MC01g1223 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1223 vs. ExPASy Swiss-Prot
Match: Q84J71 (Pentatricopeptide repeat-containing protein At2g17670 OS=Arabidopsis thaliana OX=3702 GN=At2g17670 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.7e-154
Identity = 278/471 (59.02%), Postives = 357/471 (75.80%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSL---SKKLPPKPSRKIQSAQSSG 60
           MGK+  SFRS +   +L +   P   APP       ++   S KLP          Q++ 
Sbjct: 1   MGKVPSSFRS-MPANLLVRKTTPSPPAPPRDFRNRTAVGGDSAKLP----------QNTQ 60

Query: 61  PLEKPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLR 120
              +P     FKS +L+DAK L++S  AT+R PLDL+F+NS+LQSY SIA +ND++   +
Sbjct: 61  APREPSLRNPFKSPNLSDAKSLFNSIAATSRIPLDLKFHNSVLQSYGSIAVVNDTVKLFQ 120

Query: 121 YMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSL 180
           ++ K QP+F P RSTF +LLS +     SS+++V ++LN MV+NG  PD+VTTDIAVRSL
Sbjct: 121 HILKSQPNFRPGRSTFLILLSHACRAPDSSISNVHRVLNLMVNNGLEPDQVTTDIAVRSL 180

Query: 181 CSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPD 240
           C  G +DEA +L+KEL+ K SPPD++TYN L+K LCK + L  VY F+DEMR  F  KPD
Sbjct: 181 CETGRVDEAKDLMKELTEKHSPPDTYTYNFLLKHLCKCKDLHVVYEFVDEMRDDFDVKPD 240

Query: 241 LVTYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYK 300
           LV++TILIDNVCN KNLREA  L+S LG  GFKPDCF+YNTIMKG+C L +GSEA+GVYK
Sbjct: 241 LVSFTILIDNVCNSKNLREAMYLVSKLGNAGFKPDCFLYNTIMKGFCTLSKGSEAVGVYK 300

Query: 301 KMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREG 360
           KMKEEG+EPD +T+NTLIFGLSK+GRV+EAR +L  M + G+ PD  TYTSLMNGMCR+G
Sbjct: 301 KMKEEGVEPDQITYNTLIFGLSKAGRVEEARMYLKTMVDAGYEPDTATYTSLMNGMCRKG 360

Query: 361 DALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYA 420
           ++LGALSLLE MEA+GC+PN CTYNTLLHGL K+RL+D+G+ELY +MKS G+KLE+  YA
Sbjct: 361 ESLGALSLLEEMEARGCAPNDCTYNTLLHGLCKARLMDKGMELYEMMKSSGVKLESNGYA 420

Query: 421 TLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQG 469
           TLVR+L +S ++AEAYEVFDYAV+SKS++D +AYSTLE+TLK LKK +EQG
Sbjct: 421 TLVRSLVKSGKVAEAYEVFDYAVDSKSLSDASAYSTLETTLKWLKKAKEQG 460

BLAST of MC01g1223 vs. ExPASy Swiss-Prot
Match: Q9ASZ8 (Pentatricopeptide repeat-containing protein At1g12620 OS=Arabidopsis thaliana OX=3702 GN=At1g12620 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 7.8e-48
Identity = 106/343 (30.90%), Postives = 180/343 (52.48%), Query Frame = 0

Query: 112 SISFLRYMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTD 171
           S++F      ++  + PD  TF  L+  +G      ++   ++++ MV  G  P  +T +
Sbjct: 124 SLAFSAMGKIIKLGYEPDTVTFSTLI--NGLCLEGRVSEALELVDRMVEMGHKPTLITLN 183

Query: 172 IAVRSLCSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSS 231
             V  LC  G + +AV L+  +      P+  TY  ++K +CKS   +     + +M   
Sbjct: 184 ALVNGLCLNGKVSDAVLLIDRMVETGFQPNEVTYGPVLKVMCKSGQTALAMELLRKMEER 243

Query: 232 FGSKPDLVTYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSE 291
              K D V Y+I+ID +C   +L  A  L + +  +GFK D  +Y T+++G+C  GR  +
Sbjct: 244 -KIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIIIYTTLIRGFCYAGRWDD 303

Query: 292 AIGVYKKMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMN 351
              + + M +  + PDVV F+ LI    K G+++EA +    M + G  PD VTYTSL++
Sbjct: 304 GAKLLRDMIKRKITPDVVAFSALIDCFVKEGKLREAEELHKEMIQRGISPDTVTYTSLID 363

Query: 352 GMCREGDALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKL 411
           G C+E     A  +L++M +KGC PN  T+N L++G  K+ L+D G+EL+  M   G+  
Sbjct: 364 GFCKENQLDKANHMLDLMVSKGCGPNIRTFNILINGYCKANLIDDGLELFRKMSLRGVVA 423

Query: 412 ETASYATLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTL 455
           +T +Y TL++  C   ++  A E+F   V  +   D+ +Y  L
Sbjct: 424 DTVTYNTLIQGFCELGKLEVAKELFQEMVSRRVRPDIVSYKIL 463

BLAST of MC01g1223 vs. ExPASy Swiss-Prot
Match: Q3EDF8 (Pentatricopeptide repeat-containing protein At1g09900 OS=Arabidopsis thaliana OX=3702 GN=At1g09900 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.0e-47
Identity = 110/380 (28.95%), Postives = 201/380 (52.89%), Query Frame = 0

Query: 92  DLRFYNSLLQSYASIATLNDSISFLRYMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASV 151
           D+  YN ++  Y     +N+++S L  M     S SPD  T++ +L +  +     L   
Sbjct: 171 DVITYNVMISGYCKAGEINNALSVLDRM-----SVSPDVVTYNTILRSLCD--SGKLKQA 230

Query: 152 QQILNFMVSNGFNPDKVTTDIAVRSLCSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQ 211
            ++L+ M+     PD +T  I + + C    +  A++L+ E+  +   PD  TYN LV  
Sbjct: 231 MEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMRDRGCTPDVVTYNVLVNG 290

Query: 212 LCKSRALSTVYGFIDEMRSSFGSKPDLVTYTILIDNVCNGKNLREATRLISVLGEEGFKP 271
           +CK   L     F+++M SS G +P+++T+ I++ ++C+     +A +L++ +  +GF P
Sbjct: 291 ICKEGRLDEAIKFLNDMPSS-GCQPNVITHNIILRSMCSTGRWMDAEKLLADMLRKGFSP 350

Query: 272 DCFVYNTIMKGYCMLGRGSEAIGVYKKMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFL 331
               +N ++   C  G    AI + +KM + G +P+ +++N L+ G  K  ++  A ++L
Sbjct: 351 SVVTFNILINFLCRKGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKEKKMDRAIEYL 410

Query: 332 DIMAEMGHFPDAVTYTSLMNGMCREGDALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKS 391
           + M   G +PD VTY +++  +C++G    A+ +L  + +KGCSP   TYNT++ GLAK+
Sbjct: 411 ERMVSRGCYPDIVTYNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITYNTVIDGLAKA 470

Query: 392 RLLDRGIELYGLMKSGGMKLETASYATLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAY 451
               + I+L   M++  +K +T +Y++LV  L R  ++ EA + F    E + M      
Sbjct: 471 GKTGKAIKLLDEMRAKDLKPDTITYSSLVGGLSREGKVDEAIKFFH---EFERMGIRPNA 530

Query: 452 STLESTLKSLKKVREQGHAI 472
            T  S +  L K R+   AI
Sbjct: 531 VTFNSIMLGLCKSRQTDRAI 539

BLAST of MC01g1223 vs. ExPASy Swiss-Prot
Match: Q9LPX2 (Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12775 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 100/329 (30.40%), Postives = 174/329 (52.89%), Query Frame = 0

Query: 126 FSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSAGLIDE 185
           + PD   F+ LL  +G      ++   ++++ MV  G  P  +T +  V  LC  G + +
Sbjct: 154 YEPDTVIFNTLL--NGLCLECRVSEALELVDRMVEMGHKPTLITLNTLVNGLCLNGKVSD 213

Query: 186 AVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVTYTILI 245
           AV L+  +      P+  TY  ++  +CKS   +     + +M      K D V Y+I+I
Sbjct: 214 AVVLIDRMVETGFQPNEVTYGPVLNVMCKSGQTALAMELLRKMEER-NIKLDAVKYSIII 273

Query: 246 DNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMKEEGLE 305
           D +C   +L  A  L + +  +GFK D   YNT++ G+C  GR  +   + + M +  + 
Sbjct: 274 DGLCKDGSLDNAFNLFNEMEIKGFKADIITYNTLIGGFCNAGRWDDGAKLLRDMIKRKIS 333

Query: 306 PDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDALGALSL 365
           P+VVTF+ LI    K G+++EA + L  M + G  P+ +TY SL++G C+E     A+ +
Sbjct: 334 PNVVTFSVLIDSFVKEGKLREADQLLKEMMQRGIAPNTITYNSLIDGFCKENRLEEAIQM 393

Query: 366 LEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLVRALCR 425
           +++M +KGC P+  T+N L++G  K+  +D G+EL+  M   G+   T +Y TLV+  C+
Sbjct: 394 VDLMISKGCDPDIMTFNILINGYCKANRIDDGLELFREMSLRGVIANTVTYNTLVQGFCQ 453

Query: 426 SDRIAEAYEVFDYAVESKSMTDVAAYSTL 455
           S ++  A ++F   V  +   D+ +Y  L
Sbjct: 454 SGKLEVAKKLFQEMVSRRVRPDIVSYKIL 479

BLAST of MC01g1223 vs. ExPASy Swiss-Prot
Match: Q9CAN0 (Pentatricopeptide repeat-containing protein At1g63130, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g63130 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.1e-45
Identity = 116/373 (31.10%), Postives = 184/373 (49.33%), Query Frame = 0

Query: 92  DLRFYNSLLQSYASIATLNDSISFLRYMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASV 151
           +L  Y+ L+  +   + L+ +++ L  M K+   + PD  T + LL  +G   G+ ++  
Sbjct: 115 NLYTYSILINCFCRRSQLSLALAVLAKMMKL--GYEPDIVTLNSLL--NGFCHGNRISDA 174

Query: 152 QQILNFMVSNGFNPDKVTTDIAVRSLCSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQ 211
             ++  MV  G+ PD  T +  +  L       EAV LV  +  K   PD  TY  +V  
Sbjct: 175 VSLVGQMVEMGYQPDSFTFNTLIHGLFRHNRASEAVALVDRMVVKGCQPDLVTYGIVVNG 234

Query: 212 LCKSRALSTVYGFIDEMRSSFGSKPDLVTYTILIDNVCNGKNLREATRLISVLGEEGFKP 271
           LCK   +      + +M      +P +V Y  +ID +CN KN+ +A  L + +  +G +P
Sbjct: 235 LCKRGDIDLALSLLKKMEQG-KIEPGVVIYNTIIDALCNYKNVNDALNLFTEMDNKGIRP 294

Query: 272 DCFVYNTIMKGYCMLGRGSEAIGVYKKMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFL 331
           +   YN++++  C  GR S+A  +   M E  + P+VVTF+ LI    K G++ EA K  
Sbjct: 295 NVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLVEAEKLY 354

Query: 332 DIMAEMGHFPDAVTYTSLMNGMCREGDALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKS 391
           D M +    PD  TY+SL+NG C       A  + E+M +K C PN  TYNTL+ G  K+
Sbjct: 355 DEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVTYNTLIKGFCKA 414

Query: 392 RLLDRGIELYGLMKSGGMKLETASYATLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAY 451
           + +D G+EL+  M   G+   T +Y TL+    ++     A  VF   V    + D+  Y
Sbjct: 415 KRVDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARECDNAQIVFKQMVSDGVLPDIMTY 474

Query: 452 STLESTLKSLKKV 465
           S L   L +  KV
Sbjct: 475 SILLDGLCNNGKV 482

BLAST of MC01g1223 vs. NCBI nr
Match: XP_022133257.1 (pentatricopeptide repeat-containing protein At2g17670 [Momordica charantia])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE
Sbjct: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS
Sbjct: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA
Sbjct: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT
Sbjct: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV
Sbjct: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI
Sbjct: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471

BLAST of MC01g1223 vs. NCBI nr
Match: XP_023003882.1 (pentatricopeptide repeat-containing protein At2g17670-like [Cucurbita maxima] >XP_023003883.1 pentatricopeptide repeat-containing protein At2g17670-like [Cucurbita maxima] >XP_023003884.1 pentatricopeptide repeat-containing protein At2g17670-like [Cucurbita maxima] >XP_023003885.1 pentatricopeptide repeat-containing protein At2g17670-like [Cucurbita maxima])

HSP 1 Score: 788 bits (2036), Expect = 3.63e-285
Identity = 409/471 (86.84%), Postives = 438/471 (92.99%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSSG  E
Sbjct: 1   MGKLSPSFRSAISTAIVNKPPNP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSGHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLA+AKKLYSSFI TT+APLD+RFYNSLL SY SIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLAEAKKLYSSFINTTKAPLDVRFYNSLLHSYTSIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQP+FSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV++GFNPDK T DIAVRSLCSA
Sbjct: 121 KVQPTFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTHGFNPDKATIDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVYGFI EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYGFIHEMRSSCGANPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL EEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPD VTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDVVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSC+YNTLLHGL+KSRLLD+GIELYGLMKSG MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCSYNTLLHGLSKSRLLDKGIELYGLMKSGDMKLEAASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVES+S+TDVAAYSTLE+TLK+LKK REQG+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESRSLTDVAAYSTLETTLKALKKAREQGNVI 470

BLAST of MC01g1223 vs. NCBI nr
Match: XP_022926982.1 (pentatricopeptide repeat-containing protein At2g17670 [Cucurbita moschata] >XP_022926983.1 pentatricopeptide repeat-containing protein At2g17670 [Cucurbita moschata] >XP_022926984.1 pentatricopeptide repeat-containing protein At2g17670 [Cucurbita moschata])

HSP 1 Score: 782 bits (2019), Expect = 1.41e-282
Identity = 409/471 (86.84%), Postives = 434/471 (92.14%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSS   E
Sbjct: 1   MGKLSPSFRSAISTPIVNKPPHP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSAHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLADAKKLYSSFI TT+APLD+RFYNSLLQSYASIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLADAKKLYSSFINTTKAPLDVRFYNSLLQSYASIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV+ GFNPDK TTDIAVRSLCSA
Sbjct: 121 KVQPSFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTQGFNPDKATTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVY FI+EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYDFIEEMRSSCGATPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL +EGFKPDCFVYN IMKGYCMLGRG EAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAKEGFKPDCFVYNIIMKGYCMLGRGVEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSCTYNTLLHGL+KSRLLDRGIELYGLMKS  MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCTYNTLLHGLSKSRLLDRGIELYGLMKSSDMKLEVASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVESKS+TDV AYSTLE TLK+LKK  E+G+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESKSLTDVTAYSTLEITLKALKKAGEKGNVI 470

BLAST of MC01g1223 vs. NCBI nr
Match: KAG7026711.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780 bits (2014), Expect = 8.13e-282
Identity = 407/471 (86.41%), Postives = 434/471 (92.14%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSS   E
Sbjct: 1   MGKLSPSFRSAISTAIVNKPPHP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSAHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLADAKKLYSSFI TT+APLD+RFYNSLLQSYASIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLADAKKLYSSFINTTKAPLDVRFYNSLLQSYASIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV+ GFNPD+ TTDIAVRSLCSA
Sbjct: 121 KVQPSFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTQGFNPDRATTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVY FI+EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYDFIEEMRSSCGATPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL +EGFKPDCFVYN IMKGYCMLGRG EAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAKEGFKPDCFVYNIIMKGYCMLGRGVEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPDAVTYTSLMNGMCR+GDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDAVTYTSLMNGMCRQGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSCTYNTLLHGL+KSRLLDRGIELYGLMKS  MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCTYNTLLHGLSKSRLLDRGIELYGLMKSSDMKLEVASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVESKS+TDV AYSTLE TLK+LKK  E+G+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESKSLTDVTAYSTLEITLKALKKAGEKGNVI 470

BLAST of MC01g1223 vs. NCBI nr
Match: KAG6594740.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 780 bits (2014), Expect = 8.13e-282
Identity = 408/471 (86.62%), Postives = 433/471 (91.93%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSS   E
Sbjct: 1   MGKLSPSFRSAISTAIVNKPPHP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSAHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLADAKKLYSSFI TT+APLD+RFYNSLLQSYASIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLADAKKLYSSFINTTKAPLDVRFYNSLLQSYASIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV+ GFNPDK TTDIAVRSLCSA
Sbjct: 121 KVQPSFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTQGFNPDKATTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVY FI+EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYDFIEEMRSSGGATPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL +EGFKPDCFVYN IMKGYCMLGRG EAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAKEGFKPDCFVYNIIMKGYCMLGRGVEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPDAVTYTSLMNGMC EGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDAVTYTSLMNGMCHEGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSCTYNTLLHGL+KSRLLDRGIELYGLMKS  MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCTYNTLLHGLSKSRLLDRGIELYGLMKSSDMKLEVASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVESKS+TDV AYSTLE TLK+LKK  E+G+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESKSLTDVTAYSTLEITLKALKKAGEKGNVI 470

BLAST of MC01g1223 vs. ExPASy TrEMBL
Match: A0A6J1BW58 (pentatricopeptide repeat-containing protein At2g17670 OS=Momordica charantia OX=3673 GN=LOC111005895 PE=4 SV=1)

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE
Sbjct: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS
Sbjct: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA
Sbjct: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT
Sbjct: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV
Sbjct: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI
Sbjct: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471

BLAST of MC01g1223 vs. ExPASy TrEMBL
Match: A0A6J1KT16 (pentatricopeptide repeat-containing protein At2g17670-like OS=Cucurbita maxima OX=3661 GN=LOC111497342 PE=4 SV=1)

HSP 1 Score: 788 bits (2036), Expect = 1.76e-285
Identity = 409/471 (86.84%), Postives = 438/471 (92.99%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSSG  E
Sbjct: 1   MGKLSPSFRSAISTAIVNKPPNP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSGHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLA+AKKLYSSFI TT+APLD+RFYNSLL SY SIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLAEAKKLYSSFINTTKAPLDVRFYNSLLHSYTSIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQP+FSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV++GFNPDK T DIAVRSLCSA
Sbjct: 121 KVQPTFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTHGFNPDKATIDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVYGFI EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYGFIHEMRSSCGANPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL EEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPD VTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDVVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSC+YNTLLHGL+KSRLLD+GIELYGLMKSG MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCSYNTLLHGLSKSRLLDKGIELYGLMKSGDMKLEAASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVES+S+TDVAAYSTLE+TLK+LKK REQG+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESRSLTDVAAYSTLETTLKALKKAREQGNVI 470

BLAST of MC01g1223 vs. ExPASy TrEMBL
Match: A0A6J1EGP9 (pentatricopeptide repeat-containing protein At2g17670 OS=Cucurbita moschata OX=3662 GN=LOC111433937 PE=4 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 6.82e-283
Identity = 409/471 (86.84%), Postives = 434/471 (92.14%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRSAIST I+NKPP P  AAP LLSGE RSLSKK PPK SR+ QSAQSS   E
Sbjct: 1   MGKLSPSFRSAISTPIVNKPPHP-PAAPSLLSGEIRSLSKKRPPKHSRENQSAQSSAHGE 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  TLFKS+SLADAKKLYSSFI TT+APLD+RFYNSLLQSYASIA+LNDSISFLR+MS
Sbjct: 61  KPKLPTLFKSASLADAKKLYSSFINTTKAPLDVRFYNSLLQSYASIASLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSP+RSTFHVLLSTSGNG+ SSLASV+QILNFMV+ GFNPDK TTDIAVRSLCSA
Sbjct: 121 KVQPSFSPERSTFHVLLSTSGNGTDSSLASVRQILNFMVTQGFNPDKATTDIAVRSLCSA 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GLIDEAVELV+E S+K SPPDS+TYNHLVKQLCKSR+LSTVY FI+EMRSS G+ PDLVT
Sbjct: 181 GLIDEAVELVREFSQKHSPPDSYTYNHLVKQLCKSRSLSTVYDFIEEMRSSCGATPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCNGKNLREATRL+SVL +EGFKPDCFVYN IMKGYCMLGRG EAIGVYKKMK
Sbjct: 241 YTILIDNVCNGKNLREATRLVSVLAKEGFKPDCFVYNIIMKGYCMLGRGVEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           EEGLEPDVVTFNTLIFGLSKSGRVK+ARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL
Sbjct: 301 EEGLEPDVVTFNTLIFGLSKSGRVKDARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLLE MEAKGCSPNSCTYNTLLHGL+KSRLLDRGIELYGLMKS  MKLE ASYAT V
Sbjct: 361 GALSLLEEMEAKGCSPNSCTYNTLLHGLSKSRLLDRGIELYGLMKSSDMKLEVASYATFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVESKS+TDV AYSTLE TLK+LKK  E+G+ I
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESKSLTDVTAYSTLEITLKALKKAGEKGNVI 470

BLAST of MC01g1223 vs. ExPASy TrEMBL
Match: A0A0A0KHF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507510 PE=4 SV=1)

HSP 1 Score: 737 bits (1903), Expect = 2.50e-265
Identity = 386/472 (81.78%), Postives = 421/472 (89.19%), Query Frame = 0

Query: 1   MGKLSPSFRSAIST-TILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPL 60
           MGKLSPSFRS +S+ T++NKPP    AAPPL        SKK  PKPSRK  S QSSG  
Sbjct: 1   MGKLSPSFRSILSSNTVINKPPHS-PAAPPL-------SSKKATPKPSRKTPSGQSSGHP 60

Query: 61  EKPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYM 120
           EKPK  T+FKS+SLADAKKLYSSF++ T+AP +LR +NSLLQSYASIATLNDSISFLR+M
Sbjct: 61  EKPKLPTVFKSASLADAKKLYSSFVSATKAPFNLRVHNSLLQSYASIATLNDSISFLRHM 120

Query: 121 SKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCS 180
           SKVQPSFSPD+STFH+LLSTSGN   S+LASVQQILNFMV+NGFNPDKVT D+AVRSLCS
Sbjct: 121 SKVQPSFSPDQSTFHILLSTSGNRPDSTLASVQQILNFMVTNGFNPDKVTADLAVRSLCS 180

Query: 181 AGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLV 240
            GL+DEAVELVKELS+K +PPD +TYNHLVKQLCKSRALSTVY FI EMRSS G+KPDLV
Sbjct: 181 VGLVDEAVELVKELSQKHTPPDIYTYNHLVKQLCKSRALSTVYNFIVEMRSSCGAKPDLV 240

Query: 241 TYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKM 300
           TYTILIDNVCN  NLREA RL+S+L +EGFKPDCFVYNTIMKGYCM+GRG+EAIGVYKKM
Sbjct: 241 TYTILIDNVCNSNNLREAMRLVSLLYKEGFKPDCFVYNTIMKGYCMVGRGAEAIGVYKKM 300

Query: 301 KEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDA 360
           KE GLEPDVVTFNTLIFGLSKSGRVKEAR FLDIMAEMGHFPDAVTYTSLMNGMCREG+A
Sbjct: 301 KEVGLEPDVVTFNTLIFGLSKSGRVKEARNFLDIMAEMGHFPDAVTYTSLMNGMCREGNA 360

Query: 361 LGALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATL 420
           LGALSLL+ MEAKGC+PNSCTYNTLLHGL+KSRLLDRGIELYGLMKS  MKLETASY+T 
Sbjct: 361 LGALSLLKEMEAKGCNPNSCTYNTLLHGLSKSRLLDRGIELYGLMKSCDMKLETASYSTF 420

Query: 421 VRALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           VRALCRS RIAEAYEVFDYAVESKS+TDV+AY +LESTLKSLK  REQ HAI
Sbjct: 421 VRALCRSGRIAEAYEVFDYAVESKSLTDVSAYLSLESTLKSLKNAREQAHAI 464

BLAST of MC01g1223 vs. ExPASy TrEMBL
Match: A0A1S4DT84 (pentatricopeptide repeat-containing protein At2g17670 OS=Cucumis melo OX=3656 GN=LOC103485180 PE=4 SV=1)

HSP 1 Score: 713 bits (1841), Expect = 6.59e-256
Identity = 375/471 (79.62%), Postives = 415/471 (88.11%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSLSKKLPPKPSRKIQSAQSSGPLE 60
           MGKLSPSFRS +ST++++KP     AAPPL        SKK  PKPSRK  S QSSG   
Sbjct: 1   MGKLSPSFRSILSTSVIHKPTLS-PAAPPL-------SSKKPTPKPSRKTPSGQSSGHPV 60

Query: 61  KPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLRYMS 120
           KPK  T+FKS+SLADAKKLYSSFI+T++AP +LR +NSLLQSYASIATLNDSISFLR+MS
Sbjct: 61  KPKLPTVFKSASLADAKKLYSSFISTSKAPFNLRVHNSLLQSYASIATLNDSISFLRHMS 120

Query: 121 KVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSA 180
           KVQPSFSPD+STFH+LLSTS N   SSLASV++ILNFMV+NGFNPDKVT D+AVRSLCS 
Sbjct: 121 KVQPSFSPDQSTFHILLSTSENRPDSSLASVRKILNFMVTNGFNPDKVTADLAVRSLCSV 180

Query: 181 GLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVT 240
           GL+DEAVELVKELS+K +P D +TYNHLVKQLCKSRALSTVY FI EMRSS G+KPDLVT
Sbjct: 181 GLVDEAVELVKELSQKHTPLDIYTYNHLVKQLCKSRALSTVYNFIVEMRSSCGAKPDLVT 240

Query: 241 YTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMK 300
           YTILIDNVCN  NLREA RL+S+L +EGFKPDCFVYNTIMKGYCM+GRG+EAIGVYKKMK
Sbjct: 241 YTILIDNVCNSNNLREAMRLVSLLYKEGFKPDCFVYNTIMKGYCMVGRGAEAIGVYKKMK 300

Query: 301 EEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDAL 360
           E GLEPD+VTFNTLIFGLSKSGRVKEA  FLDIMAEMGHFPD VTYTSLMNGMCREG+AL
Sbjct: 301 EVGLEPDLVTFNTLIFGLSKSGRVKEAIDFLDIMAEMGHFPDTVTYTSLMNGMCREGNAL 360

Query: 361 GALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLV 420
           GALSLL+ MEAKGC+PNS TYNTLLHGL+KSRLLDRGIELYGLMKS  MKLE+ASY+T V
Sbjct: 361 GALSLLKEMEAKGCNPNSFTYNTLLHGLSKSRLLDRGIELYGLMKSSDMKLESASYSTFV 420

Query: 421 RALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQGHAI 471
           RALCRS RIAEAYEVFDYAVESKS+TDV+AY +LESTLKSLK  REQ HA+
Sbjct: 421 RALCRSGRIAEAYEVFDYAVESKSLTDVSAYLSLESTLKSLKNAREQAHAV 463

BLAST of MC01g1223 vs. TAIR 10
Match: AT2G17670.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 545.8 bits (1405), Expect = 3.4e-155
Identity = 278/471 (59.02%), Postives = 357/471 (75.80%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSL---SKKLPPKPSRKIQSAQSSG 60
           MGK+  SFRS +   +L +   P   APP       ++   S KLP          Q++ 
Sbjct: 1   MGKVPSSFRS-MPANLLVRKTTPSPPAPPRDFRNRTAVGGDSAKLP----------QNTQ 60

Query: 61  PLEKPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLR 120
              +P     FKS +L+DAK L++S  AT+R PLDL+F+NS+LQSY SIA +ND++   +
Sbjct: 61  APREPSLRNPFKSPNLSDAKSLFNSIAATSRIPLDLKFHNSVLQSYGSIAVVNDTVKLFQ 120

Query: 121 YMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSL 180
           ++ K QP+F P RSTF +LLS +     SS+++V ++LN MV+NG  PD+VTTDIAVRSL
Sbjct: 121 HILKSQPNFRPGRSTFLILLSHACRAPDSSISNVHRVLNLMVNNGLEPDQVTTDIAVRSL 180

Query: 181 CSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPD 240
           C  G +DEA +L+KEL+ K SPPD++TYN L+K LCK + L  VY F+DEMR  F  KPD
Sbjct: 181 CETGRVDEAKDLMKELTEKHSPPDTYTYNFLLKHLCKCKDLHVVYEFVDEMRDDFDVKPD 240

Query: 241 LVTYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYK 300
           LV++TILIDNVCN KNLREA  L+S LG  GFKPDCF+YNTIMKG+C L +GSEA+GVYK
Sbjct: 241 LVSFTILIDNVCNSKNLREAMYLVSKLGNAGFKPDCFLYNTIMKGFCTLSKGSEAVGVYK 300

Query: 301 KMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREG 360
           KMKEEG+EPD +T+NTLIFGLSK+GRV+EAR +L  M + G+ PD  TYTSLMNGMCR+G
Sbjct: 301 KMKEEGVEPDQITYNTLIFGLSKAGRVEEARMYLKTMVDAGYEPDTATYTSLMNGMCRKG 360

Query: 361 DALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYA 420
           ++LGALSLLE MEA+GC+PN CTYNTLLHGL K+RL+D+G+ELY +MKS G+KLE+  YA
Sbjct: 361 ESLGALSLLEEMEARGCAPNDCTYNTLLHGLCKARLMDKGMELYEMMKSSGVKLESNGYA 420

Query: 421 TLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTLESTLKSLKKVREQG 469
           TLVR+L +S ++AEAYEVFDYAV+SKS++D +AYSTLE+TLK LKK +EQG
Sbjct: 421 TLVRSLVKSGKVAEAYEVFDYAVDSKSLSDASAYSTLETTLKWLKKAKEQG 460

BLAST of MC01g1223 vs. TAIR 10
Match: AT2G17670.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-107
Identity = 200/360 (55.56%), Postives = 258/360 (71.67%), Query Frame = 0

Query: 1   MGKLSPSFRSAISTTILNKPPQPLAAAPPLLSGEPRSL---SKKLPPKPSRKIQSAQSSG 60
           MGK+  SFRS +   +L +   P   APP       ++   S KLP          Q++ 
Sbjct: 1   MGKVPSSFRS-MPANLLVRKTTPSPPAPPRDFRNRTAVGGDSAKLP----------QNTQ 60

Query: 61  PLEKPKTATLFKSSSLADAKKLYSSFIATTRAPLDLRFYNSLLQSYASIATLNDSISFLR 120
              +P     FKS +L+DAK L++S  AT+R PLDL+F+NS+LQSY SIA +ND++   +
Sbjct: 61  APREPSLRNPFKSPNLSDAKSLFNSIAATSRIPLDLKFHNSVLQSYGSIAVVNDTVKLFQ 120

Query: 121 YMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSL 180
           ++ K QP+F P RSTF +LLS +     SS+++V ++LN MV+NG  PD+VTTDIAVRSL
Sbjct: 121 HILKSQPNFRPGRSTFLILLSHACRAPDSSISNVHRVLNLMVNNGLEPDQVTTDIAVRSL 180

Query: 181 CSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPD 240
           C  G +DEA +L+KEL+ K SPPD++TYN L+K LCK + L  VY F+DEMR  F  KPD
Sbjct: 181 CETGRVDEAKDLMKELTEKHSPPDTYTYNFLLKHLCKCKDLHVVYEFVDEMRDDFDVKPD 240

Query: 241 LVTYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYK 300
           LV++TILIDNVCN KNLREA  L+S LG  GFKPDCF+YNTIMKG+C L +GSEA+GVYK
Sbjct: 241 LVSFTILIDNVCNSKNLREAMYLVSKLGNAGFKPDCFLYNTIMKGFCTLSKGSEAVGVYK 300

Query: 301 KMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREG 358
           KMKEEG+EPD +T+NTLIFGLSK+GRV+EAR +L  M + G+ PD  TYTSLMNGMCR+G
Sbjct: 301 KMKEEGVEPDQITYNTLIFGLSKAGRVEEARMYLKTMVDAGYEPDTATYTSLMNGMCRKG 349

BLAST of MC01g1223 vs. TAIR 10
Match: AT1G12620.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-49
Identity = 106/343 (30.90%), Postives = 180/343 (52.48%), Query Frame = 0

Query: 112 SISFLRYMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTD 171
           S++F      ++  + PD  TF  L+  +G      ++   ++++ MV  G  P  +T +
Sbjct: 124 SLAFSAMGKIIKLGYEPDTVTFSTLI--NGLCLEGRVSEALELVDRMVEMGHKPTLITLN 183

Query: 172 IAVRSLCSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSS 231
             V  LC  G + +AV L+  +      P+  TY  ++K +CKS   +     + +M   
Sbjct: 184 ALVNGLCLNGKVSDAVLLIDRMVETGFQPNEVTYGPVLKVMCKSGQTALAMELLRKMEER 243

Query: 232 FGSKPDLVTYTILIDNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSE 291
              K D V Y+I+ID +C   +L  A  L + +  +GFK D  +Y T+++G+C  GR  +
Sbjct: 244 -KIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIIIYTTLIRGFCYAGRWDD 303

Query: 292 AIGVYKKMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMN 351
              + + M +  + PDVV F+ LI    K G+++EA +    M + G  PD VTYTSL++
Sbjct: 304 GAKLLRDMIKRKITPDVVAFSALIDCFVKEGKLREAEELHKEMIQRGISPDTVTYTSLID 363

Query: 352 GMCREGDALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKL 411
           G C+E     A  +L++M +KGC PN  T+N L++G  K+ L+D G+EL+  M   G+  
Sbjct: 364 GFCKENQLDKANHMLDLMVSKGCGPNIRTFNILINGYCKANLIDDGLELFRKMSLRGVVA 423

Query: 412 ETASYATLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAYSTL 455
           +T +Y TL++  C   ++  A E+F   V  +   D+ +Y  L
Sbjct: 424 DTVTYNTLIQGFCELGKLEVAKELFQEMVSRRVRPDIVSYKIL 463

BLAST of MC01g1223 vs. TAIR 10
Match: AT1G09900.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-48
Identity = 110/380 (28.95%), Postives = 201/380 (52.89%), Query Frame = 0

Query: 92  DLRFYNSLLQSYASIATLNDSISFLRYMSKVQPSFSPDRSTFHVLLSTSGNGSGSSLASV 151
           D+  YN ++  Y     +N+++S L  M     S SPD  T++ +L +  +     L   
Sbjct: 171 DVITYNVMISGYCKAGEINNALSVLDRM-----SVSPDVVTYNTILRSLCD--SGKLKQA 230

Query: 152 QQILNFMVSNGFNPDKVTTDIAVRSLCSAGLIDEAVELVKELSRKQSPPDSFTYNHLVKQ 211
            ++L+ M+     PD +T  I + + C    +  A++L+ E+  +   PD  TYN LV  
Sbjct: 231 MEVLDRMLQRDCYPDVITYTILIEATCRDSGVGHAMKLLDEMRDRGCTPDVVTYNVLVNG 290

Query: 212 LCKSRALSTVYGFIDEMRSSFGSKPDLVTYTILIDNVCNGKNLREATRLISVLGEEGFKP 271
           +CK   L     F+++M SS G +P+++T+ I++ ++C+     +A +L++ +  +GF P
Sbjct: 291 ICKEGRLDEAIKFLNDMPSS-GCQPNVITHNIILRSMCSTGRWMDAEKLLADMLRKGFSP 350

Query: 272 DCFVYNTIMKGYCMLGRGSEAIGVYKKMKEEGLEPDVVTFNTLIFGLSKSGRVKEARKFL 331
               +N ++   C  G    AI + +KM + G +P+ +++N L+ G  K  ++  A ++L
Sbjct: 351 SVVTFNILINFLCRKGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKEKKMDRAIEYL 410

Query: 332 DIMAEMGHFPDAVTYTSLMNGMCREGDALGALSLLEVMEAKGCSPNSCTYNTLLHGLAKS 391
           + M   G +PD VTY +++  +C++G    A+ +L  + +KGCSP   TYNT++ GLAK+
Sbjct: 411 ERMVSRGCYPDIVTYNTMLTALCKDGKVEDAVEILNQLSSKGCSPVLITYNTVIDGLAKA 470

Query: 392 RLLDRGIELYGLMKSGGMKLETASYATLVRALCRSDRIAEAYEVFDYAVESKSMTDVAAY 451
               + I+L   M++  +K +T +Y++LV  L R  ++ EA + F    E + M      
Sbjct: 471 GKTGKAIKLLDEMRAKDLKPDTITYSSLVGGLSREGKVDEAIKFFH---EFERMGIRPNA 530

Query: 452 STLESTLKSLKKVREQGHAI 472
            T  S +  L K R+   AI
Sbjct: 531 VTFNSIMLGLCKSRQTDRAI 539

BLAST of MC01g1223 vs. TAIR 10
Match: AT1G12775.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 8.9e-47
Identity = 100/329 (30.40%), Postives = 174/329 (52.89%), Query Frame = 0

Query: 126 FSPDRSTFHVLLSTSGNGSGSSLASVQQILNFMVSNGFNPDKVTTDIAVRSLCSAGLIDE 185
           + PD   F+ LL  +G      ++   ++++ MV  G  P  +T +  V  LC  G + +
Sbjct: 154 YEPDTVIFNTLL--NGLCLECRVSEALELVDRMVEMGHKPTLITLNTLVNGLCLNGKVSD 213

Query: 186 AVELVKELSRKQSPPDSFTYNHLVKQLCKSRALSTVYGFIDEMRSSFGSKPDLVTYTILI 245
           AV L+  +      P+  TY  ++  +CKS   +     + +M      K D V Y+I+I
Sbjct: 214 AVVLIDRMVETGFQPNEVTYGPVLNVMCKSGQTALAMELLRKMEER-NIKLDAVKYSIII 273

Query: 246 DNVCNGKNLREATRLISVLGEEGFKPDCFVYNTIMKGYCMLGRGSEAIGVYKKMKEEGLE 305
           D +C   +L  A  L + +  +GFK D   YNT++ G+C  GR  +   + + M +  + 
Sbjct: 274 DGLCKDGSLDNAFNLFNEMEIKGFKADIITYNTLIGGFCNAGRWDDGAKLLRDMIKRKIS 333

Query: 306 PDVVTFNTLIFGLSKSGRVKEARKFLDIMAEMGHFPDAVTYTSLMNGMCREGDALGALSL 365
           P+VVTF+ LI    K G+++EA + L  M + G  P+ +TY SL++G C+E     A+ +
Sbjct: 334 PNVVTFSVLIDSFVKEGKLREADQLLKEMMQRGIAPNTITYNSLIDGFCKENRLEEAIQM 393

Query: 366 LEVMEAKGCSPNSCTYNTLLHGLAKSRLLDRGIELYGLMKSGGMKLETASYATLVRALCR 425
           +++M +KGC P+  T+N L++G  K+  +D G+EL+  M   G+   T +Y TLV+  C+
Sbjct: 394 VDLMISKGCDPDIMTFNILINGYCKANRIDDGLELFREMSLRGVIANTVTYNTLVQGFCQ 453

Query: 426 SDRIAEAYEVFDYAVESKSMTDVAAYSTL 455
           S ++  A ++F   V  +   D+ +Y  L
Sbjct: 454 SGKLEVAKKLFQEMVSRRVRPDIVSYKIL 479