MC00g0258 (gene) Bitter gourd (Dali-11) v1

BLAST of MC00g0258 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.8e-182
Identity = 349/596 (58.56%), Postives = 413/596 (69.30%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           D P  SSY+ AMEALS+LI ++ RG++     N +KLE +  Y++ILDLE  +++LK+IH
Sbjct: 76  DNPALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIH 135

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTC F EAILR CG RTG+FTSPHLIDVRERFRI+GLDISE+KFLQYFW CW
Sbjct: 136 VAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECW 195

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
             LKE   + L MP LFQFLT+LAF IF  E+VDVA+IEVGLGG  DSTNVI++PVVCGI
Sbjct: 196 KLLKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGI 255

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
            SLGMDHM+ L                                  GN+L  IA HKAGI 
Sbjct: 256 ASLGMDHMDIL----------------------------------GNTLADIAFHKAGIF 315

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KPQ+PAFTVPQL EAMDV+QK A  L +PLEV  PL+ K+LDG+ LG+SGDHQLVN+GLA
Sbjct: 316 KPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKKLDGVTLGLSGDHQLVNAGLA 375

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSL +CWLQRTGNW++IF N  K   +P AF RGLATA L GRAQ+V+D  +   +S+  
Sbjct: 376 VSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARLHGRAQVVHDVVSDPQDSSDS 435

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
            E   GDL+FYLDGAHSPESMEAC  WFSS V+G+ KS    +   +       DL    
Sbjct: 436 METPCGDLIFYLDGAHSPESMEACGRWFSSAVRGD-KSLSTAVNGYMRHGEYGTDL---- 495

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
                 +VSKQILLFNCMEVRDPQ+LLP+LV TCASSG +FS+ALFVPSMSTY KV S  
Sbjct: 496 -----NRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFSRALFVPSMSTYNKVISGA 555

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERML-SHLYEDSHSSLS 542
           S   SD   KDL+WQF LQR+WEK + G   DA  D  LK D    L  H +    +   
Sbjct: 556 SAIPSDTRRKDLTWQFRLQRLWEKSIQG--TDAGLDHTLKPDGITALPPHDFLCGDAPQC 615

Query: 543 DNNFG----DSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 594
               G     SAVMPSLPL I WLRDCVR +PS++L+VLVTGSLH+VGDVL+LL+R
Sbjct: 616 GGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 625

BLAST of MC00g0258 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.5e-144
Identity = 293/586 (50.00%), Postives = 378/586 (64.51%), Query Frame = 0

Query: 9   SYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGTKG 68
           SYE A+ ALSSLI ++ R +K   ++  ++ E++  Y+++LDLE+ + K+ +IHVAGTKG
Sbjct: 66  SYEEALAALSSLITKRSRADK---SNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKG 125

Query: 69  KGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLKEN 128
           KGSTCTF E+I+R  G RTGLFTSPHLIDVRERFR++G+DISE+KFL YFW C+ +LKE 
Sbjct: 126 KGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKER 185

Query: 129 VTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLGMD 188
             E +PMP  F+FL LLAF IF+ E+VD AI+EVGLGG +D+TN +++PVVCGI+SLG D
Sbjct: 186 TNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYD 245

Query: 189 HMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQVPA 248
           HME L                                  G++LG IA  KAGI K  VPA
Sbjct: 246 HMEIL----------------------------------GDTLGKIAGEKAGIFKLGVPA 305

Query: 249 FTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLCKC 308
           FTVPQ  EAM V+++KA    + LEV +PL  + L G KLG+ G+HQ VN+GLAVSL   
Sbjct: 306 FTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASI 365

Query: 309 WLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESENSNG 368
           WLQ+ G  +    +  +++ +P+ F++GLATA+L GRAQ+V D+Y  S  S        G
Sbjct: 366 WLQQIGKLE--VPSRTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTS--------G 425

Query: 369 DLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEAESK 428
           DLVFYLDGAHSPESMEACA WFS  VKG+++S       N    S +D          S 
Sbjct: 426 DLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHDKW--------SN 485

Query: 429 KVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVSSSD 488
           +  +QILLFNCM VRDP +LLP L N CA  GVNF KALFVP+MS Y KV ++  +  +D
Sbjct: 486 ETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPEND 545

Query: 489 IMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNFGDS 548
               DLSWQF LQ++WE ++        S++D + D E              SD   G+S
Sbjct: 546 -PQVDLSWQFTLQKVWESLVQ-------SERDGEKDGE--------------SD---GNS 571

Query: 549 AVMPSLPLAIKWLRDCVRESPS-VRLQVLVTGSLHMVGDVLKLLRR 594
            V  SLP+AIK LRD V ES S  R QVLVTGSLH+VGDVL+L+R+
Sbjct: 606 EVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIRK 571

BLAST of MC00g0258 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 266/588 (45.24%), Postives = 356/588 (60.54%), Query Frame = 0

Query: 7   SSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGT 66
           S+ Y++ ++ALSSLI ++ R   +   +   +  ++  Y+++L+LE  V ++KIIHVAGT
Sbjct: 10  SARYQNTLDALSSLITKRGR---LASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGT 69

Query: 67  KGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLK 126
           KGKGSTCTF E+ILR  GLRTGLFTSPHLIDVRERFR+NG++IS++KF+ YFW C+ +LK
Sbjct: 70  KGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLK 129

Query: 127 ENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLG 186
           E  +  +PMP  F FL LLAF IF+ EQVDV I+EVGLGG +D+TNVI++PVVCGI+SLG
Sbjct: 130 EKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLG 189

Query: 187 MDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQV 246
            DHME LG                                   +L  IA+ KAGI K  V
Sbjct: 190 YDHMEILGY----------------------------------TLAEIAAEKAGIFKSGV 249

Query: 247 PAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLC 306
           PAFTV Q  EAM V+ +KA  L + L+V EPLD  +    +LG+ G+HQ +N+GLAV+LC
Sbjct: 250 PAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALC 309

Query: 307 KCWLQRTGNWQRIFQNGFKVAN-MPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESEN 366
             +L+  G   +   NG    N +P+ F+ GL+ A L GRA IV D       S +  E 
Sbjct: 310 STFLKEIGIEDK---NGLDQTNGLPEKFISGLSNAYLMGRAMIVPD-------SELPEE- 369

Query: 367 SNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEA 426
               +V+YLDGAHSPESMEACA WFS  +K N                          + 
Sbjct: 370 ----IVYYLDGAHSPESMEACAIWFSKQIKQN--------------------------QE 429

Query: 427 ESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVS 486
            ++K S+QILLFNCM VRDP +LLPRL + C   GV+F +A+FVP++S Y +V SS +V 
Sbjct: 430 RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVG 489

Query: 487 SSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNF 546
           +     + +SWQF LQRIWE +  G+                  S+   DS     + +F
Sbjct: 490 T---RVESMSWQFGLQRIWESLARGEAK----------------SNSKSDSKGKEEEKSF 492

Query: 547 GDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 594
               V  SLP+A+ WLRD  R+S  VR QVLVTGSLH+VGD+L+ +++
Sbjct: 550 ----VFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 492

BLAST of MC00g0258 vs. ExPASy Swiss-Prot
Match: Q924L9 (Folylpolyglutamate synthase, mitochondrial OS=Cricetulus griseus OX=10029 GN=FPGS PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.1e-66
Identity = 200/631 (31.70%), Postives = 296/631 (46.91%), Query Frame = 0

Query: 10  YESAMEALSSLIKQKRRGEKVLGTSND--EKLEMMRKYVEILDLE-QGVRKLKIIHVAGT 69
           Y+ A+  L++L       E+V    +D   +LE M  Y+    L+ + + +L IIHV GT
Sbjct: 45  YQDAVRMLNTLQTNASYLEQVKRQRSDPQAQLEAMEVYLARSGLQVEDLNQLNIIHVTGT 104

Query: 70  KGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLK 129
           KGKGSTC F E ILR  GL+TG F+SPHL+ VRER RING  IS + F ++FW  + QL+
Sbjct: 105 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGKPISPELFTKHFWRLYHQLE 164

Query: 130 E-NVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSL 189
           E     ++ MPA F+FLTL+AF +F  E+VD+A++EVG+GG YD TN+I++PVVCG++SL
Sbjct: 165 EFKDDSHVAMPAYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSL 224

Query: 190 GMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQ 249
           GMDH   L                                  G+++  IA  K GI KP 
Sbjct: 225 GMDHTSLL----------------------------------GDTVEKIAWQKGGIFKPG 284

Query: 250 VPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDG--LKLGMSGDHQLVNSGLAV 309
           VPAFTV Q    + V++ +A+    PL +  PL+     G  L LG+ G+HQ  N+ LA+
Sbjct: 285 VPAFTVLQPEGPLAVLRDRAQQTSCPLYLCPPLEALEDGGLPLTLGLEGEHQRSNAALAL 344

Query: 310 SLCKCWLQRTGN--------------WQRIFQNGFKVANMPDAFLR-GLATANLSGRAQI 369
            L  CWL++  +              WQ      F     P + +R GL      GR Q+
Sbjct: 345 QLAHCWLEQQDHQDIRELKVSRPSMRWQLPLAPVFH----PTSHMRHGLRDTEWPGRTQV 404

Query: 370 VYDRYASSHNSTMESENSNGDLVFYLDGAHSPESMEACANWF-SSVVKGNHKS--PHCFM 429
           +                  G L +YLDGAH+  S++AC  W+  S+ +  H S  P  + 
Sbjct: 405 L----------------RRGPLTWYLDGAHTTSSVQACVRWYCQSLQRSKHPSGGPEVY- 464

Query: 430 AKNIDRDSENDDLVKVMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSK 489
                                       +LLFN    RD   LL +L+  C      F  
Sbjct: 465 ----------------------------VLLFNSTGDRDSAALL-KLLQPC-----QFDY 524

Query: 490 ALFVPSMSTYTKVTSSNSVSSSDIMSKDLSWQFNLQRIWEKIMHGK-EPDAVSDKDLKLD 549
           A+F P+++  +   +++  + +  + + L      Q+ W  +   +  PD +S    +  
Sbjct: 525 AVFCPNLTEVSSTENADQQNFTVTLDQVLLRCLQHQQHWSCLSEKQASPDFLSSPSPEPG 581

Query: 550 SERMLSHLYEDSHSSLSDNNFGDSAVMPSLPLAIKWL---RDCVRESPSV---------- 592
               L       HS+ +     +S V   +  A++W+   RD + ++PS           
Sbjct: 585 RPGSLQPALRPPHSTGT-----NSLVFSCISHALQWISQGRDPIFQAPSPPRSLLSHPTA 581

BLAST of MC00g0258 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.0e-66
Identity = 200/629 (31.80%), Postives = 298/629 (47.38%), Query Frame = 0

Query: 10  YESAMEALSSLIKQKRRGEKVLGTSND--EKLEMMRKYVEILDLE-QGVRKLKIIHVAGT 69
           Y+ A+  L++L       E V     D   +LE M+ Y+    L+ + + +L IIH+ GT
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101

Query: 70  KGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLK 129
           KGKGSTC F E ILR  GL+TG F+SPHL+ VRER RING  IS + F ++FW  + +L+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161

Query: 130 E--NVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITS 189
           E  + +  + MP  F+FLTL+AF +F  E+VD+A++EVG+GG YD TN+I++PVVCGITS
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221

Query: 190 LGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKP 249
           LG+DH   L                                  G+++  IA  K GI K 
Sbjct: 222 LGIDHTGLL----------------------------------GDTMEKIAWQKGGIFKS 281

Query: 250 QVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDG--LKLGMSGDHQLVNSGLA 309
            VPAFTV Q  E + V++ +A+ +  PL +  PL      G  L LG+ G+HQ  N+ LA
Sbjct: 282 GVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPLQALEEGGPPLTLGLEGEHQRSNAALA 341

Query: 310 VSLCKCWLQRTGNWQRIFQNGFKVANMP------------DAFLRGLATANLSGRAQIVY 369
           + + +CWLQ+ G +Q   +      ++P                +GL      GR Q++ 
Sbjct: 342 LQVARCWLQQKG-YQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEWPGRTQLL- 401

Query: 370 DRYASSHNSTMESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNID 429
                            G L +YLDGAH+  SM+AC  WF   +   H+       +  D
Sbjct: 402 ---------------RRGPLTWYLDGAHTTSSMQACVRWFRQAL---HR------CERPD 461

Query: 430 RDSENDDLVKVMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVP 489
             SE                  ++LLFN    RD   LL +L+  C      F  A+F P
Sbjct: 462 SGSE-----------------VRVLLFNSTGDRDSAALL-KLLQPC-----QFDYAIFCP 521

Query: 490 SMSTYTKVTSSN----SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSE 549
           +++      +++    +V+   ++ + L+ Q +  R+ E+ +    PD  S    + D  
Sbjct: 522 NLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQV---SPDPWSTPGQEQDGP 579

Query: 550 RMLSHLYEDSHSSLSDNNFGDSAVMPSLPLAIKWL---RDCVRESPS------------- 592
             L       H+     +   S V   +  A++W+   RD + + PS             
Sbjct: 582 ASLLLAPHPPHT-----HSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADS 579

BLAST of MC00g0258 vs. NCBI nr
Match: XP_022158588.1 (folylpolyglutamate synthase [Momordica charantia])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 559/593 (94.27%), Postives = 559/593 (94.27%), Query Frame = 0

Query: 1   LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI 60
           LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI
Sbjct: 11  LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI 70

Query: 61  IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA 120
           IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA
Sbjct: 71  IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA 130

Query: 121 CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC 180
           CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC
Sbjct: 131 CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC 190

Query: 181 GITSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAG 240
           GITSLGMDHMETLG                                  NSLGMIASHKAG
Sbjct: 191 GITSLGMDHMETLG----------------------------------NSLGMIASHKAG 250

Query: 241 ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG 300
           ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG
Sbjct: 251 ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG 310

Query: 301 LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST 360
           LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST
Sbjct: 311 LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST 370

Query: 361 MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK 420
           MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK
Sbjct: 371 MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK 430

Query: 421 VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS 480
           VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS
Sbjct: 431 VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS 490

Query: 481 SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL 540
           SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL
Sbjct: 491 SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL 550

Query: 541 SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR
Sbjct: 551 SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 569

BLAST of MC00g0258 vs. NCBI nr
Match: XP_022983741.1 (folylpolyglutamate synthase isoform X1 [Cucurbita maxima] >XP_022983742.1 folylpolyglutamate synthase isoform X1 [Cucurbita maxima] >XP_022983743.1 folylpolyglutamate synthase isoform X1 [Cucurbita maxima])

HSP 1 Score: 924 bits (2388), Expect = 0.0
Identity = 472/591 (79.86%), Postives = 513/591 (86.80%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESA+EALSSLI QK RG  V    +  KLE MRKYVEILDLE+ V+KLKIIH
Sbjct: 4   NLPQSSSYESALEALSSLITQKSRGAMVPVIRSKTKLERMRKYVEILDLEECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFWACW
Sbjct: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QLKE+VTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGGMYDSTNVI+EP+VCGI
Sbjct: 124 RQLKESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSL  IASHKAGIL
Sbjct: 184 TSLGMDHMELLG----------------------------------NSLSQIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KP+VPAFTVPQLPEAMDVIQKKAR LMIPLEVAEPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPRVPAFTVPQLPEAMDVIQKKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFK A +PDAFLRGL+TANLSGRAQIVYD YASS NS+ +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKAAKLPDAFLRGLSTANLSGRAQIVYDCYASSFNSSTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           SENSNGDL+FYLDGAHSPESM ACANWFSSVVKGNHKS HCF A+N+DRDS NDD VKV+
Sbjct: 364 SENSNGDLIFYLDGAHSPESMGACANWFSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K SKQILLFNCME+RDP+IL PRLVNTCASSGV+FSKALFVP+MSTY+KV+SSN
Sbjct: 424 QEAESTKFSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQFNLQ++WEKIMHGKEPD++S+KDLKLD ER L  LYE+ HSSLSD
Sbjct: 484 SVTSSDIINKDLSWQFNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEEFHSSLSD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N F  SAVMPSLPLAI WLRDCVRESPS+RLQVLVTGSLH+VGDVLKL++R
Sbjct: 544 NKFSGSAVMPSLPLAINWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLIKR 559

BLAST of MC00g0258 vs. NCBI nr
Match: XP_038904563.1 (folylpolyglutamate synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 473/591 (80.03%), Postives = 513/591 (86.80%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESAMEALSSLI QKRRGE V    N+EKLE MRKYVEILD+E+ V+KLKIIH
Sbjct: 4   NLPQSSSYESAMEALSSLITQKRRGEMVSAIRNEEKLERMRKYVEILDIEECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTFCEAILRECGL+TGLFTSPHLIDVRERFR+NGL+ISEDKFL+YFWACW
Sbjct: 64  VAGTKGKGSTCTFCEAILRECGLQTGLFTSPHLIDVRERFRVNGLEISEDKFLRYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QLKENVTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGG YDSTNVI+EP+VCGI
Sbjct: 124 TQLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSLGMIASHKAGIL
Sbjct: 184 TSLGMDHMEILG----------------------------------NSLGMIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KPQVPAFTVPQ PEAMDVIQKKAR LMIPLEV EPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPQVPAFTVPQFPEAMDVIQKKARELMIPLEVTEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFK A +P AFL+GL+TANLSGRAQIVYD Y SS NST +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKGAKIPAAFLKGLSTANLSGRAQIVYDCYTSSFNSTTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           S+NSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKS +CF AKN+DRDS +DD VKV+
Sbjct: 364 SDNSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSLNCFRAKNLDRDSGSDDPVKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K+SKQILLFNCMEVRDP ILLPRLVNTCASSGV+FSKALFVP+MSTY+KV+S N
Sbjct: 424 QEAESTKLSKQILLFNCMEVRDPHILLPRLVNTCASSGVHFSKALFVPTMSTYSKVSSGN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQ NLQR+WEKIMHGK  DA+ +KDLKLDSER L  LYE+S SSL D
Sbjct: 484 SVTSSDIINKDLSWQLNLQRLWEKIMHGK--DAIFEKDLKLDSERGLPALYEESLSSLLD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           NN   SAVMPSLPLAIK LRDCVRESPS+RLQVLVTGSLH+VGDVLKLL+R
Sbjct: 544 NNLSGSAVMPSLPLAIKLLRDCVRESPSIRLQVLVTGSLHLVGDVLKLLKR 558

BLAST of MC00g0258 vs. NCBI nr
Match: XP_023527435.1 (folylpolyglutamate synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527436.1 folylpolyglutamate synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527437.1 folylpolyglutamate synthase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 469/591 (79.36%), Postives = 510/591 (86.29%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESA+EALSSLI QK RG       +  KLE MRKYVEILDL + V+KLKIIH
Sbjct: 4   NLPQSSSYESALEALSSLITQKSRGTMAPVIRSKTKLERMRKYVEILDLVECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFWACW
Sbjct: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QL E+VTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGGMYDSTNVI+EP+VCGI
Sbjct: 124 RQLTESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSL  IASHKAGIL
Sbjct: 184 TSLGMDHMELLG----------------------------------NSLSQIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KP+VPAFTVPQLPEAMDVIQ KAR LMIPLEVAEPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPRVPAFTVPQLPEAMDVIQNKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFKVA +PDAFLRGL+TANLSGRAQ VYD YASS NS+ +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           SENSNGDL+FYLDGAHSPESMEACANWFSSVVKGNHKS HCF A+N+DRDS NDD VKV+
Sbjct: 364 SENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K+SKQILLFNCME+RDP+IL PRLVNTCASSGV+FSKALFVP+MSTY+KV+SSN
Sbjct: 424 QEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQ NLQ++WEKIMHGKEPD++S+KDLKLD ER L  LYED HSSLSD
Sbjct: 484 SVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEDFHSSLSD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N F  SAVMPSLPLAI WLRDCVRESPS+RLQVLVTGSLH+VGDVLKL++R
Sbjct: 544 NKFSGSAVMPSLPLAINWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLIKR 559

BLAST of MC00g0258 vs. NCBI nr
Match: XP_022934382.1 (folylpolyglutamate synthase isoform X1 [Cucurbita moschata] >XP_022934383.1 folylpolyglutamate synthase isoform X1 [Cucurbita moschata] >XP_022934384.1 folylpolyglutamate synthase isoform X1 [Cucurbita moschata])

HSP 1 Score: 917 bits (2370), Expect = 0.0
Identity = 468/591 (79.19%), Postives = 510/591 (86.29%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESA+EALSSLI QK RG       +  KLE MRKY+EILDL + V+KLKIIH
Sbjct: 4   NLPQSSSYESALEALSSLITQKSRGAMTPVIRSKTKLERMRKYIEILDLVECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFWACW
Sbjct: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QLKE+VTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGGMYDSTNVI+EP+VCGI
Sbjct: 124 RQLKESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSL  IASHKAGIL
Sbjct: 184 TSLGMDHMELLG----------------------------------NSLSQIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KP+VPAFTVPQLPEAMDVIQ KAR LMIPLEVAEPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPRVPAFTVPQLPEAMDVIQNKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFKVA +PDAFLRGL+TANLSGRAQ VYD YASS NS+ +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           SENSNGDL+FYLDGAHSPESMEACANWFSSVVKGNHKS HCF A+N+DRDS  DD VKV+
Sbjct: 364 SENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLHCFSAENVDRDSGKDD-VKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K+SKQILLFNCME+RDP+IL PRLVNTCASSGV+FSKALFVP+MSTY+KV+SSN
Sbjct: 424 QEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQ NLQ++WEKIMHGKEPD++S+KDLKLD ER L  LYED HSSLSD
Sbjct: 484 SVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEDFHSSLSD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N F  SAVMPSLPLAI WLRDCVRESPS+RLQVLVTGSLH+VGDVLKL++R
Sbjct: 544 NKFSGSAVMPSLPLAINWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLIKR 559

BLAST of MC00g0258 vs. ExPASy TrEMBL
Match: A0A6J1DWI5 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111025044 PE=3 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 559/593 (94.27%), Postives = 559/593 (94.27%), Query Frame = 0

Query: 1   LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI 60
           LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI
Sbjct: 11  LKDLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKI 70

Query: 61  IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA 120
           IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA
Sbjct: 71  IHVAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWA 130

Query: 121 CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC 180
           CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC
Sbjct: 131 CWIQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVC 190

Query: 181 GITSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAG 240
           GITSLGMDHMETLG                                  NSLGMIASHKAG
Sbjct: 191 GITSLGMDHMETLG----------------------------------NSLGMIASHKAG 250

Query: 241 ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG 300
           ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG
Sbjct: 251 ILKPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSG 310

Query: 301 LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST 360
           LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST
Sbjct: 311 LAVSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNST 370

Query: 361 MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK 420
           MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK
Sbjct: 371 MESENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVK 430

Query: 421 VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS 480
           VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS
Sbjct: 431 VMQEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTS 490

Query: 481 SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL 540
           SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL
Sbjct: 491 SNSVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSL 550

Query: 541 SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR
Sbjct: 551 SDNNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 569

BLAST of MC00g0258 vs. ExPASy TrEMBL
Match: A0A6J1J8K8 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482270 PE=3 SV=1)

HSP 1 Score: 924 bits (2388), Expect = 0.0
Identity = 472/591 (79.86%), Postives = 513/591 (86.80%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESA+EALSSLI QK RG  V    +  KLE MRKYVEILDLE+ V+KLKIIH
Sbjct: 4   NLPQSSSYESALEALSSLITQKSRGAMVPVIRSKTKLERMRKYVEILDLEECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFWACW
Sbjct: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QLKE+VTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGGMYDSTNVI+EP+VCGI
Sbjct: 124 RQLKESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSL  IASHKAGIL
Sbjct: 184 TSLGMDHMELLG----------------------------------NSLSQIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KP+VPAFTVPQLPEAMDVIQKKAR LMIPLEVAEPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPRVPAFTVPQLPEAMDVIQKKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFK A +PDAFLRGL+TANLSGRAQIVYD YASS NS+ +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKAAKLPDAFLRGLSTANLSGRAQIVYDCYASSFNSSTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           SENSNGDL+FYLDGAHSPESM ACANWFSSVVKGNHKS HCF A+N+DRDS NDD VKV+
Sbjct: 364 SENSNGDLIFYLDGAHSPESMGACANWFSSVVKGNHKSLHCFSAENVDRDSGNDD-VKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K SKQILLFNCME+RDP+IL PRLVNTCASSGV+FSKALFVP+MSTY+KV+SSN
Sbjct: 424 QEAESTKFSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQFNLQ++WEKIMHGKEPD++S+KDLKLD ER L  LYE+ HSSLSD
Sbjct: 484 SVTSSDIINKDLSWQFNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEEFHSSLSD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N F  SAVMPSLPLAI WLRDCVRESPS+RLQVLVTGSLH+VGDVLKL++R
Sbjct: 544 NKFSGSAVMPSLPLAINWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLIKR 559

BLAST of MC00g0258 vs. ExPASy TrEMBL
Match: A0A6J1F7I2 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441573 PE=3 SV=1)

HSP 1 Score: 917 bits (2370), Expect = 0.0
Identity = 468/591 (79.19%), Postives = 510/591 (86.29%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           +LPQSSSYESA+EALSSLI QK RG       +  KLE MRKY+EILDL + V+KLKIIH
Sbjct: 4   NLPQSSSYESALEALSSLITQKSRGAMTPVIRSKTKLERMRKYIEILDLVECVKKLKIIH 63

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTCTF EAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFL YFWACW
Sbjct: 64  VAGTKGKGSTCTFSEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLHYFWACW 123

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
            QLKE+VTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGGMYDSTNVI+EP+VCGI
Sbjct: 124 RQLKESVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGMYDSTNVIEEPIVCGI 183

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
           TSLGMDHME LG                                  NSL  IASHKAGIL
Sbjct: 184 TSLGMDHMELLG----------------------------------NSLSQIASHKAGIL 243

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KP+VPAFTVPQLPEAMDVIQ KAR LMIPLEVAEPLDHK+LDGLKLGMSGDHQLVNSGLA
Sbjct: 244 KPRVPAFTVPQLPEAMDVIQNKARELMIPLEVAEPLDHKKLDGLKLGMSGDHQLVNSGLA 303

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSLCKCWLQRTGNW+R+F NGFKVA +PDAFLRGL+TANLSGRAQ VYD YASS NS+ +
Sbjct: 304 VSLCKCWLQRTGNWERMFLNGFKVAKIPDAFLRGLSTANLSGRAQTVYDCYASSFNSSTQ 363

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
           SENSNGDL+FYLDGAHSPESMEACANWFSSVVKGNHKS HCF A+N+DRDS  DD VKV+
Sbjct: 364 SENSNGDLIFYLDGAHSPESMEACANWFSSVVKGNHKSLHCFSAENVDRDSGKDD-VKVL 423

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
           QEAES K+SKQILLFNCME+RDP+IL PRLVNTCASSGV+FSKALFVP+MSTY+KV+SSN
Sbjct: 424 QEAESTKLSKQILLFNCMEMRDPEILFPRLVNTCASSGVHFSKALFVPTMSTYSKVSSSN 483

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSD 542
           SV+SSDI++KDLSWQ NLQ++WEKIMHGKEPD++S+KDLKLD ER L  LYED HSSLSD
Sbjct: 484 SVTSSDIINKDLSWQLNLQKLWEKIMHGKEPDSISEKDLKLDHERGLPPLYEDFHSSLSD 543

Query: 543 NNFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N F  SAVMPSLPLAI WLRDCVRESPS+RLQVLVTGSLH+VGDVLKL++R
Sbjct: 544 NKFSGSAVMPSLPLAINWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLIKR 559

BLAST of MC00g0258 vs. ExPASy TrEMBL
Match: A0A0A0LHM9 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G823100 PE=3 SV=1)

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 466/590 (78.98%), Postives = 503/590 (85.25%), Query Frame = 0

Query: 4   LPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHV 63
           LPQ  SYES MEALSSLI QKRRGE V    N+EK   MRKYVEIL +E+ V+KLKIIHV
Sbjct: 5   LPQLPSYESTMEALSSLITQKRRGEMVSAIRNEEKSMRMRKYVEILGIEECVKKLKIIHV 64

Query: 64  AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWI 123
           AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 
Sbjct: 65  AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWT 124

Query: 124 QLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGIT 183
           QLKENVTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGG YDSTNVI+EP+VCGIT
Sbjct: 125 QLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIVCGIT 184

Query: 184 SLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILK 243
           SLGMDHME LG                                  +SLGMIASHKAGILK
Sbjct: 185 SLGMDHMEILG----------------------------------DSLGMIASHKAGILK 244

Query: 244 PQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAV 303
           PQVPAFTVPQLPEAMDVIQKKAR LM+PLEVAEPL+ K+LDGLKLGMSGDHQ VNSGLAV
Sbjct: 245 PQVPAFTVPQLPEAMDVIQKKARELMVPLEVAEPLNDKKLDGLKLGMSGDHQFVNSGLAV 304

Query: 304 SLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTMES 363
           SLCKCWLQRTGNW+R+  NG K A +P AFLRGL+TANLSGRAQ VYD Y SS NST +S
Sbjct: 305 SLCKCWLQRTGNWERMLLNGCKGAKIPAAFLRGLSTANLSGRAQTVYDYYTSSFNSTTQS 364

Query: 364 ENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQ 423
           +NSNGDLVFYLDGAHSPESMEACA+WFSSVVKGNHKS + F AKN+DRDS NDD VKV+Q
Sbjct: 365 DNSNGDLVFYLDGAHSPESMEACASWFSSVVKGNHKSLNSFRAKNVDRDSGNDDPVKVLQ 424

Query: 424 EAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNS 483
           EAES K+SKQ+LLFNCMEVRDP ILLPRLVNTCASSGV+FSKALFVP+MSTY+KV+S NS
Sbjct: 425 EAESTKLSKQVLLFNCMEVRDPHILLPRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNS 484

Query: 484 VSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDN 543
           V+SSD +SKDLSWQ NLQR+WEKIMHGK  DA+ +KDLKLDSER L  LYEDS SSLS+N
Sbjct: 485 VTSSDNISKDLSWQLNLQRLWEKIMHGK--DAILEKDLKLDSERGLPALYEDSLSSLSEN 544

Query: 544 NFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N   SAVMPSLPLAIKWLRDCVRESPS+RLQVLVTGSLH+VGDVLKLL+R
Sbjct: 545 NLSSSAVMPSLPLAIKWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLLKR 558

BLAST of MC00g0258 vs. ExPASy TrEMBL
Match: A0A1S3B8K3 (Folylpolyglutamate synthase OS=Cucumis melo OX=3656 GN=LOC103486953 PE=3 SV=1)

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 465/590 (78.81%), Postives = 504/590 (85.42%), Query Frame = 0

Query: 4   LPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHV 63
           LPQ  SYESAMEALSSLI+QKRRGE V   S +EKL  MRKY+EIL + + V+KLKIIHV
Sbjct: 5   LPQLPSYESAMEALSSLIRQKRRGEMVSAISKEEKLVKMRKYIEILGVGECVKKLKIIHV 64

Query: 64  AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWI 123
           AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 
Sbjct: 65  AGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWT 124

Query: 124 QLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGIT 183
           QLKENVTE+LPMPALFQFLTLLAF IF DE+VDVAIIEVGLGG YDSTNVI+EP+VCGIT
Sbjct: 125 QLKENVTEHLPMPALFQFLTLLAFKIFIDEEVDVAIIEVGLGGTYDSTNVIEEPIVCGIT 184

Query: 184 SLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILK 243
           SLGMDHME LG                                  +SLGMIASHKAGILK
Sbjct: 185 SLGMDHMEILG----------------------------------DSLGMIASHKAGILK 244

Query: 244 PQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAV 303
           PQVPAFTVPQLPEAMDVIQKKAR LM+PLEVAEPLD K+LDGLKLGMSGDHQ VNSGLAV
Sbjct: 245 PQVPAFTVPQLPEAMDVIQKKARELMVPLEVAEPLDVKKLDGLKLGMSGDHQFVNSGLAV 304

Query: 304 SLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTMES 363
           SLCKCWLQRTGNW+R+F NGFK A +P AFLRGL+TANLSGRAQIVYD Y  S +ST +S
Sbjct: 305 SLCKCWLQRTGNWERMFLNGFKGAKIPAAFLRGLSTANLSGRAQIVYDCYTPSFHSTTQS 364

Query: 364 ENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQ 423
           +NSNGDLVFYLDGAHSPESMEACA+WFSSVVKGNHKS +CF AK +DRDS NDD V+V Q
Sbjct: 365 DNSNGDLVFYLDGAHSPESMEACASWFSSVVKGNHKSLNCFRAKTMDRDSGNDDPVEVPQ 424

Query: 424 EAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNS 483
           EAES K+SKQILLFNCMEVRDP ILLPRLVNTCASSGV+FSKALFVP+MSTY+KV+S NS
Sbjct: 425 EAESTKLSKQILLFNCMEVRDPHILLPRLVNTCASSGVHFSKALFVPTMSTYSKVSSGNS 484

Query: 484 VSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDN 543
           V+ SD +SKDLSWQ NLQR+WEKIMHGK  DA+ +KDLKLDSER L  LYEDS SSLS+N
Sbjct: 485 VTPSDNISKDLSWQLNLQRLWEKIMHGK--DAILEKDLKLDSERGLPALYEDSLSSLSEN 544

Query: 544 NFGDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 593
           N   SA +PSLPLAIKWLRDCVRESPS+RLQVLVTGSLH+VGDVLKLL+R
Sbjct: 545 NLSSSAAVPSLPLAIKWLRDCVRESPSIRLQVLVTGSLHLVGDVLKLLKR 558

BLAST of MC00g0258 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 640.6 bits (1651), Expect = 1.3e-183
Identity = 349/596 (58.56%), Postives = 413/596 (69.30%), Query Frame = 0

Query: 3   DLPQSSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIH 62
           D P  SSY+ AMEALS+LI ++ RG++     N +KLE +  Y++ILDLE  +++LK+IH
Sbjct: 76  DNPALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIH 135

Query: 63  VAGTKGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACW 122
           VAGTKGKGSTC F EAILR CG RTG+FTSPHLIDVRERFRI+GLDISE+KFLQYFW CW
Sbjct: 136 VAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECW 195

Query: 123 IQLKENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGI 182
             LKE   + L MP LFQFLT+LAF IF  E+VDVA+IEVGLGG  DSTNVI++PVVCGI
Sbjct: 196 KLLKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGI 255

Query: 183 TSLGMDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGIL 242
            SLGMDHM+ L                                  GN+L  IA HKAGI 
Sbjct: 256 ASLGMDHMDIL----------------------------------GNTLADIAFHKAGIF 315

Query: 243 KPQVPAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLA 302
           KPQ+PAFTVPQL EAMDV+QK A  L +PLEV  PL+ K+LDG+ LG+SGDHQLVN+GLA
Sbjct: 316 KPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKKLDGVTLGLSGDHQLVNAGLA 375

Query: 303 VSLCKCWLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTME 362
           VSL +CWLQRTGNW++IF N  K   +P AF RGLATA L GRAQ+V+D  +   +S+  
Sbjct: 376 VSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARLHGRAQVVHDVVSDPQDSSDS 435

Query: 363 SENSNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVM 422
            E   GDL+FYLDGAHSPESMEAC  WFSS V+G+ KS    +   +       DL    
Sbjct: 436 METPCGDLIFYLDGAHSPESMEACGRWFSSAVRGD-KSLSTAVNGYMRHGEYGTDL---- 495

Query: 423 QEAESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSN 482
                 +VSKQILLFNCMEVRDPQ+LLP+LV TCASSG +FS+ALFVPSMSTY KV S  
Sbjct: 496 -----NRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFSRALFVPSMSTYNKVISGA 555

Query: 483 SVSSSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERML-SHLYEDSHSSLS 542
           S   SD   KDL+WQF LQR+WEK + G   DA  D  LK D    L  H +    +   
Sbjct: 556 SAIPSDTRRKDLTWQFRLQRLWEKSIQG--TDAGLDHTLKPDGITALPPHDFLCGDAPQC 615

Query: 543 DNNFG----DSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 594
               G     SAVMPSLPL I WLRDCVR +PS++L+VLVTGSLH+VGDVL+LL+R
Sbjct: 616 GGPAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLEVLVTGSLHLVGDVLRLLKR 625

BLAST of MC00g0258 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-145
Identity = 293/586 (50.00%), Postives = 378/586 (64.51%), Query Frame = 0

Query: 9   SYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGTKG 68
           SYE A+ ALSSLI ++ R +K   ++  ++ E++  Y+++LDLE+ + K+ +IHVAGTKG
Sbjct: 66  SYEEALAALSSLITKRSRADK---SNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKG 125

Query: 69  KGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLKEN 128
           KGSTCTF E+I+R  G RTGLFTSPHLIDVRERFR++G+DISE+KFL YFW C+ +LKE 
Sbjct: 126 KGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKER 185

Query: 129 VTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLGMD 188
             E +PMP  F+FL LLAF IF+ E+VD AI+EVGLGG +D+TN +++PVVCGI+SLG D
Sbjct: 186 TNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYD 245

Query: 189 HMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQVPA 248
           HME L                                  G++LG IA  KAGI K  VPA
Sbjct: 246 HMEIL----------------------------------GDTLGKIAGEKAGIFKLGVPA 305

Query: 249 FTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLCKC 308
           FTVPQ  EAM V+++KA    + LEV +PL  + L G KLG+ G+HQ VN+GLAVSL   
Sbjct: 306 FTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASI 365

Query: 309 WLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESENSNG 368
           WLQ+ G  +    +  +++ +P+ F++GLATA+L GRAQ+V D+Y  S  S        G
Sbjct: 366 WLQQIGKLE--VPSRTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTS--------G 425

Query: 369 DLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEAESK 428
           DLVFYLDGAHSPESMEACA WFS  VKG+++S       N    S +D          S 
Sbjct: 426 DLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHDKW--------SN 485

Query: 429 KVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVSSSD 488
           +  +QILLFNCM VRDP +LLP L N CA  GVNF KALFVP+MS Y KV ++  +  +D
Sbjct: 486 ETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPEND 545

Query: 489 IMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNFGDS 548
               DLSWQF LQ++WE ++        S++D + D E              SD   G+S
Sbjct: 546 -PQVDLSWQFTLQKVWESLVQ-------SERDGEKDGE--------------SD---GNS 571

Query: 549 AVMPSLPLAIKWLRDCVRESPS-VRLQVLVTGSLHMVGDVLKLLRR 594
            V  SLP+AIK LRD V ES S  R QVLVTGSLH+VGDVL+L+R+
Sbjct: 606 EVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIRK 571

BLAST of MC00g0258 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-145
Identity = 293/586 (50.00%), Postives = 378/586 (64.51%), Query Frame = 0

Query: 9   SYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGTKG 68
           SYE A+ ALSSLI ++ R +K   ++  ++ E++  Y+++LDLE+ + K+ +IHVAGTKG
Sbjct: 8   SYEEALAALSSLITKRSRADK---SNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKG 67

Query: 69  KGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLKEN 128
           KGSTCTF E+I+R  G RTGLFTSPHLIDVRERFR++G+DISE+KFL YFW C+ +LKE 
Sbjct: 68  KGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKER 127

Query: 129 VTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLGMD 188
             E +PMP  F+FL LLAF IF+ E+VD AI+EVGLGG +D+TN +++PVVCGI+SLG D
Sbjct: 128 TNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYD 187

Query: 189 HMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQVPA 248
           HME L                                  G++LG IA  KAGI K  VPA
Sbjct: 188 HMEIL----------------------------------GDTLGKIAGEKAGIFKLGVPA 247

Query: 249 FTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLCKC 308
           FTVPQ  EAM V+++KA    + LEV +PL  + L G KLG+ G+HQ VN+GLAVSL   
Sbjct: 248 FTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASI 307

Query: 309 WLQRTGNWQRIFQNGFKVANMPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESENSNG 368
           WLQ+ G  +    +  +++ +P+ F++GLATA+L GRAQ+V D+Y  S  S        G
Sbjct: 308 WLQQIGKLE--VPSRTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTS--------G 367

Query: 369 DLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEAESK 428
           DLVFYLDGAHSPESMEACA WFS  VKG+++S       N    S +D          S 
Sbjct: 368 DLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHDKW--------SN 427

Query: 429 KVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVSSSD 488
           +  +QILLFNCM VRDP +LLP L N CA  GVNF KALFVP+MS Y KV ++  +  +D
Sbjct: 428 ETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPEND 487

Query: 489 IMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNFGDS 548
               DLSWQF LQ++WE ++        S++D + D E              SD   G+S
Sbjct: 488 -PQVDLSWQFTLQKVWESLVQ-------SERDGEKDGE--------------SD---GNS 513

Query: 549 AVMPSLPLAIKWLRDCVRESPS-VRLQVLVTGSLHMVGDVLKLLRR 594
            V  SLP+AIK LRD V ES S  R QVLVTGSLH+VGDVL+L+R+
Sbjct: 548 EVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIRK 513

BLAST of MC00g0258 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-128
Identity = 266/588 (45.24%), Postives = 356/588 (60.54%), Query Frame = 0

Query: 7   SSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGT 66
           S+ Y++ ++ALSSLI ++ R   +   +   +  ++  Y+++L+LE  V ++KIIHVAGT
Sbjct: 10  SARYQNTLDALSSLITKRGR---LASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGT 69

Query: 67  KGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLK 126
           KGKGSTCTF E+ILR  GLRTGLFTSPHLIDVRERFR+NG++IS++KF+ YFW C+ +LK
Sbjct: 70  KGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLK 129

Query: 127 ENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLG 186
           E  +  +PMP  F FL LLAF IF+ EQVDV I+EVGLGG +D+TNVI++PVVCGI+SLG
Sbjct: 130 EKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLG 189

Query: 187 MDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQV 246
            DHME LG                                   +L  IA+ KAGI K  V
Sbjct: 190 YDHMEILGY----------------------------------TLAEIAAEKAGIFKSGV 249

Query: 247 PAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLC 306
           PAFTV Q  EAM V+ +KA  L + L+V EPLD  +    +LG+ G+HQ +N+GLAV+LC
Sbjct: 250 PAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALC 309

Query: 307 KCWLQRTGNWQRIFQNGFKVAN-MPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESEN 366
             +L+  G   +   NG    N +P+ F+ GL+ A L GRA IV D       S +  E 
Sbjct: 310 STFLKEIGIEDK---NGLDQTNGLPEKFISGLSNAYLMGRAMIVPD-------SELPEE- 369

Query: 367 SNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEA 426
               +V+YLDGAHSPESMEACA WFS  +K N                          + 
Sbjct: 370 ----IVYYLDGAHSPESMEACAIWFSKQIKQN--------------------------QE 429

Query: 427 ESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVS 486
            ++K S+QILLFNCM VRDP +LLPRL + C   GV+F +A+FVP++S Y +V SS +V 
Sbjct: 430 RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVG 489

Query: 487 SSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNF 546
           +     + +SWQF LQRIWE +  G+                  S+   DS     + +F
Sbjct: 490 T---RVESMSWQFGLQRIWESLARGEAK----------------SNSKSDSKGKEEEKSF 492

Query: 547 GDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 594
               V  SLP+A+ WLRD  R+S  VR QVLVTGSLH+VGD+L+ +++
Sbjct: 550 ----VFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 492

BLAST of MC00g0258 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 265/588 (45.07%), Postives = 355/588 (60.37%), Query Frame = 0

Query: 7   SSSYESAMEALSSLIKQKRRGEKVLGTSNDEKLEMMRKYVEILDLEQGVRKLKIIHVAGT 66
           S+ Y++ ++ALSSLI ++ R   +   +   +  ++  Y+++L+LE  V ++KIIHVAGT
Sbjct: 10  SARYQNTLDALSSLITKRGR---LASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGT 69

Query: 67  KGKGSTCTFCEAILRECGLRTGLFTSPHLIDVRERFRINGLDISEDKFLQYFWACWIQLK 126
           KGKGSTCTF E+ILR  GLRTGLFTSPHLIDVRERFR+NG++IS++KF+ YFW C+ +LK
Sbjct: 70  KGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLK 129

Query: 127 ENVTEYLPMPALFQFLTLLAFTIFSDEQVDVAIIEVGLGGMYDSTNVIKEPVVCGITSLG 186
           E  +  +PMP  F FL LLAF IF+ EQVDV I+EVGLGG +D+TN I++PVVCGI+SLG
Sbjct: 130 EKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLG 189

Query: 187 MDHMETLGISFLLLIKFFHLQFFSEFKVGCLNEILTTINVAGNSLGMIASHKAGILKPQV 246
            DHME LG                                   +L  IA+ KAGI K  V
Sbjct: 190 YDHMEILGY----------------------------------TLAEIAAEKAGIFKSGV 249

Query: 247 PAFTVPQLPEAMDVIQKKARALMIPLEVAEPLDHKRLDGLKLGMSGDHQLVNSGLAVSLC 306
           PAFTV Q  EAM V+ +KA  L + L+V EPLD  +    +LG+ G+HQ +N+GLAV+LC
Sbjct: 250 PAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLDSSQ----RLGLQGEHQYLNAGLAVALC 309

Query: 307 KCWLQRTGNWQRIFQNGFKVAN-MPDAFLRGLATANLSGRAQIVYDRYASSHNSTMESEN 366
             +L+  G   +   NG    N +P+ F+ GL+ A L GRA IV D       S +  E 
Sbjct: 310 STFLKEIGIEDK---NGLDQTNGLPEKFISGLSNAYLMGRAMIVPD-------SELPEE- 369

Query: 367 SNGDLVFYLDGAHSPESMEACANWFSSVVKGNHKSPHCFMAKNIDRDSENDDLVKVMQEA 426
               +V+YLDGAHSPESMEACA WFS  +K N                          + 
Sbjct: 370 ----IVYYLDGAHSPESMEACAIWFSKQIKQN--------------------------QE 429

Query: 427 ESKKVSKQILLFNCMEVRDPQILLPRLVNTCASSGVNFSKALFVPSMSTYTKVTSSNSVS 486
            ++K S+QILLFNCM VRDP +LLPRL + C   GV+F +A+FVP++S Y +V SS +V 
Sbjct: 430 RNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVG 489

Query: 487 SSDIMSKDLSWQFNLQRIWEKIMHGKEPDAVSDKDLKLDSERMLSHLYEDSHSSLSDNNF 546
           +     + +SWQF LQRIWE +  G+                  S+   DS     + +F
Sbjct: 490 T---RVESMSWQFGLQRIWESLARGEAK----------------SNSKSDSKGKEEEKSF 491

Query: 547 GDSAVMPSLPLAIKWLRDCVRESPSVRLQVLVTGSLHMVGDVLKLLRR 594
               V  SLP+A+ WLRD  R+S  VR QVLVTGSLH+VGD+L+ +++
Sbjct: 550 ----VFSSLPVAVDWLRDNARQSKQVRFQVLVTGSLHLVGDLLRFIKK 491