MC05g1479 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1479
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC05: 18753815 .. 18754873 (-)
RNA-Seq Expression	MC05g1479
Synteny	MC05g1479

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCAATCGGTTCCTCGGGGTTCGGGCTGGGGCCCTAGATTCGGTCCGTTGTTCTGACTGTGGTGGAGAAAACGTTGAAGAATTTTGGTCGCCGCCCTCTCTGTCTCCGTTTCGCCATCGGTTTCCGGCTGCTGCGATGTACCACGATGACGCTTCCGACCCAGACCCCTCCTTCGTCCCAAGATTGCGTCGCAGCAGAAGAAACGGTGAGGATCGCGCCCCTTTTAATCGTATCATCGTTCTACGCGATCCCTCTAATGCGGGCGCTATTAGCAATAATGGTATTGGAGCTGGAGGTAATTACGAGATTTATTATGATGATGGTACTGGGTCTACCCTGCGGCCCTTACCTTCTAATATATCCGAATTCTTGATGGGATCGGGGTTCGACCGCCTTCTTAATCAATTGGCGCAGCTGGAGGTGAATGGGGTCGGCTCATTGGAACACCCACCAGCTTCAAAGGCTGCAATTGAATCTTTGCCCGTGGTTAAGATTCTTGCTAGCCATGTGCCCGTGGAATCACATTGTGCTGTCTGTAAGGAACCGTTTGAACTGGACTCTGAGGCTCGAGAAATGCCGTGTAAGCATATCTACCATTCGGATTGTATACTTCCTTGGCTTTCTATCCGGAATTCATGTCCGGTTTGTCGGCATCAGTTGCCTACCGATGGACATGGCAGTGGTCGGAGCTCCCCTGCGTCTGCAGAAGAAGTTGTTGGATTAACAATTTGGAGGCTTCCGGGTGGGGGATTTGCTGTTGGGAGGTTTACCTGGGGTAGGGGAGCTGCAGAGCACGACCTTCCTGTTGTTTACACTGAAATTGATGGTGGGTTTAGTACCACCAGCGGTATTCCAAGGAGGATTTCATGGGCGTCAAGCGGAAGGAGATCAAGTGAGGGTGGTGGTTTTCGTCGTGTTTTTCGCAATTTCTTCTCTTTTTTTGGCAGGTTTAGGAGTTCATCACATCATTCTAGTTCAGGGACTGGTTTAATGAGAAGGAGTTTCTCATCTACATTATTCAACAGACGCTCAAGGAGGAACAATCACCTTACGGCGTCC

mRNA sequence

TGCAATCGGTTCCTCGGGGTTCGGGCTGGGGCCCTAGATTCGGTCCGTTGTTCTGACTGTGGTGGAGAAAACGTTGAAGAATTTTGGTCGCCGCCCTCTCTGTCTCCGTTTCGCCATCGGTTTCCGGCTGCTGCGATGTACCACGATGACGCTTCCGACCCAGACCCCTCCTTCGTCCCAAGATTGCGTCGCAGCAGAAGAAACGGTGAGGATCGCGCCCCTTTTAATCGTATCATCGTTCTACGCGATCCCTCTAATGCGGGCGCTATTAGCAATAATGGTATTGGAGCTGGAGGTAATTACGAGATTTATTATGATGATGGTACTGGGTCTACCCTGCGGCCCTTACCTTCTAATATATCCGAATTCTTGATGGGATCGGGGTTCGACCGCCTTCTTAATCAATTGGCGCAGCTGGAGGTGAATGGGGTCGGCTCATTGGAACACCCACCAGCTTCAAAGGCTGCAATTGAATCTTTGCCCGTGGTTAAGATTCTTGCTAGCCATGTGCCCGTGGAATCACATTGTGCTGTCTGTAAGGAACCGTTTGAACTGGACTCTGAGGCTCGAGAAATGCCGTGTAAGCATATCTACCATTCGGATTGTATACTTCCTTGGCTTTCTATCCGGAATTCATGTCCGGTTTGTCGGCATCAGTTGCCTACCGATGGACATGGCAGTGGTCGGAGCTCCCCTGCGTCTGCAGAAGAAGTTGTTGGATTAACAATTTGGAGGCTTCCGGGTGGGGGATTTGCTGTTGGGAGGTTTACCTGGGGTAGGGGAGCTGCAGAGCACGACCTTCCTGTTGTTTACACTGAAATTGATGGTGGGTTTAGTACCACCAGCGGTATTCCAAGGAGGATTTCATGGGCGTCAAGCGGAAGGAGATCAAGTGAGGGTGGTGGTTTTCGTCGTGTTTTTCGCAATTTCTTCTCTTTTTTTGGCAGGTTTAGGAGTTCATCACATCATTCTAGTTCAGGGACTGGTTTAATGAGAAGGAGTTTCTCATCTACATTATTCAACAGACGCTCAAGGAGGAACAATCACCTTACGGCGTCC

Coding sequence (CDS)

TGCAATCGGTTCCTCGGGGTTCGGGCTGGGGCCCTAGATTCGGTCCGTTGTTCTGACTGTGGTGGAGAAAACGTTGAAGAATTTTGGTCGCCGCCCTCTCTGTCTCCGTTTCGCCATCGGTTTCCGGCTGCTGCGATGTACCACGATGACGCTTCCGACCCAGACCCCTCCTTCGTCCCAAGATTGCGTCGCAGCAGAAGAAACGGTGAGGATCGCGCCCCTTTTAATCGTATCATCGTTCTACGCGATCCCTCTAATGCGGGCGCTATTAGCAATAATGGTATTGGAGCTGGAGGTAATTACGAGATTTATTATGATGATGGTACTGGGTCTACCCTGCGGCCCTTACCTTCTAATATATCCGAATTCTTGATGGGATCGGGGTTCGACCGCCTTCTTAATCAATTGGCGCAGCTGGAGGTGAATGGGGTCGGCTCATTGGAACACCCACCAGCTTCAAAGGCTGCAATTGAATCTTTGCCCGTGGTTAAGATTCTTGCTAGCCATGTGCCCGTGGAATCACATTGTGCTGTCTGTAAGGAACCGTTTGAACTGGACTCTGAGGCTCGAGAAATGCCGTGTAAGCATATCTACCATTCGGATTGTATACTTCCTTGGCTTTCTATCCGGAATTCATGTCCGGTTTGTCGGCATCAGTTGCCTACCGATGGACATGGCAGTGGTCGGAGCTCCCCTGCGTCTGCAGAAGAAGTTGTTGGATTAACAATTTGGAGGCTTCCGGGTGGGGGATTTGCTGTTGGGAGGTTTACCTGGGGTAGGGGAGCTGCAGAGCACGACCTTCCTGTTGTTTACACTGAAATTGATGGTGGGTTTAGTACCACCAGCGGTATTCCAAGGAGGATTTCATGGGCGTCAAGCGGAAGGAGATCAAGTGAGGGTGGTGGTTTTCGTCGTGTTTTTCGCAATTTCTTCTCTTTTTTTGGCAGGTTTAGGAGTTCATCACATCATTCTAGTTCAGGGACTGGTTTAATGAGAAGGAGTTTCTCATCTACATTATTCAACAGACGCTCAAGGAGGAACAATCACCTTACGGCGTCC

Protein sequence

CNRFLGVRAGALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKILASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHGSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIPRRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSRRNNHLTAS

Homology

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BLAST of MC05g1479 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 181/404 (44.80%), Postives = 239/404 (59.16%), Query Frame = 0


Query: 1   CNRFLGVRAGALDSVRCSDCGGENVEEFWSPPSLSP---------FRHRFPA-------- 60
           C+RF+ V     DS+ C DC G  +E    P   +P          +HR P         
Sbjct: 11  CSRFVWVS----DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPPPSSS 70

Query: 61  ----AAMYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAG- 120
               +A  H D S P P+ V R R       +R+P N +IVLR   +A A S++ +  G 
Sbjct: 71  SSTPSASMHADNS-PTPTIVTRTR------SNRSP-NPVIVLR--GSAAAPSSDVVSEGL 130

Query: 121 --GNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVN---GVGSLEHPPAS 180
               +++YYDDGT S LRPLP +++EFL+GSGFDRLL+Q++Q+E+N    + S EHPPAS
Sbjct: 131 DRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPAS 190

Query: 181 KAAIESLPVVKILASHV--PVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRN 240
           K+AIE+LP+++I  +H+    +SHCAVCKE F L S AREMPC HIYH DCILPWL+IRN
Sbjct: 191 KSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRN 250

Query: 241 SCPVCRHQLP----TDGHGSGRSS-PASAEE----VVGLTIWRLPGGGFAVGRFTWGRGA 300
           SCPVCRH+LP    TDG G+  ++  A+AEE      GLTIWRLPGGGFAVGR   G   
Sbjct: 251 SCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGWRG 310

Query: 301 AEHDLPVVYTEIDGGFSTTSGIPRRISWAS------SGRRSSEGGGFR-RVFRNFFSFFG 351
            +  +PVVYTE+DGG      +PRR++W S       G     GGGF  R+ R F  F G
Sbjct: 311 GDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMRLFGCFSG 370

[Close]

BLAST of MC05g1479 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.3e-70
Identity = 167/349 (47.85%), Postives = 199/349 (57.02%), Query Frame = 0


Query: 1   CNRFLGV--RAGALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDPSF 60
           C RF+ V     A   V C  C G  +EE     + +        AA+    AS P+   
Sbjct: 36  CTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNST-------VAAI---PASTPEVRS 95

Query: 61  VPRLRRS---RRNGEDRAPFNRIIVLRDPSNAGA----ISNNGIGAGGN---YEIYYDDG 120
           V    RS   RR    R  FN +IVL      GA     +  G GA      YE YYDDG
Sbjct: 96  VEETHRSIIRRRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRAYEFYYDDG 155

Query: 121 TGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEV--NGVGSLEHPPASKAAIESLPVVKIL 180
           +GS LRPLP ++SE LMGSGF+RLL QL+Q+E   NG+G   +PPASK+AIESLP V+I 
Sbjct: 156 SGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEIS 215

Query: 181 ASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHG 240
             H   E++CAVC E FE   E REMPCKHI+H DCI+PWLSIRNSCPVCR +LP+D   
Sbjct: 216 DCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD--P 275

Query: 241 SGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIPR 300
             RS+    E  VG+TIWRLPGGGFAVGRF  G    E  LPVV TE+DGG   ++  PR
Sbjct: 276 IQRSN--EEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDGGGLGSNEGPR 335

Query: 301 RISW---------ASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSS 327
           RISW         + +G RS  GG  RR  R   SF  R R S   S+S
Sbjct: 336 RISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSRGSSNS 370

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BLAST of MC05g1479 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.3e-67
Identity = 158/354 (44.63%), Postives = 203/354 (57.34%), Query Frame = 0


Query: 1   CNRFLGVRAG----ALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDP 60
           C RF+ V A      + SV C  C G  +E+  +  S +      PA+      + + + 
Sbjct: 23  CTRFVSVWADQGTTTVGSVACPHCDGGFIEQI-NDSSSAATELTIPASTEVR--SINNNR 82

Query: 61  SFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGN--YEIYYDDGTGSTLR 120
             V R RRS R    R  FN +IVL+  +        G  A     +E YYDDG+GS LR
Sbjct: 83  RSVIRRRRSGR----RPSFNPVIVLQGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLR 142

Query: 121 PLPSNISEFLMGSGFDRLLNQLAQLEVN--GVGSLEHPPASKAAIESLPVVKILASHVPV 180
           PLP ++SE LMGSGF+RLL QL+Q+E +  G+G   +PPASK+AIESLP V+I   H+  
Sbjct: 143 PLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGS 202

Query: 181 ESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHGSGRSSP 240
           E++CAVC E FE ++EAREMPCKH++H DCI+PWLSIRNSCPVCR +LP++   + RS+ 
Sbjct: 203 EANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE--PNRRSNN 262

Query: 241 ASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEID-GGFSTTSGIPRRISWA 300
              +  VG+TIWRLPGGGFAVGRF       E  LPVV TE+D GG   +   PRRISW 
Sbjct: 263 NEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGGIGNSQDGPRRISWV 322

Query: 301 SS-----------GRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRS 335
            S           G  S  GG  RR+ R   S   R R +   SSS   +   S
Sbjct: 323 RSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRPNRSSSSSSAAISTSS 367

[Close]

BLAST of MC05g1479 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 89/249 (35.74%), Postives = 129/249 (51.81%), Query Frame = 0


Query: 1   CNRFLGVRAGALDS-VRCSDCGGENVEEFWSP-PSL-SPFRHRFPAAAMYHD---DASDP 60
           CNR + V      S   C  C    +EE+ +P P+  S F +  P +  +HD     SDP
Sbjct: 28  CNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLTLSDP 87

Query: 61  DPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAIS-------------------NNGI 120
             S +P L  S  +       +  I   +PS +G                      N+  
Sbjct: 88  FASLLPLLFPS--SSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLNDLR 147

Query: 121 GAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKA 180
            +G   E    +        LP+NI ++ +G G ++L+ QLA+ + N  G+   PPASK+
Sbjct: 148 SSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGT---PPASKS 207

Query: 181 AIESLPVVKILASHVPVE-SHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCP 224
           AIE+LP+V I  S++  E + CAVC + FE  +EA++MPCKH+YH DC+LPWL + NSCP
Sbjct: 208 AIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCP 267

[Close]

BLAST of MC05g1479 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.9e-28
Identity = 80/254 (31.50%), Postives = 121/254 (47.64%), Query Frame = 0


Query: 1   CNRFLGVRAGALDSVRCSDCGGENVEEFWSP-----------------PSLSPFRHRFP- 60
           CN+ + +   +     C  C    +EE+  P                 P   PF    P 
Sbjct: 26  CNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFSTLLPL 85

Query: 61  ------AAAMYHDDASDPDPSFVPRLRRSRRNGEDRA--PF----NRIIVLRDPSNAGAI 120
                 AA    D  S   PS  P+ R +++N +  A  PF    N +  LR        
Sbjct: 86  IFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLR-------- 145

Query: 121 SNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHP 180
                 +G ++E   ++        +P N  ++  G G ++L+ QLA+ + N  G+   P
Sbjct: 146 -----SSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGT---P 205

Query: 181 PASKAAIESLPVVKILASHVPVE-SHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSI 224
           PASK+AI++LP VK+    +  E + CAVC + FE  S+ ++MPCKH++H DC+LPWL +
Sbjct: 206 PASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL 263

[Close]

BLAST of MC05g1479 vs. NCBI nr
Match: XP_022145737.1 (probable E3 ubiquitin-protein ligase RHC2A [Momordica charantia])

HSP 1 Score: 625 bits (1613), Expect = 2.14e-225
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY
Sbjct: 1   MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI
Sbjct: 61  DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH
Sbjct: 121 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
           GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP
Sbjct: 181 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR
Sbjct: 241 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 300

Query: 346 RNNHLTAS 353
           RNNHLTAS
Sbjct: 301 RNNHLTAS 308

[Close]

BLAST of MC05g1479 vs. NCBI nr
Match: XP_038906308.1 (E3 ubiquitin-protein ligase RDUF1-like [Benincasa hispida])

HSP 1 Score: 578 bits (1489), Expect = 2.27e-206
Identity = 284/308 (92.21%), Postives = 295/308 (95.78%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           MYHDDASDPDPSFVPRLRRS RNGEDR+PFNRIIVLRDPSN+GAI NNGIGAGGNYEIYY
Sbjct: 1   MYHDDASDPDPSFVPRLRRSGRNGEDRSPFNRIIVLRDPSNSGAIGNNGIGAGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTG++LRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVG LE+PPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGPLENPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LASHVP+ESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLP+D H
Sbjct: 121 LASHVPMESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPSDVH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
            SGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSG+P
Sbjct: 181 ISGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGVP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           RRI+WASSGRRSSE GGFRR+FRNFFSFF RFRSSS HS SG GLMRRSFS+TLFNRRSR
Sbjct: 241 RRITWASSGRRSSESGGFRRIFRNFFSFFRRFRSSSRHSGSGHGLMRRSFSATLFNRRSR 300

Query: 346 RNNHLTAS 353
           RNN   AS
Sbjct: 301 RNNQPIAS 308

[Close]

BLAST of MC05g1479 vs. NCBI nr
Match: KAG7017281.1 (E3 ubiquitin-protein ligase RDUF1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581 bits (1498), Expect = 2.27e-206
Identity = 288/339 (84.96%), Postives = 308/339 (90.86%), Query Frame = 0


Query: 12  LDSVRCSDCG-GENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDPSFVPRLRRSRRNGE 71
           +D++  S+ G G  +    S PS+SP  HRFP AAM HDDASD DPSFVPRLRRSRRNGE
Sbjct: 55  IDAIDLSELGLGPYIR---SLPSVSPVDHRFPPAAMNHDDASDLDPSFVPRLRRSRRNGE 114

Query: 72  DRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFD 131
           DR+ FNRIIVLRDPSN+GAISNNG+G GGNYEIYYDDGTG++LR LPSNISEFLMGSG D
Sbjct: 115 DRSSFNRIIVLRDPSNSGAISNNGVGGGGNYEIYYDDGTGASLRSLPSNISEFLMGSGID 174

Query: 132 RLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKILASHVPVESHCAVCKEPFELDSEAR 191
           RLLNQLAQLEVNGVG LEHPPASKAAIESLPVVKILAS VP+ESHCAVCKEPFELDSEAR
Sbjct: 175 RLLNQLAQLEVNGVGPLEHPPASKAAIESLPVVKILASDVPLESHCAVCKEPFELDSEAR 234

Query: 192 EMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHGSGRSSPASAEEVVGLTIWRLPGGG 251
           +MPCKHIYHSDCI PWLSIRNSCPVCRHQLPTD HGSGRSSPASAEEVVGLTIWRLPGGG
Sbjct: 235 QMPCKHIYHSDCIFPWLSIRNSCPVCRHQLPTDAHGSGRSSPASAEEVVGLTIWRLPGGG 294

Query: 252 FAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIPRRISWASSGRRSSEGGGFRRVFRNF 311
           FAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSG+PR I+WASSG RS+E GGFRR+FRNF
Sbjct: 295 FAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGVPRSITWASSGIRSNESGGFRRIFRNF 354

Query: 312 FSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSRRNNH 349
           FSFFGRFRS+S  S S TGL+RRSFS+TLFNRRSRRNNH
Sbjct: 355 FSFFGRFRSTSRRSGSVTGLIRRSFSTTLFNRRSRRNNH 390

[Close]

BLAST of MC05g1479 vs. NCBI nr
Match: KAA0044102.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK25035.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 568 bits (1463), Expect = 6.02e-202
Identity = 281/324 (86.73%), Postives = 295/324 (91.05%), Query Frame = 0


Query: 30  SPPSLSPFRHRFPAAAMYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGA 89
           S PSL P  HRFPAAAMYHDDAS P+PSFVPRLRRS RNGEDR+PFNR IVLRDPSN+G 
Sbjct: 14  SLPSLYPVVHRFPAAAMYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGV 73

Query: 90  ISNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEH 149
           +SNNGIGAGGNYEI+ DDGTG++LRPLPSNISEFLMGSGFDRLLNQLA+LEVN V  LE+
Sbjct: 74  VSNNGIGAGGNYEIFCDDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLEN 133

Query: 150 PPASKAAIESLPVVKILASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSI 209
           PPASKAAIESLPVVKILASHVP+ESHCAVCKEPFELDSEAREMPCKHIYH DCILPWLSI
Sbjct: 134 PPASKAAIESLPVVKILASHVPMESHCAVCKEPFELDSEAREMPCKHIYHLDCILPWLSI 193

Query: 210 RNSCPVCRHQLPTDGHGSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPV 269
           RNSCPVCRHQLPTD H SGR+SPASAEEVVGLTIWRLPG GFAVGRFTWGRGAAEHDLPV
Sbjct: 194 RNSCPVCRHQLPTDVHSSGRNSPASAEEVVGLTIWRLPGRGFAVGRFTWGRGAAEHDLPV 253

Query: 270 VYTEIDGGFSTTSGIPRRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTG 329
           VYTEIDGGFSTTSGIPRRI+W SSGRRS E GGFRR+FRN FSF GRFRSSS HS SG G
Sbjct: 254 VYTEIDGGFSTTSGIPRRITWESSGRRSVESGGFRRLFRNLFSFLGRFRSSSRHSGSGPG 313

Query: 330 LMRRSFSSTLFNRRSRRNNHLTAS 353
            MRRSFS+ LFNRRSRRNN   AS
Sbjct: 314 SMRRSFSARLFNRRSRRNNQPIAS 337

[Close]

BLAST of MC05g1479 vs. NCBI nr
Match: XP_023526166.1 (E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560 bits (1444), Expect = 1.62e-199
Identity = 274/304 (90.13%), Postives = 287/304 (94.41%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           M HDDASD DPSFVPRLRRSRRNGEDR+ FNRIIVLRDPSN+GAISNNG+G GGNYEIYY
Sbjct: 1   MNHDDASDLDPSFVPRLRRSRRNGEDRSSFNRIIVLRDPSNSGAISNNGVGGGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTG++LR LPSNISEFLMGSG DRLLNQLAQLEVNGVG LEHPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRSLPSNISEFLMGSGIDRLLNQLAQLEVNGVGPLEHPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LAS VP+ESHCAVCKEPFELDSEAR+MPCKHIYHSDCI PWLSIRNSCPVCRHQLPTD H
Sbjct: 121 LASDVPLESHCAVCKEPFELDSEARQMPCKHIYHSDCIFPWLSIRNSCPVCRHQLPTDAH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
           GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSG+P
Sbjct: 181 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGVP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           R I+WASSG RSSE GGFRR+FRNFFSFFGRFRS+S  S S TGL+RRSFS+TLFNRRSR
Sbjct: 241 RSITWASSGIRSSESGGFRRIFRNFFSFFGRFRSTSRRSGSVTGLIRRSFSTTLFNRRSR 300

Query: 346 RNNH 349
           RNNH
Sbjct: 301 RNNH 304

[Close]

BLAST of MC05g1479 vs. ExPASy TrEMBL
Match: A0A6J1CXJ7 (probable E3 ubiquitin-protein ligase RHC2A OS=Momordica charantia OX=3673 GN=LOC111015120 PE=4 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.04e-225
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY
Sbjct: 1   MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI
Sbjct: 61  DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH
Sbjct: 121 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
           GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP
Sbjct: 181 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR
Sbjct: 241 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 300

Query: 346 RNNHLTAS 353
           RNNHLTAS
Sbjct: 301 RNNHLTAS 308

[Close]

BLAST of MC05g1479 vs. ExPASy TrEMBL
Match: A0A5A7TL92 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001550 PE=4 SV=1)

HSP 1 Score: 568 bits (1463), Expect = 2.92e-202
Identity = 281/324 (86.73%), Postives = 295/324 (91.05%), Query Frame = 0


Query: 30  SPPSLSPFRHRFPAAAMYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGA 89
           S PSL P  HRFPAAAMYHDDAS P+PSFVPRLRRS RNGEDR+PFNR IVLRDPSN+G 
Sbjct: 14  SLPSLYPVVHRFPAAAMYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGV 73

Query: 90  ISNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEH 149
           +SNNGIGAGGNYEI+ DDGTG++LRPLPSNISEFLMGSGFDRLLNQLA+LEVN V  LE+
Sbjct: 74  VSNNGIGAGGNYEIFCDDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLEN 133

Query: 150 PPASKAAIESLPVVKILASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSI 209
           PPASKAAIESLPVVKILASHVP+ESHCAVCKEPFELDSEAREMPCKHIYH DCILPWLSI
Sbjct: 134 PPASKAAIESLPVVKILASHVPMESHCAVCKEPFELDSEAREMPCKHIYHLDCILPWLSI 193

Query: 210 RNSCPVCRHQLPTDGHGSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPV 269
           RNSCPVCRHQLPTD H SGR+SPASAEEVVGLTIWRLPG GFAVGRFTWGRGAAEHDLPV
Sbjct: 194 RNSCPVCRHQLPTDVHSSGRNSPASAEEVVGLTIWRLPGRGFAVGRFTWGRGAAEHDLPV 253

Query: 270 VYTEIDGGFSTTSGIPRRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTG 329
           VYTEIDGGFSTTSGIPRRI+W SSGRRS E GGFRR+FRN FSF GRFRSSS HS SG G
Sbjct: 254 VYTEIDGGFSTTSGIPRRITWESSGRRSVESGGFRRLFRNLFSFLGRFRSSSRHSGSGPG 313

Query: 330 LMRRSFSSTLFNRRSRRNNHLTAS 353
            MRRSFS+ LFNRRSRRNN   AS
Sbjct: 314 SMRRSFSARLFNRRSRRNNQPIAS 337

[Close]

BLAST of MC05g1479 vs. ExPASy TrEMBL
Match: A0A6J1F5C6 (E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita moschata OX=3662 GN=LOC111442455 PE=4 SV=1)

HSP 1 Score: 559 bits (1441), Expect = 2.25e-199
Identity = 273/304 (89.80%), Postives = 287/304 (94.41%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           M HDDASD DPSFVPRLRRSRRNGEDR+ FNRIIVLRDPSN+GAISNNG+G GGNYEIYY
Sbjct: 1   MNHDDASDLDPSFVPRLRRSRRNGEDRSSFNRIIVLRDPSNSGAISNNGVGGGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTG++LR LPSNISEFLMGSG DRLLNQLAQLEVNGVG LEHPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRSLPSNISEFLMGSGIDRLLNQLAQLEVNGVGPLEHPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LAS VP+ESHCAVCKEPFELDSEAR+MPCKHIYHSDCI PWLSIRNSCPVCRHQLPTD H
Sbjct: 121 LASDVPLESHCAVCKEPFELDSEARQMPCKHIYHSDCIFPWLSIRNSCPVCRHQLPTDAH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
           GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSG+P
Sbjct: 181 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGVP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           R I+WASSG RS+E GGFRR+FRNFFSFFGRFRS+S  S S TGL+RRSFS+TLFNRRSR
Sbjct: 241 RSITWASSGIRSNESGGFRRIFRNFFSFFGRFRSTSRRSGSVTGLIRRSFSTTLFNRRSR 300

Query: 346 RNNH 349
           RNNH
Sbjct: 301 RNNH 304

[Close]

BLAST of MC05g1479 vs. ExPASy TrEMBL
Match: A0A6J1J964 (E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita maxima OX=3661 GN=LOC111482444 PE=4 SV=1)

HSP 1 Score: 558 bits (1437), Expect = 9.15e-199
Identity = 273/304 (89.80%), Postives = 286/304 (94.08%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           M HDDASD DPSFVPRLRRSRRNGEDR+ FNRIIVLRDPSN+GAISNNG+G GGNYEIYY
Sbjct: 1   MNHDDASDLDPSFVPRLRRSRRNGEDRSSFNRIIVLRDPSNSGAISNNGVGGGGNYEIYY 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTG++LR LPSNISEFLMGSG DRLLNQLAQLEVNGVG LEHPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRSLPSNISEFLMGSGIDRLLNQLAQLEVNGVGPLEHPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LASHVP+ESHCAVCKEPFELDSEAR+MPCKHIYHSDCI PWLSIRNSCPVCRHQLPTD H
Sbjct: 121 LASHVPLESHCAVCKEPFELDSEARQMPCKHIYHSDCICPWLSIRNSCPVCRHQLPTDVH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
           GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTT G+P
Sbjct: 181 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTIGVP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           R I+WASSG RSSE GGFR +FRNFFSFFGRFRS+S  S S TGL+RRSFS+TLFNRRSR
Sbjct: 241 RSITWASSGIRSSESGGFRSIFRNFFSFFGRFRSTSRRSGSVTGLIRRSFSTTLFNRRSR 300

Query: 346 RNNH 349
           RNNH
Sbjct: 301 RNNH 304

[Close]

BLAST of MC05g1479 vs. ExPASy TrEMBL
Match: A0A1S3B6J2 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103486374 PE=4 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 2.39e-194
Identity = 269/308 (87.34%), Postives = 283/308 (91.88%), Query Frame = 0


Query: 46  MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 105
           MYHDDAS P+PSFVPRLRRS RNGEDR+PFNR IVLRDPSN+G +SNNGIGAGGNYEI+ 
Sbjct: 1   MYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGVVSNNGIGAGGNYEIFC 60

Query: 106 DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 165
           DDGTG++LRPLPSNISEFLMGSGFDRLLNQLA+LEVN V  LE+PPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLENPPASKAAIESLPVVKI 120

Query: 166 LASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGH 225
           LASHVP+ESHCAVCKEPFELDSEAREMPCKHIYH DCILPWLSIRNSCPVCRHQLPTD H
Sbjct: 121 LASHVPMESHCAVCKEPFELDSEAREMPCKHIYHLDCILPWLSIRNSCPVCRHQLPTDVH 180

Query: 226 GSGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 285
            SGR+SPASAEEVVGLTIWRLPG GFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP
Sbjct: 181 SSGRNSPASAEEVVGLTIWRLPGRGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIP 240

Query: 286 RRISWASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRSFSSTLFNRRSR 345
           RRI+W SSGRRS E GGFRR+FRN FSF GRFRSSS HS SG G MRRSFS+ LFNRRSR
Sbjct: 241 RRITWESSGRRSVESGGFRRLFRNLFSFLGRFRSSSRHSGSGPGSMRRSFSARLFNRRSR 300

Query: 346 RNNHLTAS 353
           RNN   AS
Sbjct: 301 RNNQPIAS 308

[Close]

BLAST of MC05g1479 vs. TAIR 10
Match: AT2G39720.1 (RING-H2 finger C2A )

HSP 1 Score: 271.6 bits (693), Expect = 9.2e-73
Identity = 181/404 (44.80%), Postives = 239/404 (59.16%), Query Frame = 0


Query: 1   CNRFLGVRAGALDSVRCSDCGGENVEEFWSPPSLSP---------FRHRFPA-------- 60
           C+RF+ V     DS+ C DC G  +E    P   +P          +HR P         
Sbjct: 11  CSRFVWVS----DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPPPSSS 70

Query: 61  ----AAMYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAG- 120
               +A  H D S P P+ V R R       +R+P N +IVLR   +A A S++ +  G 
Sbjct: 71  SSTPSASMHADNS-PTPTIVTRTR------SNRSP-NPVIVLR--GSAAAPSSDVVSEGL 130

Query: 121 --GNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVN---GVGSLEHPPAS 180
               +++YYDDGT S LRPLP +++EFL+GSGFDRLL+Q++Q+E+N    + S EHPPAS
Sbjct: 131 DRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPAS 190

Query: 181 KAAIESLPVVKILASHV--PVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRN 240
           K+AIE+LP+++I  +H+    +SHCAVCKE F L S AREMPC HIYH DCILPWL+IRN
Sbjct: 191 KSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRN 250

Query: 241 SCPVCRHQLP----TDGHGSGRSS-PASAEE----VVGLTIWRLPGGGFAVGRFTWGRGA 300
           SCPVCRH+LP    TDG G+  ++  A+AEE      GLTIWRLPGGGFAVGR   G   
Sbjct: 251 SCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGRIPGGWRG 310

Query: 301 AEHDLPVVYTEIDGGFSTTSGIPRRISWAS------SGRRSSEGGGFR-RVFRNFFSFFG 351
            +  +PVVYTE+DGG      +PRR++W S       G     GGGF  R+ R F  F G
Sbjct: 311 GDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMRLFGCFSG 370

[Close]

BLAST of MC05g1479 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.6e-71
Identity = 167/349 (47.85%), Postives = 199/349 (57.02%), Query Frame = 0


Query: 1   CNRFLGV--RAGALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDPSF 60
           C RF+ V     A   V C  C G  +EE     + +        AA+    AS P+   
Sbjct: 36  CTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNST-------VAAI---PASTPEVRS 95

Query: 61  VPRLRRS---RRNGEDRAPFNRIIVLRDPSNAGA----ISNNGIGAGGN---YEIYYDDG 120
           V    RS   RR    R  FN +IVL      GA     +  G GA      YE YYDDG
Sbjct: 96  VEETHRSIIRRRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRAYEFYYDDG 155

Query: 121 TGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEV--NGVGSLEHPPASKAAIESLPVVKIL 180
           +GS LRPLP ++SE LMGSGF+RLL QL+Q+E   NG+G   +PPASK+AIESLP V+I 
Sbjct: 156 SGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEIS 215

Query: 181 ASHVPVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHG 240
             H   E++CAVC E FE   E REMPCKHI+H DCI+PWLSIRNSCPVCR +LP+D   
Sbjct: 216 DCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD--P 275

Query: 241 SGRSSPASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEIDGGFSTTSGIPR 300
             RS+    E  VG+TIWRLPGGGFAVGRF  G    E  LPVV TE+DGG   ++  PR
Sbjct: 276 IQRSN--EEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDGGGLGSNEGPR 335

Query: 301 RISW---------ASSGRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSS 327
           RISW         + +G RS  GG  RR  R   SF  R R S   S+S
Sbjct: 336 RISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSRGSSNS 370

[Close]

BLAST of MC05g1479 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.3e-68
Identity = 158/354 (44.63%), Postives = 203/354 (57.34%), Query Frame = 0


Query: 1   CNRFLGVRAG----ALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDP 60
           C RF+ V A      + SV C  C G  +E+  +  S +      PA+      + + + 
Sbjct: 23  CTRFVSVWADQGTTTVGSVACPHCDGGFIEQI-NDSSSAATELTIPASTEVR--SINNNR 82

Query: 61  SFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGN--YEIYYDDGTGSTLR 120
             V R RRS R    R  FN +IVL+  +        G  A     +E YYDDG+GS LR
Sbjct: 83  RSVIRRRRSGR----RPSFNPVIVLQGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLR 142

Query: 121 PLPSNISEFLMGSGFDRLLNQLAQLEVN--GVGSLEHPPASKAAIESLPVVKILASHVPV 180
           PLP ++SE LMGSGF+RLL QL+Q+E +  G+G   +PPASK+AIESLP V+I   H+  
Sbjct: 143 PLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGS 202

Query: 181 ESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTDGHGSGRSSP 240
           E++CAVC E FE ++EAREMPCKH++H DCI+PWLSIRNSCPVCR +LP++   + RS+ 
Sbjct: 203 EANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE--PNRRSNN 262

Query: 241 ASAEEVVGLTIWRLPGGGFAVGRFTWGRGAAEHDLPVVYTEID-GGFSTTSGIPRRISWA 300
              +  VG+TIWRLPGGGFAVGRF       E  LPVV TE+D GG   +   PRRISW 
Sbjct: 263 NEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGGIGNSQDGPRRISWV 322

Query: 301 SS-----------GRRSSEGGGFRRVFRNFFSFFGRFRSSSHHSSSGTGLMRRS 335
            S           G  S  GG  RR+ R   S   R R +   SSS   +   S
Sbjct: 323 RSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRPNRSSSSSSAAISTSS 367

[Close]

BLAST of MC05g1479 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.8e-29
Identity = 80/254 (31.50%), Postives = 121/254 (47.64%), Query Frame = 0


Query: 1   CNRFLGVRAGALDSVRCSDCGGENVEEFWSP-----------------PSLSPFRHRFP- 60
           CN+ + +   +     C  C    +EE+  P                 P   PF    P 
Sbjct: 26  CNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFSTLLPL 85

Query: 61  ------AAAMYHDDASDPDPSFVPRLRRSRRNGEDRA--PF----NRIIVLRDPSNAGAI 120
                 AA    D  S   PS  P+ R +++N +  A  PF    N +  LR        
Sbjct: 86  IFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLR-------- 145

Query: 121 SNNGIGAGGNYEIYYDDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHP 180
                 +G ++E   ++        +P N  ++  G G ++L+ QLA+ + N  G+   P
Sbjct: 146 -----SSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGT---P 205

Query: 181 PASKAAIESLPVVKILASHVPVE-SHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSI 224
           PASK+AI++LP VK+    +  E + CAVC + FE  S+ ++MPCKH++H DC+LPWL +
Sbjct: 206 PASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL 263

[Close]

BLAST of MC05g1479 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-27
Identity = 85/248 (34.27%), Postives = 120/248 (48.39%), Query Frame = 0


Query: 1   CNRFLGVRAGALDSVRCSDCGGENVEEFWSPPSLSPFRHRFPAAAMYHDDASDPDPSFVP 60
           C R + +R  A D+V CS CGG  VEE    PS +           + D   DP    + 
Sbjct: 13  CQRAVWLR--ARDAV-CSYCGGGFVEEIDIGPSRA-----------HRDVERDPTFDLME 72

Query: 61  RLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGA----GGNYEIYYDDGTGSTLRPL 120
                 R+      ++R I  R   +AG+ S + +      GG        G  S++   
Sbjct: 73  AFSAFMRSRLAERSYDREISGR-LGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEAF 132

Query: 121 ------------PSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVK 180
                        +N  ++  G G + L+ QL+    +G      PPA K++I++LP +K
Sbjct: 133 VNGAAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALPTIK 192

Query: 181 ILASHV-PVESHCAVCKEPFELDSEAREMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTD 232
           I   H+   +SHC VCK+ FEL SEA++MPC HIYHSDCI+PWL   NSCPVCR +LP+ 
Sbjct: 193 ITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR 241

The following BLAST results are available for this feature:

BLAST of MC05g1479 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22283	1.3e-71	44.80	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SNB6	9.3e-70	47.85	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q940T5	3.3e-67	44.63	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
P0CH30	1.1e-30	35.74	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	3.9e-28	31.50	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of MC05g1479 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145737.1	2.14e-225	100.00	probable E3 ubiquitin-protein ligase RHC2A [Momordica charantia]	[more]
XP_038906308.1	2.27e-206	92.21	E3 ubiquitin-protein ligase RDUF1-like [Benincasa hispida]	[more]
KAG7017281.1	2.27e-206	84.96	E3 ubiquitin-protein ligase RDUF1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0044102.1	6.02e-202	86.73	E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK25035.1 E3...	[more]
XP_023526166.1	1.62e-199	90.13	E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC05g1479 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXJ7	1.04e-225	100.00	probable E3 ubiquitin-protein ligase RHC2A OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5A7TL92	2.92e-202	86.73	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1F5C6	2.25e-199	89.80	E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1J964	9.15e-199	89.80	E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita maxima OX=3661 GN=LOC1114824...	[more]
A0A1S3B6J2	2.39e-194	87.34	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103486374 P...	[more]

BLAST of MC05g1479 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39720.1	9.2e-73	44.80	RING-H2 finger C2A	[more]
AT3G46620.1	6.6e-71	47.85	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G59550.1	2.3e-68	44.63	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G19950.1	2.8e-29	31.50	RING/U-box superfamily protein	[more]
AT3G56580.2	1.5e-27	34.27	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 176..216 e-value: 2.5E-7 score: 40.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 175..217 e-value: 1.1E-10 score: 41.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 176..217 score: 12.179465
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 142..223 e-value: 5.2E-20 score: 72.9
IPR010543	Domain of unknown function DUF1117	PFAM	PF06547	DUF1117	coord: 239..329 e-value: 4.4E-37 score: 127.1
None	No IPR available	PANTHER	PTHR15710:SF61	E3 UBIQUITIN-PROTEIN LIGASE RHC2A-RELATED	coord: 1..305
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 1..305
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 176..217 e-value: 2.5956E-23 score: 88.914
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 156..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1479.1	MC05g1479.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity