MC05g1563 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1563 vs. ExPASy Swiss-Prot
Match: Q96328 (Phosphoinositide phosphatase SAC8 OS=Arabidopsis thaliana OX=3702 GN=SAC8 PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.4e-243
Identity = 400/590 (67.80%), Postives = 487/590 (82.54%), Query Frame = 0

Query: 7   SCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNATKISTIY 66
           S  RFKLYDQ EL EF D++VV+ +E    GFS+NR DGNI+PL+ +  SG+ T++STIY
Sbjct: 6   STSRFKLYDQFELLEFPDKYVVKPIESPEEGFSVNRRDGNIKPLDENASSGSPTRVSTIY 65

Query: 67  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEAYF 126
           GV GTIRL+AG Y+LVITSR+EVGNFLG P+F+VT+MKFL C+EAL+ +T+QEK+DE YF
Sbjct: 66  GVGGTIRLLAGTYLLVITSREEVGNFLGLPIFRVTAMKFLPCNEALRFATAQEKKDETYF 125

Query: 127 LTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLIE 186
            TLL+ +ETTPGLY+SY+TD+TLNLQRRCK  EGW  KP+WKQADPR++WN +LL DLIE
Sbjct: 126 RTLLQALETTPGLYFSYETDLTLNLQRRCKLAEGWNRKPMWKQADPRYVWNWHLLEDLIE 185

Query: 187 FKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFIE 246
            KLD FIIP+LQG++Q  +LKLK SPA ++++SRRCTRRLGTRMWRRGANLEGD ANF+E
Sbjct: 186 CKLDGFIIPILQGSYQVAELKLKNSPAVVSIMSRRCTRRLGTRMWRRGANLEGDAANFVE 245

Query: 247 TEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQRY 306
           +EQ+VE  GFK SLLQ+RGSIPLLWEQIVDLSYKP LKI  HEET KVV+RHF DL QRY
Sbjct: 246 SEQIVEINGFKFSLLQVRGSIPLLWEQIVDLSYKPRLKINKHEETPKVVQRHFHDLCQRY 305

Query: 307 GETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLLYDQISE 366
           GE +AVDLTD+HG+EG LS A+A EM+ LP+VRYV FDFH +CG++ F+NL +LY+QI +
Sbjct: 306 GEIMAVDLTDQHGDEGALSKAYATEMEKLPDVRYVSFDFHQVCGTTNFDNLGVLYEQIGD 365

Query: 367 DFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLLTSA 426
           +FE QGY L+D + NIL+EQKGVIRSNCIDCLDRTNVTQ F+ QK+L  QLQRIG+  S 
Sbjct: 366 EFEKQGYFLVDADENILEEQKGVIRSNCIDCLDRTNVTQSFMGQKSLNLQLQRIGVCDST 425

Query: 427 ECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMSAISRY 486
           ECI+T ++DYTKFRT W EQGDE+SL+YAGT+ALKGDLVRYGKQT  G IKDG+SA+SRY
Sbjct: 426 ECISTFEDDYTKFRTIWAEQGDEVSLQYAGTYALKGDLVRYGKQTMTGAIKDGLSAMSRY 485

Query: 487 YLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASALVVGGL 546
           YLNNF DGVRQ + DLI G Y +  H PS  Q  G +         S+LPVASAL++GG+
Sbjct: 486 YLNNFQDGVRQDALDLISGRYTVGTHSPSQLQPIGSQP--------SFLPVASALLIGGV 545

Query: 547 TLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSL 597
           T+TS T+ QAGRN QQ+++S LWAGVTAGV+A++KANGR   SRPRLC L
Sbjct: 546 TVTSFTIHQAGRNTQQYLASALWAGVTAGVVAMIKANGRHLTSRPRLCHL 587

BLAST of MC05g1563 vs. ExPASy Swiss-Prot
Match: Q9C5G5 (Phosphoinositide phosphatase SAC7 OS=Arabidopsis thaliana OX=3702 GN=SAC7 PE=2 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 4.2e-147
Identity = 265/596 (44.46%), Postives = 396/596 (66.44%), Query Frame = 0

Query: 10  RFKLYDQLELHEFQDRFVVRSVE-LASRGFSINRGDGNIEPLN--CDTCSGNATKISTIY 69
           R KL+ +L L EF D++++   +   S    I+R D +++ ++   ++ S    KI +I+
Sbjct: 7   RNKLHSRLRLWEFPDQYIIEPADGSGSSCLDISRVDASMKLIDQVPESNSVRVPKIRSIF 66

Query: 70  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEAYF 129
           GV G ++L+AG Y++V+T  + VG+FLG P+F+VT++K L CD +LK S  ++K+ E  F
Sbjct: 67  GVVGMLKLLAGSYLVVVTESERVGSFLGHPIFKVTTLKVLPCDHSLKNSPEEQKKMETEF 126

Query: 130 LTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLIE 189
             LL   E T GLY+SY+ ++TL+ QR  +  +   + PLW+QA+PRF+WN  +L  LI+
Sbjct: 127 SKLLSVAEKTTGLYFSYEVNLTLSSQRLHEMGDESKSLPLWRQAEPRFLWNNYMLEVLID 186

Query: 190 FKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFIE 249
            KLD+F++P++QG+F + +  +      +TL++RRCTRR GTRMWRRGA+L+G  ANF+E
Sbjct: 187 NKLDQFLLPVIQGSFNSFETAIGRDIVDITLIARRCTRRNGTRMWRRGADLDGYVANFVE 246

Query: 250 TEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQRY 309
           TEQ+V+  G+ +S +Q+RGS+P +WEQ+VDL+YKP  +IV  EE  ++ ERHF DL ++Y
Sbjct: 247 TEQIVQMNGYTSSFVQVRGSMPFMWEQVVDLTYKPKFEIVQPEEAKRIAERHFLDLRKKY 306

Query: 310 GETIAVDLTDKHGEEGQLSAAFAAEMQNL--PNVRYVPFDFHHICGSSKFENLQLLYDQI 369
           G  +AVDL +K G EG+L   +A  MQ++   ++RY+ FDFH ICG   FE L +LY+QI
Sbjct: 307 GSVLAVDLVNKQGGEGRLCEKYATVMQHITGDDIRYLHFDFHQICGHIHFERLSILYEQI 366

Query: 370 SEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLLT 429
               E  GY L++ +G  ++EQ GV+RSNCIDCLDRTNVTQ  + +K L  QL+RIG+  
Sbjct: 367 EGFLEKNGYFLLNEKGEKMKEQLGVVRSNCIDCLDRTNVTQSMIGRKMLEVQLKRIGVFG 426

Query: 430 SAECITT---LDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMS 489
           + E I++    DE Y   +  W   GDEIS++Y+GT ALKGD VRYG +T  GV+KDG S
Sbjct: 427 AEETISSHLNFDEHY---KILWANHGDEISIQYSGTPALKGDFVRYGHRTAHGVLKDGWS 486

Query: 490 AISRYYLNNFHDGVRQASFDLICGHYNI-IKHGPSPF-QLNGFESLSVSIISYSYLPVAS 549
           ++ RYYLNNF DG +Q + DL+ GHY + +    +P  Q  G E++       +  PVA 
Sbjct: 487 SLRRYYLNNFADGTKQDAIDLLQGHYIVAVSRDMAPVPQKGGLEAV-------ANFPVAL 546

Query: 550 ALVVGGLTLTSLTLQQAGRN--AQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRL 594
            +V+      +++L+Q G +   +    S+LW G+  G+ A+V+ANGR FC+RPRL
Sbjct: 547 FVVLMSFWFATMSLKQTGSDYKHKHLFFSLLWTGICVGMAALVRANGRIFCNRPRL 592

BLAST of MC05g1563 vs. ExPASy Swiss-Prot
Match: Q7X911 (Phosphoinositide phosphatase SAC6 OS=Arabidopsis thaliana OX=3702 GN=SAC6 PE=2 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 3.5e-146
Identity = 256/592 (43.24%), Postives = 394/592 (66.55%), Query Frame = 0

Query: 10  RFKLYDQLELHEFQDRFVVRSVELASRG-FSINRGDGNIEPLN--CDTCSGNATKISTIY 69
           R K++  L L EF D++V+   + +S     I+R DG+++ ++   +  S    KI +I+
Sbjct: 4   RLKIHSGLRLWEFPDQYVIEPTDGSSASCLDISRLDGSMKLIDQVAECNSLRVPKIRSIF 63

Query: 70  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEA-Y 129
           GV G ++L+AG Y++V+T  + VG+FLG P++++ S+KFL CD +L+    ++K+ E   
Sbjct: 64  GVVGMLKLLAGSYLVVVTESESVGSFLGHPIYKINSLKFLPCDHSLENPHEEQKKMETDD 123

Query: 130 FLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLI 189
           +  LL   E T GLY+SY+ ++TL  QR     +     PLW+QA+PRF+WN  +L  LI
Sbjct: 124 YSRLLSVAERTTGLYFSYEINLTLTAQRLHDLGDESKLLPLWRQAEPRFLWNNYMLEVLI 183

Query: 190 EFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFI 249
           + KLD+F++P++QG+F + Q  +      +TL++RRC+RR GTRMWRRGA+ +G  ANF+
Sbjct: 184 DNKLDQFLLPVIQGSFHSFQTAIGRDIVDITLIARRCSRRNGTRMWRRGADPDGYVANFV 243

Query: 250 ETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQR 309
           ETEQ+V   G+ +S +QIRGS+P +WEQIVDL+YKP  +IV  EE +++ ERHF DL ++
Sbjct: 244 ETEQIVRMNGYTSSFVQIRGSMPFMWEQIVDLTYKPKFEIVQPEEAARIAERHFLDLRKK 303

Query: 310 YGETIAVDLTDKHGEEGQLSAAFAAEMQNL--PNVRYVPFDFHHICGSSKFENLQLLYDQ 369
           YG  +AVDL +KHG EG+LS  FA  MQ++   +VRY+ FDFHHICG   FE L +LY+Q
Sbjct: 304 YGSVLAVDLVNKHGGEGRLSERFAGAMQHITGDDVRYLHFDFHHICGHIHFERLAILYEQ 363

Query: 370 ISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLL 429
           + +  E  GY L++ +G  ++EQ G++R+NCIDCLDRTNVTQ  + +K L  QL+RIG+ 
Sbjct: 364 MEDFLEKNGYFLLNEKGEKMKEQLGIVRTNCIDCLDRTNVTQSMIGRKLLELQLKRIGVF 423

Query: 430 TSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMSAI 489
            + E I +       ++  W   GD+IS++Y+GT ALKGD VRYG++T  GV++DG +A+
Sbjct: 424 GAEETIRSHQNFDECYKILWANHGDDISIQYSGTPALKGDFVRYGQRTIQGVLQDGWNAL 483

Query: 490 SRYYLNNFHDGVRQASFDLICGHYNI-IKHGPSPF-QLNGFESLSVSIISYSYLPVASAL 549
           +RYYLNNF DG +Q + DL+ GHY + +    +P  +  G E++       +  PVA  +
Sbjct: 484 ARYYLNNFADGTKQDAIDLVQGHYIVAVSRDMAPVPRKRGLEAV-------ANFPVALTV 543

Query: 550 VVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRL 594
           ++      +++++Q G   +  + S++WAG++  V A+V+ANGR FC+RP L
Sbjct: 544 ILISFWFATMSVKQVGSGYKHLLFSLVWAGISVAVAALVRANGRIFCNRPSL 588

BLAST of MC05g1563 vs. ExPASy Swiss-Prot
Match: Q6GM29 (Phosphatidylinositol-3-phosphatase SAC1 OS=Xenopus laevis OX=8355 GN=sacm1l PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.7e-92
Identity = 205/594 (34.51%), Postives = 320/594 (53.87%), Query Frame = 0

Query: 14  YDQLELHEFQDRFVVRSVELASRG-FSINRGDGNIEPLNCDTCSGN-----ATKISTIYG 73
           Y+ L+LH   ++F V + +       +I+R    +      T SG      +     IYG
Sbjct: 5   YENLKLHITPEKFYVEACDPGVEDVLTIDRVSTEV------TLSGKKDIPPSAITRAIYG 64

Query: 74  VAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEAYFL 133
           + GTIRLVAG+Y++VIT RK+VG+ L   +++ T    +S  + +   T  + +D   FL
Sbjct: 65  ILGTIRLVAGMYLIVITRRKKVGDLLNHSIWKATDFDIISYKKTMLHLTDSQLQDNKAFL 124

Query: 134 TLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDL-IE 193
            +L  V +  G Y+S   D+T  LQR    +  +    L ++AD RF+WN NLL +   +
Sbjct: 125 GMLSHVLSVDGFYFSVSYDLTHTLQRLANTSPEFQEMSLLERADQRFVWNGNLLREFSAQ 184

Query: 194 FKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFIE 253
            ++ +F IP++ G        +        L+SRR   R G R + RG + EG  ANF+E
Sbjct: 185 PEIQKFAIPVVHGFIAIHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVE 244

Query: 254 TEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPH---LKIVNHEETSKVVERHFFDLS 313
           TEQ+V + G KAS +Q RGSIP  W Q  +L YKP     K VNH +     +RHF    
Sbjct: 245 TEQIVHYNGNKASFVQTRGSIPFYWSQRPNLKYKPKPQISKAVNHMDG---FQRHFDSQV 304

Query: 314 QRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPN--VRYVPFDFHHICGSSKFENLQLLY 373
             YG+ + ++L ++ G E  L   F+  +  L N  VRY+ FDFH  C   +++ LQ+L 
Sbjct: 305 ISYGKQVVLNLVNQKGSEKPLEQEFSQMVSGLGNGMVRYIAFDFHKECSRMRWDRLQILV 364

Query: 374 DQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIG 433
           +Q++E  +  GY L+D EG ++ +Q G+ RSNC+DCLDRTNV Q  LA+++L  QLQR+G
Sbjct: 365 EQVAETQDEFGYFLVDTEGKVVSQQDGIFRSNCMDCLDRTNVVQSLLARRSLQYQLQRLG 424

Query: 434 LLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMS 493
           +L   + I    +    ++ +W +  +  + +YAGT ALK D  R GK+T  G++ DG +
Sbjct: 425 VLHVGQRIEEQIQFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWN 484

Query: 494 AISRYYLNNFHDGVRQASFDLICGHYNIIK-HGPSPFQLNGFESLSVSIISYSYLPVASA 553
           ++ RYY NNF DG RQ S DL  G+Y++ + +  SP  +            + +L +   
Sbjct: 485 SLIRYYKNNFSDGFRQDSIDLFLGNYSVEEAYSTSPLHIQ---------TDWKFLALPII 544

Query: 554 LVVGGLTLTSLTLQQAGRNAQQFMSSVL-WAGVTAGVMAVVKANGRQFCSRPRL 594
           +VV   ++  + L  AG    + ++ VL W   + G  A++  NG+ F   P+L
Sbjct: 545 MVV-AFSMCIICLLMAGDTWTETLAYVLFWGTASIGTGAIIMYNGKDFVDAPKL 579

BLAST of MC05g1563 vs. ExPASy Swiss-Prot
Match: A6QL88 (Phosphatidylinositol-3-phosphatase SAC1 OS=Bos taurus OX=9913 GN=SACM1L PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 8.5e-92
Identity = 208/599 (34.72%), Postives = 321/599 (53.59%), Query Frame = 0

Query: 14  YDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT-----------KI 73
           Y++L+LH   ++F V + +           DG  + L  D  S   T             
Sbjct: 6   YERLKLHVTPEKFYVEACD-----------DGADDVLIIDRVSTEVTLSVKKDIPPSAVT 65

Query: 74  STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRD 133
             I+G+ GTI LVAG Y++VIT +K++G F    +++ T    LS  + +   T  + +D
Sbjct: 66  RPIFGILGTIHLVAGNYLIVITKKKKIGEFFNHVIWKATDFDVLSYKKTMLHLTDIQLQD 125

Query: 134 EAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLV 193
              FL ++  V +  G Y+S   D+T  LQR    +  +    L ++AD RF+WN +LL 
Sbjct: 126 NKTFLAMMNHVLSMDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLR 185

Query: 194 DL-IEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDT 253
           +L  + ++  F +P+L G        +        L+SRR   R G R + RG + EG  
Sbjct: 186 ELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHA 245

Query: 254 ANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKP---HLKIVNHEETSKVVERH 313
           ANF+ETEQ+V + G +AS +Q RGSIPL W Q  +L YKP     K+ NH +     +RH
Sbjct: 246 ANFVETEQIVHYNGSRASFVQTRGSIPLYWSQRPNLKYKPLPLINKVANHMDG---FQRH 305

Query: 314 FFDLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPN--VRYVPFDFHHICGSSKFEN 373
           F      YG+ + ++L ++ G E  L  AFA  + +L N  +RY+ FDFH  C + +++ 
Sbjct: 306 FDSQIIIYGKQVIINLVNQKGSEKPLEQAFATMVSSLGNGMIRYIAFDFHKECKNMRWDR 365

Query: 374 LQLLYDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQ 433
           L +L DQ++E  +   Y L+DP G +L  Q+GV RSNC+DCLDRTNV Q  LA+++L  Q
Sbjct: 366 LSILLDQVAEMQDELSYFLVDPAGVVLSTQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQ 425

Query: 434 LQRIGLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVI 493
           LQR+G+L   + +   DE    ++ +W +  +  + +YAGT ALK D  R GK+T  G+I
Sbjct: 426 LQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLI 485

Query: 494 KDGMSAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSV-SIISYSYL 553
            DG +++ RYY NNF DG RQ S DL  G+Y++        +L     LSV   + +  L
Sbjct: 486 MDGWNSLIRYYKNNFSDGFRQDSIDLFLGNYSV-------DELESHSPLSVPRDLKFLAL 545

Query: 554 PVASALVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTA-GVMAVVKANGRQFCSRPRL 594
           P+   ++V   ++  + L  AG    + ++ VL+ GV + G   ++  NG+ F   PRL
Sbjct: 546 PI---IMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRL 580

BLAST of MC05g1563 vs. NCBI nr
Match: XP_022145619.1 (phosphoinositide phosphatase SAC8 [Momordica charantia])

HSP 1 Score: 1175 bits (3039), Expect = 0.0
Identity = 586/597 (98.16%), Postives = 588/597 (98.49%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT
Sbjct: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK
Sbjct: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL
Sbjct: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           LVDLIEFKLDEFIIPLLQG+FQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD
Sbjct: 181 LVDLIEFKLDEFIIPLLQGSFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF
Sbjct: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL
Sbjct: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI
Sbjct: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM
Sbjct: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAISRYYLNNFHDGVRQ + DLICGHYNIIKHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAISRYYLNNFHDGVRQDALDLICGHYNIIKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. NCBI nr
Match: XP_038905243.1 (phosphoinositide phosphatase SAC8 isoform X1 [Benincasa hispida])

HSP 1 Score: 1084 bits (2804), Expect = 0.0
Identity = 535/598 (89.46%), Postives = 566/598 (94.65%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDT-CSGNA 60
           M+G SSSCERFKLYDQLEL+EFQDRFVV+SVE  SRGFSINRGDGNIEPL+CDT C GNA
Sbjct: 1   MEGESSSCERFKLYDQLELYEFQDRFVVKSVEFPSRGFSINRGDGNIEPLDCDTTCFGNA 60

Query: 61  TKISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQE 120
           TK+STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFL CD ALKLSTSQE
Sbjct: 61  TKMSTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLPCDAALKLSTSQE 120

Query: 121 KRDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKN 180
           K+DEAYFLTLLKTVETTPGLYYSYQTD+TLNLQRRCKF EGWTA+PLWKQADPRF+WNKN
Sbjct: 121 KKDEAYFLTLLKTVETTPGLYYSYQTDLTLNLQRRCKFAEGWTARPLWKQADPRFVWNKN 180

Query: 181 LLVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEG 240
           LLV+LIEFKLDEFIIPLLQG+FQAVQLKLKESP QLTL+SRRCTRRLGTRMWRRGANLEG
Sbjct: 181 LLVELIEFKLDEFIIPLLQGSFQAVQLKLKESPTQLTLISRRCTRRLGTRMWRRGANLEG 240

Query: 241 DTANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHF 300
           DTANFIETEQLVEH G KASLLQIRGSIPLLWEQIVDLSYKPHLKIVN E++SKVV+ HF
Sbjct: 241 DTANFIETEQLVEHEGLKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNDEKSSKVVQHHF 300

Query: 301 FDLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQL 360
           FDLSQRYGE IAVDLTDKHGEEGQLSAAFAAEMQ LP VRYVPFDFHHICG++KFENLQL
Sbjct: 301 FDLSQRYGEIIAVDLTDKHGEEGQLSAAFAAEMQKLPTVRYVPFDFHHICGTTKFENLQL 360

Query: 361 LYDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQR 420
           LYDQISEDFENQGYLLIDPEGNIL+EQKG+IR+NCIDCLDRTNVTQCFLAQ++LTKQLQR
Sbjct: 361 LYDQISEDFENQGYLLIDPEGNILREQKGIIRANCIDCLDRTNVTQCFLAQRSLTKQLQR 420

Query: 421 IGLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDG 480
           +GLLTSAECIT  +EDYTKFRT W EQGDEISL+YAGTHALKGDLVRYGKQTF+GVIKDG
Sbjct: 421 VGLLTSAECITKCNEDYTKFRTLWAEQGDEISLQYAGTHALKGDLVRYGKQTFSGVIKDG 480

Query: 481 MSAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVAS 540
           MSAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVAS
Sbjct: 481 MSAITRYYLNNFHDGVRQDAIDLICGRYSINKHGPSPFQLNGFESLS-------YLPVAS 540

Query: 541 ALVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
            LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANG+QFCSRPRLCSLR
Sbjct: 541 VLVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGKQFCSRPRLCSLR 591

BLAST of MC05g1563 vs. NCBI nr
Match: XP_011651855.1 (phosphoinositide phosphatase SAC8 isoform X1 [Cucumis sativus])

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 536/597 (89.78%), Postives = 561/597 (93.97%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+G SSSCERFKLYDQLELHEFQDRFVV+SVE   RGFSINRGDGNIEPL+CDT  G+AT
Sbjct: 1   MEGESSSCERFKLYDQLELHEFQDRFVVKSVEFPDRGFSINRGDGNIEPLDCDTGFGDAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGV GTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFL CDEALKLSTSQEK
Sbjct: 61  KVSTIYGVVGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLPCDEALKLSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFL+LLKTV TTPGLYYSYQTDITLNLQRRCKF EGWTAKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLSLLKTVVTTPGLYYSYQTDITLNLQRRCKFAEGWTAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           LV+LIE KLDEF+IPLLQG+FQAVQLKLKES  QLTL+SRRCTRRLGTRMWRRGANLEGD
Sbjct: 181 LVELIELKLDEFVIPLLQGSFQAVQLKLKESLVQLTLISRRCTRRLGTRMWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEHGG KASLLQIRGSIPLLWEQIVDLSYKP LKI+N E++SKVVERHFF
Sbjct: 241 TANFIETEQLVEHGGLKASLLQIRGSIPLLWEQIVDLSYKPQLKILNDEKSSKVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DLSQRYGE IAVDLTDKHGEEGQLSAAFAAEMQ LPNVRYVPFDFHH CG++KFENLQLL
Sbjct: 301 DLSQRYGEIIAVDLTDKHGEEGQLSAAFAAEMQKLPNVRYVPFDFHHTCGTAKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQK+LT QLQRI
Sbjct: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKSLTNQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           GLLTSAECIT+  EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGKQTF+GVIKDGM
Sbjct: 421 GLLTSAECITSFSEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKQTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGRYSINKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANG+QFCSRPRLC LR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGKQFCSRPRLCGLR 590

BLAST of MC05g1563 vs. NCBI nr
Match: XP_022945768.1 (phosphoinositide phosphatase SAC8 isoform X2 [Cucurbita moschata] >XP_022945774.1 phosphoinositide phosphatase SAC8 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1080 bits (2793), Expect = 0.0
Identity = 531/597 (88.94%), Postives = 563/597 (94.30%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+ GSSSCERFKLYDQLELHEFQDRFVVRSVE  +RGFSINRGDG+IEPLNCDTC G+AT
Sbjct: 1   MECGSSSCERFKLYDQLELHEFQDRFVVRSVEFPNRGFSINRGDGDIEPLNCDTCFGSAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKF+ CD+ALK STSQEK
Sbjct: 61  KVSTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFVPCDQALKRSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFLTLLK VETTPGLYYSYQTDITLNLQRRCKF EGW AKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLTLLKIVETTPGLYYSYQTDITLNLQRRCKFAEGWAAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           +V+LIEFKLDEFIIP+LQG+FQAVQLKLKESP QLTL+SRRCTRRLGTR+WRRGANLEGD
Sbjct: 181 MVELIEFKLDEFIIPVLQGSFQAVQLKLKESPVQLTLISRRCTRRLGTRLWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH GFKASLLQIRGSIPLLWEQIVDLSYKPHL+IVNHE++S+VVERHFF
Sbjct: 241 TANFIETEQLVEHEGFKASLLQIRGSIPLLWEQIVDLSYKPHLQIVNHEKSSEVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DL+QRYGETIAVDLT+KHGEEGQLSAAFAAEMQ LPN RYVPFDFHH+CGSSKFENLQLL
Sbjct: 301 DLAQRYGETIAVDLTEKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHVCGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YD+ISEDFENQGYLLIDPEGNILQEQ+G+IRSNCIDCLDRTNVTQ FLAQK+LTKQLQRI
Sbjct: 361 YDKISEDFENQGYLLIDPEGNILQEQRGIIRSNCIDCLDRTNVTQSFLAQKSLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           G L S EC T  +EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGK TF+GVIKDGM
Sbjct: 421 GALESDECFTKFNEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKLTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGLYSINKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. NCBI nr
Match: XP_008442398.1 (PREDICTED: phosphoinositide phosphatase SAC8 [Cucumis melo])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 534/597 (89.45%), Postives = 560/597 (93.80%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+G SSSCERFKLYDQLELHEFQDRFVV+SVE  +RGFSINRGDGNIEPL+CDT  G+AT
Sbjct: 1   MEGESSSCERFKLYDQLELHEFQDRFVVKSVEFPNRGFSINRGDGNIEPLDCDTGFGDAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGV GTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFL CDEALKLSTSQEK
Sbjct: 61  KVSTIYGVVGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLPCDEALKLSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFL+LLKTV TTPGLYYSYQTDITLNLQRRCKF EGWTAKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLSLLKTVVTTPGLYYSYQTDITLNLQRRCKFAEGWTAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           LV+LIEFKLDEF+IPLLQG+FQ  QLKLKESP QLTL+SRRCTRRLGTRMWRRGANLEGD
Sbjct: 181 LVELIEFKLDEFVIPLLQGSFQTEQLKLKESPVQLTLISRRCTRRLGTRMWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH G KASLLQIRGSIPLLWEQIVDLSYKP LKI+N E++SKVVERHFF
Sbjct: 241 TANFIETEQLVEHKGLKASLLQIRGSIPLLWEQIVDLSYKPQLKILNDEKSSKVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DLSQRYGE IAVDLTDKHGEEGQLSAAFAAEMQ LPN RYVPFDFHH CG++KFENLQLL
Sbjct: 301 DLSQRYGEIIAVDLTDKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHTCGTTKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQK+LT QLQRI
Sbjct: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKSLTNQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           GLLTSAECIT  +EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGKQTF+GVIKDGM
Sbjct: 421 GLLTSAECITNFNEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKQTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I K GPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGRYSINKQGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANG+QFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGKQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. ExPASy TrEMBL
Match: A0A6J1CVS8 (phosphoinositide phosphatase SAC8 OS=Momordica charantia OX=3673 GN=LOC111015020 PE=4 SV=1)

HSP 1 Score: 1175 bits (3039), Expect = 0.0
Identity = 586/597 (98.16%), Postives = 588/597 (98.49%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT
Sbjct: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK
Sbjct: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL
Sbjct: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           LVDLIEFKLDEFIIPLLQG+FQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD
Sbjct: 181 LVDLIEFKLDEFIIPLLQGSFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF
Sbjct: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL
Sbjct: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI
Sbjct: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM
Sbjct: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAISRYYLNNFHDGVRQ + DLICGHYNIIKHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAISRYYLNNFHDGVRQDALDLICGHYNIIKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. ExPASy TrEMBL
Match: A0A6J1G1U9 (phosphoinositide phosphatase SAC8 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449896 PE=4 SV=1)

HSP 1 Score: 1080 bits (2793), Expect = 0.0
Identity = 531/597 (88.94%), Postives = 563/597 (94.30%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+ GSSSCERFKLYDQLELHEFQDRFVVRSVE  +RGFSINRGDG+IEPLNCDTC G+AT
Sbjct: 1   MECGSSSCERFKLYDQLELHEFQDRFVVRSVEFPNRGFSINRGDGDIEPLNCDTCFGSAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKF+ CD+ALK STSQEK
Sbjct: 61  KVSTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFVPCDQALKRSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFLTLLK VETTPGLYYSYQTDITLNLQRRCKF EGW AKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLTLLKIVETTPGLYYSYQTDITLNLQRRCKFAEGWAAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           +V+LIEFKLDEFIIP+LQG+FQAVQLKLKESP QLTL+SRRCTRRLGTR+WRRGANLEGD
Sbjct: 181 MVELIEFKLDEFIIPVLQGSFQAVQLKLKESPVQLTLISRRCTRRLGTRLWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH GFKASLLQIRGSIPLLWEQIVDLSYKPHL+IVNHE++S+VVERHFF
Sbjct: 241 TANFIETEQLVEHEGFKASLLQIRGSIPLLWEQIVDLSYKPHLQIVNHEKSSEVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DL+QRYGETIAVDLT+KHGEEGQLSAAFAAEMQ LPN RYVPFDFHH+CGSSKFENLQLL
Sbjct: 301 DLAQRYGETIAVDLTEKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHVCGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YD+ISEDFENQGYLLIDPEGNILQEQ+G+IRSNCIDCLDRTNVTQ FLAQK+LTKQLQRI
Sbjct: 361 YDKISEDFENQGYLLIDPEGNILQEQRGIIRSNCIDCLDRTNVTQSFLAQKSLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           G L S EC T  +EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGK TF+GVIKDGM
Sbjct: 421 GALESDECFTKFNEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKLTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGLYSINKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. ExPASy TrEMBL
Match: A0A1S3B547 (phosphoinositide phosphatase SAC8 OS=Cucumis melo OX=3656 GN=LOC103486277 PE=4 SV=1)

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 534/597 (89.45%), Postives = 560/597 (93.80%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+G SSSCERFKLYDQLELHEFQDRFVV+SVE  +RGFSINRGDGNIEPL+CDT  G+AT
Sbjct: 1   MEGESSSCERFKLYDQLELHEFQDRFVVKSVEFPNRGFSINRGDGNIEPLDCDTGFGDAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGV GTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFL CDEALKLSTSQEK
Sbjct: 61  KVSTIYGVVGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLPCDEALKLSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFL+LLKTV TTPGLYYSYQTDITLNLQRRCKF EGWTAKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLSLLKTVVTTPGLYYSYQTDITLNLQRRCKFAEGWTAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           LV+LIEFKLDEF+IPLLQG+FQ  QLKLKESP QLTL+SRRCTRRLGTRMWRRGANLEGD
Sbjct: 181 LVELIEFKLDEFVIPLLQGSFQTEQLKLKESPVQLTLISRRCTRRLGTRMWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH G KASLLQIRGSIPLLWEQIVDLSYKP LKI+N E++SKVVERHFF
Sbjct: 241 TANFIETEQLVEHKGLKASLLQIRGSIPLLWEQIVDLSYKPQLKILNDEKSSKVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DLSQRYGE IAVDLTDKHGEEGQLSAAFAAEMQ LPN RYVPFDFHH CG++KFENLQLL
Sbjct: 301 DLSQRYGEIIAVDLTDKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHTCGTTKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQK+LT QLQRI
Sbjct: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKSLTNQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           GLLTSAECIT  +EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGKQTF+GVIKDGM
Sbjct: 421 GLLTSAECITNFNEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKQTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I K GPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGRYSINKQGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANG+QFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGKQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. ExPASy TrEMBL
Match: A0A6J1I6Y4 (phosphoinositide phosphatase SAC8 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470177 PE=4 SV=1)

HSP 1 Score: 1079 bits (2790), Expect = 0.0
Identity = 530/597 (88.78%), Postives = 564/597 (94.47%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+ GSSSCERFKLYDQLELHEFQDRFVVRSVE  +RGFSINRGDG+IEPLNCDTC G+AT
Sbjct: 1   MECGSSSCERFKLYDQLELHEFQDRFVVRSVEFPNRGFSINRGDGDIEPLNCDTCFGSAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKF+ CD+ALK STSQEK
Sbjct: 61  KVSTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFVPCDQALKRSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFLTLLK VETTPGLYYSYQTDITLNLQRRCKF EGW AKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLTLLKIVETTPGLYYSYQTDITLNLQRRCKFAEGWAAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           +V+LIEFKLDEFIIP+LQG+FQAVQLKLKESP QLTL+SRRCTRRLGTR+WRRGANLEGD
Sbjct: 181 MVELIEFKLDEFIIPVLQGSFQAVQLKLKESPVQLTLISRRCTRRLGTRLWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH GFKASLLQIRGSIPLLWEQIVDLSYKPHL+IVNHE++S+VVERHFF
Sbjct: 241 TANFIETEQLVEHEGFKASLLQIRGSIPLLWEQIVDLSYKPHLQIVNHEKSSEVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DL+QRYGETIAVDLT+KHGEEGQLSAAFAAEMQ LPN RYVPFDFHH+CGSSKFENLQLL
Sbjct: 301 DLAQRYGETIAVDLTEKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHVCGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YD+ISEDFENQGYLLIDPEGNILQEQ+G+IRSNCIDCLDRTNVTQ FLAQK+LTKQLQRI
Sbjct: 361 YDKISEDFENQGYLLIDPEGNILQEQRGIIRSNCIDCLDRTNVTQSFLAQKSLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           G+L S EC T  +EDYTKFR+ W EQGDEISLEYAGTHALKGDLVRYGK TF+GVIKDGM
Sbjct: 421 GVLGSDECFTKFNEDYTKFRSLWAEQGDEISLEYAGTHALKGDLVRYGKLTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGLYSINKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 590

BLAST of MC05g1563 vs. ExPASy TrEMBL
Match: A0A6J1G1U4 (phosphoinositide phosphatase SAC8 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449896 PE=4 SV=1)

HSP 1 Score: 1075 bits (2779), Expect = 0.0
Identity = 531/600 (88.50%), Postives = 563/600 (93.83%), Query Frame = 0

Query: 1   MDGGSSSCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNAT 60
           M+ GSSSCERFKLYDQLELHEFQDRFVVRSVE  +RGFSINRGDG+IEPLNCDTC G+AT
Sbjct: 1   MECGSSSCERFKLYDQLELHEFQDRFVVRSVEFPNRGFSINRGDGDIEPLNCDTCFGSAT 60

Query: 61  KISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEK 120
           K+STIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKF+ CD+ALK STSQEK
Sbjct: 61  KVSTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFVPCDQALKRSTSQEK 120

Query: 121 RDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNL 180
           +DEAYFLTLLK VETTPGLYYSYQTDITLNLQRRCKF EGW AKPLWKQADPRF+WNKNL
Sbjct: 121 KDEAYFLTLLKIVETTPGLYYSYQTDITLNLQRRCKFAEGWAAKPLWKQADPRFVWNKNL 180

Query: 181 LVDLIEFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGD 240
           +V+LIEFKLDEFIIP+LQG+FQAVQLKLKESP QLTL+SRRCTRRLGTR+WRRGANLEGD
Sbjct: 181 MVELIEFKLDEFIIPVLQGSFQAVQLKLKESPVQLTLISRRCTRRLGTRLWRRGANLEGD 240

Query: 241 TANFIETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFF 300
           TANFIETEQLVEH GFKASLLQIRGSIPLLWEQIVDLSYKPHL+IVNHE++S+VVERHFF
Sbjct: 241 TANFIETEQLVEHEGFKASLLQIRGSIPLLWEQIVDLSYKPHLQIVNHEKSSEVVERHFF 300

Query: 301 DLSQRYGETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLL 360
           DL+QRYGETIAVDLT+KHGEEGQLSAAFAAEMQ LPN RYVPFDFHH+CGSSKFENLQLL
Sbjct: 301 DLAQRYGETIAVDLTEKHGEEGQLSAAFAAEMQKLPNARYVPFDFHHVCGSSKFENLQLL 360

Query: 361 YDQISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRI 420
           YD+ISEDFENQGYLLIDPEGNILQEQ+G+IRSNCIDCLDRTNVTQ FLAQK+LTKQLQRI
Sbjct: 361 YDKISEDFENQGYLLIDPEGNILQEQRGIIRSNCIDCLDRTNVTQSFLAQKSLTKQLQRI 420

Query: 421 GLLTSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGM 480
           G L S EC T  +EDYTKFRT W EQGDEISLEYAGTHALKGDLVRYGK TF+GVIKDGM
Sbjct: 421 GALESDECFTKFNEDYTKFRTLWAEQGDEISLEYAGTHALKGDLVRYGKLTFSGVIKDGM 480

Query: 481 SAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASA 540
           SAI+RYYLNNFHDGVRQ + DLICG Y+I KHGPSPFQLNGFESLS       YLPVASA
Sbjct: 481 SAITRYYLNNFHDGVRQDAIDLICGLYSINKHGPSPFQLNGFESLS-------YLPVASA 540

Query: 541 LVVGGLTLTSLTLQQ---AGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 597
           LVVGGLT+TSLTLQQ   AGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR
Sbjct: 541 LVVGGLTITSLTLQQGMNAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSLR 593

BLAST of MC05g1563 vs. TAIR 10
Match: AT3G51830.1 (SAC domain-containing protein 8 )

HSP 1 Score: 842.8 bits (2176), Expect = 1.7e-244
Identity = 400/590 (67.80%), Postives = 487/590 (82.54%), Query Frame = 0

Query: 7   SCERFKLYDQLELHEFQDRFVVRSVELASRGFSINRGDGNIEPLNCDTCSGNATKISTIY 66
           S  RFKLYDQ EL EF D++VV+ +E    GFS+NR DGNI+PL+ +  SG+ T++STIY
Sbjct: 6   STSRFKLYDQFELLEFPDKYVVKPIESPEEGFSVNRRDGNIKPLDENASSGSPTRVSTIY 65

Query: 67  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEAYF 126
           GV GTIRL+AG Y+LVITSR+EVGNFLG P+F+VT+MKFL C+EAL+ +T+QEK+DE YF
Sbjct: 66  GVGGTIRLLAGTYLLVITSREEVGNFLGLPIFRVTAMKFLPCNEALRFATAQEKKDETYF 125

Query: 127 LTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLIE 186
            TLL+ +ETTPGLY+SY+TD+TLNLQRRCK  EGW  KP+WKQADPR++WN +LL DLIE
Sbjct: 126 RTLLQALETTPGLYFSYETDLTLNLQRRCKLAEGWNRKPMWKQADPRYVWNWHLLEDLIE 185

Query: 187 FKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFIE 246
            KLD FIIP+LQG++Q  +LKLK SPA ++++SRRCTRRLGTRMWRRGANLEGD ANF+E
Sbjct: 186 CKLDGFIIPILQGSYQVAELKLKNSPAVVSIMSRRCTRRLGTRMWRRGANLEGDAANFVE 245

Query: 247 TEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQRY 306
           +EQ+VE  GFK SLLQ+RGSIPLLWEQIVDLSYKP LKI  HEET KVV+RHF DL QRY
Sbjct: 246 SEQIVEINGFKFSLLQVRGSIPLLWEQIVDLSYKPRLKINKHEETPKVVQRHFHDLCQRY 305

Query: 307 GETIAVDLTDKHGEEGQLSAAFAAEMQNLPNVRYVPFDFHHICGSSKFENLQLLYDQISE 366
           GE +AVDLTD+HG+EG LS A+A EM+ LP+VRYV FDFH +CG++ F+NL +LY+QI +
Sbjct: 306 GEIMAVDLTDQHGDEGALSKAYATEMEKLPDVRYVSFDFHQVCGTTNFDNLGVLYEQIGD 365

Query: 367 DFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLLTSA 426
           +FE QGY L+D + NIL+EQKGVIRSNCIDCLDRTNVTQ F+ QK+L  QLQRIG+  S 
Sbjct: 366 EFEKQGYFLVDADENILEEQKGVIRSNCIDCLDRTNVTQSFMGQKSLNLQLQRIGVCDST 425

Query: 427 ECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMSAISRY 486
           ECI+T ++DYTKFRT W EQGDE+SL+YAGT+ALKGDLVRYGKQT  G IKDG+SA+SRY
Sbjct: 426 ECISTFEDDYTKFRTIWAEQGDEVSLQYAGTYALKGDLVRYGKQTMTGAIKDGLSAMSRY 485

Query: 487 YLNNFHDGVRQASFDLICGHYNIIKHGPSPFQLNGFESLSVSIISYSYLPVASALVVGGL 546
           YLNNF DGVRQ + DLI G Y +  H PS  Q  G +         S+LPVASAL++GG+
Sbjct: 486 YLNNFQDGVRQDALDLISGRYTVGTHSPSQLQPIGSQP--------SFLPVASALLIGGV 545

Query: 547 TLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRLCSL 597
           T+TS T+ QAGRN QQ+++S LWAGVTAGV+A++KANGR   SRPRLC L
Sbjct: 546 TVTSFTIHQAGRNTQQYLASALWAGVTAGVVAMIKANGRHLTSRPRLCHL 587

BLAST of MC05g1563 vs. TAIR 10
Match: AT3G51460.1 (Phosphoinositide phosphatase family protein )

HSP 1 Score: 523.1 bits (1346), Expect = 3.0e-148
Identity = 265/596 (44.46%), Postives = 396/596 (66.44%), Query Frame = 0

Query: 10  RFKLYDQLELHEFQDRFVVRSVE-LASRGFSINRGDGNIEPLN--CDTCSGNATKISTIY 69
           R KL+ +L L EF D++++   +   S    I+R D +++ ++   ++ S    KI +I+
Sbjct: 7   RNKLHSRLRLWEFPDQYIIEPADGSGSSCLDISRVDASMKLIDQVPESNSVRVPKIRSIF 66

Query: 70  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEAYF 129
           GV G ++L+AG Y++V+T  + VG+FLG P+F+VT++K L CD +LK S  ++K+ E  F
Sbjct: 67  GVVGMLKLLAGSYLVVVTESERVGSFLGHPIFKVTTLKVLPCDHSLKNSPEEQKKMETEF 126

Query: 130 LTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLIE 189
             LL   E T GLY+SY+ ++TL+ QR  +  +   + PLW+QA+PRF+WN  +L  LI+
Sbjct: 127 SKLLSVAEKTTGLYFSYEVNLTLSSQRLHEMGDESKSLPLWRQAEPRFLWNNYMLEVLID 186

Query: 190 FKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFIE 249
            KLD+F++P++QG+F + +  +      +TL++RRCTRR GTRMWRRGA+L+G  ANF+E
Sbjct: 187 NKLDQFLLPVIQGSFNSFETAIGRDIVDITLIARRCTRRNGTRMWRRGADLDGYVANFVE 246

Query: 250 TEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQRY 309
           TEQ+V+  G+ +S +Q+RGS+P +WEQ+VDL+YKP  +IV  EE  ++ ERHF DL ++Y
Sbjct: 247 TEQIVQMNGYTSSFVQVRGSMPFMWEQVVDLTYKPKFEIVQPEEAKRIAERHFLDLRKKY 306

Query: 310 GETIAVDLTDKHGEEGQLSAAFAAEMQNL--PNVRYVPFDFHHICGSSKFENLQLLYDQI 369
           G  +AVDL +K G EG+L   +A  MQ++   ++RY+ FDFH ICG   FE L +LY+QI
Sbjct: 307 GSVLAVDLVNKQGGEGRLCEKYATVMQHITGDDIRYLHFDFHQICGHIHFERLSILYEQI 366

Query: 370 SEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLLT 429
               E  GY L++ +G  ++EQ GV+RSNCIDCLDRTNVTQ  + +K L  QL+RIG+  
Sbjct: 367 EGFLEKNGYFLLNEKGEKMKEQLGVVRSNCIDCLDRTNVTQSMIGRKMLEVQLKRIGVFG 426

Query: 430 SAECITT---LDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMS 489
           + E I++    DE Y   +  W   GDEIS++Y+GT ALKGD VRYG +T  GV+KDG S
Sbjct: 427 AEETISSHLNFDEHY---KILWANHGDEISIQYSGTPALKGDFVRYGHRTAHGVLKDGWS 486

Query: 490 AISRYYLNNFHDGVRQASFDLICGHYNI-IKHGPSPF-QLNGFESLSVSIISYSYLPVAS 549
           ++ RYYLNNF DG +Q + DL+ GHY + +    +P  Q  G E++       +  PVA 
Sbjct: 487 SLRRYYLNNFADGTKQDAIDLLQGHYIVAVSRDMAPVPQKGGLEAV-------ANFPVAL 546

Query: 550 ALVVGGLTLTSLTLQQAGRN--AQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRL 594
            +V+      +++L+Q G +   +    S+LW G+  G+ A+V+ANGR FC+RPRL
Sbjct: 547 FVVLMSFWFATMSLKQTGSDYKHKHLFFSLLWTGICVGMAALVRANGRIFCNRPRL 592

BLAST of MC05g1563 vs. TAIR 10
Match: AT5G66020.1 (Phosphoinositide phosphatase family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 2.5e-147
Identity = 256/592 (43.24%), Postives = 394/592 (66.55%), Query Frame = 0

Query: 10  RFKLYDQLELHEFQDRFVVRSVELASRG-FSINRGDGNIEPLN--CDTCSGNATKISTIY 69
           R K++  L L EF D++V+   + +S     I+R DG+++ ++   +  S    KI +I+
Sbjct: 4   RLKIHSGLRLWEFPDQYVIEPTDGSSASCLDISRLDGSMKLIDQVAECNSLRVPKIRSIF 63

Query: 70  GVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLSTSQEKRDEA-Y 129
           GV G ++L+AG Y++V+T  + VG+FLG P++++ S+KFL CD +L+    ++K+ E   
Sbjct: 64  GVVGMLKLLAGSYLVVVTESESVGSFLGHPIYKINSLKFLPCDHSLENPHEEQKKMETDD 123

Query: 130 FLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFIWNKNLLVDLI 189
           +  LL   E T GLY+SY+ ++TL  QR     +     PLW+QA+PRF+WN  +L  LI
Sbjct: 124 YSRLLSVAERTTGLYFSYEINLTLTAQRLHDLGDESKLLPLWRQAEPRFLWNNYMLEVLI 183

Query: 190 EFKLDEFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRRGANLEGDTANFI 249
           + KLD+F++P++QG+F + Q  +      +TL++RRC+RR GTRMWRRGA+ +G  ANF+
Sbjct: 184 DNKLDQFLLPVIQGSFHSFQTAIGRDIVDITLIARRCSRRNGTRMWRRGADPDGYVANFV 243

Query: 250 ETEQLVEHGGFKASLLQIRGSIPLLWEQIVDLSYKPHLKIVNHEETSKVVERHFFDLSQR 309
           ETEQ+V   G+ +S +QIRGS+P +WEQIVDL+YKP  +IV  EE +++ ERHF DL ++
Sbjct: 244 ETEQIVRMNGYTSSFVQIRGSMPFMWEQIVDLTYKPKFEIVQPEEAARIAERHFLDLRKK 303

Query: 310 YGETIAVDLTDKHGEEGQLSAAFAAEMQNL--PNVRYVPFDFHHICGSSKFENLQLLYDQ 369
           YG  +AVDL +KHG EG+LS  FA  MQ++   +VRY+ FDFHHICG   FE L +LY+Q
Sbjct: 304 YGSVLAVDLVNKHGGEGRLSERFAGAMQHITGDDVRYLHFDFHHICGHIHFERLAILYEQ 363

Query: 370 ISEDFENQGYLLIDPEGNILQEQKGVIRSNCIDCLDRTNVTQCFLAQKTLTKQLQRIGLL 429
           + +  E  GY L++ +G  ++EQ G++R+NCIDCLDRTNVTQ  + +K L  QL+RIG+ 
Sbjct: 364 MEDFLEKNGYFLLNEKGEKMKEQLGIVRTNCIDCLDRTNVTQSMIGRKLLELQLKRIGVF 423

Query: 430 TSAECITTLDEDYTKFRTSWGEQGDEISLEYAGTHALKGDLVRYGKQTFAGVIKDGMSAI 489
            + E I +       ++  W   GD+IS++Y+GT ALKGD VRYG++T  GV++DG +A+
Sbjct: 424 GAEETIRSHQNFDECYKILWANHGDDISIQYSGTPALKGDFVRYGQRTIQGVLQDGWNAL 483

Query: 490 SRYYLNNFHDGVRQASFDLICGHYNI-IKHGPSPF-QLNGFESLSVSIISYSYLPVASAL 549
           +RYYLNNF DG +Q + DL+ GHY + +    +P  +  G E++       +  PVA  +
Sbjct: 484 ARYYLNNFADGTKQDAIDLVQGHYIVAVSRDMAPVPRKRGLEAV-------ANFPVALTV 543

Query: 550 VVGGLTLTSLTLQQAGRNAQQFMSSVLWAGVTAGVMAVVKANGRQFCSRPRL 594
           ++      +++++Q G   +  + S++WAG++  V A+V+ANGR FC+RP L
Sbjct: 544 ILISFWFATMSVKQVGSGYKHLLFSLVWAGISVAVAALVRANGRIFCNRPSL 588

BLAST of MC05g1563 vs. TAIR 10
Match: AT3G14205.1 (Phosphoinositide phosphatase family protein )

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-43
Identity = 157/562 (27.94%), Postives = 243/562 (43.24%), Query Frame = 0

Query: 56  SGNATKISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLS 115
           SG    ++T YG+ G IR +   YML+IT RK++G   G  V+ V   K ++   A  LS
Sbjct: 84  SGGLKFVTTCYGIIGFIRFLGPYYMLIITKRKKLGEICGHTVYGVAKSKIITIPHASVLS 143

Query: 116 TSQEKRDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRR-CKFTEGWTAKPLWKQADPRF 175
                +DE  +  LL TV+ T   ++SY   I   LQR      EG T        +  F
Sbjct: 144 NVAYSKDEKRYKRLLCTVDLTKDFFFSYSYHIMHTLQRNLSNNVEGHT------YYESMF 203

Query: 176 IWNKNLLVDLIEFKLD-EFIIPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRR 235
           +WN+ L   +     D  + + L+ G F+ V+L + E   +LTL+SRR     GTR  +R
Sbjct: 204 VWNEYLTRRIRNNVKDCMWTVALVYGFFKQVKLSVSEKNFRLTLISRRSRHYAGTRYLKR 263

Query: 236 GANLEGDTANFIETEQLV------EHGGFKASLLQIRGSIPLLWEQIVD-LSYKPHLKIV 295
           G N +G  AN +ETEQ+V       + G  +S++Q RGSIPL W Q    L+ KP + + 
Sbjct: 264 GVNEKGRVANDVETEQIVFEEAQDGNPGRISSVVQNRGSIPLFWSQETSRLNIKPDIILS 323

Query: 296 NHEETSKVVERHFFDLSQRYGETI----AVDLTDKHGEEGQLSAAFAAEMQNL------- 355
             +   +    HF +L +RYG  I     +   +K   E  L A FA  ++ +       
Sbjct: 324 PKDPNFEATRLHFENLGRRYGNPIIILNLIKTREKRPRETILRAEFANAIRFINKGLSKE 383

Query: 356 PNVRYVPFDFHH-----------ICGSSKFENLQL---LYDQISEDFENQGY-------- 415
             +R + +D H            I G      L L    Y Q++ D   +G+        
Sbjct: 384 DRLRPLHWDLHKHSRKKGTNVLAILGRLATYALNLTSIFYCQLTPDLRGEGFQNQNPSTL 443

Query: 416 ---------------------LLI-----------DPEGNILQEQKGVIRSNCIDCLDRT 475
                                L++           D +  +   QKGV+R+NCIDCLDRT
Sbjct: 444 ENDDGECSTYDPPSKDETAPNLVVENGNDSKDAKEDQQKEVTMLQKGVLRTNCIDCLDRT 503

Query: 476 NVTQCFLAQKTLTKQLQRIGLLTSAECITTLDEDYTKFRTSWG---EQGDEISLEYAGTH 535
           NV Q         +QL  +GL  S    T +D D        G     GD ++L+Y G+ 
Sbjct: 504 NVAQYAYGLVAFGRQLHALGLTES----TNIDLDNPLAEDLMGIYETMGDTLALQYGGSA 563

Query: 536 A-LKGDLVRYGKQTFAGVIKDGMSAISRYYLNNFHDGVRQASFDLICGHYNIIKHGPSPF 540
           A  K    R G+   A   ++    + RYY N + D  +Q + ++  G++      P+ +
Sbjct: 564 AHNKIFCERRGQWRAATQSQEFFRTLQRYYSNAYMDAEKQDAINVFLGYFQPQSDKPALW 623

BLAST of MC05g1563 vs. TAIR 10
Match: AT1G22620.1 (Phosphoinositide phosphatase family protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.9e-42
Identity = 149/568 (26.23%), Postives = 242/568 (42.61%), Query Frame = 0

Query: 56  SGNATKISTIYGVAGTIRLVAGVYMLVITSRKEVGNFLGFPVFQVTSMKFLSCDEALKLS 115
           +G    ++ +YG+AG  + +   Y++++T R+++G   G  ++ +   + +S   A   S
Sbjct: 99  TGGLAFVAKVYGIAGCAKFMESYYLVLVTKRRQIGCICGHAIYAIDESQMISVPHATIQS 158

Query: 116 TSQEKRDEAYFLTLLKTVETTPGLYYSYQTDITLNLQRRCKFTEGWTAKPLWKQADPRFI 175
                + E  +  LL +V+ T   +YSY   I  +LQ+    + G    P     D  F+
Sbjct: 159 DVANSKTELRYKKLLSSVDLTKDFFYSYTYPIMQSLQKNV-LSSGEEGMPY----DNIFV 218

Query: 176 WNKNLLVDLIEFKLDEFI--IPLLQGNFQAVQLKLKESPAQLTLVSRRCTRRLGTRMWRR 235
           WN + L   I  + +  I  + L+ G+F+ ++L +      +TLVSRR     GTR  +R
Sbjct: 219 WN-SYLTQPIRSRCNNTIWTLALVHGHFKQIRLSIYGRDFSVTLVSRRSRHFAGTRYLKR 278

Query: 236 GANLEGDTANFIETEQLV---EHGGFK---ASLLQIRGSIPLLWEQIVD-LSYKPHLKIV 295
           G N  G  AN +ETEQLV   E G  K   +S++Q+RGSIPL W Q     S KP + + 
Sbjct: 279 GVNDRGRVANDVETEQLVLDDEAGSCKGKMSSVVQMRGSIPLFWSQEASRFSPKPDIFLQ 338

Query: 296 NHEETSKVVERHFFDLSQRYGETIAV----DLTDKHGEEGQLSAAFAAEMQNLPNV---- 355
            ++ T +  + HF DL  RYG  I V       +K   E  L   FA  +  L ++    
Sbjct: 339 RYDPTYESTKMHFEDLVNRYGNPIIVLNLIKTVEKRPREMVLRREFANAVGYLNSIFREE 398

Query: 356 ---RYVPFDFHHICGSSKFENLQLLYDQISEDFENQGYLLI------------------- 415
              +++ +DFH    S     L +L    SE  +  G                       
Sbjct: 399 NHLKFIHWDFHKFAKSKSANVLAVLGAVASEALDLTGLYFSGKPKIVKKKASQLSHANTA 458

Query: 416 -------------------------------DPEGNILQEQK-------------GVIRS 475
                                          + E  + Q++K             GV+R+
Sbjct: 459 REPSLRDLRAYSAELSRGESANDILSALANREKEMKLTQQKKDEGTNSSAPRYQSGVLRT 518

Query: 476 NCIDCLDRTNVTQCFLAQKTLTKQLQRIGLLTSAECITTLDEDYT---KFRTSWGEQGDE 535
           NCIDCLDRTNV Q       L +QL  +GL  + +    +D D +        +   GD 
Sbjct: 519 NCIDCLDRTNVAQYAYGLAALGRQLHAMGLSDTPK----IDPDSSIAAALMDMYQSMGDA 578

Query: 536 ISLEYAGTHALKGDL-VRYGKQTFAGVIKDGMSAISRYYLNNFHDGVRQASFDLICGHYN 537
           ++ +Y G+ A       R GK       ++ + +I RYY N + DG +Q + +L  G++ 
Sbjct: 579 LAQQYGGSAAHNTVFPERQGKWKATTQSREFLKSIKRYYSNTYTDGEKQDAINLFLGYFQ 638