MC06g2133 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g2133
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionbifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase
LocationMC06: 28794441 .. 28796327 (-)
RNA-Seq ExpressionMC06g2133
SyntenyMC06g2133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGGATCTGAAGCACTCTCCAAACTTCCATTTCCTTTGAATCTCTCTTCTTCTTCTTCTTCAATCATCTGTCAATCCTCTTCCTTCTGCGATGGGTATTCTCGCTGACGGTGGGTCAGCTCCGGATGCGTCTTTCAGGTTTTTGATATACGGCCGGACCGGCTGGATTGGCGGTTTGCTCGGCAAGCTCTGCGAACAACAGGGAATCGATTTTTCTTATGGGTCCGGTCGCCTCGAGAACCGCGCCTCCCTCGAGGCCGACATCGCCGCCGTGAAGCCTACCCACGTCTTCAACGCCGCCGGTGTAACCGGCCGCCCCAACGTCGACTGGTGCGAGTCGCATAAGGTCGAGACCATTAGAACCAATGTGGTCGGAACCCTGAGCTTGGCCGATGTGTGCCGCGAGAGAGGGTTGGTTCTCATTAATTACGCCACCGGCTGCATTTTCGAGTACGATTCCGCGCACCCTCTCAATTCCGGGATCGGCTTCAAGGAGGAAGAAACCCCCAATTTTATTGGATCCTTCTATTCCAAAACCAAAGCCATGGTGAGCCACAGCCTCCGATTTTTTTAACTGCTCCCTTTTGATTATTGCGTGATTTATATTGGATAAACATTGTGGTGCTGTTGGAATTTCAGTTATAGTCTAATTTCATTACAGATCTATAGTAATAATGTCAATGGTTCTATTTAAAGAAATCACAATTGAAATCTGCCATCAATGGTTAGCAGGTCCTTCTGAGGGTGGATTGGATATTACTTTGTTTGTGAATGCTCCGATCTAGAGTGTTATTACATTCATTAGAATAGCAGTTTAGGAGGTTAAATCCAAGGCATTGTTATAGTTTGTGTTGAAGATTCTGATTATTGATTGCTATTTGAAGGAAGAATATCAAATTTGTTTCTATTGACCCTGATGCCTGATGCCTGATGGGCTGATTCTGTTTTTATGTCACTTTCACATTTTGGGGGTCAAAAAGGTGGAGGATCTTCTGAAGAACTATGAGAATGTGTGCACCTTGCGAGTTCGGATGCCTATATCCTCCGACTTATCGAACCCTCGCAACTTCATTACAAAGATTACCAAATACGAGAAGGTGGTTGACATCCCGAACTCGATGACGATCTTGGACGAACTCCTCCCGATCTCGATCGAGATGGCAAAAAGAAACCTCACGGGGATATGGAACTTCACCAACCCGGGAGTGGTGAGCCACAACGAGATCTTGGAGATGTACAAGCAATTCATTGACCCCAATTTCACATGGAAGAACTTCAATCTGGAGGAGCAAGCCAAAGTGATTGTCGCCCCGAGGAGCAACAACGAGCTTGACGCGTCCAAGTTGAAGAACGAATTCCCCGAACTTATGTCCATCAAGGACTCTCTCATCAAGTATGTCTTCAAGCCAAATCAGAAAACTTCTGCAGCTTAAAATCCTTGGTACTTCTTTTGTATTTTCCTTTGCCATTTCAGTTCATTTGAATCTTTGGCGAGTTCTCGAATATCAGATACTTTGCCTTCTCCCACCCAACATAGGAACTTGATTATTATCCATATTTGAATGCCATATCTATTATTTTGTTCCTATCATTATTGAATAAAGTAGCTTGAAAAATGATGTGGGGCAATAATATAGGCCATTTTTAGCAGGATGAAGAAGTGGCATTTTGGTATTTGTCGCTTATTTGGGTATAAGATATTACTGAGAACTTCTGAGCTTCATCAGTAATGAATTCTGTTTTTAAGGTTGAAGTTAATCTTTAATGTAGTTATCTTTGGGTGTTTAGCAAGGTTTCTGTGTATTGTTTTTTTAGAAACATTATCAATATCATGTAGAAATTTTGGAAACTTTTTTCGTTTCTAACTTATTCCAAATTCTTGAATCT

mRNA sequence

CTCGGATCTGAAGCACTCTCCAAACTTCCATTTCCTTTGAATCTCTCTTCTTCTTCTTCTTCAATCATCTGTCAATCCTCTTCCTTCTGCGATGGGTATTCTCGCTGACGGTGGGTCAGCTCCGGATGCGTCTTTCAGGTTTTTGATATACGGCCGGACCGGCTGGATTGGCGGTTTGCTCGGCAAGCTCTGCGAACAACAGGGAATCGATTTTTCTTATGGGTCCGGTCGCCTCGAGAACCGCGCCTCCCTCGAGGCCGACATCGCCGCCGTGAAGCCTACCCACGTCTTCAACGCCGCCGGTGTAACCGGCCGCCCCAACGTCGACTGGTGCGAGTCGCATAAGGTCGAGACCATTAGAACCAATGTGGTCGGAACCCTGAGCTTGGCCGATGTGTGCCGCGAGAGAGGGTTGGTTCTCATTAATTACGCCACCGGCTGCATTTTCGAGTACGATTCCGCGCACCCTCTCAATTCCGGGATCGGCTTCAAGGAGGAAGAAACCCCCAATTTTATTGGATCCTTCTATTCCAAAACCAAAGCCATGGTGGAGGATCTTCTGAAGAACTATGAGAATGTGTGCACCTTGCGAGTTCGGATGCCTATATCCTCCGACTTATCGAACCCTCGCAACTTCATTACAAAGATTACCAAATACGAGAAGGTGGTTGACATCCCGAACTCGATGACGATCTTGGACGAACTCCTCCCGATCTCGATCGAGATGGCAAAAAGAAACCTCACGGGGATATGGAACTTCACCAACCCGGGAGTGGTGAGCCACAACGAGATCTTGGAGATGTACAAGCAATTCATTGACCCCAATTTCACATGGAAGAACTTCAATCTGGAGGAGCAAGCCAAAGTGATTGTCGCCCCGAGGAGCAACAACGAGCTTGACGCGTCCAAGTTGAAGAACGAATTCCCCGAACTTATGTCCATCAAGGACTCTCTCATCAAGTATGTCTTCAAGCCAAATCAGAAAACTTCTGCAGCTTAAAATCCTTGGTACTTCTTTTGTATTTTCCTTTGCCATTTCAGTTCATTTGAATCTTTGGCGAGTTCTCGAATATCAGATACTTTGCCTTCTCCCACCCAACATAGGAACTTGATTATTATCCATATTTGAATGCCATATCTATTATTTTGTTCCTATCATTATTGAATAAAGTAGCTTGAAAAATGATGTGGGGCAATAATATAGGCCATTTTTAGCAGGATGAAGAAGTGGCATTTTGGTATTTGTCGCTTATTTGGGTATAAGATATTACTGAGAACTTCTGAGCTTCATCAGTAATGAATTCTGTTTTTAAGGTTGAAGTTAATCTTTAATGTAGTTATCTTTGGGTGTTTAGCAAGGTTTCTGTGTATTGTTTTTTTAGAAACATTATCAATATCATGTAGAAATTTTGGAAACTTTTTTCGTTTCTAACTTATTCCAAATTCTTGAATCT

Coding sequence (CDS)

ATGGGTATTCTCGCTGACGGTGGGTCAGCTCCGGATGCGTCTTTCAGGTTTTTGATATACGGCCGGACCGGCTGGATTGGCGGTTTGCTCGGCAAGCTCTGCGAACAACAGGGAATCGATTTTTCTTATGGGTCCGGTCGCCTCGAGAACCGCGCCTCCCTCGAGGCCGACATCGCCGCCGTGAAGCCTACCCACGTCTTCAACGCCGCCGGTGTAACCGGCCGCCCCAACGTCGACTGGTGCGAGTCGCATAAGGTCGAGACCATTAGAACCAATGTGGTCGGAACCCTGAGCTTGGCCGATGTGTGCCGCGAGAGAGGGTTGGTTCTCATTAATTACGCCACCGGCTGCATTTTCGAGTACGATTCCGCGCACCCTCTCAATTCCGGGATCGGCTTCAAGGAGGAAGAAACCCCCAATTTTATTGGATCCTTCTATTCCAAAACCAAAGCCATGGTGGAGGATCTTCTGAAGAACTATGAGAATGTGTGCACCTTGCGAGTTCGGATGCCTATATCCTCCGACTTATCGAACCCTCGCAACTTCATTACAAAGATTACCAAATACGAGAAGGTGGTTGACATCCCGAACTCGATGACGATCTTGGACGAACTCCTCCCGATCTCGATCGAGATGGCAAAAAGAAACCTCACGGGGATATGGAACTTCACCAACCCGGGAGTGGTGAGCCACAACGAGATCTTGGAGATGTACAAGCAATTCATTGACCCCAATTTCACATGGAAGAACTTCAATCTGGAGGAGCAAGCCAAAGTGATTGTCGCCCCGAGGAGCAACAACGAGCTTGACGCGTCCAAGTTGAAGAACGAATTCCCCGAACTTATGTCCATCAAGGACTCTCTCATCAAGTATGTCTTCAAGCCAAATCAGAAAACTTCTGCAGCTTAA

Protein sequence

MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTSAA
Homology
BLAST of MC06g2133 vs. ExPASy Swiss-Prot
Match: Q9LQ04 (Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase OS=Arabidopsis thaliana OX=3702 GN=NRS/ER PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 5.0e-149
Identity = 250/297 (84.18%), Postives = 278/297 (93.60%), Query Frame = 0

Query: 3   ILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVK 62
           ++AD   +  +SF FLIYG+TGWIGGLLGKLCE QGI ++YGSGRL++R S+ ADI +VK
Sbjct: 1   MVADANGSSSSSFNFLIYGKTGWIGGLLGKLCEAQGITYTYGSGRLQDRQSIVADIESVK 60

Query: 63  PTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYD 122
           P+HVFNAAGVTGRPNVDWCESHKVETIRTNV GTL+LAD+CRE+GLVLINYATGCIFEYD
Sbjct: 61  PSHVFNAAGVTGRPNVDWCESHKVETIRTNVAGTLTLADICREKGLVLINYATGCIFEYD 120

Query: 123 SAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNF 182
           S HPL SGIGFKEE+TPNF GSFYSKTKAMVE+LLKNYENVCTLRVRMPISSDL+NPRNF
Sbjct: 121 SGHPLGSGIGFKEEDTPNFTGSFYSKTKAMVEELLKNYENVCTLRVRMPISSDLTNPRNF 180

Query: 183 ITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFI 242
           ITKI +YEKVVDIPNSMTILDELLPISIEMAKRNLTGI+NFTNPGVVSHNEILEMY+ +I
Sbjct: 181 ITKIARYEKVVDIPNSMTILDELLPISIEMAKRNLTGIYNFTNPGVVSHNEILEMYRDYI 240

Query: 243 DPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           DP+FTWKNF LEEQAKVIVAPRSNNELDA+KLK EFPELMSIK+SLIK+VF+PN+KT
Sbjct: 241 DPSFTWKNFTLEEQAKVIVAPRSNNELDATKLKTEFPELMSIKESLIKFVFEPNKKT 297

BLAST of MC06g2133 vs. ExPASy Swiss-Prot
Match: Q9LH76 (Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM3 OS=Arabidopsis thaliana OX=3702 GN=RHM3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 4.0e-138
Identity = 229/293 (78.16%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 7   GGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHV 66
           G S    S +FLIYG+TGW+GGLLGKLCE+QGI + YG GRLE+RASL ADI ++KP+HV
Sbjct: 372 GCSGDKRSLKFLIYGKTGWLGGLLGKLCEKQGIPYEYGKGRLEDRASLIADIRSIKPSHV 431

Query: 67  FNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHP 126
           FNAAG+TGRPNVDWCESHK ETIR NV GTL+LADVCRE  L+++N+ATGCIFEYD+AHP
Sbjct: 432 FNAAGLTGRPNVDWCESHKTETIRVNVAGTLTLADVCRENDLLMMNFATGCIFEYDAAHP 491

Query: 127 LNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITKI 186
             SGIGFKEE+ PNF GSFYSKTKAMVE+LL+ ++NVCTLRVRMPISSDL+NPRNFITKI
Sbjct: 492 EGSGIGFKEEDKPNFTGSFYSKTKAMVEELLREFDNVCTLRVRMPISSDLNNPRNFITKI 551

Query: 187 TKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNF 246
           ++Y KVV+IPNSMTILDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMYK +I+P+F
Sbjct: 552 SRYNKVVNIPNSMTILDELLPISIEMAKRNLRGIWNFTNPGVVSHNEILEMYKSYIEPDF 611

Query: 247 TWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
            W NFNLEEQAKVIVAPRSNNE+D +KL  EFPE++SIKDSLIKYVF+PN++T
Sbjct: 612 KWSNFNLEEQAKVIVAPRSNNEMDGAKLSKEFPEMLSIKDSLIKYVFEPNKRT 664

BLAST of MC06g2133 vs. ExPASy Swiss-Prot
Match: Q9SYM5 (Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1 OS=Arabidopsis thaliana OX=3702 GN=RHM1 PE=1 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 8.9e-138
Identity = 229/292 (78.42%), Postives = 261/292 (89.38%), Query Frame = 0

Query: 8   GSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHVF 67
           G+    S +FLIYG+TGWIGGLLGK+C++QGI + YG GRLE+R+SL  DI +VKPTHVF
Sbjct: 378 GTPQKPSLKFLIYGKTGWIGGLLGKICDKQGIAYEYGKGRLEDRSSLLQDIQSVKPTHVF 437

Query: 68  NAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHPL 127
           N+AGVTGRPNVDWCESHK ETIR NV GTL+LADVCRE GL+++N+ATGCIFEYD  HP 
Sbjct: 438 NSAGVTGRPNVDWCESHKTETIRANVAGTLTLADVCREHGLLMMNFATGCIFEYDDKHPE 497

Query: 128 NSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITKIT 187
            SGIGFKEE+TPNF GSFYSKTKAMVE+LLK Y+NVCTLRVRMPISSDL+NPRNFITKI+
Sbjct: 498 GSGIGFKEEDTPNFTGSFYSKTKAMVEELLKEYDNVCTLRVRMPISSDLNNPRNFITKIS 557

Query: 188 KYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNFT 247
           +Y KVV+IPNSMT+LDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMY+ +I+P F 
Sbjct: 558 RYNKVVNIPNSMTVLDELLPISIEMAKRNLKGIWNFTNPGVVSHNEILEMYRDYINPEFK 617

Query: 248 WKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           W NF LEEQAKVIVAPRSNNE+DASKLK EFPEL+SIK+SLIKY + PN+KT
Sbjct: 618 WANFTLEEQAKVIVAPRSNNEMDASKLKKEFPELLSIKESLIKYAYGPNKKT 669

BLAST of MC06g2133 vs. ExPASy Swiss-Prot
Match: Q9LPG6 (Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2 OS=Arabidopsis thaliana OX=3702 GN=RHM2 PE=1 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 4.4e-137
Identity = 226/294 (76.87%), Postives = 262/294 (89.12%), Query Frame = 0

Query: 6   DGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTH 65
           +G S   AS +FLIYG+TGW+GGLLGKLCE+QGI + YG GRLE+RASL ADI ++KPTH
Sbjct: 374 NGDSGDKASLKFLIYGKTGWLGGLLGKLCEKQGITYEYGKGRLEDRASLVADIRSIKPTH 433

Query: 66  VFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAH 125
           VFNAAG+TGRPNVDWCESHK ETIR NV GTL+LADVCRE  L+++N+ATGCIFEYD+ H
Sbjct: 434 VFNAAGLTGRPNVDWCESHKPETIRVNVAGTLTLADVCRENDLLMMNFATGCIFEYDATH 493

Query: 126 PLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITK 185
           P  SGIGFKEE+ PNF GSFYSKTKAMVE+LL+ ++NVCTLRVRMPISSDL+NPRNFITK
Sbjct: 494 PEGSGIGFKEEDKPNFFGSFYSKTKAMVEELLREFDNVCTLRVRMPISSDLNNPRNFITK 553

Query: 186 ITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPN 245
           I++Y KVVDIPNSMT+LDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMYK +I+P 
Sbjct: 554 ISRYNKVVDIPNSMTVLDELLPISIEMAKRNLRGIWNFTNPGVVSHNEILEMYKNYIEPG 613

Query: 246 FTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           F W NF +EEQAKVIVA RSNNE+D SKL  EFPE++SIK+SL+KYVF+PN++T
Sbjct: 614 FKWSNFTVEEQAKVIVAARSNNEMDGSKLSKEFPEMLSIKESLLKYVFEPNKRT 667

BLAST of MC06g2133 vs. ExPASy Swiss-Prot
Match: Q5UPS5 (Uncharacterized protein L780 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L780 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 114/285 (40.00%), Postives = 174/285 (61.05%), Query Frame = 0

Query: 16  RFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHVFNAAGVT-- 75
           ++LI+G  GWIG ++ K+ EQQG        R ++ +++E +I+ +KP  V +  G T  
Sbjct: 2   KWLIFGNKGWIGSMVSKILEQQGEQVVGAQSRADDESAVEREISEIKPDRVMSFIGRTHG 61

Query: 76  -GRPNVDWCE--SHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHPLNSG 135
            G   +D+ E     VE ++ N+ G L LA +C++  + L    TGCIFE  +    +  
Sbjct: 62  PGYSTIDYLEQSGKLVENVKDNLYGPLCLAFICQKYNIHLTYLGTGCIFEGQNNFSADEK 121

Query: 136 IGFKEEETPNFIGSFYSKTKAMVEDLLKNYEN-VCTLRVRMPISSDLSNPRNFITKITKY 195
            GF E + PNF GS YS  K   + L+  ++N V  LR+RMPI+ +  NPR+FITKI  Y
Sbjct: 122 -GFTENDKPNFFGSSYSVVKGFTDRLMHFFDNDVLNLRIRMPITIE-QNPRSFITKILSY 181

Query: 196 EKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNFTWK 255
            ++  IPNSMTILD+++P+ I+MA+   TG +NFTNPG+VSHNEIL + +    PN TW+
Sbjct: 182 SRICSIPNSMTILDQMIPVMIDMARNKTTGTFNFTNPGLVSHNEILSLIRDIHKPNLTWE 241

Query: 256 NFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFK 295
           N + E+Q  ++ A RSNN L+  KL++ +P++  I   + + V K
Sbjct: 242 NMSREQQLAILKADRSNNLLNTDKLQSLYPDVPDILTGIREVVSK 284

BLAST of MC06g2133 vs. NCBI nr
Match: XP_022159780.1 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase [Momordica charantia])

HSP 1 Score: 611 bits (1575), Expect = 1.36e-220
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA
Sbjct: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS
Sbjct: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 302

BLAST of MC06g2133 vs. NCBI nr
Match: XP_038875992.1 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase [Benincasa hispida])

HSP 1 Score: 579 bits (1492), Expect = 5.69e-208
Identity = 284/302 (94.04%), Postives = 295/302 (97.68%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILAD GS P  S +FLIYGRTGWIGGLLG LC+QQGIDF+YGSGRLENRASLEADIAA
Sbjct: 1   MGILADSGSGP--SLKFLIYGRTGWIGGLLGHLCQQQGIDFTYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTL+LADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLTLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKE++TPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEDDTPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNF L+EQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSLIKYVFKPNQKTS
Sbjct: 241 FIDPNFTWKNFTLDEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLIKYVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 300

BLAST of MC06g2133 vs. NCBI nr
Match: KAG6579471.1 (Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016940.1 Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 574 bits (1479), Expect = 5.45e-206
Identity = 283/302 (93.71%), Postives = 292/302 (96.69%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MG+LAD GSAP  S +FLIYGRTGWIGGLLGK+CEQQGI F+YGSGRLENR SLEADI A
Sbjct: 1   MGVLADSGSAP--SLKFLIYGRTGWIGGLLGKICEQQGIHFAYGSGRLENRVSLEADIDA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNF LEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSLIK VFKPNQKTS
Sbjct: 241 FIDPNFTWKNFTLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLIKNVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 300

BLAST of MC06g2133 vs. NCBI nr
Match: XP_008437195.1 (PREDICTED: bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase [Cucumis melo] >KAA0042806.1 bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase [Cucumis melo var. makuwa])

HSP 1 Score: 573 bits (1478), Expect = 7.75e-206
Identity = 281/302 (93.05%), Postives = 293/302 (97.02%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILAD GS   +S +FLIYGRTGWIGGLLG LC+QQGIDF+YGSGRLENRASLEADIAA
Sbjct: 1   MGILADSGSG--SSLKFLIYGRTGWIGGLLGHLCQQQGIDFTYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTL+LADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLTLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHP+NSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPINSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNFNLEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSL+KYVFKPNQKT 
Sbjct: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLLKYVFKPNQKTP 300

Query: 301 AA 302
            A
Sbjct: 301 TA 300

BLAST of MC06g2133 vs. NCBI nr
Match: XP_023549712.1 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572 bits (1475), Expect = 2.22e-205
Identity = 282/302 (93.38%), Postives = 292/302 (96.69%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MG+LAD GSAP  S +FLIYGRTGWIGGLLGK+CEQQGI F+YGSGRLENR SLEADI A
Sbjct: 1   MGVLADSGSAP--SLKFLIYGRTGWIGGLLGKICEQQGIHFAYGSGRLENRVSLEADIDA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNF LEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIK+SLIK VFKPNQKTS
Sbjct: 241 FIDPNFTWKNFTLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKESLIKNVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 300

BLAST of MC06g2133 vs. ExPASy TrEMBL
Match: A0A6J1DZR3 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase OS=Momordica charantia OX=3673 GN=LOC111026099 PE=4 SV=1)

HSP 1 Score: 611 bits (1575), Expect = 6.59e-221
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA
Sbjct: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS
Sbjct: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 302

BLAST of MC06g2133 vs. ExPASy TrEMBL
Match: A0A5A7TLE8 (Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003090 PE=4 SV=1)

HSP 1 Score: 573 bits (1478), Expect = 3.75e-206
Identity = 281/302 (93.05%), Postives = 293/302 (97.02%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILAD GS   +S +FLIYGRTGWIGGLLG LC+QQGIDF+YGSGRLENRASLEADIAA
Sbjct: 1   MGILADSGSG--SSLKFLIYGRTGWIGGLLGHLCQQQGIDFTYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTL+LADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLTLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHP+NSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPINSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNFNLEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSL+KYVFKPNQKT 
Sbjct: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLLKYVFKPNQKTP 300

Query: 301 AA 302
            A
Sbjct: 301 TA 300

BLAST of MC06g2133 vs. ExPASy TrEMBL
Match: A0A1S3AU07 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase OS=Cucumis melo OX=3656 GN=LOC103482697 PE=4 SV=1)

HSP 1 Score: 573 bits (1478), Expect = 3.75e-206
Identity = 281/302 (93.05%), Postives = 293/302 (97.02%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MGILAD GS   +S +FLIYGRTGWIGGLLG LC+QQGIDF+YGSGRLENRASLEADIAA
Sbjct: 1   MGILADSGSG--SSLKFLIYGRTGWIGGLLGHLCQQQGIDFTYGSGRLENRASLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTL+LADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLTLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHP+NSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPINSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNFNLEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSL+KYVFKPNQKT 
Sbjct: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLLKYVFKPNQKTP 300

Query: 301 AA 302
            A
Sbjct: 301 TA 300

BLAST of MC06g2133 vs. ExPASy TrEMBL
Match: A0A6J1E3P2 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111430302 PE=4 SV=1)

HSP 1 Score: 572 bits (1473), Expect = 2.17e-205
Identity = 282/302 (93.38%), Postives = 291/302 (96.36%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MG+LAD GSAP  S +FLIYGRTGWIGGLLGK+CEQQGI F+YGSGRLENR SLEADI A
Sbjct: 1   MGVLADSGSAP--SLKFLIYGRTGWIGGLLGKICEQQGIHFAYGSGRLENRVSLEADIDA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YD AHPLNSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDLAHPLNSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNF LEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIKDSLIK VFKPNQKTS
Sbjct: 241 FIDPNFTWKNFTLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKDSLIKNVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 300

BLAST of MC06g2133 vs. ExPASy TrEMBL
Match: A0A6J1HYZ2 (bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111468931 PE=4 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 3.08e-205
Identity = 282/302 (93.38%), Postives = 292/302 (96.69%), Query Frame = 0

Query: 1   MGILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAA 60
           MG+LAD  SAP  S +FLIYGRTGWIGGLLGK+CEQQGI F+YGSGRLENR SLEADIAA
Sbjct: 1   MGVLADSVSAP--SLKFLIYGRTGWIGGLLGKICEQQGIHFAYGSGRLENRVSLEADIAA 60

Query: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFE 120
           VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGL+LINYATGCIFE
Sbjct: 61  VKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLILINYATGCIFE 120

Query: 121 YDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180
           YDSAHPLNSGIGFKE+E PNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR
Sbjct: 121 YDSAHPLNSGIGFKEDEIPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPR 180

Query: 181 NFITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240
           NFITKIT+YEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ
Sbjct: 181 NFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQ 240

Query: 241 FIDPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKTS 300
           FIDPNFTWKNF LEEQAKVIVAPRSNNELDA+KLKNEFPEL+SIK+SLIK VFKPNQKTS
Sbjct: 241 FIDPNFTWKNFTLEEQAKVIVAPRSNNELDATKLKNEFPELLSIKESLIKNVFKPNQKTS 300

Query: 301 AA 302
           AA
Sbjct: 301 AA 300

BLAST of MC06g2133 vs. TAIR 10
Match: AT1G63000.1 (nucleotide-rhamnose synthase/epimerase-reductase )

HSP 1 Score: 528.5 bits (1360), Expect = 3.6e-150
Identity = 250/297 (84.18%), Postives = 278/297 (93.60%), Query Frame = 0

Query: 3   ILADGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVK 62
           ++AD   +  +SF FLIYG+TGWIGGLLGKLCE QGI ++YGSGRL++R S+ ADI +VK
Sbjct: 1   MVADANGSSSSSFNFLIYGKTGWIGGLLGKLCEAQGITYTYGSGRLQDRQSIVADIESVK 60

Query: 63  PTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYD 122
           P+HVFNAAGVTGRPNVDWCESHKVETIRTNV GTL+LAD+CRE+GLVLINYATGCIFEYD
Sbjct: 61  PSHVFNAAGVTGRPNVDWCESHKVETIRTNVAGTLTLADICREKGLVLINYATGCIFEYD 120

Query: 123 SAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNF 182
           S HPL SGIGFKEE+TPNF GSFYSKTKAMVE+LLKNYENVCTLRVRMPISSDL+NPRNF
Sbjct: 121 SGHPLGSGIGFKEEDTPNFTGSFYSKTKAMVEELLKNYENVCTLRVRMPISSDLTNPRNF 180

Query: 183 ITKITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFI 242
           ITKI +YEKVVDIPNSMTILDELLPISIEMAKRNLTGI+NFTNPGVVSHNEILEMY+ +I
Sbjct: 181 ITKIARYEKVVDIPNSMTILDELLPISIEMAKRNLTGIYNFTNPGVVSHNEILEMYRDYI 240

Query: 243 DPNFTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           DP+FTWKNF LEEQAKVIVAPRSNNELDA+KLK EFPELMSIK+SLIK+VF+PN+KT
Sbjct: 241 DPSFTWKNFTLEEQAKVIVAPRSNNELDATKLKTEFPELMSIKESLIKFVFEPNKKT 297

BLAST of MC06g2133 vs. TAIR 10
Match: AT3G14790.1 (rhamnose biosynthesis 3 )

HSP 1 Score: 492.3 bits (1266), Expect = 2.8e-139
Identity = 229/293 (78.16%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 7   GGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHV 66
           G S    S +FLIYG+TGW+GGLLGKLCE+QGI + YG GRLE+RASL ADI ++KP+HV
Sbjct: 372 GCSGDKRSLKFLIYGKTGWLGGLLGKLCEKQGIPYEYGKGRLEDRASLIADIRSIKPSHV 431

Query: 67  FNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHP 126
           FNAAG+TGRPNVDWCESHK ETIR NV GTL+LADVCRE  L+++N+ATGCIFEYD+AHP
Sbjct: 432 FNAAGLTGRPNVDWCESHKTETIRVNVAGTLTLADVCRENDLLMMNFATGCIFEYDAAHP 491

Query: 127 LNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITKI 186
             SGIGFKEE+ PNF GSFYSKTKAMVE+LL+ ++NVCTLRVRMPISSDL+NPRNFITKI
Sbjct: 492 EGSGIGFKEEDKPNFTGSFYSKTKAMVEELLREFDNVCTLRVRMPISSDLNNPRNFITKI 551

Query: 187 TKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNF 246
           ++Y KVV+IPNSMTILDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMYK +I+P+F
Sbjct: 552 SRYNKVVNIPNSMTILDELLPISIEMAKRNLRGIWNFTNPGVVSHNEILEMYKSYIEPDF 611

Query: 247 TWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
            W NFNLEEQAKVIVAPRSNNE+D +KL  EFPE++SIKDSLIKYVF+PN++T
Sbjct: 612 KWSNFNLEEQAKVIVAPRSNNEMDGAKLSKEFPEMLSIKDSLIKYVFEPNKRT 664

BLAST of MC06g2133 vs. TAIR 10
Match: AT1G78570.1 (rhamnose biosynthesis 1 )

HSP 1 Score: 491.1 bits (1263), Expect = 6.3e-139
Identity = 229/292 (78.42%), Postives = 261/292 (89.38%), Query Frame = 0

Query: 8   GSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTHVF 67
           G+    S +FLIYG+TGWIGGLLGK+C++QGI + YG GRLE+R+SL  DI +VKPTHVF
Sbjct: 378 GTPQKPSLKFLIYGKTGWIGGLLGKICDKQGIAYEYGKGRLEDRSSLLQDIQSVKPTHVF 437

Query: 68  NAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAHPL 127
           N+AGVTGRPNVDWCESHK ETIR NV GTL+LADVCRE GL+++N+ATGCIFEYD  HP 
Sbjct: 438 NSAGVTGRPNVDWCESHKTETIRANVAGTLTLADVCREHGLLMMNFATGCIFEYDDKHPE 497

Query: 128 NSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITKIT 187
            SGIGFKEE+TPNF GSFYSKTKAMVE+LLK Y+NVCTLRVRMPISSDL+NPRNFITKI+
Sbjct: 498 GSGIGFKEEDTPNFTGSFYSKTKAMVEELLKEYDNVCTLRVRMPISSDLNNPRNFITKIS 557

Query: 188 KYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPNFT 247
           +Y KVV+IPNSMT+LDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMY+ +I+P F 
Sbjct: 558 RYNKVVNIPNSMTVLDELLPISIEMAKRNLKGIWNFTNPGVVSHNEILEMYRDYINPEFK 617

Query: 248 WKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           W NF LEEQAKVIVAPRSNNE+DASKLK EFPEL+SIK+SLIKY + PN+KT
Sbjct: 618 WANFTLEEQAKVIVAPRSNNEMDASKLKKEFPELLSIKESLIKYAYGPNKKT 669

BLAST of MC06g2133 vs. TAIR 10
Match: AT1G53500.1 (NAD-dependent epimerase/dehydratase family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 3.1e-138
Identity = 226/294 (76.87%), Postives = 262/294 (89.12%), Query Frame = 0

Query: 6   DGGSAPDASFRFLIYGRTGWIGGLLGKLCEQQGIDFSYGSGRLENRASLEADIAAVKPTH 65
           +G S   AS +FLIYG+TGW+GGLLGKLCE+QGI + YG GRLE+RASL ADI ++KPTH
Sbjct: 374 NGDSGDKASLKFLIYGKTGWLGGLLGKLCEKQGITYEYGKGRLEDRASLVADIRSIKPTH 433

Query: 66  VFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIFEYDSAH 125
           VFNAAG+TGRPNVDWCESHK ETIR NV GTL+LADVCRE  L+++N+ATGCIFEYD+ H
Sbjct: 434 VFNAAGLTGRPNVDWCESHKPETIRVNVAGTLTLADVCRENDLLMMNFATGCIFEYDATH 493

Query: 126 PLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNPRNFITK 185
           P  SGIGFKEE+ PNF GSFYSKTKAMVE+LL+ ++NVCTLRVRMPISSDL+NPRNFITK
Sbjct: 494 PEGSGIGFKEEDKPNFFGSFYSKTKAMVEELLREFDNVCTLRVRMPISSDLNNPRNFITK 553

Query: 186 ITKYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYKQFIDPN 245
           I++Y KVVDIPNSMT+LDELLPISIEMAKRNL GIWNFTNPGVVSHNEILEMYK +I+P 
Sbjct: 554 ISRYNKVVDIPNSMTVLDELLPISIEMAKRNLRGIWNFTNPGVVSHNEILEMYKNYIEPG 613

Query: 246 FTWKNFNLEEQAKVIVAPRSNNELDASKLKNEFPELMSIKDSLIKYVFKPNQKT 300
           F W NF +EEQAKVIVA RSNNE+D SKL  EFPE++SIK+SL+KYVF+PN++T
Sbjct: 614 FKWSNFTVEEQAKVIVAARSNNEMDGSKLSKEFPEMLSIKESLLKYVFEPNKRT 667

BLAST of MC06g2133 vs. TAIR 10
Match: AT5G59290.1 (UDP-glucuronic acid decarboxylase 3 )

HSP 1 Score: 56.2 bits (134), Expect = 5.2e-08
Identity = 61/265 (23.02%), Postives = 112/265 (42.26%), Query Frame = 0

Query: 14  SFRFLIYGRTGWIGG-LLGKLCEQQG----IDFSYGSGRLENRAS---------LEADIA 73
           + R LI G  G+IG  L+ KL E +     +  +Y +G  EN            +  D+ 
Sbjct: 29  NMRILISGGAGFIGSHLVDKLMENEKNEVVVADNYFTGSKENLKKWIGHPRFELIRHDVT 88

Query: 74  AVKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLSLADVCRERGLVLINYATGCIF 133
                 V     +    +  + + + V+TI+TNV+GTL++  + +  G  ++  +T  ++
Sbjct: 89  EPLLIEVDRIYHLACPASPIFYKYNPVKTIKTNVIGTLNMLGLAKRVGARILLTSTSEVY 148

Query: 134 EYDSAHPLNSGIGFKEEETPNFIGSFYSKTKAMVEDLLKNYENVCTLRVRMPISSDLSNP 193
                HP      +     P  + S Y + K + E L+ +Y     + +R+    +   P
Sbjct: 149 GDPLIHPQPE--SYWGNVNPIGVRSCYDEGKRVAETLMFDYHRQHGIEIRIARIFNTYGP 208

Query: 194 R----------NFITKITKYEKV-VDIPNSMT----ILDELLPISIEMAKRNLTGIWNFT 250
           R          NFI +  + E + V  P + T     + +++   I + + N TG  N  
Sbjct: 209 RMNIDDGRVVSNFIAQALRGEALTVQKPGTQTRSFCYVSDMVDGLIRLMEGNDTGPINIG 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQ045.0e-14984.18Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
Q9LH764.0e-13878.16Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epime... [more]
Q9SYM58.9e-13878.42Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epime... [more]
Q9LPG64.4e-13776.87Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epime... [more]
Q5UPS52.9e-5640.00Uncharacterized protein L780 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MI... [more]
Match NameE-valueIdentityDescription
XP_022159780.11.36e-220100.00bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
XP_038875992.15.69e-20894.04bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
KAG6579471.15.45e-20693.71Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
XP_008437195.17.75e-20693.05PREDICTED: bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamn... [more]
XP_023549712.12.22e-20593.38bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
Match NameE-valueIdentityDescription
A0A6J1DZR36.59e-221100.00bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
A0A5A7TLE83.75e-20693.05Bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
A0A1S3AU073.75e-20693.05bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
A0A6J1E3P22.17e-20593.38bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
A0A6J1HYZ23.08e-20593.38bifunctional dTDP-4-dehydrorhamnose 3,5-epimerase/dTDP-4-dehydrorhamnose reducta... [more]
Match NameE-valueIdentityDescription
AT1G63000.13.6e-15084.18nucleotide-rhamnose synthase/epimerase-reductase [more]
AT3G14790.12.8e-13978.16rhamnose biosynthesis 3 [more]
AT1G78570.16.3e-13978.42rhamnose biosynthesis 1 [more]
AT1G53500.13.1e-13876.87NAD-dependent epimerase/dehydratase family protein [more]
AT5G59290.15.2e-0823.02UDP-glucuronic acid decarboxylase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 10..302
e-value: 8.3E-82
score: 276.7
NoneNo IPR availablePANTHERPTHR43000DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATEDcoord: 10..297
NoneNo IPR availablePANTHERPTHR43000:SF26BNAC05G13120D PROTEINcoord: 10..297
NoneNo IPR availableCDDcd05254dTDP_HR_like_SDR_ecoord: 16..288
e-value: 1.17688E-62
score: 197.849
IPR029903RmlD-like substrate binding domainPFAMPF04321RmlD_sub_bindcoord: 16..188
e-value: 1.4E-13
score: 50.6
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 15..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g2133.1MC06g2133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019305 dTDP-rhamnose biosynthetic process
biological_process GO:0010253 UDP-rhamnose biosynthetic process
molecular_function GO:0008830 dTDP-4-dehydrorhamnose 3,5-epimerase activity
molecular_function GO:0008831 dTDP-4-dehydrorhamnose reductase activity