MC03g_new0222 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0222
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protein NUCLEAR FUSION DEFECTIVE 4-like
Location	MC03: 9622824 .. 9623540 (-)
RNA-Seq Expression	MC03g_new0222
Synteny	MC03g_new0222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGGCGCAAGCTCACCCTACCTGTTCGGAACATATTCAGAAGTTATAAAGACCCAATTCAATTACAGTCAAACCCAAGTTAACACCGTGGGATTCGCCAAAGATCTCGGCTCCAACCTCGGAGTTTTCGCCGGAGTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCATCCCTCAACTTCTTCAGCTTCTTCATAATTTGGCTCTCTGTCACTCACCGGATTGCCATGCCCCAGTTCTGGCAAATGTTCCTCTACATCTGCTTGGCTACTAATGCACAAAATTTCGCCAACACCGCGGTTATGGTCATCAGCGTCGAAAACTTCCCAGACCGACGAGGGATTATTCTTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCTGTCTTAACTCAAATCTATTTAGCCATTTACGGCGACAAAGATGCATCTGATGTGCTTCTTCTACTTGCATGGTTCCCATCCATTATGTCTATGGTGCTTTTGTTTTCAATTCGAACCATCAAAGCTCGGAAACATCCGGAGGAGCTCAAAGTGTTTTACCACTTGCTTTACGCATCCATAATTCTGGCTGTGTTTATCTTCATTTTGACGTTAACTCAGAAGCATATTGTTTTCTCTCATGTTGGCTATGTTGGTGGCGTCGTTGTGGTTATAGCATTTCTGTGTCTGCCCCTCTTGATCGCCATTAAAGAATAA

mRNA sequence

ATGATCGGCGCAAGCTCACCCTACCTGTTCGGAACATATTCAGAAGTTATAAAGACCCAATTCAATTACAGTCAAACCCAAGTTAACACCGTGGGATTCGCCAAAGATCTCGGCTCCAACCTCGGAGTTTTCGCCGGAGTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCATCCCTCAACTTCTTCAGCTTCTTCATAATTTGGCTCTCTGTCACTCACCGGATTGCCATGCCCCAGTTCTGGCAAATGTTCCTCTACATCTGCTTGGCTACTAATGCACAAAATTTCGCCAACACCGCGGTTATGGTCATCAGCGTCGAAAACTTCCCAGACCGACGAGGGATTATTCTTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCTGTCTTAACTCAAATCTATTTAGCCATTTACGGCGACAAAGATGCATCTGATGTGCTTCTTCTACTTGCATGGTTCCCATCCATTATGTCTATGGTGCTTTTGTTTTCAATTCGAACCATCAAAGCTCGGAAACATCCGGAGGAGCTCAAAGTGTTTTACCACTTGCTTTACGCATCCATAATTCTGGCTGTGTTTATCTTCATTTTGACGTTAACTCAGAAGCATATTGTTTTCTCTCATGTTGGCTATGTTGGTGGCGTCGTTGTGGTTATAGCATTTCTGTGTCTGCCCCTCTTGATCGCCATTAAAGAATAA

Coding sequence (CDS)

ATGATCGGCGCAAGCTCACCCTACCTGTTCGGAACATATTCAGAAGTTATAAAGACCCAATTCAATTACAGTCAAACCCAAGTTAACACCGTGGGATTCGCCAAAGATCTCGGCTCCAACCTCGGAGTTTTCGCCGGAGTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCATCCCTCAACTTCTTCAGCTTCTTCATAATTTGGCTCTCTGTCACTCACCGGATTGCCATGCCCCAGTTCTGGCAAATGTTCCTCTACATCTGCTTGGCTACTAATGCACAAAATTTCGCCAACACCGCGGTTATGGTCATCAGCGTCGAAAACTTCCCAGACCGACGAGGGATTATTCTTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCTGTCTTAACTCAAATCTATTTAGCCATTTACGGCGACAAAGATGCATCTGATGTGCTTCTTCTACTTGCATGGTTCCCATCCATTATGTCTATGGTGCTTTTGTTTTCAATTCGAACCATCAAAGCTCGGAAACATCCGGAGGAGCTCAAAGTGTTTTACCACTTGCTTTACGCATCCATAATTCTGGCTGTGTTTATCTTCATTTTGACGTTAACTCAGAAGCATATTGTTTTCTCTCATGTTGGCTATGTTGGTGGCGTCGTTGTGGTTATAGCATTTCTGTGTCTGCCCCTCTTGATCGCCATTAAAGAATAA

Protein sequence

MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVVGSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGIILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHPEELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE

Homology

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BLAST of MC03g_new0222 vs. NCBI nr
Match: XP_022152848.1 (uncharacterized membrane protein YMR155W-like [Momordica charantia])

HSP 1 Score: 397 bits (1020), Expect = 3.18e-133
Identity = 203/238 (85.29%), Postives = 223/238 (93.70%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA SPYLFGTYS+VIKTQF+YSQTQVNT+GF+KDLGSNLGVFAG+LAEVAPPWVLFVV
Sbjct: 31  MIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLAEVAPPWVLFVV 90

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GSSLNFFSF +IWLSVTHRIA P+FWQMFLY+ LA NAQNFANTAVMVISV+NFPDRRGI
Sbjct: 91  GSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVKNFPDRRGI 150

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           I+GLLKGFVGLGGAVLTQIYLAIYG +D SDVLLLL+WFPS++S+VLLFSIRTI+AR HP
Sbjct: 151 IIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRTIRARXHP 210

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EELKVFYHLLYASIILA+ I I+TLTQKHIVFSH  Y+G V VV+A LCLPLLIAIKE
Sbjct: 211 EELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSVAVVVALLCLPLLIAIKE 268

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BLAST of MC03g_new0222 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 363 bits (932), Expect = 5.60e-120
Identity = 182/238 (76.47%), Postives = 210/238 (88.24%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S YLFGTYS+++KTQF+Y QTQVNT+GFAKDLGSN GVFAG+LAEVAP WVLF+V
Sbjct: 32  MIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVFAGLLAEVAPSWVLFLV 91

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GSSLNFFS+F+IWLSVT RIA PQFW M LYICLA N+QNFANTAV+V SV+NFP+RRGI
Sbjct: 92  GSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNFANTAVLVTSVKNFPERRGI 151

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA+LTQ+YLAIYG ++ + V+LLLAWFPS++S V + SIRTIK RKHP
Sbjct: 152 ILGLLKGFVGIGGAILTQMYLAIYGHENQASVILLLAWFPSVISFVFVLSIRTIKVRKHP 211

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EELKVFYHLLY SIILA++I  LTLTQK  VFSH GYVGG  V++  LCLPLLIAI+E
Sbjct: 212 EELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGASVIVCLLCLPLLIAIRE 269

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BLAST of MC03g_new0222 vs. NCBI nr
Match: XP_008464767.1 (PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo] >TYK17154.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 338 bits (866), Expect = 9.34e-110
Identity = 164/238 (68.91%), Postives = 200/238 (84.03%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S Y+FGTYS+VIKTQFNYSQT++NT+GFAKDLGSNLGVFAG L E+APPWVL VV
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G+ LNF+S+F+IWLSVT RIA PQ WQMF YICLA N+QNFANTAV+V SV NFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA+LTQ +LA+YG ++ S+++LLL+WFP+++S+    SIRTI  R+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EEL+V YHLLY SIILA+F+  LT+TQK   FS  GY  G  V++  L +PLLIA++E
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVRE 265

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BLAST of MC03g_new0222 vs. NCBI nr
Match: XP_004147309.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8652749.1 hypothetical protein Csa_013078 [Cucumis sativus])

HSP 1 Score: 337 bits (865), Expect = 1.28e-109
Identity = 164/238 (68.91%), Postives = 201/238 (84.45%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S Y+FGTYS+ IKTQF+YSQT++NT+GFAKDLGSNLGVFAG+L E+APPWVLFVV
Sbjct: 27  MIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLLGEIAPPWVLFVV 86

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GS LNFFS+F+IWLS+THRIA PQ WQMF+YICLA N+QNFANTAV+V SV NFPDRRGI
Sbjct: 87  GSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNFANTAVLVTSVRNFPDRRGI 146

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA++TQ YLA++G  + + ++LLLAWFP+++S +   SIRTI  R+HP
Sbjct: 147 ILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPTLISSLFFLSIRTINMRRHP 206

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EEL+V YHLLY SIILA+F+  LT++QK   FS  GY  G  V+I  L +PLLIA++E
Sbjct: 207 EELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGAAVIIGLLLMPLLIAVRE 264

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BLAST of MC03g_new0222 vs. NCBI nr
Match: KAG6583552.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333 bits (855), Expect = 2.16e-108
Identity = 164/238 (68.91%), Postives = 205/238 (86.13%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIG  SPYLFGTYS+++KT+F+Y+QTQ+NT+GFAKDLGSNLGVFAG+ AEVAPPW+LF+V
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G S NFFS+F+IWLSV++ ++ PQ W MF+YI + +N+QNF NTAVMV SV NFPD+RGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVSKPQLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVGLGGA+LTQI LAIYG++++ D+LLLL+W PSI+  +    IRTIKA+KHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKAQKHP 210

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           +ELKVFYHLLY SI +AVF+  LT+TQ++I FS  GYVGGV V++  +CLPLLIAIKE
Sbjct: 211 QELKVFYHLLYVSISIAVFLLFLTITQRNIAFSQAGYVGGVAVIVVLICLPLLIAIKE 268

[Close]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL
Match: A0A6J1DG00 (uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=LOC111020471 PE=4 SV=1)

HSP 1 Score: 397 bits (1020), Expect = 1.54e-133
Identity = 203/238 (85.29%), Postives = 223/238 (93.70%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA SPYLFGTYS+VIKTQF+YSQTQVNT+GF+KDLGSNLGVFAG+LAEVAPPWVLFVV
Sbjct: 31  MIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLAEVAPPWVLFVV 90

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GSSLNFFSF +IWLSVTHRIA P+FWQMFLY+ LA NAQNFANTAVMVISV+NFPDRRGI
Sbjct: 91  GSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVKNFPDRRGI 150

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           I+GLLKGFVGLGGAVLTQIYLAIYG +D SDVLLLL+WFPS++S+VLLFSIRTI+AR HP
Sbjct: 151 IIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRTIRARXHP 210

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EELKVFYHLLYASIILA+ I I+TLTQKHIVFSH  Y+G V VV+A LCLPLLIAIKE
Sbjct: 211 EELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSVAVVVALLCLPLLIAIKE 268

[Close]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 363 bits (932), Expect = 2.71e-120
Identity = 182/238 (76.47%), Postives = 210/238 (88.24%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S YLFGTYS+++KTQF+Y QTQVNT+GFAKDLGSN GVFAG+LAEVAP WVLF+V
Sbjct: 32  MIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVFAGLLAEVAPSWVLFLV 91

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GSSLNFFS+F+IWLSVT RIA PQFW M LYICLA N+QNFANTAV+V SV+NFP+RRGI
Sbjct: 92  GSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNFANTAVLVTSVKNFPERRGI 151

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA+LTQ+YLAIYG ++ + V+LLLAWFPS++S V + SIRTIK RKHP
Sbjct: 152 ILGLLKGFVGIGGAILTQMYLAIYGHENQASVILLLAWFPSVISFVFVLSIRTIKVRKHP 211

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EELKVFYHLLY SIILA++I  LTLTQK  VFSH GYVGG  V++  LCLPLLIAI+E
Sbjct: 212 EELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGASVIVCLLCLPLLIAIRE 269

[Close]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL
Match: A0A5D3D1P8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00170 PE=4 SV=1)

HSP 1 Score: 338 bits (866), Expect = 4.52e-110
Identity = 164/238 (68.91%), Postives = 200/238 (84.03%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S Y+FGTYS+VIKTQFNYSQT++NT+GFAKDLGSNLGVFAG L E+APPWVL VV
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G+ LNF+S+F+IWLSVT RIA PQ WQMF YICLA N+QNFANTAV+V SV NFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA+LTQ +LA+YG ++ S+++LLL+WFP+++S+    SIRTI  R+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EEL+V YHLLY SIILA+F+  LT+TQK   FS  GY  G  V++  L +PLLIA++E
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVRE 265

[Close]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL
Match: A0A1S3CMR5 (uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=4 SV=1)

HSP 1 Score: 338 bits (866), Expect = 4.52e-110
Identity = 164/238 (68.91%), Postives = 200/238 (84.03%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIGA S Y+FGTYS+VIKTQFNYSQT++NT+GFAKDLGSNLGVFAG L E+APPWVL VV
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G+ LNF+S+F+IWLSVT RIA PQ WQMF YICLA N+QNFANTAV+V SV NFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVG+GGA+LTQ +LA+YG ++ S+++LLL+WFP+++S+    SIRTI  R+HP
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           EEL+V YHLLY SIILA+F+  LT+TQK   FS  GY  G  V++  L +PLLIA++E
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVRE 265

[Close]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL
Match: A0A6J1HLW5 (uncharacterized protein LOC111464774 OS=Cucurbita moschata OX=3662 GN=LOC111464774 PE=4 SV=1)

HSP 1 Score: 333 bits (853), Expect = 1.92e-108
Identity = 164/238 (68.91%), Postives = 204/238 (85.71%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           MIG  SPYLFGTYS+++KT+F+Y+QTQ+NT+GFAKDLGSNLGVFAG+ AEVAPPW+LF+V
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G S NFFS+F+IWLSV++ ++ PQ W MF+YI + +N+QNF NTAVMV SV NFPD+RGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVSKPQLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           ILGLLKGFVGLGGA+LTQI LAIYG++++ D+LLLL+W PSI+  +    IRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGNQNSMDLLLLLSWLPSIVCSLCFLPIRTIKARKHP 210

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 238
           +ELKVFYHLLY SI +AVF+  LT+TQ++IVFS  GY  GV V++  +CLPLLIAIKE
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIVFSQAGYASGVAVIVVLICLPLLIAIKE 268

[Close]

BLAST of MC03g_new0222 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 3.8e-62
Identity = 113/238 (47.48%), Postives = 165/238 (69.33%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           M  A + YLFGTYS+ IK+   Y QT +N +GF KDLG+N+GV +G++AEV P W +  +
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           GS++NF  +F+IWL+VT ++A P+ WQM LYIC+  N+QNFANT  +V  V+NFP+ RG+
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           +LGLLKG+VGL GA+ TQ+Y AIYG  D+  ++LL+AW P+ +S+V ++ IR  K  +  
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYG-HDSKSLILLIAWLPAAVSLVFVYLIREKKVVRQR 207

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 239
            EL VFY  LY SI LA+F+  + + +K + FS   Y     +  A L +PL +++K+
Sbjct: 208 NELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQ 264

[Close]

BLAST of MC03g_new0222 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 6.0e-60
Identity = 112/238 (47.06%), Postives = 163/238 (68.49%), Query Frame = 0


Query: 1   MIGASSPYLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVV 60
           M  A + Y+FG YS  IK    Y QT +N + F KDLG+N+GV AG+L EV PPW + ++
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  GSSLNFFSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGI 120
           G+ LNFF +F+IWL+VT RI+ PQ W M LYIC+  N+Q+FANT  +V  V+NFP+ RG+
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 ILGLLKGFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARKHP 180
           +LG+LKG+VGL GA++TQ+Y A YG+ D  +++L++ W P+I+S   L +IR +K ++  
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYGE-DTKELILMIGWLPAIVSFAFLRTIRIMKVKRQT 211

Query: 181 EELKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAIKE 239
            ELKVFY+ LY S+ LA F+ ++ +  K   F+   + G   VVI  L LP+++ I E
Sbjct: 212 NELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILE 268

[Close]

BLAST of MC03g_new0222 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.0e-35
Identity = 85/231 (36.80%), Postives = 134/231 (58.01%), Query Frame = 0


Query: 8   YLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVVGSSLNFF 67
           YLFG+ S VIK+  NY+Q Q++ +G AKDLG ++G  AG L+E+ P W   +VGS  N  
Sbjct: 34  YLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLSEILPLWAALLVGSVQNLV 93

Query: 68  SFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGIILGLLKG 127
            +  +WL VT R  +   W M + I +  N + + NTA +V  V+NFP  RG ++G+LKG
Sbjct: 94  GYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVSGVQNFPKSRGPVVGILKG 153

Query: 128 FVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARK--HPEELKV 187
           F GLGGA+L+Q+Y  I+   D + ++ ++A  PS++ + L+F IR +   +     +   
Sbjct: 154 FAGLGGAILSQVYTMIH-SSDRASLIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATS 213

Query: 188 FYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAI 237
           F  +    I+LA ++  + L +  I  SH   +   VV+ A L +P+ I I
Sbjct: 214 FTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAILLVPIFIPI 263

[Close]

BLAST of MC03g_new0222 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 6.7e-35
Identity = 83/231 (35.93%), Postives = 135/231 (58.44%), Query Frame = 0


Query: 8   YLFGTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVVGSSLNFF 67
           YLFG+ S VIK+  NY+Q +++ +G AKDLG ++G  AG L+E+ P W   +VG+  N  
Sbjct: 34  YLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLSEILPLWAALLVGAVQNLI 93

Query: 68  SFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGIILGLLKG 127
            +  +WL VT R  +   W M + I +  N + + NT  +V  V+NFP  RG ++G+LKG
Sbjct: 94  GYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGILKG 153

Query: 128 FVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTIKARK--HPEELKV 187
           F GLGGA+++QIY  I+    AS ++L++A  P+++ + L+F IR +   K   P +   
Sbjct: 154 FAGLGGAIISQIYTMIHSSNPAS-LILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTDGAS 213

Query: 188 FYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLIAI 237
           F  +    ++LA ++  + L Q  +V SH       +V+   L +P+L+ I
Sbjct: 214 FTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVILVVPILVPI 263

[Close]

BLAST of MC03g_new0222 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 8.2e-33
Identity = 80/232 (34.48%), Postives = 138/232 (59.48%), Query Frame = 0


Query: 8   YLF-GTYSEVIKTQFNYSQTQVNTVGFAKDLGSNLGVFAGVLAEVAPPWVLFVVGSSLNF 67
           YLF G+ S  IKT   Y+Q Q+  +G AK+LG  +G  +G L+EV+P WV+ +VG++ N 
Sbjct: 34  YLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGATQNL 93

Query: 68  FSFFIIWLSVTHRIAMPQFWQMFLYICLATNAQNFANTAVMVISVENFPDRRGIILGLLK 127
           F + ++WL VT ++     W +F+ I + TN + + NTA +V  + NFP+ RG ++G+LK
Sbjct: 94  FGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVGILK 153

Query: 128 GFVGLGGAVLTQIYLAIYGDKDASDVLLLLAWFPSIMSMVLLFSIRTI----KARKHPEE 187
           GF GL GA+LTQ+YL      D+S V+L++A  P ++ + LLF +R +    +     ++
Sbjct: 154 GFSGLSGAILTQVYLMFNPSHDSS-VILMVALGPPVVVLALLFIVRPVERSCRTNLRSDD 213

Query: 188 LKVFYHLLYASIILAVFIFILTLTQKHIVFSHVGYVGGVVVVIAFLCLPLLI 235
           L+ F  +    ++LAV++  L + Q     +         +++ F+ +P+L+
Sbjct: 214 LR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLV 263

The following BLAST results are available for this feature:

BLAST of MC03g_new0222 vs.


Match Name	E-value	Identity	Description

BLAST of MC03g_new0222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152848.1	3.18e-133	85.29	uncharacterized membrane protein YMR155W-like [Momordica charantia]	[more]
XP_022152849.1	5.60e-120	76.47	uncharacterized protein LOC111020473 [Momordica charantia]	[more]
XP_008464767.1	9.34e-110	68.91	PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo] >TYK17154.1 prote...	[more]
XP_004147309.1	1.28e-109	68.91	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8652749.1 hypothet...	[more]
KAG6583552.1	2.16e-108	68.91	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of MC03g_new0222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DG00	1.54e-133	85.29	uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1DHB0	2.71e-120	76.47	uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A5D3D1P8	4.52e-110	68.91	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CMR5	4.52e-110	68.91	uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=...	[more]
A0A6J1HLW5	1.92e-108	68.91	uncharacterized protein LOC111464774 OS=Cucurbita moschata OX=3662 GN=LOC1114647...	[more]

BLAST of MC03g_new0222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	3.8e-62	47.48	Major facilitator superfamily protein	[more]
AT2G39210.1	6.0e-60	47.06	Major facilitator superfamily protein	[more]
AT3G01930.2	1.0e-35	36.80	Major facilitator superfamily protein	[more]
AT5G14120.1	6.7e-35	35.93	Major facilitator superfamily protein	[more]
AT5G50630.1	8.2e-33	34.48	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 1..189 e-value: 2.2E-5 score: 25.5
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 7..233
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 6..236 e-value: 8.0E-71 score: 238.4
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 1..238
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 1..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0222.1	MC03g_new0222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity