MC05g_new0388 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g_new0388 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.3e-60
Identity = 112/223 (50.22%), Postives = 160/223 (71.75%), Query Frame = 0

Query: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105
           MVGPVRP+ V+FGSSIVQ+SF D+GWGA +A+LY+R AD+ILRGYAGW+S  ALKV+ +V
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 106 FPKDSTIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIF 165
           FPKD+ IQP+LVIVYFGGND++   P+     VPL  F +NM KI +H+  LS  TR IF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 166 LTPPPINSEQLKQLMPGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWL- 225
           LTPPP+N +Q++ +     K R+ E    YA+ L+++C+++ V+ +D++ AIQ ++DWL 
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 226 TFFRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEF 264
           + F DG+HF  K S     EI  ++   +W P+L  +++P+EF
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF 223

BLAST of MC05g_new0388 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.8e-57
Identity = 111/227 (48.90%), Postives = 156/227 (68.72%), Query Frame = 0

Query: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105
           MVGP RP+ V+FGSSIVQ SF   GWGA ++ +YAR+AD+ILRGY GW+S +AL+VVD+V
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 106 FPKDSTIQPALVIVYFGGNDA----SINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIF 165
           FPKD+ +QP+LVIVYFGGND+    S      VPL  + DNM KI  H++ LS  TR IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 166 LTPPPINSEQLKQ-LMPGL-EKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDW 225
           L+ PP++  +++Q   P L E +RT +  + Y+DA +++C++LG+E VDLF   Q  +DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 226 LTF-FRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEFED 266
            T  F DG+H + +GS+    EI  ++ +  W P+L  ++MP EF D
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFAD 227

BLAST of MC05g_new0388 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 144.1 bits (362), Expect = 2.3e-33
Identity = 76/226 (33.63%), Postives = 125/226 (55.31%), Query Frame = 0

Query: 50  VRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKD 109
           +RP+ V+FG SI   SF   GWG+ +A+ Y+R+ADV++RGY G+++  AL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 110 STIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPP 169
           S+  P    ++FG NDA++    S    VP++ +TDN+ KIV H+K  S T   + +TPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 170 PINSEQLKQLMPGL-------EKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKED 229
           PI+    +     +       E  RT E   +YA   + + ++LG+  V+L+  +Q   D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 230 W-LTFFRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEF 264
           W   +  DG+H   +G+   FDE+  +  ++ W   L    MP +F
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVF-REAW---LSPEEMPFDF 222

BLAST of MC05g_new0388 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 78/212 (36.79%), Postives = 119/212 (56.13%), Query Frame = 0

Query: 50  VRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFP-- 109
           +R K  +FG SI + SF D GWGA +A+L  R+AD++LRGY+G+++  ALKVV++VFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 110 -KDSTIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIFL 169
            +D    PA V V+FG NDA +    S    VPL  +  N+  IV  +K     T  I +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 170 TPPPINSEQLKQLMPGLEKL-----RTMENLELYADALIDICKKLGVEYVDLFRAIQNKE 229
           TPPPI+ E+ +   P +E       RT E   LYA A I + ++  +   DL+  +Q   
Sbjct: 122 TPPPID-EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

Query: 230 DWLT-FFRDGVHFNEKGSRAAFDEIKNMINKK 249
           +W T    DG+H +  G++  F+E+   + ++
Sbjct: 182 NWQTECLWDGLHLSRVGNKVVFEEVAKKLKEE 212

BLAST of MC05g_new0388 vs. ExPASy Swiss-Prot
Match: Q2TAA2 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.8e-25
Identity = 58/204 (28.43%), Postives = 116/204 (56.86%), Query Frame = 0

Query: 52  PKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDST 111
           P+ ++FG SI QFSF   GWGA +A+   R+ DV+ RG++G+++  A  ++ ++  K ++
Sbjct: 16  PRLLLFGDSITQFSFQQGGWGASLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRKGNS 75

Query: 112 IQ-PALVIVYFGGNDASINFPN---SVPLDRFTDNMTKIVDHIKCLS-TTTRSIFLTPPP 171
           +  P  V ++FG ND+++   N    +PL+ +  N+  +V ++K +     R I +TP P
Sbjct: 76  LDIPVAVTIFFGANDSALKDENPKQHIPLEEYAANLKSMVQYLKSVDIPENRVILITPTP 135

Query: 172 INSEQLKQ--LMPGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFR 231
           +     ++  ++ G +  R    +  YA+A + + +  G + +DL+  +Q+ +D+ ++  
Sbjct: 136 LCETAWEEQCIIQGCKLNRLNSVVGEYANACLQVAQDCGTDVLDLWTLMQDSQDFSSYLS 195

Query: 232 DGVHFNEKGSRAAFDEIKNMINKK 249
           DG+H + KG+   F  +  +I KK
Sbjct: 196 DGLHLSPKGNEFLFSHLWPLIEKK 219

BLAST of MC05g_new0388 vs. NCBI nr
Match: XP_022145517.1 (GDSL esterase/lipase At2g38180-like [Momordica charantia])

HSP 1 Score: 547 bits (1410), Expect = 1.19e-196
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS
Sbjct: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE
Sbjct: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE
Sbjct: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IKNMINKKNWTPNLDARAMPIEFEDI
Sbjct: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266

BLAST of MC05g_new0388 vs. NCBI nr
Match: XP_022145516.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 472 bits (1215), Expect = 6.32e-167
Identity = 220/266 (82.71%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL+VGSGRCQLPKQHPS RPSLSI FP ALSR GWRTKMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLQVGSGRCQLPKQHPSLRPSLSIQFPGALSRRGWRTKMVGPVRPKFVLFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSFDDKGWGADIANLYARQADVILRGYAGWSS QA++VVDK+FPKDST QPALVIVY
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSSQAIQVVDKIFPKDSTTQPALVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDA+INFPNSVP+D + DNMTKI +HI CLSTTTRSIFLTPPP+NSEQLKQ MPGLE
Sbjct: 121 FGGNDAAINFPNSVPVDEYIDNMTKIAEHITCLSTTTRSIFLTPPPVNSEQLKQQMPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           K+RTMENLE+Y+DALID+C+K+GVEY+DLFR IQ  +DWLTFFRDGVHFNEKGSR  FDE
Sbjct: 181 KVRTMENLEIYSDALIDVCRKIGVEYIDLFRVIQRNDDWLTFFRDGVHFNEKGSRVVFDE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IK  IN+ NW P LDA++M  E+E I
Sbjct: 241 IKTSINRSNWNPPLDAKSMSTEYEHI 266

BLAST of MC05g_new0388 vs. NCBI nr
Match: XP_022145497.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 444 bits (1141), Expect = 2.12e-155
Identity = 211/266 (79.32%), Postives = 234/266 (87.97%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL VGSGRCQLPKQHPS RPSLS  FP ALSR GWRTKMVGP+RPKFV+FGSS
Sbjct: 1   MSCSLLNPLPVGSGRCQLPKQHPSLRPSLSTQFPGALSRCGWRTKMVGPMRPKFVVFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSF DKGWGA+IANLYARQADVILRGY+GW+S QAL+VVD+VFPKDS IQP+LVIVY
Sbjct: 61  IVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEVFPKDSIIQPSLVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDA INFP SVPL  FT NMTKI  HIK LSTTTR IFLTPPP+NSEQ+++L PGLE
Sbjct: 121 FGGNDAVINFPTSVPLSEFTVNMTKIASHIKNLSTTTRGIFLTPPPVNSEQIRELFPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           KLRTMENLE Y DALIDICK++GVEYVDLFRA+Q ++DWLTFF DGVHFNEKGS   F+E
Sbjct: 181 KLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEKGSHVVFEE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IK +IN+ NW P+L A +MPIEF +I
Sbjct: 241 IKTLINRANWVPSLAAGSMPIEFGNI 266

BLAST of MC05g_new0388 vs. NCBI nr
Match: XP_022145518.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 437 bits (1123), Expect = 1.35e-152
Identity = 208/267 (77.90%), Postives = 234/267 (87.64%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL+VGS RCQLPKQHPS RPSLSIHFP ALSR GWR KMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+S QAL+VVD+VFPKDS IQP+L IVY
Sbjct: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKDSIIQPSLAIVY 120

Query: 121 FGGNDASINFP----NSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLM 180
           FGGNDA I  P    + VPL +F DNMTKI  HIK LSTTTRSIFLTPPP+NS+Q+++L+
Sbjct: 121 FGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIFLTPPPVNSDQIRELL 180

Query: 181 PGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRA 240
           PGLEKLRTMENLE+Y DALIDIC+++GVEYVDLFRA+Q +EDWLTFF DGVHF E+GSR 
Sbjct: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240

Query: 241 AFDEIKNMINKKNWTPNLDARAMPIEF 263
            FDEIKN IN+ NW P+L   +MPIEF
Sbjct: 241 VFDEIKNTINRANWVPSLAVGSMPIEF 267

BLAST of MC05g_new0388 vs. NCBI nr
Match: XP_022145498.1 (GDSL esterase/lipase CPRD49-like [Momordica charantia])

HSP 1 Score: 430 bits (1106), Expect = 1.23e-149
Identity = 204/270 (75.56%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNP+ VGSGRCQLPKQH S RPSLS HFP ALSRHGWR KMVGPVRPKFV+FGSS
Sbjct: 25  MSCSLLNPVPVGSGRCQLPKQHLSLRPSLSTHFPGALSRHGWRAKMVGPVRPKFVLFGSS 84

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSF D+GWGADIA+LY+RQADVILRGY GW+S +AL+VVD++FPKDS IQP+LVIVY
Sbjct: 85  IVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEIFPKDSIIQPSLVIVY 144

Query: 121 FGGNDASINFP----NSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLM 180
           FGGNDA I  P    + VPL  F +NMT IV HIK LS TTRSIFLTPPP+NSEQ+++LM
Sbjct: 145 FGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIFLTPPPVNSEQIRELM 204

Query: 181 PGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRA 240
           PGLEKLRTMENLE+Y DA+IDIC+ +GVEYVDLFRA+Q ++DWLTFF DGVHFNE+GSR 
Sbjct: 205 PGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEEGSRV 264

Query: 241 AFDEIKNMINKKNWTPNLDARAMPIEFEDI 266
            F+EIKN IN+ NW P+L A +MPIEFE+I
Sbjct: 265 VFEEIKNTINRANWVPSLAAGSMPIEFENI 294

BLAST of MC05g_new0388 vs. ExPASy TrEMBL
Match: A0A6J1CWU2 (GDSL esterase/lipase At2g38180-like OS=Momordica charantia OX=3673 GN=LOC111014946 PE=4 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 5.76e-197
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS
Sbjct: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE
Sbjct: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE
Sbjct: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IKNMINKKNWTPNLDARAMPIEFEDI
Sbjct: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266

BLAST of MC05g_new0388 vs. ExPASy TrEMBL
Match: A0A6J1CWJ2 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014945 PE=4 SV=1)

HSP 1 Score: 472 bits (1215), Expect = 3.06e-167
Identity = 220/266 (82.71%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL+VGSGRCQLPKQHPS RPSLSI FP ALSR GWRTKMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLQVGSGRCQLPKQHPSLRPSLSIQFPGALSRRGWRTKMVGPVRPKFVLFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSFDDKGWGADIANLYARQADVILRGYAGWSS QA++VVDK+FPKDST QPALVIVY
Sbjct: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSSQAIQVVDKIFPKDSTTQPALVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDA+INFPNSVP+D + DNMTKI +HI CLSTTTRSIFLTPPP+NSEQLKQ MPGLE
Sbjct: 121 FGGNDAAINFPNSVPVDEYIDNMTKIAEHITCLSTTTRSIFLTPPPVNSEQLKQQMPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           K+RTMENLE+Y+DALID+C+K+GVEY+DLFR IQ  +DWLTFFRDGVHFNEKGSR  FDE
Sbjct: 181 KVRTMENLEIYSDALIDVCRKIGVEYIDLFRVIQRNDDWLTFFRDGVHFNEKGSRVVFDE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IK  IN+ NW P LDA++M  E+E I
Sbjct: 241 IKTSINRSNWNPPLDAKSMSTEYEHI 266

BLAST of MC05g_new0388 vs. ExPASy TrEMBL
Match: A0A6J1CWS0 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014934 PE=4 SV=1)

HSP 1 Score: 444 bits (1141), Expect = 1.03e-155
Identity = 211/266 (79.32%), Postives = 234/266 (87.97%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL VGSGRCQLPKQHPS RPSLS  FP ALSR GWRTKMVGP+RPKFV+FGSS
Sbjct: 1   MSCSLLNPLPVGSGRCQLPKQHPSLRPSLSTQFPGALSRCGWRTKMVGPMRPKFVVFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSF DKGWGA+IANLYARQADVILRGY+GW+S QAL+VVD+VFPKDS IQP+LVIVY
Sbjct: 61  IVQFSFADKGWGAEIANLYARQADVILRGYSGWNSSQALEVVDEVFPKDSIIQPSLVIVY 120

Query: 121 FGGNDASINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLMPGLE 180
           FGGNDA INFP SVPL  FT NMTKI  HIK LSTTTR IFLTPPP+NSEQ+++L PGLE
Sbjct: 121 FGGNDAVINFPTSVPLSEFTVNMTKIASHIKNLSTTTRGIFLTPPPVNSEQIRELFPGLE 180

Query: 181 KLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRAAFDE 240
           KLRTMENLE Y DALIDICK++GVEYVDLFRA+Q ++DWLTFF DGVHFNEKGS   F+E
Sbjct: 181 KLRTMENLEKYTDALIDICKEIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEKGSHVVFEE 240

Query: 241 IKNMINKKNWTPNLDARAMPIEFEDI 266
           IK +IN+ NW P+L A +MPIEF +I
Sbjct: 241 IKTLINRANWVPSLAAGSMPIEFGNI 266

BLAST of MC05g_new0388 vs. ExPASy TrEMBL
Match: A0A6J1CW58 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014947 PE=4 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 6.53e-153
Identity = 208/267 (77.90%), Postives = 234/267 (87.64%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNPL+VGS RCQLPKQHPS RPSLSIHFP ALSR GWR KMVGPVRPKFV+FGSS
Sbjct: 1   MSCSLLNPLQVGSSRCQLPKQHPSLRPSLSIHFPGALSRCGWRAKMVGPVRPKFVLFGSS 60

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQ+SFDD+GWGADIANLYARQADVILRGYAGW+S QAL+VVD+VFPKDS IQP+L IVY
Sbjct: 61  IVQYSFDDRGWGADIANLYARQADVILRGYAGWNSSQALQVVDEVFPKDSIIQPSLAIVY 120

Query: 121 FGGNDASINFP----NSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLM 180
           FGGNDA I  P    + VPL +F DNMTKI  HIK LSTTTRSIFLTPPP+NS+Q+++L+
Sbjct: 121 FGGNDAIIPLPFPGGSHVPLPQFIDNMTKIACHIKSLSTTTRSIFLTPPPVNSDQIRELL 180

Query: 181 PGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRA 240
           PGLEKLRTMENLE+Y DALIDIC+++GVEYVDLFRA+Q +EDWLTFF DGVHF E+GSR 
Sbjct: 181 PGLEKLRTMENLEIYTDALIDICREIGVEYVDLFRAVQKREDWLTFFTDGVHFTEEGSRV 240

Query: 241 AFDEIKNMINKKNWTPNLDARAMPIEF 263
            FDEIKN IN+ NW P+L   +MPIEF
Sbjct: 241 VFDEIKNTINRANWVPSLAVGSMPIEF 267

BLAST of MC05g_new0388 vs. ExPASy TrEMBL
Match: A0A6J1CW36 (GDSL esterase/lipase CPRD49-like OS=Momordica charantia OX=3673 GN=LOC111014935 PE=4 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 5.95e-150
Identity = 204/270 (75.56%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MSCSLLNPLRVGSGRCQLPKQHPSQRPSLSIHFPSALSRHGWRTKMVGPVRPKFVIFGSS 60
           MSCSLLNP+ VGSGRCQLPKQH S RPSLS HFP ALSRHGWR KMVGPVRPKFV+FGSS
Sbjct: 25  MSCSLLNPVPVGSGRCQLPKQHLSLRPSLSTHFPGALSRHGWRAKMVGPVRPKFVLFGSS 84

Query: 61  IVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKDSTIQPALVIVY 120
           IVQFSF D+GWGADIA+LY+RQADVILRGY GW+S +AL+VVD++FPKDS IQP+LVIVY
Sbjct: 85  IVQFSFGDRGWGADIADLYSRQADVILRGYGGWNSSRALQVVDEIFPKDSIIQPSLVIVY 144

Query: 121 FGGNDASINFP----NSVPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPPPINSEQLKQLM 180
           FGGNDA I  P    + VPL  F +NMT IV HIK LS TTRSIFLTPPP+NSEQ+++LM
Sbjct: 145 FGGNDAVIPLPFPGGSHVPLPEFINNMTNIVCHIKSLSATTRSIFLTPPPVNSEQIRELM 204

Query: 181 PGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWLTFFRDGVHFNEKGSRA 240
           PGLEKLRTMENLE+Y DA+IDIC+ +GVEYVDLFRA+Q ++DWLTFF DGVHFNE+GSR 
Sbjct: 205 PGLEKLRTMENLEIYTDAIIDICRDIGVEYVDLFRAVQRRDDWLTFFTDGVHFNEEGSRV 264

Query: 241 AFDEIKNMINKKNWTPNLDARAMPIEFEDI 266
            F+EIKN IN+ NW P+L A +MPIEFE+I
Sbjct: 265 VFEEIKNTINRANWVPSLAAGSMPIEFENI 294

BLAST of MC05g_new0388 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.6e-61
Identity = 112/223 (50.22%), Postives = 160/223 (71.75%), Query Frame = 0

Query: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105
           MVGPVRP+ V+FGSSIVQ+SF D+GWGA +A+LY+R AD+ILRGYAGW+S  ALKV+ +V
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 106 FPKDSTIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIF 165
           FPKD+ IQP+LVIVYFGGND++   P+     VPL  F +NM KI +H+  LS  TR IF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 166 LTPPPINSEQLKQLMPGLEKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDWL- 225
           LTPPP+N +Q++ +     K R+ E    YA+ L+++C+++ V+ +D++ AIQ ++DWL 
Sbjct: 121 LTPPPMNEKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWLN 180

Query: 226 TFFRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEF 264
           + F DG+HF  K S     EI  ++   +W P+L  +++P+EF
Sbjct: 181 SCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEF 223

BLAST of MC05g_new0388 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.3e-58
Identity = 111/227 (48.90%), Postives = 156/227 (68.72%), Query Frame = 0

Query: 46  MVGPVRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKV 105
           MVGP RP+ V+FGSSIVQ SF   GWGA ++ +YAR+AD+ILRGY GW+S +AL+VVD+V
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 106 FPKDSTIQPALVIVYFGGNDA----SINFPNSVPLDRFTDNMTKIVDHIKCLSTTTRSIF 165
           FPKD+ +QP+LVIVYFGGND+    S      VPL  + DNM KI  H++ LS  TR IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 166 LTPPPINSEQLKQ-LMPGL-EKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKEDW 225
           L+ PP++  +++Q   P L E +RT +  + Y+DA +++C++LG+E VDLF   Q  +DW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 226 LTF-FRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEFED 266
            T  F DG+H + +GS+    EI  ++ +  W P+L  ++MP EF D
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFAD 227

BLAST of MC05g_new0388 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.7e-34
Identity = 76/226 (33.63%), Postives = 125/226 (55.31%), Query Frame = 0

Query: 50  VRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFPKD 109
           +RP+ V+FG SI   SF   GWG+ +A+ Y+R+ADV++RGY G+++  AL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 110 STIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIFLTPP 169
           S+  P    ++FG NDA++    S    VP++ +TDN+ KIV H+K  S T   + +TPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 170 PINSEQLKQLMPGL-------EKLRTMENLELYADALIDICKKLGVEYVDLFRAIQNKED 229
           PI+    +     +       E  RT E   +YA   + + ++LG+  V+L+  +Q   D
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 230 W-LTFFRDGVHFNEKGSRAAFDEIKNMINKKNWTPNLDARAMPIEF 264
           W   +  DG+H   +G+   FDE+  +  ++ W   L    MP +F
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVF-REAW---LSPEEMPFDF 222

BLAST of MC05g_new0388 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 8.6e-31
Identity = 78/212 (36.79%), Postives = 119/212 (56.13%), Query Frame = 0

Query: 50  VRPKFVIFGSSIVQFSFDDKGWGADIANLYARQADVILRGYAGWSSDQALKVVDKVFP-- 109
           +R K  +FG SI + SF D GWGA +A+L  R+AD++LRGY+G+++  ALKVV++VFP  
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 110 -KDSTIQPALVIVYFGGNDASINFPNS----VPLDRFTDNMTKIVDHIKCLSTTTRSIFL 169
            +D    PA V V+FG NDA +    S    VPL  +  N+  IV  +K     T  I +
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 170 TPPPINSEQLKQLMPGLEKL-----RTMENLELYADALIDICKKLGVEYVDLFRAIQNKE 229
           TPPPI+ E+ +   P +E       RT E   LYA A I + ++  +   DL+  +Q   
Sbjct: 122 TPPPID-EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIP 181

Query: 230 DWLT-FFRDGVHFNEKGSRAAFDEIKNMINKK 249
           +W T    DG+H +  G++  F+E+   + ++
Sbjct: 182 NWQTECLWDGLHLSRVGNKVVFEEVAKKLKEE 212