MC01g1630 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1630 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 581/844 (68.84%), Postives = 695/844 (82.35%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCLDEFDDS-----TAVYIVTLKEPSAATHYYGELRKNTTSFRIGTS 68
           +L + CFG+   +   D   DS     TAVYIVTL++ S + H + +  +     R  + 
Sbjct: 11  VLLLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQAS-SLHLFQQEAEEVKRVRDQSK 70

Query: 69  GGLSIH------KPRNISRTH--RRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGF 128
            G +        +PRNISR+   R   S IA+ HDSLLR  LKGEKY+KLYS+H+LINGF
Sbjct: 71  HGDTSKFTRPKLQPRNISRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGF 130

Query: 129 AVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVI 188
           AV V+ +QA  LS+RREVAN+VLDFSVRTATT+TPQF+GLP+GAW ++GGYE+AG GIVI
Sbjct: 131 AVFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVI 190

Query: 189 GFIDTGIDPTHPSF-ADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAI 248
           GFIDTGIDPTHPSF   D +   +PIP+HFSG+CEVTPDFPSGSCNRKLVGARHFA SAI
Sbjct: 191 GFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAI 250

Query: 249 TRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKA 308
           TRGIFN+S+DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG+APR+HI+VYKA
Sbjct: 251 TRGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKA 310

Query: 309 LYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIF 368
           LYKSFGGFAADVVAA+DQAAQDGVDI+SLSITPNRRPPG+ATFFNP+DMA+LSAVKAGIF
Sbjct: 311 LYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIF 370

Query: 369 VVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTM 428
           VVQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T     
Sbjct: 371 VVQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKK 430

Query: 429 YKLVAAIHALSNGTNVSD-DMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRA 488
           Y +++A+ AL N ++V D DMYVGECQD  +FD+D + GNLLICSYSIRFVLGLST+K+A
Sbjct: 431 YTMISALDALKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQA 490

Query: 489 LQTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLT 548
           L  AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG T
Sbjct: 491 LAVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTT 550

Query: 549 KKISKFGAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIW 608
           K+I +FGAVA I+GG  AN+S  AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGNSIW
Sbjct: 551 KEIVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIW 610

Query: 609 AAWSSVATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASL 668
            AWSS AT+S EF GE+FAMMSGTSMAAPH+AG+A+L+KQKF   SPSAIASALSTT+ L
Sbjct: 611 GAWSSAATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVL 670

Query: 669 YDRTGGPIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGI 728
           +D  G  IMAQRAYANP+Q  SPATPFDMG+GFVNATAAL+PGLIFD+S++DYMSFLCGI
Sbjct: 671 FDNKGEAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGI 730

Query: 729 NGSAPVVFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGW 788
           NGSAPVVFNYTG NC   N++ISG+DLNLPS+T++KLN +R VQR +TNIAG E Y+V  
Sbjct: 731 NGSAPVVFNYTGTNCLRNNATISGSDLNLPSITVSKLNNTRTVQRLMTNIAGNETYTVSL 790

Query: 789 SAPYGISLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVI 838
             P+ + + VSPT+FSI +GE ++L++   A  NSS++SFG I L G+ GHIV IP+SV 
Sbjct: 791 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 850

BLAST of MC01g1630 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 568/836 (67.94%), Postives = 685/836 (81.94%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCL---DEFDDSTAVYIVTLKEPSAATHYY--GELRKNTTSFRIGTS 68
           LL ++ F +FL  + L    + DD +AVYIVTLK+P    H +   EL+   + F     
Sbjct: 12  LLLMISF-VFLSNNTLGQQQDDDDDSAVYIVTLKQP-PIVHLFEEQELKHKKSKFTPKL- 71

Query: 69  GGLSIHKPRNISRTHRRYSSY--IARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVTQ 128
                 +PRN SR     S    + + HDS LRK LKGEKY+KLYSYH+LINGFA+ +  
Sbjct: 72  ------RPRNNSRKRHGKSKIPSVVQSHDSFLRKTLKGEKYIKLYSYHYLINGFALFINS 131

Query: 129 EQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDTG 188
           +QA KLS R+EVAN+VLD+SVRTATT+TPQF+GLPQGAW ++GG+E AG G++IGFIDTG
Sbjct: 132 QQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTG 191

Query: 189 IDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 248
           IDP HPSF D+ +   +PIP HFSG+CEVTPDFPSGSCN+KL+GARHFA SA+TRGIFN+
Sbjct: 192 IDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNS 251

Query: 249 SQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFGG 308
           S+DYASPFDGDGHGTHTAS+AAGNHG+PV+V+ H+FG ASG+APR+ I+VYKALYKSFGG
Sbjct: 252 SEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFISVYKALYKSFGG 311

Query: 309 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGN 368
           FAADVVAA+DQAAQDGVDI+SLSITPNR+PPG+ATFFNPIDMALLSAVKAGIFVVQAAGN
Sbjct: 312 FAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALLSAVKAGIFVVQAAGN 371

Query: 369 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAAI 428
           TGPAPK+MSSFSPWIFTVGA+SHDR Y+NS++LGNNVTIPG+G A  T +  MYK+++A 
Sbjct: 372 TGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAF 431

Query: 429 HALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLS 488
           HAL+N T+V  DMYVGECQD  NFD+DRV G LLICSYS RFVLGLST+K+AL  AKNLS
Sbjct: 432 HALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQALDVAKNLS 491

Query: 489 AAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFGA 548
           A GVIFY+D +V+GF +NP PM MPGIII S EDSK LL+YYNSS++ D  TK+I  FGA
Sbjct: 492 ATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGA 551

Query: 549 VACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVAT 608
           VA I GGL AN+S  AP++MYYSARGPDPED+S +D+D++KPNLVAPGNSIW AWSS +T
Sbjct: 552 VAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSAST 611

Query: 609 DSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGPI 668
           DS EF GE FAMMSGTSMAAPH+AG+A+LIKQ +P  +PS I+SALSTTA L D  G PI
Sbjct: 612 DSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKGSPI 671

Query: 669 MAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVVF 728
           MAQR Y+NP+Q+   ATP DMGSGFVNATAAL+PGL+FD+S++DY+SFLCGINGS  VVF
Sbjct: 672 MAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSDTVVF 731

Query: 729 NYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGISL 788
           NYTG  C   N+ +SG DLNLPS+T++ L+ ++  QRS+ NIAG E Y+VGWS PYG+S+
Sbjct: 732 NYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSM 791

Query: 789 KVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKIS 838
           KVSPT+FSI  GE QVL++    T NSS +SFGRIGLFG+ GHIVNIP++VI+KI+
Sbjct: 792 KVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKIA 838

BLAST of MC01g1630 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 1.7e-312
Identity = 546/832 (65.62%), Postives = 663/832 (79.69%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRIGTSGGLSI 68
           LLC+V   +F  A        S+AVYIVTLK+   + H+ G    ++      TS     
Sbjct: 12  LLCLVSSSVFCLAESDQNATVSSAVYIVTLKD-RPSVHFSGRESSDSKHSLTATSS---- 71

Query: 69  HKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVTQEQANKLS 128
                I RT  R S+ I R+HDSLLR VL+ E YLKLYSYH+LINGF+ ++T++QA++L+
Sbjct: 72  ----QIYRTLNR-SASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLA 131

Query: 129 KRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDTGIDPTHPS 188
            R EV NVVLDF V  ATTHTPQFLGLP+GAW +DGG E AG G+VIGFIDTGIDPTHPS
Sbjct: 132 AREEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPS 191

Query: 189 FADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNASQDYASP 248
           F+D ++ + + +P HF+G+CEVT  FP GSCNRKL+GARHFA SA++RG+ N+SQD ASP
Sbjct: 192 FSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASP 251

Query: 249 FDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVA 308
           FDG+GHGTHTAS+AAGNHGIPV+VAGH  GNASGMAPR+HIA+YKALYK FGGFAAD++A
Sbjct: 252 FDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIA 311

Query: 309 AVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS 368
           A+DQAAQDGVDII+LSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS
Sbjct: 312 AIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS 371

Query: 369 MSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAAIHALSNGT 428
           MSSFSPWIFTVGA SHDR Y+NSI LGNNVTIPGVGLA GT    M+KLV A HAL NGT
Sbjct: 372 MSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT--RIMHKLVLATHALRNGT 431

Query: 429 NVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLSAAGVIFY 488
            V D +YVGECQD+S+FD+  V+G +L+CSY++RF+LG+ST+K+AL TAKNL+AAG++FY
Sbjct: 432 TVMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFY 491

Query: 489 MDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFGAVACISGG 548
           +D    GF++   PM +PGI+ISSP+DS+ LL+YYNSSL  +  + KI    +VA I GG
Sbjct: 492 IDPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGG 551

Query: 549 LKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVATDSIEFLG 608
           ++  Y  +AP++MY+SARGPDPED S  D+DI+KPNLVAPGN+IW AWS +   + +F G
Sbjct: 552 MRPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQG 611

Query: 609 ENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGPIMAQRAYA 668
           E FAM SGTSM+APH+ G+A+LIKQKFP  +P+AIASALSTTASL DR G  IMAQR   
Sbjct: 612 ERFAMESGTSMSAPHVTGIAALIKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVL 671

Query: 669 NPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVVFNYTGQNC 728
           NP+ +QSPATPFDMGSGFVNATAAL+PGLIFD  Y++YM FLCGINGS+PVV NYTG++C
Sbjct: 672 NPDISQSPATPFDMGSGFVNATAALDPGLIFDIGYNEYMKFLCGINGSSPVVLNYTGESC 731

Query: 729 RVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGP---EFYSVGWSAPYGISLKVSP 788
             YNSS++ +DLNLPSVTIAKL  +R V R VTNIA     E Y VGW AP  +S+KVSP
Sbjct: 732 SSYNSSLAASDLNLPSVTIAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSP 791

Query: 789 TRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKIS 838
            +F+IGNG+ +VL++ F A  N S+ASFGRIGLFG  GH+VNIP++VI KI+
Sbjct: 792 AKFTIGNGQTRVLSLVFRAMKNVSMASFGRIGLFGDRGHVVNIPVAVIYKIA 831

BLAST of MC01g1630 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 3.2e-185
Identity = 369/805 (45.84%), Postives = 494/805 (61.37%), Query Frame = 0

Query: 33  VYIVTLKEPSAATHYYGELRKNTTSFRIGTSGGLSIHKPRNISRTHRRYSSYIARIHDSL 92
           VYIVT++     ++  GE     T+         S  K    S     Y+ ++ R HD +
Sbjct: 21  VYIVTMEGDPIISYKGGENGFEATAVE-------SDEKIDTSSELVTVYARHLERKHDMI 80

Query: 93  LRKVLKGEKYLKLYSYHFLINGFAVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQF 152
           L  + +   Y KLYSY  LINGFA  V+ EQA  L +   V +V  D+ VR  TTHTP+F
Sbjct: 81  LGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEF 140

Query: 153 LGLPQGAWSQDGGYESAGAGIVIGFIDTGIDPTHPSFADDLTDNPFPIPSHFSGICEVTP 212
           LGLP   W   GG++ AG  IVIGF+D+GI P HPSFA        P+P H+ G CE  P
Sbjct: 141 LGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLP-HYKGKCEEDP 200

Query: 213 DFPSGSCNRKLVGARHFAASAITRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLV 272
                 CNRK+VGA+HFA +A   G FN   DYASP DGDGHG+HTA+IAAGN+GIP+ +
Sbjct: 201 HTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRM 260

Query: 273 AGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPP 332
            G+ FG ASGMAPR+ IAVYKALY+ FGGF ADVVAA+DQA  DGVDI+SLS+ PN  P 
Sbjct: 261 HGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPT 320

Query: 333 GI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANS 392
               TF NP D  LL AVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N 
Sbjct: 321 TTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNH 380

Query: 393 ISLGNNVTIPGVGLAPGTYNDTMYKLVAAIHALSNGT----NVSDDMYVGECQDASNFDR 452
           ++LGN   + G+GL+P T    +Y LV+A   L + +    N SD      CQ    F++
Sbjct: 381 LTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSD------CQRPEVFNK 440

Query: 453 DRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPG 512
             VEGN+L+C YS  FV+G +++K+ + TAK+L AAG +  +++   G + +P+P  +PG
Sbjct: 441 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 500

Query: 513 IIISSPEDSKILLQYYNSSLEVDGLTKKISKFGAVACISGGLKANYSTSAPQIMYYSARG 572
           I+I+    S  L+ YYN+S   D  T ++  F A   I  GL      SAPQ+  +SARG
Sbjct: 501 ILITDVSKSMDLIDYYNASTSRD-WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARG 560

Query: 573 PDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVATDSIEFLGENFAMMSGTSMAAPHIAGL 632
           P+ +D S  D+D++KP+++APG  IWAAW    TD   ++GE FA++SGTSMAAPHIAG+
Sbjct: 561 PNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGI 620

Query: 633 ASLIKQKFPSLSPSAIASALSTTASLYDRTGGPIMAQRAYANPEQNQSPATPFDMGSGFV 692
           A+L+KQK P  SP+AI SAL TT+++ DR G  + AQ+           ATPFD GSG V
Sbjct: 621 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHV 680

Query: 693 NATAALNPGLIFDSSYDDYMSFLCGING-SAPVVFNYTGQNCRVYNSSISGADLNLPSVT 752
           N +AAL+PGLIFD+ Y+DY+ FLC   G SA  + NYT   C  Y+     ++ N PS+ 
Sbjct: 681 NPSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACN-YDMK-HPSNFNAPSIA 740

Query: 753 IAKLNQSRVVQRSVTNIAG-PEFYSVGWSAPYGISLKVSPTRFSIGNGEKQVLTIFFNAT 812
           ++ L  ++ V R VTN+A   E Y++       I+++V+P   ++  G  +  ++     
Sbjct: 741 VSHLVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVR 800

Query: 813 MNSSVASFGRIGLFGSEGHIVNIPL 831
             S V SFG + L GS GH V IP+
Sbjct: 801 SVSGVYSFGEVKLKGSRGHKVRIPV 808

BLAST of MC01g1630 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 4.2e-185
Identity = 357/801 (44.57%), Postives = 491/801 (61.30%), Query Frame = 0

Query: 33  VYIVTLKEPSAATHYYGELRKNTTSFRIGTSGGLSIHKPRNISRTHRRYSSYIARIHDSL 92
           +YIVT++     ++  G+     T+         S  K    S     Y+ ++ R HD L
Sbjct: 21  IYIVTMEGEPIISYKGGDNGFEATAVE-------SDEKIDTTSELVTSYARHLERKHDML 80

Query: 93  LRKVLKGEKYLKLYSYHFLINGFAVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQF 152
           L  +     Y KLYSY  LINGFA  V+ +QA  L +   V +V  D+ VR  TTHTPQF
Sbjct: 81  LGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQF 140

Query: 153 LGLPQGAWSQDGGYESAGAGIVIGFIDTGIDPTHPSFADDLTDNPFPIPSHFSGICEVTP 212
           LGLP   W   GGY+ AG  IVIGFID+GI P HPSFA   T  P+     + G CE  P
Sbjct: 141 LGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDP 200

Query: 213 DFPSGSCNRKLVGARHFAASAITRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLV 272
                 CN K++GA+HFA +A   G FN   D+ASP DGDGHG+HTA+IAAGN+GIPV +
Sbjct: 201 HTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRM 260

Query: 273 AGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPP 332
            G+ FG ASGMAPR+ IAVYKALY+ FGGF ADVVAA+DQA  DGVDI+SLS+ PN  P 
Sbjct: 261 HGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPA 320

Query: 333 GI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANS 392
               TF NP D  LL AVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N 
Sbjct: 321 TTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNH 380

Query: 393 ISLGNNVTIPGVGLAPGTYNDTMYKLVAAIHALSNGTNVSDDMYVGECQDASNFDRDRVE 452
           ++LGN   + G+GL+P T     YK+V+A   L   + +  +    +CQ     ++  VE
Sbjct: 381 LTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYN--PSDCQKPEVLNKKLVE 440

Query: 453 GNLLICSYSIRFVLGLSTVKRALQTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPGIIIS 512
           GN+L+C YS  FV G +++K+  +TAK+L AAG +  +++   G + +P+P  +PGI+I+
Sbjct: 441 GNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPGILIT 500

Query: 513 SPEDSKILLQYYNSSLEVDGLTKKISKFGAVACISGGLKANYSTSAPQIMYYSARGPDPE 572
               S  L+ YYN +   D +  ++  F A   I  GL+     SAP++  +SARGP+ +
Sbjct: 501 DVSKSMDLIDYYNVTTSRDWM-GRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTK 560

Query: 573 DSSLDDSDIVKPNLVAPGNSIWAAWSSVATDSIEFLGENFAMMSGTSMAAPHIAGLASLI 632
           D S  D+D++KP+++APG+ IW+AWS+  TD   ++GE FA++SGTSMAAPHIAG+A+L+
Sbjct: 561 DFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGIAALV 620

Query: 633 KQKFPSLSPSAIASALSTTASLYDRTGGPIMAQRAYANPEQNQSPATPFDMGSGFVNATA 692
           KQK P  SP+AI SAL TT+++ DR G P+ AQ+           ATPFD GSG VN +A
Sbjct: 621 KQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSA 680

Query: 693 ALNPGLIFDSSYDDYMSFLCGING-SAPVVFNYTGQNCRVYNSSISGADLNLPSVTIAKL 752
           AL+PGLIFD+ Y+DY+ FLC   G  A  + N+T   C      +  ++ N PS+ I+ L
Sbjct: 681 ALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNF--KMVHPSNFNTPSIAISHL 740

Query: 753 NQSRVVQRSVTNIA-GPEFYSVGWSAPYGISLKVSPTRFSIGNGEKQVLTIFFNATMNSS 812
            +++ V R VTN+A   E Y++       I+++VSP   ++  G  +  ++       + 
Sbjct: 741 VRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVTG 800

Query: 813 VASFGRIGLFGSEGHIVNIPL 831
             SFG++ L GS GH V +P+
Sbjct: 801 AYSFGQVTLKGSRGHKVTLPV 809

BLAST of MC01g1630 vs. NCBI nr
Match: XP_022146600.1 (subtilisin-like protease SBT2.3 [Momordica charantia])

HSP 1 Score: 1672 bits (4331), Expect = 0.0
Identity = 843/843 (100.00%), Postives = 843/843 (100.00%), Query Frame = 0

Query: 1   MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI 60
           MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI
Sbjct: 1   MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI 60

Query: 61  GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT 120
           GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT
Sbjct: 61  GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT 120

Query: 121 QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT 180
           QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT
Sbjct: 121 QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT 180

Query: 181 GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN 240
           GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN
Sbjct: 181 GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN 240

Query: 241 ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG 300
           ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG
Sbjct: 241 ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG 300

Query: 301 GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG 360
           GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG
Sbjct: 301 GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG 360

Query: 361 NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA 420
           NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA
Sbjct: 361 NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA 420

Query: 421 IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL 480
           IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL
Sbjct: 421 IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL 480

Query: 481 SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG 540
           SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG
Sbjct: 481 SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG 540

Query: 541 AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA 600
           AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA
Sbjct: 541 AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA 600

Query: 601 TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP 660
           TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP
Sbjct: 601 TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP 660

Query: 661 IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV 720
           IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV
Sbjct: 661 IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV 720

Query: 721 FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS 780
           FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS
Sbjct: 721 FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS 780

Query: 781 LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN 840
           LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN
Sbjct: 781 LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN 840

Query: 841 TTN 843
           TTN
Sbjct: 841 TTN 843

BLAST of MC01g1630 vs. NCBI nr
Match: XP_038883866.1 (subtilisin-like protease SBT2.2 [Benincasa hispida])

HSP 1 Score: 1588 bits (4112), Expect = 0.0
Identity = 792/844 (93.84%), Postives = 826/844 (97.87%), Query Frame = 0

Query: 1   MDIIHCARLLCVV-CFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIHCARLLCVV CFGMFLCASCLDEF DSTAVYIVTLKEP + THYYG+LR+NTTSFR
Sbjct: 1   MDIIHCARLLCVVVCFGMFLCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSFR 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
           +GTSGGLSIHKPRNISR HRRY SYIAR+HDSLL+KVLKGEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGTSGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGFID
Sbjct: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDLTDNPFP+P+HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NASQDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDT YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+N TNVS+DMYVGECQD+SNFD+D V+GNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNDTNVSEDMYVGECQDSSNFDQDLVQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTKKISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS++APQ+MYYSARGPDPEDSSLDDSDI+KPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSTAPQVMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSY+DYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYNDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSI+GADLNLPSVTIAKLNQSRVVQR+VTNIAGPEFYSVGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSITGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEFYSVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSP RF+IG+GEKQ LTIFFNATMNSSVASFGRIGLFGS GHI+NIPLSVI KISYN
Sbjct: 781 SLKVSPIRFTIGSGEKQELTIFFNATMNSSVASFGRIGLFGSAGHIINIPLSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 844

BLAST of MC01g1630 vs. NCBI nr
Match: XP_011658335.1 (subtilisin-like protease SBT2.3 [Cucumis sativus] >KGN49497.1 hypothetical protein Csa_004413 [Cucumis sativus])

HSP 1 Score: 1563 bits (4046), Expect = 0.0
Identity = 779/844 (92.30%), Postives = 819/844 (97.04%), Query Frame = 0

Query: 1   MDIIHCARLLCVV-CFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIHC RLLCVV CFG+F+CASCLDEF DSTAVYIVTLKEP + THYYG+LR+NTTSF 
Sbjct: 1   MDIIHCTRLLCVVVCFGVFVCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSF- 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
             TSGGLSIHK RNISR HRRY SYIAR+HDSLL+KVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  -STSGGLSIHKARNISRKHRRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR+EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGFID
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDLTDNPFPIP+HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLTDNPFPIPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNN+TIPGVGLAPGTYNDT YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNITIPGVGLAPGTYNDTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+N T+VS+DMYVGECQD+SNFD++ +EGNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGII+SSPEDSK+LLQYYNSSLEVDGLTKKISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIVSSPEDSKMLLQYYNSSLEVDGLTKKISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQK+PS SPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKYPSFSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSY DYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYSDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSI+GADLNLPSVTIAKLNQSRVVQR+VTNIAGPEFYSVGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSITGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEFYSVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSP RF+IG+GEKQ LTIFFN+TMNSSVASFGRIGLFGS GHI+NIPLSVI KISYN
Sbjct: 781 SLKVSPIRFTIGSGEKQELTIFFNSTMNSSVASFGRIGLFGSAGHIINIPLSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 842

BLAST of MC01g1630 vs. NCBI nr
Match: XP_022925849.1 (subtilisin-like protease SBT2.2 [Cucurbita moschata])

HSP 1 Score: 1560 bits (4038), Expect = 0.0
Identity = 778/844 (92.18%), Postives = 818/844 (96.92%), Query Frame = 0

Query: 1   MDIIHCARLLC-VVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIH ARLLC VVCFGMF+CASCLDEF DSTAVYIVTLKEP   THYYG+LR+NTTSFR
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPPTTHYYGQLRQNTTSFR 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
           +G SGGLSIHKPRNISR HRRY SYIAR+HDSLL+KVLKGEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR+EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGF+D
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFVD 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDL+D PFP+P HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN T  KL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKNKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+NGTNVS DMYVGECQD+SN+D+D ++GNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNYDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGNSIWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGE+FAMMSGTSMAAPHIAGLASL+KQK+ SLSPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGESFAMMSGTSMAAPHIAGLASLVKQKYSSLSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAAL+PGLIFDSSYDDYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALDPGLIFDSSYDDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSISGADLNLPSVTIAKLNQSRVVQR+VTNIAGPE Y+VGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSISGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEIYNVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSPTRF+I +G+KQ LTIF NATMNSSVASFGRIGLFGS GHI+NIP+SVI KISYN
Sbjct: 781 SLKVSPTRFTIDSGKKQELTIFLNATMNSSVASFGRIGLFGSAGHIINIPVSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 844

BLAST of MC01g1630 vs. NCBI nr
Match: XP_023543118.1 (subtilisin-like protease SBT2.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1559 bits (4037), Expect = 0.0
Identity = 776/844 (91.94%), Postives = 818/844 (96.92%), Query Frame = 0

Query: 1   MDIIHCARLLC-VVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIH ARLLC VVCFGMF+CASCLDEF DSTAVYIVTLKEP   THYYG+LR+NTTSFR
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPPTTHYYGQLRQNTTSFR 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
           +G SGGLSIHKPRNISR HRRY SYIAR+HDSLL+KVLKGEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR+EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGF+D
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFVD 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDL+D PFP+P HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN T YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+NGTNVS DMYVGECQD+SN+D+D ++GNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNYDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGNSIWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGE+FAMMSGTSMAAPHIAGLASL+KQK+ SLSPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGESFAMMSGTSMAAPHIAGLASLVKQKYSSLSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAY NPEQNQSPATPFDMGSGFVNATAAL+PGLIFDSSYDDYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYVNPEQNQSPATPFDMGSGFVNATAALDPGLIFDSSYDDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSISGADLNLPSVTIAKLNQSRVVQR+VTN+AGPE Y+VGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSISGADLNLPSVTIAKLNQSRVVQRTVTNLAGPEIYNVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSPT+F+I +G+KQ LTIF NATMNSSVASFGRIGLFGS GHI+NIP+SVI KISYN
Sbjct: 781 SLKVSPTQFTIDSGKKQELTIFLNATMNSSVASFGRIGLFGSAGHIINIPVSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 844

BLAST of MC01g1630 vs. ExPASy TrEMBL
Match: A0A6J1CXP3 (subtilisin-like protease SBT2.3 OS=Momordica charantia OX=3673 GN=LOC111015762 PE=3 SV=1)

HSP 1 Score: 1672 bits (4331), Expect = 0.0
Identity = 843/843 (100.00%), Postives = 843/843 (100.00%), Query Frame = 0

Query: 1   MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI 60
           MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI
Sbjct: 1   MDIIHCARLLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRI 60

Query: 61  GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT 120
           GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT
Sbjct: 61  GTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVT 120

Query: 121 QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT 180
           QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT
Sbjct: 121 QEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDT 180

Query: 181 GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN 240
           GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN
Sbjct: 181 GIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFN 240

Query: 241 ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG 300
           ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG
Sbjct: 241 ASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFG 300

Query: 301 GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG 360
           GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG
Sbjct: 301 GFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAG 360

Query: 361 NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA 420
           NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA
Sbjct: 361 NTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAA 420

Query: 421 IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL 480
           IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL
Sbjct: 421 IHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNL 480

Query: 481 SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG 540
           SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG
Sbjct: 481 SAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFG 540

Query: 541 AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA 600
           AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA
Sbjct: 541 AVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVA 600

Query: 601 TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP 660
           TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP
Sbjct: 601 TDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGP 660

Query: 661 IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV 720
           IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV
Sbjct: 661 IMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVV 720

Query: 721 FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS 780
           FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS
Sbjct: 721 FNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGIS 780

Query: 781 LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN 840
           LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN
Sbjct: 781 LKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYNN 840

Query: 841 TTN 843
           TTN
Sbjct: 841 TTN 843

BLAST of MC01g1630 vs. ExPASy TrEMBL
Match: A0A0A0KMB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G526310 PE=3 SV=1)

HSP 1 Score: 1563 bits (4046), Expect = 0.0
Identity = 779/844 (92.30%), Postives = 819/844 (97.04%), Query Frame = 0

Query: 1   MDIIHCARLLCVV-CFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIHC RLLCVV CFG+F+CASCLDEF DSTAVYIVTLKEP + THYYG+LR+NTTSF 
Sbjct: 1   MDIIHCTRLLCVVVCFGVFVCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSF- 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
             TSGGLSIHK RNISR HRRY SYIAR+HDSLL+KVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  -STSGGLSIHKARNISRKHRRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR+EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGFID
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDLTDNPFPIP+HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLTDNPFPIPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNN+TIPGVGLAPGTYNDT YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNITIPGVGLAPGTYNDTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+N T+VS+DMYVGECQD+SNFD++ +EGNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGII+SSPEDSK+LLQYYNSSLEVDGLTKKISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIVSSPEDSKMLLQYYNSSLEVDGLTKKISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQK+PS SPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKYPSFSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSY DYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYSDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSI+GADLNLPSVTIAKLNQSRVVQR+VTNIAGPEFYSVGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSITGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEFYSVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSP RF+IG+GEKQ LTIFFN+TMNSSVASFGRIGLFGS GHI+NIPLSVI KISYN
Sbjct: 781 SLKVSPIRFTIGSGEKQELTIFFNSTMNSSVASFGRIGLFGSAGHIINIPLSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 842

BLAST of MC01g1630 vs. ExPASy TrEMBL
Match: A0A6J1EDC1 (subtilisin-like protease SBT2.2 OS=Cucurbita moschata OX=3662 GN=LOC111433134 PE=3 SV=1)

HSP 1 Score: 1560 bits (4038), Expect = 0.0
Identity = 778/844 (92.18%), Postives = 818/844 (96.92%), Query Frame = 0

Query: 1   MDIIHCARLLC-VVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIH ARLLC VVCFGMF+CASCLDEF DSTAVYIVTLKEP   THYYG+LR+NTTSFR
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPPTTHYYGQLRQNTTSFR 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
           +G SGGLSIHKPRNISR HRRY SYIAR+HDSLL+KVLKGEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR+EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGF+D
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFVD 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDL+D PFP+P HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN T  KL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKNKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+NGTNVS DMYVGECQD+SN+D+D ++GNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNYDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGNSIWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGE+FAMMSGTSMAAPHIAGLASL+KQK+ SLSPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGESFAMMSGTSMAAPHIAGLASLVKQKYSSLSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAAL+PGLIFDSSYDDYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALDPGLIFDSSYDDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSISGADLNLPSVTIAKLNQSRVVQR+VTNIAGPE Y+VGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSISGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEIYNVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSPTRF+I +G+KQ LTIF NATMNSSVASFGRIGLFGS GHI+NIP+SVI KISYN
Sbjct: 781 SLKVSPTRFTIDSGKKQELTIFLNATMNSSVASFGRIGLFGSAGHIINIPVSVILKISYN 840

Query: 841 NTTN 843
           NTTN
Sbjct: 841 NTTN 844

BLAST of MC01g1630 vs. ExPASy TrEMBL
Match: A0A6J1IJ51 (subtilisin-like protease SBT2.3 OS=Cucurbita maxima OX=3661 GN=LOC111477908 PE=3 SV=1)

HSP 1 Score: 1553 bits (4021), Expect = 0.0
Identity = 774/844 (91.71%), Postives = 816/844 (96.68%), Query Frame = 0

Query: 1   MDIIHCARLLC-VVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDIIH ARLLC VVCFGMF+CASCLDEF DSTAVYIVTLKEP   THYYG+LR+NTTSFR
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPT-THYYGQLRQNTTSFR 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
           +G SGGLSIHKPRN+SR HRRY SYIAR+HDSLL+KVLKGEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNVSRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T++QANKLSKR EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIV+GF+D
Sbjct: 121 TEQQANKLSKRMEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVMGFVD 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDL+D PFP+P HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDII+LSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN T YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+NGTNVS DMYVGECQD+SNFD+D ++GNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNFDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGNSIWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGE+FAMMSGTSMAAPHIAGLASL+KQK+PS SPSAIASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGESFAMMSGTSMAAPHIAGLASLVKQKYPSFSPSAIASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAAL+PGLIFDSSYDDYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALDPGLIFDSSYDDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYTGQNC +YNSSISGADLNLPSVTIAKLNQSRVVQR+VTNIAGPE Y+VGWSAPYGI
Sbjct: 721 VFNYTGQNCGLYNSSISGADLNLPSVTIAKLNQSRVVQRTVTNIAGPEIYNVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSPTRF+I +G+KQ LTIF N TMNSSVASFGRIGLFGS GHI+NIP+SVI KISYN
Sbjct: 781 SLKVSPTRFTIDSGKKQELTIFLNVTMNSSVASFGRIGLFGSAGHIINIPVSVILKISYN 840

Query: 841 NTTN 843
           NT N
Sbjct: 841 NTAN 843

BLAST of MC01g1630 vs. ExPASy TrEMBL
Match: A0A1S3AZJ6 (subtilisin-like protease SBT2.3 OS=Cucumis melo OX=3656 GN=LOC103484283 PE=3 SV=1)

HSP 1 Score: 1553 bits (4020), Expect = 0.0
Identity = 777/844 (92.06%), Postives = 817/844 (96.80%), Query Frame = 0

Query: 1   MDIIHCARLLCVV-CFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFR 60
           MDII+CARLLCVV CFGMF+CASCLDEF DSTAVYIVTLKEP +ATHYYG+LR+NTTSF 
Sbjct: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSF- 60

Query: 61  IGTSGGLSIHKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLV 120
             TSGGLSIHK RN SR H+RY SYIAR+HDSLL+KVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  -STSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFID 180
           T+EQANKLSKR EVANVV+DFSVRTATTHTPQFLGLPQGAWSQDGG+ESAGAGIVIGFID
Sbjct: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180

Query: 181 TGIDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240
           TGIDP+HPSFADDLTDNPFP+P+HFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASGMAPRSHIAVYKALYKSF
Sbjct: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360
           GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDT YKL+A
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420

Query: 421 AIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKN 480
           AIHAL+N T+VS+DMYVGECQD+SNFD++ +EGNLLICSYSIRFVLGLSTVK+ALQTAKN
Sbjct: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKF 540
           LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSK+LLQYYNSSLEVDGLTKKISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540

Query: 541 GAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSV 600
           GAVA I GGLKANYS+SAPQIMYYSARGPDPEDSSLDDSDI+KPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 600

Query: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGG 660
           ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQK+PSLSPSA+ASALSTTASLYD+TGG
Sbjct: 601 ATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKYPSLSPSAVASALSTTASLYDKTGG 660

Query: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPV 720
           PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSY DYMSFLCGINGS+PV
Sbjct: 661 PIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYSDYMSFLCGINGSSPV 720

Query: 721 VFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGI 780
           VFNYT QNC +YNSSI+GADLNLPSVTIAKLNQSRVV+R+VTNIAGPEFYSVGWSAPYGI
Sbjct: 721 VFNYTAQNCGLYNSSITGADLNLPSVTIAKLNQSRVVRRTVTNIAGPEFYSVGWSAPYGI 780

Query: 781 SLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKISYN 840
           SLKVSP RF+I +GEKQ LTIFFNATMNSSVASFGRIGLFGS GHI+NIPLSVI KISYN
Sbjct: 781 SLKVSPVRFTISSGEKQELTIFFNATMNSSVASFGRIGLFGSAGHIINIPLSVILKISYN 840

Query: 841 NTTN 843
            TTN
Sbjct: 841 YTTN 842

BLAST of MC01g1630 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 581/844 (68.84%), Postives = 695/844 (82.35%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCLDEFDDS-----TAVYIVTLKEPSAATHYYGELRKNTTSFRIGTS 68
           +L + CFG+   +   D   DS     TAVYIVTL++ S + H + +  +     R  + 
Sbjct: 11  VLLLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQAS-SLHLFQQEAEEVKRVRDQSK 70

Query: 69  GGLSIH------KPRNISRTH--RRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGF 128
            G +        +PRNISR+   R   S IA+ HDSLLR  LKGEKY+KLYS+H+LINGF
Sbjct: 71  HGDTSKFTRPKLQPRNISRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGF 130

Query: 129 AVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVI 188
           AV V+ +QA  LS+RREVAN+VLDFSVRTATT+TPQF+GLP+GAW ++GGYE+AG GIVI
Sbjct: 131 AVFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVI 190

Query: 189 GFIDTGIDPTHPSF-ADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAI 248
           GFIDTGIDPTHPSF   D +   +PIP+HFSG+CEVTPDFPSGSCNRKLVGARHFA SAI
Sbjct: 191 GFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAI 250

Query: 249 TRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKA 308
           TRGIFN+S+DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG+APR+HI+VYKA
Sbjct: 251 TRGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKA 310

Query: 309 LYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIF 368
           LYKSFGGFAADVVAA+DQAAQDGVDI+SLSITPNRRPPG+ATFFNP+DMA+LSAVKAGIF
Sbjct: 311 LYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIF 370

Query: 369 VVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTM 428
           VVQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T     
Sbjct: 371 VVQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKK 430

Query: 429 YKLVAAIHALSNGTNVSD-DMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRA 488
           Y +++A+ AL N ++V D DMYVGECQD  +FD+D + GNLLICSYSIRFVLGLST+K+A
Sbjct: 431 YTMISALDALKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQA 490

Query: 489 LQTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLT 548
           L  AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG T
Sbjct: 491 LAVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTT 550

Query: 549 KKISKFGAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIW 608
           K+I +FGAVA I+GG  AN+S  AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGNSIW
Sbjct: 551 KEIVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIW 610

Query: 609 AAWSSVATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASL 668
            AWSS AT+S EF GE+FAMMSGTSMAAPH+AG+A+L+KQKF   SPSAIASALSTT+ L
Sbjct: 611 GAWSSAATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVL 670

Query: 669 YDRTGGPIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGI 728
           +D  G  IMAQRAYANP+Q  SPATPFDMG+GFVNATAAL+PGLIFD+S++DYMSFLCGI
Sbjct: 671 FDNKGEAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGI 730

Query: 729 NGSAPVVFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGW 788
           NGSAPVVFNYTG NC   N++ISG+DLNLPS+T++KLN +R VQR +TNIAG E Y+V  
Sbjct: 731 NGSAPVVFNYTGTNCLRNNATISGSDLNLPSITVSKLNNTRTVQRLMTNIAGNETYTVSL 790

Query: 789 SAPYGISLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVI 838
             P+ + + VSPT+FSI +GE ++L++   A  NSS++SFG I L G+ GHIV IP+SV 
Sbjct: 791 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 850

BLAST of MC01g1630 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 568/836 (67.94%), Postives = 685/836 (81.94%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCL---DEFDDSTAVYIVTLKEPSAATHYY--GELRKNTTSFRIGTS 68
           LL ++ F +FL  + L    + DD +AVYIVTLK+P    H +   EL+   + F     
Sbjct: 12  LLLMISF-VFLSNNTLGQQQDDDDDSAVYIVTLKQP-PIVHLFEEQELKHKKSKFTPKL- 71

Query: 69  GGLSIHKPRNISRTHRRYSSY--IARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVTQ 128
                 +PRN SR     S    + + HDS LRK LKGEKY+KLYSYH+LINGFA+ +  
Sbjct: 72  ------RPRNNSRKRHGKSKIPSVVQSHDSFLRKTLKGEKYIKLYSYHYLINGFALFINS 131

Query: 129 EQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDTG 188
           +QA KLS R+EVAN+VLD+SVRTATT+TPQF+GLPQGAW ++GG+E AG G++IGFIDTG
Sbjct: 132 QQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTG 191

Query: 189 IDPTHPSFADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 248
           IDP HPSF D+ +   +PIP HFSG+CEVTPDFPSGSCN+KL+GARHFA SA+TRGIFN+
Sbjct: 192 IDPNHPSFNDNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNS 251

Query: 249 SQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFGG 308
           S+DYASPFDGDGHGTHTAS+AAGNHG+PV+V+ H+FG ASG+APR+ I+VYKALYKSFGG
Sbjct: 252 SEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPRAFISVYKALYKSFGG 311

Query: 309 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGN 368
           FAADVVAA+DQAAQDGVDI+SLSITPNR+PPG+ATFFNPIDMALLSAVKAGIFVVQAAGN
Sbjct: 312 FAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALLSAVKAGIFVVQAAGN 371

Query: 369 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAAI 428
           TGPAPK+MSSFSPWIFTVGA+SHDR Y+NS++LGNNVTIPG+G A  T +  MYK+++A 
Sbjct: 372 TGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAF 431

Query: 429 HALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLS 488
           HAL+N T+V  DMYVGECQD  NFD+DRV G LLICSYS RFVLGLST+K+AL  AKNLS
Sbjct: 432 HALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIKQALDVAKNLS 491

Query: 489 AAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFGA 548
           A GVIFY+D +V+GF +NP PM MPGIII S EDSK LL+YYNSS++ D  TK+I  FGA
Sbjct: 492 ATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGA 551

Query: 549 VACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVAT 608
           VA I GGL AN+S  AP++MYYSARGPDPED+S +D+D++KPNLVAPGNSIW AWSS +T
Sbjct: 552 VAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSAST 611

Query: 609 DSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGPI 668
           DS EF GE FAMMSGTSMAAPH+AG+A+LIKQ +P  +PS I+SALSTTA L D  G PI
Sbjct: 612 DSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKGSPI 671

Query: 669 MAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVVF 728
           MAQR Y+NP+Q+   ATP DMGSGFVNATAAL+PGL+FD+S++DY+SFLCGINGS  VVF
Sbjct: 672 MAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSDTVVF 731

Query: 729 NYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWSAPYGISL 788
           NYTG  C   N+ +SG DLNLPS+T++ L+ ++  QRS+ NIAG E Y+VGWS PYG+S+
Sbjct: 732 NYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSM 791

Query: 789 KVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKIS 838
           KVSPT+FSI  GE QVL++    T NSS +SFGRIGLFG+ GHIVNIP++VI+KI+
Sbjct: 792 KVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKIA 838

BLAST of MC01g1630 vs. TAIR 10
Match: AT4G20430.2 (Subtilase family protein )

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 563/843 (66.79%), Postives = 675/843 (80.07%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCLDEFDDS-----TAVYIVTLKEPSAATHYYGELRKNTTSFRIGTS 68
           +L + CFG+   +   D   DS     TAVYIVTL++ S + H + +  +     R  + 
Sbjct: 11  VLLLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQAS-SLHLFQQEAEEVKRVRDQSK 70

Query: 69  GGLSIH------KPRNISRTH--RRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGF 128
            G +        +PRNISR+   R   S IA+ HDSLLR  LKGEKY+KLYS+H+LINGF
Sbjct: 71  HGDTSKFTRPKLQPRNISRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGF 130

Query: 129 AVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVI 188
           AV V+ +QA  LS+RREVAN+VLDFSVRTATT+TPQF+GLP+GAW ++GGYE+AG GIVI
Sbjct: 131 AVFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVI 190

Query: 189 GFIDTGIDPTHPSF-ADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAI 248
           GFIDTGIDPTHPSF   D +   +PIP+HFSG+CEVTPDFPSGSCNRKLVGARHFA SAI
Sbjct: 191 GFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAI 250

Query: 249 TRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKA 308
           TRGIFN+S+DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG+APR+HI+VYKA
Sbjct: 251 TRGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKA 310

Query: 309 LYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIF 368
           LYKSFGGFAADVVAA+DQAAQDGVDI+SLSITPNRRPPG+ATFFNP+DMA+LSAVKAGIF
Sbjct: 311 LYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIF 370

Query: 369 VVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTM 428
           VVQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T     
Sbjct: 371 VVQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKK 430

Query: 429 YKLVAAIHALSNGTNVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRAL 488
           Y +++A+ AL N ++V               D+D          YSIRFVLGLST+K+AL
Sbjct: 431 YTMISALDALKNKSSV--------------VDKD---------IYSIRFVLGLSTIKQAL 490

Query: 489 QTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTK 548
             AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG TK
Sbjct: 491 AVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTK 550

Query: 549 KISKFGAVACISGGLKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWA 608
           +I +FGAVA I+GG  AN+S  AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGNSIW 
Sbjct: 551 EIVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWG 610

Query: 609 AWSSVATDSIEFLGENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLY 668
           AWSS AT+S EF GE+FAMMSGTSMAAPH+AG+A+L+KQKF   SPSAIASALSTT+ L+
Sbjct: 611 AWSSAATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLF 670

Query: 669 DRTGGPIMAQRAYANPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGIN 728
           D  G  IMAQRAYANP+Q  SPATPFDMG+GFVNATAAL+PGLIFD+S++DYMSFLCGIN
Sbjct: 671 DNKGEAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGIN 730

Query: 729 GSAPVVFNYTGQNCRVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGPEFYSVGWS 788
           GSAPVVFNYTG NC   N++ISG+DLNLPS+T++KLN +R VQR +TNIAG E Y+V   
Sbjct: 731 GSAPVVFNYTGTNCLRNNATISGSDLNLPSITVSKLNNTRTVQRLMTNIAGNETYTVSLI 790

Query: 789 APYGISLKVSPTRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVIS 838
            P+ + + VSPT+FSI +GE ++L++   A  NSS++SFG I L G+ GHIV IP+SV  
Sbjct: 791 TPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVTV 829

BLAST of MC01g1630 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 1073.2 bits (2774), Expect = 1.2e-313
Identity = 546/832 (65.62%), Postives = 663/832 (79.69%), Query Frame = 0

Query: 9   LLCVVCFGMFLCASCLDEFDDSTAVYIVTLKEPSAATHYYGELRKNTTSFRIGTSGGLSI 68
           LLC+V   +F  A        S+AVYIVTLK+   + H+ G    ++      TS     
Sbjct: 12  LLCLVSSSVFCLAESDQNATVSSAVYIVTLKD-RPSVHFSGRESSDSKHSLTATSS---- 71

Query: 69  HKPRNISRTHRRYSSYIARIHDSLLRKVLKGEKYLKLYSYHFLINGFAVLVTQEQANKLS 128
                I RT  R S+ I R+HDSLLR VL+ E YLKLYSYH+LINGF+ ++T++QA++L+
Sbjct: 72  ----QIYRTLNR-SASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLA 131

Query: 129 KRREVANVVLDFSVRTATTHTPQFLGLPQGAWSQDGGYESAGAGIVIGFIDTGIDPTHPS 188
            R EV NVVLDF V  ATTHTPQFLGLP+GAW +DGG E AG G+VIGFIDTGIDPTHPS
Sbjct: 132 AREEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPS 191

Query: 189 FADDLTDNPFPIPSHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNASQDYASP 248
           F+D ++ + + +P HF+G+CEVT  FP GSCNRKL+GARHFA SA++RG+ N+SQD ASP
Sbjct: 192 FSDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASP 251

Query: 249 FDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVA 308
           FDG+GHGTHTAS+AAGNHGIPV+VAGH  GNASGMAPR+HIA+YKALYK FGGFAAD++A
Sbjct: 252 FDGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIA 311

Query: 309 AVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS 368
           A+DQAAQDGVDII+LSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS
Sbjct: 312 AIDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKS 371

Query: 369 MSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTMYKLVAAIHALSNGT 428
           MSSFSPWIFTVGA SHDR Y+NSI LGNNVTIPGVGLA GT    M+KLV A HAL NGT
Sbjct: 372 MSSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT--RIMHKLVLATHALRNGT 431

Query: 429 NVSDDMYVGECQDASNFDRDRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLSAAGVIFY 488
            V D +YVGECQD+S+FD+  V+G +L+CSY++RF+LG+ST+K+AL TAKNL+AAG++FY
Sbjct: 432 TVMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFY 491

Query: 489 MDSFVIGFRLNPIPMKMPGIIISSPEDSKILLQYYNSSLEVDGLTKKISKFGAVACISGG 548
           +D    GF++   PM +PGI+ISSP+DS+ LL+YYNSSL  +  + KI    +VA I GG
Sbjct: 492 IDPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGG 551

Query: 549 LKANYSTSAPQIMYYSARGPDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVATDSIEFLG 608
           ++  Y  +AP++MY+SARGPDPED S  D+DI+KPNLVAPGN+IW AWS +   + +F G
Sbjct: 552 MRPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQG 611

Query: 609 ENFAMMSGTSMAAPHIAGLASLIKQKFPSLSPSAIASALSTTASLYDRTGGPIMAQRAYA 668
           E FAM SGTSM+APH+ G+A+LIKQKFP  +P+AIASALSTTASL DR G  IMAQR   
Sbjct: 612 ERFAMESGTSMSAPHVTGIAALIKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVL 671

Query: 669 NPEQNQSPATPFDMGSGFVNATAALNPGLIFDSSYDDYMSFLCGINGSAPVVFNYTGQNC 728
           NP+ +QSPATPFDMGSGFVNATAAL+PGLIFD  Y++YM FLCGINGS+PVV NYTG++C
Sbjct: 672 NPDISQSPATPFDMGSGFVNATAALDPGLIFDIGYNEYMKFLCGINGSSPVVLNYTGESC 731

Query: 729 RVYNSSISGADLNLPSVTIAKLNQSRVVQRSVTNIAGP---EFYSVGWSAPYGISLKVSP 788
             YNSS++ +DLNLPSVTIAKL  +R V R VTNIA     E Y VGW AP  +S+KVSP
Sbjct: 732 SSYNSSLAASDLNLPSVTIAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSP 791

Query: 789 TRFSIGNGEKQVLTIFFNATMNSSVASFGRIGLFGSEGHIVNIPLSVISKIS 838
            +F+IGNG+ +VL++ F A  N S+ASFGRIGLFG  GH+VNIP++VI KI+
Sbjct: 792 AKFTIGNGQTRVLSLVFRAMKNVSMASFGRIGLFGDRGHVVNIPVAVIYKIA 831

BLAST of MC01g1630 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 650.2 bits (1676), Expect = 2.3e-186
Identity = 369/805 (45.84%), Postives = 494/805 (61.37%), Query Frame = 0

Query: 33  VYIVTLKEPSAATHYYGELRKNTTSFRIGTSGGLSIHKPRNISRTHRRYSSYIARIHDSL 92
           VYIVT++     ++  GE     T+         S  K    S     Y+ ++ R HD +
Sbjct: 21  VYIVTMEGDPIISYKGGENGFEATAVE-------SDEKIDTSSELVTVYARHLERKHDMI 80

Query: 93  LRKVLKGEKYLKLYSYHFLINGFAVLVTQEQANKLSKRREVANVVLDFSVRTATTHTPQF 152
           L  + +   Y KLYSY  LINGFA  V+ EQA  L +   V +V  D+ VR  TTHTP+F
Sbjct: 81  LGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEF 140

Query: 153 LGLPQGAWSQDGGYESAGAGIVIGFIDTGIDPTHPSFADDLTDNPFPIPSHFSGICEVTP 212
           LGLP   W   GG++ AG  IVIGF+D+GI P HPSFA        P+P H+ G CE  P
Sbjct: 141 LGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLP-HYKGKCEEDP 200

Query: 213 DFPSGSCNRKLVGARHFAASAITRGIFNASQDYASPFDGDGHGTHTASIAAGNHGIPVLV 272
                 CNRK+VGA+HFA +A   G FN   DYASP DGDGHG+HTA+IAAGN+GIP+ +
Sbjct: 201 HTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRM 260

Query: 273 AGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVAAVDQAAQDGVDIISLSITPNRRPP 332
            G+ FG ASGMAPR+ IAVYKALY+ FGGF ADVVAA+DQA  DGVDI+SLS+ PN  P 
Sbjct: 261 HGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPT 320

Query: 333 GI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANS 392
               TF NP D  LL AVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N 
Sbjct: 321 TTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNH 380

Query: 393 ISLGNNVTIPGVGLAPGTYNDTMYKLVAAIHALSNGT----NVSDDMYVGECQDASNFDR 452
           ++LGN   + G+GL+P T    +Y LV+A   L + +    N SD      CQ    F++
Sbjct: 381 LTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSD------CQRPEVFNK 440

Query: 453 DRVEGNLLICSYSIRFVLGLSTVKRALQTAKNLSAAGVIFYMDSFVIGFRLNPIPMKMPG 512
             VEGN+L+C YS  FV+G +++K+ + TAK+L AAG +  +++   G + +P+P  +PG
Sbjct: 441 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 500

Query: 513 IIISSPEDSKILLQYYNSSLEVDGLTKKISKFGAVACISGGLKANYSTSAPQIMYYSARG 572
           I+I+    S  L+ YYN+S   D  T ++  F A   I  GL      SAPQ+  +SARG
Sbjct: 501 ILITDVSKSMDLIDYYNASTSRD-WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARG 560

Query: 573 PDPEDSSLDDSDIVKPNLVAPGNSIWAAWSSVATDSIEFLGENFAMMSGTSMAAPHIAGL 632
           P+ +D S  D+D++KP+++APG  IWAAW    TD   ++GE FA++SGTSMAAPHIAG+
Sbjct: 561 PNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGI 620

Query: 633 ASLIKQKFPSLSPSAIASALSTTASLYDRTGGPIMAQRAYANPEQNQSPATPFDMGSGFV 692
           A+L+KQK P  SP+AI SAL TT+++ DR G  + AQ+           ATPFD GSG V
Sbjct: 621 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHV 680

Query: 693 NATAALNPGLIFDSSYDDYMSFLCGING-SAPVVFNYTGQNCRVYNSSISGADLNLPSVT 752
           N +AAL+PGLIFD+ Y+DY+ FLC   G SA  + NYT   C  Y+     ++ N PS+ 
Sbjct: 681 NPSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACN-YDMK-HPSNFNAPSIA 740

Query: 753 IAKLNQSRVVQRSVTNIAG-PEFYSVGWSAPYGISLKVSPTRFSIGNGEKQVLTIFFNAT 812
           ++ L  ++ V R VTN+A   E Y++       I+++V+P   ++  G  +  ++     
Sbjct: 741 VSHLVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVR 800

Query: 813 MNSSVASFGRIGLFGSEGHIVNIPL 831
             S V SFG + L GS GH V IP+
Sbjct: 801 SVSGVYSFGEVKLKGSRGHKVRIPV 808