MC03g0807 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0807
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Epoxide hydrolase-like
Location	MC03: 14739497 .. 14741702 (-)
RNA-Seq Expression	MC03g0807
Synteny	MC03g0807

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTGACATATATATTATTTATTACTCAACGTCGCTCGCACTACACTTTCTTTCTTCCAAATCTACAACAAAAATAGAGAGATCAAAGTCAAAGAAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAATGGATCAGATTCAACACAAGTTCATTGATAGCAAAGGAGTGAAGCTTCACATTGCTGAAATTGGATCTGGTGTTCGCTTCGCCCTCTGTTTTTGAACATCATCAATTGGGTTTCCTTCTTTATTTCATTAATTTTCTCTAATCTCTACAGGCTCAACCGTCGTTGTGTTCGTTCATGGTTTCCCGGAGATATGGTACTCGTGGCGGCATCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCTCCCGATTTAAGGGGCTACGGATTTTCAGACCCTCACCCACAACCTCAAAACGCATCGTTTGATGACTTTGTTCAAGACACTCTGGCAATCCTCGATTTTCTTCACATTCCAAAGGCTTTTCTCGTCGGAAAGGATTTCGGGTCTTGGGTTGTTTACTTGTTTTCTCTCATCTTTCCGGCGAGGGTCGCCGGAATTGTGTCTCTCGGTGTCCCCTTCTTTGCGCCGGATCCAAAACGTTACCAAAATCTTCCTGAGGATTTCTACATTTACCGGTGGAAGGTACTTTCAGTTTCCCGCCCTTCTCTTCGTCCAGATTACAAAATGCAATCGAAATGATTCCTTTTATTTTTCTTCAGGAAACTGGGAGAGCTGAGGCGGACTTCGGAAGATTTGATGTGAAGACTGTACTGAGAAGAATCTACACTCTCTTCGCCAGAAGTGAAATTCCCATCGCTGAGAAAGATCAAGAGATTATGGATATGGTGGACGAATCTACCCCGCTGCCGCCATGGCTGAACGAGGAAGATCTCGCCGCATATGCAACTGCATATCAAAATTCTGGGTTTCAATCTACGTTGCAAGTTCCTTACAGGTATGCAGTTTCTCCCTTTTTTTCCTTGAAAATTTTGACGCAGTGAGGAAAAGAAAGCAAGGAACTGAAAACAGAGAAAATTTGACTGAATTTTAGGAGAAAACATAAAGAGTTGGGCATTACCAATCCGAAGGTTGAAGTTCCAGTTCTGTTGATCATTGGTCGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATATAAAGAGTGAGAAAATGAGGGAGTTTGTGGCAGATTTGGAGGTTGCGGACTTGGCCGATGGAACTCATTTCATGCAGGAGCAGTTTCCAGCTCAACTCAATCACCTTCTCATTGCCTTTTTAAGTAATCATCATATCTGATTACAAGTTTTTATTTTTTCCAAATCCATATTGTTAATGACATGCTATGTTAGTTGACAATATCAAACCAAGTTTCAAAATATTGTTGGGAGTAATGTGCCTGTGAAATGGTCATAAATCAATTTCGAGAAACGTTAGAAATCGAAAAACGTAAGATCAACACAAAGATACCAATGAAGGAAGAAATGGGTTATATAAATTACAAACTCGAAAATGAATAATGATTTCATTCCACTATAGTTTTCGTATACAAGCATCTTACCATGCACTACAATGGTTGTGTAAACGAAAAGGAGAAGAAAATAGTAGAGATAAGAAAGGCTACCAAAAAGAAGAAAAAGAAGAAGAAATCCAGAACTCAATATGGGTGGAGATAGAACTCTTGAGAAAATTTTTATGAAGGAAGAAAACCCTGACTGGTGGAAAAAAGGTACAGAACAATGGTCAATCAGACAACAACATAAGCGATGAGCCCGCTGGTTTCTTCAGTATGTTGCAGCTTTCTATTTCTATGGTATCAATTAAAGATAAGCAATCCGGCTAAGGAGGGTTAAGAATTTACAGGATCTCCATGCTTCAATTATCAGGAGTTTCTACATGATTCGATCACTTCGGTTACAGCTTTATGCGTCTCAGGGGAAATCATGTTCAATGTCAAGTGAAATGCCTGAACAAGAAGAAGAAACTATATTACCGGCACAAGAATATTTAGTCATATTACACAGTTACATTACACTTGTTGAAGCCTTGTTAGTTCGTAAAATGGATGGACATGCAATAAAAAAAAATTGGGAGTAGTTCATGTTTTACTATTAGGAATGGCAAGTCAAAATAAATAAATATACATACCACGAATCGCTC

mRNA sequence

AAATTGACATATATATTATTTATTACTCAACGTCGCTCGCACTACACTTTCTTTCTTCCAAATCTACAACAAAAATAGAGAGATCAAAGTCAAAGAAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAATGGATCAGATTCAACACAAGTTCATTGATAGCAAAGGAGTGAAGCTTCACATTGCTGAAATTGGATCTGGCTCAACCGTCGTTGTGTTCGTTCATGGTTTCCCGGAGATATGGTACTCGTGGCGGCATCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCTCCCGATTTAAGGGGCTACGGATTTTCAGACCCTCACCCACAACCTCAAAACGCATCGTTTGATGACTTTGTTCAAGACACTCTGGCAATCCTCGATTTTCTTCACATTCCAAAGGCTTTTCTCGTCGGAAAGGATTTCGGGTCTTGGGTTGTTTACTTGTTTTCTCTCATCTTTCCGGCGAGGGTCGCCGGAATTGTGTCTCTCGGTGTCCCCTTCTTTGCGCCGGATCCAAAACGTTACCAAAATCTTCCTGAGGATTTCTACATTTACCGGTGGAAGGAAACTGGGAGAGCTGAGGCGGACTTCGGAAGATTTGATGTGAAGACTGTACTGAGAAGAATCTACACTCTCTTCGCCAGAAGTGAAATTCCCATCGCTGAGAAAGATCAAGAGATTATGGATATGGTGGACGAATCTACCCCGCTGCCGCCATGGCTGAACGAGGAAGATCTCGCCGCATATGCAACTGCATATCAAAATTCTGGGTTTCAATCTACGTTGCAAGTTCCTTACAGGAGAAAACATAAAGAGTTGGGCATTACCAATCCGAAGGTTGAAGTTCCAGTTCTGTTGATCATTGGTCGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATATAAAGAGTGAGAAAATGAGGGAGTTTGTGGCAGATTTGGAGGTTGCGGACTTGGCCGATGGAACTCATTTCATGCAGGAGCAGTTTCCAGCTCAACTCAATCACCTTCTCATTGCCTTTTTAAGTAATCATCATATCTGATTACAAGTTTTTATTTTTTCCAAATCCATATTGTTAATGACATGCTATGTTAGTTGACAATATCAAACCAAGTTTCAAAATATTGTTGGGAGTAATGTGCCTGTGAAATGGTCATAAATCAATTTCGAGAAACGTTAGAAATCGAAAAACGTAAGATCAACACAAAGATACCAATGAAGGAAGAAATGGGTTATATAAATTACAAACTCGAAAATGAATAATGATTTCATTCCACTATAGTTTTCGTATACAAGCATCTTACCATGCACTACAATGGTTGTGTAAACGAAAAGGAGAAGAAAATAGTAGAGATAAGAAAGGCTACCAAAAAGAAGAAAAAGAAGAAGAAATCCAGAACTCAATATGGGTGGAGATAGAACTCTTGAGAAAATTTTTATGAAGGAAGAAAACCCTGACTGGTGGAAAAAAGGTACAGAACAATGGTCAATCAGACAACAACATAAGCGATGAGCCCGCTGGTTTCTTCAGTATGTTGCAGCTTTCTATTTCTATGGTATCAATTAAAGATAAGCAATCCGGCTAAGGAGGGTTAAGAATTTACAGGATCTCCATGCTTCAATTATCAGGAGTTTCTACATGATTCGATCACTTCGGTTACAGCTTTATGCGTCTCAGGGGAAATCATGTTCAATGTCAAGTGAAATGCCTGAACAAGAAGAAGAAACTATATTACCGGCACAAGAATATTTAGTCATATTACACAGTTACATTACACTTGTTGAAGCCTTGTTAGTTCGTAAAATGGATGGACATGCAATAAAAAAAAATTGGGAGTAGTTCATGTTTTACTATTAGGAATGGCAAGTCAAAATAAATAAATATACATACCACGAATCGCTC

Coding sequence (CDS)

ATTGACATATATATTATTTATTACTCAACGTCGCTCGCACTACACTTTCTTTCTTCCAAATCTACAACAAAAATAGAGAGATCAAAGTCAAAGAAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAATGGATCAGATTCAACACAAGTTCATTGATAGCAAAGGAGTGAAGCTTCACATTGCTGAAATTGGATCTGGCTCAACCGTCGTTGTGTTCGTTCATGGTTTCCCGGAGATATGGTACTCGTGGCGGCATCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCTCCCGATTTAAGGGGCTACGGATTTTCAGACCCTCACCCACAACCTCAAAACGCATCGTTTGATGACTTTGTTCAAGACACTCTGGCAATCCTCGATTTTCTTCACATTCCAAAGGCTTTTCTCGTCGGAAAGGATTTCGGGTCTTGGGTTGTTTACTTGTTTTCTCTCATCTTTCCGGCGAGGGTCGCCGGAATTGTGTCTCTCGGTGTCCCCTTCTTTGCGCCGGATCCAAAACGTTACCAAAATCTTCCTGAGGATTTCTACATTTACCGGTGGAAGGAAACTGGGAGAGCTGAGGCGGACTTCGGAAGATTTGATGTGAAGACTGTACTGAGAAGAATCTACACTCTCTTCGCCAGAAGTGAAATTCCCATCGCTGAGAAAGATCAAGAGATTATGGATATGGTGGACGAATCTACCCCGCTGCCGCCATGGCTGAACGAGGAAGATCTCGCCGCATATGCAACTGCATATCAAAATTCTGGGTTTCAATCTACGTTGCAAGTTCCTTACAGGAGAAAACATAAAGAGTTGGGCATTACCAATCCGAAGGTTGAAGTTCCAGTTCTGTTGATCATTGGTCGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATATAAAGAGTGAGAAAATGAGGGAGTTTGTGGCAGATTTGGAGGTTGCGGACTTGGCCGATGGAACTCATTTCATGCAGGAGCAGTTTCCAGCTCAACTCAATCACCTTCTCATTGCCTTTTTAAGTAATCATCATATCTGA

Protein sequence

IDIYIIYYSTSLALHFLSSKSTTKIERSKSKKLSLSLSLSLSLSLSLSLSLMDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI

Homology

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BLAST of MC03g0807 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.7e-32
Identity = 92/319 (28.84%), Postives = 165/319 (51.72%), Query Frame = 0


Query: 53  DQIQHKFIDSK-GVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 112
           + + H ++  K G++LH  E+GSG  + +  HGFPE W+SWR+Q+ A A AG+R ++ D+
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSGPALCL-CHGFPESWFSWRYQIPALAQAGFRVLAIDM 292

Query: 113 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 172
           +GYG S   P+ +  + +   ++ +  LD L IP+A  +G D+   +V+  +L +P RV 
Sbjct: 293 KGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVR 352

Query: 173 GIVSLGVPFFAPDP-----KRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLF 232
            + SL  PF  PDP     K  +++P   Y   ++E G AEA+  + ++    +  +   
Sbjct: 353 AVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEK-NMSRTFKSFFRAS 412

Query: 233 ARSEIPIAEKDQEIMDMVDESTPLPPWLN----EEDLAAYATAYQNSGFQSTLQVPYRRK 292
             +      K  EI  ++  +TP  P L+    EE++  Y   ++ +GF+  L   YR  
Sbjct: 413 DETGFIAVHKATEIGGIL-VNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNW-YRNT 472

Query: 293 HKELGIT----NPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGT 352
            +    +      K+ VP L++   KD  L+ P +     S+ M +++  L+   + D  
Sbjct: 473 ERNWKWSCKGLGRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDCG 532

Query: 353 HFMQEQFPAQLNHLLIAFL 358
           H+ Q + P ++N +LI +L
Sbjct: 533 HWTQIEKPTEVNQILIKWL 541

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BLAST of MC03g0807 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 8.7e-31
Identity = 94/324 (29.01%), Postives = 158/324 (48.77%), Query Frame = 0


Query: 55  IQHKFIDSK-GVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 114
           + H ++  K  V+LH  E+GSG  V +  HGFPE WYSWR+Q+ A A AGYR ++ D++G
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGPAVCL-CHGFPESWYSWRYQIPALAQAGYRVLAMDMKG 296

Query: 115 YGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGI 174
           YG S   P+ +    +   ++ +  LD L + +A  +G D+G  +V+  +L +P RV  +
Sbjct: 297 YGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAV 356

Query: 175 VSLGVPFFAPDPKR------YQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFA 234
            SL  PF   +P          N   D+ +Y ++E G AEA+      + + R   +LF 
Sbjct: 357 ASLNTPFIPANPNMSPLESIKANPVFDYQLY-FQEPGVAEAEL----EQNLSRTFKSLFR 416

Query: 235 RSEIPIAEKDQ-----EIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRK 294
            S+  +    +      +     E   L   + EE++  Y   ++ SGF+  L   YR  
Sbjct: 417 ASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNW-YRNM 476

Query: 295 H-------KELGITNPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLA 354
                   K LG    K+ +P L++   KD+ L  P +     S+ M +++  L+   + 
Sbjct: 477 ERNWKWACKSLG---RKILIPALMVTAEKDFVL-VPQM-----SQHMEDWIPHLKRGHIE 536

Query: 355 DGTHFMQEQFPAQLNHLLIAFLSN 360
           D  H+ Q   P ++N +LI +L +
Sbjct: 537 DCGHWTQMDKPTEVNQILIKWLDS 544

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BLAST of MC03g0807 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.3e-30
Identity = 93/322 (28.88%), Postives = 162/322 (50.31%), Query Frame = 0


Query: 53  DQIQHKFIDSK-GVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 112
           + + H ++  K G++LH  E+GSG  + +  HGFPE W+SWR+Q+ A A AG+R ++ D+
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSGPAICL-CHGFPESWFSWRYQIPALAQAGFRVLAIDM 292

Query: 113 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 172
           +GYG S   P+ +  + +   ++ +  L+ L IP+A  +G D+   +V+  +L  P RV 
Sbjct: 293 KGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVR 352

Query: 173 GIVSLGVPFFAPDP-----KRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLF 232
            + SL  P   P+P     +  +++P   Y   ++E G AEA+      K + R   + F
Sbjct: 353 AVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAEL----EKNMSRTFKSFF 412

Query: 233 ARSE---IPIAEKDQEIMDMVDESTPLPPWLN----EEDLAAYATAYQNSGFQSTLQVPY 292
             S+   +    K  E M  +   TP  P ++    EE++  Y   ++ SGF+  L   Y
Sbjct: 413 RTSDDMGLLTVNKATE-MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNW-Y 472

Query: 293 RRKHK----ELGITNPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLA 352
           R   +           K+ VP L++   KD  L+ P +     S+ M  ++  L+   + 
Sbjct: 473 RNTERNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHIE 532

Query: 353 DGTHFMQEQFPAQLNHLLIAFL 358
           D  H+ Q + PA++N +LI +L
Sbjct: 533 DCGHWTQIEKPAEVNQILIKWL 541

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BLAST of MC03g0807 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 9.6e-30
Identity = 93/318 (29.25%), Postives = 152/318 (47.80%), Query Frame = 0


Query: 58  KFIDSKGVKLHIAEIGS-GSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGF 117
           + +D+ GV+L + E G  G+ VV+  HGFPE+ YSWRHQ+ A A AGY  ++PD RGYG 
Sbjct: 7   RLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGG 66

Query: 118 SDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSL 177
           S      +         D + +LD +   +A  VG D+G+ VV+   L+   RVA + +L
Sbjct: 67  SSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAAL 126

Query: 178 GVPFF----APDPKRYQNL--PEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSE 237
            VP       P  + +++      FYI  ++E G A+A+      +T+ R I  L    +
Sbjct: 127 SVPALPRAQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGD 186

Query: 238 IP-----IAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKE 297
                  +A      +D + E   LP W+++E+L  Y   +  +GF   L   YR   + 
Sbjct: 187 QSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW-YRNFDRN 246

Query: 298 ----LGITNPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGT-HF 357
                 +    + VP L I G  D  L F       ++++  E ++      L DG  H+
Sbjct: 247 WETTADLAGKTISVPSLFIAGTADPVLTF------TRTDRAAEVISGPYREVLIDGAGHW 306

Query: 358 MQEQFPAQLNHLLIAFLS 359
           +Q++ P ++   L+ FL+
Sbjct: 307 LQQERPGEVTAALLEFLT 317

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BLAST of MC03g0807 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 9.6e-30
Identity = 94/320 (29.38%), Postives = 158/320 (49.38%), Query Frame = 0


Query: 55  IQHKFIDSK-GVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 114
           + H ++  K GV+LH  E+GSG  V +  HGFPE W+SWR+Q+ A A AG+R ++ D++G
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGPAVCL-CHGFPESWFSWRYQIPALAQAGFRVLAVDMKG 296

Query: 115 YGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGI 174
           YG S   P+ +  S +   +D +  L+ L + +A  +G D+G  +V+  +L +P RV  +
Sbjct: 297 YGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAV 356

Query: 175 VSLGVPFFAPDPK------RYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFA 234
            SL  PF   +P          N   D+ +Y ++E G AEA     +++  L R +  F 
Sbjct: 357 ASLNTPFMPSNPNVSPMEIIKANPVFDYQLY-FQEPGVAEA-----ELEQNLDRTFKNFF 416

Query: 235 RSEIPIAEKDQEIMDM------VDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRR 294
           R+          + ++        E   L   + EED+  Y   ++ SGF+  L   YR 
Sbjct: 417 RAHDETFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRN 476

Query: 295 KHK--ELGI--TNPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADG 354
             +  + G   +  K+ +P L++    D  L  P +     S+ M  ++  L+   + D 
Sbjct: 477 MERNWQWGCKGSGRKILIPALMVTAENDLVL-HPKM-----SKHMENWIPHLKRGHIKDC 536

Query: 355 THFMQEQFPAQLNHLLIAFL 358
            H+ Q   PA+LN +LI +L
Sbjct: 537 GHWTQIDKPAELNRILIEWL 542

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BLAST of MC03g0807 vs. NCBI nr
Match: XP_022140163.1 (uncharacterized protein LOC111010895, partial [Momordica charantia])

HSP 1 Score: 598 bits (1543), Expect = 7.03e-215
Identity = 288/289 (99.65%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 74  SGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ 133
           +GSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ
Sbjct: 1   TGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ 60

Query: 134 DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE 193
           DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE
Sbjct: 61  DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE 120

Query: 194 DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW 253
           DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW
Sbjct: 121 DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW 180

Query: 254 LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI 313
           LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI
Sbjct: 181 LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI 240

Query: 314 EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI 362
           EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI
Sbjct: 241 EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI 289

[Close]

BLAST of MC03g0807 vs. NCBI nr
Match: XP_038905360.1 (bifunctional epoxide hydrolase 2-like [Benincasa hispida])

HSP 1 Score: 553 bits (1424), Expect = 2.14e-196
Identity = 258/311 (82.96%), Postives = 286/311 (91.96%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           MDQI H F+  K VKLHIAEIG+GSTVVVF+HGFPEIWYSWRHQMVAAAAAGYRAI PDL
Sbjct: 1   MDQIHHNFVTIKEVKLHIAEIGTGSTVVVFLHGFPEIWYSWRHQMVAAAAAGYRAICPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYGFSDPHP PQNASF+DFV+D + ILDFLHIPKAFLVGKDFGSWVVYLFSL +PARVA
Sbjct: 61  RGYGFSDPHPHPQNASFEDFVEDIVGILDFLHIPKAFLVGKDFGSWVVYLFSLFYPARVA 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G+VSLGVPF  P+P RY++LPE FYI+RWKE+GRAEADFGRF+VKTVL RIYTLF+RSEI
Sbjct: 121 GVVSLGVPFLPPNPMRYRDLPEGFYIFRWKESGRAEADFGRFNVKTVLSRIYTLFSRSEI 180

Query: 232 PIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNP 291
           PIAEKD+EIMDMVDESTP PPWL +EDL  Y T Y++SGF+S LQVPYRRKHKELGI+NP
Sbjct: 181 PIAEKDKEIMDMVDESTPRPPWLTDEDLTVYTTLYEHSGFESALQVPYRRKHKELGISNP 240

Query: 292 KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNH 351
           +VEVPVLLIIG KDYFLKFPG+EDYIKSEK+REF+ADLEVADLADGTHFMQEQFPAQ+NH
Sbjct: 241 RVEVPVLLIIGGKDYFLKFPGVEDYIKSEKLREFMADLEVADLADGTHFMQEQFPAQVNH 300

Query: 352 LLIAFLSNHHI 362
           LLI FL+ H+I
Sbjct: 301 LLINFLTKHNI 311

[Close]

BLAST of MC03g0807 vs. NCBI nr
Match: PON96090.1 (Epoxide hydrolase-like [Trema orientale])

HSP 1 Score: 490 bits (1262), Expect = 1.06e-171
Identity = 223/310 (71.94%), Postives = 267/310 (86.13%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           MDQIQHK+ID++GVKLHIAEIG+GST VVF+HGFPEIWYSWRHQM+A A AGYRAI+PDL
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGSTAVVFLHGFPEIWYSWRHQMIAVAQAGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG S+PHP+PQ ASF+DFV+DTL+ILDF +I KAFLVGKDFGSW VYLF L +P RVA
Sbjct: 61  RGYGLSEPHPEPQKASFNDFVEDTLSILDFFNIEKAFLVGKDFGSWPVYLFCLTYPTRVA 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G++SLGVPFF PDP+RY+N+PE FYI+RWKE GRAEADFGRFDVKTVLR IY LF+RSEI
Sbjct: 121 GVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDVKTVLRNIYILFSRSEI 180

Query: 232 PIAEKDQEIMDMVD-ESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITN 291
           PIAEKD+E MD+VD ++TPLPPW  EEDL+ YAT YQ SGF S +QVPY+   +E  I  
Sbjct: 181 PIAEKDKETMDLVDSDNTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFRIAK 240

Query: 292 PKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLN 351
           PK+EVP ++I+G KDYFLKFPGIE+Y K+ KMR++  + E+  LA+GTHFMQEQFPAQ+N
Sbjct: 241 PKIEVPAMVIMGGKDYFLKFPGIEEYTKNGKMRDYAPNSEMKFLAEGTHFMQEQFPAQVN 300

Query: 352 HLLIAFLSNH 360
            L+I FL++H
Sbjct: 301 QLIITFLNDH 310

[Close]

BLAST of MC03g0807 vs. NCBI nr
Match: XP_015890165.1 (bifunctional epoxide hydrolase 2-like [Ziziphus jujuba])

HSP 1 Score: 488 bits (1255), Expect = 1.18e-170
Identity = 232/311 (74.60%), Postives = 262/311 (84.24%), Query Frame = 0


Query: 52  MDQIQHKFI--DSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISP 111
           MDQIQHK+I  DS GVKLHIAEIG+GS VVVF+HGFPEIWYSWRHQM+A A AGYRAISP
Sbjct: 1   MDQIQHKYIYSDSGGVKLHIAEIGTGSKVVVFIHGFPEIWYSWRHQMIAVAKAGYRAISP 60

Query: 112 DLRGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPAR 171
           DLRGYG S PHP  QNASF+DFVQD LAILD L+I KAFLVGKDFGSW VYLFSL  P R
Sbjct: 61  DLRGYGLSHPHPNLQNASFNDFVQDILAILDSLNIHKAFLVGKDFGSWPVYLFSLTHPER 120

Query: 172 VAGIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARS 231
           V+GI+SLGVPF  P+P+RY++LPE FYI RWKE GRAEADF RFDVKT++R IY LF+RS
Sbjct: 121 VSGIISLGVPFLPPNPQRYKDLPEGFYISRWKEAGRAEADFSRFDVKTIVRNIYILFSRS 180

Query: 232 EIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGIT 291
           EIPIA+KD+EIMDMVD STPLPPW   EDLA YAT Y+NSGF S +QVPY+   KE  IT
Sbjct: 181 EIPIAKKDEEIMDMVDSSTPLPPWFTNEDLATYATLYENSGFDSPMQVPYKGLRKESVIT 240

Query: 292 NPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQL 351
           N K+E PVLL++G KDYFLKFPGIE+Y+KS KMRE+V DLEV  LADGTHFMQEQ P Q+
Sbjct: 241 NLKIEAPVLLVMGGKDYFLKFPGIEEYVKSGKMREYVGDLEVKFLADGTHFMQEQLPEQV 300

Query: 352 NHLLIAFLSNH 360
           N L+++FLS H
Sbjct: 301 NQLIVSFLSQH 311

[Close]

BLAST of MC03g0807 vs. NCBI nr
Match: PON36798.1 (Epoxide hydrolase-like [Parasponia andersonii])

HSP 1 Score: 487 bits (1253), Expect = 2.47e-170
Identity = 222/310 (71.61%), Postives = 263/310 (84.84%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           MDQIQHK+ID++GVKLHIAEIG+GST VVF+HGFPEIWYSWRHQM+A A AGYRAI+PDL
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGSTAVVFLHGFPEIWYSWRHQMIAIAQAGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG S+PHP+PQ ASF+DFV+DTL+ILDF  I KAFLVGKDFGSW VYLF L +P RVA
Sbjct: 61  RGYGLSEPHPEPQKASFNDFVEDTLSILDFFKIEKAFLVGKDFGSWPVYLFCLTYPTRVA 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G++SLGVPFF PDP+RY+N+PE FYI+RWKE GRAEADFGRFD++TVLR IY LF+RSEI
Sbjct: 121 GVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDMRTVLRNIYILFSRSEI 180

Query: 232 PIAEKDQEIMDMVDE-STPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITN 291
           PIAE D+EIMD+VD   TPLPPW  EEDL+ YAT YQ SGF S +QVPY+   +E GI  
Sbjct: 181 PIAETDKEIMDLVDSHDTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFGIAK 240

Query: 292 PKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLN 351
           PK+EVP +LI+G KDYFLKFPGIE+Y K+  MR++V + E   LA+GTHFMQEQFP Q+N
Sbjct: 241 PKIEVPAMLIMGGKDYFLKFPGIEEYTKNGNMRDYVPNSETKFLAEGTHFMQEQFPEQVN 300

Query: 352 HLLIAFLSNH 360
            L+I FL++H
Sbjct: 301 QLIITFLNDH 310

[Close]

BLAST of MC03g0807 vs. ExPASy TrEMBL
Match: A0A6J1CHD9 (uncharacterized protein LOC111010895 OS=Momordica charantia OX=3673 GN=LOC111010895 PE=4 SV=1)

HSP 1 Score: 598 bits (1543), Expect = 3.40e-215
Identity = 288/289 (99.65%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 74  SGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ 133
           +GSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ
Sbjct: 1   TGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFVQ 60

Query: 134 DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE 193
           DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE
Sbjct: 61  DTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLPE 120

Query: 194 DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW 253
           DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW
Sbjct: 121 DFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPW 180

Query: 254 LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI 313
           LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI
Sbjct: 181 LNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPGI 240

Query: 314 EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI 362
           EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI
Sbjct: 241 EDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHHI 289

[Close]

BLAST of MC03g0807 vs. ExPASy TrEMBL
Match: A0A2P5FE57 (Epoxide hydrolase-like OS=Trema orientale OX=63057 GN=TorRG33x02_080200 PE=4 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 5.11e-172
Identity = 223/310 (71.94%), Postives = 267/310 (86.13%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           MDQIQHK+ID++GVKLHIAEIG+GST VVF+HGFPEIWYSWRHQM+A A AGYRAI+PDL
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGSTAVVFLHGFPEIWYSWRHQMIAVAQAGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG S+PHP+PQ ASF+DFV+DTL+ILDF +I KAFLVGKDFGSW VYLF L +P RVA
Sbjct: 61  RGYGLSEPHPEPQKASFNDFVEDTLSILDFFNIEKAFLVGKDFGSWPVYLFCLTYPTRVA 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G++SLGVPFF PDP+RY+N+PE FYI+RWKE GRAEADFGRFDVKTVLR IY LF+RSEI
Sbjct: 121 GVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDVKTVLRNIYILFSRSEI 180

Query: 232 PIAEKDQEIMDMVD-ESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITN 291
           PIAEKD+E MD+VD ++TPLPPW  EEDL+ YAT YQ SGF S +QVPY+   +E  I  
Sbjct: 181 PIAEKDKETMDLVDSDNTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFRIAK 240

Query: 292 PKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLN 351
           PK+EVP ++I+G KDYFLKFPGIE+Y K+ KMR++  + E+  LA+GTHFMQEQFPAQ+N
Sbjct: 241 PKIEVPAMVIMGGKDYFLKFPGIEEYTKNGKMRDYAPNSEMKFLAEGTHFMQEQFPAQVN 300

Query: 352 HLLIAFLSNH 360
            L+I FL++H
Sbjct: 301 QLIITFLNDH 310

[Close]

BLAST of MC03g0807 vs. ExPASy TrEMBL
Match: A0A6P4AMV9 (bifunctional epoxide hydrolase 2-like OS=Ziziphus jujuba OX=326968 GN=LOC107424812 PE=4 SV=1)

HSP 1 Score: 488 bits (1255), Expect = 5.73e-171
Identity = 232/311 (74.60%), Postives = 262/311 (84.24%), Query Frame = 0


Query: 52  MDQIQHKFI--DSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISP 111
           MDQIQHK+I  DS GVKLHIAEIG+GS VVVF+HGFPEIWYSWRHQM+A A AGYRAISP
Sbjct: 1   MDQIQHKYIYSDSGGVKLHIAEIGTGSKVVVFIHGFPEIWYSWRHQMIAVAKAGYRAISP 60

Query: 112 DLRGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPAR 171
           DLRGYG S PHP  QNASF+DFVQD LAILD L+I KAFLVGKDFGSW VYLFSL  P R
Sbjct: 61  DLRGYGLSHPHPNLQNASFNDFVQDILAILDSLNIHKAFLVGKDFGSWPVYLFSLTHPER 120

Query: 172 VAGIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARS 231
           V+GI+SLGVPF  P+P+RY++LPE FYI RWKE GRAEADF RFDVKT++R IY LF+RS
Sbjct: 121 VSGIISLGVPFLPPNPQRYKDLPEGFYISRWKEAGRAEADFSRFDVKTIVRNIYILFSRS 180

Query: 232 EIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGIT 291
           EIPIA+KD+EIMDMVD STPLPPW   EDLA YAT Y+NSGF S +QVPY+   KE  IT
Sbjct: 181 EIPIAKKDEEIMDMVDSSTPLPPWFTNEDLATYATLYENSGFDSPMQVPYKGLRKESVIT 240

Query: 292 NPKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQL 351
           N K+E PVLL++G KDYFLKFPGIE+Y+KS KMRE+V DLEV  LADGTHFMQEQ P Q+
Sbjct: 241 NLKIEAPVLLVMGGKDYFLKFPGIEEYVKSGKMREYVGDLEVKFLADGTHFMQEQLPEQV 300

Query: 352 NHLLIAFLSNH 360
           N L+++FLS H
Sbjct: 301 NQLIVSFLSQH 311

[Close]

BLAST of MC03g0807 vs. ExPASy TrEMBL
Match: A0A2P5AJS1 (Epoxide hydrolase-like OS=Parasponia andersonii OX=3476 GN=PanWU01x14_325590 PE=4 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 1.20e-170
Identity = 222/310 (71.61%), Postives = 263/310 (84.84%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           MDQIQHK+ID++GVKLHIAEIG+GST VVF+HGFPEIWYSWRHQM+A A AGYRAI+PDL
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGSTAVVFLHGFPEIWYSWRHQMIAIAQAGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG S+PHP+PQ ASF+DFV+DTL+ILDF  I KAFLVGKDFGSW VYLF L +P RVA
Sbjct: 61  RGYGLSEPHPEPQKASFNDFVEDTLSILDFFKIEKAFLVGKDFGSWPVYLFCLTYPTRVA 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G++SLGVPFF PDP+RY+N+PE FYI+RWKE GRAEADFGRFD++TVLR IY LF+RSEI
Sbjct: 121 GVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDMRTVLRNIYILFSRSEI 180

Query: 232 PIAEKDQEIMDMVDE-STPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITN 291
           PIAE D+EIMD+VD   TPLPPW  EEDL+ YAT YQ SGF S +QVPY+   +E GI  
Sbjct: 181 PIAETDKEIMDLVDSHDTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFGIAK 240

Query: 292 PKVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLN 351
           PK+EVP +LI+G KDYFLKFPGIE+Y K+  MR++V + E   LA+GTHFMQEQFP Q+N
Sbjct: 241 PKIEVPAMLIMGGKDYFLKFPGIEEYTKNGNMRDYVPNSETKFLAEGTHFMQEQFPEQVN 300

Query: 352 HLLIAFLSNH 360
            L+I FL++H
Sbjct: 301 QLIITFLNDH 310

[Close]

BLAST of MC03g0807 vs. ExPASy TrEMBL
Match: A0A1R3H476 (Epoxide hydrolase-like protein OS=Corchorus olitorius OX=93759 GN=COLO4_31508 PE=4 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 1.69e-163
Identity = 214/309 (69.26%), Postives = 255/309 (82.52%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           M+QIQHK++D +GVKLH+AEIG+GS+VVVF+HGFPEIWYSWRHQM+A A AGYRAI+PDL
Sbjct: 1   MEQIQHKYVDVRGVKLHVAEIGTGSSVVVFLHGFPEIWYSWRHQMIAIANAGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG S  HP P+ ASF+DFV DTLAILD  HI KAFL+GKDFGSW VYL SL+ P R+ 
Sbjct: 61  RGYGLSQHHPDPEKASFNDFVDDTLAILDSFHIHKAFLIGKDFGSWPVYLLSLLHPTRIL 120

Query: 172 GIVSLGVPFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEI 231
           G+VSLGVPFF P P+RYQ LPE FYI+RWKE GRAEADF RFD+KT+ R IY LF+RSEI
Sbjct: 121 GVVSLGVPFFIPRPQRYQQLPEGFYIFRWKEPGRAEADFRRFDIKTIWRNIYILFSRSEI 180

Query: 232 PIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNP 291
           PIA+K +EIMD+V+ STPLP WLN+ED   YA AY+ SGF S + VPY+ + +E  I NP
Sbjct: 181 PIADKGKEIMDLVNPSTPLPTWLNDEDFTTYAKAYEQSGFSSPMHVPYKNRREEFTIENP 240

Query: 292 KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNH 351
           KVEVPVLLI+G KDYFLKFPGIE+YI S K+R++VA+L +  L DGTHFMQEQFP ++N 
Sbjct: 241 KVEVPVLLIVGEKDYFLKFPGIEEYITSGKVRDYVAELYINFLPDGTHFMQEQFPDKVNQ 300

Query: 352 LLIAFLSNH 360
           L+I FL  H
Sbjct: 301 LIIDFLDKH 309

[Close]

BLAST of MC03g0807 vs. TAIR 10
Match: AT3G51000.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.3e-50
Identity = 108/319 (33.86%), Postives = 178/319 (55.80%), Query Frame = 0


Query: 51  LMDQIQHKFIDSKGVKLHIAEIG-SGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISP 110
           +   ++ K I + G+ L++AE G     +V+ +HGFPE WYSWRHQ+   ++ GY  ++P
Sbjct: 1   MTSSVREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAP 60

Query: 111 DLRGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPAR 170
           DLRGYG SD  P  ++ +    V D + +LD     +AF+ G D+G+ + +   L  P R
Sbjct: 61  DLRGYGDSDSLPSHESYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDR 120

Query: 171 VAGIVSLGVPFFAPDPKR-----YQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIYT 230
           V G +SL VP+F  DPK      ++   +  YI ++++ GRAEA F + D  +V+++ + 
Sbjct: 121 VKGFISLSVPYFPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMKK-FL 180

Query: 231 LFARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHK 290
           L  R++  +A  D EI+D ++  + +P W+ EE++  YA  +Q SGF   L   YR    
Sbjct: 181 LITRTDYLVAPPDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDM 240

Query: 291 ELGITNP----KVEVPVLLIIGRKDYFLKFP-GIEDYIKSEKMREFVADLEVADLADGTH 350
              I  P    K+ VP   I G KD   + P G  +Y+K E  +  V +LE+  +  G H
Sbjct: 241 NWEILAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHH 300

Query: 351 FMQEQFPAQLNHLLIAFLS 359
           F+Q++   Q++  +++FL+
Sbjct: 301 FIQQEKSEQVSQEILSFLN 317

[Close]

BLAST of MC03g0807 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.9e-49
Identity = 110/316 (34.81%), Postives = 175/316 (55.38%), Query Frame = 0


Query: 52  MDQIQHKFIDSKGVKLHIAEIGSGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDL 111
           M++I+H  I + G+ +H+A IGSG  V++FVHGFP++WYSWRHQ+V+ AA GYRAI+PDL
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSG-PVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDL 60

Query: 112 RGYGFSDPHPQPQNASFDDFVQDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVA 171
           RGYG SD  P  ++ +    V D + +LD L + + FLVG D+G+ V +   +I P RV 
Sbjct: 61  RGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVN 120

Query: 172 GIVSLGVPFFAPDPK-----RYQNL-PEDFYIYRWKETGRAEADFGRFDVKTVLRRIYTL 231
            +V+  V F   +P       ++ L  +D+YI R++E G  E DF + D K ++ R +T 
Sbjct: 121 ALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFT- 180

Query: 232 FARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKE 291
            +R+  P           + +   LP WL E+D+  Y   +   GF   L   YR  +  
Sbjct: 181 -SRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALNLS 240

Query: 292 LGITNP----KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADGT-HF 351
             +T P    +++VPV  I+G  D     PG ++YI    +++ V  L+   + +G  HF
Sbjct: 241 WELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHF 300

Query: 352 MQEQFPAQLNHLLIAF 357
           + ++ P ++   +  F
Sbjct: 301 LHQEKPDEVTDHIYGF 312

[Close]

BLAST of MC03g0807 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 186.8 bits (473), Expect = 3.1e-47
Identity = 106/321 (33.02%), Postives = 175/321 (54.52%), Query Frame = 0


Query: 55  IQHKFIDSKGVKLHIAEIG-SGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 114
           ++H+ +   G+ +H+A  G S   +V+ +HGFPE+WYSWRHQ+   AA GYRA++PDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 115 YGFSDPHPQPQNASFDDFVQDTLAILDFL---HIPKAFLVGKDFGSWVVYLFSLIFPARV 174
           YG SD   +  + +  + V D +A++  L      K F+VG D+G+ + +   L  P RV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 175 AGIVSLGVPF-FAP-DP------KRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRI 234
             +V+L VPF F P DP      +      +D+YI R++E G  EA+      + V++R+
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 235 YTLFARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRK 294
            T      + I  KD+       E+ PLP WL EED+A + + ++  GF   +   YR  
Sbjct: 181 LTYRTPGPV-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNF 240

Query: 295 HKELGITNP----KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADG- 354
           ++   +  P    K++VP   +IG  D     PG+++YI   + +E V  LE   + +G 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 355 THFMQEQFPAQLNHLLIAFLS 359
            HF+ ++ P ++  +++ F+S
Sbjct: 301 AHFINQEKPQEILQIILDFIS 319

[Close]

BLAST of MC03g0807 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.0e-46
Identity = 102/320 (31.87%), Postives = 174/320 (54.37%), Query Frame = 0


Query: 55  IQHKFIDSKGVKLHIAEIG-SGSTVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 114
           ++H+ +   G+ +H+A  G S  T+V+ +HGFPE+WYSWRHQ+   AA GYRA++PDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 115 YGFSDPHPQPQNASFDDFVQDTLAILDFL--HIPKAFLVGKDFGSWVVYLFSLIFPARVA 174
           YG SD   +  + +  + V D +A++  L     K F+VG D+G+ + +   L  P +V 
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 175 GIVSLGVP--FFAPDP------KRYQNLPEDFYIYRWKETGRAEADFGRFDVKTVLRRIY 234
            +V+L VP  F+  DP      +       D+Y+ R++E G  EA+      + V++R+ 
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 235 TLFARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQVPYRRKH 294
           T      + I  KD+       E+ PLP WL EED+A + + ++  GF   +   YR  +
Sbjct: 181 TYRTPGPL-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNY-YRNFN 240

Query: 295 KELGITNP----KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVADLADG-T 354
           +   +  P    K++VP   +IG  D     PG+++YI   + +E V  +E   + +G  
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 355 HFMQEQFPAQLNHLLIAFLS 359
           HF+ ++ P ++  +++ F+S
Sbjct: 301 HFLNQEKPQEILQIILDFIS 318

[Close]

BLAST of MC03g0807 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.0e-43
Identity = 101/318 (31.76%), Postives = 169/318 (53.14%), Query Frame = 0


Query: 50  SLMDQIQHKFIDSKGVKLHIAE-IGSGS---TVVVFVHGFPEIWYSWRHQMVAAAAAGYR 109
           S +D ++HK +   G+ +H+AE  GSGS    +++F+HGFPE+WY+WRHQMVA ++ GYR
Sbjct: 49  SRLDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYR 108

Query: 110 AISPDLRGYGFSDPHPQPQNASFDDFVQDTLAILDFL--HIPKAFLVGKDFGSWVVYLFS 169
            I+PDLRGYG ++   + ++ ++ +   D +A++D +        +VG D+G+ + +   
Sbjct: 109 TIAPDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLC 168

Query: 170 LIFPARVAGIVSLGV------PFFAPDPKRYQNLPEDFYIYRWKETGRAEADFGRFDVKT 229
              P +V  +V++ V      P   P P       +D+Y+ R+++ G  E +F +   + 
Sbjct: 169 QYRPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTEN 228

Query: 230 VLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPPWLNEEDLAAYATAYQNSGFQSTLQV 289
           VL+   T      + +  KD+      + ++ LP WL +EDL  Y T Y+N GF   +  
Sbjct: 229 VLKEFLTYKTPGPLNL-PKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINY 288

Query: 290 PYRRKHKELGITNP----KVEVPVLLIIGRKDYFLKFPGIEDYIKSEKMREFVADLEVAD 349
            YR   +   +T P    K+ VPV  IIG +D    FPG ++YI     +  V  L+   
Sbjct: 289 -YRNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETV 348

Query: 350 LADGT-HFMQEQFPAQLN 351
           +  G  HF+ E+ P  +N
Sbjct: 349 VLKGLGHFLHEENPDVIN 364

The following BLAST results are available for this feature:

BLAST of MC03g0807 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34914	2.7e-32	28.84	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]
P34913	8.7e-31	29.01	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
P80299	3.3e-30	28.88	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=...	[more]
I6YGS0	9.6e-30	29.25	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]
Q6Q2C2	9.6e-30	29.38	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]

BLAST of MC03g0807 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140163.1	7.03e-215	99.65	uncharacterized protein LOC111010895, partial [Momordica charantia]	[more]
XP_038905360.1	2.14e-196	82.96	bifunctional epoxide hydrolase 2-like [Benincasa hispida]	[more]
PON96090.1	1.06e-171	71.94	Epoxide hydrolase-like [Trema orientale]	[more]
XP_015890165.1	1.18e-170	74.60	bifunctional epoxide hydrolase 2-like [Ziziphus jujuba]	[more]
PON36798.1	2.47e-170	71.61	Epoxide hydrolase-like [Parasponia andersonii]	[more]

BLAST of MC03g0807 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHD9	3.40e-215	99.65	uncharacterized protein LOC111010895 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A2P5FE57	5.11e-172	71.94	Epoxide hydrolase-like OS=Trema orientale OX=63057 GN=TorRG33x02_080200 PE=4 SV=...	[more]
A0A6P4AMV9	5.73e-171	74.60	bifunctional epoxide hydrolase 2-like OS=Ziziphus jujuba OX=326968 GN=LOC1074248...	[more]
A0A2P5AJS1	1.20e-170	71.61	Epoxide hydrolase-like OS=Parasponia andersonii OX=3476 GN=PanWU01x14_325590 PE=...	[more]
A0A1R3H476	1.69e-163	69.26	Epoxide hydrolase-like protein OS=Corchorus olitorius OX=93759 GN=COLO4_31508 PE...	[more]

BLAST of MC03g0807 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51000.1	2.3e-50	33.86	alpha/beta-Hydrolases superfamily protein	[more]
AT4G02340.1	1.9e-49	34.81	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	3.1e-47	33.02	soluble epoxide hydrolase	[more]
AT2G26750.1	2.0e-46	31.88	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	2.0e-43	31.76	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 149..162 score: 29.8 coord: 103..118 score: 44.61 coord: 163..176 score: 23.65
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 78..305 e-value: 4.5E-20 score: 72.4
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 83..101 score: 52.87 coord: 149..162 score: 27.92 coord: 295..311 score: 28.61 coord: 103..118 score: 57.67 coord: 334..356 score: 24.51 coord: 163..176 score: 31.49
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 52..361 e-value: 8.5E-81 score: 274.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 51..359
None	No IPR available	PANTHER	PTHR43329:SF93	BIFUNCTIONAL EPOXIDE HYDROLASE 2-LIKE	coord: 53..360
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 53..360

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0807.1	MC03g0807.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004715	non-membrane spanning protein tyrosine kinase activity
molecular_function	GO:0003824	catalytic activity