211

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
            +G  GV+ AAAA+N LV++FLVA+V ++G+H PTWT PS EC   W P++ LA PSC+ 
Sbjct: 212 GWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIG 271

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYEIM VLCGLL+ P   VASMG+LIQTTSL+YIFPSSLG AVSTRVGNELG+ 
Sbjct: 272 VCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSN 331

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP+KA+LSA+VAV  A ++GL A+ FA G+ + W  +FT+D  I++LT+ ALPILGLCEL
Sbjct: 332 RPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCEL 391

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQTVGCGV+RG+ARPS AANINLGAFY VG PVAVGL F    GFCGLW+GLL+AQ+
Sbjct: 392 GNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQI 451

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTCTAGEELPLISPPHN 510
           CCA +MLYV+ TTDW+ +A R+  LTCT G ++ + +   N
Sbjct: 452 CCAAMMLYVVATTDWEKEAIRARKLTCTEGVDVVITTTQTN 492

BLAST of MC08g2527 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.3e-141
Identity = 259/457 (56.67%), Postives = 337/457 (73.74%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E + E K++  ++ P A+T L++YSR++ISMLFLG LG++ELA GSL+I FANITGYSV+
Sbjct: 54  EGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVI 113

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           SGL++GMEP+C QA+GA + KLL LTL R V+ LL  S+PIS  W+ M +ILL+  QD  
Sbjct: 114 SGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEE 173

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I+ +AQ +L+F++P+L   S ++P+RIYLR Q +T+P+T ++    + HVP+N LLV   
Sbjct: 174 ISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKL 233

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
           E GVAGVA A   TN  ++V L ++V  + VH+ TW   + + L GW  LL LA P+CVS
Sbjct: 234 EMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVS 293

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYE MI+LCGLL  P+ATVASMG+LIQTT+L+Y+FPSSL   VSTR+ NELGA 
Sbjct: 294 VCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAK 353

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP KA++S ++++F A  LGL A +FA  +R+ W R+FT DAEIL+LTS+ALPI+GLCEL
Sbjct: 354 RPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCEL 413

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQT GCGVLRG ARP+  ANINLG+FY VGMPVA+   F    GF GLW GLL+AQ 
Sbjct: 414 GNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQA 473

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTC-TAGEELPLI 505
            CA LML  +  TDW  QA R+  LT  T G+  PL+
Sbjct: 474 TCASLMLCALLRTDWKVQAERAEELTSQTPGKSPPLL 510

BLAST of MC08g2527 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.2e-141
Identity = 264/454 (58.15%), Postives = 337/454 (74.23%), Query Frame = 0

Query: 51  ISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIE-LAAGSLAIAFANITGYSVLS 110
           I EAKS+  ++ P+ LT L++YSRS+ISMLFLGRL D+  L+ GSLA+ FANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 111 GLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGI 170
           GL++GMEP+C QAFGA R KLL L L R  + LL+ S+PIS+LW+ + KILLF  QD  I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 171 TEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFE 230
           +  A+ +++FSLP+L+  SF++PIRIYLR+Q +T+PLT ++    + H+PIN LLVS   
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 231 FGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSV 290
            G+ GVA  A  TN  +L FL+ Y+V SGV+  TW   S +C  GW+ L++LA PSCVSV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 291 CLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGR 350
           CLEWWWYEIMI+LCGLL+ P+ATVASMG+LIQTT+LIYIFPSSL  +VSTRVGNELGA +
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 351 PHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELG 410
           P KA+++A   + ++  LGL A  FA  +RN WAR+FTD+ EI++LTS+ LPI+GLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 411 NCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVC 470
           NCPQT  CGVLRGSARP   ANINL  FY VGMPVAV L+F  G  F GLWLGL +AQ  
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 471 CAGLMLYVIGTTDWDFQACRSHML---TCTAGEE 501
           C   ML V+  TDW+ +  R+  L   +C   E+
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKELMTRSCDGDED 483

BLAST of MC08g2527 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 3.6e-123
Identity = 229/450 (50.89%), Postives = 311/450 (69.11%), Query Frame = 0

Query: 51  ISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSG 110
           ++EA S+  +++P+ LT L +Y RS +S+ FLG LGD  LA GSLA AFANITGYS+ SG
Sbjct: 33  LNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYSLFSG 92

Query: 111 LALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGIT 170
           L +G+E +CSQAFGA R   +  ++ R +I LLV+S+P++LLW+ M KILL L+QD  + 
Sbjct: 93  LTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQDKKLA 152

Query: 171 EMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEF 230
             A  +L++S+P+LV  SF++P+R+YLR Q  T+PL++ ++  +  H+PI   LVS+   
Sbjct: 153 SEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVSYLGL 212

Query: 231 GVAGVAAAAAATNFLVLVFLVAYV------VVSGVHAPTWTAPSRECLSGWKPLLELAAP 290
           G+ G+A +   +NF ++ FL  Y+      +              + +  WK LL LA P
Sbjct: 213 GIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLCLAIP 272

Query: 291 SCVSVCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNE 350
           SC+SVCLEWW YEIMI+LCG L+ PKA+VASMG+LIQ TSL+YIFP SL   VSTRVGNE
Sbjct: 273 SCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTRVGNE 332

Query: 351 LGAGRPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILG 410
           LG+ +P +A+ +A+V + ++  LG  A  F   +RN WA  FTDD EI++LT++ALPI+G
Sbjct: 333 LGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMALPIVG 392

Query: 411 LCELGNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLL 470
           LCELGNCPQT GCGVLRGSARP   ANIN  AFY VG+PV   LAF  G GF GLWLG+L
Sbjct: 393 LCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLWLGML 452

Query: 471 SAQVCCAGLMLYVIGTTDWDFQACRSHMLT 495
           +AQ+ C   M+     TDW+ +A R+ +LT
Sbjct: 453 AAQITCVIGMMAATCRTDWELEAERAKVLT 482

BLAST of MC08g2527 vs. NCBI nr
Match: XP_022154665.1 (protein DETOXIFICATION 51 [Momordica charantia])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL 60
           MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL
Sbjct: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL 60

Query: 61  AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS 120
           AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS
Sbjct: 61  AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS 120

Query: 121 QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS 180
           QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS
Sbjct: 121 QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS 180

Query: 181 LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA 240
           LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA
Sbjct: 181 LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA 240

Query: 241 ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI 300
           ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI
Sbjct: 241 ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI 300

Query: 301 VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA 360
           VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA
Sbjct: 301 VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA 360

Query: 361 VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL 420
           VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL
Sbjct: 361 VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL 420

Query: 421 RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT 480
           RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT
Sbjct: 421 RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT 480

Query: 481 TDWDFQACRSHMLTCTAGEELPLISPPHN 509
           TDWDFQACRSHMLTCTAGEELPLISPPHN
Sbjct: 481 TDWDFQACRSHMLTCTAGEELPLISPPHN 509

BLAST of MC08g2527 vs. NCBI nr
Match: KAG7021311.1 (Protein DETOXIFICATION 51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 858 bits (2217), Expect = 2.32e-311
Identity = 453/514 (88.13%), Postives = 474/514 (92.22%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSW-------EIISE 60
           MCVSIS+ APA    P P  S     PP++ Q FDVFPKK+++Q FS        EII+E
Sbjct: 1   MCVSISNPAPATNCPPPPPPS-----PPELLQFFDVFPKKKSQQSFSCPTPTGLSEIITE 60

Query: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120
           AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL
Sbjct: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120

Query: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMA 180
           GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSS+PISLLWVKM KILLFLRQDP IT+MA
Sbjct: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSLPISLLWVKMGKILLFLRQDPSITDMA 180

Query: 181 QTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVA 240
           QTYLIFSLP+LVTNSFINPIRIYLRAQ +TVPLTLASL GA+ HVPIN LLVS+FEFGVA
Sbjct: 181 QTYLIFSLPDLVTNSFINPIRIYLRAQSITVPLTLASLGGALCHVPINFLLVSYFEFGVA 240

Query: 241 GVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300
           GVAA+AAATNFL+LVFLV YVVVSGVH PTWTAPSRECLSGWKPLLELAAPSCVSVCLEW
Sbjct: 241 GVAASAAATNFLMLVFLVVYVVVSGVHVPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300

Query: 301 WWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKA 360
           WWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KA
Sbjct: 301 WWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPEKA 360

Query: 361 KLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQ 420
           KLSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDDAEILRLT+VALPILGLCELGNCPQ
Sbjct: 361 KLSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDAEILRLTAVALPILGLCELGNCPQ 420

Query: 421 TVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGL 480
           TVGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAF LGVGFCGLWLGLLSAQVCCAGL
Sbjct: 421 TVGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFSLGVGFCGLWLGLLSAQVCCAGL 480

Query: 481 MLYVIGTTDWDFQACRSHMLTCTAGEELPLISPP 507
           MLYV+GTTDW+FQACRSH+LTCT G+ELPL SPP
Sbjct: 481 MLYVVGTTDWEFQACRSHVLTCTVGDELPLFSPP 509

BLAST of MC08g2527 vs. NCBI nr
Match: XP_038890722.1 (protein DETOXIFICATION 51-like [Benincasa hispida])

HSP 1 Score: 857 bits (2215), Expect = 4.86e-311
Identity = 461/522 (88.31%), Postives = 479/522 (91.76%), Query Frame = 0

Query: 1   MCVSIS--SSAPA---PAPAPAPTLSSSKFPPPQIFQLFDVFPKKQT------EQFFSW- 60
           MCVSIS  SSAPA   P P P P       PP Q+   FDVFPKKQ       +Q FS+ 
Sbjct: 1   MCVSISNPSSAPATNFPPPPPPP-------PPSQLLHFFDVFPKKQPPPPPPPQQLFSFS 60

Query: 61  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 120
           EII+EAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGD+ELAAGSLAIAFANITGYSVL
Sbjct: 61  EIITEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDMELAAGSLAIAFANITGYSVL 120

Query: 121 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 180
           SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSS+PISLLWVKM+KILL LRQDP 
Sbjct: 121 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSLPISLLWVKMSKILLLLRQDPS 180

Query: 181 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 240
           IT MAQTYL FSLP+LVTNSFINPIRIYLRAQG+TVPLTLASL GA+ HVPIN+LLVS+F
Sbjct: 181 ITAMAQTYLFFSLPDLVTNSFINPIRIYLRAQGITVPLTLASLGGALCHVPINILLVSYF 240

Query: 241 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 300
           EFGVAGVAA+AAATNFLVLVFLV YVVVS VHAPTWTAPSRECLSGWKPLLELAAPSC+S
Sbjct: 241 EFGVAGVAASAAATNFLVLVFLVVYVVVSDVHAPTWTAPSRECLSGWKPLLELAAPSCIS 300

Query: 301 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 360
           VCLEWWWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG G
Sbjct: 301 VCLEWWWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGG 360

Query: 361 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 420
           RP KAKLSAVVAVFVAA++GLGAT FATGMRNKWARMFTDD EILRLTSVALPILGLCEL
Sbjct: 361 RPQKAKLSAVVAVFVAAIMGLGATSFATGMRNKWARMFTDDGEILRLTSVALPILGLCEL 420

Query: 421 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 480
           GNCPQTVGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV
Sbjct: 421 GNCPQTVGCGVLRGSARPSVAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 480

Query: 481 CCAGLMLYVIGTTDWDFQACRSHMLTCTAGEELPLISPP-HN 509
           CCAGLMLYVIGTTDWDFQACRSH+LTCTAG+ELPLISPP HN
Sbjct: 481 CCAGLMLYVIGTTDWDFQACRSHVLTCTAGDELPLISPPTHN 515

BLAST of MC08g2527 vs. NCBI nr
Match: XP_023537997.1 (protein DETOXIFICATION 51-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857 bits (2213), Expect = 9.44e-311
Identity = 452/514 (87.94%), Postives = 474/514 (92.22%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSW-------EIISE 60
           MCVSIS+ APA    P P  S     PP++ Q FDVFPKK+++Q FS        EII+E
Sbjct: 1   MCVSISNPAPATNCPPPPPPS-----PPELLQFFDVFPKKKSQQSFSCPTPTGLSEIITE 60

Query: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120
           AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL
Sbjct: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120

Query: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMA 180
           GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSS+PISLLWVKM KILLFLRQDP IT+MA
Sbjct: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSLPISLLWVKMGKILLFLRQDPSITDMA 180

Query: 181 QTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVA 240
           QTYLIFSLP+LVTNSFINPIRIYLRAQ +TVPLTLASL GA+ HVPIN LLVS+FEFGVA
Sbjct: 181 QTYLIFSLPDLVTNSFINPIRIYLRAQSITVPLTLASLGGALCHVPINFLLVSYFEFGVA 240

Query: 241 GVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300
           GVAA+AAATNFL+LVFLV YVVVSGVH PTWTAPSRECLSGWKPLLELAAPSCVSVCLEW
Sbjct: 241 GVAASAAATNFLMLVFLVVYVVVSGVHVPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300

Query: 301 WWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKA 360
           WWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KA
Sbjct: 301 WWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPEKA 360

Query: 361 KLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQ 420
           KLSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDDAEILRLT+VALPILGLCELGNCPQ
Sbjct: 361 KLSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDAEILRLTAVALPILGLCELGNCPQ 420

Query: 421 TVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGL 480
           TVGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAF LGVGFCGLWLGLLSAQVCCAGL
Sbjct: 421 TVGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFSLGVGFCGLWLGLLSAQVCCAGL 480

Query: 481 MLYVIGTTDWDFQACRSHMLTCTAGEELPLISPP 507
           MLYV+GTTDW+FQACRSH+LTC+ G+ELPL SPP
Sbjct: 481 MLYVVGTTDWEFQACRSHVLTCSVGDELPLFSPP 509

BLAST of MC08g2527 vs. NCBI nr
Match: XP_022938295.1 (protein DETOXIFICATION 51-like [Cucurbita moschata])

HSP 1 Score: 854 bits (2206), Expect = 1.10e-309
Identity = 450/514 (87.55%), Postives = 473/514 (92.02%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSW-------EIISE 60
           MCVSIS+ APA    P P  S     PP++ Q FDVFPKK+++Q FS        EII+E
Sbjct: 1   MCVSISNPAPATNCPPPPPPS-----PPELLQFFDVFPKKKSQQSFSCPTPTRLSEIITE 60

Query: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120
           AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITG+SVLSGLAL
Sbjct: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGHSVLSGLAL 120

Query: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMA 180
           GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSS+PISLLWVKM KILLFLRQDP IT+MA
Sbjct: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSLPISLLWVKMGKILLFLRQDPSITDMA 180

Query: 181 QTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVA 240
           QTYLIFSLP+LVTNSFINPIRIYLRAQ +T PLTLASL GA+ HVPIN LLVS+FEFGVA
Sbjct: 181 QTYLIFSLPDLVTNSFINPIRIYLRAQSITFPLTLASLGGALCHVPINFLLVSYFEFGVA 240

Query: 241 GVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300
           GVAA+AAATNFL+L+FLV YVVVSGVH PTWTAPSRECLSGWKPLLELAAPSCVSVCLEW
Sbjct: 241 GVAASAAATNFLMLIFLVVYVVVSGVHVPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300

Query: 301 WWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKA 360
           WWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KA
Sbjct: 301 WWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPEKA 360

Query: 361 KLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQ 420
           KLSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDDAEILRLT+VALPILGLCELGNCPQ
Sbjct: 361 KLSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDAEILRLTAVALPILGLCELGNCPQ 420

Query: 421 TVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGL 480
           TVGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAF LGVGFCGLWLGLLSAQVCCAGL
Sbjct: 421 TVGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFSLGVGFCGLWLGLLSAQVCCAGL 480

Query: 481 MLYVIGTTDWDFQACRSHMLTCTAGEELPLISPP 507
           MLYV+GTTDW+FQACRSH+LTCT G+ELPL SPP
Sbjct: 481 MLYVVGTTDWEFQACRSHVLTCTVGDELPLFSPP 509

BLAST of MC08g2527 vs. ExPASy TrEMBL
Match: A0A6J1DK95 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111021868 PE=3 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL 60
           MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL
Sbjct: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSWEIISEAKSLFSL 60

Query: 61  AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS 120
           AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS
Sbjct: 61  AFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALGMEPLCS 120

Query: 121 QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS 180
           QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS
Sbjct: 121 QAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQTYLIFS 180

Query: 181 LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA 240
           LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA
Sbjct: 181 LPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAGVAAAAA 240

Query: 241 ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI 300
           ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI
Sbjct: 241 ATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWWWYEIMI 300

Query: 301 VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA 360
           VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA
Sbjct: 301 VLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAKLSAVVA 360

Query: 361 VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL 420
           VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL
Sbjct: 361 VFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQTVGCGVL 420

Query: 421 RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT 480
           RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT
Sbjct: 421 RGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLMLYVIGT 480

Query: 481 TDWDFQACRSHMLTCTAGEELPLISPPHN 509
           TDWDFQACRSHMLTCTAGEELPLISPPHN
Sbjct: 481 TDWDFQACRSHMLTCTAGEELPLISPPHN 509

BLAST of MC08g2527 vs. ExPASy TrEMBL
Match: A0A6J1FJD0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111444429 PE=3 SV=1)

HSP 1 Score: 854 bits (2206), Expect = 5.32e-310
Identity = 450/514 (87.55%), Postives = 473/514 (92.02%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQTEQFFSW-------EIISE 60
           MCVSIS+ APA    P P  S     PP++ Q FDVFPKK+++Q FS        EII+E
Sbjct: 1   MCVSISNPAPATNCPPPPPPS-----PPELLQFFDVFPKKKSQQSFSCPTPTRLSEIITE 60

Query: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLAL 120
           AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITG+SVLSGLAL
Sbjct: 61  AKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGHSVLSGLAL 120

Query: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMA 180
           GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSS+PISLLWVKM KILLFLRQDP IT+MA
Sbjct: 121 GMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSLPISLLWVKMGKILLFLRQDPSITDMA 180

Query: 181 QTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVA 240
           QTYLIFSLP+LVTNSFINPIRIYLRAQ +T PLTLASL GA+ HVPIN LLVS+FEFGVA
Sbjct: 181 QTYLIFSLPDLVTNSFINPIRIYLRAQSITFPLTLASLGGALCHVPINFLLVSYFEFGVA 240

Query: 241 GVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300
           GVAA+AAATNFL+L+FLV YVVVSGVH PTWTAPSRECLSGWKPLLELAAPSCVSVCLEW
Sbjct: 241 GVAASAAATNFLMLIFLVVYVVVSGVHVPTWTAPSRECLSGWKPLLELAAPSCVSVCLEW 300

Query: 301 WWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKA 360
           WWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KA
Sbjct: 301 WWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPEKA 360

Query: 361 KLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQ 420
           KLSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDDAEILRLT+VALPILGLCELGNCPQ
Sbjct: 361 KLSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDAEILRLTAVALPILGLCELGNCPQ 420

Query: 421 TVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGL 480
           TVGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAF LGVGFCGLWLGLLSAQVCCAGL
Sbjct: 421 TVGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFSLGVGFCGLWLGLLSAQVCCAGL 480

Query: 481 MLYVIGTTDWDFQACRSHMLTCTAGEELPLISPP 507
           MLYV+GTTDW+FQACRSH+LTCT G+ELPL SPP
Sbjct: 481 MLYVVGTTDWEFQACRSHVLTCTVGDELPLFSPP 509

BLAST of MC08g2527 vs. ExPASy TrEMBL
Match: A0A5A7V059 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00620 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 1.79e-309
Identity = 451/516 (87.40%), Postives = 473/516 (91.67%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQT-----EQFFSW-EIISEA 60
           MC+SIS+ +  P P P P       PP Q+   FD+FPKKQ      +  FS  EI+SEA
Sbjct: 1   MCLSISNPSSPPPPPPPP-------PPSQLLHFFDLFPKKQLPPPPPQHLFSCSEIVSEA 60

Query: 61  KSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALG 120
           KSLFSLAFPIALTALIIYSRSIISM+FLGRLGD+ELAAGSLAIAFANITGYSVLSGLALG
Sbjct: 61  KSLFSLAFPIALTALIIYSRSIISMIFLGRLGDMELAAGSLAIAFANITGYSVLSGLALG 120

Query: 121 MEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQ 180
           MEPLCSQAFGA RPKLLSLTLHRAVIFLLVSSIPI +LWV M KILLFLRQDP ITEMAQ
Sbjct: 121 MEPLCSQAFGAQRPKLLSLTLHRAVIFLLVSSIPIFILWVNMGKILLFLRQDPSITEMAQ 180

Query: 181 TYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAG 240
           TYLIFSLP+LV NSFINPIRIYLRAQG+TVPLTLASL GA+ H+PIN LLVS+F+FGVAG
Sbjct: 181 TYLIFSLPDLVANSFINPIRIYLRAQGITVPLTLASLGGALCHMPINFLLVSYFDFGVAG 240

Query: 241 VAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWW 300
           VAA+AAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSC+SVCLEWW
Sbjct: 241 VAASAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCISVCLEWW 300

Query: 301 WYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAK 360
           WYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KAK
Sbjct: 301 WYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPQKAK 360

Query: 361 LSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQT 420
           LSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDD EILRLTSVALPILGLCELGNCPQT
Sbjct: 361 LSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDGEILRLTSVALPILGLCELGNCPQT 420

Query: 421 VGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM 480
           VGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM
Sbjct: 421 VGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM 480

Query: 481 LYVIGTTDWDFQACRSHMLTCTAGEELPLISPP-HN 509
           LYVIGTTDWDFQACRSH+LTCTAG+ELPL+SPP HN
Sbjct: 481 LYVIGTTDWDFQACRSHLLTCTAGDELPLLSPPSHN 509

BLAST of MC08g2527 vs. ExPASy TrEMBL
Match: A0A1S3C919 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103498204 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 1.79e-309
Identity = 452/516 (87.60%), Postives = 473/516 (91.67%), Query Frame = 0

Query: 1   MCVSISSSAPAPAPAPAPTLSSSKFPPPQIFQLFDVFPKKQT-----EQFFSW-EIISEA 60
           MC+SIS+ +  P P P P       PP Q+   FD+FPKKQ      +  FS  EI+SEA
Sbjct: 1   MCLSISNPSSPPPPPPPP-------PPSQLLHFFDLFPKKQLPPPPPQHLFSCSEIVSEA 60

Query: 61  KSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLALG 120
           KSLFSLAFPIALTALIIYSRSIISM+FLGRLGD+ELAAGSLAIAFANITGYSVLSGLALG
Sbjct: 61  KSLFSLAFPIALTALIIYSRSIISMIFLGRLGDMELAAGSLAIAFANITGYSVLSGLALG 120

Query: 121 MEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEMAQ 180
           MEPLCSQAFGA RPKLLSLTLHRAVIFLLVSSIPI +LWV M KILLFLRQDP ITEMAQ
Sbjct: 121 MEPLCSQAFGAQRPKLLSLTLHRAVIFLLVSSIPIFILWVNMGKILLFLRQDPSITEMAQ 180

Query: 181 TYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGVAG 240
           TYLIFSLP+LVTNSFINPIRIYLRAQG+TVPLTLASL GA+ H+PIN LLVS F+FGVAG
Sbjct: 181 TYLIFSLPDLVTNSFINPIRIYLRAQGITVPLTLASLGGALCHMPINFLLVSCFDFGVAG 240

Query: 241 VAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLEWW 300
           VAA+AAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSC+SVCLEWW
Sbjct: 241 VAASAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCISVCLEWW 300

Query: 301 WYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHKAK 360
           WYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG GRP KAK
Sbjct: 301 WYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGGRPQKAK 360

Query: 361 LSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCPQT 420
           LSAVVAVFVAAM+GLGAT FATGMRNKWARMFTDD EILRLTSVALPILGLCELGNCPQT
Sbjct: 361 LSAVVAVFVAAMMGLGATSFATGMRNKWARMFTDDGEILRLTSVALPILGLCELGNCPQT 420

Query: 421 VGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM 480
           VGCGVLRGSARPS AANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM
Sbjct: 421 VGCGVLRGSARPSAAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAGLM 480

Query: 481 LYVIGTTDWDFQACRSHMLTCTAGEELPLISPP-HN 509
           LYVIGTTDWDFQACRSH+LTCTAG+ELPL+SPP HN
Sbjct: 481 LYVIGTTDWDFQACRSHLLTCTAGDELPLLSPPSHN 509

BLAST of MC08g2527 vs. ExPASy TrEMBL
Match: A0A6J1G2J3 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111450242 PE=3 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 2.85e-309
Identity = 451/516 (87.40%), Postives = 476/516 (92.25%), Query Frame = 0

Query: 1   MCVSISSSAPAPA---PAPAPTLSSSKFPPPQIFQLFDVFPKKQT----EQFFSW-EIIS 60
           MCVSIS+ + APA   P P P  S     P ++ Q FDVFPKKQ     +Q FS  EI++
Sbjct: 1   MCVSISNPSSAPATSCPPPPPPTS-----PSELLQFFDVFPKKQPPQPKQQLFSCSEIVT 60

Query: 61  EAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSGLA 120
           EAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGD+ELAAGSLAIAFANITGYSVLSGLA
Sbjct: 61  EAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDVELAAGSLAIAFANITGYSVLSGLA 120

Query: 121 LGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGITEM 180
           LGMEPLCSQAFGAHRPKLLSLTLHR+VIFLLV SIPISLLWVKMAKILLFLRQDP ITEM
Sbjct: 121 LGMEPLCSQAFGAHRPKLLSLTLHRSVIFLLVCSIPISLLWVKMAKILLFLRQDPTITEM 180

Query: 181 AQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEFGV 240
           AQTYL+FSLP+L+TNSFINPIRIYLRAQG+TVPLTLASL GA+ HVPIN LLVS+++FGV
Sbjct: 181 AQTYLVFSLPDLITNSFINPIRIYLRAQGITVPLTLASLGGALCHVPINFLLVSYYKFGV 240

Query: 241 AGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSVCLE 300
           AGVAA+AAATNFLVLVFLV YVVVSGVHAPTWT PSRECL+GWKPLLELAAPSC+SVCLE
Sbjct: 241 AGVAASAAATNFLVLVFLVLYVVVSGVHAPTWTTPSRECLTGWKPLLELAAPSCISVCLE 300

Query: 301 WWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGRPHK 360
           WWWYEIMIVLCGLLV PKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELG  RP K
Sbjct: 301 WWWYEIMIVLCGLLVDPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGGSRPQK 360

Query: 361 AKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELGNCP 420
           AKLSAVVAVF+AAM+GLGAT FATGMRNKWARMFTDD EILRLTSVALPILGLCELGNCP
Sbjct: 361 AKLSAVVAVFMAAMMGLGATSFATGMRNKWARMFTDDREILRLTSVALPILGLCELGNCP 420

Query: 421 QTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAG 480
           QTVGCGVLRG ARPS AANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAG
Sbjct: 421 QTVGCGVLRGCARPSAAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVCCAG 480

Query: 481 LMLYVIGTTDWDFQACRSHMLTCTAGEELPLISPPH 508
           LMLYVIGTTDWDFQACRSH+LTCTAG+ELPLISPP+
Sbjct: 481 LMLYVIGTTDWDFQACRSHVLTCTAGDELPLISPPN 511

BLAST of MC08g2527 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.9e-183
Identity = 320/456 (70.18%), Postives = 382/456 (83.77%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E ++EAKSLF+LAFPIA+TAL++Y RS +SM FLG+LGD+ELAAGSLAIAFANITGYSVL
Sbjct: 52  EAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVL 111

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           SGLALGMEPLCSQAFGAHR KLLSLTLHR V+FLLV  +PIS+LW  + KI ++L QDP 
Sbjct: 112 SGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPD 171

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I ++AQTYLIFSLP+L+TN+ ++PIRIYLRAQG+  P+TLASL+GAVFH+P NL LVS+ 
Sbjct: 172 IAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYL 231

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
             G+ GVA A++ TN  V+ FLV YV  SG+HAPTWT P+R+C  GW PLL LA PSCVS
Sbjct: 232 RLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVS 291

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYEIMIVLCGLLV P++TVA+MGVLIQTTS +Y+FPSSL FAVSTRVGNELGA 
Sbjct: 292 VCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGAN 351

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP  AKL+A VA+  AA+ G+ A  FA  +RN W R+FT D EIL+LT+ ALPILGLCE+
Sbjct: 352 RPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEI 411

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQTVGCGV+RG+ARPSTAAN+NLGAFY VGMPVAVGL F  G+GF GLW+GLL+AQ+
Sbjct: 412 GNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQI 471

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTCTAGEELPLI 505
            CAGLM+YV+GTTDW+ +A ++  LTC    E  +I
Sbjct: 472 SCAGLMMYVVGTTDWESEAKKAQTLTCAETVENDII 507

BLAST of MC08g2527 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 616.7 bits (1589), Expect = 1.7e-176
Identity = 304/461 (65.94%), Postives = 379/461 (82.21%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E+ SEA+SLFSLAFP  L ALI+Y+RS ISMLFLG +G++ELA GSLAIAFANITGYSVL
Sbjct: 32  ELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVL 91

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           +GLALGM+PLCSQAFGA RPKLLSLTL R V+FLL SS+ I  LW+ + KI+++L QDP 
Sbjct: 92  AGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPS 151

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I+ +AQTY++ S+P+L+TNSF++P+RIYLRAQG+T PLTLA+LAG +FH+P+N  LVS+ 
Sbjct: 152 ISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYL 211

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
            +G  GV+ AAAA+N LV++FLVA+V ++G+H PTWT PS EC   W P++ LA PSC+ 
Sbjct: 212 GWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIG 271

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYEIM VLCGLL+ P   VASMG+LIQTTSL+YIFPSSLG AVSTRVGNELG+ 
Sbjct: 272 VCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSN 331

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP+KA+LSA+VAV  A ++GL A+ FA G+ + W  +FT+D  I++LT+ ALPILGLCEL
Sbjct: 332 RPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCEL 391

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQTVGCGV+RG+ARPS AANINLGAFY VG PVAVGL F    GFCGLW+GLL+AQ+
Sbjct: 392 GNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQI 451

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTCTAGEELPLISPPHN 510
           CCA +MLYV+ TTDW+ +A R+  LTCT G ++ + +   N
Sbjct: 452 CCAAMMLYVVATTDWEKEAIRARKLTCTEGVDVVITTTQTN 492

BLAST of MC08g2527 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 9.3e-143
Identity = 259/457 (56.67%), Postives = 337/457 (73.74%), Query Frame = 0

Query: 49  EIISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVL 108
           E + E K++  ++ P A+T L++YSR++ISMLFLG LG++ELA GSL+I FANITGYSV+
Sbjct: 54  EGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVI 113

Query: 109 SGLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPG 168
           SGL++GMEP+C QA+GA + KLL LTL R V+ LL  S+PIS  W+ M +ILL+  QD  
Sbjct: 114 SGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEE 173

Query: 169 ITEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHF 228
           I+ +AQ +L+F++P+L   S ++P+RIYLR Q +T+P+T ++    + HVP+N LLV   
Sbjct: 174 ISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKL 233

Query: 229 EFGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVS 288
           E GVAGVA A   TN  ++V L ++V  + VH+ TW   + + L GW  LL LA P+CVS
Sbjct: 234 EMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVS 293

Query: 289 VCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAG 348
           VCLEWWWYE MI+LCGLL  P+ATVASMG+LIQTT+L+Y+FPSSL   VSTR+ NELGA 
Sbjct: 294 VCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAK 353

Query: 349 RPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCEL 408
           RP KA++S ++++F A  LGL A +FA  +R+ W R+FT DAEIL+LTS+ALPI+GLCEL
Sbjct: 354 RPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCEL 413

Query: 409 GNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQV 468
           GNCPQT GCGVLRG ARP+  ANINLG+FY VGMPVA+   F    GF GLW GLL+AQ 
Sbjct: 414 GNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQA 473

Query: 469 CCAGLMLYVIGTTDWDFQACRSHMLTC-TAGEELPLI 505
            CA LML  +  TDW  QA R+  LT  T G+  PL+
Sbjct: 474 TCASLMLCALLRTDWKVQAERAEELTSQTPGKSPPLL 510

BLAST of MC08g2527 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 264/454 (58.15%), Postives = 337/454 (74.23%), Query Frame = 0

Query: 51  ISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIE-LAAGSLAIAFANITGYSVLS 110
           I EAKS+  ++ P+ LT L++YSRS+ISMLFLGRL D+  L+ GSLA+ FANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 111 GLALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGI 170
           GL++GMEP+C QAFGA R KLL L L R  + LL+ S+PIS+LW+ + KILLF  QD  I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 171 TEMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFE 230
           +  A+ +++FSLP+L+  SF++PIRIYLR+Q +T+PLT ++    + H+PIN LLVS   
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 231 FGVAGVAAAAAATNFLVLVFLVAYVVVSGVHAPTWTAPSRECLSGWKPLLELAAPSCVSV 290
            G+ GVA  A  TN  +L FL+ Y+V SGV+  TW   S +C  GW+ L++LA PSCVSV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 291 CLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNELGAGR 350
           CLEWWWYEIMI+LCGLL+ P+ATVASMG+LIQTT+LIYIFPSSL  +VSTRVGNELGA +
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 351 PHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILGLCELG 410
           P KA+++A   + ++  LGL A  FA  +RN WAR+FTD+ EI++LTS+ LPI+GLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 411 NCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLLSAQVC 470
           NCPQT  CGVLRGSARP   ANINL  FY VGMPVAV L+F  G  F GLWLGL +AQ  
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 471 CAGLMLYVIGTTDWDFQACRSHML---TCTAGEE 501
           C   ML V+  TDW+ +  R+  L   +C   E+
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKELMTRSCDGDED 483

BLAST of MC08g2527 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-124
Identity = 229/450 (50.89%), Postives = 311/450 (69.11%), Query Frame = 0

Query: 51  ISEAKSLFSLAFPIALTALIIYSRSIISMLFLGRLGDIELAAGSLAIAFANITGYSVLSG 110
           ++EA S+  +++P+ LT L +Y RS +S+ FLG LGD  LA GSLA AFANITGYS+ SG
Sbjct: 33  LNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYSLFSG 92

Query: 111 LALGMEPLCSQAFGAHRPKLLSLTLHRAVIFLLVSSIPISLLWVKMAKILLFLRQDPGIT 170
           L +G+E +CSQAFGA R   +  ++ R +I LLV+S+P++LLW+ M KILL L+QD  + 
Sbjct: 93  LTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQDKKLA 152

Query: 171 EMAQTYLIFSLPELVTNSFINPIRIYLRAQGVTVPLTLASLAGAVFHVPINLLLVSHFEF 230
             A  +L++S+P+LV  SF++P+R+YLR Q  T+PL++ ++  +  H+PI   LVS+   
Sbjct: 153 SEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVSYLGL 212

Query: 231 GVAGVAAAAAATNFLVLVFLVAYV------VVSGVHAPTWTAPSRECLSGWKPLLELAAP 290
           G+ G+A +   +NF ++ FL  Y+      +              + +  WK LL LA P
Sbjct: 213 GIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLCLAIP 272

Query: 291 SCVSVCLEWWWYEIMIVLCGLLVGPKATVASMGVLIQTTSLIYIFPSSLGFAVSTRVGNE 350
           SC+SVCLEWW YEIMI+LCG L+ PKA+VASMG+LIQ TSL+YIFP SL   VSTRVGNE
Sbjct: 273 SCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTRVGNE 332

Query: 351 LGAGRPHKAKLSAVVAVFVAAMLGLGATMFATGMRNKWARMFTDDAEILRLTSVALPILG 410
           LG+ +P +A+ +A+V + ++  LG  A  F   +RN WA  FTDD EI++LT++ALPI+G
Sbjct: 333 LGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMALPIVG 392

Query: 411 LCELGNCPQTVGCGVLRGSARPSTAANINLGAFYGVGMPVAVGLAFRLGVGFCGLWLGLL 470
           LCELGNCPQT GCGVLRGSARP   ANIN  AFY VG+PV   LAF  G GF GLWLG+L
Sbjct: 393 LCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLWLGML 452

Query: 471 SAQVCCAGLMLYVIGTTDWDFQACRSHMLT 495
           +AQ+ C   M+     TDW+ +A R+ +LT
Sbjct: 453 AAQITCVIGMMAATCRTDWELEAERAKVLT 482