MC01g1513 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1513 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 4.1e-110
Identity = 191/354 (53.95%), Postives = 264/354 (74.58%), Query Frame = 0

Query: 3   LQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADS--SAAQIPVIDMHKLLFSD 62
           ++LG+ L +P V+ELAK  L+ +P RY    + P      S      +PVID+  LL  +
Sbjct: 7   IKLGNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPE 66

Query: 63  HSEDS-ELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGD 122
                 ELDKLH ACK+WGFFQL+NHGV   L+++++S I+  F+LPM EK K  Q+ GD
Sbjct: 67  PVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGD 126

Query: 123 FEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAM 182
           FEGFGQ ++ SE+Q+L+W ++F +++LP +LRKPHLFP LPLPFR+ L++Y S+MK L+ 
Sbjct: 127 FEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLST 186

Query: 183 KLLDSMAKALKM-EGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQ 242
            + + + K+L++ E   M +LFE+G+Q +RMNYYPPCP+PELV+GL +HSD SG+TILLQ
Sbjct: 187 VVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQ 246

Query: 243 LNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVA 302
           LNE+EGLQIR + RWI +KPLP+AF+VNVGD++EI+TNGIY S+EHRA+VNS KERLS+A
Sbjct: 247 LNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIA 306

Query: 303 MFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
            F++ +L+ E+GP  SLVT E P LFKR R  D  +   S++L+GKS+LD MR+
Sbjct: 307 TFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of MC01g1513 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.4e-110
Identity = 192/350 (54.86%), Postives = 263/350 (75.14%), Query Frame = 0

Query: 8   SLPVPCVRELAK-GPLSTVPLRYVRLDQDPRFEFAD--SSAAQIPVIDMHKLLFSDHSED 67
           S+ VP V+E+ K   ++TVP RYVR DQD + E  D      +IP+IDM K L S  + D
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQD-KTEVDDDFDVKIEIPIIDM-KRLCSSTTMD 70

Query: 68  SELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFG 127
           SE++KL  ACK+WGFFQL+NHG+  + ++ ++S IQ+ F+LPMEEK+K WQ+  + EGFG
Sbjct: 71  SEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFG 130

Query: 128 QNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDS 187
           Q FVVSE+QKL+W DLF     P  LRKPHLFP LPLPFRD L+ Y+SE++ +A  L+  
Sbjct: 131 QAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAK 190

Query: 188 MAKALKMEGCEMRELFE--EGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEM 247
           MA+AL+++  E+ +LF+  + +Q +RMNYYPPCPQP+ VIGL  HSD+ G+T+L+Q+N++
Sbjct: 191 MARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDV 250

Query: 248 EGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYN 307
           EGLQI+ DG+W+PVKPLPNAF+VN+GDV+EI+TNG Y SIEHR +VNS KERLS+A F+N
Sbjct: 251 EGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHN 310

Query: 308 PRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
             +  E+GPA SLV  ++   FKR+ + +Y  G FS+ L+GK+YLD +RI
Sbjct: 311 VGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MC01g1513 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.0e-108
Identity = 192/358 (53.63%), Postives = 263/358 (73.46%), Query Frame = 0

Query: 3   LQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADS---SAAQIPVIDMHKLLFS 62
           ++LG+ + +P V+ELAK  L+ +P RY+   ++ +     S       +PVID+  L+ S
Sbjct: 7   MKLGNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISS 66

Query: 63  DH-SEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAG 122
           +  +E  ELD+LH ACK+WGFFQ++NHGV  +LV++++S IQ  F+L M EK K  QK G
Sbjct: 67  EPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDG 126

Query: 123 DFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLA 182
           D EGFGQ FV SE+Q L+W D+F I+TLP +LRKPHLF  LPLP R+ +++Y+SEMK L+
Sbjct: 127 DVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLS 186

Query: 183 MKLLDSMAKALKMEGCEMREL---FEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITI 242
           M L + M KAL+++  E++E+   F++  Q +RMNYYPPCPQPEL IGL  HSD  G+TI
Sbjct: 187 MVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTI 246

Query: 243 LLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERL 302
           LLQLNE+EGLQI+N+GRWI VKPLPNAFVVNVGDV+EI+TNG+Y S++HRA+VNS KERL
Sbjct: 247 LLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERL 306

Query: 303 SVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVM-DYFRGFFSKELNGKSYLDVMRI 353
           S+A F++P L+ E+GP  SL+T   P LF+      +    F S++L+GKS+LD MR+
Sbjct: 307 SIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of MC01g1513 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.2e-106
Identity = 189/358 (52.79%), Postives = 257/358 (71.79%), Query Frame = 0

Query: 3   LQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQD---PRFEFADSSAAQIPVIDMHKLLFS 62
           ++LG+ + +P V+ELAK  L+ +P RYV  +++   P      +    IPVID+  LL  
Sbjct: 7   MKLGNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSP 66

Query: 63  DHSEDS-ELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAG 122
           +      ELD+LH ACK+WGFFQ++NHGV  +LV+S++S IQ  F+L M+EK K  Q+ G
Sbjct: 67  EPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDG 126

Query: 123 DFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLA 182
           D EGFGQ F+ SE+Q L+W D+F + TLP +LRKPHLF  LP+P R+ +++Y+SEMK L+
Sbjct: 127 DVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMKKLS 186

Query: 183 MKLLDSMAKALKMEGCE---MRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITI 242
           M L + M KAL+++  E   M E+F +G Q +RMNYYPPCPQP L IGL +HSD  G+TI
Sbjct: 187 MVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTI 246

Query: 243 LLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERL 302
           LLQ+NE+EGLQI+ +G WI VKPLPNAFVVNVGD++EI+TNGIYHS++HRA+VNS  ERL
Sbjct: 247 LLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERL 306

Query: 303 SVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVM-DYFRGFFSKELNGKSYLDVMRI 353
           S+A F++P L+  +GP  SL+T E P LFK      D      +++L+GKS+LD MRI
Sbjct: 307 SIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMRI 364

BLAST of MC01g1513 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.2e-74
Identity = 148/351 (42.17%), Postives = 216/351 (61.54%), Query Frame = 0

Query: 5   LGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSDHSED 64
           +G SLPV  V+ LA   L  +P RYVR + +        ++ +IPVID+ +LL   ++ D
Sbjct: 8   VGGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACD 67

Query: 65  SELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFG 124
            EL K H AC DWGFFQLINHGV + ++E M+   ++ F LP +EK    Q     EG+G
Sbjct: 68  -ELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYG 127

Query: 125 QNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDS 184
           Q FV SEEQKL+W D+  +IT P   R    +P  P  FR+ ++ Y+ E++ +AM L   
Sbjct: 128 QAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCLTGM 187

Query: 185 MAKALKMEGCEMRELFEEGMQGI---RMNYYPPCPQPELVIGLNNHSDASGITILLQLNE 244
           MAK L +E     E+  + ++ +        P        +GL+ HSDA+G+T+L+Q+NE
Sbjct: 188 MAKNLGLES----EILTKPLRTVFNREDELLPSMSSCGEGLGLSPHSDATGLTLLIQVNE 247

Query: 245 MEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFY 304
           + GL I+ D +W+P+KP+  AFVVN+GDV+EI++NGIY SIEHRA++N+ KERLS+A F+
Sbjct: 248 VNGLHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSIAAFH 307

Query: 305 NPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
           +P    ++GP P LV  E    +K I   DY     + +L+GKS LD M++
Sbjct: 308 DPEYGTKIGPLPDLV-KENGVKYKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

BLAST of MC01g1513 vs. NCBI nr
Match: XP_022142068.1 (protein SRG1-like [Momordica charantia])

HSP 1 Score: 718 bits (1853), Expect = 3.83e-261
Identity = 354/356 (99.44%), Postives = 354/356 (99.44%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           MELQLGDSL VPCVRELAKGPLSTVPLRYVRLDQDP FEFADSSAAQIPVIDMHKLLFSD
Sbjct: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF
Sbjct: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK
Sbjct: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN
Sbjct: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF
Sbjct: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK 356
           YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK
Sbjct: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK 356

BLAST of MC01g1513 vs. NCBI nr
Match: KAG6581394.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 588 bits (1515), Expect = 1.37e-209
Identity = 286/353 (81.02%), Postives = 311/353 (88.10%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP FEF D+S  Q+PVIDMHKLL S+
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTST-QVPVIDMHKLLSSN 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED ELDK HHACKDWGFFQLINHGV+D LVESM+SGIQ LF+LPMEEK KLWQK GD 
Sbjct: 66  NCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQKPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLFPNLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVMRI+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQ 357

BLAST of MC01g1513 vs. NCBI nr
Match: XP_023544158.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584 bits (1505), Expect = 4.56e-208
Identity = 284/353 (80.45%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP F F D+SA Q+PVID+HKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFVFTDTSA-QVPVIDLHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED ELDK HHACKDWGFFQLINHGV+D LVESM+SGI+ LF+LPMEEK KLWQK GD 
Sbjct: 66  NCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIEALFNLPMEEKSKLWQKPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLFPNLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVMRI+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQ 357

BLAST of MC01g1513 vs. NCBI nr
Match: XP_022978724.1 (protein SRG1-like [Cucurbita maxima])

HSP 1 Score: 584 bits (1505), Expect = 4.56e-208
Identity = 285/353 (80.74%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP FEF D+SA Q+PVIDMHKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSA-QVPVIDMHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED ELDK HHACKDWGFFQLINHGV+D LVESM+SGIQ LF+LPMEEK KLWQK GD 
Sbjct: 66  NCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQKPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLF NLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFLNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVM+I+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMKIQ 357

BLAST of MC01g1513 vs. NCBI nr
Match: XP_022925780.1 (protein SRG1-like [Cucurbita moschata])

HSP 1 Score: 583 bits (1504), Expect = 6.47e-208
Identity = 284/353 (80.45%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP FEF D+S  Q+PVIDMHKLL S+
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTST-QVPVIDMHKLLSSN 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED EL K HHACKDWGFFQLINHGV+D LVESM+SGIQ LF+LPMEEK KLWQ+ GD 
Sbjct: 66  NCEDPELAKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQRPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLFPNLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVMRI+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQ 357

BLAST of MC01g1513 vs. ExPASy TrEMBL
Match: A0A6J1CMB4 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012287 PE=3 SV=1)

HSP 1 Score: 718 bits (1853), Expect = 1.85e-261
Identity = 354/356 (99.44%), Postives = 354/356 (99.44%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           MELQLGDSL VPCVRELAKGPLSTVPLRYVRLDQDP FEFADSSAAQIPVIDMHKLLFSD
Sbjct: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF
Sbjct: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK
Sbjct: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN
Sbjct: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF
Sbjct: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK 356
           YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK
Sbjct: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIEDTK 356

BLAST of MC01g1513 vs. ExPASy TrEMBL
Match: A0A6J1IUU4 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478595 PE=3 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 2.21e-208
Identity = 285/353 (80.74%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP FEF D+SA Q+PVIDMHKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTSA-QVPVIDMHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED ELDK HHACKDWGFFQLINHGV+D LVESM+SGIQ LF+LPMEEK KLWQK GD 
Sbjct: 66  NCEDPELDKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQKPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLF NLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFLNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVM+I+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMKIQ 357

BLAST of MC01g1513 vs. ExPASy TrEMBL
Match: A0A6J1EJ61 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433085 PE=3 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 3.13e-208
Identity = 284/353 (80.45%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSLPVPCV+EL K   STVP RY+R DQDP FEF D+S  Q+PVIDMHKLL S+
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTST-QVPVIDMHKLLSSN 65

Query: 61  HSEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDF 120
           + ED EL K HHACKDWGFFQLINHGV+D LVESM+SGIQ LF+LPMEEK KLWQ+ GD 
Sbjct: 66  NCEDPELAKFHHACKDWGFFQLINHGVSDVLVESMKSGIQALFNLPMEEKSKLWQRPGDV 125

Query: 121 EGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMK 180
           EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLFPNLPLPFRDDLD YA EMK LAMK
Sbjct: 126 EGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPHLFPNLPLPFRDDLDAYALEMKNLAMK 185

Query: 181 LLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLN 240
           LL+ MAKALKME CEMRE+FEEGM   RMNYYPPCPQPELVIGLNNHSDAS ITILLQ+N
Sbjct: 186 LLELMAKALKMEICEMREVFEEGMMSTRMNYYPPCPQPELVIGLNNHSDASAITILLQVN 245

Query: 241 EMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMF 300
           EMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+TNG Y SIEHRA VNS KERLSVAMF
Sbjct: 246 EMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEIITNGTYRSIEHRATVNSAKERLSVAMF 305

Query: 301 YNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 353
           Y+PRLDGE+GPAPSLVT ERP LFK I V D+ +GFF++ELNGKSYLDVMRI+
Sbjct: 306 YSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLKGFFTRELNGKSYLDVMRIQ 357

BLAST of MC01g1513 vs. ExPASy TrEMBL
Match: A0A5A7UBP9 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008610 PE=3 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 1.13e-204
Identity = 279/353 (79.04%), Postives = 313/353 (88.67%), Query Frame = 0

Query: 2   ELQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSDH 61
           +LQL DSLPVPCV+ELAK  LSTVPLRYVR DQDP FEF D+SA ++PVIDMHKLLFS++
Sbjct: 49  KLQLRDSLPVPCVQELAKSSLSTVPLRYVRPDQDPPFEFTDASA-EVPVIDMHKLLFSNN 108

Query: 62  SEDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFE 121
            +DSELDKLHHACKDWGFFQ+INHGV+D LVE+++SGIQ LF+LPM EKRKLWQ+ GD E
Sbjct: 109 FDDSELDKLHHACKDWGFFQVINHGVSDVLVENVKSGIQALFNLPMVEKRKLWQRPGDVE 168

Query: 122 GFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKL 181
           GFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHLFPNLPLPFRDDLD YA E+K L MKL
Sbjct: 169 GFGQSFVVSEEQKLNWGDLFGMFILPTYLRKPHLFPNLPLPFRDDLDAYALEIKNLGMKL 228

Query: 182 LDSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNE 241
            D MAKAL+M+  EMREL+EEGM   RMNYYPPCPQPELV+GLNNHSDAS ITILLQ+NE
Sbjct: 229 FDLMAKALEMDSSEMRELYEEGMLSTRMNYYPPCPQPELVMGLNNHSDASAITILLQVNE 288

Query: 242 MEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFY 301
           MEGLQIR DGRW PVKPLPNAFVVN+GDV+EIVTNGIY SIEHRA VNS KERLSVAMF+
Sbjct: 289 MEGLQIRKDGRWTPVKPLPNAFVVNIGDVLEIVTNGIYRSIEHRATVNSTKERLSVAMFF 348

Query: 302 NPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIED 354
           +PRLDGE+GPAPSLVT+ER  LFKRI V D+   FF +ELNG+SYLDVMRI++
Sbjct: 349 SPRLDGEIGPAPSLVTSERSALFKRIGVADFLNEFFKRELNGRSYLDVMRIQE 400

BLAST of MC01g1513 vs. ExPASy TrEMBL
Match: A0A6J1EL63 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433603 PE=3 SV=1)

HSP 1 Score: 566 bits (1459), Expect = 2.68e-201
Identity = 273/352 (77.56%), Postives = 311/352 (88.35%), Query Frame = 0

Query: 3   LQLGDSLPVPCVRELAKGPLSTVPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSDHS 62
           L+LGDSL VP V+ELAK   STVPLRYVR +QDP F+F D+S  QIPVID+HKLLFS++S
Sbjct: 8   LRLGDSLLVPSVQELAKSSPSTVPLRYVRPNQDPPFQFIDTSP-QIPVIDLHKLLFSNNS 67

Query: 63  EDSELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEG 122
           +D EL KLH ACKDWGFFQLINHGV+DALVE+M+SG++ LF+LP+EEKRKLWQK GDFEG
Sbjct: 68  QDCELHKLHLACKDWGFFQLINHGVSDALVENMKSGVEALFNLPLEEKRKLWQKPGDFEG 127

Query: 123 FGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLL 182
           FGQ+FV SEEQKLNWGDLF +  LP YLRKPHLFPNLPLPFRDDLD YA EM++LAMKLL
Sbjct: 128 FGQSFVFSEEQKLNWGDLFAVFILPAYLRKPHLFPNLPLPFRDDLDAYALEMEILAMKLL 187

Query: 183 DSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEM 242
           D MAK LKMEGCEMREL   G+   RMNYYPPCPQPELVIGLNNHSDASG+TILLQ+NEM
Sbjct: 188 DLMAKTLKMEGCEMRELLGGGIVSTRMNYYPPCPQPELVIGLNNHSDASGLTILLQVNEM 247

Query: 243 EGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYN 302
           EGLQIR DGRWIPV+PLPNAFVVN+GD++EIVTNGIY SIEHR  VNS KERLS+A+FY+
Sbjct: 248 EGLQIRKDGRWIPVQPLPNAFVVNIGDILEIVTNGIYRSIEHRVTVNSAKERLSLAIFYS 307

Query: 303 PRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIED 354
           PRLDGE+GPAPSLV+++ P LFKRI V D+ +GFFSKELNGKSYLD MRI++
Sbjct: 308 PRLDGEIGPAPSLVSSDSPALFKRIGVTDFLKGFFSKELNGKSYLDCMRIQN 358

BLAST of MC01g1513 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 412.1 bits (1058), Expect = 4.4e-115
Identity = 204/348 (58.62%), Postives = 257/348 (73.85%), Query Frame = 0

Query: 8   SLPVPCVRELAKGPLST--VPLRYVRLDQDPRFEFADSSAAQIPVIDMHKLLFSDHSEDS 67
           S+ VP V+E+ K  + T  +P RYVR DQ+      DS   QIP+IDM  LL S  S DS
Sbjct: 10  SVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDM-SLLSSSTSMDS 69

Query: 68  ELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQ 127
           E+DKL  ACK+WGFFQL+NHG+    ++  +S IQ+ F+LPMEEK+KLWQ+ GD EGFGQ
Sbjct: 70  EIDKLDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQ 129

Query: 128 NFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDSM 187
            FV SEEQKL+W D+F +   P  LRKPHLFP LPLPFRD LDTY++E+K +A  L   +
Sbjct: 130 AFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKL 189

Query: 188 AKALKMEGCEMRELFEEGM-QGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEMEG 247
           A ALK++  EM +LF++ + Q IRMNYYPPCP+P+  IGL  HSDA+G+TILLQ+NE+EG
Sbjct: 190 ASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEG 249

Query: 248 LQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYNPR 307
           LQI+ DG+W+ VKPLPNA VVNVGD++EI+TNG Y SIEHR +VNS KERLSVA F+N  
Sbjct: 250 LQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTG 309

Query: 308 LDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
              E+GP  SLV   +  LFK +   +YF G FS+EL+GK+YLDVMRI
Sbjct: 310 FGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of MC01g1513 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 198/348 (56.90%), Postives = 256/348 (73.56%), Query Frame = 0

Query: 8   SLPVPCVRELAKGP-LSTVPLRYVRLDQDPRFEFADSSAA-QIPVIDMHKLLFSDHSEDS 67
           S+ VP V+E+ K   ++TVP RYVR DQD      DS    QIP+IDM  LL S  S DS
Sbjct: 10  SIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDM-SLLCSSTSMDS 69

Query: 68  ELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQ 127
           E+DKL  ACK+WGFFQL+NHG+  + +  ++S +Q+ F+LPMEEK+ LWQ+  + EGFGQ
Sbjct: 70  EIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQ 129

Query: 128 NFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDSM 187
            FVVSEEQKL+W D+F +   P  LRKPHLFP LPLPFRD LD Y++E+K +A  LL  +
Sbjct: 130 VFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKI 189

Query: 188 AKALKMEGCEMRELFEEGM-QGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEMEG 247
           A ALK++  EM +LF++ + Q IR+NYYP CP+P+ VIGL  HSD++G+TILLQ NE+EG
Sbjct: 190 AVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVEG 249

Query: 248 LQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYNPR 307
           LQI+ + +W+ VKPLPNA VVNVGD++EI+TNG Y SIEHR +VNS KERLSVA F+N  
Sbjct: 250 LQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIG 309

Query: 308 LDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
           L  E+GP  SLV   +   FK +   +YF G FS+EL+GK+YLDVMR+
Sbjct: 310 LGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of MC01g1513 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-111
Identity = 192/350 (54.86%), Postives = 263/350 (75.14%), Query Frame = 0

Query: 8   SLPVPCVRELAK-GPLSTVPLRYVRLDQDPRFEFAD--SSAAQIPVIDMHKLLFSDHSED 67
           S+ VP V+E+ K   ++TVP RYVR DQD + E  D      +IP+IDM K L S  + D
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQD-KTEVDDDFDVKIEIPIIDM-KRLCSSTTMD 70

Query: 68  SELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFG 127
           SE++KL  ACK+WGFFQL+NHG+  + ++ ++S IQ+ F+LPMEEK+K WQ+  + EGFG
Sbjct: 71  SEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFG 130

Query: 128 QNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDS 187
           Q FVVSE+QKL+W DLF     P  LRKPHLFP LPLPFRD L+ Y+SE++ +A  L+  
Sbjct: 131 QAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAK 190

Query: 188 MAKALKMEGCEMRELFE--EGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEM 247
           MA+AL+++  E+ +LF+  + +Q +RMNYYPPCPQP+ VIGL  HSD+ G+T+L+Q+N++
Sbjct: 191 MARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDV 250

Query: 248 EGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYN 307
           EGLQI+ DG+W+PVKPLPNAF+VN+GDV+EI+TNG Y SIEHR +VNS KERLS+A F+N
Sbjct: 251 EGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHN 310

Query: 308 PRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
             +  E+GPA SLV  ++   FKR+ + +Y  G FS+ L+GK+YLD +RI
Sbjct: 311 VGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MC01g1513 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 398.3 bits (1022), Expect = 6.6e-111
Identity = 197/350 (56.29%), Postives = 258/350 (73.71%), Query Frame = 0

Query: 8   SLPVPCVRELAKGP-LSTVPLRYVRLDQDPRFEFADSSA--AQIPVIDMHKLLFSDHSED 67
           S+ VP V+E+ K   ++TVP RYVR DQD        S   ++IP+IDM++L  S  + D
Sbjct: 11  SILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLC-SSTAVD 70

Query: 68  SELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFG 127
           SE++KL  ACK++GFFQL+NHG+  + ++ ++S IQ+ F+LPMEEK+KLWQ     EGFG
Sbjct: 71  SEVEKLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFG 130

Query: 128 QNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDS 187
           Q FVVSE+QKL+W DLF +I  P  LRK HLFP LPLPFRD LD Y++ +K +A  LL  
Sbjct: 131 QAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLAK 190

Query: 188 MAKALKMEGCEMRELF-EEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEME 247
           MAKAL+++  E+ E+F ++ MQ +RMNYYPPCPQP LV GL  HSDA G+TILLQ+NE++
Sbjct: 191 MAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEVD 250

Query: 248 GLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYNP 307
           GLQI+ +G+W  VKPL NAF+VNVGDV+EI+TNG Y SIEHRA+VN  KERLS+A F+N 
Sbjct: 251 GLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHNT 310

Query: 308 RLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRIE 354
            +D E+GPA SLV  +    F+ ++  DY  G FS+EL GK+YLD MRIE
Sbjct: 311 GMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRIE 359

BLAST of MC01g1513 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 3.6e-109
Identity = 191/347 (55.04%), Postives = 261/347 (75.22%), Query Frame = 0

Query: 8   SLPVPCVRELAKGP-LSTVPLRYVRLDQDPRFEFADSS-AAQIPVIDMHKLLFSDHSEDS 67
           SL VP V E+ K    +T+P RYVR+DQ+      DSS +++IPVIDM +L  S  + DS
Sbjct: 11  SLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLC-SVSAMDS 70

Query: 68  ELDKLHHACKDWGFFQLINHGVTDALVESMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQ 127
           EL KL  AC+DWGFFQL+NHG+  + +E + + +QE F+LPM+EK+KLWQ++G+FEGFGQ
Sbjct: 71  ELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQ 130

Query: 128 NFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNLPLPFRDDLDTYASEMKVLAMKLLDSM 187
             +VSE QKL+WGD+F + T P   RK HLF  LP PFR+ L+TY+SE+K +A  L   M
Sbjct: 131 VNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKM 190

Query: 188 AKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPELVIGLNNHSDASGITILLQLNEMEGL 247
           A  L+++  EM +LF++  Q I++NYYPPCPQP+ V+GL  HSDA+G+TILLQ+N++EGL
Sbjct: 191 ASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGL 250

Query: 248 QIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNGIYHSIEHRAIVNSVKERLSVAMFYNPRL 307
           QI+ DG+W+ VKPL +A VVNVG+++EI+TNG Y SIEHRA+VNS KERLSVAMF++P  
Sbjct: 251 QIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGK 310

Query: 308 DGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSKELNGKSYLDVMRI 353
           +  + PA SLV  ++  LFK +   +YF  FF+++LNGKS+LD+MRI
Sbjct: 311 ETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356