MC08g0936 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0936
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionphosphatidyl-N-methylethanolamine N-methyltransferase
LocationMC08: 7509657 .. 7512427 (+)
RNA-Seq ExpressionMC08g0936
SyntenyMC08g0936
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGGATCATTTTCCCGAGAAAGTGGAGGAAATTCCGGTGAGAAAAATATCAGAATCAGAATCAGAATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATCTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGATGTGCTGAAATCTGCCTTCAATCTGTATAGTAGCCTACTTGACTTAACTTTTGTGCTACTGCTTGCTATATTGATGAGAGGAAATTTACGAGCTGGATATGTGGAAGTAACGATTTATTAATGATCTGAAAAGCGTCTGAAAAGCGATCCTACCTGTGAATTTAATGATTAAATTTCTCTTTACTTCTTAAGATTACTTCATTAGGAGATTATTAGCATTTAATAAAGGTTAGAATCATTGGCAATCTCTTCTTTATCAGGGAGAGAGAGAGACGAAATTTGATTTCAGTTTCTATCTTCAAGTTTAGCTATCGCTGATCGTGATCAATCTCTTCATTCTCCAATTGACCATTCAGTTGATGTATATGTGGAAGGTTTTTTTTTCCATGTACTTTTTGGGTAAGAAATAGTTGTTGCACCAAAGTAGGTCCTAGAAAGTGAAATACTTGGAGTTTGGTCTTCGCTACAAGGTTTTATACATCAAGCGGTCGTGGCTCTGAAGTTCTTTTGGTTTTTCAAGCTTAGTAGGAAGCAGTTTTCTGAGACCTGGTTTAGAATGCTTTTTTATCTTTTTTGAGTTTGTACTCTTGTTGTGCTAATTCTGCTTTATTTTTCTCTTCTTCTCTCTCTTATTTTTTCACCCTTTCGGAGTCCTCTATCTTGAACATTTTTCCTTTTCATATATTGATGAAAAAAAATTTTTTTTCCAAAAGAAAGGAAGTGAATACTTGGTGTGACCGACTTATCTGAGGCTGCTGCAGTGTCCCGAGTACTGTTTTTCATTTCTATTAAGGATTACTGTATTGTGCTTCCCAGTGCTCACATCCTTGGTTATCACTTTTATAAGAATTCAACTTGGTTCTGTCTTTCCCAAAGAATGCTTCCCCTCTAGCCTTGAACAACTTTATAAAGTTACCTTTGAGAAAAGTGGTGTCGAGGGGATTTTCCTGAAATTTGACTTCGTAGAAGTTACAGACTTTTCAAGAATACTTCTCGAGGAAGTGAAAAAAAACTTAGAAGTTGTGCGTTTTATAGTACTTCTATAAAGTTTCCTATCTTCAAAATCTTTTTGTAACTATGTACATATTTTTTTATTAATGCTATTCTAGTGGACTGTACTTAGTCTGCTAGTTTCGTTTAAGTTGATGCCTCAACCACCCAATTTATTTCTCTTGGTGTTAATGGAAGTTTGTTTCCTATTAAAAAGAAGAAAGAAATTGATGTGGATAGGTTTATATCAAGATTTTCCCCTATCATAATGCAACAGTAAAAAGTGAAAGTGTGGAGATTGTTGCGCCAAAGTGAGCACATAAATTTAGAAATCAATAGTTGCAAGGGCCATTTTCTTGCCTCGGATTCTGTGTACTTTAATGAGATGAGAATGATTGCAATCTAAGGAATTGGCTTTATCTGCTCTTGGGTTGAGGAATGGAATTTTTAATATGAATAAGAATCTATCATAGATGTCCTTTCGTTGGTTTGCAATATCCAAGCAATTACTAAAATATATGTTCAAATTCATTATGTGAACTTTATCTGCTCTTGGATACTCTTTCATTGATTTCAGAGATGAAAAATGATGCTTGCAATGGTCAAACAAGCTTTAGAGACAGCATTTTCCCAGTATAATTGCCTCATATGATTGGTAGTCTTCACAAGATAGATCTTTTGTGATTATTACTATATAACAAAGTAAAAACAACTAAGATTACATTAATGTTCGTTTCTTATGCTCAAGCTTGAAAATTCAGAAACAAGCGTAGAATAAGCATCTCAGGCTGCTAGTGTTTTAATTAAAAGCAAAGAAACTATAATCAAGATTTTCGAGTTTAGGATGTCCTATCCTAAATTTTTAGGCCAATTTAAGGATACATAAGTGATCTCATGGGACAACTGTCCCTGTCTAACCTAGTAGGGTAGATTTTAATATCTTAAGTTGGTGGCCACATGAGTTTCAACCCATTCTCTCAAAGTCTATATATTTTTTTGACCCACCTCTATAACCATCAGGCCAATCCGGACAGGGTCAAGTACGATTAGATGCATGAGACTATTGGACCTCAATTTTTTACAATGTTGCTGCAGTTTTCCAGACTCGATTGAATGAAAACAATATCGTGTTGCTCCGGCTTGTATTATAAAGTAATTCTTTCTTCTGTCTTCTGCCAAATTATAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTGTTGACGCTGGGATACGTCTTTATGATGCAAGTTGAATCAAAGGAAGATCCCACAACTCGGGCAAAGCCTCAAAGT

mRNA sequence

GGGGATCATTTTCCCGAGAAAGTGGAGGAATCCGGTGAGAAAAATATCAGAATCAGAATCAGAATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATCTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTGTTGACGCTGGGATACGTCTTTATGATGCAAGTTGAATCAAAGGAAGATCCCACAACTCGGGCAAAGCCTCAAAGT

Coding sequence (CDS)

GGGGATCATTTTCCCGAGAAAGTGGAGGAATCCGGTGAGAAAAATATCAGAATCAGAATCAGAATGGGCATAATCGCAGGAATTGGAGTGCTGCTGCCATTTCCCTTCTACTACGTCTTATGGACTCGTCCACAGTTATGGGCGAATTTTTGCGGGAAATGGCGCGAACCCTGCAAATTGATGGCTCGGGTCTCCATCTTTCTCAAGTTGCTTCAGTTCCTCTCCATCCTCCACGTCTCCTCCTTCTCATGGCCGCCGCCTCTCTACTTCTGGCCCCTCATGGCCTTTGGCCAATTTTTGAACTTCAGGGTCTATCAGTTACTGGGTGAATCCGGTGTTTACTATGGAGTACGGTTTGGGAAGAACATTCCATGGGTGACAGAATTTCCATTTGGATATATAAGAGATCCTCAGTATGTGGGAAGCATTTTGAGCATTTTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTGTTGACGCTGGGATACGTCTTTATGATGCAAGTTGAATCAAAGGAAGATCCCACAACTCGGGCAAAGCCTCAAAGT

Protein sequence

GDHFPEKVEESGEKNIRIRIRMGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVGSILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS
Homology
BLAST of MC08g0936 vs. ExPASy Swiss-Prot
Match: Q9SAH5 (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=PLMT PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 3.2e-69
Identity = 122/161 (75.78%), Postives = 135/161 (83.85%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAK 183
           SI+S+ ACLSWVP QYILL +LGYVFMM +ESKEDP  RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

BLAST of MC08g0936 vs. NCBI nr
Match: XP_022153398.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Momordica charantia])

HSP 1 Score: 347 bits (890), Expect = 2.17e-120
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS
Sbjct: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS
Sbjct: 121 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 164

BLAST of MC08g0936 vs. NCBI nr
Match: XP_008465151.2 (PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo])

HSP 1 Score: 318 bits (814), Expect = 8.41e-109
Identity = 147/164 (89.63%), Postives = 156/164 (95.12%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+FACLSWVPL+YILL TLGY+FMM VESKEDP TRAKPQS
Sbjct: 121 SILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDPATRAKPQS 164

BLAST of MC08g0936 vs. NCBI nr
Match: KAG7036543.1 (Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313 bits (803), Expect = 4.00e-107
Identity = 146/164 (89.02%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+ ACLSWVPLQYILL T+GYVFMMQVESKED TTRAKPQS
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of MC08g0936 vs. NCBI nr
Match: XP_004137657.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650956.1 hypothetical protein Csa_002414 [Cucumis sativus])

HSP 1 Score: 313 bits (802), Expect = 5.68e-107
Identity = 146/164 (89.02%), Postives = 154/164 (93.90%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+FACLSWVPL+YILL TLGY+FMM VESKED  TRAKPQS
Sbjct: 121 SILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS 164

BLAST of MC08g0936 vs. NCBI nr
Match: XP_023523751.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312 bits (800), Expect = 1.15e-106
Identity = 145/164 (88.41%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+ ACLSWVPLQYILL T+GYVFMMQVESKED TTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164

BLAST of MC08g0936 vs. ExPASy TrEMBL
Match: A0A6J1DHC8 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020909 PE=4 SV=1)

HSP 1 Score: 347 bits (890), Expect = 1.05e-120
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS
Sbjct: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS
Sbjct: 121 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 164

BLAST of MC08g0936 vs. ExPASy TrEMBL
Match: A0A1S3CPQ5 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103502819 PE=4 SV=1)

HSP 1 Score: 318 bits (814), Expect = 4.07e-109
Identity = 147/164 (89.63%), Postives = 156/164 (95.12%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MGI+A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSS
Sbjct: 1   MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+FACLSWVPL+YILL TLGY+FMM VESKEDP TRAKPQS
Sbjct: 121 SILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDPATRAKPQS 164

BLAST of MC08g0936 vs. ExPASy TrEMBL
Match: A0A6J1KB80 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492722 PE=4 SV=1)

HSP 1 Score: 312 bits (800), Expect = 5.55e-107
Identity = 145/164 (88.41%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           M ++A IGVLLPFP+YY+LWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYLLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+ ACLSWVPLQYILL T+GYVFMMQVESKED TTRAKPQS
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of MC08g0936 vs. ExPASy TrEMBL
Match: A0A6J1GCG7 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452701 PE=4 SV=1)

HSP 1 Score: 311 bits (797), Expect = 1.59e-106
Identity = 145/164 (88.41%), Postives = 154/164 (93.90%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQ LSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQILSILHVSS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+ ACLSWVPLQYILL T+GYVFMMQVESKED TTRAKPQS
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of MC08g0936 vs. ExPASy TrEMBL
Match: A0A1U8H4P5 (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Capsicum annuum OX=4072 GN=LOC107876013 PE=3 SV=1)

HSP 1 Score: 281 bits (719), Expect = 1.23e-94
Identity = 128/164 (78.05%), Postives = 143/164 (87.20%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MG++A +GV+LPFPFYY LWTRPQ W + CG+ R+PCK+MA  S F+KLLQF+S+L VS+
Sbjct: 1   MGLLACLGVILPFPFYYYLWTRPQTWVDLCGRGRDPCKVMAMASHFIKLLQFISLLSVST 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
           FSWPPPLYFWPL  FGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  FSWPPPLYFWPLFIFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 185
           SILS+ ACLSWVP  YILL  LGYVFM+QVESKEDP TRAKP S
Sbjct: 121 SILSLLACLSWVPFFYILLWVLGYVFMIQVESKEDPATRAKPLS 164

BLAST of MC08g0936 vs. TAIR 10
Match: AT1G80860.2 (phospholipid N-methyltransferase )

HSP 1 Score: 262.7 bits (670), Expect = 2.2e-70
Identity = 122/161 (75.78%), Postives = 135/161 (83.85%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAK 183
           SI+S+ ACLSWVP QYILL +LGYVFMM +ESKEDP  RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

BLAST of MC08g0936 vs. TAIR 10
Match: AT1G80860.1 (phospholipid N-methyltransferase )

HSP 1 Score: 262.7 bits (670), Expect = 2.2e-70
Identity = 122/161 (75.78%), Postives = 135/161 (83.85%), Query Frame = 0

Query: 22  MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 81
           MG++A IGVLLPFPFY+ LWT  Q W N CG+ R+P  +MARVS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 82  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 141
            SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGKNIPWVTEFPFG IRDPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 142 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAK 183
           SI+S+ ACLSWVP QYILL +LGYVFMM +ESKEDP  RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAH53.2e-6975.78Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_022153398.12.17e-120100.00phosphatidyl-N-methylethanolamine N-methyltransferase [Momordica charantia][more]
XP_008465151.28.41e-10989.63PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo][more]
KAG7036543.14.00e-10789.02Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma su... [more]
XP_004137657.15.68e-10789.02phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650... [more]
XP_023523751.11.15e-10688.41phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
A0A6J1DHC81.05e-120100.00phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=... [more]
A0A1S3CPQ54.07e-10989.63phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN... [more]
A0A6J1KB805.55e-10788.41phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=366... [more]
A0A6J1GCG71.59e-10688.41phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3... [more]
A0A1U8H4P51.23e-9478.05Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Capsicum annuum OX=4072... [more]
Match NameE-valueIdentityDescription
AT1G80860.22.2e-7075.78phospholipid N-methyltransferase [more]
AT1G80860.12.2e-7075.78phospholipid N-methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007318Phospholipid methyltransferasePFAMPF04191PEMTcoord: 92..179
e-value: 6.5E-12
score: 45.8
NoneNo IPR availableGENE3D1.20.120.1630coord: 41..182
e-value: 1.6E-6
score: 29.9
NoneNo IPR availablePANTHERPTHR15458:SF5PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASEcoord: 31..183
IPR024960Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferasePANTHERPTHR15458PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASEcoord: 31..183
IPR024960Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferaseHAMAPMF_03216PLMTcoord: 20..185
score: 15.678885

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0936.1MC08g0936.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006656 phosphatidylcholine biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004608 phosphatidylethanolamine N-methyltransferase activity
molecular_function GO:0080101 phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
molecular_function GO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity