MC00g0851 (gene) Bitter gourd (Dali-11) v1

BLAST of MC00g0851 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.5e-27
Identity = 107/342 (31.29%), Postives = 161/342 (47.08%), Query Frame = 0

Query: 1   LALFSRR----VSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRG 60
           L+L SR+    V+ AQ       ++  V  FT  +LK  T NF   ++IG    G +++G
Sbjct: 25  LSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKG 84

Query: 61  QLRSSWSTAEEARN---VTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYC 120
            L  S  T  +      + VK  +Q+  F        E+  L Q S   HP+   L+GYC
Sbjct: 85  WLDESTLTPTKPGTGLVIAVKKLNQE-GFQGHREWLTEINYLGQLS---HPNLVKLIGYC 144

Query: 121 CEDEVKGVVYDLNPLGFLQNLMTRDN-----FNWLQRVDVILELARLLDFIHWQEKPSLA 180
            EDE + +VY+    G L+N + R         W  RV+V L+ A+ L F+H      + 
Sbjct: 145 LEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIY 204

Query: 181 FDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSS 240
            D +ASNILLD +   KL  F    +  + DL+          GY+ P           S
Sbjct: 205 RDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARS 264

Query: 241 DVYSLGEILLGLIA-KRDLEHEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQ 300
           DVYS G +LL +++ KR L+H +  K++      +LV  WA+      R    +V   L 
Sbjct: 265 DVYSFGVLLLEILSGKRALDHNRPAKEE------NLVD-WARPYLTSKRKVLLIVDNRL- 324

Query: 301 EDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQ 327
            D  Y  EE VR+  +A+ C+ F P +RP+  QV++ L+ LQ
Sbjct: 325 -DTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQ 353

BLAST of MC00g0851 vs. ExPASy Swiss-Prot
Match: Q9CAH1 (Putative receptor-like protein kinase At1g72540 OS=Arabidopsis thaliana OX=3702 GN=At1g72540 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 9.4e-27
Identity = 99/348 (28.45%), Postives = 162/348 (46.55%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVS-------------VFTSQDLKAFTDNFNGKNLIGI 60
           L L SR+ S+   +  +D +N  +S             +FT ++LK  T  F+  N +G 
Sbjct: 33  LLLLSRQTSVPSRVYMSDFSNSTISLNDFSNSFFINIHIFTYEELKTITQGFSKYNFLGE 92

Query: 61  THSGMLYRGQLRSSWSTAEEARNVTVKIWDQK-----PEFACECLVREEVKLLTQPSLKG 120
              G +Y+G +  S  T  + + V VK   ++      E+  E ++  ++K         
Sbjct: 93  GGFGEVYKGFVDDSLKTGLKDQPVAVKALKREGGQGHREWLAEVIILGQLK--------- 152

Query: 121 HPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTR---DNFNWLQRVDVILELARLLDFI 180
           HP   NLVGYCCED+ + +VY+    G L++ + +       WL RV ++L  A+ L+F+
Sbjct: 153 HPHLVNLVGYCCEDDERLLVYEYMERGNLEDHLFQKYGGALPWLTRVKILLGAAKGLEFL 212

Query: 181 HWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIR 240
           H QEKP +  DF+ SNILL  +   KL  F   ++ +  + +   K      GY+ P   
Sbjct: 213 HKQEKPVIYRDFKPSNILLSSDFSSKLSDFGLATDGSEEEDSNFTKSVMGTEGYAAPEYI 272

Query: 241 GGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYR-PN--A 300
                   SDV+S G +LL ++  R    EK   Q    +V      WA+   + PN   
Sbjct: 273 SAGNLTTMSDVFSFGVVLLEMLTARKAV-EKYRAQRGRNLVE-----WARPMLKDPNKLE 332

Query: 301 SLVHETLQEDWGYNAEEGVR-ITELAMHCIEFFPSNRPSTKQVLQHLE 324
            ++  +L+   G  + EG+R    LA  C+   P +RP+   V++ LE
Sbjct: 333 RIIDPSLE---GKYSVEGIRKAAALAYQCLSHNPKSRPTMTTVVKTLE 362

BLAST of MC00g0851 vs. ExPASy Swiss-Prot
Match: F4JZW1 (Serine/threonine-protein kinase PBL13 OS=Arabidopsis thaliana OX=3702 GN=PBL13 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-24
Identity = 88/324 (27.16%), Postives = 148/324 (45.68%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           S   A   + VFT  +L+  T +F+  N +G    G +++G +        +A+ V VK+
Sbjct: 53  SISLAGSDLHVFTQAELRVITQSFSSSNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKL 112

Query: 76  WD-----QKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGF 135
            D        EF  E +   ++K         HP+   L+GYCCE+  + +VY+  P G 
Sbjct: 113 LDLDGLQGHREFMTEVMCLGKLK---------HPNLVKLIGYCCEEAHRLLVYEFMPRGS 172

Query: 136 LQNLMTRD---NFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLC 195
           L++ + R       W  R+++  E A+ L F+H  EKP +  DF+ASNILLD +   KL 
Sbjct: 173 LESQLFRRCSLPLPWTTRLNIAYEAAKGLQFLHEAEKPIIYRDFKASNILLDSDYTAKLS 232

Query: 196 GFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLI-AKRDL 255
            F    +    D            GY+ P           SDVYS G +LL L+  ++ +
Sbjct: 233 DFGLAKDGPQGDDTHVSTRVMGTQGYAAPEYIMTGHLTAKSDVYSFGVVLLELLTGRKSV 292

Query: 256 EHEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQEDWGYNAEEGVR-ITELAM 315
           +  +  +++      +LV  WA+   N+ R    ++   L++ +   +E G R    LA 
Sbjct: 293 DIARSSRKE------TLVE-WARPMLNDARKLGRIMDPRLEDQY---SETGARKAATLAY 352

Query: 316 HCIEFFPSNRPSTKQVLQHLEGLQ 327
            C+ + P  RP    V+  L+ ++
Sbjct: 353 QCLRYRPKTRPDISTVVSVLQDIK 357

BLAST of MC00g0851 vs. ExPASy Swiss-Prot
Match: O64842 (Probable serine/threonine-protein kinase PBL12 OS=Arabidopsis thaliana OX=3702 GN=PBL12 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.4e-24
Identity = 92/312 (29.49%), Postives = 142/312 (45.51%), Query Frame = 0

Query: 21  NMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKIWD-QK 80
           + K+ +FT  +L+  T NF+  N++G    G +Y+G +        EA+ V VK  D   
Sbjct: 70  SQKLRLFTLSELRVITHNFSRSNMLGEGGFGPVYKGFIDDKVKPGIEAQPVAVKALDLHG 129

Query: 81  PEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDN 140
            +   E L   E+  L Q S K       L+G+CCE+E + +VY+  P G L+N + R N
Sbjct: 130 HQGHREWLA--EILFLGQLSNK---HLVKLIGFCCEEEQRVLVYEYMPRGSLENQLFRRN 189

Query: 141 ---FNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNET 200
                W  R+ + L  A+ L F+H  EKP +  DF+ SNILLD +   KL  F    +  
Sbjct: 190 SLAMAWGIRMKIALGAAKGLAFLHEAEKPVIYRDFKTSNILLDSDYNAKLSDFGLAKDGP 249

Query: 201 VSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLI-AKRDLEHEKLEKQD 260
             +            GY+ P           +DVYS G +LL LI  KR +++ +  ++ 
Sbjct: 250 EGEHTHVTTRVMGTQGYAAPEYIMTGHLTTMNDVYSFGVVLLELITGKRSMDNTRTRRE- 309

Query: 261 HHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRI-TELAMHCIEFFPSNRPS 320
                 SLV  WA+   R    L           +  E  ++   LA  C+   P  RP+
Sbjct: 310 -----QSLVE-WARPMLRDQRKLERIIDPRLANQHKTEAAQVAASLAYKCLSQHPKYRPT 369

Query: 321 TKQVLQHLEGLQ 327
             +V++ LE +Q
Sbjct: 370 MCEVVKVLESIQ 369

BLAST of MC00g0851 vs. ExPASy Swiss-Prot
Match: Q9ZUF4 (Serine/threonine-protein kinase RIPK OS=Arabidopsis thaliana OX=3702 GN=RIPK PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.7e-24
Identity = 91/323 (28.17%), Postives = 141/323 (43.65%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           S   A   + VFT  +LK  T +F+  N +G    G +++G +        +A+ V VK+
Sbjct: 64  SISLAGSDLHVFTLAELKVITQSFSSTNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKL 123

Query: 76  WDQK-----PEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGF 135
            D +      E+  E +   ++K         H +   L+GYCCE+E + +VY+  P G 
Sbjct: 124 LDLEGLQGHREWLTEVMFLGQLK---------HKNLVKLIGYCCEEEHRTLVYEFMPRGS 183

Query: 136 LQNLMTR---DNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLC 195
           L+N + R    +  W  R+ +    A  L F+H  E P +  DF+ASNILLD +   KL 
Sbjct: 184 LENQLFRRYSASLPWSTRMKIAHGAATGLQFLHEAENPVIYRDFKASNILLDSDYTAKLS 243

Query: 196 GFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLE 255
            F    +    D            GY+ P           SDVYS G +LL L+  R   
Sbjct: 244 DFGLAKDGPEGDDTHVSTRVMGTQGYAAPEYIMTGHLTARSDVYSFGVVLLELLTGRRSV 303

Query: 256 HEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQEDWGYNAEEGVR-ITELAMH 315
            +K   ++ + V       WA+   N+ R  + ++   L+   G  +E G R    LA  
Sbjct: 304 DKKRSSREQNLV------DWARPMLNDPRKLSRIMDPRLE---GQYSETGARKAATLAYQ 363

Query: 316 CIEFFPSNRPSTKQVLQHLEGLQ 327
           C+   P NRP    V+  L  L+
Sbjct: 364 CLSHRPKNRPCMSAVVSILNDLK 368

BLAST of MC00g0851 vs. NCBI nr
Match: XP_022151893.1 (putative receptor-like protein kinase At1g72540 isoform X2 [Momordica charantia])

HSP 1 Score: 696 bits (1797), Expect = 1.31e-252
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 60
           LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS
Sbjct: 26  LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 85

Query: 61  SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG 120
           SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG
Sbjct: 86  SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG 145

Query: 121 VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW 180
           VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW
Sbjct: 146 VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW 205

Query: 181 ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL 240
           ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL
Sbjct: 206 ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL 265

Query: 241 IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL 300
           IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL
Sbjct: 266 IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL 325

Query: 301 AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK
Sbjct: 326 AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 367

BLAST of MC00g0851 vs. NCBI nr
Match: XP_022151892.1 (putative receptor-like protein kinase At1g72540 isoform X1 [Momordica charantia])

HSP 1 Score: 680 bits (1755), Expect = 1.05e-245
Identity = 342/373 (91.69%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 60
           LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS
Sbjct: 26  LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 85

Query: 61  SWSTAEEARNVTVKIWDQKPEFACECLVR------------------------------- 120
           SWSTAEEARNVTVKIWDQKPEFACECLVR                               
Sbjct: 86  SWSTAEEARNVTVKIWDQKPEFACECLVRVSRQILSYTLHLQTQLAVFLSKLKICFTSSQ 145

Query: 121 EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI 180
           EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI
Sbjct: 146 EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI 205

Query: 181 LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI 240
           LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI
Sbjct: 206 LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI 265

Query: 241 PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK 300
           PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK
Sbjct: 266 PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK 325

Query: 301 NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ 342
           NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ
Sbjct: 326 NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ 385

BLAST of MC00g0851 vs. NCBI nr
Match: XP_022945250.1 (serine/threonine-protein kinase BIK1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 479 bits (1232), Expect = 4.90e-167
Identity = 244/331 (73.72%), Postives = 268/331 (80.97%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+  +LIGIT SG  YRGQLRS+WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL T PSL GHPS A L+GYCCEDEVKGVVYDL+PLG L
Sbjct: 73  WDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGSL 132

Query: 136 QNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAP 195
           QNL+ RD+ NWLQRV+VILELARLLDFIH QEK SL   FRASNILLDWECKPKLCG   
Sbjct: 133 QNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCG--- 192

Query: 196 NSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKL 255
               T +DLN    FAQ PS Y H S RGGS+GKMS+D+YSLGEILLGLIAKR  E EKL
Sbjct: 193 ----TGADLNN---FAQNPSSYFHLSTRGGSQGKMSTDIYSLGEILLGLIAKRAYEPEKL 252

Query: 256 EKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSN 315
           E+++HH VVNSLVS WAKNEYRPN SLVHETLQ+DWGY AEEGV +TELA+H IEFFP N
Sbjct: 253 ERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELAIHSIEFFPRN 312

Query: 316 RPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           RPS KQ+LQHLEGLQV QRL+D RPRKKERK
Sbjct: 313 RPSIKQILQHLEGLQVTQRLSDTRPRKKERK 333

BLAST of MC00g0851 vs. NCBI nr
Match: KAG6573595.1 (Serine/threonine-protein kinase PBL13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012721.1 Serine/threonine-protein kinase PBL13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474 bits (1221), Expect = 2.31e-165
Identity = 242/331 (73.11%), Postives = 266/331 (80.36%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+  +LIGIT SG  YRGQLRS+WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL   PSL GHPS A L+GYCCEDEVKGVVYDL+PLG L
Sbjct: 73  WDQKPEFDNATTNEFLVREEVKLSAHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGSL 132

Query: 136 QNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAP 195
           QNL+ RD+ NWLQRV+VILELARLLDFIH QEK SL   FRASNILLDWECKPKLCG   
Sbjct: 133 QNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCG--- 192

Query: 196 NSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKL 255
               T +DLN    FAQ PS Y H S RGGS+GKMS+D+YSLGEILLGLIAKR  E EKL
Sbjct: 193 ----TGADLNN---FAQNPSSYFHLSTRGGSQGKMSTDIYSLGEILLGLIAKRAYEPEKL 252

Query: 256 EKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSN 315
           E+++HH VVNSLVS WAKNEYRPN SLVHETLQ+DWGY AEEGV +TELA+ CIEFFP N
Sbjct: 253 ERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELAIQCIEFFPRN 312

Query: 316 RPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           RPS KQ+LQ LEGLQV QRL+D RPRKKERK
Sbjct: 313 RPSIKQILQRLEGLQVTQRLSDTRPRKKERK 333

BLAST of MC00g0851 vs. NCBI nr
Match: XP_022966807.1 (probable serine/threonine-protein kinase CST [Cucurbita maxima])

HSP 1 Score: 472 bits (1215), Expect = 2.43e-164
Identity = 239/331 (72.21%), Postives = 266/331 (80.36%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+G +LIGIT S   YRGQLR++WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL T  SL GH S A L+GYCCEDEVKGVVYD +PLG L
Sbjct: 73  WDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVVYDFDPLGSL 132

Query: 136 QNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAP 195
           QNL+ RD+ NWLQRV+VILELARLLDFIH QEK SL   FRASNILLDWECKPKLCG   
Sbjct: 133 QNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCGLEV 192

Query: 196 NSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKL 255
            SN   +DLN    FAQ PS Y H S RGGS+GKMS+D++SLGEILLGLIAKRD E EKL
Sbjct: 193 ISNGAGADLNN---FAQNPSSYFHLSTRGGSQGKMSTDIFSLGEILLGLIAKRDYEPEKL 252

Query: 256 EKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSN 315
           E+++HH+VVNSLVS WAKNEYRPN SLVHETLQ+DWGY  EEGV +TELA+H IEFFP N
Sbjct: 253 ERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELAIHSIEFFPRN 312

Query: 316 RPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           RPS KQ+LQ LEGLQV QRL+D RPRKKERK
Sbjct: 313 RPSIKQILQRLEGLQVTQRLSDTRPRKKERK 340

BLAST of MC00g0851 vs. ExPASy TrEMBL
Match: A0A6J1DDE8 (putative receptor-like protein kinase At1g72540 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019749 PE=4 SV=1)

HSP 1 Score: 696 bits (1797), Expect = 6.35e-253
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 60
           LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS
Sbjct: 26  LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 85

Query: 61  SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG 120
           SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG
Sbjct: 86  SWSTAEEARNVTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKG 145

Query: 121 VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW 180
           VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW
Sbjct: 146 VVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDW 205

Query: 181 ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL 240
           ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL
Sbjct: 206 ECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGL 265

Query: 241 IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL 300
           IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL
Sbjct: 266 IAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITEL 325

Query: 301 AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK
Sbjct: 326 AMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 367

BLAST of MC00g0851 vs. ExPASy TrEMBL
Match: A0A6J1DEP7 (putative receptor-like protein kinase At1g72540 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019749 PE=4 SV=1)

HSP 1 Score: 680 bits (1755), Expect = 5.09e-246
Identity = 342/373 (91.69%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 60
           LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS
Sbjct: 26  LALFSRRVSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRS 85

Query: 61  SWSTAEEARNVTVKIWDQKPEFACECLVR------------------------------- 120
           SWSTAEEARNVTVKIWDQKPEFACECLVR                               
Sbjct: 86  SWSTAEEARNVTVKIWDQKPEFACECLVRVSRQILSYTLHLQTQLAVFLSKLKICFTSSQ 145

Query: 121 EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI 180
           EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI
Sbjct: 146 EEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVI 205

Query: 181 LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI 240
           LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI
Sbjct: 206 LELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQI 265

Query: 241 PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK 300
           PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK
Sbjct: 266 PSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAK 325

Query: 301 NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ 342
           NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ
Sbjct: 326 NEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQ 385

BLAST of MC00g0851 vs. ExPASy TrEMBL
Match: A0A6J1G0E5 (serine/threonine-protein kinase BIK1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449550 PE=4 SV=1)

HSP 1 Score: 479 bits (1232), Expect = 2.37e-167
Identity = 244/331 (73.72%), Postives = 268/331 (80.97%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+  +LIGIT SG  YRGQLRS+WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL T PSL GHPS A L+GYCCEDEVKGVVYDL+PLG L
Sbjct: 73  WDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGSL 132

Query: 136 QNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAP 195
           QNL+ RD+ NWLQRV+VILELARLLDFIH QEK SL   FRASNILLDWECKPKLCG   
Sbjct: 133 QNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCG--- 192

Query: 196 NSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKL 255
               T +DLN    FAQ PS Y H S RGGS+GKMS+D+YSLGEILLGLIAKR  E EKL
Sbjct: 193 ----TGADLNN---FAQNPSSYFHLSTRGGSQGKMSTDIYSLGEILLGLIAKRAYEPEKL 252

Query: 256 EKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSN 315
           E+++HH VVNSLVS WAKNEYRPN SLVHETLQ+DWGY AEEGV +TELA+H IEFFP N
Sbjct: 253 ERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELAIHSIEFFPRN 312

Query: 316 RPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           RPS KQ+LQHLEGLQV QRL+D RPRKKERK
Sbjct: 313 RPSIKQILQHLEGLQVTQRLSDTRPRKKERK 333

BLAST of MC00g0851 vs. ExPASy TrEMBL
Match: A0A6J1HNY6 (probable serine/threonine-protein kinase CST OS=Cucurbita maxima OX=3661 GN=LOC111466402 PE=4 SV=1)

HSP 1 Score: 472 bits (1215), Expect = 1.17e-164
Identity = 239/331 (72.21%), Postives = 266/331 (80.36%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+G +LIGIT S   YRGQLR++WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL T  SL GH S A L+GYCCEDEVKGVVYD +PLG L
Sbjct: 73  WDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVVYDFDPLGSL 132

Query: 136 QNLMTRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAP 195
           QNL+ RD+ NWLQRV+VILELARLLDFIH QEK SL   FRASNILLDWECKPKLCG   
Sbjct: 133 QNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWECKPKLCGLEV 192

Query: 196 NSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKL 255
            SN   +DLN    FAQ PS Y H S RGGS+GKMS+D++SLGEILLGLIAKRD E EKL
Sbjct: 193 ISNGAGADLNN---FAQNPSSYFHLSTRGGSQGKMSTDIFSLGEILLGLIAKRDYEPEKL 252

Query: 256 EKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSN 315
           E+++HH+VVNSLVS WAKNEYRPN SLVHETLQ+DWGY  EEGV +TELA+H IEFFP N
Sbjct: 253 ERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELAIHSIEFFPRN 312

Query: 316 RPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           RPS KQ+LQ LEGLQV QRL+D RPRKKERK
Sbjct: 313 RPSIKQILQRLEGLQVTQRLSDTRPRKKERK 340

BLAST of MC00g0851 vs. ExPASy TrEMBL
Match: A0A6J1G0A1 (putative receptor-like protein kinase At1g72540 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449550 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 1.72e-161
Identity = 244/356 (68.54%), Postives = 268/356 (75.28%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           +ADSAN  V +FTS+DLK  T NF+  +LIGIT SG  YRGQLRS+WST  EARN+TVKI
Sbjct: 13  AADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGTEARNLTVKI 72

Query: 76  WDQKPEF----ACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFL 135
           WDQKPEF      E LVREEVKL T PSL GHPS A L+GYCCEDEVKGVVYDL+PLG L
Sbjct: 73  WDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLDPLGSL 132

Query: 136 QNLMTR-------------------------DNFNWLQRVDVILELARLLDFIHWQEKPS 195
           QNL+ R                         D+ NWLQRV+VILELARLLDFIH QEK S
Sbjct: 133 QNLVARGNLQVHEPKNHCLRSSVPVFFFLLADDLNWLQRVEVILELARLLDFIHSQEKQS 192

Query: 196 LAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKM 255
           L   FRASNILLDWECKPKLCG       T +DLN    FAQ PS Y H S RGGS+GKM
Sbjct: 193 LPIKFRASNILLDWECKPKLCG-------TGADLNN---FAQNPSSYFHLSTRGGSQGKM 252

Query: 256 SSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETLQED 315
           S+D+YSLGEILLGLIAKR  E EKLE+++HH VVNSLVS WAKNEYRPN SLVHETLQ+D
Sbjct: 253 STDIYSLGEILLGLIAKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKD 312

Query: 316 WGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERK 342
           WGY AEEGV +TELA+H IEFFP NRPS KQ+LQHLEGLQV QRL+D RPRKKERK
Sbjct: 313 WGYTAEEGVELTELAIHSIEFFPRNRPSIKQILQHLEGLQVTQRLSDTRPRKKERK 358

BLAST of MC00g0851 vs. TAIR 10
Match: AT2G39660.1 (botrytis-induced kinase1 )

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-28
Identity = 107/342 (31.29%), Postives = 161/342 (47.08%), Query Frame = 0

Query: 1   LALFSRR----VSMAQMLGSADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRG 60
           L+L SR+    V+ AQ       ++  V  FT  +LK  T NF   ++IG    G +++G
Sbjct: 25  LSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKG 84

Query: 61  QLRSSWSTAEEARN---VTVKIWDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYC 120
            L  S  T  +      + VK  +Q+  F        E+  L Q S   HP+   L+GYC
Sbjct: 85  WLDESTLTPTKPGTGLVIAVKKLNQE-GFQGHREWLTEINYLGQLS---HPNLVKLIGYC 144

Query: 121 CEDEVKGVVYDLNPLGFLQNLMTRDN-----FNWLQRVDVILELARLLDFIHWQEKPSLA 180
            EDE + +VY+    G L+N + R         W  RV+V L+ A+ L F+H      + 
Sbjct: 145 LEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIY 204

Query: 181 FDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSS 240
            D +ASNILLD +   KL  F    +  + DL+          GY+ P           S
Sbjct: 205 RDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARS 264

Query: 241 DVYSLGEILLGLIA-KRDLEHEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQ 300
           DVYS G +LL +++ KR L+H +  K++      +LV  WA+      R    +V   L 
Sbjct: 265 DVYSFGVLLLEILSGKRALDHNRPAKEE------NLVD-WARPYLTSKRKVLLIVDNRL- 324

Query: 301 EDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQ 327
            D  Y  EE VR+  +A+ C+ F P +RP+  QV++ L+ LQ
Sbjct: 325 -DTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQ 353

BLAST of MC00g0851 vs. TAIR 10
Match: AT1G72540.1 (Protein kinase superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.7e-28
Identity = 99/348 (28.45%), Postives = 162/348 (46.55%), Query Frame = 0

Query: 1   LALFSRRVSMAQMLGSADSANMKVS-------------VFTSQDLKAFTDNFNGKNLIGI 60
           L L SR+ S+   +  +D +N  +S             +FT ++LK  T  F+  N +G 
Sbjct: 33  LLLLSRQTSVPSRVYMSDFSNSTISLNDFSNSFFINIHIFTYEELKTITQGFSKYNFLGE 92

Query: 61  THSGMLYRGQLRSSWSTAEEARNVTVKIWDQK-----PEFACECLVREEVKLLTQPSLKG 120
              G +Y+G +  S  T  + + V VK   ++      E+  E ++  ++K         
Sbjct: 93  GGFGEVYKGFVDDSLKTGLKDQPVAVKALKREGGQGHREWLAEVIILGQLK--------- 152

Query: 121 HPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTR---DNFNWLQRVDVILELARLLDFI 180
           HP   NLVGYCCED+ + +VY+    G L++ + +       WL RV ++L  A+ L+F+
Sbjct: 153 HPHLVNLVGYCCEDDERLLVYEYMERGNLEDHLFQKYGGALPWLTRVKILLGAAKGLEFL 212

Query: 181 HWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPSIR 240
           H QEKP +  DF+ SNILL  +   KL  F   ++ +  + +   K      GY+ P   
Sbjct: 213 HKQEKPVIYRDFKPSNILLSSDFSSKLSDFGLATDGSEEEDSNFTKSVMGTEGYAAPEYI 272

Query: 241 GGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYR-PN--A 300
                   SDV+S G +LL ++  R    EK   Q    +V      WA+   + PN   
Sbjct: 273 SAGNLTTMSDVFSFGVVLLEMLTARKAV-EKYRAQRGRNLVE-----WARPMLKDPNKLE 332

Query: 301 SLVHETLQEDWGYNAEEGVR-ITELAMHCIEFFPSNRPSTKQVLQHLE 324
            ++  +L+   G  + EG+R    LA  C+   P +RP+   V++ LE
Sbjct: 333 RIIDPSLE---GKYSVEGIRKAAALAYQCLSHNPKSRPTMTTVVKTLE 362

BLAST of MC00g0851 vs. TAIR 10
Match: AT5G35580.1 (Protein kinase superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 8.2e-26
Identity = 88/324 (27.16%), Postives = 148/324 (45.68%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           S   A   + VFT  +L+  T +F+  N +G    G +++G +        +A+ V VK+
Sbjct: 53  SISLAGSDLHVFTQAELRVITQSFSSSNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKL 112

Query: 76  WD-----QKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGF 135
            D        EF  E +   ++K         HP+   L+GYCCE+  + +VY+  P G 
Sbjct: 113 LDLDGLQGHREFMTEVMCLGKLK---------HPNLVKLIGYCCEEAHRLLVYEFMPRGS 172

Query: 136 LQNLMTRD---NFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLC 195
           L++ + R       W  R+++  E A+ L F+H  EKP +  DF+ASNILLD +   KL 
Sbjct: 173 LESQLFRRCSLPLPWTTRLNIAYEAAKGLQFLHEAEKPIIYRDFKASNILLDSDYTAKLS 232

Query: 196 GFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLI-AKRDL 255
            F    +    D            GY+ P           SDVYS G +LL L+  ++ +
Sbjct: 233 DFGLAKDGPQGDDTHVSTRVMGTQGYAAPEYIMTGHLTAKSDVYSFGVVLLELLTGRKSV 292

Query: 256 EHEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQEDWGYNAEEGVR-ITELAM 315
           +  +  +++      +LV  WA+   N+ R    ++   L++ +   +E G R    LA 
Sbjct: 293 DIARSSRKE------TLVE-WARPMLNDARKLGRIMDPRLEDQY---SETGARKAATLAY 352

Query: 316 HCIEFFPSNRPSTKQVLQHLEGLQ 327
            C+ + P  RP    V+  L+ ++
Sbjct: 353 QCLRYRPKTRPDISTVVSVLQDIK 357

BLAST of MC00g0851 vs. TAIR 10
Match: AT2G26290.1 (root-specific kinase 1 )

HSP 1 Score: 113.6 bits (283), Expect = 3.1e-25
Identity = 92/312 (29.49%), Postives = 142/312 (45.51%), Query Frame = 0

Query: 21  NMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKIWD-QK 80
           + K+ +FT  +L+  T NF+  N++G    G +Y+G +        EA+ V VK  D   
Sbjct: 70  SQKLRLFTLSELRVITHNFSRSNMLGEGGFGPVYKGFIDDKVKPGIEAQPVAVKALDLHG 129

Query: 81  PEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDN 140
            +   E L   E+  L Q S K       L+G+CCE+E + +VY+  P G L+N + R N
Sbjct: 130 HQGHREWLA--EILFLGQLSNK---HLVKLIGFCCEEEQRVLVYEYMPRGSLENQLFRRN 189

Query: 141 ---FNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNET 200
                W  R+ + L  A+ L F+H  EKP +  DF+ SNILLD +   KL  F    +  
Sbjct: 190 SLAMAWGIRMKIALGAAKGLAFLHEAEKPVIYRDFKTSNILLDSDYNAKLSDFGLAKDGP 249

Query: 201 VSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLI-AKRDLEHEKLEKQD 260
             +            GY+ P           +DVYS G +LL LI  KR +++ +  ++ 
Sbjct: 250 EGEHTHVTTRVMGTQGYAAPEYIMTGHLTTMNDVYSFGVVLLELITGKRSMDNTRTRRE- 309

Query: 261 HHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRI-TELAMHCIEFFPSNRPS 320
                 SLV  WA+   R    L           +  E  ++   LA  C+   P  RP+
Sbjct: 310 -----QSLVE-WARPMLRDQRKLERIIDPRLANQHKTEAAQVAASLAYKCLSQHPKYRPT 369

Query: 321 TKQVLQHLEGLQ 327
             +V++ LE +Q
Sbjct: 370 MCEVVKVLESIQ 369

BLAST of MC00g0851 vs. TAIR 10
Match: AT2G05940.1 (Protein kinase superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.1e-25
Identity = 91/323 (28.17%), Postives = 141/323 (43.65%), Query Frame = 0

Query: 16  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 75
           S   A   + VFT  +LK  T +F+  N +G    G +++G +        +A+ V VK+
Sbjct: 64  SISLAGSDLHVFTLAELKVITQSFSSTNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKL 123

Query: 76  WDQK-----PEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGF 135
            D +      E+  E +   ++K         H +   L+GYCCE+E + +VY+  P G 
Sbjct: 124 LDLEGLQGHREWLTEVMFLGQLK---------HKNLVKLIGYCCEEEHRTLVYEFMPRGS 183

Query: 136 LQNLMTR---DNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLC 195
           L+N + R    +  W  R+ +    A  L F+H  E P +  DF+ASNILLD +   KL 
Sbjct: 184 LENQLFRRYSASLPWSTRMKIAHGAATGLQFLHEAENPVIYRDFKASNILLDSDYTAKLS 243

Query: 196 GFAPNSNETVSDLNKPYKFAQIPSGYSHPSIRGGSRGKMSSDVYSLGEILLGLIAKRDLE 255
            F    +    D            GY+ P           SDVYS G +LL L+  R   
Sbjct: 244 DFGLAKDGPEGDDTHVSTRVMGTQGYAAPEYIMTGHLTARSDVYSFGVVLLELLTGRRSV 303

Query: 256 HEKLEKQDHHQVVNSLVSIWAK---NEYRPNASLVHETLQEDWGYNAEEGVR-ITELAMH 315
            +K   ++ + V       WA+   N+ R  + ++   L+   G  +E G R    LA  
Sbjct: 304 DKKRSSREQNLV------DWARPMLNDPRKLSRIMDPRLE---GQYSETGARKAATLAYQ 363

Query: 316 CIEFFPSNRPSTKQVLQHLEGLQ 327
           C+   P NRP    V+  L  L+
Sbjct: 364 CLSHRPKNRPCMSAVVSILNDLK 368