MC01g1257 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1257 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 8.8e-53
Identity = 104/207 (50.24%), Postives = 142/207 (68.60%), Query Frame = 0

Query: 21  EEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSN 80
           + + +EEVW KHYSS H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS D + +KY  
Sbjct: 12  DSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKK 71

Query: 81  AIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLN 140
           A  N++ L+  G L+ +G+DA  +  H  LR +RFDR+++NFPH GF  RE     I+ +
Sbjct: 72  ARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKH 131

Query: 141 KELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYP 200
           +ELV GF  NG + L + +GE+HV+HK   P+ +W L E A +  L L + V F + +YP
Sbjct: 132 RELVFGFF-NGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYP 191

Query: 201 GYQNKRADGGQSDEPFRLGDCSTYKFK 228
           GY+NKR DG + D+PF LG+CST+KF+
Sbjct: 192 GYENKRGDGRRCDQPFLLGECSTFKFR 217

BLAST of MC01g1257 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.0e-49
Identity = 106/206 (51.46%), Postives = 135/206 (65.53%), Query Frame = 0

Query: 24  EEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIR 83
           E  E W +HYSS H+ILLVGEG+FSFSLCLA+ FGSA NI ATSLDS+D+L  KY +A+ 
Sbjct: 2   EISEKWIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVD 61

Query: 84  NVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVG--FLFREDSFCQIQLNK 143
           N+  L+  GC + + +D   MS    L  QR+DRIV+NFPH G  F  RE S   I+ +K
Sbjct: 62  NINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHK 121

Query: 144 ELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPG 203
           ELV GFL N K +L++ DGEIH+THK   P+  W + +     GLKL +   F    YPG
Sbjct: 122 ELVRGFLENAKEMLEE-DGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPG 181

Query: 204 YQNKRADGG-QSDEPFRLGDCSTYKF 227
           Y  KR  GG +SD+ F +G+CSTY F
Sbjct: 182 YITKRGSGGRRSDDYFPVGECSTYMF 206

BLAST of MC01g1257 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 6.0e-17
Identity = 66/200 (33.00%), Postives = 94/200 (47.00%), Query Frame = 0

Query: 38  RILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFY 97
           R+LLVGEG+FSF+  L +    + ++ AT    +  LE     A+ N++ L ERG  V +
Sbjct: 5   RLLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAALEGD-PVALENLKRLRERGVEVRF 64

Query: 98  GIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKK 157
           G+D   +S       + FDRI +NFPH G          +  N+EL+  F ++   +L K
Sbjct: 65  GVDCTQLSHALPADDRDFDRIYFNFPHCG------RKAGVAKNRELLAKFFQSCADVLAK 124

Query: 158 GDGEIHVT---HKEGEPYDK--------WELVEEAMKMGLKLQEAVPFRRGDYPGYQNKR 217
             GE+HVT    + G P DK        W++V  A   G  L +  PF     PGY  K 
Sbjct: 125 A-GEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGY--KC 184

Query: 218 ADGGQSDEPFRLGDCSTYKF 227
                 D PF +    TY F
Sbjct: 185 TGYRSQDRPFHIEGALTYIF 194

BLAST of MC01g1257 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.5e-15
Identity = 70/227 (30.84%), Postives = 115/227 (50.66%), Query Frame = 0

Query: 7   NEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSL-CLANRFGSARNIVA 66
           N +   ++D+  + + +   E +   +   +R LL+GEG+FSF+   L +   S   ++A
Sbjct: 28  NSQNSLTNDKIGKGKNKGPTERYVLPFEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLA 87

Query: 67  TSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHV 126
           TS DS++DL++KY +A   + ++E  G  V + IDA  +  H  L+TQ+FD I +NFPH 
Sbjct: 88  TSYDSKEDLKQKYPDAAEYISKIEINGGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHS 147

Query: 127 GFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMG 186
           G   + D    I  N++++  F +  K LL +  G I +T  E +PY  W L   A   G
Sbjct: 148 GKGIK-DQDRNILDNQKMLLAFFKASKFLLSE-KGVIVITLAETKPYTLWNLKGLAKDAG 207

Query: 187 LKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 233
                   F    YP Y ++R  G        + + S +K +L +SR
Sbjct: 208 YTSLMTEKFDSSFYPEYSHRRTIGWIDG----ISERSPWKGELRDSR 248

BLAST of MC01g1257 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-13
Identity = 56/177 (31.64%), Postives = 87/177 (49.15%), Query Frame = 0

Query: 38  RILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFY 97
           R+LLVGEG+FSF+  L+     +  + AT L    +L +    A  N++ L ERG  V +
Sbjct: 5   RLLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELARD-PLAWENLQCLRERGIDVRF 64

Query: 98  GIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKK 157
           G+D   ++  F L  + FD+I + FPH G          +  N+EL+  F ++   +L +
Sbjct: 65  GVDCTQLADVFELHEREFDQIYFIFPHCG------RKAGVAKNRELLAKFFQSCADVLAE 124

Query: 158 GDGEIHVT---HKEGEPYDK--------WELVEEAMKMGLKLQEAVPFRRGDYPGYQ 204
            +GE+HV     + G P DK        W++V  A   GL L +  PF      GY+
Sbjct: 125 -EGEVHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSDVYPFSCKAVAGYK 173

BLAST of MC01g1257 vs. NCBI nr
Match: XP_022132615.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 464 bits (1193), Expect = 1.05e-164
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0

Query: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60
           MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA
Sbjct: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60

Query: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120
           RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY
Sbjct: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120

Query: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180
           NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE
Sbjct: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180

Query: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232
           AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR
Sbjct: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232

BLAST of MC01g1257 vs. NCBI nr
Match: XP_008440954.1 (PREDICTED: uncharacterized protein At4g26485-like isoform X1 [Cucumis melo] >TYK12464.1 uncharacterized protein E5676_scaffold255G00400 [Cucumis melo var. makuwa])

HSP 1 Score: 390 bits (1001), Expect = 2.31e-135
Identity = 195/236 (82.63%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MKLATENER-----EMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLAN 60
           M+   ENER     E E  +EEEEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA 
Sbjct: 1   MEFVPENERTESSDEDEDEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAK 60

Query: 61  RFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRF 120
            FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRF
Sbjct: 61  HFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRF 120

Query: 121 DRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKW 180
           DRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+KW
Sbjct: 121 DRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYNKW 180

Query: 181 ELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           ELVEEAMK+GLKL+E VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 ELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 236

BLAST of MC01g1257 vs. NCBI nr
Match: KAA0025589.1 (uncharacterized protein E6C27_scaffold253G00790 [Cucumis melo var. makuwa])

HSP 1 Score: 389 bits (999), Expect = 4.82e-135
Identity = 197/238 (82.77%), Postives = 210/238 (88.24%), Query Frame = 0

Query: 1   MKLATENEREMESSDE-------EEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCL 60
           M+   ENER  ESSDE       EEEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCL
Sbjct: 1   MEFVPENER-TESSDEDEDEEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCL 60

Query: 61  ANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQ 120
           A  FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQ
Sbjct: 61  AKHFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQ 120

Query: 121 RFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYD 180
           RFDRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+
Sbjct: 121 RFDRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYN 180

Query: 181 KWELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           KWELVEEAMK+GLKL+E VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 KWELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 237

BLAST of MC01g1257 vs. NCBI nr
Match: XP_023003736.1 (uncharacterized protein At4g26485-like [Cucurbita maxima])

HSP 1 Score: 388 bits (996), Expect = 8.69e-135
Identity = 192/226 (84.96%), Postives = 206/226 (91.15%), Query Frame = 0

Query: 6   ENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVA 65
           ENE +MESSDE+EEEE      VWRKHYSS+HRILLVGEGDFSFSLCLAN FGSARNIVA
Sbjct: 5   ENEGKMESSDEDEEEE------VWRKHYSSKHRILLVGEGDFSFSLCLANHFGSARNIVA 64

Query: 66  TSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHV 125
           T  DSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHV
Sbjct: 65  TCRDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHV 124

Query: 126 GFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMG 185
           GFLFREDSFCQIQLNKELVEGFLRN + LLKKG+GEIHVTHKEGEPY+KWELVEEAMK G
Sbjct: 125 GFLFREDSFCQIQLNKELVEGFLRNARVLLKKGEGEIHVTHKEGEPYNKWELVEEAMKAG 184

Query: 186 LKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           L+LQE VPF + DYPGYQNKRADGGQS+ PF LGDCSTYKFKL++S
Sbjct: 185 LQLQETVPFCKNDYPGYQNKRADGGQSNAPFWLGDCSTYKFKLNQS 224

BLAST of MC01g1257 vs. NCBI nr
Match: XP_022926856.1 (uncharacterized protein At4g26485-like [Cucurbita moschata])

HSP 1 Score: 387 bits (995), Expect = 1.28e-134
Identity = 191/226 (84.51%), Postives = 207/226 (91.59%), Query Frame = 0

Query: 6   ENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVA 65
           ENE +MESSDE++EE+E     VWRKHYSS+HRILLVGEGDFSF+LCLAN FGSARNIVA
Sbjct: 5   ENEVKMESSDEDDEEDE-----VWRKHYSSKHRILLVGEGDFSFALCLANHFGSARNIVA 64

Query: 66  TSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHV 125
           T LDSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHV
Sbjct: 65  TCLDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHV 124

Query: 126 GFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMG 185
           GFLFREDSFCQIQLNKELVEGFLRN + LLKKGDGEIHVTHKEGEPY+KWELVEEAMK G
Sbjct: 125 GFLFREDSFCQIQLNKELVEGFLRNARVLLKKGDGEIHVTHKEGEPYNKWELVEEAMKAG 184

Query: 186 LKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           L+LQE VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL++S
Sbjct: 185 LQLQETVPFCKNDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNQS 225

BLAST of MC01g1257 vs. ExPASy TrEMBL
Match: A0A6J1BSY8 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111005434 PE=4 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 5.07e-165
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0

Query: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60
           MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA
Sbjct: 1   MKLATENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSA 60

Query: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120
           RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY
Sbjct: 61  RNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVY 120

Query: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180
           NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE
Sbjct: 121 NFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEE 180

Query: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232
           AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR
Sbjct: 181 AMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDESR 232

BLAST of MC01g1257 vs. ExPASy TrEMBL
Match: A0A5D3CMV7 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00400 PE=4 SV=1)

HSP 1 Score: 390 bits (1001), Expect = 1.12e-135
Identity = 195/236 (82.63%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MKLATENER-----EMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLAN 60
           M+   ENER     E E  +EEEEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA 
Sbjct: 1   MEFVPENERTESSDEDEDEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAK 60

Query: 61  RFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRF 120
            FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRF
Sbjct: 61  HFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRF 120

Query: 121 DRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKW 180
           DRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+KW
Sbjct: 121 DRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYNKW 180

Query: 181 ELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           ELVEEAMK+GLKL+E VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 ELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 236

BLAST of MC01g1257 vs. ExPASy TrEMBL
Match: A0A1S3B1V1 (uncharacterized protein At4g26485-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485215 PE=4 SV=1)

HSP 1 Score: 390 bits (1001), Expect = 1.12e-135
Identity = 195/236 (82.63%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MKLATENER-----EMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLAN 60
           M+   ENER     E E  +EEEEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCLA 
Sbjct: 1   MEFVPENERTESSDEDEDEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCLAK 60

Query: 61  RFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRF 120
            FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQRF
Sbjct: 61  HFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQRF 120

Query: 121 DRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKW 180
           DRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+KW
Sbjct: 121 DRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYNKW 180

Query: 181 ELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           ELVEEAMK+GLKL+E VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 ELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 236

BLAST of MC01g1257 vs. ExPASy TrEMBL
Match: A0A5A7SJU4 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00790 PE=4 SV=1)

HSP 1 Score: 389 bits (999), Expect = 2.33e-135
Identity = 197/238 (82.77%), Postives = 210/238 (88.24%), Query Frame = 0

Query: 1   MKLATENEREMESSDE-------EEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCL 60
           M+   ENER  ESSDE       EEEEEEEEEEE+WRKHYSSRHRILLVGEGDFSF+LCL
Sbjct: 1   MEFVPENER-TESSDEDEDEEEEEEEEEEEEEEEIWRKHYSSRHRILLVGEGDFSFALCL 60

Query: 61  ANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQ 120
           A  FGSARNIVAT LDSQDDLEKKYS+ IRNVRELEERGCL+FYGID RNMS HFFLRTQ
Sbjct: 61  AKHFGSARNIVATCLDSQDDLEKKYSDGIRNVRELEERGCLIFYGIDVRNMSTHFFLRTQ 120

Query: 121 RFDRIVYNFPHVGFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYD 180
           RFDRIVYNFPHVGFL+REDSFCQIQLNKELVEGFLRN K LLKK DGEIHV+HKEGEPY+
Sbjct: 121 RFDRIVYNFPHVGFLYREDSFCQIQLNKELVEGFLRNAKVLLKKLDGEIHVSHKEGEPYN 180

Query: 181 KWELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           KWELVEEAMK+GLKL+E VPF + DYPGYQNKRADGG S+ PF LGDCSTYKFKL+ S
Sbjct: 181 KWELVEEAMKVGLKLEETVPFHKKDYPGYQNKRADGGHSNAPFWLGDCSTYKFKLNHS 237

BLAST of MC01g1257 vs. ExPASy TrEMBL
Match: A0A6J1KQ33 (uncharacterized protein At4g26485-like OS=Cucurbita maxima OX=3661 GN=LOC111497233 PE=4 SV=1)

HSP 1 Score: 388 bits (996), Expect = 4.21e-135
Identity = 192/226 (84.96%), Postives = 206/226 (91.15%), Query Frame = 0

Query: 6   ENEREMESSDEEEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVA 65
           ENE +MESSDE+EEEE      VWRKHYSS+HRILLVGEGDFSFSLCLAN FGSARNIVA
Sbjct: 5   ENEGKMESSDEDEEEE------VWRKHYSSKHRILLVGEGDFSFSLCLANHFGSARNIVA 64

Query: 66  TSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHV 125
           T  DSQDDLE+K+S+ IRNVRELEERGCLVFYGID RNMSQHFFLRTQRFDRIVYNFPHV
Sbjct: 65  TCRDSQDDLERKFSDGIRNVRELEERGCLVFYGIDVRNMSQHFFLRTQRFDRIVYNFPHV 124

Query: 126 GFLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMG 185
           GFLFREDSFCQIQLNKELVEGFLRN + LLKKG+GEIHVTHKEGEPY+KWELVEEAMK G
Sbjct: 125 GFLFREDSFCQIQLNKELVEGFLRNARVLLKKGEGEIHVTHKEGEPYNKWELVEEAMKAG 184

Query: 186 LKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYKFKLDES 231
           L+LQE VPF + DYPGYQNKRADGGQS+ PF LGDCSTYKFKL++S
Sbjct: 185 LQLQETVPFCKNDYPGYQNKRADGGQSNAPFWLGDCSTYKFKLNQS 224

BLAST of MC01g1257 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 208.4 bits (529), Expect = 6.3e-54
Identity = 104/207 (50.24%), Postives = 142/207 (68.60%), Query Frame = 0

Query: 21  EEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSN 80
           + + +EEVW KHYSS H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS D + +KY  
Sbjct: 12  DSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKYKK 71

Query: 81  AIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQIQLN 140
           A  N++ L+  G L+ +G+DA  +  H  LR +RFDR+++NFPH GF  RE     I+ +
Sbjct: 72  ARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKH 131

Query: 141 KELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYP 200
           +ELV GF  NG + L + +GE+HV+HK   P+ +W L E A +  L L + V F + +YP
Sbjct: 132 RELVFGFF-NGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYP 191

Query: 201 GYQNKRADGGQSDEPFRLGDCSTYKFK 228
           GY+NKR DG + D+PF LG+CST+KF+
Sbjct: 192 GYENKRGDGRRCDQPFLLGECSTFKFR 217

BLAST of MC01g1257 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 165.6 bits (418), Expect = 4.7e-41
Identity = 86/189 (45.50%), Postives = 121/189 (64.02%), Query Frame = 0

Query: 31  KHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKYSNAIRNVRELEE 90
           + YS++ +ILLVGEGDFSFSL LA  FGSA NI ATSLD++++L  KY++   NV  LE 
Sbjct: 10  QQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLEL 69

Query: 91  RGCLVFYGIDARNMSQHFFLRTQRFDRIVYNFPHVGFLF-REDSFCQIQLNKELVEGFLR 150
            GC V +G++  +MS  +  R  R+DRI++NFPH G  F  E     I L++ LV GFL 
Sbjct: 70  FGCTVVHGVNVHSMSSDY--RLGRYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFLE 129

Query: 151 NGKALLKKGDGEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADG 210
           + + +LK  DGEIHVTHK  +P+++W +   A + GL+L   + F +  +PGY NK+  G
Sbjct: 130 SARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGGG 189

Query: 211 GQSDEPFRL 219
              +  F L
Sbjct: 190 SNCNSTFLL 196

BLAST of MC01g1257 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 156.0 bits (393), Expect = 3.7e-38
Identity = 90/211 (42.65%), Postives = 122/211 (57.82%), Query Frame = 0

Query: 19  EEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDSQDDLEKKY 78
           E +   +E    +HY+++ +ILLVGEGDFSFSL LA  FGSA N+ ATSLD+Q +LE+K+
Sbjct: 56  EMQTNVQETKRLRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKF 115

Query: 79  SNAIRNVRELEERGCLVFYGIDARNM-SQHFFLRTQRFDRIVYNFPHVGFLFREDSFCQI 138
            N   NV ELE  GC V YG++  +M ++     +  +DR+++NFP              
Sbjct: 116 KNGKANVEELERLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP-------------- 175

Query: 139 QLNKELVEGFLRNGKALLKKGD--GEIHVTHKEGEPYDKWELVEEAMKMGLKLQEAVPFR 198
               ELV GF+++ + L+K  D  GEIHV HK   P+ +W+L     K GL L   V F 
Sbjct: 176 --THELVRGFMKSARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFC 235

Query: 199 RGDYPGYQNKRADGGQSDEPFRLGDCSTYKF 227
              YPGY NKR  GG SD  F +G  ST+ F
Sbjct: 236 LSHYPGYFNKRGSGGYSDSSFPVGKSSTFMF 250

BLAST of MC01g1257 vs. TAIR 10
Match: AT1G55800.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 152.1 bits (383), Expect = 5.3e-37
Identity = 93/242 (38.43%), Postives = 127/242 (52.48%), Query Frame = 0

Query: 17  EEEEEEEEEEEVWRKHYSSRHRILLVGEGDFSFSLCLANRFGSARNIVATSLDS------ 76
           E   + + +EEVW KHYSS+H+ILLVGEGDFSFS  LA  FGSA NI A+SLDS      
Sbjct: 6   ELNRDRDGDEEVWVKHYSSKHQILLVGEGDFSFSCSLATCFGSASNIYASSLDSYDYKPV 65

Query: 77  ---------------------QDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFF 136
                                 DD+ +KY NA  N+  L+  G  + +G+DA  +  H  
Sbjct: 66  NKGCSFKLDFLSCCMSFMVIKPDDVVRKYKNARSNLETLKRLGAFLLHGVDATTLHFHPD 125

Query: 137 LRTQRFDRIVYNFPHVGFLFREDSFCQIQ----LNKELVEGFLRNGKALLKKGDGEIHVT 196
           LR +RFDR+++NFPH GF  +E   CQIQ      + L + FL +G + + + DGE+   
Sbjct: 126 LRYRRFDRVIFNFPHTGFHRKESDPCQIQPAAATLRNLFKDFL-HGASHMLRADGELEA- 185

Query: 197 HKEGEPYDKWELVEEAMKMGLKLQEAVPFRRGDYPGYQNKRADGGQSDEPFRLGDCSTYK 228
                                       F + +YPGY+NKR DG + D+PF LG+CST+K
Sbjct: 186 ----------------------------FEKRNYPGYENKRGDGSRCDQPFLLGECSTFK 217

BLAST of MC01g1257 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 145.2 bits (365), Expect = 6.5e-35
Identity = 79/168 (47.02%), Postives = 104/168 (61.90%), Query Frame = 0

Query: 62  NIVATSLDSQDDLEKKYSNAIRNVRELEERGCLVFYGIDARNMSQHFFLRTQRFDRIVYN 121
           NI ATSLDS+D+L  KY +A+ N+  L+  GC + + +D   MS    L  QR+DRIV+N
Sbjct: 2   NITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFN 61

Query: 122 FPHVG--FLFREDSFCQIQLNKELVEGFLRNGKALLKKGDGEIHVTHKEGEPYDKWELVE 181
           FPH G  F  RE S   I+ +KELV GFL N K +L++ DGEIH+THK   P+  W + +
Sbjct: 62  FPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEE-DGEIHITHKTTYPFSDWGIKK 121

Query: 182 EAMKMGLKLQEAVPFRRGDYPGYQNKRADGG-QSDEPFRLGDCSTYKF 227
                GLKL +   F    YPGY  KR  GG +SD+ F +G+CSTY F
Sbjct: 122 LGKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMF 168