MC00g0476 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC00g0476
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 14-like
Locationscaffold196: 209406 .. 210623 (+)
RNA-Seq ExpressionMC00g0476
SyntenyMC00g0476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCTGCCGGACCCGATTCTGTTCGGCAAATACGAGCTCGGAAAGCTTCTGGGCTGCGGAGCCTTCGCCAAGGTCTACCACGCGAGAAACATCGCTACTGGAGAGAGCGTCGCGATCAAGGCCGTGAACAAGCTCAAGGTCGTCAATGGCGGATGCTCGGCGCACGTTAAGCGCGAGATCGCTATCATGCGCCGCCTCCGCCATCCTCACGTCGTCCGCCTCTACGAGGTTCTCGCCACCAAGACCAAGATTTACTTCGTGATGGAGTTCGCCAGAGGAGGGGAGCTCTTCGCGAAGGTTTCAAAGGGGCGATTCAGCGAGAATCTCAGCCGTCGCTACTTTCAGCAGCTGATCACCGCCGTCGGTTACTGCCATTCCAGAGGAATCTTCCACCGCGATCTGAAGCCGGAGAATCTCCTCCTCGACGACAATTTCGATCTCAGGGTAACGGATTTTGGACTCAGCGCCTTGACGGACCAGATTCGCCCCGACGGATTGCTCCACACGCTCTGCGGAACGCCGGCCTACGTCGCGCCGGAGATCCTCGCCAAGAAAGGCTACGACGGCGCCAAGGTCGACGTCTGGTCCTGCGGAATCATCCTCTACGTCCTCACCGCCGGCTACCTCCCCTTCAACGACTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCGAAGTGGACGTCTCCCGATCTCCGCCGATTTCTCTCCAGGCTTTTGGATACGAATCCCGCGACGAGGATAACCGTCGATGAGATTCTCCGCGATCCGTGGTTCAGTAAAGGATTTCAGGATCAGGAGAAGCTTCACCTCGAAGATTCCGCCTCTGATCTGTACGGCGATGTGAGTAACTCCAAATGCTTGAACGCTTTCGACCTCATCTCCTTCTCCCCTGGCTTCGACTTGACCGGATTGCTGAACGAGCCGGAGATTTCGGCCGGCGTCGAGAGATTCGTCTCCGCAGAGCCGCCGGAGAAGATTATTGGTAAAGTGGAGGAGGTTTCGGAGTCGGAGAGTGCGGCGGCGGTGGAGAAGAACGGATGGGGAGCTAAAGTGGAGAGCCTCGACGGCGCTTTCGTTGTAATTATAAATATTTTCCGATTGACGGAGGAATTGGTGGTCGTCGAGATTAAGAAGAAAGAGGTGGATCAAGAATTTCAAATTTGGAAGCACAAGCTCAGGCCTCAACTTGATTGCTTA

mRNA sequence

TCCCTGCCGGACCCGATTCTGTTCGGCAAATACGAGCTCGGAAAGCTTCTGGGCTGCGGAGCCTTCGCCAAGGTCTACCACGCGAGAAACATCGCTACTGGAGAGAGCGTCGCGATCAAGGCCGTGAACAAGCTCAAGGTCGTCAATGGCGGATGCTCGGCGCACGTTAAGCGCGAGATCGCTATCATGCGCCGCCTCCGCCATCCTCACGTCGTCCGCCTCTACGAGGTTCTCGCCACCAAGACCAAGATTTACTTCGTGATGGAGTTCGCCAGAGGAGGGGAGCTCTTCGCGAAGGTTTCAAAGGGGCGATTCAGCGAGAATCTCAGCCGTCGCTACTTTCAGCAGCTGATCACCGCCGTCGGTTACTGCCATTCCAGAGGAATCTTCCACCGCGATCTGAAGCCGGAGAATCTCCTCCTCGACGACAATTTCGATCTCAGGGTAACGGATTTTGGACTCAGCGCCTTGACGGACCAGATTCGCCCCGACGGATTGCTCCACACGCTCTGCGGAACGCCGGCCTACGTCGCGCCGGAGATCCTCGCCAAGAAAGGCTACGACGGCGCCAAGGTCGACGTCTGGTCCTGCGGAATCATCCTCTACGTCCTCACCGCCGGCTACCTCCCCTTCAACGACTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCGAAGTGGACGTCTCCCGATCTCCGCCGATTTCTCTCCAGGCTTTTGGATACGAATCCCGCGACGAGGATAACCGTCGATGAGATTCTCCGCGATCCGTGGTTCAGTAAAGGATTTCAGGATCAGGAGAAGCTTCACCTCGAAGATTCCGCCTCTGATCTGTACGGCGATGTGAGTAACTCCAAATGCTTGAACGCTTTCGACCTCATCTCCTTCTCCCCTGGCTTCGACTTGACCGGATTGCTGAACGAGCCGGAGATTTCGGCCGGCGTCGAGAGATTCGTCTCCGCAGAGCCGCCGGAGAAGATTATTGGTAAAGTGGAGGAGGTTTCGGAGTCGGAGAGTGCGGCGGCGGTGGAGAAGAACGGATGGGGAGCTAAAGTGGAGAGCCTCGACGGCGCTTTCGTTGTAATTATAAATATTTTCCGATTGACGGAGGAATTGGTGGTCGTCGAGATTAAGAAGAAAGAGGTGGATCAAGAATTTCAAATTTGGAAGCACAAGCTCAGGCCTCAACTTGATTGCTTA

Coding sequence (CDS)

TCCCTGCCGGACCCGATTCTGTTCGGCAAATACGAGCTCGGAAAGCTTCTGGGCTGCGGAGCCTTCGCCAAGGTCTACCACGCGAGAAACATCGCTACTGGAGAGAGCGTCGCGATCAAGGCCGTGAACAAGCTCAAGGTCGTCAATGGCGGATGCTCGGCGCACGTTAAGCGCGAGATCGCTATCATGCGCCGCCTCCGCCATCCTCACGTCGTCCGCCTCTACGAGGTTCTCGCCACCAAGACCAAGATTTACTTCGTGATGGAGTTCGCCAGAGGAGGGGAGCTCTTCGCGAAGGTTTCAAAGGGGCGATTCAGCGAGAATCTCAGCCGTCGCTACTTTCAGCAGCTGATCACCGCCGTCGGTTACTGCCATTCCAGAGGAATCTTCCACCGCGATCTGAAGCCGGAGAATCTCCTCCTCGACGACAATTTCGATCTCAGGGTAACGGATTTTGGACTCAGCGCCTTGACGGACCAGATTCGCCCCGACGGATTGCTCCACACGCTCTGCGGAACGCCGGCCTACGTCGCGCCGGAGATCCTCGCCAAGAAAGGCTACGACGGCGCCAAGGTCGACGTCTGGTCCTGCGGAATCATCCTCTACGTCCTCACCGCCGGCTACCTCCCCTTCAACGACTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCGAAGTGGACGTCTCCCGATCTCCGCCGATTTCTCTCCAGGCTTTTGGATACGAATCCCGCGACGAGGATAACCGTCGATGAGATTCTCCGCGATCCGTGGTTCAGTAAAGGATTTCAGGATCAGGAGAAGCTTCACCTCGAAGATTCCGCCTCTGATCTGTACGGCGATGTGAGTAACTCCAAATGCTTGAACGCTTTCGACCTCATCTCCTTCTCCCCTGGCTTCGACTTGACCGGATTGCTGAACGAGCCGGAGATTTCGGCCGGCGTCGAGAGATTCGTCTCCGCAGAGCCGCCGGAGAAGATTATTGGTAAAGTGGAGGAGGTTTCGGAGTCGGAGAGTGCGGCGGCGGTGGAGAAGAACGGATGGGGAGCTAAAGTGGAGAGCCTCGACGGCGCTTTCGTTGTAATTATAAATATTTTCCGATTGACGGAGGAATTGGTGGTCGTCGAGATTAAGAAGAAAGAGGTGGATCAAGAATTTCAAATTTGGAAGCACAAGCTCAGGCCTCAACTTGATTGCTTA

Protein sequence

SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREIAIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLISFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVESLDGAFVVIINIFRLTEELVVVEIKKKEVDQEFQIWKHKLRPQLDCL
Homology
BLAST of MC00g0476 vs. ExPASy Swiss-Prot
Match: Q9LZW4 (CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CIPK14 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.6e-153
Identity = 261/402 (64.93%), Postives = 326/402 (81.09%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREIAIMRRL 66
           LFGKYE+GKL+GCGAFAKVYH R+ ATG+SVAIK V+K ++  GG + +++REIAIM RL
Sbjct: 18  LFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMHRL 77

Query: 67  RHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHS 126
           RHP +VRL+EVLATK+KI+FVMEFA+GGELFAKVSKGRF E+LSRRYFQQLI+AVGYCHS
Sbjct: 78  RHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYCHS 137

Query: 127 RGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKG 186
           RGIFHRDLKPENLLLD+  DL+++DFGLSALTDQIRPDGLLHTLCGTPAYVAPE+LAKKG
Sbjct: 138 RGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAKKG 197

Query: 187 YDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRLL 246
           YDGAK+D+WSCGIIL+VL AGYLPFND NLM MYRKIY+G+FR PKWTSPDLRR L+RLL
Sbjct: 198 YDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTRLL 257

Query: 247 DTNPATRITVDEILRDPWFSKGFQDQ-EKLHLEDSASDLYGDVSNSKC----LNAFDLIS 306
           DTNP TRIT++EI+ DPWF +G+ D+  K HLEDS   L  D ++S+     +NAFD+IS
Sbjct: 258 DTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDIIS 317

Query: 307 FSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVESL 366
            SPGF+L+GL  +      VERFVSA   E+++ ++EE+  +E+    +K  WG K+E  
Sbjct: 318 GSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIEGQ 377

Query: 367 DGAFVVIINIFRLTEELVVVEIKKKE--VDQEFQIWKHKLRP 402
            G F +++ I +LT+ELV++E++K++        +W   LRP
Sbjct: 378 KGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRP 419

BLAST of MC00g0476 vs. ExPASy Swiss-Prot
Match: O22932 (CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana OX=3702 GN=CIPK11 PE=1 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.8e-134
Identity = 247/406 (60.84%), Postives = 317/406 (78.08%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVV-NGGCSAHVKREIAIMRR 66
           LFGKYELGKLLGCGAFAKV+HAR+  TG+SVA+K +NK K++ N   + ++KREI+IMRR
Sbjct: 17  LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRR 76

Query: 67  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSK-GRFSENLSRRYFQQLITAVGYC 126
           L HP++V+L+EV+ATK+KI+F MEF +GGELF K+SK GR SE+LSRRYFQQLI+AVGYC
Sbjct: 77  LSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYC 136

Query: 127 HSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 186
           H+RG++HRDLKPENLL+D+N +L+V+DFGLSALTDQIRPDGLLHTLCGTPAYVAPEIL+K
Sbjct: 137 HARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSK 196

Query: 187 KGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSR 246
           KGY+GAKVDVWSCGI+L+VL AGYLPFND N+M MY+KIY+G++R P+W SPDL+RF+SR
Sbjct: 197 KGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSR 256

Query: 247 LLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASD--LYGDVSNSKCLNAFDLISF 306
           LLD NP TRIT+DEIL+DPWF +G   Q K H +D   D  +   +   K LNAFDLIS+
Sbjct: 257 LLDINPETRITIDEILKDPWFVRGGFKQIKFH-DDEIEDQKVESSLEAVKSLNAFDLISY 316

Query: 307 SPGFDLTGLLNEPEISAG-VERFVSAEPPEKIIGKVEEVSESESAAAVEK--NGWGAKVE 366
           S G DL+GL      S+G  ERF+S + PE +  +VE  +  E+    +K    +G ++E
Sbjct: 317 SSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEME 376

Query: 367 SLDGAFVVIINIFRLTEELVVVEIKKKEVDQEF--QIWKHKLRPQL 404
             +G F + I I RL + LVVVE +++  D +   ++W  KLR QL
Sbjct: 377 GQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQL 421

BLAST of MC00g0476 vs. ExPASy Swiss-Prot
Match: Q9SN43 (CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=3702 GN=CIPK12 PE=1 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.6e-126
Identity = 234/443 (52.82%), Postives = 308/443 (69.53%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           S P  ++ G+YE+GKLLG G FAKVY ARN+ T ESVAIK ++K KV+ GG  AH+KREI
Sbjct: 16  SSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREI 75

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           +I+RR+RHP++V+L+EV+ATK KIYFVME+ RGGELF KV+KGR  E ++R+YFQQLI+A
Sbjct: 76  SILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISA 135

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           V +CH+RG++HRDLKPENLLLD+N +L+V+DFGLSA++DQIR DGL HT CGTPAYVAPE
Sbjct: 136 VTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPE 195

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           +LA+KGYD AKVD+WSCG+IL+VL AGYLPF+D N+MAMY+KIYRG+FRCP+W S +L R
Sbjct: 196 VLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTR 255

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQ---------------DQEKLHLEDSASDLY 300
            LS+LL+TNP  R T  EI+ + WF KGF+               D ++L  +   SD  
Sbjct: 256 LLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRD 315

Query: 301 GDVSNSK-----------------CLNAFDLISFSPGFDLTGLLNEPEISAGVERFVSAE 360
              S S+                  LNAFD+ISFS GFDL+GL ++        RFVS  
Sbjct: 316 SAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDD---DGEGSRFVSGA 375

Query: 361 PPEKIIGKVEEVSESESAAAVEKN---GWGAKVESLDGAFVVIINIFRLTEELVVVEIKK 407
           P  KII K+EE+++  S    +K+         + + G   +   IF LT  LVVVE+KK
Sbjct: 376 PVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKK 435

BLAST of MC00g0476 vs. ExPASy Swiss-Prot
Match: Q68Y49 (CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 8.9e-125
Identity = 232/449 (51.67%), Postives = 296/449 (65.92%), Query Frame = 0

Query: 6   ILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREIAIMRR 65
           +L G+YELG+LLG G FAKVY AR+  +GE VAIK ++K K +  G   H+KREIAI+RR
Sbjct: 32  LLLGRYELGRLLGHGTFAKVYQARSADSGEPVAIKVLDKEKAMRHGLVPHIKREIAILRR 91

Query: 66  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 125
           +RHP++VRL+EV+ATK+KIYFVME  RGGELF +V+KGR  E+ +RRYFQQL++AVG+CH
Sbjct: 92  VRHPNIVRLFEVMATKSKIYFVMELVRGGELFGRVAKGRLKEDTARRYFQQLVSAVGFCH 151

Query: 126 SRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 185
           +RG+FHRDLKPENLL+D++ DL+V+DFGLSA+ DQ  PDGLLHT CGTP+YVAPE+LA++
Sbjct: 152 ARGVFHRDLKPENLLVDEHGDLKVSDFGLSAVADQFHPDGLLHTFCGTPSYVAPEVLARR 211

Query: 186 GYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRL 245
           GYDGAK D+WSCGIIL+VL AGYLPF+D NLMAMYRKIYRG+FRCP+W S DL   L+R+
Sbjct: 212 GYDGAKADIWSCGIILFVLMAGYLPFHDQNLMAMYRKIYRGEFRCPRWFSKDLSSLLNRI 271

Query: 246 LDTNPATRITVDEILRDPWFSKGF-------QDQEKLHLEDSASDL-------------- 305
           LDTNP TRITV E++   WF KGF       +D +   L D  +D+              
Sbjct: 272 LDTNPETRITVKEVMESRWFQKGFRPVRFYVEDDQVHSLADGDNDMPELEPSEPPPPPPF 331

Query: 306 --------------------------------------YGDVSNSKCLNAFDLISFSPGF 365
                                                  G ++    LNAFD+ISFS GF
Sbjct: 332 PPPPPQQDDDGEESGWESDSSVASCPATLSSEERRQRPLGSLTRPASLNAFDIISFSKGF 391

Query: 366 DLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKV----ESLD 392
           DL+GL  E        RF+SAEP + II K+EE+++ +S   V +  W   +    E L 
Sbjct: 392 DLSGLFEE---RGSEVRFISAEPMQTIITKLEEIAKVKS-FFVRRKDWRVSIEGTREGLK 451

BLAST of MC00g0476 vs. ExPASy Swiss-Prot
Match: O80902 (CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CIPK22 PE=1 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.2e-124
Identity = 227/398 (57.04%), Postives = 293/398 (73.62%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIAT-GESVAIKAVNKLKVVNGGCSAHVKREIAIMRR 66
           LFGKY+LGKLLG GAFAKVY A ++   GESVAIK V K K +  G +AHVKREI++MRR
Sbjct: 24  LFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK-KRLKDGLTAHVKREISVMRR 83

Query: 67  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 126
           LRHPH+V L EVLATKTKIYFVME A+GGELF++V+  RF+E+LSR+YF+QLI+AV YCH
Sbjct: 84  LRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNRFTESLSRKYFRQLISAVRYCH 143

Query: 127 SRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 186
           +RG+FHRDLKPENLLLD+N DL+V+DFGLSA+ +QI PDG+LHTLCGTPAYVAPE+L KK
Sbjct: 144 ARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPDGMLHTLCGTPAYVAPELLLKK 203

Query: 187 GYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRL 246
           GYDG+K D+WSCG++L++L AGYLPF D N+M +YRKI++  ++ P WTS DLR+ L RL
Sbjct: 204 GYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIHKAQYKLPDWTSSDLRKLLRRL 263

Query: 247 LDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLISFSPG 306
           L+ NP  RITV+EIL+DPWF+ G    E + ++   +D Y    N K LNAFDLIS +  
Sbjct: 264 LEPNPELRITVEEILKDPWFNHGVDPSEIIGIQ---ADDYDLEENGKILNAFDLISSASS 323

Query: 307 FDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVESLDGAF 366
            +L+GL          ++FVS E    I+ KVEEV++  +    +K     K+E   G  
Sbjct: 324 SNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVAKQLNLRIAKKKERAIKLEGPHGVA 383

Query: 367 VVIINIFRLTEELVVVEIKKKEVDQEFQIWKHKLRPQL 404
            V++ + RLT ELV+VE+K K+ D    +W   LR +L
Sbjct: 384 NVVVKVRRLTNELVMVEMKNKQRDVGL-VWADALRQKL 416

BLAST of MC00g0476 vs. NCBI nr
Match: KAG7033738.1 (CBL-interacting serine/threonine-protein kinase 14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 707 bits (1824), Expect = 1.39e-254
Identity = 350/408 (85.78%), Postives = 378/408 (92.65%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 24  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 83

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 84  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 143

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 144 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 203

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 204 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 263

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KGFQ +EKL +EDSAS++YGDV+N+KCLNAFDLI
Sbjct: 264 FLSRLLDTNPETRITVDEIHQDPWFVKGFQ-EEKLQIEDSASEIYGDVNNAKCLNAFDLI 323

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 324 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 383

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+ +I+IFRLTEELVVVEIK +  EVDQ+ QIWK  LRPQL+CL
Sbjct: 384 LDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQLECL 429

BLAST of MC00g0476 vs. NCBI nr
Match: XP_022949666.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata])

HSP 1 Score: 706 bits (1821), Expect = 3.07e-254
Identity = 349/408 (85.54%), Postives = 378/408 (92.65%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KGFQ +EKL ++DSAS++YGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPETRITVDEIHQDPWFVKGFQ-EEKLQIDDSASEIYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+ +I+IFRLTEELVVVEIK +  EVDQ+ QIWK  LRPQL+CL
Sbjct: 377 LDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQLECL 422

BLAST of MC00g0476 vs. NCBI nr
Match: XP_023545198.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703 bits (1814), Expect = 3.57e-253
Identity = 348/408 (85.29%), Postives = 376/408 (92.16%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KGFQ +EKL +EDS S++YGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPETRITVDEIHQDPWFVKGFQ-EEKLQIEDSGSEIYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+ +I+IFRLTEELVVVEIK +  EVD + QIWK  LRPQL+CL
Sbjct: 377 LDGGFIAVIDIFRLTEELVVVEIKNRVREVDPDGQIWKDILRPQLECL 422

BLAST of MC00g0476 vs. NCBI nr
Match: XP_022978189.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita maxima])

HSP 1 Score: 703 bits (1814), Expect = 3.57e-253
Identity = 348/408 (85.29%), Postives = 377/408 (92.40%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KG Q +EKL +EDS S++YGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPETRITVDEIHQDPWFVKGLQ-EEKLQIEDSGSEIYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+V+I+IF+LTEELVVVEIK +  EVDQ+ QIWK  LRPQL+CL
Sbjct: 377 LDGGFIVVIDIFQLTEELVVVEIKNRVREVDQDGQIWKDILRPQLECL 422

BLAST of MC00g0476 vs. NCBI nr
Match: XP_038883854.1 (CBL-interacting serine/threonine-protein kinase 14 [Benincasa hispida])

HSP 1 Score: 695 bits (1794), Expect = 4.12e-250
Identity = 341/408 (83.58%), Postives = 374/408 (91.67%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG++VAIKAVNKLKV+ GG +AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQNVAIKAVNKLKVLKGGFTAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELFAKVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSKGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDF CPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFHCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI RDPWF KGFQ+++   +ED +S+LYGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPDTRITVDEIHRDPWFVKGFQEEKFQQIEDFSSELYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VE V++ E+ A VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVENVAKLENVA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
            DG FV +I++FRLT+ELVVVEIK +  E+DQ+ Q WK  LRPQLDCL
Sbjct: 377 RDGGFVTVIDVFRLTDELVVVEIKNRVREIDQDRQTWKDVLRPQLDCL 423

BLAST of MC00g0476 vs. ExPASy TrEMBL
Match: A0A6J1GCM3 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452989 PE=3 SV=1)

HSP 1 Score: 706 bits (1821), Expect = 1.48e-254
Identity = 349/408 (85.54%), Postives = 378/408 (92.65%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KGFQ +EKL ++DSAS++YGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPETRITVDEIHQDPWFVKGFQ-EEKLQIDDSASEIYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+ +I+IFRLTEELVVVEIK +  EVDQ+ QIWK  LRPQL+CL
Sbjct: 377 LDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQLECL 422

BLAST of MC00g0476 vs. ExPASy TrEMBL
Match: A0A6J1ITF4 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111478246 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 1.73e-253
Identity = 348/408 (85.29%), Postives = 377/408 (92.40%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIK VNKLKV+ GG  AHVKREI
Sbjct: 17  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNKLKVLKGGFKAHVKREI 76

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVMEFA+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 77  AIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGRFSENLSRRYFQQLITA 136

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHS+GIFHRDLKPENLLLDDNFDLRV+DFGLSALTDQIRPDGLLHTLCGTPAYVAPE
Sbjct: 137 VGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 196

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLT GYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR
Sbjct: 197 ILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 256

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLI 300
           FLSRLLDTNP TRITVDEI +DPWF KG Q +EKL +EDS S++YGDV+N+KCLNAFDLI
Sbjct: 257 FLSRLLDTNPETRITVDEIHQDPWFVKGLQ-EEKLQIEDSGSEIYGDVNNAKCLNAFDLI 316

Query: 301 SFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVES 360
           SFSPGFDLTGL+NE E S+GVERF+SAE PEKII  VEE S+SE+AA VEKNGWGAKVE 
Sbjct: 317 SFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSENAA-VEKNGWGAKVEG 376

Query: 361 LDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           LDG F+V+I+IF+LTEELVVVEIK +  EVDQ+ QIWK  LRPQL+CL
Sbjct: 377 LDGGFIVVIDIFQLTEELVVVEIKNRVREVDQDGQIWKDILRPQLECL 422

BLAST of MC00g0476 vs. ExPASy TrEMBL
Match: A0A0A0L2V2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_4G331590 PE=3 SV=1)

HSP 1 Score: 690 bits (1780), Expect = 3.14e-248
Identity = 344/411 (83.70%), Postives = 378/411 (91.97%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIKAVNKLKV+ GG +AHVKREI
Sbjct: 18  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREI 77

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVME+A+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 78  AIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSKGRFSENLSRRYFQQLITA 137

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHSRGIFHRDLKPENLLLDDNFDLRV+DFGLSAL DQIRPDGLLHTLCGTPAYVAPE
Sbjct: 138 VGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIRPDGLLHTLCGTPAYVAPE 197

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDL+R
Sbjct: 198 ILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLQR 257

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQ--EKLHLEDSASDLYGDVSNSKCLNAFD 300
           F+SRLLDTNP +RITVDEI RDPWF KGFQ++  +   L+DSASD+YGDV+N+KCLNAFD
Sbjct: 258 FISRLLDTNPESRITVDEIQRDPWFVKGFQEEKFQFQELDDSASDVYGDVNNAKCLNAFD 317

Query: 301 LISFSPGFDLTGLLNEP-EISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAK 360
           LISFSPGFDLTGL+ E  E S+ VERF+SAE PEKII  VEEV++SE+AA VEKNGWG K
Sbjct: 318 LISFSPGFDLTGLIKEETEFSSCVERFLSAESPEKIIVTVEEVTKSENAA-VEKNGWGMK 377

Query: 361 VESLDGAFVVIINIFRLTEELVVVEIKKK--EVDQEFQIWKHKLRPQLDCL 406
           VE LDG FVV+I+IFRLTEELVVVEIK +  E++Q+ QIWK  LRPQL+CL
Sbjct: 378 VEGLDGGFVVVIDIFRLTEELVVVEIKNRVREIEQDRQIWKDVLRPQLECL 427

BLAST of MC00g0476 vs. ExPASy TrEMBL
Match: A0A5A7VJ38 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold624G00120 PE=3 SV=1)

HSP 1 Score: 685 bits (1768), Expect = 2.19e-246
Identity = 346/413 (83.78%), Postives = 375/413 (90.80%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIKAVNKLKV+ GG +AHVKREI
Sbjct: 18  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREI 77

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVME+A+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 78  AIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSKGRFSENLSRRYFQQLITA 137

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHSRGIFHRDLKPENLLLDDNFDLRV+DFGLSAL DQIRPDGLLHTLCGTPAYVAPE
Sbjct: 138 VGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIRPDGLLHTLCGTPAYVAPE 197

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLTAGYLPFNDSNLM+MYRKIYRGDFRCPKWTSPDL+R
Sbjct: 198 ILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMSMYRKIYRGDFRCPKWTSPDLQR 257

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLH----LEDSASDLYGDVSNSKCLNA 300
           FLSRLLDTNP TRITVDEI RDPWF KGFQ +EK      +ED AS++YGDV+NSK LNA
Sbjct: 258 FLSRLLDTNPETRITVDEIQRDPWFVKGFQ-EEKFQFQELIEDPASEVYGDVNNSKYLNA 317

Query: 301 FDLISFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGA 360
           FDLISFSPGFDLTGL+ E E S+ VERF+SAE PEKII +VEEV+ESE+AA VEKNGWG 
Sbjct: 318 FDLISFSPGFDLTGLIKETEFSSCVERFLSAESPEKIITRVEEVTESENAA-VEKNGWGV 377

Query: 361 KVESLDGAFVVIINIFRLTEELVVVEIKKK--EVDQEF-QIWKHKLRPQLDCL 406
           KVE LDG FVV+I+IFRLTEELVVVEIK +  E DQ+  Q WK  LRPQL+CL
Sbjct: 378 KVEGLDGGFVVVIDIFRLTEELVVVEIKNRVRETDQDGDQRWKDVLRPQLECL 428

BLAST of MC00g0476 vs. ExPASy TrEMBL
Match: A0A1S3CL72 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103502190 PE=3 SV=1)

HSP 1 Score: 685 bits (1768), Expect = 2.19e-246
Identity = 346/413 (83.78%), Postives = 375/413 (90.80%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           SLP+ ILFGKYELGKLLGCGAFAKVYH RN+ TG+SVAIKAVNKLKV+ GG +AHVKREI
Sbjct: 18  SLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREI 77

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           AIMRRLRHP+VVRL+EVLATKTKIYFVME+A+GGELF KVSKGRFSENLSRRYFQQLITA
Sbjct: 78  AIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSKGRFSENLSRRYFQQLITA 137

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           VGYCHSRGIFHRDLKPENLLLDDNFDLRV+DFGLSAL DQIRPDGLLHTLCGTPAYVAPE
Sbjct: 138 VGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIRPDGLLHTLCGTPAYVAPE 197

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           ILAKKGYDGAK+DVWSCGIILYVLTAGYLPFNDSNLM+MYRKIYRGDFRCPKWTSPDL+R
Sbjct: 198 ILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMSMYRKIYRGDFRCPKWTSPDLQR 257

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQDQEKLH----LEDSASDLYGDVSNSKCLNA 300
           FLSRLLDTNP TRITVDEI RDPWF KGFQ +EK      +ED AS++YGDV+NSK LNA
Sbjct: 258 FLSRLLDTNPETRITVDEIQRDPWFVKGFQ-EEKFQFQELIEDPASEVYGDVNNSKYLNA 317

Query: 301 FDLISFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGA 360
           FDLISFSPGFDLTGL+ E E S+ VERF+SAE PEKII +VEEV+ESE+AA VEKNGWG 
Sbjct: 318 FDLISFSPGFDLTGLIKETEFSSCVERFLSAESPEKIITRVEEVTESENAA-VEKNGWGV 377

Query: 361 KVESLDGAFVVIINIFRLTEELVVVEIKKK--EVDQEF-QIWKHKLRPQLDCL 406
           KVE LDG FVV+I+IFRLTEELVVVEIK +  E DQ+  Q WK  LRPQL+CL
Sbjct: 378 KVEGLDGGFVVVIDIFRLTEELVVVEIKNRVRETDQDGDQRWKDVLRPQLECL 428

BLAST of MC00g0476 vs. TAIR 10
Match: AT5G01820.1 (serine/threonine protein kinase 1 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.1e-154
Identity = 261/402 (64.93%), Postives = 326/402 (81.09%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREIAIMRRL 66
           LFGKYE+GKL+GCGAFAKVYH R+ ATG+SVAIK V+K ++  GG + +++REIAIM RL
Sbjct: 18  LFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMHRL 77

Query: 67  RHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHS 126
           RHP +VRL+EVLATK+KI+FVMEFA+GGELFAKVSKGRF E+LSRRYFQQLI+AVGYCHS
Sbjct: 78  RHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYCHS 137

Query: 127 RGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKKG 186
           RGIFHRDLKPENLLLD+  DL+++DFGLSALTDQIRPDGLLHTLCGTPAYVAPE+LAKKG
Sbjct: 138 RGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAKKG 197

Query: 187 YDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRLL 246
           YDGAK+D+WSCGIIL+VL AGYLPFND NLM MYRKIY+G+FR PKWTSPDLRR L+RLL
Sbjct: 198 YDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTRLL 257

Query: 247 DTNPATRITVDEILRDPWFSKGFQDQ-EKLHLEDSASDLYGDVSNSKC----LNAFDLIS 306
           DTNP TRIT++EI+ DPWF +G+ D+  K HLEDS   L  D ++S+     +NAFD+IS
Sbjct: 258 DTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDIIS 317

Query: 307 FSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVESL 366
            SPGF+L+GL  +      VERFVSA   E+++ ++EE+  +E+    +K  WG K+E  
Sbjct: 318 GSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIEGQ 377

Query: 367 DGAFVVIINIFRLTEELVVVEIKKKE--VDQEFQIWKHKLRP 402
            G F +++ I +LT+ELV++E++K++        +W   LRP
Sbjct: 378 KGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRP 419

BLAST of MC00g0476 vs. TAIR 10
Match: AT2G30360.1 (SOS3-interacting protein 4 )

HSP 1 Score: 479.9 bits (1234), Expect = 2.0e-135
Identity = 247/406 (60.84%), Postives = 317/406 (78.08%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVV-NGGCSAHVKREIAIMRR 66
           LFGKYELGKLLGCGAFAKV+HAR+  TG+SVA+K +NK K++ N   + ++KREI+IMRR
Sbjct: 17  LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRR 76

Query: 67  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSK-GRFSENLSRRYFQQLITAVGYC 126
           L HP++V+L+EV+ATK+KI+F MEF +GGELF K+SK GR SE+LSRRYFQQLI+AVGYC
Sbjct: 77  LSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYC 136

Query: 127 HSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAK 186
           H+RG++HRDLKPENLL+D+N +L+V+DFGLSALTDQIRPDGLLHTLCGTPAYVAPEIL+K
Sbjct: 137 HARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSK 196

Query: 187 KGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSR 246
           KGY+GAKVDVWSCGI+L+VL AGYLPFND N+M MY+KIY+G++R P+W SPDL+RF+SR
Sbjct: 197 KGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSR 256

Query: 247 LLDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASD--LYGDVSNSKCLNAFDLISF 306
           LLD NP TRIT+DEIL+DPWF +G   Q K H +D   D  +   +   K LNAFDLIS+
Sbjct: 257 LLDINPETRITIDEILKDPWFVRGGFKQIKFH-DDEIEDQKVESSLEAVKSLNAFDLISY 316

Query: 307 SPGFDLTGLLNEPEISAG-VERFVSAEPPEKIIGKVEEVSESESAAAVEK--NGWGAKVE 366
           S G DL+GL      S+G  ERF+S + PE +  +VE  +  E+    +K    +G ++E
Sbjct: 317 SSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEME 376

Query: 367 SLDGAFVVIINIFRLTEELVVVEIKKKEVDQEF--QIWKHKLRPQL 404
             +G F + I I RL + LVVVE +++  D +   ++W  KLR QL
Sbjct: 377 GQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQL 421

BLAST of MC00g0476 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 454.1 bits (1167), Expect = 1.2e-127
Identity = 234/443 (52.82%), Postives = 308/443 (69.53%), Query Frame = 0

Query: 1   SLPDPILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREI 60
           S P  ++ G+YE+GKLLG G FAKVY ARN+ T ESVAIK ++K KV+ GG  AH+KREI
Sbjct: 16  SSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREI 75

Query: 61  AIMRRLRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITA 120
           +I+RR+RHP++V+L+EV+ATK KIYFVME+ RGGELF KV+KGR  E ++R+YFQQLI+A
Sbjct: 76  SILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISA 135

Query: 121 VGYCHSRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPE 180
           V +CH+RG++HRDLKPENLLLD+N +L+V+DFGLSA++DQIR DGL HT CGTPAYVAPE
Sbjct: 136 VTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPE 195

Query: 181 ILAKKGYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRR 240
           +LA+KGYD AKVD+WSCG+IL+VL AGYLPF+D N+MAMY+KIYRG+FRCP+W S +L R
Sbjct: 196 VLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTR 255

Query: 241 FLSRLLDTNPATRITVDEILRDPWFSKGFQ---------------DQEKLHLEDSASDLY 300
            LS+LL+TNP  R T  EI+ + WF KGF+               D ++L  +   SD  
Sbjct: 256 LLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRD 315

Query: 301 GDVSNSK-----------------CLNAFDLISFSPGFDLTGLLNEPEISAGVERFVSAE 360
              S S+                  LNAFD+ISFS GFDL+GL ++        RFVS  
Sbjct: 316 SAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDD---DGEGSRFVSGA 375

Query: 361 PPEKIIGKVEEVSESESAAAVEKN---GWGAKVESLDGAFVVIINIFRLTEELVVVEIKK 407
           P  KII K+EE+++  S    +K+         + + G   +   IF LT  LVVVE+KK
Sbjct: 376 PVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKK 435

BLAST of MC00g0476 vs. TAIR 10
Match: AT2G38490.1 (CBL-interacting protein kinase 22 )

HSP 1 Score: 448.0 bits (1151), Expect = 8.2e-126
Identity = 227/398 (57.04%), Postives = 293/398 (73.62%), Query Frame = 0

Query: 7   LFGKYELGKLLGCGAFAKVYHARNIAT-GESVAIKAVNKLKVVNGGCSAHVKREIAIMRR 66
           LFGKY+LGKLLG GAFAKVY A ++   GESVAIK V K K +  G +AHVKREI++MRR
Sbjct: 48  LFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK-KRLKDGLTAHVKREISVMRR 107

Query: 67  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 126
           LRHPH+V L EVLATKTKIYFVME A+GGELF++V+  RF+E+LSR+YF+QLI+AV YCH
Sbjct: 108 LRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNRFTESLSRKYFRQLISAVRYCH 167

Query: 127 SRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 186
           +RG+FHRDLKPENLLLD+N DL+V+DFGLSA+ +QI PDG+LHTLCGTPAYVAPE+L KK
Sbjct: 168 ARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPDGMLHTLCGTPAYVAPELLLKK 227

Query: 187 GYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRL 246
           GYDG+K D+WSCG++L++L AGYLPF D N+M +YRKI++  ++ P WTS DLR+ L RL
Sbjct: 228 GYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIHKAQYKLPDWTSSDLRKLLRRL 287

Query: 247 LDTNPATRITVDEILRDPWFSKGFQDQEKLHLEDSASDLYGDVSNSKCLNAFDLISFSPG 306
           L+ NP  RITV+EIL+DPWF+ G    E + ++   +D Y    N K LNAFDLIS +  
Sbjct: 288 LEPNPELRITVEEILKDPWFNHGVDPSEIIGIQ---ADDYDLEENGKILNAFDLISSASS 347

Query: 307 FDLTGLLNEPEISAGVERFVSAEPPEKIIGKVEEVSESESAAAVEKNGWGAKVESLDGAF 366
            +L+GL          ++FVS E    I+ KVEEV++  +    +K     K+E   G  
Sbjct: 348 SNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVAKQLNLRIAKKKERAIKLEGPHGVA 407

Query: 367 VVIINIFRLTEELVVVEIKKKEVDQEFQIWKHKLRPQL 404
            V++ + RLT ELV+VE+K K+ D    +W   LR +L
Sbjct: 408 NVVVKVRRLTNELVMVEMKNKQRDVGL-VWADALRQKL 440

BLAST of MC00g0476 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 446.0 bits (1146), Expect = 3.1e-125
Identity = 234/437 (53.55%), Postives = 301/437 (68.88%), Query Frame = 0

Query: 6   ILFGKYELGKLLGCGAFAKVYHARNIATGESVAIKAVNKLKVVNGGCSAHVKREIAIMRR 65
           IL GKYELGKLLG G FAKVY A+NI +G+ VAIK ++K K++  G  AH+KREI+I+RR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 66  LRHPHVVRLYEVLATKTKIYFVMEFARGGELFAKVSKGRFSENLSRRYFQQLITAVGYCH 125
           +RHP++V L+EV+ATK+KIYFVME+  GGELF  V+KGR  E  +RRYFQQLI++V +CH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 126 SRGIFHRDLKPENLLLDDNFDLRVTDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILAKK 185
            RG++HRDLKPENLLLD+  +L+V+DFGLSA+ +Q+R DGL HT CGTPAY+APE+L +K
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 186 GYDGAKVDVWSCGIILYVLTAGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRL 245
           GYD AK DVWSCG+IL+VL AG++PF D N+M MY+KIY+G+FRCP+W S DL R L+RL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 246 LDTNPATRITVDEILRDPWFSKGFQ------DQEKLHLED----------------SASD 305
           LDTNP TRIT+ EI+++ WF KGF+      + +KL  ED                S SD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 306 L--------YGDVSNSKCLNAFDLISFSPGFDLTGLLNEPEISAGVERFVSAEPPEKIIG 365
                     G +     LNAFD+ISFS GFDL+GL  E E   G  RFVS  P  KII 
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE-EGGEGT-RFVSGAPVSKIIS 428

Query: 366 KVEEVSESESAAAVEKNGWGAKV----ESLDGAFVVIINIFRLTEELVVVEIKKKEVDQE 407
           K+EE+++  S   V K  W  ++    E   G   +   IF LT  LVVVE+KKK  D+E
Sbjct: 429 KLEEIAKIVS-FTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDRE 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZW41.6e-15364.93CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana OX=37... [more]
O229322.8e-13460.84CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana OX=37... [more]
Q9SN431.6e-12652.82CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=37... [more]
Q68Y498.9e-12551.67CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
O809021.2e-12457.04CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
KAG7033738.11.39e-25485.78CBL-interacting serine/threonine-protein kinase 14, partial [Cucurbita argyrospe... [more]
XP_022949666.13.07e-25485.54CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata][more]
XP_023545198.13.57e-25385.29CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. p... [more]
XP_022978189.13.57e-25385.29CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita maxima][more]
XP_038883854.14.12e-25083.58CBL-interacting serine/threonine-protein kinase 14 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GCM31.48e-25485.54Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1ITF41.73e-25385.29Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0L2V23.14e-24883.70Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_4... [more]
A0A5A7VJ382.19e-24683.78Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CL722.19e-24683.78Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
Match NameE-valueIdentityDescription
AT5G01820.11.1e-15464.93serine/threonine protein kinase 1 [more]
AT2G30360.12.0e-13560.84SOS3-interacting protein 4 [more]
AT4G18700.11.2e-12752.82CBL-interacting protein kinase 12 [more]
AT2G38490.18.2e-12657.04CBL-interacting protein kinase 22 [more]
AT1G29230.13.1e-12553.55CBL-interacting protein kinase 18 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 11..265
e-value: 2.1E-108
score: 376.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 11..265
e-value: 1.9E-71
score: 240.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 11..265
score: 51.862606
IPR004041NAF domainPFAMPF03822NAFcoord: 292..345
e-value: 4.9E-17
score: 61.8
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 295..406
e-value: 6.4E-29
score: 102.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 5..294
e-value: 2.7E-102
score: 344.4
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 15..218
e-value: 2.6E-12
score: 44.5
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 12..260
e-value: 4.5E-14
score: 50.0
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 6..404
NoneNo IPR availablePANTHERPTHR43895:SF22CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 14coord: 6..404
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 295..404
e-value: 1.76709E-34
score: 121.91
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 17..40
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 289..313
score: 11.410439
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 7..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC00g0476.1MC00g0476.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity