MC06g0534 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0534
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Defective in cullin neddylation protein
Location	MC06: 4399723 .. 4401057 (-)
RNA-Seq Expression	MC06g0534
Synteny	MC06g0534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGCAAGAAGTCCATCCAACTTCGAAGATTTCTATTGCTACTCCTTCCAATTCAACCTCACCGGTAAGCTTCCACAAATGACTAACATTAGTATCAATTTTTTTTTTTTTTTTTTGCGACTGTTGTTGAAGTTTTGACAGGTAGAAGAGAGAAATGTAGAAATAGACATATATTTTTTAAAAGATAAGGATTTGTAAAATTCTTAATGGACAGAGTTGATGTGTTTTTTATTGTAGAGGAGAAGCAGAAAATCTTGGATGTAGCTACTGTATGCCAGCTTTTGGATCTTGTCATGGGACCTCGATTCCGTCCTCAAGTCCAATTACTAATCAACTTTCTCAAGGTACCAATGAATAATAGGACAATATTTGAGTGATAATACTAGGATAATCTCATATCTAATCAATTTCATTTTTCATATTACTCCAAACCTAACTAACTTCCCTTGAATTAAATGCAAGACCCAAATAGAGTATAAAGGGCTCAATATGGATCACTGGACTAGCATCTTCCGTTTCTTCAACCAGGTACTCATACAACACTATAGATACCATAGACATATGTTCACCTCCGAACTTTCAAGATTATGTTTAATAAATCTGAAGCTTAAAAACATCCTATTAAATCTCTAAACTTCAAGACTTTTTATCTAATAGGGTTCCTATCGCATTCAATTTTCTTCTCTTTTCTTATAAAATTTATGGAGTATTAGACACAGTGAACTATAACATTCCAAATTTATATTTAATAGGACCACGAACTTAAAATTTTAAATATGAGAAGAATCTATGAGCCACAAAATTAAAAGTTCAGAGACTTATCATATACTTTTAAAACTTCATGAATTTATTAGACACAAAATTGAACGTTCATTGACTAATAACACCTTTTAAAGTTCAAAAATCTATTTGGTAAAAGTAGAAGAAGTTTATGTACTAAACTTATAATTTAGTCTTAATTTTATCACTAATAAAACAACAATTTTGTAATGAATGTAACAATAAATTTGAGAATTATTTTTTTCACATTCAATTGAAAGTTTTTTGTATATATCAAGAGTAAAATTGATAATTTTAAAAGTTCAAAGATCGATTTTGAGAAGAGTATAGAGCATATGATGTATTCAACTAAAATTTTAAAATACGATAAATTAAATATTATCTAAAAGATTAGGACCTATTTAGAGTTGAATTGCAATTTTATTATTTTACTATTATTTATTTTAACCGATTGCGTGTTGCCGGCGGCAGGTCAGTTTTCCGGATCTAGAGAACTACGATCCACACCAAGGTTGGCCGGCGATTCTTGATAACTTTGTTGAATGGCTCAAAGCA

mRNA sequence

AAGGCAAGAAGTCCATCCAACTTCGAAGATTTCTATTGCTACTCCTTCCAATTCAACCTCACCGAGGAGAAGCAGAAAATCTTGGATGTAGCTACTGTATGCCAGCTTTTGGATCTTGTCATGGGACCTCGATTCCGTCCTCAAGTCCAATTACTAATCAACTTTCTCAAGACCCAAATAGAGTATAAAGGGCTCAATATGGATCACTGGACTAGCATCTTCCGTTTCTTCAACCAGGTCAGTTTTCCGGATCTAGAGAACTACGATCCACACCAAGGTTGGCCGGCGATTCTTGATAACTTTGTTGAATGGCTCAAAGCA

Coding sequence (CDS)

AAGGCAAGAAGTCCATCCAACTTCGAAGATTTCTATTGCTACTCCTTCCAATTCAACCTCACCGAGGAGAAGCAGAAAATCTTGGATGTAGCTACTGTATGCCAGCTTTTGGATCTTGTCATGGGACCTCGATTCCGTCCTCAAGTCCAATTACTAATCAACTTTCTCAAGACCCAAATAGAGTATAAAGGGCTCAATATGGATCACTGGACTAGCATCTTCCGTTTCTTCAACCAGGTCAGTTTTCCGGATCTAGAGAACTACGATCCACACCAAGGTTGGCCGGCGATTCTTGATAACTTTGTTGAATGGCTCAAAGCA

Protein sequence

KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLKA

Homology

[Close]

BLAST of MC06g0534 vs. ExPASy Swiss-Prot
Match: Q8CCA0 (DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-11
Identity = 39/101 (38.61%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 6   SNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGL 65
           +NF+  Y Y+F F   E+ Q+ LD+ T   +L L++G +  P   +   FL+ Q +YK +
Sbjct: 191 TNFKLIYRYAFDF-AREKDQRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVI 250

Query: 66  NMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           N D W ++  F   +S  DL NYD    WP +LD FVEW K
Sbjct: 251 NKDQWCNVLEFSRTISL-DLSNYDEDGAWPVLLDEFVEWYK 287

[Close]

BLAST of MC06g0534 vs. ExPASy Swiss-Prot
Match: Q92564 (DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 3.0e-11
Identity = 38/101 (37.62%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 6   SNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGL 65
           +NF+  Y Y+F F   E+ Q+ LD+ T   +L L++G +  P   +   FL+ Q +YK +
Sbjct: 191 TNFKLIYRYAFDF-AREKDQRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVI 250

Query: 66  NMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           N D W ++  F   ++  DL NYD    WP +LD FVEW K
Sbjct: 251 NKDQWCNVLEFSRTINL-DLSNYDEDGAWPVLLDEFVEWYK 287

[Close]

BLAST of MC06g0534 vs. ExPASy Swiss-Prot
Match: Q9BTE7 (DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 8.6e-11
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 6   SNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGL 65
           S+F++ Y Y+F F   ++ Q+ LD+ T   +L L++G R  P   +   +L+ Q +Y+ +
Sbjct: 136 SSFKNIYRYAFDF-ARDKDQRSLDIDTAKSMLALLLG-RTWPLFSVFYQYLE-QSKYRVM 195

Query: 66  NMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           N D W ++  F   V   DL NYD    WP +LD FVEW K
Sbjct: 196 NKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVEWQK 232

[Close]

BLAST of MC06g0534 vs. ExPASy Swiss-Prot
Match: Q9CXV9 (DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 8.6e-11
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 6   SNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGL 65
           S+F++ Y Y+F F   ++ Q+ LD+ T   +L L++G R  P   +   +L+ Q +Y+ +
Sbjct: 136 SSFKNIYRYAFDF-ARDKDQRSLDIDTAKSMLALLLG-RTWPLFSVFYQYLE-QSKYRVM 195

Query: 66  NMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           N D W ++  F   V   DL NYD    WP +LD FVEW K
Sbjct: 196 NKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVEWQK 232

[Close]

BLAST of MC06g0534 vs. ExPASy Swiss-Prot
Match: Q5PPL2 (DCN1-like protein 5 OS=Rattus norvegicus OX=10116 GN=Dcun1d5 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 8.6e-11
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 6   SNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGL 65
           S+F++ Y Y+F F   ++ Q+ LD+ T   +L L++G R  P   +   +L+ Q +Y+ +
Sbjct: 136 SSFKNIYRYAFDF-ARDKDQRSLDIDTAKSMLALLLG-RTWPLFSVFYQYLE-QSKYRVM 195

Query: 66  NMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           N D W ++  F   V   DL NYD    WP +LD FVEW K
Sbjct: 196 NKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVEWQK 232

[Close]

BLAST of MC06g0534 vs. NCBI nr
Match: GAY35620.1 (hypothetical protein CUMW_283240 [Citrus unshiu] >GAY35622.1 hypothetical protein CUMW_283240 [Citrus unshiu])

HSP 1 Score: 152 bits (383), Expect = 6.69e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 103 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 162

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 163 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 208

[Close]

BLAST of MC06g0534 vs. NCBI nr
Match: XP_006453676.1 (DCN1-like protein 4 isoform X2 [Citrus clementina] >XP_006473967.1 DCN1-like protein 4 isoform X2 [Citrus sinensis] >ESR66916.1 hypothetical protein CICLE_v10009421mg [Citrus clementina] >ESR66917.1 hypothetical protein CICLE_v10009421mg [Citrus clementina] >KDO59389.1 hypothetical protein CISIN_1g027440mg [Citrus sinensis] >KDO59390.1 hypothetical protein CISIN_1g027440mg [Citrus sinensis])

HSP 1 Score: 152 bits (383), Expect = 8.92e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 113 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 172

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 173 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 218

[Close]

BLAST of MC06g0534 vs. NCBI nr
Match: KDO59388.1 (hypothetical protein CISIN_1g027440mg [Citrus sinensis])

HSP 1 Score: 152 bits (383), Expect = 9.18e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 114 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 173

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 174 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 219

[Close]

BLAST of MC06g0534 vs. NCBI nr
Match: XP_006473966.1 (DCN1-like protein 4 isoform X1 [Citrus sinensis] >XP_024033439.1 DCN1-like protein 4 isoform X1 [Citrus clementina] >KDO59387.1 hypothetical protein CISIN_1g027440mg [Citrus sinensis])

HSP 1 Score: 152 bits (383), Expect = 9.18e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 114 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 173

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 174 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 219

[Close]

BLAST of MC06g0534 vs. NCBI nr
Match: KAA8540533.1 (hypothetical protein F0562_024548 [Nyssa sinensis])

HSP 1 Score: 151 bits (381), Expect = 2.25e-43
Identity = 63/107 (58.88%), Postives = 82/107 (76.64%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + + PSNF DFY Y+FQ+ LTEEKQK +D+ ++C+LLDLV+G +FR QV   + +LKTQI
Sbjct: 121 EVKRPSNFVDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFRAQVDSFVEYLKTQI 180

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLKA 107
           +YK +NMD W   FRF N++SFPD  NYDP   WP ILDNFVEW++A
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDFSNYDPELAWPLILDNFVEWMRA 227

[Close]

BLAST of MC06g0534 vs. ExPASy TrEMBL
Match: A0A2H5N5X9 (Defective in cullin neddylation protein OS=Citrus unshiu OX=55188 GN=CUMW_283240 PE=4 SV=1)

HSP 1 Score: 152 bits (383), Expect = 3.24e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 103 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 162

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 163 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 208

[Close]

BLAST of MC06g0534 vs. ExPASy TrEMBL
Match: V4UW29 (Defective in cullin neddylation protein OS=Citrus clementina OX=85681 GN=CICLE_v10009421mg PE=4 SV=1)

HSP 1 Score: 152 bits (383), Expect = 4.32e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 113 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 172

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 173 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 218

[Close]

BLAST of MC06g0534 vs. ExPASy TrEMBL
Match: A0A067EZU1 (Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g027440mg PE=4 SV=1)

HSP 1 Score: 152 bits (383), Expect = 4.32e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 113 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 172

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 173 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 218

[Close]

BLAST of MC06g0534 vs. ExPASy TrEMBL
Match: A0A067EWB4 (Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g027440mg PE=4 SV=1)

HSP 1 Score: 152 bits (383), Expect = 4.44e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 114 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 173

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 174 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 219

[Close]

BLAST of MC06g0534 vs. ExPASy TrEMBL
Match: A0A067F7B5 (Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g027440mg PE=4 SV=1)

HSP 1 Score: 152 bits (383), Expect = 4.44e-44
Identity = 64/106 (60.38%), Postives = 86/106 (81.13%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R+P NF DFY ++F+++LTEEKQK +D+ T+C+LL+LV+GP+FR QV LLI++LK Q 
Sbjct: 114 EVRTPPNFADFYSFAFRYHLTEEKQKNIDIETICELLNLVLGPQFRRQVDLLIDYLKVQS 173

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 106
            YK +N+D W  IFRF N++SFPDLENYD  Q WP ILDNFV+WL+
Sbjct: 174 NYKVINLDQWLGIFRFCNEISFPDLENYDETQAWPLILDNFVDWLR 219

[Close]

BLAST of MC06g0534 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 141.0 bits (354), Expect = 5.7e-34
Identity = 58/106 (54.72%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R PSNF DFY Y+F + LTEEKQK +D+ T+CQLL++VMG  FR QV   + +LK Q 
Sbjct: 118 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 177

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           +YK +NMD W  ++RF N++SFPD+ +Y+P   WP ILDNFVEW++
Sbjct: 178 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQ 223

[Close]

BLAST of MC06g0534 vs. TAIR 10
Match: AT1G15860.2 (Domain of unknown function (DUF298) )

HSP 1 Score: 141.0 bits (354), Expect = 5.7e-34
Identity = 58/106 (54.72%), Postives = 79/106 (74.53%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQI 60
           + R PSNF DFY Y+F + LTEEKQK +D+ T+CQLL++VMG  FR QV   + +LK Q 
Sbjct: 118 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 177

Query: 61  EYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
           +YK +NMD W  ++RF N++SFPD+ +Y+P   WP ILDNFVEW++
Sbjct: 178 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQ 223

[Close]

BLAST of MC06g0534 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 133.7 bits (335), Expect = 9.1e-32
Identity = 58/115 (50.43%), Postives = 79/115 (68.70%), Query Frame = 0


Query: 1   KARSPSNFEDFYCYSFQFNLTEEKQKILDVATVCQLLDLVMGPRFRPQVQLLINFLKT-- 60
           + R PSNF DFY Y+F + LTEEKQK +D+ T+CQLL++VMG  FR QV   + +LK   
Sbjct: 118 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWI 177

Query: 61  -------QIEYKGLNMDHWTSIFRFFNQVSFPDLENYDPHQGWPAILDNFVEWLK 107
                  Q +YK +NMD W  ++RF N++SFPD+ +Y+P   WP ILDNFVEW++
Sbjct: 178 TQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQ 232

[Close]

BLAST of MC06g0534 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-07
Identity = 32/93 (34.41%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 14  YSFQFNLTEEK-QKILDVATVCQLLDLVMGPRFRPQVQLLINFLKTQIEYKGLNMDHWTS 73
           Y+F F   +EK QK L + T   +  L+   R  P V    +FL+ +   K ++ D W  
Sbjct: 152 YNFAFGWAKEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDR-HNKAISKDTWAQ 211

Query: 74  IFRFFNQVSFPDLENYDPHQGWPAILDNFVEWL 106
           +  F   V  P L NYD    WP ++D FVE+L
Sbjct: 212 LLEFSRMVD-PVLSNYDAEGAWPYLIDEFVEYL 242

The following BLAST results are available for this feature:

BLAST of MC06g0534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8CCA0	1.3e-11	38.61	DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1	[more]
Q92564	3.0e-11	37.62	DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2	[more]
Q9BTE7	8.6e-11	36.63	DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1	[more]
Q9CXV9	8.6e-11	36.63	DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1	[more]
Q5PPL2	8.6e-11	36.63	DCN1-like protein 5 OS=Rattus norvegicus OX=10116 GN=Dcun1d5 PE=2 SV=1	[more]

BLAST of MC06g0534 vs. NCBI nr


Match Name	E-value	Identity	Description
GAY35620.1	6.69e-44	60.38	hypothetical protein CUMW_283240 [Citrus unshiu] >GAY35622.1 hypothetical protei...	[more]
XP_006453676.1	8.92e-44	60.38	DCN1-like protein 4 isoform X2 [Citrus clementina] >XP_006473967.1 DCN1-like pro...	[more]
KDO59388.1	9.18e-44	60.38	hypothetical protein CISIN_1g027440mg [Citrus sinensis]	[more]
XP_006473966.1	9.18e-44	60.38	DCN1-like protein 4 isoform X1 [Citrus sinensis] >XP_024033439.1 DCN1-like prote...	[more]
KAA8540533.1	2.25e-43	58.88	hypothetical protein F0562_024548 [Nyssa sinensis]	[more]

BLAST of MC06g0534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2H5N5X9	3.24e-44	60.38	Defective in cullin neddylation protein OS=Citrus unshiu OX=55188 GN=CUMW_283240...	[more]
V4UW29	4.32e-44	60.38	Defective in cullin neddylation protein OS=Citrus clementina OX=85681 GN=CICLE_v...	[more]
A0A067EZU1	4.32e-44	60.38	Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g02...	[more]
A0A067EWB4	4.44e-44	60.38	Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g02...	[more]
A0A067F7B5	4.44e-44	60.38	Defective in cullin neddylation protein OS=Citrus sinensis OX=2711 GN=CISIN_1g02...	[more]

BLAST of MC06g0534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15860.1	5.7e-34	54.72	Domain of unknown function (DUF298)	[more]
AT1G15860.2	5.7e-34	54.72	Domain of unknown function (DUF298)	[more]
AT1G15860.3	9.1e-32	50.43	Domain of unknown function (DUF298)	[more]
AT3G12760.1	1.2e-07	34.41	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 4..104 e-value: 1.0E-21 score: 77.6
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 1..106 score: 26.141155
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 5..107 e-value: 1.9E-24 score: 87.9
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 3..106
None	No IPR available	PANTHER	PTHR12281:SF12	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 3..106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0534.1	MC06g0534.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding