MC06g2254 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g2254 vs. ExPASy Swiss-Prot
Match: D9IWE0 (Antifungal protein ginkbilobin-like protein OS=Pinus pinaster OX=71647 GN=EAP PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 8.8e-13
Identity = 49/125 (39.20%), Postives = 59/125 (47.20%), Query Frame = 0

Query: 16  FGLSTMVVCV--------PNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKY 75
           F L+ MVV +        P T + S  CN      G  F  +L  +L +L  +T SS  Y
Sbjct: 7   FTLAVMVVALVVGSAVGTPRTNLVSSACNGQKIQSGSSFYSTLGSLLVDLEGNTASS-GY 66

Query: 76  DFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRY 133
           D Y  S   +  AYG   CNQ L+ SDCT CL      +   C   IG R  L DC IRY
Sbjct: 67  D-YKASRAGSPTAYGRAVCNQGLSQSDCTACLKNLGGRIWNICGYAIGARVQLTDCFIRY 126

BLAST of MC06g2254 vs. ExPASy Swiss-Prot
Match: A9NQT1 (Antifungal protein ginkbilobin-like protein 1 OS=Picea sitchensis OX=3332 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-11
Identity = 45/124 (36.29%), Postives = 57/124 (45.97%), Query Frame = 0

Query: 16  FGLSTMVVCV--------PNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTP-SSKK 75
           F L+ MV+ V        PNT   S  CN+     G+PF  +L  +L  L  +T  S   
Sbjct: 8   FALAVMVLAVLVASAAGAPNTNFVSSACNTKKIPSGNPFFNNLGALLVNLEKNTAFSGYD 67

Query: 76  YDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIR 131
           Y            AYG   C Q+++ SDCT CL      + G C+  IG R  L DC IR
Sbjct: 68  YKASRAGSGGAPTAYGRGVCKQSISQSDCTACLTNLGGRIWGICKNAIGARVQLTDCFIR 127

BLAST of MC06g2254 vs. ExPASy Swiss-Prot
Match: A4ZDL6 (Antifungal protein ginkbilobin-2 OS=Ginkgo biloba OX=3311 GN=GNK2 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-10
Identity = 41/132 (31.06%), Postives = 64/132 (48.48%), Query Frame = 0

Query: 5   SLKISAAFLAFFGLSTMVVCV---PNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNST 64
           ++++++AF+  F L+  ++ +    NT   S  CN+     G PF  +L  +L +L  +T
Sbjct: 3   TMRMNSAFILAFALAAAMLILTEAANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNT 62

Query: 65  P-SSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 124
             S   Y            AYG  +C Q+++ SDCT CL    + +   C   IG R  L
Sbjct: 63  AFSGYDYKTSRAGSGGAPTAYGRATCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQL 122

Query: 125 NDCAIRYEQYPF 133
            DC I+YEQ  F
Sbjct: 123 VDCFIQYEQRSF 134

BLAST of MC06g2254 vs. ExPASy Swiss-Prot
Match: Q40858 (Antifungal protein ginkbilobin-like protein 2 OS=Picea glauca OX=3330 GN=EMB35 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-10
Identity = 44/124 (35.48%), Postives = 57/124 (45.97%), Query Frame = 0

Query: 16  FGLSTMVVCV--------PNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTP-SSKK 75
           F L+ MV+ V        PNT + S  CN      G+PF  +L  +L +L  +T  S   
Sbjct: 8   FALAVMVLAVLVASAAGAPNTNLVSSACNGNKIPSGNPFFNNLGALLVDLEKNTAFSGFD 67

Query: 76  YDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIR 131
           Y            AYG   C Q+++ SDCT CL      + G  +  IG R  L DC IR
Sbjct: 68  YKASRAGSGGAPTAYGRGVCKQSISQSDCTACLTNLGGRIWGISKNAIGARVQLTDCFIR 127

BLAST of MC06g2254 vs. ExPASy Swiss-Prot
Match: D5A8I3 (Antifungal protein ginkbilobin-like protein 2 OS=Picea sitchensis OX=3332 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-09
Identity = 35/107 (32.71%), Postives = 51/107 (47.66%), Query Frame = 0

Query: 27  NTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTP-SSKKYDFYNISPYPNAFAYGHVS 86
           NT   S  CN+     G PF  +L   +E+L  +T      Y   ++       AYG   
Sbjct: 25  NTKFVSASCNTEKIARGSPFFNNLRDAIEDLRQNTAYIGYDYKTSHVGSGGAPTAYGRAI 84

Query: 87  CNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYPF 133
           C Q+++ S+CT+CL    + + G C   IG R  L DC ++YEQ  F
Sbjct: 85  CKQSISQSNCTSCLSNVATRIYGICGNAIGARVQLTDCYMQYEQRSF 131

BLAST of MC06g2254 vs. NCBI nr
Match: KGN47889.1 (hypothetical protein Csa_003100 [Cucumis sativus])

HSP 1 Score: 233 bits (593), Expect = 1.90e-76
Identity = 110/134 (82.09%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MDGSSLKISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNS 60
           M GSSLKIS AFLAFF LS MVV +PNTGV+SVLCNSG FTGGDPF VSL YVL+EL ++
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPSSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 120
           TP+ K YDFYNISPYPNAFAYGH SCNQNL+TSDCTTCL AAK+NMLG+CQMRIGGR+VL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 NDCAIRYEQYPFDD 134
           NDC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of MC06g2254 vs. NCBI nr
Match: KAG6606427.1 (Antifungal protein ginkbilobin-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036367.1 Antifungal protein ginkbilobin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 231 bits (590), Expect = 5.45e-76
Identity = 111/134 (82.84%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MDGSSLKISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNS 60
           M G SLKISAAFLA FG   MVV VPNTGVKSVLCNSGIFTGGDPFAVSL YVL+EL +S
Sbjct: 1   MVGCSLKISAAFLALFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESS 60

Query: 61  TPSSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 120
           TP++  YDFYNISPYPN+FAYGH SCNQ+LTTSDCTTCLGAAK+NMLG+CQMRIG RAVL
Sbjct: 61  TPTTNNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVL 120

Query: 121 NDCAIRYEQYPFDD 134
           NDC+IRYEQ PFDD
Sbjct: 121 NDCSIRYEQNPFDD 134

BLAST of MC06g2254 vs. NCBI nr
Match: KAA0057296.1 (antifungal protein ginkbilobin-2-like [Cucumis melo var. makuwa] >TYK13437.1 antifungal protein ginkbilobin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 229 bits (584), Expect = 4.48e-75
Identity = 108/134 (80.60%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MDGSSLKISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNS 60
           M GSSLKIS AFLAFF +S MVV +PNTGV+SVLCNSG FTGGDPFAVSL YVL+EL ++
Sbjct: 1   MIGSSLKISVAFLAFFAVSIMVVSIPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELQSA 60

Query: 61  TPSSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 120
           TP+ K YDFYNISPYPNAFAYGH SCNQNLTTSDCT+CL AA++NMLG+CQ RIGGR+VL
Sbjct: 61  TPTIKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTSCLEAAENNMLGSCQKRIGGRSVL 120

Query: 121 NDCAIRYEQYPFDD 134
           NDC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of MC06g2254 vs. NCBI nr
Match: XP_038887019.1 (antifungal protein ginkbilobin-like protein [Benincasa hispida])

HSP 1 Score: 210 bits (535), Expect = 6.88e-68
Identity = 98/114 (85.96%), Postives = 105/114 (92.11%), Query Frame = 0

Query: 21  MVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKYDFYNISPYPNAFA 80
           MVV VPNTGV+SVLCNSG FTGGDPFAVSL YVL+EL +STP+ KKYDFYNISPYPN+FA
Sbjct: 1   MVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKYDFYNISPYPNSFA 60

Query: 81  YGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYPFDD 134
           YGH SCNQNLTTSDCT CLGAAK+NMLGTCQM IGGRAVL+DC IRYEQYPFDD
Sbjct: 61  YGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPFDD 114

BLAST of MC06g2254 vs. NCBI nr
Match: XP_022931011.1 (antifungal protein ginkbilobin-2-like [Cucurbita moschata])

HSP 1 Score: 208 bits (530), Expect = 3.98e-67
Identity = 97/114 (85.09%), Postives = 106/114 (92.98%), Query Frame = 0

Query: 21  MVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKYDFYNISPYPNAFA 80
           MVV VPNTGVKSVLCNSGIFTGGDPFAVSL YVL+EL +STP++  YDFYNISPYPN+FA
Sbjct: 1   MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA 60

Query: 81  YGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYPFDD 134
           YGH SCNQ+LTTSDCTTCLGAAK+NMLG+CQMRIG RAVLNDC+IRYEQ PFDD
Sbjct: 61  YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD 114

BLAST of MC06g2254 vs. ExPASy TrEMBL
Match: A0A0A0KGA2 (Gnk2-homologous domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G409950 PE=4 SV=1)

HSP 1 Score: 233 bits (593), Expect = 9.21e-77
Identity = 110/134 (82.09%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MDGSSLKISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNS 60
           M GSSLKIS AFLAFF LS MVV +PNTGV+SVLCNSG FTGGDPF VSL YVL+EL ++
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPSSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 120
           TP+ K YDFYNISPYPNAFAYGH SCNQNL+TSDCTTCL AAK+NMLG+CQMRIGGR+VL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 NDCAIRYEQYPFDD 134
           NDC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of MC06g2254 vs. ExPASy TrEMBL
Match: A0A5D3CNH9 (Antifungal protein ginkbilobin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00580 PE=4 SV=1)

HSP 1 Score: 229 bits (584), Expect = 2.17e-75
Identity = 108/134 (80.60%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MDGSSLKISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNS 60
           M GSSLKIS AFLAFF +S MVV +PNTGV+SVLCNSG FTGGDPFAVSL YVL+EL ++
Sbjct: 1   MIGSSLKISVAFLAFFAVSIMVVSIPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELQSA 60

Query: 61  TPSSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVL 120
           TP+ K YDFYNISPYPNAFAYGH SCNQNLTTSDCT+CL AA++NMLG+CQ RIGGR+VL
Sbjct: 61  TPTIKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTSCLEAAENNMLGSCQKRIGGRSVL 120

Query: 121 NDCAIRYEQYPFDD 134
           NDC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of MC06g2254 vs. ExPASy TrEMBL
Match: A0A6J1ESI5 (antifungal protein ginkbilobin-2-like OS=Cucurbita moschata OX=3662 GN=LOC111437337 PE=4 SV=1)

HSP 1 Score: 208 bits (530), Expect = 1.93e-67
Identity = 97/114 (85.09%), Postives = 106/114 (92.98%), Query Frame = 0

Query: 21  MVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKYDFYNISPYPNAFA 80
           MVV VPNTGVKSVLCNSGIFTGGDPFAVSL YVL+EL +STP++  YDFYNISPYPN+FA
Sbjct: 1   MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA 60

Query: 81  YGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYPFDD 134
           YGH SCNQ+LTTSDCTTCLGAAK+NMLG+CQMRIG RAVLNDC+IRYEQ PFDD
Sbjct: 61  YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD 114

BLAST of MC06g2254 vs. ExPASy TrEMBL
Match: A0A5E4EE49 (PREDICTED: antifungal OS=Prunus dulcis OX=3755 GN=ALMOND_2B017312 PE=4 SV=1)

HSP 1 Score: 190 bits (482), Expect = 7.86e-60
Identity = 88/123 (71.54%), Postives = 103/123 (83.74%), Query Frame = 0

Query: 12  FLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKYDFYN 71
           F+ FFG++T     P+T + SVLCNSG++T GDPFAVSL+YVL+EL  +T + K YDFY+
Sbjct: 16  FIGFFGVATST---PDTNITSVLCNSGLYTAGDPFAVSLAYVLKELETATSTHKNYDFYD 75

Query: 72  ISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYP 131
           ISPYPNAFAYGH SCNQN+TT DCTTCLGAAK++MLGTCQ  IGGRAVL DCAIRYEQYP
Sbjct: 76  ISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRYEQYP 135

Query: 132 FDD 134
           F D
Sbjct: 136 FTD 135

BLAST of MC06g2254 vs. ExPASy TrEMBL
Match: A0A6J5YEQ2 (Gnk2-homologous domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS52084 PE=4 SV=1)

HSP 1 Score: 189 bits (481), Expect = 1.15e-59
Identity = 89/127 (70.08%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 8   ISAAFLAFFGLSTMVVCVPNTGVKSVLCNSGIFTGGDPFAVSLSYVLEELGNSTPSSKKY 67
           I   FL FF ++T     P+T + SVLCNSG++T GDPFAVSL+YVL+EL  +T + K Y
Sbjct: 13  IVVVFLGFFSVATST---PDTNITSVLCNSGVYTAGDPFAVSLAYVLQELETATSTHKNY 72

Query: 68  DFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRY 127
           DFY+ISPYPNAFAYGH SCNQN+TT DCTTCLGAAK++MLGTCQ  IGGRAVL DCAIRY
Sbjct: 73  DFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRY 132

Query: 128 EQYPFDD 134
           EQYPF D
Sbjct: 133 EQYPFTD 136

BLAST of MC06g2254 vs. TAIR 10
Match: AT5G48540.1 (receptor-like protein kinase-related family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 0

Query: 82  GHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLNDCAIRYEQYPF 133
           G V C ++L+  DC  C   A  + + TC  + G R + + C +RYE YPF
Sbjct: 190 GLVQCTRDLSELDCAQCFATAVGSFMTTCHNKKGCRVLYSSCYVRYEFYPF 240

BLAST of MC06g2254 vs. TAIR 10
Match: AT1G70690.1 (Receptor-like protein kinase-related family protein )

HSP 1 Score: 44.3 bits (103), Expect = 9.1e-05
Identity = 29/134 (21.64%), Postives = 57/134 (42.54%), Query Frame = 0

Query: 6   LKISAAFLAFFGLSTMVVCVPNTGVKS-----VLCNSGIFTGGDPFAVSLSYVLEELGNS 65
           +K     L  F L+ +++  P++   +      +C+   F+    +  +L+ +L     S
Sbjct: 2   IKTKTTSLLCFLLTAVILMNPSSSSPTDNYIYAVCSPAKFSPSSGYETNLNSLLSSFVTS 61

Query: 66  TPSSKKYDFY--NISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRA 125
           T  ++  +F      P P    YG   C  +L  + C+TC+ +A + +   C     G  
Sbjct: 62  TAQTRYANFTVPTGKPEPTVTVYGIYQCRGDLDPTACSTCVSSAVAQVGALCSNSYSGFL 121

Query: 126 VLNDCAIRYEQYPF 133
            + +C IRY+   F
Sbjct: 122 QMENCLIRYDNKSF 135

BLAST of MC06g2254 vs. TAIR 10
Match: AT4G23270.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 19 )

HSP 1 Score: 41.6 bits (96), Expect = 5.9e-04
Identity = 25/70 (35.71%), Postives = 32/70 (45.71%), Query Frame = 0

Query: 63  SSKKYDFYNISPYPNAFAYGHVSCNQNLTTSDCTTCLGAAKSNMLGTCQMRIGGRAVLND 122
           S KK+    I    +   YG V C  +L   DC  CL   K  +      +IGGR +L  
Sbjct: 173 SFKKWAVRKIDLNASQSLYGMVRCTPDLREQDCLDCL---KIGINQVTYDKIGGRILLPS 232

Query: 123 CAIRYEQYPF 133
           CA RY+ Y F
Sbjct: 233 CASRYDNYAF 239