Homology
BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match:
Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)
HSP 1 Score: 698.0 bits (1800), Expect = 9.5e-200
Identity = 343/590 (58.14%), Postives = 438/590 (74.24%), Query Frame = 0
Query: 3 NLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIP 62
+L EK+K LQ +EDVT AD++QRRVL EILS NA VEYL++HGL G TD TFK+++P
Sbjct: 16 SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75
Query: 63 IVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISN 122
+V+YE ++P I+RI NGD S +LC++PI+EF TS+GTSGGE+KL+PTIE+E R+ + +
Sbjct: 76 VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135
Query: 123 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSY 182
+ P M Q PGL GK M+F F K ES T G+ AR V TS+ KS H N + ++Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195
Query: 183 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 242
TSP + C+D YQS+Y Q+LCGLCQ+++V+RVG++FAS I KFLE HW LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255
Query: 243 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 302
TGT++++I DS VR++V ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ +VTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315
Query: 303 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 362
+MS YIP L+YY LPL+ YASSEC+FG+NL PLCKP E+SYT IP +AYFEFLPV
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375
Query: 363 RTND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFK 422
R + + + QE VDLVDVKLGQEYE+VVTTYAGLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435
Query: 423 NKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPG 482
N AP F F+ RKNV+LS+D +KTDE EL NAV + L F A L +YTSYAD+S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495
Query: 483 HYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTF 542
HYVL+WE+ + IP S++EDCCL IEESL+SVYR+ R K I PLEIK+VE+ TF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555
Query: 543 DKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
DKLM+ + GASI+QYKTPRCVK IIE L+S+VV +YFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604
BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match:
O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)
HSP 1 Score: 694.9 bits (1792), Expect = 8.0e-199
Identity = 340/589 (57.72%), Postives = 438/589 (74.36%), Query Frame = 0
Query: 4 LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
LD+K+KQ LQ +E++T AD++QR+VL EIL+ NA VEYL++H LNG TD TFKN++P+
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76
Query: 64 VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
++YE ++P I+RI NGD SPIL + PI+EF TS+GTSGGE+KL+PTIE+E R+ + +
Sbjct: 77 ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136
Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYT 183
+ P M Q PGL NGK M+F F K ES T G+ AR V TS+ KS H + ++YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196
Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
SP + + C+D YQS+Y Q+LCGLCQ+Q+V+RVG++FAS I KFLE HW+ L DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256
Query: 244 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 303
GT+++ I D VR++V ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ +VTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316
Query: 304 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 363
MS YIP L+YY LPL+ YASSEC+FG+NL PLCKP E+SYT IP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376
Query: 364 TND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKN 423
N + + QE VDLVDVKLGQEYE+VVTTYAGL RY +GD++RVTGFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436
Query: 424 KAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGH 483
KAP F F+ RKNV+LS+D +KTDE EL NAV + L F A L +YTSYAD+S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496
Query: 484 YVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFD 543
YVL+WE+ + + IP S++EDCCLA+EES ++VYR+ R K I PLEIKIVE TFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556
Query: 544 KLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
KLM+ + GASI+QYKTPRCVK IIE L+S+VV +YFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604
BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match:
Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)
HSP 1 Score: 617.5 bits (1591), Expect = 1.6e-175
Identity = 301/574 (52.44%), Postives = 407/574 (70.91%), Query Frame = 0
Query: 10 QALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTD-ASTFKNLIPIVSYEH 69
+AL+ +E VT A ++QRRVL EIL+ NA EYL+++G+ GS D F+ L+P+V+YE
Sbjct: 22 EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81
Query: 70 LKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMRPRM 129
L+P I RI NGD SPI PI+EF TS+GTSGGE+KL+PTI E R+ + + + P M
Sbjct: 82 LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141
Query: 130 KQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSPTDI 189
Q GL GK M+ F K ES T G+ AR V TS+ +S + + +SYTSP +
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201
Query: 190 LCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTINA 249
+ C D YQS+Y QLLCGL V+RVG++FAS + FLE HW L +DIRTG ++
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261
Query: 250 KIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSHYI 309
+I D VR +V +L+ DP LAD IE EC++ +WEGI+ RLWP TK+I+ +VTG+MS YI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321
Query: 310 PQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTNDSG 369
P L +Y LPL YASSEC+FG+NL+P+CKP +++YT IPT+ Y+EFLPV+ N +
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381
Query: 370 EVG-QEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVIL 429
E ++ VDLVDVKLG EYE+VVTTY+GLYRY +GD++RV GFKNKAP F FV R+NV L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441
Query: 430 SLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEISI 489
S+D +KTDE ELH AV+ L+ FGA LV+YTSYAD++T PGHYVL+WE+ + +
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501
Query: 490 IPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASISQ 549
+P+S++E+CCL++EE+L+SVYR+ R+ + I PLEI++V TFDKLM+ + +GASI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561
Query: 550 YKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
YK PRCV+ ++E LD++V YFS K PKW P
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 593
BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match:
Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)
HSP 1 Score: 607.4 bits (1565), Expect = 1.7e-172
Identity = 286/580 (49.31%), Postives = 407/580 (70.17%), Query Frame = 0
Query: 6 EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
E + +AL+ +E++TR D +Q +VL EILS N++ EYL++ LNG+ D +FK+ +P+V
Sbjct: 18 ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77
Query: 66 YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
YE LK I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++ R+ + N +
Sbjct: 78 YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137
Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSPTD 185
P M PGL GK ++F F K ES T G+ AR TS+ KS + +S YTSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197
Query: 186 ILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTIN 245
+ C D QS+Y Q+LCGL +V R+G++F S L+ FL+N+W L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257
Query: 246 AKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSH 305
+KI D ++ + NIL KPD +LA+F+ CS+ WEGI+T++WPNTK+++ +VTG+M+
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317
Query: 306 YIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTND 365
YIP L YY LP+ ++ YASSE +FGINL+P+CKP E+SYT P +AYFEFLP + D
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377
Query: 366 SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVI 425
G V+L DV++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+ R+NV+
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437
Query: 426 LSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEIS 485
LS++ +KTDEA+L AV LL+ G +++YTSYAD+ T PGHYV+YWE+ +++ +
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497
Query: 486 IIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 545
+PS + CCL +EESL++VYR++R K I PLEI++V+ TF++LM+ + +G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557
Query: 546 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 584
+QYK PRCV T I++ LDS+VV+ +FS P W P R H
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597
BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match:
A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)
HSP 1 Score: 595.1 bits (1533), Expect = 8.6e-169
Identity = 284/579 (49.05%), Postives = 398/579 (68.74%), Query Frame = 0
Query: 6 EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
E D + L+ ++++T D +Q RVL EIL NA EYL + GL+G+TD + F+ +P+VS
Sbjct: 24 EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83
Query: 66 YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
Y+ L+PYI RI NGD SPIL HP++EF TS+GTS GE+KL+PTI E R+ + + +
Sbjct: 84 YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143
Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
P M PGL GK ++F F K E+ T G+ AR V TS+ KS H N + +YTSP
Sbjct: 144 PVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSP 203
Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
T + C D +QS+Y Q++CGLCQ V+R+G++FAS L+ +FL+ +W LA+DI +G
Sbjct: 204 TAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGE 263
Query: 246 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 305
+ ++ D VR++V IL PDP+LA I +ECSKG W GI+TR+WPNTK+++ +VTG+M+
Sbjct: 264 LTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMA 323
Query: 306 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 365
YIP L +Y LP+ YASSEC+FG+NL P+C P E+SYT +P + YFEFLPVD T
Sbjct: 324 QYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG 383
Query: 366 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 425
+ + VDL V++G+EYE+V+TTYAGL RY +GD++RVTGF N AP FRFV RKNV
Sbjct: 384 AASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNV 443
Query: 426 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK-EE 485
+LS++ +KTDEAEL AV + +LL GA +V+YTS A + PGHYV+YWE+ K
Sbjct: 444 LLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAG 503
Query: 486 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 545
+++ + CCL +EE+L++VYR++R I PLEI++V TF++LM+ + +GAS
Sbjct: 504 ATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGAS 563
Query: 546 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
I+QYK PRCV I+E LDS+VV+++FS P W P R
Sbjct: 564 INQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602
BLAST of MC02g_new0098 vs. NCBI nr
Match:
XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])
HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0
Query: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL
Sbjct: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
Query: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI
Sbjct: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
BLAST of MC02g_new0098 vs. NCBI nr
Match:
XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])
HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 433/574 (75.44%), Postives = 496/574 (86.41%), Query Frame = 0
Query: 4 LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
LD KDKQ L +ED T ADEIQRRV+TEILS NAHVEYL+Q GL+GSTD STFK LIP+
Sbjct: 6 LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65
Query: 64 VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
VSYE LKPYIDRI +GDDSPILCA IT+F STGTSGG QKLIP + E R+L+ + Y
Sbjct: 66 VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125
Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183
P MKQLFPGL NGKEMFFNF + E+ TK G+VA+ +S+ K ++V+V +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185
Query: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243
DI+ CTDVYQSLYCQLLCGLCQNQ V+RVG+LFASTLIYIFKFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245
Query: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303
++AKI D VR+SVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ VVTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305
Query: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363
YIPQLN+YCK P+++VFYASSECFFGINLDPLCKPEEISYTF+PT+AYFEFLPVDR N
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365
Query: 364 -DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423
++GEVGQE VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNK PNF F RKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425
Query: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483
VILSLD+EKTDE +LH+AV + LLS GAI+VDYTSYADSSTFPGHYVLYWEIQ +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485
Query: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543
S IPS++YEDCCLA+EESLSSVYR+ARS+ KVINPLEIKIVE TF+KLME+ VD+GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545
Query: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPK 576
ISQYKTPRC+KSTQ+IE LDSKVV+NYFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578
BLAST of MC02g_new0098 vs. NCBI nr
Match:
XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])
HSP 1 Score: 856 bits (2212), Expect = 1.24e-308
Identity = 431/583 (73.93%), Postives = 472/583 (80.96%), Query Frame = 0
Query: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
MSNLDEKDKQ LQ+LEDVT ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
Query: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
IP+VSYEHLKPYIDRI +GDDSPILCAHPIT+F
Sbjct: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITDF--------------------------- 120
Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
A+ ES+TK GIVARS STS +S NN++ SY
Sbjct: 121 -------------------------ARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180
Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240
Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
TGT+N KI D VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300
Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
SMS YIPQLNYY LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360
Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420
Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
KNV+LSLD EKTDEAELHNAV G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480
Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER QG
Sbjct: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 530
Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT H
Sbjct: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 530
BLAST of MC02g_new0098 vs. NCBI nr
Match:
KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 804 bits (2077), Expect = 2.15e-287
Identity = 396/581 (68.16%), Postives = 467/581 (80.38%), Query Frame = 0
Query: 5 DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
DE+DKQALQ +EDVT A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP E+EF R+LS +Y+
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
PRMKQLFP + GK M F FAKVE T++GIVA + +TS K +LNN TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189
Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
P DI+ CTD QSLYCQLLCGLCQN V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249
Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
T+N KI D VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309
Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
+ Y+P+LNYY LP+++ Y SECF G+NLDPLC P+ SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369
Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
S E+ +E VDLVDVKLG EYE+V+TTYAGLYRYC+GDI+RVTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRKN 429
Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
V+LSLD+EKTDEAELH+AV + LLS FGA + DYTS+ADSS PGHYVLYWEI K
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489
Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
ISI PSSIYEDCC AIEE L+ YR RSH+K INPLEIKIV+ TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549
Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
+SQYKTPRCV ST IIE LDS VV YFSK+ PK D H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALH 582
BLAST of MC02g_new0098 vs. NCBI nr
Match:
XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])
HSP 1 Score: 798 bits (2061), Expect = 5.61e-285
Identity = 395/581 (67.99%), Postives = 466/581 (80.21%), Query Frame = 0
Query: 5 DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
DE+DKQALQ +EDVT A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP E+EF R+LS +Y+
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
PRMKQLFP + GK M F FAKVE T++GIVA + +TS K +LNN TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189
Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
P DI+ CTD QSLYCQLLCGLCQN V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249
Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
T+N KI D VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309
Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
+ Y+P+LNYY LP+++ Y +SECF G+NLDPLC P+ SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369
Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
S E+ +E VDLVDVKLG EYE+V+TTYAGLYRY +GDI+ VTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKN 429
Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
V+LSLD+EKTDEAELH+AV + LLS FGA + DYTS+ADSS PGHYVLYWEI K
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489
Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
ISI PSSIYEDCC AIEE L+ YR RSH+K INPLEIKIV+ TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549
Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
+SQYKTPRCV ST IIE LDS VV YFSKK PK D H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALH 582
BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match:
A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)
HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0
Query: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL
Sbjct: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
Query: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI
Sbjct: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match:
A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)
HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 433/574 (75.44%), Postives = 496/574 (86.41%), Query Frame = 0
Query: 4 LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
LD KDKQ L +ED T ADEIQRRV+TEILS NAHVEYL+Q GL+GSTD STFK LIP+
Sbjct: 6 LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65
Query: 64 VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
VSYE LKPYIDRI +GDDSPILCA IT+F STGTSGG QKLIP + E R+L+ + Y
Sbjct: 66 VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125
Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183
P MKQLFPGL NGKEMFFNF + E+ TK G+VA+ +S+ K ++V+V +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185
Query: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243
DI+ CTDVYQSLYCQLLCGLCQNQ V+RVG+LFASTLIYIFKFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245
Query: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303
++AKI D VR+SVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ VVTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305
Query: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363
YIPQLN+YCK P+++VFYASSECFFGINLDPLCKPEEISYTF+PT+AYFEFLPVDR N
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365
Query: 364 -DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423
++GEVGQE VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNK PNF F RKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425
Query: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483
VILSLD+EKTDE +LH+AV + LLS GAI+VDYTSYADSSTFPGHYVLYWEIQ +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485
Query: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543
S IPS++YEDCCLA+EESLSSVYR+ARS+ KVINPLEIKIVE TF+KLME+ VD+GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545
Query: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPK 576
ISQYKTPRC+KSTQ+IE LDSKVV+NYFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578
BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match:
A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)
HSP 1 Score: 856 bits (2212), Expect = 6.00e-309
Identity = 431/583 (73.93%), Postives = 472/583 (80.96%), Query Frame = 0
Query: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
MSNLDEKDKQ LQ+LEDVT ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
Query: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
IP+VSYEHLKPYIDRI +GDDSPILCAHPIT+F
Sbjct: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITDF--------------------------- 120
Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
A+ ES+TK GIVARS STS +S NN++ SY
Sbjct: 121 -------------------------ARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180
Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240
Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
TGT+N KI D VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300
Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
SMS YIPQLNYY LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360
Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420
Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
KNV+LSLD EKTDEAELHNAV G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480
Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER QG
Sbjct: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 530
Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT H
Sbjct: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 530
BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match:
A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)
HSP 1 Score: 798 bits (2061), Expect = 2.72e-285
Identity = 395/581 (67.99%), Postives = 466/581 (80.21%), Query Frame = 0
Query: 5 DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
DE+DKQALQ +EDVT A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP E+EF R+LS +Y+
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
PRMKQLFP + GK M F FAKVE T++GIVA + +TS K +LNN TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189
Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
P DI+ CTD QSLYCQLLCGLCQN V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249
Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
T+N KI D VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309
Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
+ Y+P+LNYY LP+++ Y +SECF G+NLDPLC P+ SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369
Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
S E+ +E VDLVDVKLG EYE+V+TTYAGLYRY +GDI+ VTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKN 429
Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
V+LSLD+EKTDEAELH+AV + LLS FGA + DYTS+ADSS PGHYVLYWEI K
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489
Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
ISI PSSIYEDCC AIEE L+ YR RSH+K INPLEIKIV+ TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549
Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
+SQYKTPRCV ST IIE LDS VV YFSKK PK D H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALH 582
BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match:
A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)
HSP 1 Score: 759 bits (1961), Expect = 5.32e-270
Identity = 374/581 (64.37%), Postives = 458/581 (78.83%), Query Frame = 0
Query: 4 LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
LDEKDK+ALQ +ED+T ADEIQR++L EILS NA+VEYLQ+HGL+ STD+STFK LIP+
Sbjct: 9 LDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKLIPL 68
Query: 64 VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
V Y+ L+PYI RI +GDDS ILC++PITEF+ S+GTSG E KLIP EQEF R+LS +Y
Sbjct: 69 VYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSFFSY 128
Query: 124 MRPRMKQLFPGL--HNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYT 183
+ PRMKQLFP + H K + F+FAK E TK GIV RS+ T+ K + T
Sbjct: 129 ITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSGNNT 188
Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
SP DI+ CTD YQSLYCQLLCGL QN V RV +LFASTLI++FKFLENHW+ LA DIRT
Sbjct: 189 SPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATDIRT 248
Query: 244 GTINAKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 303
TIN KI S VR+SVM I+ KP+P++AD IE+EC KG WEGI+TRLWPN K+INAVVTG
Sbjct: 249 RTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAVVTG 308
Query: 304 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 363
SMS YIP LNYY +LP+++ Y SSECF G+NLDPLC P+E+SYT IPT+AYFEFLP+D
Sbjct: 309 SMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFLPID 368
Query: 364 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 423
N GEV Q VDLVDVKLG EYE+V+TT+AGLYRYC+GD+VRVTGFKNKAPNF FV R
Sbjct: 369 MLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSFVRR 428
Query: 424 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQF- 483
KNV+L++ EKTDEA LH AV + G ++ FGA +VDYT+YAD ST PGHYVLYWE+
Sbjct: 429 KNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWELTLD 488
Query: 484 ---KEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERM 543
++ ++ IP S++EDCCLAIE SL+ +YR ARSH+K INPLEIKIV+A TF+KLM+
Sbjct: 489 DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLMQLA 548
Query: 544 VDQGASISQYKTPRCVKSTQI--IEFLDSKVVANYFSKKYP 575
+++GASI+QYKTPRC+ S+QI I+ L+S +V+NYFSK YP
Sbjct: 549 LNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589
BLAST of MC02g_new0098 vs. TAIR 10
Match:
AT5G54510.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 698.0 bits (1800), Expect = 6.7e-201
Identity = 343/590 (58.14%), Postives = 438/590 (74.24%), Query Frame = 0
Query: 3 NLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIP 62
+L EK+K LQ +EDVT AD++QRRVL EILS NA VEYL++HGL G TD TFK+++P
Sbjct: 16 SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75
Query: 63 IVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISN 122
+V+YE ++P I+RI NGD S +LC++PI+EF TS+GTSGGE+KL+PTIE+E R+ + +
Sbjct: 76 VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135
Query: 123 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSY 182
+ P M Q PGL GK M+F F K ES T G+ AR V TS+ KS H N + ++Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195
Query: 183 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 242
TSP + C+D YQS+Y Q+LCGLCQ+++V+RVG++FAS I KFLE HW LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255
Query: 243 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 302
TGT++++I DS VR++V ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ +VTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315
Query: 303 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 362
+MS YIP L+YY LPL+ YASSEC+FG+NL PLCKP E+SYT IP +AYFEFLPV
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375
Query: 363 RTND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFK 422
R + + + QE VDLVDVKLGQEYE+VVTTYAGLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435
Query: 423 NKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPG 482
N AP F F+ RKNV+LS+D +KTDE EL NAV + L F A L +YTSYAD+S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495
Query: 483 HYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTF 542
HYVL+WE+ + IP S++EDCCL IEESL+SVYR+ R K I PLEIK+VE+ TF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555
Query: 543 DKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
DKLM+ + GASI+QYKTPRCVK IIE L+S+VV +YFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604
BLAST of MC02g_new0098 vs. TAIR 10
Match:
AT4G27260.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 694.9 bits (1792), Expect = 5.7e-200
Identity = 340/589 (57.72%), Postives = 438/589 (74.36%), Query Frame = 0
Query: 4 LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
LD+K+KQ LQ +E++T AD++QR+VL EIL+ NA VEYL++H LNG TD TFKN++P+
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76
Query: 64 VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
++YE ++P I+RI NGD SPIL + PI+EF TS+GTSGGE+KL+PTIE+E R+ + +
Sbjct: 77 ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136
Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYT 183
+ P M Q PGL NGK M+F F K ES T G+ AR V TS+ KS H + ++YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196
Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
SP + + C+D YQS+Y Q+LCGLCQ+Q+V+RVG++FAS I KFLE HW+ L DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256
Query: 244 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 303
GT+++ I D VR++V ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ +VTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316
Query: 304 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 363
MS YIP L+YY LPL+ YASSEC+FG+NL PLCKP E+SYT IP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376
Query: 364 TND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKN 423
N + + QE VDLVDVKLGQEYE+VVTTYAGL RY +GD++RVTGFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436
Query: 424 KAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGH 483
KAP F F+ RKNV+LS+D +KTDE EL NAV + L F A L +YTSYAD+S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496
Query: 484 YVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFD 543
YVL+WE+ + + IP S++EDCCLA+EES ++VYR+ R K I PLEIKIVE TFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556
Query: 544 KLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
KLM+ + GASI+QYKTPRCVK IIE L+S+VV +YFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604
BLAST of MC02g_new0098 vs. TAIR 10
Match:
AT1G59500.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 607.4 bits (1565), Expect = 1.2e-173
Identity = 286/580 (49.31%), Postives = 407/580 (70.17%), Query Frame = 0
Query: 6 EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
E + +AL+ +E++TR D +Q +VL EILS N++ EYL++ LNG+ D +FK+ +P+V
Sbjct: 18 ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77
Query: 66 YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
YE LK I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++ R+ + N +
Sbjct: 78 YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137
Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSPTD 185
P M PGL GK ++F F K ES T G+ AR TS+ KS + +S YTSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197
Query: 186 ILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTIN 245
+ C D QS+Y Q+LCGL +V R+G++F S L+ FL+N+W L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257
Query: 246 AKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSH 305
+KI D ++ + NIL KPD +LA+F+ CS+ WEGI+T++WPNTK+++ +VTG+M+
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317
Query: 306 YIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTND 365
YIP L YY LP+ ++ YASSE +FGINL+P+CKP E+SYT P +AYFEFLP + D
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377
Query: 366 SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVI 425
G V+L DV++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+ R+NV+
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437
Query: 426 LSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEIS 485
LS++ +KTDEA+L AV LL+ G +++YTSYAD+ T PGHYV+YWE+ +++ +
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497
Query: 486 IIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 545
+PS + CCL +EESL++VYR++R K I PLEI++V+ TF++LM+ + +G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557
Query: 546 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 584
+QYK PRCV T I++ LDS+VV+ +FS P W P R H
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597
BLAST of MC02g_new0098 vs. TAIR 10
Match:
AT4G37390.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 595.1 bits (1533), Expect = 6.1e-170
Identity = 287/584 (49.14%), Postives = 405/584 (69.35%), Query Frame = 0
Query: 6 EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
EKD +AL+ +E++TR D +Q +VL EIL+ N++ EYL++ L+G D TFK+ +P+V+
Sbjct: 18 EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77
Query: 66 YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
YE LKP I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++ R+ + + +
Sbjct: 78 YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137
Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
P M PGL GK ++F F K ES T G+ AR V TS+ KS H + + YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197
Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
+ + C+D QS+Y Q+LCGL +V+R+G++FAS L+ FL+N+W LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257
Query: 246 INAKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 305
++++I D ++ + IL KPD +LA+F+ CS+ WEGI+T++WPNTK+++ +VTG+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317
Query: 306 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 365
+ YIP L YY LP+ YASSE +FGINL P+CKP E+SYT +P +AYFEFLP +
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377
Query: 366 NDSGEVGQ----EFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFV 425
D V+L +V++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+
Sbjct: 378 GDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 437
Query: 426 GRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQ 485
RKNV+LS++ +KTDEAEL AV L + G +++YTSYA++ T PGHYV+YWE+
Sbjct: 438 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 497
Query: 486 FKEEISIIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMV 545
+++ + + S + CCL +EESL+SVYR++R K I PLEI++V TF++LM+ +
Sbjct: 498 GRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAI 557
Query: 546 DQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
+GASI+QYK PRCV T I+E LDS+VV+ +FS P W P R
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPER 601
BLAST of MC02g_new0098 vs. TAIR 10
Match:
AT2G14960.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 594.0 bits (1530), Expect = 1.4e-169
Identity = 290/579 (50.09%), Postives = 397/579 (68.57%), Query Frame = 0
Query: 6 EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
EKD +AL+ +E++TR AD +Q +L EIL+ NA EYL++ L G+TD TFK IP+++
Sbjct: 17 EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76
Query: 66 YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
YE L+P I RI +GD SPIL AHPI+EF TS+GTS GE+KL+PTI++E R+ + + +
Sbjct: 77 YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
P M PGL GK M+F F K E+ T G+ AR V TS+ KS H + + + YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196
Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
+ + C D +QS+Y Q+LCGL V+RVG++FAS L+ +FL+ HW A+DI G
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256
Query: 246 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 305
++++I D +R+ + ILKPDP LA+FI EC WE I+TR+WPNTK+++ +VTG+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316
Query: 306 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 365
YIP L YY LP+ YASSEC+FG+NL+P+ KP E+SYT +P +AYFEF+P+
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG--- 376
Query: 366 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 425
G + V+LVDV +G+EYE+VVTTYAGL RY +GDI+RVTGF N AP F FV RKNV
Sbjct: 377 -----GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436
Query: 426 ILSLDVEKTDEAELHNAVNKVGSLL-SSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 485
+LS+D +KTDE+EL AV S+L G+ + +YTSYAD+ST PGHYVLYWE+ ++
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496
Query: 486 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 545
CCL +EESL+SVYR++R + PLEI++V TF++LM+ + +GAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556
Query: 546 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
I+QYK PRCV T I+E LDS+VV+ +FS P W P R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 587
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9LSQ4 | 9.5e-200 | 58.14 | Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... | [more] |
| O81829 | 8.0e-199 | 57.72 | Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... | [more] |
| Q8LQM5 | 1.6e-175 | 52.44 | Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... | [more] |
| Q9LQ68 | 1.7e-172 | 49.31 | Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=G... | [more] |
| A3BLS0 | 8.6e-169 | 49.05 | Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022140778.1 | 0.0 | 100.00 | indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia] | [more] |
| XP_022140368.1 | 0.0 | 75.44 | indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia] | [more] |
| XP_022140614.1 | 1.24e-308 | 73.93 | indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia] | [more] |
| KAG6570863.1 | 2.15e-287 | 68.16 | Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... | [more] |
| XP_022944596.1 | 5.61e-285 | 67.99 | indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CIS9 | 0.0 | 100.00 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1CHU9 | 0.0 | 75.44 | indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1CG61 | 6.00e-309 | 73.93 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1FUU5 | 2.72e-285 | 67.99 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... | [more] |
| A0A5A7V3L5 | 5.32e-270 | 64.37 | Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=... | [more] |
