MC01g0721 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0721 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.6e-51
Identity = 145/429 (33.80%), Postives = 220/429 (51.28%), Query Frame = 0

Query: 85  STSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEES----LTDLLLAAAEAV--EAQNQ 144
           +T+TD+   EM++              I+   + ++S    L  LL+A AEA+    +N+
Sbjct: 82  TTTTDEEEEEMETTTTTTT------TAIKTHEVGDDSKGLKLVHLLMAGAEALTGSTKNR 141

Query: 145 PLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGL-------------HYKSTLTNFFPPR 204
            L  V++ +LK L+   +  S   RLA  FT+ L             H+       +   
Sbjct: 142 DLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAHNNHHHHNNNKHYLTT 201

Query: 205 EMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQWPP 264
                 + ++++AFQ+LQ++SPY+KF HFTANQAI+EA   E  +HVID+DIMEG+QW  
Sbjct: 202 NGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWAS 261

Query: 265 LMADLAAKKDFSLLRLTAVVQ---DHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKA 324
           L+  LA+  +   LR+TA+ +      +   +++TGRRL+ FA S+  PF F H R++  
Sbjct: 262 LIQSLASNNNGPHLRITALSRTGTGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDSD 321

Query: 325 ENFG----KIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFK 384
           E F     K+  G+A++ NC   L H LS+     + +FL+GA  L+PK V LVEEE+  
Sbjct: 322 ETFRPSALKLVRGEALVFNCMLNLPH-LSYRAPESVASFLNGAKTLNPKLVTLVEEEVGS 381

Query: 385 VSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCD 444
           V       FVE F ++ HH+SA+ DSL   F     +       F G RI  S+ +    
Sbjct: 382 VIG----GFVERFMDSLHHYSAVFDSLEAGFPMQNRARTLVERVFFGPRIAGSLGRIYRT 441

Query: 445 DNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQH---EKCRLSLCWKS 485
                  S G      G++ +P S  N CQAK L+ LF   + V+       +L L WKS
Sbjct: 442 GGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKS 499

BLAST of MC01g0721 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.2e-45
Identity = 148/474 (31.22%), Postives = 238/474 (50.21%), Query Frame = 0

Query: 35  GNSSCYSHSENNSSQNSLQDGSFLEYGDQDLIFLEEALEFDPTNYNPDFLSTSTDQNPAE 94
           GN   + H  ++   ++    +F ++        EE  E   T       +T+T    A+
Sbjct: 48  GNQDDFHHLIDSMIDDNNTGPAFSDHTASTTSEEEEEEEATTTTMTTTTTTTTTTPEAAD 107

Query: 95  MDSWIQPNEKDGSFFEGIQAELMEEESLTDLLLAAAEAVEA--QNQPLVSVLIEKLKNLL 154
            D            F+G++        L  LL+A AEA+    +N+ L  V++ +LK L+
Sbjct: 108 DD------------FKGLR--------LVHLLMAGAEALTGANKNRELARVILVRLKELV 167

Query: 155 LCDSGSSPFNRLAWFFTQGLH---------YKSTLTNFF---PPREMADQEKKNSISAFQ 214
               G++   RLA +FT+ L          Y S+  +     P  E     + ++++AFQ
Sbjct: 168 SHTDGTN-MERLAAYFTEALQGLLEGAGGAYNSSSKHHVIGGPHHE----PQNDALAAFQ 227

Query: 215 MLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQWPPLMADLAAKKDFSLLR 274
           +LQ++SPY+KF HFTANQAI+EA   E  +H++D+DIMEG+QW  LM  LA+  +   LR
Sbjct: 228 LLQDMSPYVKFGHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQALASNPNGPHLR 287

Query: 275 LTAVVQD---HENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAENFG----KIQVGQA 334
           +TA+ +      +   +++TGRRL+ FA S+  PF F H R+E  E F     K+  G+A
Sbjct: 288 ITALSRSGVGRRSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEA 347

Query: 335 VIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQPMSFVEFFFE 394
           ++ NC   L H L++ + + + +FL+ A  L P+ V +VEEE+          FVE F +
Sbjct: 348 LVFNCMLNLPH-LTYRSPNSVASFLTAAKALRPRLVTVVEEEVGSALG----GFVERFMD 407

Query: 395 AFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRNLLSNGFDHYL 454
           + HHFSA+ DSL   F     +       FLG RI+ S+ +              +  +L
Sbjct: 408 SLHHFSAVFDSLEAGFPMQGRARALVERVFLGPRIVGSLARI-YRTGGGGEERGSWREWL 467

Query: 455 K--GYKKIPFSSFNRCQAKYLVSLFRGDFWVQH-EKCRLSLCWKSRPLCTATIW 485
           +  G+  +  SS N CQ+  L+ LF   + V+     +L L WK+R L +A++W
Sbjct: 468 RAAGFSGVAVSSANHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRLLSASLW 490

BLAST of MC01g0721 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.9e-43
Identity = 137/413 (33.17%), Postives = 207/413 (50.12%), Query Frame = 0

Query: 122 LTDLLLAAAEAVEA--QNQPLVSVLIEKLKNLL----LCDSGSSPFNRLAWFFTQGLHYK 181
           L  LL+AAAEA+    +++ L  V++ +LK ++      ++ +S   RLA  FT  L  +
Sbjct: 132 LLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERLAAHFTDAL--Q 191

Query: 182 STLTNFFP--------PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGE 241
             L    P            +     + ++AFQMLQ++SPY+KF HFTANQAILEA  G+
Sbjct: 192 GLLDGSHPVGGSGRQAAAAASHHHAGDVLTAFQMLQDMSPYMKFGHFTANQAILEAVSGD 251

Query: 242 EALHVIDFDIMEGIQWPPLMADLAAKKD---FSLLRLTAVVQDHENETK-IEQTGRRLSE 301
             +H++D+DI EGIQW  LM  + ++ D      LR+TAV +      + +++ GRRLS 
Sbjct: 252 RRVHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRSGGGGARAVQEAGRRLSA 311

Query: 302 FAKSINFPFEFDHMRIEKAENFGKIQV----GQAVIANCSGILHHILSHSNISK----LE 361
           FA SI  PF F   R++  E F    V    G+A++ANC  +LH   + + I +    + 
Sbjct: 312 FAASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVANC--VLHQAAATTTIRRPTGSVA 371

Query: 362 TFLSGAAKLSPKCVVLVEEELFKVSKPQPMS--------FVEFFFEAFHHFSALSDSLLR 421
           +FLSG A L  K V +VEEE          S        FV  F E  H +SA+ DSL  
Sbjct: 372 SFLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGFVRQFMEELHRYSAVWDSL-- 431

Query: 422 CFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRNLLSN-----------GFDHYLK-- 481
                 E+GF       G  ++E V   P   N+   +S            G+  +++  
Sbjct: 432 ------EAGFPTQSRVRG--LVERVILAP---NIAGAVSRAYRGVDGEGRCGWGQWMRGS 491

Query: 482 GYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE-KCRLSLCWKSRPLCTATIWVP 487
           G+  +P S FN  QA+ L+ LF   + V+     ++ L WK+R L +A++W P
Sbjct: 492 GFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVWAP 527

BLAST of MC01g0721 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 7.4e-41
Identity = 156/493 (31.64%), Postives = 245/493 (49.70%), Query Frame = 0

Query: 18  FPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQ---DGSFLE-----YGDQDLIFLE 77
           +P E F+    F+ H+   ++  S S N  S N L    D  F +      GD+  + +E
Sbjct: 3   YPYEDFLDLF-FSTHTDPLATAASTSSNGYSLNDLDIDWDCDFRDVIESIMGDEGAM-ME 62

Query: 78  EALEFDPTNYNPDFL--STSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLTDLLL 137
              E  P  ++ + L  S ST  + A+  S+ +P   +    +G++        L  LL+
Sbjct: 63  PESEAVPMLHDQEGLCNSASTGLSVADGVSFGEPKTDES---KGLR--------LVHLLV 122

Query: 138 AAAEAVEAQN--QPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFPPR 197
           AAA+A    N  + L  V++ +LK+ L+     +   RLA  FT GL       +   P+
Sbjct: 123 AAADASTGANKSRELTRVILARLKD-LVSPGDRTNMERLAAHFTNGLSKLLERDSVLCPQ 182

Query: 198 EMADQ--EKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 257
           +  D   ++ + ISAF++LQ +SPY+ F + TA QAILEA + E  +H++D+DI EG+QW
Sbjct: 183 QHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDINEGVQW 242

Query: 258 PPLMADLAAKK---DFSLLRLTAV---VQDHENETKIEQTGRRLSEFAKSINFPFEFDHM 317
             LM  L ++        LR+TA+       ++   +++TGRRL+ FA SI  PF + H 
Sbjct: 243 ASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIGQPFSYQHC 302

Query: 318 RIEK---AENFGKIQVGQAVIANCSGILH-HILSHSNISKLETFLSGAAKLSPKCVVLVE 377
           +++    + +  K+  G+AV+ NC  +LH    SH   S + +FLS A  L+PK V LV 
Sbjct: 303 KLDTNAFSTSSLKLVRGEAVVINC--MLHLPRFSHQTPSSVISFLSEAKTLNPKLVTLVH 362

Query: 378 EELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVR 437
           EE+  +       F+  F +  H FSA+ DSL      A  +       F+G  +   + 
Sbjct: 363 EEVGLMGN---QGFLYRFMDLLHQFSAIFDSLEAGLSIANPARGFVERVFIGPWVANWLT 422

Query: 438 QFPCDD-NVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQH-EKCRLSL 485
           +   +D  V +  S        G+K +  S  NRCQAK L+SLF   F V+   +  L L
Sbjct: 423 RITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFRVEELGQNGLVL 476

BLAST of MC01g0721 vs. ExPASy Swiss-Prot
Match: Q9M384 (Protein SCARECROW OS=Arabidopsis thaliana OX=3702 GN=SCR PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.5e-33
Identity = 132/408 (32.35%), Postives = 197/408 (48.28%), Query Frame = 0

Query: 110 EGIQAELMEEES--LTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPF----NR 169
           E I+ +  +EE   L  LLL  AEAV A N      L E  K LL     S+P+     R
Sbjct: 276 EEIKRQKQDEEGLHLLTLLLQCAEAVSADN------LEEANKLLLEISQLSTPYGTSAQR 335

Query: 170 LAWFFTQGLHYK---STLTNF--FPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQ 229
           +A +F++ +  +   S L  +   P R M        +SAFQ+   +SP +KF+HFTANQ
Sbjct: 336 VAAYFSEAMSARLLNSCLGIYAALPSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQ 395

Query: 230 AILEAAQGEEALHVIDFDIMEGIQWPPLMADLAAKKDF-SLLRLTAVVQDHENETKIEQT 289
           AI EA + E+++H+ID DIM+G+QWP L   LA++      +RLT +    E    ++ T
Sbjct: 396 AIQEAFEKEDSVHIIDLDIMQGLQWPGLFHILASRPGGPPHVRLTGLGTSME---ALQAT 455

Query: 290 GRRLSEFAKSINFPFEFDHMRIEKAENFGKIQVGQAVIANCSGILHHILSHS--NISKLE 349
           G+RLS+FA  +  PFEF  +    AE  G +   +  +     +  H L HS  +++  +
Sbjct: 456 GKRLSDFADKLGLPFEFCPL----AEKVGNLDTERLNVRKREAVAVHWLQHSLYDVTGSD 515

Query: 350 TF-LSGAAKLSPKCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYE 409
              L    +L+PK V +VE++L         SF+  F EA H++SAL DSL   +    E
Sbjct: 516 AHTLWLLQRLAPKVVTVVEQDLSHAG-----SFLGRFVEAIHYYSALFDSLGASY--GEE 575

Query: 410 SGFRQVEEFLGARILESVRQFPCDDNVRNLLSNG---------FDHYLK-----GYKKIP 469
           S  R V           V Q      +RN+L+ G         F+ + +     G+K I 
Sbjct: 576 SEERHV-----------VEQQLLSKEIRNVLAVGGPSRSGEVKFESWREKMQQCGFKGIS 635

Query: 470 FSSFNRCQAKYLVSLFRGD-FWVQHEKCRLSLCWKSRPLCTATIWVPR 488
            +     QA  L+ +F  D + +  +   L L WK   L TA+ W PR
Sbjct: 636 LAGNAATQATLLLGMFPSDGYTLVDDNGTLKLGWKDLSLLTASAWTPR 652

BLAST of MC01g0721 vs. NCBI nr
Match: XP_022133188.1 (nodulation-signaling pathway 2 protein-like [Momordica charantia])

HSP 1 Score: 984 bits (2543), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 4   MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ 63
           MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ
Sbjct: 1   MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ 60

Query: 64  DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT 123
           DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT
Sbjct: 61  DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT 120

Query: 124 DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP 183
           DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP
Sbjct: 121 DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP 180

Query: 184 PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 243
           PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW
Sbjct: 181 PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 240

Query: 244 PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE 303
           PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE
Sbjct: 241 PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE 300

Query: 304 NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ 363
           NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ
Sbjct: 301 NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ 360

Query: 364 PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN 423
           PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN
Sbjct: 361 PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN 420

Query: 424 LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI 483
           LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI
Sbjct: 421 LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI 480

Query: 484 WVPRVKSGAK 493
           WVPRVKSGAK
Sbjct: 481 WVPRVKSGAK 490

BLAST of MC01g0721 vs. NCBI nr
Match: XP_023518115.1 (nodulation-signaling pathway 2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645 bits (1665), Expect = 6.01e-228
Identity = 364/504 (72.22%), Postives = 409/504 (81.15%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEY 60
           +N+MELEPI+FLTNYSYF      Q+HEF E S  NS C S SEN SSQNSLQDG FLEY
Sbjct: 1   MNTMELEPIDFLTNYSYFSQ----QSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEY 60

Query: 61  GDQDLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEM-DSW-IQPNEKDG-----SFFEG 120
            DQ+L+  +E  +FDP NYN  +FL+T+ D QN  EM DS  +Q +E++      + F+G
Sbjct: 61  ADQNLL-QQELDQFDPINYNFEEFLATTMDHQNIPEMGDSRSVQEDEEEEEGNSKTMFKG 120

Query: 121 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQG 180
           IQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS FN+LAWFFTQG
Sbjct: 121 IQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQG 180

Query: 181 LHYKSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEE 240
           LHYK T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+
Sbjct: 181 LHYK-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEK 240

Query: 241 ALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKS 300
            +HVIDFDIMEGIQWPPLMADLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKS
Sbjct: 241 MIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKS 300

Query: 301 INFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSP 360
           IN PF+FDHM IEK ENF +IQV GQ VIANCSGILH  LSH N SKLETFL+G AKLSP
Sbjct: 301 INLPFKFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSP 360

Query: 361 KCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLG 420
           KCVVLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G YE+GF+QV +EFLG
Sbjct: 361 KCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLG 420

Query: 421 ARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE 480
            RILESV QFPCD N  NL    FD YLKGYKKIPFSSFN  QAKYL++LFRGDFWVQHE
Sbjct: 421 TRILESVSQFPCDKNAMNLREKAFD-YLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHE 480

Query: 481 KCRLSLCWKSRPLCTATIWVPRVK 489
              LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 NGSLSLCWKSRPLCSATIWVPTAK 494

BLAST of MC01g0721 vs. NCBI nr
Match: XP_022962651.1 (nodulation-signaling pathway 2 protein-like [Cucurbita moschata])

HSP 1 Score: 642 bits (1657), Expect = 9.88e-227
Identity = 359/504 (71.23%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEY 60
           +N+MELEPI+FLTNYSYF      Q+HEF E S  NS C S SEN SSQNSLQDG FLEY
Sbjct: 1   MNTMELEPIDFLTNYSYFSQ----QSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEY 60

Query: 61  GDQDLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEM-------DSWIQPNEKDGSFFEG 120
            DQ+L+  +E  +FDP NYN  DFL+T+ D Q+  EM       +   +  E   + F+G
Sbjct: 61  ADQNLL-QQELDQFDPINYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKG 120

Query: 121 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQG 180
           IQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS FN+LAWFFTQG
Sbjct: 121 IQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQG 180

Query: 181 LHYKSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEE 240
           LHYK T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+
Sbjct: 181 LHYK-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEK 240

Query: 241 ALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKS 300
            +HVIDFDIMEGIQWPPLMADLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKS
Sbjct: 241 MIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKS 300

Query: 301 INFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSP 360
           IN PF+FDHM IEK ENF +IQV GQ VIANCSGILH  LSH N SKLETFL+G AKLSP
Sbjct: 301 INLPFKFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSP 360

Query: 361 KCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLG 420
           KCVVLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G YE+GF+QV +EFLG
Sbjct: 361 KCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLG 420

Query: 421 ARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE 480
            RILESV QFPC+ N  NL    FD Y KGYKKIPFSSFN  QAKYL++LFRGDFWVQHE
Sbjct: 421 TRILESVSQFPCEKNAMNLREKAFD-YFKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHE 480

Query: 481 KCRLSLCWKSRPLCTATIWVPRVK 489
              LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 NGSLSLCWKSRPLCSATIWVPTAK 494

BLAST of MC01g0721 vs. NCBI nr
Match: KAG6595119.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 640 bits (1652), Expect = 5.10e-226
Identity = 359/501 (71.66%), Postives = 402/501 (80.24%), Query Frame = 0

Query: 4   MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ 63
           MELEPI+FLTNYSYF      Q+HEF E S  NS C S SEN SSQNSLQDG FLEY DQ
Sbjct: 1   MELEPIDFLTNYSYFSQ----QSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEYADQ 60

Query: 64  DLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEM-------DSWIQPNEKDGSFFEGIQA 123
           +L+  +E  +FDP NYN  DFL+T+ D Q+  EM       +   +  E   + F+GIQA
Sbjct: 61  NLL-QQELDQFDPINYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKGIQA 120

Query: 124 ELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHY 183
           ELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS FN+LAWFFT+GLHY
Sbjct: 121 ELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTRGLHY 180

Query: 184 KSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALH 243
           K T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+ +H
Sbjct: 181 K-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIH 240

Query: 244 VIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKSINF 303
           VIDFDIMEGIQWPPLMADLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKSIN 
Sbjct: 241 VIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINL 300

Query: 304 PFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCV 363
           PF+FDHM IEK ENF +IQV GQAVIANCSGILH  LSH N SKLETFL+G AKLSPKCV
Sbjct: 301 PFKFDHMGIEKDENFEQIQVLGQAVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCV 360

Query: 364 VLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLGARI 423
           VLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G YE+GF+QV +EFLG RI
Sbjct: 361 VLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRI 420

Query: 424 LESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCR 483
           LESV QFPC+ N  NL    FD YLKGYKKIPFSSFN  QAKYL++LFRGDFWVQHE   
Sbjct: 421 LESVSQFPCEKNAMNLREKAFD-YLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGS 480

Query: 484 LSLCWKSRPLCTATIWVPRVK 489
           LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 LSLCWKSRPLCSATIWVPTAK 491

BLAST of MC01g0721 vs. NCBI nr
Match: KAG7027132.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640 bits (1652), Expect = 5.69e-226
Identity = 358/504 (71.03%), Postives = 404/504 (80.16%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEY 60
           +N+MELEPI+FLTNYSYF      Q+HEF E S  NS C S SEN SSQNSLQDG FLEY
Sbjct: 1   MNTMELEPIDFLTNYSYFSQ----QSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEY 60

Query: 61  GDQDLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEM-------DSWIQPNEKDGSFFEG 120
            DQ+L+  +E  +FDP NYN  DFL+T+ D Q+  EM       +   +  E   + F+G
Sbjct: 61  ADQNLL-QQELDQFDPINYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKG 120

Query: 121 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQG 180
           IQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS F++LAWFFT+G
Sbjct: 121 IQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFSQLAWFFTRG 180

Query: 181 LHYKSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEE 240
           LHYK T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+
Sbjct: 181 LHYK-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEK 240

Query: 241 ALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKS 300
            +HVIDFDIMEGIQWPPLMADLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKS
Sbjct: 241 MIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKS 300

Query: 301 INFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSP 360
           IN PF+FDHM IEK ENF +IQV GQ VIANCSGILH  LSH N SKLETFL+G AKLSP
Sbjct: 301 INLPFKFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSP 360

Query: 361 KCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLG 420
           KCVVLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G YE+GF+QV +EFLG
Sbjct: 361 KCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLG 420

Query: 421 ARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE 480
            RILESV QFPC+ N  NL    FD YLKGYKKIPFSSFN  QAKYL++LFRGDFWVQHE
Sbjct: 421 TRILESVSQFPCEKNAMNLREKAFD-YLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHE 480

Query: 481 KCRLSLCWKSRPLCTATIWVPRVK 489
              LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 NGSLSLCWKSRPLCSATIWVPTAK 494

BLAST of MC01g0721 vs. ExPASy TrEMBL
Match: A0A6J1BYD4 (nodulation-signaling pathway 2 protein-like OS=Momordica charantia OX=3673 GN=LOC111005848 PE=3 SV=1)

HSP 1 Score: 984 bits (2543), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 4   MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ 63
           MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ
Sbjct: 1   MELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEYGDQ 60

Query: 64  DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT 123
           DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT
Sbjct: 61  DLIFLEEALEFDPTNYNPDFLSTSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLT 120

Query: 124 DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP 183
           DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP
Sbjct: 121 DLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFP 180

Query: 184 PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 243
           PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW
Sbjct: 181 PREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 240

Query: 244 PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE 303
           PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE
Sbjct: 241 PPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHMRIEKAE 300

Query: 304 NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ 363
           NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ
Sbjct: 301 NFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVEEELFKVSKPQ 360

Query: 364 PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN 423
           PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN
Sbjct: 361 PMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVRQFPCDDNVRN 420

Query: 424 LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI 483
           LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI
Sbjct: 421 LLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHEKCRLSLCWKSRPLCTATI 480

Query: 484 WVPRVKSGAK 493
           WVPRVKSGAK
Sbjct: 481 WVPRVKSGAK 490

BLAST of MC01g0721 vs. ExPASy TrEMBL
Match: A0A6J1HDU3 (nodulation-signaling pathway 2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463069 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 4.79e-227
Identity = 359/504 (71.23%), Postives = 403/504 (79.96%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEY 60
           +N+MELEPI+FLTNYSYF      Q+HEF E S  NS C S SEN SSQNSLQDG FLEY
Sbjct: 1   MNTMELEPIDFLTNYSYFSQ----QSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEY 60

Query: 61  GDQDLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEM-------DSWIQPNEKDGSFFEG 120
            DQ+L+  +E  +FDP NYN  DFL+T+ D Q+  EM       +   +  E   + F+G
Sbjct: 61  ADQNLL-QQELDQFDPINYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKG 120

Query: 121 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQG 180
           IQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS FN+LAWFFTQG
Sbjct: 121 IQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQG 180

Query: 181 LHYKSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEE 240
           LHYK T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+
Sbjct: 181 LHYK-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEK 240

Query: 241 ALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKS 300
            +HVIDFDIMEGIQWPPLMADLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKS
Sbjct: 241 MIHVIDFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKS 300

Query: 301 INFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSP 360
           IN PF+FDHM IEK ENF +IQV GQ VIANCSGILH  LSH N SKLETFL+G AKLSP
Sbjct: 301 INLPFKFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSP 360

Query: 361 KCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLG 420
           KCVVLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G YE+GF+QV +EFLG
Sbjct: 361 KCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLG 420

Query: 421 ARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE 480
            RILESV QFPC+ N  NL    FD Y KGYKKIPFSSFN  QAKYL++LFRGDFWVQHE
Sbjct: 421 TRILESVSQFPCEKNAMNLREKAFD-YFKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHE 480

Query: 481 KCRLSLCWKSRPLCTATIWVPRVK 489
              LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 NGSLSLCWKSRPLCSATIWVPTAK 494

BLAST of MC01g0721 vs. ExPASy TrEMBL
Match: A0A6J1KQT9 (nodulation-signaling pathway 2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111497427 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 2.25e-225
Identity = 358/504 (71.03%), Postives = 402/504 (79.76%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLEY 60
           +N+MELEPI+FLTNYSYF    F Q+HEF E S  NS C S SEN SSQNSLQDG FLEY
Sbjct: 1   MNTMELEPIDFLTNYSYF----FQQSHEFGECSLTNS-CSSLSEN-SSQNSLQDGYFLEY 60

Query: 61  GDQDLIFLEEALEFDPTNYN-PDFLSTSTD-QNPAEMDSWIQPNEKDG-------SFFEG 120
            DQ+L+  +E  +FDP NYN  DFL+T+ D Q+  EM       E+D        + F+G
Sbjct: 61  ADQNLL-QQELDQFDPNNYNFEDFLTTTMDHQSIPEMGDSRSVQEEDEEEEENSKTMFKG 120

Query: 121 IQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQG 180
           IQAELMEEESLTDLLLAAA+AV+AQN PLVS LI+KL NLLLCD GSS FN+LAWFFTQG
Sbjct: 121 IQAELMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQG 180

Query: 181 LHYKSTLTNFFPPREMADQEK---KNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEE 240
           LHYK T+       EMA+Q++   KNS+SAF MLQ+LSPYIKFAHFTANQAILEA++GE+
Sbjct: 181 LHYK-TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEK 240

Query: 241 ALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETK-IEQTGRRLSEFAKS 300
            +HVIDFDIMEGIQWPPLM DLAAKK   LLRLTA+ Q +ENE + IE+TGRRLSEFAKS
Sbjct: 241 MIHVIDFDIMEGIQWPPLMEDLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKS 300

Query: 301 INFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSP 360
           IN PF+FDHM IEK ENF +IQV GQ VI NCSGILH  LSH N SKLETFL+G +KLSP
Sbjct: 301 INLPFKFDHMGIEKDENFEQIQVLGQTVIGNCSGILHS-LSHRNSSKLETFLNGISKLSP 360

Query: 361 KCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQV-EEFLG 420
           KCVVLVEEELFKVSK QPMSFVEFFFEAFHHFS+LSDSLLRCF G +E+GF+QV +EFLG
Sbjct: 361 KCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVHENGFKQVMDEFLG 420

Query: 421 ARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQHE 480
            RILESV QFPC  N  NL    FD YLKGYKKIPFSSFN  QAKYL++LFRGDFWVQHE
Sbjct: 421 TRILESVSQFPCGKNAMNLREKAFD-YLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHE 480

Query: 481 KCRLSLCWKSRPLCTATIWVPRVK 489
              LSLCWKSRPLC+ATIWVP  K
Sbjct: 481 NGTLSLCWKSRPLCSATIWVPTAK 494

BLAST of MC01g0721 vs. ExPASy TrEMBL
Match: A0A5D3DPX1 (Nodulation-signaling pathway 2 protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00340 PE=3 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 9.27e-218
Identity = 347/517 (67.12%), Postives = 387/517 (74.85%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPA-ETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLE 60
           +N+ME E +EF TNY+YF + E F  THE      GNS C S SEN SSQNS Q+GSFLE
Sbjct: 1   MNTMEFESMEFFTNYNYFSSPEAFFHTHE------GNS-CSSLSEN-SSQNSFQEGSFLE 60

Query: 61  YGDQDLIFLEEALEFDPTNYNPDFLSTSTDQNPA-------------EMDSWIQPNEKDG 120
           Y DQ        L+FDP NYNP+ L   T  +P              E D   + +E + 
Sbjct: 61  YDDQSF------LQFDPINYNPEDLLAVTSIDPQNNQELSDHSGHVDEEDHEEEQDESNN 120

Query: 121 SFFEGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAW 180
             F+GIQAELMEEESLTDLLLAAAEA+EAQN  LVS LIEKLKNLLL D GSS FN+LAW
Sbjct: 121 IIFKGIQAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAW 180

Query: 181 FFTQGLHYKSTLTNFFPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQG 240
           FFTQGLHYK+   N             NS+SAFQMLQ+LSPYIKFAHFTANQAILEAA+G
Sbjct: 181 FFTQGLHYKTVDNNILAAHHQIQINNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEG 240

Query: 241 EEALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETKI-EQTGRRLSEFA 300
           E  +H+IDFDIMEGIQWPPLMADLAAK+    LRLTA+VQD+ENE KI EQTGRRLSEFA
Sbjct: 241 ERMIHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFA 300

Query: 301 KSINFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKL 360
           KS+N PF FD + IEKA+ F +IQV G+ VIANCSGI HHILS+ N+SK  TFL G +KL
Sbjct: 301 KSVNLPFIFDQIGIEKADKFEEIQVMGETVIANCSGIFHHILSYENLSKFGTFLDGVSKL 360

Query: 361 SPKCVVLVEEELFKVSK------PQP-MSFVEFFFEAFHHFSALSDSLLRCFCGAYESGF 420
           SPKCVVLVEEELF V+K      PQP MSFVEFFFEAFHHFSALSDSLLRCF G YE+GF
Sbjct: 361 SPKCVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSGVYENGF 420

Query: 421 RQV-EEFLGARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLF 480
           +QV +EFLG RIL+SV QFPCD        +GFDH LKGYKKIPFSSFN  QAKYL+SLF
Sbjct: 421 KQVMDEFLGTRILDSVTQFPCD-KTHVSWGSGFDH-LKGYKKIPFSSFNCSQAKYLISLF 480

Query: 481 RGDFWVQHEKCRLSLCWKSRPLCTATIWVPRVKSGAK 493
           RGDFWVQHEKC LSLCWKSRPLCTATIWVP V+S  K
Sbjct: 481 RGDFWVQHEKCSLSLCWKSRPLCTATIWVPCVESWTK 501

BLAST of MC01g0721 vs. ExPASy TrEMBL
Match: A0A1S3B384 (nodulation-signaling pathway 2 protein-like OS=Cucumis melo OX=3656 GN=LOC103485258 PE=3 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 9.27e-218
Identity = 347/517 (67.12%), Postives = 387/517 (74.85%), Query Frame = 0

Query: 1   LNSMELEPIEFLTNYSYFPA-ETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQDGSFLE 60
           +N+ME E +EF TNY+YF + E F  THE      GNS C S SEN SSQNS Q+GSFLE
Sbjct: 1   MNTMEFESMEFFTNYNYFSSPEAFFHTHE------GNS-CSSLSEN-SSQNSFQEGSFLE 60

Query: 61  YGDQDLIFLEEALEFDPTNYNPDFLSTSTDQNPA-------------EMDSWIQPNEKDG 120
           Y DQ        L+FDP NYNP+ L   T  +P              E D   + +E + 
Sbjct: 61  YDDQSF------LQFDPINYNPEDLLAVTSIDPQNNQELSDHSGHVDEEDHEEEQDESNN 120

Query: 121 SFFEGIQAELMEEESLTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAW 180
             F+GIQAELMEEESLTDLLLAAAEA+EAQN  LVS LIEKLKNLLL D GSS FN+LAW
Sbjct: 121 IIFKGIQAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAW 180

Query: 181 FFTQGLHYKSTLTNFFPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQG 240
           FFTQGLHYK+   N             NS+SAFQMLQ+LSPYIKFAHFTANQAILEAA+G
Sbjct: 181 FFTQGLHYKTVDNNILAAHHQIQINNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEG 240

Query: 241 EEALHVIDFDIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETKI-EQTGRRLSEFA 300
           E  +H+IDFDIMEGIQWPPLMADLAAK+    LRLTA+VQD+ENE KI EQTGRRLSEFA
Sbjct: 241 ERMIHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFA 300

Query: 301 KSINFPFEFDHMRIEKAENFGKIQV-GQAVIANCSGILHHILSHSNISKLETFLSGAAKL 360
           KS+N PF FD + IEKA+ F +IQV G+ VIANCSGI HHILS+ N+SK  TFL G +KL
Sbjct: 301 KSVNLPFIFDQIGIEKADKFEEIQVMGETVIANCSGIFHHILSYENLSKFGTFLDGVSKL 360

Query: 361 SPKCVVLVEEELFKVSK------PQP-MSFVEFFFEAFHHFSALSDSLLRCFCGAYESGF 420
           SPKCVVLVEEELF V+K      PQP MSFVEFFFEAFHHFSALSDSLLRCF G YE+GF
Sbjct: 361 SPKCVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSGVYENGF 420

Query: 421 RQV-EEFLGARILESVRQFPCDDNVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLF 480
           +QV +EFLG RIL+SV QFPCD        +GFDH LKGYKKIPFSSFN  QAKYL+SLF
Sbjct: 421 KQVMDEFLGTRILDSVTQFPCD-KTHVSWGSGFDH-LKGYKKIPFSSFNCSQAKYLISLF 480

Query: 481 RGDFWVQHEKCRLSLCWKSRPLCTATIWVPRVKSGAK 493
           RGDFWVQHEKC LSLCWKSRPLCTATIWVP V+S  K
Sbjct: 481 RGDFWVQHEKCSLSLCWKSRPLCTATIWVPCVESWTK 501

BLAST of MC01g0721 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 169.9 bits (429), Expect = 5.3e-42
Identity = 156/493 (31.64%), Postives = 245/493 (49.70%), Query Frame = 0

Query: 18  FPAETFIQTHEFAEHSHGNSSCYSHSENNSSQNSLQ---DGSFLE-----YGDQDLIFLE 77
           +P E F+    F+ H+   ++  S S N  S N L    D  F +      GD+  + +E
Sbjct: 3   YPYEDFLDLF-FSTHTDPLATAASTSSNGYSLNDLDIDWDCDFRDVIESIMGDEGAM-ME 62

Query: 78  EALEFDPTNYNPDFL--STSTDQNPAEMDSWIQPNEKDGSFFEGIQAELMEEESLTDLLL 137
              E  P  ++ + L  S ST  + A+  S+ +P   +    +G++        L  LL+
Sbjct: 63  PESEAVPMLHDQEGLCNSASTGLSVADGVSFGEPKTDES---KGLR--------LVHLLV 122

Query: 138 AAAEAVEAQN--QPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNFFPPR 197
           AAA+A    N  + L  V++ +LK+ L+     +   RLA  FT GL       +   P+
Sbjct: 123 AAADASTGANKSRELTRVILARLKD-LVSPGDRTNMERLAAHFTNGLSKLLERDSVLCPQ 182

Query: 198 EMADQ--EKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGIQW 257
           +  D   ++ + ISAF++LQ +SPY+ F + TA QAILEA + E  +H++D+DI EG+QW
Sbjct: 183 QHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDINEGVQW 242

Query: 258 PPLMADLAAKK---DFSLLRLTAV---VQDHENETKIEQTGRRLSEFAKSINFPFEFDHM 317
             LM  L ++        LR+TA+       ++   +++TGRRL+ FA SI  PF + H 
Sbjct: 243 ASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIGQPFSYQHC 302

Query: 318 RIEK---AENFGKIQVGQAVIANCSGILH-HILSHSNISKLETFLSGAAKLSPKCVVLVE 377
           +++    + +  K+  G+AV+ NC  +LH    SH   S + +FLS A  L+PK V LV 
Sbjct: 303 KLDTNAFSTSSLKLVRGEAVVINC--MLHLPRFSHQTPSSVISFLSEAKTLNPKLVTLVH 362

Query: 378 EELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESVR 437
           EE+  +       F+  F +  H FSA+ DSL      A  +       F+G  +   + 
Sbjct: 363 EEVGLMGN---QGFLYRFMDLLHQFSAIFDSLEAGLSIANPARGFVERVFIGPWVANWLT 422

Query: 438 QFPCDD-NVRNLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRGDFWVQH-EKCRLSL 485
           +   +D  V +  S        G+K +  S  NRCQAK L+SLF   F V+   +  L L
Sbjct: 423 RITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFRVEELGQNGLVL 476

BLAST of MC01g0721 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-34
Identity = 132/408 (32.35%), Postives = 197/408 (48.28%), Query Frame = 0

Query: 110 EGIQAELMEEES--LTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPF----NR 169
           E I+ +  +EE   L  LLL  AEAV A N      L E  K LL     S+P+     R
Sbjct: 276 EEIKRQKQDEEGLHLLTLLLQCAEAVSADN------LEEANKLLLEISQLSTPYGTSAQR 335

Query: 170 LAWFFTQGLHYK---STLTNF--FPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQ 229
           +A +F++ +  +   S L  +   P R M        +SAFQ+   +SP +KF+HFTANQ
Sbjct: 336 VAAYFSEAMSARLLNSCLGIYAALPSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQ 395

Query: 230 AILEAAQGEEALHVIDFDIMEGIQWPPLMADLAAKKDF-SLLRLTAVVQDHENETKIEQT 289
           AI EA + E+++H+ID DIM+G+QWP L   LA++      +RLT +    E    ++ T
Sbjct: 396 AIQEAFEKEDSVHIIDLDIMQGLQWPGLFHILASRPGGPPHVRLTGLGTSME---ALQAT 455

Query: 290 GRRLSEFAKSINFPFEFDHMRIEKAENFGKIQVGQAVIANCSGILHHILSHS--NISKLE 349
           G+RLS+FA  +  PFEF  +    AE  G +   +  +     +  H L HS  +++  +
Sbjct: 456 GKRLSDFADKLGLPFEFCPL----AEKVGNLDTERLNVRKREAVAVHWLQHSLYDVTGSD 515

Query: 350 TF-LSGAAKLSPKCVVLVEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYE 409
              L    +L+PK V +VE++L         SF+  F EA H++SAL DSL   +    E
Sbjct: 516 AHTLWLLQRLAPKVVTVVEQDLSHAG-----SFLGRFVEAIHYYSALFDSLGASY--GEE 575

Query: 410 SGFRQVEEFLGARILESVRQFPCDDNVRNLLSNG---------FDHYLK-----GYKKIP 469
           S  R V           V Q      +RN+L+ G         F+ + +     G+K I 
Sbjct: 576 SEERHV-----------VEQQLLSKEIRNVLAVGGPSRSGEVKFESWREKMQQCGFKGIS 635

Query: 470 FSSFNRCQAKYLVSLFRGD-FWVQHEKCRLSLCWKSRPLCTATIWVPR 488
            +     QA  L+ +F  D + +  +   L L WK   L TA+ W PR
Sbjct: 636 LAGNAATQATLLLGMFPSDGYTLVDDNGTLKLGWKDLSLLTASAWTPR 652

BLAST of MC01g0721 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-30
Identity = 114/384 (29.69%), Postives = 174/384 (45.31%), Query Frame = 0

Query: 122 LTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLH------YK 181
           L  LLL  AE V   +    S L+ ++   +    GSSP  R+  +F Q L       Y 
Sbjct: 40  LLSLLLQCAEYVATDHLREASTLLSEISE-ICSPFGSSP-ERVVAYFAQALQTRVISSYL 99

Query: 182 STLTNFFPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDF 241
           S   +    + +   + +   SA Q    +SP IKF+HFTANQAI +A  GE+++H+ID 
Sbjct: 100 SGACSPLSEKPLTVVQSQKIFSALQTYNSVSPLIKFSHFTANQAIFQALDGEDSVHIIDL 159

Query: 242 DIMEGIQWPPLMADLAAKKDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFD 301
           D+M+G+QWP L   LA++     LR   +     +   +  TGRRL++FA S+N PFEF 
Sbjct: 160 DVMQGLQWPALFHILASRP--RKLRSIRITGFGSSSDLLASTGRRLADFASSLNLPFEFH 219

Query: 302 HMRIEKAENFGKIQV----GQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVL 361
            +           Q+    G+AV+ +    + H L     + LET L    +L P  + +
Sbjct: 220 PIEGIIGNLIDPSQLATRQGEAVVVHW---MQHRLYDVTGNNLET-LEILRRLKPNLITV 279

Query: 362 VEEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILES 421
           VE+EL   S     SF+  F EA H++SAL D+L          G    E F       +
Sbjct: 280 VEQEL---SYDDGGSFLGRFVEALHYYSALFDAL------GDGLGEESGERF-------T 339

Query: 422 VRQFPCDDNVRNLLSNGFDHYLK----------GYKKIPFSSFNRCQAKYLVSLFR-GDF 481
           V Q      +RN++++G     +          G++ +        QA  L+ +     +
Sbjct: 340 VEQIVLGTEIRNIVAHGGGRRKRMKWKEELSRVGFRPVSLRGNPATQAGLLLGMLPWNGY 399

Query: 482 WVQHEKCRLSLCWKSRPLCTATIW 485
            +  E   L L WK   L TA+ W
Sbjct: 400 TLVEENGTLRLGWKDLSLLTASAW 399

BLAST of MC01g0721 vs. TAIR 10
Match: AT1G66350.1 (RGA-like 1 )

HSP 1 Score: 130.6 bits (327), Expect = 3.5e-30
Identity = 116/381 (30.45%), Postives = 180/381 (47.24%), Query Frame = 0

Query: 122 LTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNF 181
           L   LLA AEAV+  N  L   L++ +   LL  S +    ++A +F +GL  +  +   
Sbjct: 152 LVHALLACAEAVQQNNLKLADALVKHVG--LLASSQAGAMRKVATYFAEGLARR--IYRI 211

Query: 182 FPPREMADQEKKNSISAFQMLQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEGI 241
           +P  ++A     +++       E  PY+KFAHFTANQAILE     E +HVID  +  G+
Sbjct: 212 YPRDDVALSSFSDTLQIH--FYESCPYLKFAHFTANQAILEVFATAEKVHVIDLGLNHGL 271

Query: 242 QWPPLMADLAAK----KDFSLLRLTAVVQDHENETKIEQTGRRLSEFAKSINFPFEFDHM 301
           QWP L+  LA +     DF   RLT +     + T I++ G +L + A +I   FEF  +
Sbjct: 272 QWPALIQALALRPNGPPDF---RLTGI---GYSLTDIQEVGWKLGQLASTIGVNFEFKSI 331

Query: 302 RIEKAENFG----KIQVG-QAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLV 361
            +    +       I+ G ++V  N    LH +L+H     ++ FLS    + P  + +V
Sbjct: 332 ALNNLSDLKPEMLDIRPGLESVAVNSVFELHRLLAHP--GSIDKFLSTIKSIRPDIMTVV 391

Query: 362 EEELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEEFLGARILESV 421
           E+E    +      F++ F E+ H++S+L DSL     G         E FLG +IL  V
Sbjct: 392 EQE----ANHNGTVFLDRFTESLHYYSSLFDSLE----GPPSQDRVMSELFLGRQILNLV 451

Query: 422 RQFPCDDNVR-------NLLSNGFDHYLKGYKKIPFSSFNRCQAKYLVSLFRG--DFWVQ 481
               C+   R       N   N F   L G+K +   S    QA  L++L+ G   + V+
Sbjct: 452 ---ACEGEDRVERHETLNQWRNRFG--LGGFKPVSIGSNAYKQASMLLALYAGADGYNVE 505

Query: 482 HEKCRLSLCWKSRPLCTATIW 485
             +  L L W++RPL   + W
Sbjct: 512 ENEGCLLLGWQTRPLIATSAW 505

BLAST of MC01g0721 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 130.6 bits (327), Expect = 3.5e-30
Identity = 112/376 (29.79%), Postives = 183/376 (48.67%), Query Frame = 0

Query: 122 LTDLLLAAAEAVEAQNQPLVSVLIEKLKNLLLCDSGSSPFNRLAWFFTQGLHYKSTLTNF 181
           L   L+A AEA+  +N  L   L++++  L    +G+    ++A +F Q L  +    ++
Sbjct: 180 LVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGA--MGKVATYFAQAL-ARRIYRDY 239

Query: 182 FPPREMADQEKKNSISAFQM-LQELSPYIKFAHFTANQAILEAAQGEEALHVIDFDIMEG 241
               ++      +     +M   E  PY+KFAHFTANQAILEA      +HVID  + +G
Sbjct: 240 TAETDVCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQG 299

Query: 242 IQWPPLMADLAAKKDF-SLLRLTAV-VQDHENETKIEQTGRRLSEFAKSINFPFEFDHMR 301
           +QWP LM  LA +       RLT +     EN   ++Q G +L++FA+++   FEF  + 
Sbjct: 300 MQWPALMQALALRPGGPPSFRLTGIGPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLA 359

Query: 302 IE-----KAENFGKIQVGQAVIANCSGILHHILSHSNISKLETFLSGAAKLSPKCVVLVE 361
            E     + E F      + ++ N    LH +L+ S    +E  L+    + P  V +VE
Sbjct: 360 AESLSDLEPEMFETRPESETLVVNSVFELHRLLARS--GSIEKLLNTVKAIKPSIVTVVE 419

Query: 362 EELFKVSKPQPMSFVEFFFEAFHHFSALSDSLLRCFCGAYESGFRQVEE-FLGARILESV 421
           +E    +    + F++ F EA H++S+L DSL   +  +  S  R + E +LG +IL  V
Sbjct: 420 QE----ANHNGIVFLDRFNEALHYYSSLFDSLEDSY--SLPSQDRVMSEVYLGRQILNVV 479

Query: 422 RQFPCDDNVRNLLSNGFDHYLK--GYKKIPFSSFNRCQAKYLVSLF-RGD-FWVQHEKCR 481
                D   R+  +  +   +K  G+  I   S    QA  L+SL+  GD + V+     
Sbjct: 480 AAEGSDRVERHETAAQWRIRMKSAGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGC 539

Query: 482 LSLCWKSRPLCTATIW 485
           L + W++RPL T + W
Sbjct: 540 LMIGWQTRPLITTSAW 544