MC03g0812 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0812
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	polyphenol oxidase, chloroplastic-like
Location	MC03: 14792684 .. 14794562 (+)
RNA-Seq Expression	MC03g0812
Synteny	MC03g0812

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCCATTGCCGCCATCACTTCTCCCATCATCCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATGCTCGAGTTGTGTGCAGCGGCTCCAACAAGCATGATTCCCAGTCCCCAGGGAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATATGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGTCACATTTTCCTAACCACATATTTTAAGCTTTTAACACTTTATCCTTTTTAAATTACAATCATATCTCAATTCTGTATCTCTTAGCTAGCGATAAGAGGTGTGAGGTGTTAATCAATCCTAGCCATGAATCTTGATTATAATAAATATTACAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTGAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGCGGCATCGAGATTGAATTTGAC

mRNA sequence

CCTCCATTGCCGCCATCACTTCTCCCATCATCCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATGCTCGAGTTGTGTGCAGCGGCTCCAACAAGCATGATTCCCAGTCCCCAGGGAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATATGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTGAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGCGGCATCGAGATTGAATTTGAC

Coding sequence (CDS)

CCTCCATTGCCGCCATCACTTCTCCCATCATCCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATGCTCGAGTTGTGTGCAGCGGCTCCAACAAGCATGATTCCCAGTCCCCAGGGAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATATGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTGAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGCGGCATCGAGATTGAATTTGAC

Protein sequence

PPLPPSLLPSSRRKFVVNKNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASSFAPEYDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD

Homology

[Close]

BLAST of MC03g0812 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 4.5e-191
Identity = 334/583 (57.29%), Postives = 413/583 (70.84%), Query Frame = 0


Query: 2   PLPPSLLPSSRRKFVVNKNNKHAR-VVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASS 61
           PL P    +S+   +       AR V C  +N   +    + +DRR+VL+GLGGLYG + 
Sbjct: 21  PLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQSKLDRRNVLLGLGGLYGVAG 80

Query: 62  FAPEYDSFAIAAPVDTPQITKCGPAQVRDKKV--ECCPPPSIKIKDFRPTPPRKLRVRPA 121
                D FA A P+  P ++KCGPA +    V   CCPPPS KI DF+   P KLR+RP 
Sbjct: 81  MGT--DPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPP 140

Query: 122 AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFP-VELQVHNS 181
           AH VD+ Y  KY +A+ELM+ LPD DPRSF QQA +HCAYC+G Y Q+GFP +ELQ+HNS
Sbjct: 141 AHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNS 200

Query: 182 WLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNE 241
           WLFFPFHR+YLYF EKILG+LI DPTFALPFWN+D+PAGM  PAI+A+  SPLYD+ R+ 
Sbjct: 201 WLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSA 260

Query: 242 NHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPG 301
           NH PPT+VDLDYNGT+ N+  E  + +NL++MYRQMVS S+   LFFG PYRAGD+P PG
Sbjct: 261 NHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPG 320

Query: 302 AGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRR 361
            GS+E  PH P+H W G+  QPN EDMGNFYSAGRDP+F++HH+NVDRMW IWKTLG +R
Sbjct: 321 GGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKR 380

Query: 362 RDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLR 421
            D+ + D+L++ F+FYNE  E VRV+VRDCL+T  LGYVYQDVDIPWL + P+PRR K+ 
Sbjct: 381 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 440

Query: 422 KGGVANNNMPKFPSLLGTG-AALAAESEL----EFPLSLESVIRTDVKRPK-KSRSSKEK 481
              VA          LG   AA+A+ S++    EFP+SL S I T VKRPK K RS K K
Sbjct: 441 LSKVAKK--------LGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAK 500

Query: 482 EEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKT 541
           E+EEEILVI GIEFD + AVKFDV +N  +D     D TEFAGSF SVPH + +   + T
Sbjct: 501 EDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFVSVPHSHKHKKKMNT 560

Query: 542 CLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 575
            LRLG+T+LLE++  ED DSVVVTLVP  K   + IGGI+IEF
Sbjct: 561 ILRLGLTDLLEEIEAEDDDSVVVTLVP--KFGAVKIGGIKIEF 591

[Close]

BLAST of MC03g0812 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.9e-169
Identity = 305/587 (51.96%), Postives = 394/587 (67.12%), Query Frame = 0


Query: 6   SLLPSSRRKFVVNKNNKHAR-VVCSGSNKH----DSQSPGNMV-DRRDVLIGLGGLYGAS 65
           S+ P  +++   +K  K  R V CS +N      + Q   N+V  RR+VLIGLGG+YG  
Sbjct: 25  SVYPFLQKQHQSSKLRKPKRQVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIYGTL 84

Query: 66  SFAPEYDSFAIAAPVDTPQITKCG-----PAQVRDKKVECCPPPSIKIKDFRPTPPRKLR 125
           +  P     A+A+P+  P ++KC      P+      + CCPP S KI DF+    + LR
Sbjct: 85  ATNPS----ALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQPLR 144

Query: 126 VRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFP-VELQ 185
           VR AAHLVD  +L+KY++A ELM+ LP +DPR+F QQANIHCAYC+G Y Q+GFP ++LQ
Sbjct: 145 VRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQ 204

Query: 186 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 245
           VH SWLFFPFHR+YLYF+E+ILG LI DPTFALPFWNYDAP GM  P I+A++ SPLYDE
Sbjct: 205 VHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDE 264

Query: 246 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 305
            RN +H PPT++DL++     ++D    +++NL +MYRQ+ S  +T  LF G PYRAGD 
Sbjct: 265 LRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAGDA 324

Query: 306 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 365
              GAGS+ENVPH P+H W G+  Q N EDMG FYSA RDP+FYSHH+NVDR+W IWKTL
Sbjct: 325 EPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTL 384

Query: 366 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 425
           G ++ D  + D+L + F+FY+E K  VRV V+D LD   LGY YQDV IPW K  P PRR
Sbjct: 385 GGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRR 444

Query: 426 KKLRK---GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKE 485
            K++K     V + N+ K P++       +    + FPL L + +   VKRPKK RS KE
Sbjct: 445 TKVQKLVEVEVNDGNLRKSPTIF-LVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKE 504

Query: 486 KEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLK 545
           KEEEEE+LVI GIEF  N A+KFDV IN E+D+ + A NTEFAGSF ++PH      N K
Sbjct: 505 KEEEEEVLVIEGIEFYMNIAIKFDVYINDEDDK-VGAGNTEFAGSFVNIPHSAHGHKNKK 564

Query: 546 --TCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 576
             T LRLGIT+LLEDL +E  D++VVTLVP+     + I  +EI F+
Sbjct: 565 IITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEIVFE 605

[Close]

BLAST of MC03g0812 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.5e-165
Identity = 304/592 (51.35%), Postives = 390/592 (65.88%), Query Frame = 0


Query: 4   PPSLLPSSRRKFVVNKNNKH----ARVVCSGSN---KHDS-----QSPGNMVDRRDVLIG 63
           PPS L        V +N       ++V C+ +N     DS     ++    +DRR+VL+G
Sbjct: 24  PPSPLFQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLG 83

Query: 64  LGGLYGASSFAPEYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFR-PT 123
           +GGLYGA+         A  AP+  P I+KCG A V D      CCPP + KI DF+ P+
Sbjct: 84  IGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPS 143

Query: 124 PPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGF 183
               +R RPAAHLV K YL KY++A+EL + LPD DPRSF QQAN+HC YC G Y Q+G+
Sbjct: 144 SGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGY 203

Query: 184 -PVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQN 243
             +ELQVH SWLF PFHR+YLYF+E+IL +LI DPTFALP+W +D P GM  P I+A+  
Sbjct: 204 TDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSP 263

Query: 244 SPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRP 303
           S LYDE RN  H+PPTV+DLDY+GT+  + D+     NL +MY+Q+VSG+ T  LF G P
Sbjct: 264 SSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYP 323

Query: 304 YRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMW 363
           YRAGD   PGAG++E+ PH  +H+W G   +P+ EDMGNFY+AGRDP+F+ HHANVDRMW
Sbjct: 324 YRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDPIFFGHHANVDRMW 383

Query: 364 CIWKTL-GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLK 423
            IWKT+ G+ R+D  + D+L+A+FVFY+E K+ V+V+V DC+DT+ L Y YQD+ IPWL 
Sbjct: 384 NIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLP 443

Query: 424 TAPSPRRK---KLRKGGVAN-NNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRP 483
                + K   K  K GVA    +PK              S  +FP +L SVIR +V RP
Sbjct: 444 KNTKAKAKTTTKSSKSGVAKAAELPK----------TTISSIGDFPKALNSVIRVEVPRP 503

Query: 484 KKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQ 543
           KKSRS KEKE+EEE+L+I GIE D    VKFDV IN E+    R  N+EFAGSF +VPH+
Sbjct: 504 KKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNVPHK 563

Query: 544 NANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 575
           +      KT LR  I ELLEDLG ED +SV+VT+VPR    ++TIGGIEIEF
Sbjct: 564 HMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEF 604

[Close]

BLAST of MC03g0812 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 284/568 (50.00%), Postives = 381/568 (67.08%), Query Frame = 0


Query: 19  KNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASSFAPEYDSFAIAAPVDTPQ 78
           K   H +V C+  N  DSQ     +DRRDVL+GLGGL GA+S     +  A A P+  P+
Sbjct: 42  KRTHHFKVSCNAPN-GDSQP---KLDRRDVLLGLGGLAGAASLI--NNPLAFAEPIHAPE 101

Query: 79  ITKC--GPAQVRDKKV--ECCPPPSIKIKDFR--PTPPRKLRVRPAAHLVDKNYLKKYER 138
           I+KC   P  +    +   CCPP +  +  ++   T P  +++RPA H +DK Y+ K+E+
Sbjct: 102 ISKCVVPPKDLPPDAIVDNCCPPLATNVIPYKVPKTSPSAMKIRPAIHRMDKEYIAKFEK 161

Query: 139 AVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFP-VELQVHNSWLFFPFHRFYLYFH 198
           A+ LM+ LP  DPR+F QQA +HCAYCNGGY Q  +P  E+QVHNSWLFFPFHR+YLYF+
Sbjct: 162 AIRLMKELPADDPRNFYQQALVHCAYCNGGYVQTDYPDKEIQVHNSWLFFPFHRWYLYFY 221

Query: 199 EKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNG 258
           E+ILG+LIGDPTF LPFWN+D PAGM  P  F NQNSPLYDE RN++H+ P V+DL Y G
Sbjct: 222 ERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRNQNSPLYDENRNQSHL-PLVMDLGYAG 281

Query: 259 TDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDP-SPGAGSVENVPHIPIH 318
           TD ++ D+ R+ +NL +MY+ MV+ + T  LF G+PY+AGDDP + G GS+EN+PH P+H
Sbjct: 282 TDTDVTDQERISNNLALMYKSMVTNAGTAELFLGKPYKAGDDPVNKGGGSIENIPHTPVH 341

Query: 319 RWCGN--KHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLG--RRRRDIQNPDFL 378
           RW G+      NGEDMGNFYSAGRD +FY HH+NVDRMW IW+ LG   RRRD  + D+L
Sbjct: 342 RWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNVDRMWTIWQQLGGKGRRRDFTDSDWL 401

Query: 379 NASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNM 438
           +A+F+FY+E K+AVRVRV D LD   LGY Y+  ++PWL + P P +KK    G+A  + 
Sbjct: 402 DATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTNLPWLNSKPLPTKKKT---GLAARSK 461

Query: 439 PKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFD 498
             F + +             FPL+L+ V++  V RPKKSRS +EKE EEEIL I GIE  
Sbjct: 462 APFVTDV-------------FPLTLDKVVQVKVPRPKKSRSKEEKEAEEEILEIQGIEVA 521

Query: 499 PNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGI 558
            +   KFDV +N E++ E   +  E+AGSFA +PH++     ++T L LG+ E LEDLG 
Sbjct: 522 IDQYAKFDVYLNDEDEPEAGKEKAEYAGSFAHLPHKHTGSKKIRTSLSLGLNEPLEDLGA 581

Query: 559 EDQDSVVVTLVPRIKDQNITIGGIEIEF 575
           ED D+V+VTL P++    +T+  I+I +
Sbjct: 582 EDDDAVLVTLAPKVGGGVVTVENIKIVY 586

[Close]

BLAST of MC03g0812 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 5.6e-157
Identity = 283/570 (49.65%), Postives = 373/570 (65.44%), Query Frame = 0


Query: 22  KHARVVCSGSNKHDSQSPGNM----------VDRRDVLIGLGGLYGASSFAPEYDSFAIA 81
           + + + C  +N ++S +              +DRR++LIGLGGLYGA++   +   FA A
Sbjct: 44  RRSSISCKATNNNNSNNQNEQQEESSRLLGKLDRRNILIGLGGLYGATTL--DRKPFAFA 103

Query: 82  APVDTPQITKCGPAQVR---DKKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLK 141
            P+  P +T C PA++     + V CCPP + KIK F+P     LR  PAAH V   YL 
Sbjct: 104 DPIAPPDLTTCKPAEITPGGSETVPCCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLA 163

Query: 142 KYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGF-PVELQVHNSWLFFPFHRFY 201
           K+++A   MR LPD DPRS +QQA +HCAYCNG Y Q+GF   ++QVH SWLFFPFHR Y
Sbjct: 164 KFKKAQAAMRALPDDDPRSMVQQAKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMY 223

Query: 202 LYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDL 261
           LYF+E+ILG+LI DPTFALP+WN+D+P G   P I+ + +SPLYD++RN +H PP +VDL
Sbjct: 224 LYFYERILGKLIDDPTFALPYWNWDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDL 283

Query: 262 DYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD-DPSPGAGSVENVPH 321
            Y G D ++D++ R+  NL +MYRQMVSG++T  LFFG  YRAG+ +     G++EN+PH
Sbjct: 284 SYGGKDDDVDEQTRIDENLAIMYRQMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPH 343

Query: 322 IPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFL 381
             IH W G+  Q + EDMGNFYSAGRDP+FY+HHANVDRMW IWKTLG +R+DI + D+L
Sbjct: 344 NNIHIWVGDPSQTHQEDMGNFYSAGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWL 403

Query: 382 NASFVFYNEKKEAVRVRVRDCLD-TTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNN 441
           +A F+FY+E  E VRV+VRD L+    L Y Y+ V +PWL T P+ R+ K       N  
Sbjct: 404 DAEFLFYDENAELVRVKVRDSLEPEKQLRYNYEPVSLPWLFTKPTARKTK-------NKT 463

Query: 442 MPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPK-KSRSSKEKEEEEEILVISGIE 501
             K           A +   +FP +L  V   +V RPK + RS KEK +EEE+L+I  IE
Sbjct: 464 KAK---------VAATQLTSKFPATLVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIE 523

Query: 502 FDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDL 561
           F+   AVKFDV IN + +   R D +EFAGSF  VP         KT L+LGIT+LLEDL
Sbjct: 524 FEGTEAVKFDVFINDDAESLSRRDKSEFAGSFVHVPQGKTTKAKTKTNLKLGITDLLEDL 583

Query: 562 GIEDQDSVVVTLVPRIKDQNITIGGIEIEF 575
           G ED  SV+VTLVPR+ +  ITIGG +IE+
Sbjct: 584 GAEDDSSVLVTLVPRVSNSPITIGGFKIEY 595

[Close]

BLAST of MC03g0812 vs. NCBI nr
Match: XP_022139808.1 (polyphenol oxidase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1171 bits (3030), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 571/577 (98.96%), Query Frame = 0


Query: 1   PPLPPSLLPSSRRKFVVNKNNKHARVVCSGSNKHDSQS--PGNMVDRRDVLIGLGGLYGA 60
           PPLPPSLLPS RRKFVVNKNNKH RVVCS SNKHDSQS  PGNMVDRRDVLIGLGGLYGA
Sbjct: 14  PPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGA 73

Query: 61  SSFAPEYDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA 120
           SSFAPE DSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA
Sbjct: 74  SSFAPEXDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA 133

Query: 121 AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW 180
           AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW
Sbjct: 134 AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW 193

Query: 181 LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN 240
           LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN
Sbjct: 194 LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN 253

Query: 241 HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA 300
           HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA
Sbjct: 254 HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA 313

Query: 301 GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR 360
           GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR
Sbjct: 314 GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR 373

Query: 361 DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK 420
           DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK
Sbjct: 374 DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK 433

Query: 421 GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL 480
           GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL
Sbjct: 434 GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL 493

Query: 481 VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT 540
           VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT
Sbjct: 494 VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT 553

Query: 541 ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 575
           ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD
Sbjct: 554 ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 590

[Close]

BLAST of MC03g0812 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 738 bits (1904), Expect = 9.15e-262
Identity = 357/582 (61.34%), Postives = 446/582 (76.63%), Query Frame = 0


Query: 3   LPPSLL------PSSRRKFVVNKNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLG--G 62
           LPP  L      PSSR        N  ARV+CS +N          +DRRDVLIG+G  G
Sbjct: 4   LPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAAN--------GKIDRRDVLIGIGAGG 63

Query: 63  LYGASSFAPEYDSFAIAAPVDTPQITKCGPAQVR--DKKVECCPPPSIKIKDFRPTPPRK 122
           +YGAS+F  +  +FA+AAPV TP I+KCGP  +        CCPPPS  I DFRP  P K
Sbjct: 64  IYGASAFTKD-STFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGK 123

Query: 123 LRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVEL 182
           LRVRPAAHL D++Y+ KY+RAVELM+GLP  DPRSFMQQAN+HCAYCNGGY Q+GFPVE+
Sbjct: 124 LRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEV 183

Query: 183 QVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYD 242
           QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+ NS LYD
Sbjct: 184 QVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYD 243

Query: 243 EFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD 302
           + RN+NH+PPT++DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  Y AGD
Sbjct: 244 QLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGD 303

Query: 303 DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKT 362
           +PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+NVDRMW +WKT
Sbjct: 304 EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKT 363

Query: 363 LGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPR 422
           LG RRRD+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++PWL+T P+PR
Sbjct: 364 LGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPR 423

Query: 423 RKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKE 482
           R  LR     ++N+P         +      +++ P++L+S I T+VKR KKSR  KEK+
Sbjct: 424 RA-LRAQKSVDSNVP---------SRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKD 483

Query: 483 EEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTC 542
           EEEEILVI GIEFDPNT++K D+ IN E+DREIR DNTEFAGSF +VPH++ +   +KT 
Sbjct: 484 EEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTG 543

Query: 543 LRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 574
           LRLGI+E+LEDL  +D DS++VT++PR+    ++I  I+IE+
Sbjct: 544 LRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 566

[Close]

BLAST of MC03g0812 vs. NCBI nr
Match: XP_038903169.1 (polyphenol oxidase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 738 bits (1904), Expect = 1.95e-261
Identity = 358/587 (60.99%), Postives = 443/587 (75.47%), Query Frame = 0


Query: 4   PPSL---LPSSRRKFVVNK---NNKHARVV-CSGSNKHDSQSPGNMVDRRDVLIGLGGLY 63
           PPSL    PSS       K   NN   R++ C+  N +D  +P   +DRRD+L+GL GLY
Sbjct: 7   PPSLTTYFPSSNSFLPKTKTLPNNISPRIISCNTVNHNDQNTPKPKIDRRDILVGLSGLY 66

Query: 64  GASSFAPEYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLR 123
           GAS+      +FA+AAP+ TP I+KCGPA +        CCPPP+ +I DF+P    KLR
Sbjct: 67  GASTLYKN-SNFALAAPIQTPNISKCGPADLPPGADSTNCCPPPATRILDFQPPAQEKLR 126

Query: 124 VRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQV 183
           VRPAAHLVDKNY++KY++AVELM+ LP++DPRSF QQAN+HCAYCNG Y Q+GFPVE+QV
Sbjct: 127 VRPAAHLVDKNYIEKYKKAVELMKALPENDPRSFTQQANVHCAYCNGAYDQVGFPVEVQV 186

Query: 184 HNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEF 243
           HNSWLFFPFHR YLYFHEKILGELIGDP FALPFWNYDAP GM  P ++A + S LYDE 
Sbjct: 187 HNSWLFFPFHRLYLYFHEKILGELIGDPNFALPFWNYDAPGGMRMPEMYAEEKSSLYDEL 246

Query: 244 RNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDP 303
           RNENH+PP +VDLD+   D N+ D+A++RSNL +MYRQMVS SRT SLFFG  YRAGD+P
Sbjct: 247 RNENHVPPRLVDLDFGRVDPNIGDDAQIRSNLSIMYRQMVSNSRTPSLFFGSAYRAGDEP 306

Query: 304 SPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLG 363
           SPG GS+EN+PH P+H WCG+ ++PNGE+MGNFYSA RDP+FY+HH+N+DR+W +WKTLG
Sbjct: 307 SPGGGSIENIPHGPVHVWCGDNNEPNGENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLG 366

Query: 364 RRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRK 423
            RR+D+QNP++LN SF+FYNEK EAVRVRVR  LD+  LGYVYQDVD+PWL+T P+PRR 
Sbjct: 367 GRRQDLQNPEWLNTSFIFYNEKAEAVRVRVRQSLDSKILGYVYQDVDVPWLQTRPTPRRA 426

Query: 424 KLRKGGVANNNMPKFPSLLGTGAALAAESE------LEFPLSLESVIRTDVKRPKKSRSS 483
           +     VANNN+   P  +G   A    S       ++FP++++S +  +VKRPKKSRS 
Sbjct: 427 QKT---VANNNII-LPFSIGPALAAGPSSSRVRPANIKFPITVKSAVSVEVKRPKKSRSK 486

Query: 484 KEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNN 543
           KEKEEEEEILVI GI FDPN  +KFDV IN E+D+EIR DNTEFAGSF +VPH+  +   
Sbjct: 487 KEKEEEEEILVIYGIGFDPNQGIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKLGHKKT 546

Query: 544 LKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 575
           +KT LRLGITE+LEDL  +D DSV VT+VPR+     +I GI+I  D
Sbjct: 547 VKTGLRLGITEILEDLEADDDDSVRVTIVPRLGAGLFSIDGIKIVCD 588

[Close]

BLAST of MC03g0812 vs. NCBI nr
Match: APO14262.1 (polyphenol oxidase 3 [Luffa aegyptiaca])

HSP 1 Score: 736 bits (1900), Expect = 9.15e-261
Identity = 356/578 (61.59%), Postives = 444/578 (76.82%), Query Frame = 0


Query: 4   PPSLLPSSRRKFVVNKNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASSFAP 63
           PP+L    R K+    ++K  R++CS SN        N +DRRD+LIGLGGLYGAS+ + 
Sbjct: 28  PPNL---RRTKYF---SSKAPRILCSASNGGQ-----NHIDRRDILIGLGGLYGASTLSQ 87

Query: 64  EYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHL 123
              S A AAPV TP I+KCG   +      + CCPPP+  + DFRP  P KLRVRPAAHL
Sbjct: 88  --GSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQAPGKLRVRPAAHL 147

Query: 124 VDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFF 183
            D+NY++KY+RAVELM+ LPD DPRSF QQAN+HCAYCNG Y Q+GFPVE+QVHNSWLFF
Sbjct: 148 ADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFF 207

Query: 184 PFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMP 243
           PFHR+YL+FHEKILGELIGDP+FALPFWNYDAP GM  PA++A++ S LYDE RN NH+P
Sbjct: 208 PFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKSSLYDELRNRNHLP 267

Query: 244 PTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSV 303
           PT+VDLD+ G D  + DEA++RSNL +MYRQMVS SRT SLFFG  Y AGD+PSPG G V
Sbjct: 268 PTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAYLAGDEPSPGGGLV 327

Query: 304 ENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQ 363
           EN+PH P+H WCG+ ++PN E+MGNFYSA RDP+FY+HH+N+DR+W +WKTLG RR+D+Q
Sbjct: 328 ENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQ 387

Query: 364 NPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGV 423
           N D+LNASF+FY+EK +AVRVRVRDCLD+  LGYVYQDV +PWL++ P+PRR    +  V
Sbjct: 388 NADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESRPTPRRALRAQKAV 447

Query: 424 ANNNMPKFPSLL----GTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEI 483
           A+      P+L     G  ++    ++++FP++L S I  +VKRPK+SRS KEK+EEEEI
Sbjct: 448 AHLPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRPKRSRSKKEKDEEEEI 507

Query: 484 LVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGI 543
           +VI GIEFD N A+KFDV IN E+D+EIR DNTEFAGSF +VPH++ +  N+KT LRLGI
Sbjct: 508 IVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHGHKKNVKTGLRLGI 567

Query: 544 TELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 575
           TELLEDL  +D DSV VTLVPR     ++I GI+IEFD
Sbjct: 568 TELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFD 592

[Close]

BLAST of MC03g0812 vs. NCBI nr
Match: XP_023519283.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731 bits (1886), Expect = 4.97e-259
Identity = 349/580 (60.17%), Postives = 448/580 (77.24%), Query Frame = 0


Query: 4   PPSLLPSSRRKFVVNKNNKH-----ARVVCSGSNKHDSQSPGNMVDRRDVLIGLG--GLY 63
           PP+L  ++ R     + +K+     AR++CS ++          +DRRDVLIG+G  GLY
Sbjct: 6   PPALSTAAFRPSSSLRTSKYLPINAARILCSAAD--------GKIDRRDVLIGIGAGGLY 65

Query: 64  GASSFAPEYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLR 123
           GAS+F  +  +FA+AAPV TP I+KCGP  +        CCPPP+  I DF P  P KLR
Sbjct: 66  GASAFTKD-STFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIVDFLPPAPGKLR 125

Query: 124 VRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQV 183
           VRPAAHL D++Y+ KY+RAVELM+GLP+ DPRSFMQQAN+HCAYCNGGY Q+GFPVE+QV
Sbjct: 126 VRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQV 185

Query: 184 HNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEF 243
           HNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+QNS LYD+ 
Sbjct: 186 HNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQL 245

Query: 244 RNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDP 303
           RN+NH+PPT++DLD++G D N+ + A++R+NLR+MYRQMVS SRT  LFFG  Y AGD+P
Sbjct: 246 RNQNHLPPTLIDLDFDGVDSNIGNNAQIRTNLRIMYRQMVSNSRTPKLFFGSAYVAGDEP 305

Query: 304 SPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLG 363
           SPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+N+DRMW +WKTLG
Sbjct: 306 SPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLG 365

Query: 364 RRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRK 423
            RR+D+Q P++L+ASF+FYNEK EAVRVRVRDCLDT  LGY+YQDV++PWL+T P+PRR 
Sbjct: 366 GRRQDLQYPEWLDASFIFYNEKGEAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRA 425

Query: 424 KLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEE 483
            LR     ++N+P         +       ++FP+++ES + T+VKR KKSRS KEK+EE
Sbjct: 426 -LRAQKSVDSNVP---------SRRVKPGRMKFPVTVESAVSTEVKRGKKSRSRKEKDEE 485

Query: 484 EEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLR 543
           EEILVI GIEFDPNTA+K DV IN E++REIR DNTEFAGSF +VPH++ +   +KT LR
Sbjct: 486 EEILVIKGIEFDPNTAIKLDVYINDEDEREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLR 545

Query: 544 LGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 574
           LGITE+LEDL  +D DS++VT++P +    ++I  I +E+
Sbjct: 546 LGITEVLEDLEADDDDSIIVTIIPSLGAGLVSIADITLEY 566

[Close]

BLAST of MC03g0812 vs. ExPASy TrEMBL
Match: A0A6J1CGK8 (polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010634 PE=3 SV=1)

HSP 1 Score: 1171 bits (3030), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 571/577 (98.96%), Query Frame = 0


Query: 1   PPLPPSLLPSSRRKFVVNKNNKHARVVCSGSNKHDSQS--PGNMVDRRDVLIGLGGLYGA 60
           PPLPPSLLPS RRKFVVNKNNKH RVVCS SNKHDSQS  PGNMVDRRDVLIGLGGLYGA
Sbjct: 14  PPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGA 73

Query: 61  SSFAPEYDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA 120
           SSFAPE DSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA
Sbjct: 74  SSFAPEXDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPA 133

Query: 121 AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW 180
           AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW
Sbjct: 134 AHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSW 193

Query: 181 LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN 240
           LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN
Sbjct: 194 LFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNEN 253

Query: 241 HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA 300
           HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA
Sbjct: 254 HMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGA 313

Query: 301 GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR 360
           GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR
Sbjct: 314 GSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRR 373

Query: 361 DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK 420
           DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK
Sbjct: 374 DIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRK 433

Query: 421 GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL 480
           GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL
Sbjct: 434 GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEIL 493

Query: 481 VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT 540
           VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT
Sbjct: 494 VISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGIT 553

Query: 541 ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 575
           ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD
Sbjct: 554 ELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 590

[Close]

BLAST of MC03g0812 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 738 bits (1904), Expect = 4.43e-262
Identity = 357/582 (61.34%), Postives = 446/582 (76.63%), Query Frame = 0


Query: 3   LPPSLL------PSSRRKFVVNKNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLG--G 62
           LPP  L      PSSR        N  ARV+CS +N          +DRRDVLIG+G  G
Sbjct: 4   LPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAAN--------GKIDRRDVLIGIGAGG 63

Query: 63  LYGASSFAPEYDSFAIAAPVDTPQITKCGPAQVR--DKKVECCPPPSIKIKDFRPTPPRK 122
           +YGAS+F  +  +FA+AAPV TP I+KCGP  +        CCPPPS  I DFRP  P K
Sbjct: 64  IYGASAFTKD-STFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGK 123

Query: 123 LRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVEL 182
           LRVRPAAHL D++Y+ KY+RAVELM+GLP  DPRSFMQQAN+HCAYCNGGY Q+GFPVE+
Sbjct: 124 LRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEV 183

Query: 183 QVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYD 242
           QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+ NS LYD
Sbjct: 184 QVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYD 243

Query: 243 EFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD 302
           + RN+NH+PPT++DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  Y AGD
Sbjct: 244 QLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGD 303

Query: 303 DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKT 362
           +PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+NVDRMW +WKT
Sbjct: 304 EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKT 363

Query: 363 LGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPR 422
           LG RRRD+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++PWL+T P+PR
Sbjct: 364 LGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPR 423

Query: 423 RKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKE 482
           R  LR     ++N+P         +      +++ P++L+S I T+VKR KKSR  KEK+
Sbjct: 424 RA-LRAQKSVDSNVP---------SRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKD 483

Query: 483 EEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTC 542
           EEEEILVI GIEFDPNT++K D+ IN E+DREIR DNTEFAGSF +VPH++ +   +KT 
Sbjct: 484 EEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTG 543

Query: 543 LRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 574
           LRLGI+E+LEDL  +D DS++VT++PR+    ++I  I+IE+
Sbjct: 544 LRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 566

[Close]

BLAST of MC03g0812 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 736 bits (1900), Expect = 4.43e-261
Identity = 356/578 (61.59%), Postives = 444/578 (76.82%), Query Frame = 0


Query: 4   PPSLLPSSRRKFVVNKNNKHARVVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASSFAP 63
           PP+L    R K+    ++K  R++CS SN        N +DRRD+LIGLGGLYGAS+ + 
Sbjct: 28  PPNL---RRTKYF---SSKAPRILCSASNGGQ-----NHIDRRDILIGLGGLYGASTLSQ 87

Query: 64  EYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHL 123
              S A AAPV TP I+KCG   +      + CCPPP+  + DFRP  P KLRVRPAAHL
Sbjct: 88  --GSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQAPGKLRVRPAAHL 147

Query: 124 VDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFF 183
            D+NY++KY+RAVELM+ LPD DPRSF QQAN+HCAYCNG Y Q+GFPVE+QVHNSWLFF
Sbjct: 148 ADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFF 207

Query: 184 PFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMP 243
           PFHR+YL+FHEKILGELIGDP+FALPFWNYDAP GM  PA++A++ S LYDE RN NH+P
Sbjct: 208 PFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKSSLYDELRNRNHLP 267

Query: 244 PTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSV 303
           PT+VDLD+ G D  + DEA++RSNL +MYRQMVS SRT SLFFG  Y AGD+PSPG G V
Sbjct: 268 PTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAYLAGDEPSPGGGLV 327

Query: 304 ENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQ 363
           EN+PH P+H WCG+ ++PN E+MGNFYSA RDP+FY+HH+N+DR+W +WKTLG RR+D+Q
Sbjct: 328 ENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQ 387

Query: 364 NPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGV 423
           N D+LNASF+FY+EK +AVRVRVRDCLD+  LGYVYQDV +PWL++ P+PRR    +  V
Sbjct: 388 NADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESRPTPRRALRAQKAV 447

Query: 424 ANNNMPKFPSLL----GTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEI 483
           A+      P+L     G  ++    ++++FP++L S I  +VKRPK+SRS KEK+EEEEI
Sbjct: 448 AHLPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRPKRSRSKKEKDEEEEI 507

Query: 484 LVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGI 543
           +VI GIEFD N A+KFDV IN E+D+EIR DNTEFAGSF +VPH++ +  N+KT LRLGI
Sbjct: 508 IVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHGHKKNVKTGLRLGI 567

Query: 544 TELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 575
           TELLEDL  +D DSV VTLVPR     ++I GI+IEFD
Sbjct: 568 TELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEFD 592

[Close]

BLAST of MC03g0812 vs. ExPASy TrEMBL
Match: A0A0A0LXD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1)

HSP 1 Score: 729 bits (1883), Expect = 9.51e-259
Identity = 335/553 (60.58%), Postives = 435/553 (78.66%), Query Frame = 0


Query: 25  RVVCSGSNKHDSQSPGNMVDRRDVLIGLGGLYGASSFAPEYDSFAIAAPVDTPQITKCGP 84
           R+ C  +N +D   P   +DRRD+LIGL GLYG+S+ + +   FA+AAP++TP I+KCGP
Sbjct: 27  RISCKATNHNDQNFPNPKIDRRDILIGLTGLYGSSTLS-QSTHFALAAPIETPNISKCGP 86

Query: 85  AQVRD--KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPD 144
             +        CCPPP+  I DF+P PP KLRVRPAAHL D+NY++KY++AVELM+ LP+
Sbjct: 87  PDLPPGADATNCCPPPATTILDFQPPPPSKLRVRPAAHLADRNYIEKYKKAVELMKALPE 146

Query: 145 HDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDP 204
            DPRSF QQAN+HCAYCNG Y Q+GFPVE+QVHNSWLFFPFHRFYLYFHEKILGELIGDP
Sbjct: 147 DDPRSFTQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDP 206

Query: 205 TFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARV 264
           +FALPFWNYDAP GM  P ++A + S LYDE RNENH+PP +VDLD+ G D N+ D+A++
Sbjct: 207 SFALPFWNYDAPDGMKMPGMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDPNIGDDAQI 266

Query: 265 RSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGE 324
           +SNL +MYRQMVSGSRT SLFFG  Y AGD+PSPG GSVEN+PH P+H WCG+  QPN E
Sbjct: 267 KSNLSIMYRQMVSGSRTPSLFFGSAYVAGDEPSPGGGSVENIPHGPVHVWCGDTDQPNFE 326

Query: 325 DMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRV 384
           +MGNFYSA RDP+F++HH+N+DR+W +WKTLG RR+++QNP++LNASF+FY+EK + +RV
Sbjct: 327 NMGNFYSAARDPIFFAHHSNIDRLWSVWKTLGGRRQNLQNPEWLNASFIFYDEKAQPIRV 386

Query: 385 RVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAE 444
           RVRDCLD+ +LGY+YQ VD+PWL+T P+PRR + +   VA    P   +  G  ++    
Sbjct: 387 RVRDCLDSKSLGYIYQPVDLPWLQTRPTPRRAQ-KTSTVAFGIAPALAAG-GPSSSRVKP 446

Query: 445 SELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEED 504
           + ++FP++++S +  +VKRPKKSRS KEKEEEEE+LVI GI FDPN  +KFDV IN E+D
Sbjct: 447 ANIKFPITVKSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDD 506

Query: 505 REIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKD 564
           +EI+ DNTEFAGSF +VPH++ +   +KT LR+GITE LEDL  ++ DS++VT++PRI  
Sbjct: 507 KEIKPDNTEFAGSFVNVPHKHGHKKKVKTGLRVGITEELEDLEADNDDSIIVTILPRIGA 566

Query: 565 QNITIGGIEIEFD 575
              +I G++IEFD
Sbjct: 567 GLFSIDGLKIEFD 576

[Close]

BLAST of MC03g0812 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 729 bits (1882), Expect = 9.75e-259
Identity = 350/580 (60.34%), Postives = 447/580 (77.07%), Query Frame = 0


Query: 4   PPSLLPSSRRKFVVNKNNKH-----ARVVCSGSNKHDSQSPGNMVDRRDVLIGLG--GLY 63
           PP+L  ++ R     + +K+     AR++CS ++          +DRRDVLIG+G  GLY
Sbjct: 6   PPALSTAAFRPSSSLRTSKYLPINAARILCSAAD--------GKIDRRDVLIGIGAGGLY 65

Query: 64  GASSFAPEYDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLR 123
           GASSF  +  +FA+AAPV TP I+KCGP  +        CCPPP+  I DF P  P KLR
Sbjct: 66  GASSFTKD-STFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLR 125

Query: 124 VRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQV 183
           VRPAAHL D++Y+ KY+RAVELM+GLP+ DPRSFMQQAN+HCAYCNGGY Q+GFPVE+QV
Sbjct: 126 VRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQV 185

Query: 184 HNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEF 243
           HNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+QNS LYD+ 
Sbjct: 186 HNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQL 245

Query: 244 RNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDP 303
           RN+NH+PPT +DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  Y AGD+P
Sbjct: 246 RNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEP 305

Query: 304 SPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLG 363
           SPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+N+DRMW +WKTLG
Sbjct: 306 SPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLG 365

Query: 364 RRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRK 423
            RR+D+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++ WL+T P+PRR 
Sbjct: 366 GRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRA 425

Query: 424 KLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEE 483
            LR     ++N+P         +     + ++FP++++S + T+VKR KKSRS KEKEEE
Sbjct: 426 -LRAQKSVDSNVP---------SRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEE 485

Query: 484 EEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLR 543
           EEILVI GIEFDPNTA+K DV IN E+D EIR DNTEFAGSF +VPH++ +   +KT LR
Sbjct: 486 EEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLR 545

Query: 544 LGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 574
           LGI+E+LEDL  +D DS++VT++PR+    ++I  I +E+
Sbjct: 546 LGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 566

The following BLAST results are available for this feature:

BLAST of MC03g0812 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43309	4.5e-191	57.29	Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1	[more]
Q06215	4.9e-169	51.96	Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1	[more]
P43311	1.5e-165	51.35	Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1	[more]
Q9MB14	1.1e-157	50.00	Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=...	[more]
O81103	5.6e-157	49.65	Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP...	[more]

BLAST of MC03g0812 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139808.1	0.0	98.96	polyphenol oxidase, chloroplastic-like [Momordica charantia]	[more]
XP_023001713.1	9.15e-262	61.34	polyphenol oxidase, chloroplastic-like [Cucurbita maxima]	[more]
XP_038903169.1	1.95e-261	60.99	polyphenol oxidase, chloroplastic-like [Benincasa hispida]	[more]
APO14262.1	9.15e-261	61.59	polyphenol oxidase 3 [Luffa aegyptiaca]	[more]
XP_023519283.1	4.97e-259	60.17	polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g0812 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGK8	0.0	98.96	polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1KRC0	4.43e-262	61.34	polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957...	[more]
A0A1L5JHU3	4.43e-261	61.59	Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A0A0LXD0	9.51e-259	60.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1	[more]
A0A6J1EGP5	9.75e-259	60.34	polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of MC03g0812 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002227	Tyrosinase copper-binding domain	PRINTS	PR00092	TYROSINASE	coord: 175..192 score: 56.35 coord: 206..211 score: 55.1 coord: 305..316 score: 32.99 coord: 332..350 score: 56.18
IPR002227	Tyrosinase copper-binding domain	PFAM	PF00264	Tyrosinase	coord: 145..352 e-value: 7.4E-33 score: 114.7
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00498	TYROSINASE_2	coord: 333..344
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00497	TYROSINASE_1	coord: 175..192
IPR022739	Polyphenol oxidase, central domain	PFAM	PF12142	PPO1_DWL	coord: 359..409 e-value: 1.9E-22 score: 78.8
IPR022740	Polyphenol oxidase, C-terminal	PFAM	PF12143	PPO1_KFDV	coord: 447..574 e-value: 9.6E-50 score: 168.0
IPR008922	Uncharacterised domain, di-copper centre	GENE3D	1.10.1280.10		coord: 72..406 e-value: 3.5E-119 score: 400.2
IPR008922	Uncharacterised domain, di-copper centre	SUPERFAMILY	48056	Di-copper centre-containing domain	coord: 88..405
IPR016213	Polyphenol oxidase	PIRSF	PIRSF000290	PPO_plant	coord: 4..575 e-value: 2.7E-249 score: 826.8
None	No IPR available	PIRSR	PIRSR000290-1	PIRSR000290-1	coord: 72..575 e-value: 6.7E-226 score: 748.8
None	No IPR available	PANTHER	PTHR11474:SF94	POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKE	coord: 4..575
None	No IPR available	PANTHER	PTHR11474	TYROSINASE FAMILY MEMBER	coord: 4..575

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0812.1	MC03g0812.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046148	pigment biosynthetic process
molecular_function	GO:0004097	catechol oxidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity