MC02g_new0098 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 9.5e-200
Identity = 343/590 (58.14%), Postives = 438/590 (74.24%), Query Frame = 0

Query: 3   NLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIP 62
           +L EK+K  LQ +EDVT  AD++QRRVL EILS NA VEYL++HGL G TD  TFK+++P
Sbjct: 16  SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75

Query: 63  IVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISN 122
           +V+YE ++P I+RI NGD S +LC++PI+EF TS+GTSGGE+KL+PTIE+E  R+  + +
Sbjct: 76  VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135

Query: 123 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSY 182
            + P M Q  PGL  GK M+F F K ES T  G+ AR V TS+ KS H  N   +  ++Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195

Query: 183 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 242
           TSP   + C+D YQS+Y Q+LCGLCQ+++V+RVG++FAS  I   KFLE HW  LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255

Query: 243 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 302
           TGT++++I DS VR++V  ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ +VTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315

Query: 303 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 362
           +MS YIP L+YY   LPL+   YASSEC+FG+NL PLCKP E+SYT IP +AYFEFLPV 
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375

Query: 363 RTND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFK 422
           R +            + +  QE VDLVDVKLGQEYE+VVTTYAGLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435

Query: 423 NKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPG 482
           N AP F F+ RKNV+LS+D +KTDE EL NAV    + L  F A L +YTSYAD+S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495

Query: 483 HYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTF 542
           HYVL+WE+      + IP S++EDCCL IEESL+SVYR+ R   K I PLEIK+VE+ TF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555

Query: 543 DKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
           DKLM+  +  GASI+QYKTPRCVK   IIE L+S+VV +YFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 8.0e-199
Identity = 340/589 (57.72%), Postives = 438/589 (74.36%), Query Frame = 0

Query: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
           LD+K+KQ LQ +E++T  AD++QR+VL EIL+ NA VEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
           ++YE ++P I+RI NGD SPIL + PI+EF TS+GTSGGE+KL+PTIE+E  R+  + + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYT 183
           + P M Q  PGL NGK M+F F K ES T  G+ AR V TS+ KS H      +  ++YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
           SP + + C+D YQS+Y Q+LCGLCQ+Q+V+RVG++FAS  I   KFLE HW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 244 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 303
           GT+++ I D  VR++V  ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ +VTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 304 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 363
           MS YIP L+YY   LPL+   YASSEC+FG+NL PLCKP E+SYT IP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 364 TND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKN 423
            N            + +  QE VDLVDVKLGQEYE+VVTTYAGL RY +GD++RVTGFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 424 KAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGH 483
           KAP F F+ RKNV+LS+D +KTDE EL NAV    + L  F A L +YTSYAD+S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 484 YVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFD 543
           YVL+WE+   +  + IP S++EDCCLA+EES ++VYR+ R   K I PLEIKIVE  TFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 544 KLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
           KLM+  +  GASI+QYKTPRCVK   IIE L+S+VV +YFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.6e-175
Identity = 301/574 (52.44%), Postives = 407/574 (70.91%), Query Frame = 0

Query: 10  QALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTD-ASTFKNLIPIVSYEH 69
           +AL+ +E VT  A ++QRRVL EIL+ NA  EYL+++G+ GS D    F+ L+P+V+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 70  LKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMRPRM 129
           L+P I RI NGD SPI    PI+EF TS+GTSGGE+KL+PTI  E  R+  + + + P M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 130 KQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSPTDI 189
            Q   GL  GK M+  F K ES T  G+ AR V TS+ +S    +   +  +SYTSP + 
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201

Query: 190 LCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTINA 249
           + C D YQS+Y QLLCGL     V+RVG++FAS  +    FLE HW  L +DIRTG ++ 
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261

Query: 250 KIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSHYI 309
           +I D  VR +V  +L+ DP LAD IE EC++ +WEGI+ RLWP TK+I+ +VTG+MS YI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321

Query: 310 PQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTNDSG 369
           P L +Y   LPL    YASSEC+FG+NL+P+CKP +++YT IPT+ Y+EFLPV+  N + 
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381

Query: 370 EVG-QEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVIL 429
           E   ++ VDLVDVKLG EYE+VVTTY+GLYRY +GD++RV GFKNKAP F FV R+NV L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441

Query: 430 SLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEISI 489
           S+D +KTDE ELH AV+     L+ FGA LV+YTSYAD++T PGHYVL+WE+  +   + 
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501

Query: 490 IPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASISQ 549
           +P+S++E+CCL++EE+L+SVYR+ R+  + I PLEI++V   TFDKLM+  + +GASI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561

Query: 550 YKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
           YK PRCV+   ++E LD++V   YFS K PKW P
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 593

BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.7e-172
Identity = 286/580 (49.31%), Postives = 407/580 (70.17%), Query Frame = 0

Query: 6   EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
           E + +AL+ +E++TR  D +Q +VL EILS N++ EYL++  LNG+ D  +FK+ +P+V 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 66  YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
           YE LK  I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++  R+  + N + 
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSPTD 185
           P M    PGL  GK ++F F K ES T  G+ AR   TS+ KS +      +S YTSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 186 ILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTIN 245
            + C D  QS+Y Q+LCGL    +V R+G++F S L+    FL+N+W  L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 246 AKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSH 305
           +KI D  ++  + NIL KPD +LA+F+   CS+  WEGI+T++WPNTK+++ +VTG+M+ 
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 306 YIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTND 365
           YIP L YY   LP+ ++ YASSE +FGINL+P+CKP E+SYT  P +AYFEFLP +   D
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 366 SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVI 425
            G      V+L DV++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+ R+NV+
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 426 LSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEIS 485
           LS++ +KTDEA+L  AV     LL+  G  +++YTSYAD+ T PGHYV+YWE+  +++ +
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 486 IIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 545
            +PS  +   CCL +EESL++VYR++R   K I PLEI++V+  TF++LM+  + +G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 546 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 584
           +QYK PRCV  T I++ LDS+VV+ +FS   P W P R H
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

BLAST of MC02g_new0098 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 595.1 bits (1533), Expect = 8.6e-169
Identity = 284/579 (49.05%), Postives = 398/579 (68.74%), Query Frame = 0

Query: 6   EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
           E D + L+ ++++T   D +Q RVL EIL  NA  EYL + GL+G+TD + F+  +P+VS
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 66  YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
           Y+ L+PYI RI NGD SPIL  HP++EF TS+GTS GE+KL+PTI  E  R+  + + + 
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
           P M    PGL  GK ++F F K E+ T  G+ AR V TS+ KS H  N   +   +YTSP
Sbjct: 144 PVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSP 203

Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
           T  + C D +QS+Y Q++CGLCQ   V+R+G++FAS L+   +FL+ +W  LA+DI +G 
Sbjct: 204 TAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGE 263

Query: 246 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 305
           +  ++ D  VR++V  IL PDP+LA  I +ECSKG W GI+TR+WPNTK+++ +VTG+M+
Sbjct: 264 LTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMA 323

Query: 306 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 365
            YIP L +Y   LP+    YASSEC+FG+NL P+C P E+SYT +P + YFEFLPVD T 
Sbjct: 324 QYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG 383

Query: 366 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 425
            +     + VDL  V++G+EYE+V+TTYAGL RY +GD++RVTGF N AP FRFV RKNV
Sbjct: 384 AASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNV 443

Query: 426 ILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK-EE 485
           +LS++ +KTDEAEL  AV +  +LL   GA +V+YTS A +   PGHYV+YWE+  K   
Sbjct: 444 LLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAG 503

Query: 486 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 545
            +++ +     CCL +EE+L++VYR++R     I PLEI++V   TF++LM+  + +GAS
Sbjct: 504 ATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGAS 563

Query: 546 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
           I+QYK PRCV    I+E LDS+VV+++FS   P W P R
Sbjct: 564 INQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPAR 602

BLAST of MC02g_new0098 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
           MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL
Sbjct: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60

Query: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
           IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI
Sbjct: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120

Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
           SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180

Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
           TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240

Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
           TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300

Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
           SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360

Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
           RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420

Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
           KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480

Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
           EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540

Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
           ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597

BLAST of MC02g_new0098 vs. NCBI nr
Match: XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 433/574 (75.44%), Postives = 496/574 (86.41%), Query Frame = 0

Query: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
           LD KDKQ L  +ED T  ADEIQRRV+TEILS NAHVEYL+Q GL+GSTD STFK LIP+
Sbjct: 6   LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65

Query: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
           VSYE LKPYIDRI +GDDSPILCA  IT+F  STGTSGG QKLIP  + E  R+L+ + Y
Sbjct: 66  VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125

Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183
             P MKQLFPGL NGKEMFFNF + E+ TK G+VA+ +S+   K    ++V+V  +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185

Query: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243
            DI+ CTDVYQSLYCQLLCGLCQNQ V+RVG+LFASTLIYIFKFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245

Query: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303
           ++AKI D  VR+SVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ VVTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305

Query: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363
            YIPQLN+YCK  P+++VFYASSECFFGINLDPLCKPEEISYTF+PT+AYFEFLPVDR N
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365

Query: 364 -DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423
            ++GEVGQE VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNK PNF F  RKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425

Query: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483
           VILSLD+EKTDE +LH+AV +   LLS  GAI+VDYTSYADSSTFPGHYVLYWEIQ +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485

Query: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543
            S IPS++YEDCCLA+EESLSSVYR+ARS+ KVINPLEIKIVE  TF+KLME+ VD+GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545

Query: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPK 576
           ISQYKTPRC+KSTQ+IE LDSKVV+NYFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of MC02g_new0098 vs. NCBI nr
Match: XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 856 bits (2212), Expect = 1.24e-308
Identity = 431/583 (73.93%), Postives = 472/583 (80.96%), Query Frame = 0

Query: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
           MSNLDEKDKQ LQ+LEDVT  ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1   MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60

Query: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
           IP+VSYEHLKPYIDRI +GDDSPILCAHPIT+F                           
Sbjct: 61  IPLVSYEHLKPYIDRISSGDDSPILCAHPITDF--------------------------- 120

Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
                                    A+ ES+TK GIVARS STS  +S   NN++   SY
Sbjct: 121 -------------------------ARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180

Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
           TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240

Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
           TGT+N KI D  VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300

Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
           SMS YIPQLNYY   LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360

Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
           R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420

Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
           KNV+LSLD EKTDEAELHNAV   G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480

Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
           EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER   QG
Sbjct: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 530

Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
           ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT  H
Sbjct: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 530

BLAST of MC02g_new0098 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804 bits (2077), Expect = 2.15e-287
Identity = 396/581 (68.16%), Postives = 467/581 (80.38%), Query Frame = 0

Query: 5   DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
           DE+DKQALQ +EDVT  A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10  DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69

Query: 65  SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
           SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP  E+EF R+LS  +Y+
Sbjct: 70  SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129

Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
            PRMKQLFP   +  GK M F FAKVE  T++GIVA + +TS  K  +LNN       TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189

Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
           P DI+ CTD  QSLYCQLLCGLCQN  V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249

Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
           T+N KI D  VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309

Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
           + Y+P+LNYY   LP+++  Y  SECF G+NLDPLC P+  SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369

Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
             S E+ +E VDLVDVKLG EYE+V+TTYAGLYRYC+GDI+RVTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRKN 429

Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
           V+LSLD+EKTDEAELH+AV +   LLS FGA + DYTS+ADSS  PGHYVLYWEI  K  
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489

Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
           ISI PSSIYEDCC AIEE L+  YR  RSH+K INPLEIKIV+  TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549

Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
           +SQYKTPRCV ST IIE LDS VV  YFSK+ PK D    H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALH 582

BLAST of MC02g_new0098 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 798 bits (2061), Expect = 5.61e-285
Identity = 395/581 (67.99%), Postives = 466/581 (80.21%), Query Frame = 0

Query: 5   DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
           DE+DKQALQ +EDVT  A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10  DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69

Query: 65  SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
           SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP  E+EF R+LS  +Y+
Sbjct: 70  SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129

Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
            PRMKQLFP   +  GK M F FAKVE  T++GIVA + +TS  K  +LNN       TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189

Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
           P DI+ CTD  QSLYCQLLCGLCQN  V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249

Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
           T+N KI D  VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309

Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
           + Y+P+LNYY   LP+++  Y +SECF G+NLDPLC P+  SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369

Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
             S E+ +E VDLVDVKLG EYE+V+TTYAGLYRY +GDI+ VTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKN 429

Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
           V+LSLD+EKTDEAELH+AV +   LLS FGA + DYTS+ADSS  PGHYVLYWEI  K  
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489

Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
           ISI PSSIYEDCC AIEE L+  YR  RSH+K INPLEIKIV+  TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549

Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
           +SQYKTPRCV ST IIE LDS VV  YFSKK PK D    H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALH 582

BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 1202 bits (3111), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
           MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL
Sbjct: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60

Query: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
           IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI
Sbjct: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120

Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
           SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180

Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
           TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240

Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
           TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300

Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
           SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360

Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
           RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420

Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
           KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480

Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
           EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540

Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597
           ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPHPNLFGPASSFDEIS 597

BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match: A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 433/574 (75.44%), Postives = 496/574 (86.41%), Query Frame = 0

Query: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
           LD KDKQ L  +ED T  ADEIQRRV+TEILS NAHVEYL+Q GL+GSTD STFK LIP+
Sbjct: 6   LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65

Query: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
           VSYE LKPYIDRI +GDDSPILCA  IT+F  STGTSGG QKLIP  + E  R+L+ + Y
Sbjct: 66  VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125

Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183
             P MKQLFPGL NGKEMFFNF + E+ TK G+VA+ +S+   K    ++V+V  +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185

Query: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243
            DI+ CTDVYQSLYCQLLCGLCQNQ V+RVG+LFASTLIYIFKFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245

Query: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303
           ++AKI D  VR+SVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ VVTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305

Query: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363
            YIPQLN+YCK  P+++VFYASSECFFGINLDPLCKPEEISYTF+PT+AYFEFLPVDR N
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365

Query: 364 -DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423
            ++GEVGQE VDLVDVKLGQEYE+VVTTYAGLYRYC+GDI+RVTGFKNK PNF F  RKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425

Query: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483
           VILSLD+EKTDE +LH+AV +   LLS  GAI+VDYTSYADSSTFPGHYVLYWEIQ +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485

Query: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543
            S IPS++YEDCCLA+EESLSSVYR+ARS+ KVINPLEIKIVE  TF+KLME+ VD+GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545

Query: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPK 576
           ISQYKTPRC+KSTQ+IE LDSKVV+NYFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578

BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match: A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)

HSP 1 Score: 856 bits (2212), Expect = 6.00e-309
Identity = 431/583 (73.93%), Postives = 472/583 (80.96%), Query Frame = 0

Query: 1   MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
           MSNLDEKDKQ LQ+LEDVT  ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1   MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60

Query: 61  IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
           IP+VSYEHLKPYIDRI +GDDSPILCAHPIT+F                           
Sbjct: 61  IPLVSYEHLKPYIDRISSGDDSPILCAHPITDF--------------------------- 120

Query: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
                                    A+ ES+TK GIVARS STS  +S   NN++   SY
Sbjct: 121 -------------------------ARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180

Query: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
           TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240

Query: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
           TGT+N KI D  VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300

Query: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
           SMS YIPQLNYY   LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360

Query: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
           R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420

Query: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
           KNV+LSLD EKTDEAELHNAV   G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480

Query: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
           EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER   QG
Sbjct: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 530

Query: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
           ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT  H
Sbjct: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 530

BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 2.72e-285
Identity = 395/581 (67.99%), Postives = 466/581 (80.21%), Query Frame = 0

Query: 5   DEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIV 64
           DE+DKQALQ +EDVT  A +IQR++LT+ILSTNA VEYLQQHGL+GST+ STFKNLIP++
Sbjct: 10  DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69

Query: 65  SYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYM 124
           SYE L+PYIDRI NGDDSPILC +PITEF+ S+GTSGGE KLIP  E+EF R+LS  +Y+
Sbjct: 70  SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129

Query: 125 RPRMKQLFPG--LHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 184
            PRMKQLFP   +  GK M F FAKVE  T++GIVA + +TS  K  +LNN       TS
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN-------TS 189

Query: 185 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 244
           P DI+ CTD  QSLYCQLLCGLCQN  V RVG+LFASTLI++ KFLE+HWV+LA+DIR G
Sbjct: 190 PKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAG 249

Query: 245 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 304
           T+N KI D  VR++VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INA+VTGSM
Sbjct: 250 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSM 309

Query: 305 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 364
           + Y+P+LNYY   LP+++  Y +SECF G+NLDPLC P+  SYT IPT+AYFEFLP+DRT
Sbjct: 310 AQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRT 369

Query: 365 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 424
             S E+ +E VDLVDVKLG EYE+V+TTYAGLYRY +GDI+ VTGFKNKAPNF FV RKN
Sbjct: 370 KSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKN 429

Query: 425 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 484
           V+LSLD+EKTDEAELH+AV +   LLS FGA + DYTS+ADSS  PGHYVLYWEI  K  
Sbjct: 430 VVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGG 489

Query: 485 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 544
           ISI PSSIYEDCC AIEE L+  YR  RSH+K INPLEIKIV+  TF+KLM + +++GAS
Sbjct: 490 ISI-PSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 549

Query: 545 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
           +SQYKTPRCV ST IIE LDS VV  YFSKK PK D    H
Sbjct: 550 MSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALH 582

BLAST of MC02g_new0098 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 759 bits (1961), Expect = 5.32e-270
Identity = 374/581 (64.37%), Postives = 458/581 (78.83%), Query Frame = 0

Query: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
           LDEKDK+ALQ +ED+T  ADEIQR++L EILS NA+VEYLQ+HGL+ STD+STFK LIP+
Sbjct: 9   LDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKLIPL 68

Query: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
           V Y+ L+PYI RI +GDDS ILC++PITEF+ S+GTSG E KLIP  EQEF R+LS  +Y
Sbjct: 69  VYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSFFSY 128

Query: 124 MRPRMKQLFPGL--HNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYT 183
           + PRMKQLFP +  H  K + F+FAK E  TK GIV RS+ T+  K           + T
Sbjct: 129 ITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSGNNT 188

Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
           SP DI+ CTD YQSLYCQLLCGL QN  V RV +LFASTLI++FKFLENHW+ LA DIRT
Sbjct: 189 SPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATDIRT 248

Query: 244 GTINAKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 303
            TIN KI  S VR+SVM I+ KP+P++AD IE+EC KG WEGI+TRLWPN K+INAVVTG
Sbjct: 249 RTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAVVTG 308

Query: 304 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 363
           SMS YIP LNYY  +LP+++  Y SSECF G+NLDPLC P+E+SYT IPT+AYFEFLP+D
Sbjct: 309 SMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFLPID 368

Query: 364 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 423
             N  GEV Q  VDLVDVKLG EYE+V+TT+AGLYRYC+GD+VRVTGFKNKAPNF FV R
Sbjct: 369 MLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSFVRR 428

Query: 424 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQF- 483
           KNV+L++  EKTDEA LH AV + G ++  FGA +VDYT+YAD ST PGHYVLYWE+   
Sbjct: 429 KNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWELTLD 488

Query: 484 ---KEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERM 543
              ++ ++ IP S++EDCCLAIE SL+ +YR ARSH+K INPLEIKIV+A TF+KLM+  
Sbjct: 489 DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLMQLA 548

Query: 544 VDQGASISQYKTPRCVKSTQI--IEFLDSKVVANYFSKKYP 575
           +++GASI+QYKTPRC+ S+QI  I+ L+S +V+NYFSK YP
Sbjct: 549 LNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589

BLAST of MC02g_new0098 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 698.0 bits (1800), Expect = 6.7e-201
Identity = 343/590 (58.14%), Postives = 438/590 (74.24%), Query Frame = 0

Query: 3   NLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIP 62
           +L EK+K  LQ +EDVT  AD++QRRVL EILS NA VEYL++HGL G TD  TFK+++P
Sbjct: 16  SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75

Query: 63  IVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISN 122
           +V+YE ++P I+RI NGD S +LC++PI+EF TS+GTSGGE+KL+PTIE+E  R+  + +
Sbjct: 76  VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135

Query: 123 YMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSY 182
            + P M Q  PGL  GK M+F F K ES T  G+ AR V TS+ KS H  N   +  ++Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195

Query: 183 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 242
           TSP   + C+D YQS+Y Q+LCGLCQ+++V+RVG++FAS  I   KFLE HW  LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255

Query: 243 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 302
           TGT++++I DS VR++V  ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ +VTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315

Query: 303 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 362
           +MS YIP L+YY   LPL+   YASSEC+FG+NL PLCKP E+SYT IP +AYFEFLPV 
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375

Query: 363 RTND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFK 422
           R +            + +  QE VDLVDVKLGQEYE+VVTTYAGLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435

Query: 423 NKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPG 482
           N AP F F+ RKNV+LS+D +KTDE EL NAV    + L  F A L +YTSYAD+S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495

Query: 483 HYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTF 542
           HYVL+WE+      + IP S++EDCCL IEESL+SVYR+ R   K I PLEIK+VE+ TF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555

Query: 543 DKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
           DKLM+  +  GASI+QYKTPRCVK   IIE L+S+VV +YFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

BLAST of MC02g_new0098 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 694.9 bits (1792), Expect = 5.7e-200
Identity = 340/589 (57.72%), Postives = 438/589 (74.36%), Query Frame = 0

Query: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63
           LD+K+KQ LQ +E++T  AD++QR+VL EIL+ NA VEYL++H LNG TD  TFKN++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123
           ++YE ++P I+RI NGD SPIL + PI+EF TS+GTSGGE+KL+PTIE+E  R+  + + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYT 183
           + P M Q  PGL NGK M+F F K ES T  G+ AR V TS+ KS H      +  ++YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 184 SPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRT 243
           SP + + C+D YQS+Y Q+LCGLCQ+Q+V+RVG++FAS  I   KFLE HW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 244 GTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGS 303
           GT+++ I D  VR++V  ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ +VTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 304 MSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDR 363
           MS YIP L+YY   LPL+   YASSEC+FG+NL PLCKP E+SYT IP++AYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 364 TND-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKN 423
            N            + +  QE VDLVDVKLGQEYE+VVTTYAGL RY +GD++RVTGFKN
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436

Query: 424 KAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGH 483
           KAP F F+ RKNV+LS+D +KTDE EL NAV    + L  F A L +YTSYAD+S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496

Query: 484 YVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFD 543
           YVL+WE+   +  + IP S++EDCCLA+EES ++VYR+ R   K I PLEIKIVE  TFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 544 KLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDP 580
           KLM+  +  GASI+QYKTPRCVK   IIE L+S+VV +YFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

BLAST of MC02g_new0098 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 607.4 bits (1565), Expect = 1.2e-173
Identity = 286/580 (49.31%), Postives = 407/580 (70.17%), Query Frame = 0

Query: 6   EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
           E + +AL+ +E++TR  D +Q +VL EILS N++ EYL++  LNG+ D  +FK+ +P+V 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 66  YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
           YE LK  I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++  R+  + N + 
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSPTD 185
           P M    PGL  GK ++F F K ES T  G+ AR   TS+ KS +      +S YTSP +
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197

Query: 186 ILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGTIN 245
            + C D  QS+Y Q+LCGL    +V R+G++F S L+    FL+N+W  L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257

Query: 246 AKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMSH 305
           +KI D  ++  + NIL KPD +LA+F+   CS+  WEGI+T++WPNTK+++ +VTG+M+ 
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317

Query: 306 YIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTND 365
           YIP L YY   LP+ ++ YASSE +FGINL+P+CKP E+SYT  P +AYFEFLP +   D
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377

Query: 366 SGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNVI 425
            G      V+L DV++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+ R+NV+
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437

Query: 426 LSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEIS 485
           LS++ +KTDEA+L  AV     LL+  G  +++YTSYAD+ T PGHYV+YWE+  +++ +
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497

Query: 486 IIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGASI 545
            +PS  +   CCL +EESL++VYR++R   K I PLEI++V+  TF++LM+  + +G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557

Query: 546 SQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 584
           +QYK PRCV  T I++ LDS+VV+ +FS   P W P R H
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597

BLAST of MC02g_new0098 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 595.1 bits (1533), Expect = 6.1e-170
Identity = 287/584 (49.14%), Postives = 405/584 (69.35%), Query Frame = 0

Query: 6   EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
           EKD +AL+ +E++TR  D +Q +VL EIL+ N++ EYL++  L+G  D  TFK+ +P+V+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 66  YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
           YE LKP I RI NGD SPIL +HPITEF TS+GTS GE+KL+PTIE++  R+  + + + 
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
           P M    PGL  GK ++F F K ES T  G+ AR V TS+ KS H      +  + YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
            + + C+D  QS+Y Q+LCGL    +V+R+G++FAS L+    FL+N+W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 246 INAKIMDSYVRKSVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 305
           ++++I D  ++  +  IL KPD +LA+F+   CS+  WEGI+T++WPNTK+++ +VTG+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 306 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 365
           + YIP L YY   LP+    YASSE +FGINL P+CKP E+SYT +P +AYFEFLP +  
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377

Query: 366 NDSGEVGQ----EFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFV 425
            D            V+L +V++G+EYE+V+TTYAGLYRY +GDI+RVTGF N AP F+F+
Sbjct: 378 GDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 437

Query: 426 GRKNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQ 485
            RKNV+LS++ +KTDEAEL  AV     L +  G  +++YTSYA++ T PGHYV+YWE+ 
Sbjct: 438 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 486 FKEEISIIPS-SIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMV 545
            +++ + + S  +   CCL +EESL+SVYR++R   K I PLEI++V   TF++LM+  +
Sbjct: 498 GRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAI 557

Query: 546 DQGASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
            +GASI+QYK PRCV  T I+E LDS+VV+ +FS   P W P R
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPER 601

BLAST of MC02g_new0098 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 594.0 bits (1530), Expect = 1.4e-169
Identity = 290/579 (50.09%), Postives = 397/579 (68.57%), Query Frame = 0

Query: 6   EKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPIVS 65
           EKD +AL+ +E++TR AD +Q  +L EIL+ NA  EYL++  L G+TD  TFK  IP+++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 66  YEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNYMR 125
           YE L+P I RI +GD SPIL AHPI+EF TS+GTS GE+KL+PTI++E  R+  + + + 
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 126 PRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVE--SSYTSP 185
           P M    PGL  GK M+F F K E+ T  G+ AR V TS+ KS H  +   +  + YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 186 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 245
            + + C D +QS+Y Q+LCGL     V+RVG++FAS L+   +FL+ HW   A+DI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 246 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 305
           ++++I D  +R+ +  ILKPDP LA+FI  EC    WE I+TR+WPNTK+++ +VTG+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316

Query: 306 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 365
            YIP L YY   LP+    YASSEC+FG+NL+P+ KP E+SYT +P +AYFEF+P+    
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG--- 376

Query: 366 DSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKNV 425
                G + V+LVDV +G+EYE+VVTTYAGL RY +GDI+RVTGF N AP F FV RKNV
Sbjct: 377 -----GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436

Query: 426 ILSLDVEKTDEAELHNAVNKVGSLL-SSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 485
           +LS+D +KTDE+EL  AV    S+L    G+ + +YTSYAD+ST PGHYVLYWE+  ++ 
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496

Query: 486 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 545
                      CCL +EESL+SVYR++R     + PLEI++V   TF++LM+  + +GAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556

Query: 546 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTR 582
           I+QYK PRCV  T I+E LDS+VV+ +FS   P W P R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 587