Lsi06G005000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G005000
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionbeta-glucosidase 12-like
Locationchr06: 5991708 .. 5993468 (+)
RNA-Seq ExpressionLsi06G005000
SyntenyLsi06G005000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGGTTTGAAAGATAATACCTTCTTTCTTGCCTTTGTTTTGCTTATCCTTATCATAGTTTCAGAGGCCACAGATGAATCAAACTCTCCCATTCCAACTTTACGACGAAGCAATTTCCCTCCAGGCTTTGTTTTCGGTTCTTCCTCTGCTGCTTATCAGGTTAAACTTCTACACATTTTTTTTTTTTTTTTTTCCAATTATTGTCCAATTTTTAAACTAATTAATTAAACTGATGAATATTGTATGAATGACTTATTTGATATTGTTTTTTTGTATATAGTATGAAGGTGCTGCTTTTAAGTATGGGAAAGGCCCAAGTATTTGGGATAACTATACTCATCAACACCCAGATTTTCTTTCTCTCTCTCTTAATAAAAATAAATAATTAAATATAATTTTTCTCTCACTGGAGTTAGAAGAAGTGAGTTGTGTTGTTTCGTTGAAATTGCAGAGAGAATTGCTGATCATAACAATGGAGATTTAGCTGTTGATCAATATCACCGTTATAGGGTTTGTGGAATATATATATATGTGTGTGTGTCCTATATAATAATTTAAGAAAATCATATACATACATAAATATATATTATGCAAATTTGATTGACAACTAGTACTGTTGATATATAATGTAGGAAGATGTTGACATTATGCAACGTATAGGTTTTGATGCTTATAGATTTTCAATTTCCTGGCCTAGGGTCTTACCAAGTAAGAAATTTAAATATCCCCCCTATACTTTAATTTGATCAATTATAAAAGTATTAATATAAGATTTCAACATTTTAAATATGTGTGTATATATATATATACTCAGAGGGAAAACTTAGCGGTGGTGTGAATTTAGAAGGAATTAACTACTACCACAGGCTGATCAATGCACTCCATTCAAAAGGTTGGTATTATCTTAATATTAGATTCTTTAAACCACTTTTTTTTTTTTTTAATATAATTATGTGGGTTTGATTTGAATCAATATTTACAATTCTTAATTTGTAGGCATCAAACCATATGTTACAATTTTCCACTGGGATGTTCCTCAAGTTTTACAAGAAGAATATAAAGGCTTCATGAGTCGTGAAATTTTGTAAGTAACTTTTTCTGAAATTTTCACAGTTTTGGTTATATCAACTAGTAATTTTTTGTCAACAAAATCGCTTCAACCGAAGAATATTAAAAAATAAAAAATAGTTTATATCTTCACAATAAAAATAATAATAATAATAGTGAGAAAATTTATTACAGACATGATTATCGAGACTTCGCTGAACTTTGCTTCAAGGAGTTTGGACATAGAGTGAAACATTGGATCACATTTAATGAACAATACGTCTTCATAACAAATGGTTATGCAACGGGAGCTTTTGCACCAGGCAGATGCTCTTCTTGGCAACAATTGAATTGTCTAGGTGGAGATTCGGGGACTGAACCATACATTGTTGCCCACCACCAAATTCTTGCTCATGCCATTGCTGTTAGACTTTATAAGTCTAAATATCAGGTTTCCTCTCTTAATTGCTATAATAATACATATATAATGTGAAAAAATAAACACAACTTTAAAAAAAAAAAAAAAAAAAGGGTTAATTATTATTTCATGCCTTTTGAACCATTTTTCAATAATATGGGATTTGTAGGCACACCAACAAGGTGAGATTGGAGTCACATTGTATTCAGATTGGTTTGTTCCATATTCAAATAAGGAAGCAGACAAAAGTGCTGCACGTCGAGCTCTTGATTTTTCTCTTGGTTGGTAAGTAA

mRNA sequence

ATGATGGGTTTGAAAGATAATACCTTCTTTCTTGCCTTTGTTTTGCTTATCCTTATCATAGTTTCAGAGGCCACAGATGAATCAAACTCTCCCATTCCAACTTTACGACGAAGCAATTTCCCTCCAGGCTTTGTTTTCGGTTCTTCCTCTGCTGCTTATCAGTATGAAGGTGCTGCTTTTAAGTATGGGAAAGGCCCAAAGAGAATTGCTGATCATAACAATGGAGATTTAGCTGTTGATCAATATCACCGTTATAGGGAAGATGTTGACATTATGCAACGTATAGGTTTTGATGCTTATAGATTTTCAATTTCCTGGCCTAGGGTCTTACCAAAGGGAAAACTTAGCGGTGGTGTGAATTTAGAAGGAATTAACTACTACCACAGGCTGATCAATGCACTCCATTCAAAAGGCATCAAACCATATGTTACAATTTTCCACTGGGATGTTCCTCAAGTTTTACAAGAAGAATATAAAGGCTTCATGAGTCGTGAAATTTTACATGATTATCGAGACTTCGCTGAACTTTGCTTCAAGGAGTTTGGACATAGAGTGAAACATTGGATCACATTTAATGAACAATACGTCTTCATAACAAATGGTTATGCAACGGGAGCTTTTGCACCAGGCAGATGCTCTTCTTGGCAACAATTGAATTGTCTAGGTGGAGATTCGGGGACTGAACCATACATTGTTGCCCACCACCAAATTCTTGCTCATGCCATTGCTGTTAGACTTTATAAGTCTAAATATCAGGTTTCCTCTCTTAATTGCTATAATAATACATATATAATGCACACCAACAAGGTGAGATTGGAGTCACATTGTATTCAGATTGGTTTGTTCCATATTCAAATAAGGAAGCAGACAAAAGTGCTGCACGTCGAGCTCTTGATTTTTCTCTTGGTTGGTAAGTAA

Coding sequence (CDS)

ATGATGGGTTTGAAAGATAATACCTTCTTTCTTGCCTTTGTTTTGCTTATCCTTATCATAGTTTCAGAGGCCACAGATGAATCAAACTCTCCCATTCCAACTTTACGACGAAGCAATTTCCCTCCAGGCTTTGTTTTCGGTTCTTCCTCTGCTGCTTATCAGTATGAAGGTGCTGCTTTTAAGTATGGGAAAGGCCCAAAGAGAATTGCTGATCATAACAATGGAGATTTAGCTGTTGATCAATATCACCGTTATAGGGAAGATGTTGACATTATGCAACGTATAGGTTTTGATGCTTATAGATTTTCAATTTCCTGGCCTAGGGTCTTACCAAAGGGAAAACTTAGCGGTGGTGTGAATTTAGAAGGAATTAACTACTACCACAGGCTGATCAATGCACTCCATTCAAAAGGCATCAAACCATATGTTACAATTTTCCACTGGGATGTTCCTCAAGTTTTACAAGAAGAATATAAAGGCTTCATGAGTCGTGAAATTTTACATGATTATCGAGACTTCGCTGAACTTTGCTTCAAGGAGTTTGGACATAGAGTGAAACATTGGATCACATTTAATGAACAATACGTCTTCATAACAAATGGTTATGCAACGGGAGCTTTTGCACCAGGCAGATGCTCTTCTTGGCAACAATTGAATTGTCTAGGTGGAGATTCGGGGACTGAACCATACATTGTTGCCCACCACCAAATTCTTGCTCATGCCATTGCTGTTAGACTTTATAAGTCTAAATATCAGGTTTCCTCTCTTAATTGCTATAATAATACATATATAATGCACACCAACAAGGTGAGATTGGAGTCACATTGTATTCAGATTGGTTTGTTCCATATTCAAATAAGGAAGCAGACAAAAGTGCTGCACGTCGAGCTCTTGATTTTTCTCTTGGTTGGTAAGTAA

Protein sequence

MMGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKGPKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIAVRLYKSKYQVSSLNCYNNTYIMHTNKVRLESHCIQIGLFHIQIRKQTKVLHVELLIFLLVGK
Homology
BLAST of Lsi06G005000 vs. ExPASy Swiss-Prot
Match: P26205 (Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.0e-89
Identity = 151/243 (62.14%), Postives = 185/243 (76.13%), Query Frame = 0

Query: 35  LRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG-----------PKRIADHNNGDLAVDQYH 94
           L RS F PGFVFG++S+A+QYEGAAF+ GKG           P++I D  NGD+A+D+YH
Sbjct: 25  LNRSCFAPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHKYPEKIKDRTNGDVAIDEYH 84

Query: 95  RYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEGINYYHRLINALHSKGIKPYV 154
           RY+ED+ IM+ +  DAYRFSISWPRVLPKGKLSGGVN EGINYY+ LIN + + G++PYV
Sbjct: 85  RYKEDIGIMKDMNLDAYRFSISWPRVLPKGKLSGGVNREGINYYNNLINEVLANGMQPYV 144

Query: 155 TIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGHRVKHWITFNEQYVFITNGYA 214
           T+FHWDVPQ L++EY+GF+ R I+ D+RD+AELCFKEFG RVKHWIT NE +    N YA
Sbjct: 145 TLFHWDVPQALEDEYRGFLGRNIVDDFRDYAELCFKEFGDRVKHWITLNEPWGVSMNAYA 204

Query: 215 TGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIAVRLYKSKYQVSSLNCYNNTY 267
            G FAPGRCS W +LNC GGDSG EPY+ AH+Q+LAHA A RLYK+KYQ S       T 
Sbjct: 205 YGTFAPGRCSDWLKLNCTGGDSGREPYLAAHYQLLAHAAAARLYKTKYQASQNGIIGITL 264

BLAST of Lsi06G005000 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.5e-84
Identity = 145/263 (55.13%), Postives = 190/263 (72.24%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           LL+ ++ S A + +  P P  RRS FP GF+FG++S++YQYEG A + G+G         
Sbjct: 16  LLLAVVASGAYNSAGEP-PVSRRS-FPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTH 75

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P++IAD +NGD+A D YH Y+EDV +M+ +G DAYRFSISW R+LP G L GGVN EG
Sbjct: 76  QHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEG 135

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN L SKG++P++T+FHWD PQ L+++Y GF+S  I++D++D+AE+CFKEFG 
Sbjct: 136 IKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGD 195

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 254
           RVK+WITFNE + F +NGYATG FAPGRCS W++ NC  GDSG EPY   HHQ+LAHA  
Sbjct: 196 RVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAET 255

Query: 255 VRLYKSKYQVSSLNCYNNTYIMH 267
           VRLYK+KYQ         T + H
Sbjct: 256 VRLYKAKYQALQKGKIGITLVSH 276

BLAST of Lsi06G005000 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-83
Identity = 145/263 (55.13%), Postives = 190/263 (72.24%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           LL+ ++ S A + +  P P  RRS FP GF+FG++S++YQYEG A + G+G         
Sbjct: 16  LLLAVVASGAYNGAGEP-PVSRRS-FPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTH 75

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P++IAD +NGD+A D YH Y+EDV +M+ +G DAYRFSISW R+LP G L GGVN EG
Sbjct: 76  QHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEG 135

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN L SKG++P++T+FHWD PQ L+++Y GF+S  I++D++D+AE+CFKEFG 
Sbjct: 136 IKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGD 195

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 254
           RVK+WITFNE + F +NGYATG FAPGRCS W++ NC  GDSG EPY   HHQ+LAHA  
Sbjct: 196 RVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAET 255

Query: 255 VRLYKSKYQVSSLNCYNNTYIMH 267
           VRLYK+KYQ         T + H
Sbjct: 256 VRLYKAKYQALQKGKIGITLVSH 276

BLAST of Lsi06G005000 vs. ExPASy Swiss-Prot
Match: Q7F9K4 (Beta-glucosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU10 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 4.8e-83
Identity = 148/287 (51.57%), Postives = 195/287 (67.94%), Query Frame = 0

Query: 14  VLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKGPK------ 73
           VLL+L+  + A       +P + R +FP GF+FG+SS++YQ+EGAA K G+GP       
Sbjct: 13  VLLLLLAFTCAAYNDAGELPPISRRSFPKGFIFGTSSSSYQFEGAAAKGGRGPSIWDTFT 72

Query: 74  -----RIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLE 133
                +I D +NGD A + YH Y+EDV IM+ +G DAYRFSISW R+LP G LSGGVN E
Sbjct: 73  HQYPDKITDKSNGDGACNSYHLYKEDVRIMKEMGMDAYRFSISWSRILPNGSLSGGVNRE 132

Query: 134 GINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFG 193
           GINYY+ LIN L SK ++P+ T+FH+D PQ L+++YKGF+S  I++DY+D+AE+CFKEFG
Sbjct: 133 GINYYNNLINELLSKEVQPFATLFHFDTPQALEDKYKGFLSPNIINDYKDYAEICFKEFG 192

Query: 194 HRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAI 253
            RVKHWITFNE + F + GYA+G  APGRCSSW++  C  GDSG EPY   HHQ+LAHA 
Sbjct: 193 DRVKHWITFNEPWNFCSMGYASGTMAPGRCSSWEKGKCRVGDSGREPYTACHHQLLAHAE 252

Query: 254 AVRLYKSKYQVSSLNCYNNTYIMHTNKVRLESHCIQIGLFHIQIRKQ 290
            VRLYK KYQ +      + +I   N  R  +  +Q G   I +  +
Sbjct: 253 TVRLYKEKYQFTEEAIRQSPFIRDNNLNRRSAKALQKGRIGIILNSE 299

BLAST of Lsi06G005000 vs. ExPASy Swiss-Prot
Match: B7ECS8 (Putative beta-glucosidase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU9 PE=5 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-80
Identity = 140/275 (50.91%), Postives = 193/275 (70.18%), Query Frame = 0

Query: 11  LAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKGPK--- 70
           L  +LL+L++++ A  E+   +P + R +FP GF+FG+SSA+YQ EG A + G+GP    
Sbjct: 14  LLLLLLLLLLLAAACVEAGE-LPPISRRSFPKGFIFGTSSASYQCEGGAAEGGRGPSIWD 73

Query: 71  --------RIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGV 130
                   +IAD +NGD+A + YH Y+EDV +M+ +G DAYRFSISW R+LP G L+GGV
Sbjct: 74  TFTYQHPDKIADKSNGDVADNTYHLYKEDVHMMKEMGMDAYRFSISWSRILPNGSLNGGV 133

Query: 131 NLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFK 190
           N+EGINYY+ LIN L  KG++ +VT+FH+D PQ L+++Y GF+S  I++DY+D+AE+CFK
Sbjct: 134 NIEGINYYNNLINELLLKGVQSFVTLFHYDTPQALEDKYNGFLSPNIINDYKDYAEICFK 193

Query: 191 EFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILA 250
           EFG RVKHWITFNE ++F +  YA+G +APGRCS W+   C  GDSG EPY   HHQ+LA
Sbjct: 194 EFGDRVKHWITFNEPWIFCSKAYASGTYAPGRCSPWEMGKCSVGDSGREPYTACHHQLLA 253

Query: 251 HAIAVRLYKSKYQVSSLNCYNNTYIMHTNKVRLES 275
           HA  VRLY+ KYQ +       +  +H N +   S
Sbjct: 254 HAETVRLYREKYQFTE-EVVRQSQFIHDNDLHRRS 286

BLAST of Lsi06G005000 vs. ExPASy TrEMBL
Match: A0A0A0LXF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611300 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 4.0e-104
Identity = 185/272 (68.01%), Postives = 222/272 (81.62%), Query Frame = 0

Query: 2   MGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFK 61
           MG K +TFFL  V LI +IVSEA  + +SPIP +R+SNFP  FVFGSSS+AYQYEGA   
Sbjct: 1   MGFK-HTFFLGLVFLISLIVSEAARQPSSPIPIIRKSNFPKDFVFGSSSSAYQYEGAVDI 60

Query: 62  YGKGPKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNL 121
            G+ PKRIAD  NGD+AVD+YHRY+EDV IM+RIGF AYRFSISW R+LPKGKL GGVN 
Sbjct: 61  DGRKPKRIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSRILPKGKLIGGVNK 120

Query: 122 EGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEF 181
           +GI+YY+RLIN L SKGI+ YVTIFHWDVPQ L++ Y+GF+S +I++DY+DFAELCFKEF
Sbjct: 121 KGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIINDYQDFAELCFKEF 180

Query: 182 GHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHA 241
           G RVKHWITFNEQYVFI NGY  GAFAPGRCSSWQ  NCLGG+SGTEPYIV H+QIL+HA
Sbjct: 181 GDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCLGGNSGTEPYIVGHYQILSHA 240

Query: 242 IAVRLYKSKYQVS-----SLNCYNNTYIMHTN 269
            AV++YKSKYQ        +  ++N ++ ++N
Sbjct: 241 AAVKIYKSKYQAHQKGEIGVTLFSNWFVPYSN 271

BLAST of Lsi06G005000 vs. ExPASy TrEMBL
Match: A0A6J1JG74 (beta-glucosidase 12-like OS=Cucurbita maxima OX=3661 GN=LOC111485433 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 8.2e-102
Identity = 174/270 (64.44%), Postives = 217/270 (80.37%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           +L++++VS+A  E + PI  +RRS+FP GFVFGSSS+AYQYEGAAFKYGKG         
Sbjct: 15  VLVIVVVSKAAVEPSHPIDAVRRSSFPKGFVFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 74

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P+RIAD +NGD+A D YHRY+ED+ IM++IGFDA+RFSISWPRV PKGKLSGGVN+EG
Sbjct: 75  QHPERIADGSNGDIAADSYHRYKEDIAIMKQIGFDAFRFSISWPRVFPKGKLSGGVNMEG 134

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN L + GIKPYVT+FHWD+PQ LQ+EY+GFM   +++D+RD+AELCFKEFG 
Sbjct: 135 IKYYNNLINELLAHGIKPYVTLFHWDLPQALQDEYQGFMDHRVINDFRDYAELCFKEFGD 194

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 254
           RVKHWITFNEQY+FITNGY  G FAPGRCSSWQ  NCLGGDS TEPYIV H+QILAHA A
Sbjct: 195 RVKHWITFNEQYIFITNGYVYGQFAPGRCSSWQPFNCLGGDSATEPYIVGHNQILAHAAA 254

Query: 255 VRLYKSKYQVS-----SLNCYNNTYIMHTN 269
           V++YK+KY+        +  ++N Y+ +++
Sbjct: 255 VKVYKTKYKAHQRGEIGVTLFSNWYVPYSD 284

BLAST of Lsi06G005000 vs. ExPASy TrEMBL
Match: A0A5D3DW45 (Beta-glucosidase 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold93G00040 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 3.1e-101
Identity = 183/284 (64.44%), Postives = 221/284 (77.82%), Query Frame = 0

Query: 1   MMGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAF 60
           MMG + +TFFL  V LI +IVSEA+ + +SPIP +RRSNFP  FVFGSSS+AYQYEGA  
Sbjct: 1   MMGFR-HTFFLGLVFLISLIVSEASHQPSSPIPIVRRSNFPKNFVFGSSSSAYQYEGAVA 60

Query: 61  KYG-----------KGPKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRV 120
             G           K P+RIAD  NGD+AVD+YHRY+EDV IM+RIGF AYRFSISW R+
Sbjct: 61  TDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSRI 120

Query: 121 LPKGKLSGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHD 180
           +P GKL  GVN +GI+YY+RLIN L SKGI+ YVTIFHWDVPQ L++ Y+GF+S +I++D
Sbjct: 121 IPTGKLKDGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIND 180

Query: 181 YRDFAELCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEP 240
           Y DFAELCFKEFG RVKHWITFNEQYVFI NGY  GAFAPGRCSSWQ  NC GG+SGTEP
Sbjct: 181 YGDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCTGGNSGTEP 240

Query: 241 YIVAHHQILAHAIAVRLYKSKYQVS-----SLNCYNNTYIMHTN 269
           YIV HHQIL+HA+AV++YKSKYQ        +  ++N ++ ++N
Sbjct: 241 YIVGHHQILSHAVAVKIYKSKYQARQKGEIGVTLFSNWFVPYSN 283

BLAST of Lsi06G005000 vs. ExPASy TrEMBL
Match: A0A6J1H9N6 (beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461393 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 3.1e-101
Identity = 169/249 (67.87%), Postives = 208/249 (83.53%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           +L++++VS+A  E + PI  +RRS+FP GF+FGSSS+AYQYEGAAFKYGKG         
Sbjct: 15  MLVIVVVSKAAVEPSHPIDAVRRSSFPKGFIFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 74

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P+RIAD +NGD+A + YHRY+EDV I++RIGFDAYRFSISWPRVLP+GKLSGGVN EG
Sbjct: 75  QHPERIADRSNGDIATNSYHRYKEDVAIIKRIGFDAYRFSISWPRVLPQGKLSGGVNKEG 134

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN LH+ GIKPYVT+FHWD+PQ LQ+EY+GF+S  ++ D+RD+ ++CFKEFG 
Sbjct: 135 IEYYNNLINELHAHGIKPYVTLFHWDLPQALQDEYQGFLSHRVISDFRDYVDICFKEFGD 194

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 253
           RVKHWIT NEQY+FIT+GY  G FAPGRCSSWQ  NCLGGDSGTEPYIV H+QIL+HA A
Sbjct: 195 RVKHWITLNEQYIFITSGYVFGKFAPGRCSSWQPFNCLGGDSGTEPYIVGHNQILSHATA 254

BLAST of Lsi06G005000 vs. ExPASy TrEMBL
Match: A0A6J1H828 (beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461393 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 3.1e-101
Identity = 169/249 (67.87%), Postives = 208/249 (83.53%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           +L++++VS+A  E + PI  +RRS+FP GF+FGSSS+AYQYEGAAFKYGKG         
Sbjct: 15  MLVIVVVSKAAVEPSHPIDAVRRSSFPKGFIFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 74

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P+RIAD +NGD+A + YHRY+EDV I++RIGFDAYRFSISWPRVLP+GKLSGGVN EG
Sbjct: 75  QHPERIADRSNGDIATNSYHRYKEDVAIIKRIGFDAYRFSISWPRVLPQGKLSGGVNKEG 134

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN LH+ GIKPYVT+FHWD+PQ LQ+EY+GF+S  ++ D+RD+ ++CFKEFG 
Sbjct: 135 IEYYNNLINELHAHGIKPYVTLFHWDLPQALQDEYQGFLSHRVISDFRDYVDICFKEFGD 194

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 253
           RVKHWIT NEQY+FIT+GY  G FAPGRCSSWQ  NCLGGDSGTEPYIV H+QIL+HA A
Sbjct: 195 RVKHWITLNEQYIFITSGYVFGKFAPGRCSSWQPFNCLGGDSGTEPYIVGHNQILSHATA 254

BLAST of Lsi06G005000 vs. NCBI nr
Match: XP_038879813.1 (beta-glucosidase 12-like [Benincasa hispida])

HSP 1 Score: 411.0 bits (1055), Expect = 9.0e-111
Identity = 200/263 (76.05%), Postives = 223/263 (84.79%), Query Frame = 0

Query: 1   MMGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAF 60
           M+G KD TFFL F+ LIL +VS+A  + +SPIP +RRSNFP  FVFGSSSAAYQYEGAAF
Sbjct: 1   MLGFKD-TFFLGFLSLIL-LVSDAAHQPSSPIPIIRRSNFPKDFVFGSSSAAYQYEGAAF 60

Query: 61  KYG-----------KGPKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRV 120
           +YG           K P+RIAD  NGD+AVDQYHRY+EDV IM RIGFDAYRFSISW RV
Sbjct: 61  QYGRKPSIWDTYTHKHPERIADGKNGDIAVDQYHRYKEDVAIMDRIGFDAYRFSISWSRV 120

Query: 121 LPKGKLSGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHD 180
           LPKGKLSGGVN EGI+YY+RLIN L SKGI+ YVTIFHWDVPQ LQ+EY+GFM+  I++D
Sbjct: 121 LPKGKLSGGVNKEGIDYYNRLINELLSKGIQSYVTIFHWDVPQALQDEYQGFMNHSIIND 180

Query: 181 YRDFAELCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEP 240
           YRDFAELCFKEFG RVKHWITFNEQYVFI NGYATG+FAPGRCSSWQ  NCLGGDSGTEP
Sbjct: 181 YRDFAELCFKEFGDRVKHWITFNEQYVFIINGYATGSFAPGRCSSWQPFNCLGGDSGTEP 240

Query: 241 YIVAHHQILAHAIAVRLYKSKYQ 253
           YIV HHQIL+HA+AV+LYKSKYQ
Sbjct: 241 YIVGHHQILSHAVAVKLYKSKYQ 261

BLAST of Lsi06G005000 vs. NCBI nr
Match: XP_038879838.1 (beta-glucosidase 12-like [Benincasa hispida])

HSP 1 Score: 408.3 bits (1048), Expect = 5.8e-110
Identity = 202/262 (77.10%), Postives = 219/262 (83.59%), Query Frame = 0

Query: 2   MGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFK 61
           M  KD TFFL F+ LIL + SEAT+E  +PIP +RRSNFP  FVFGSSSAAYQYEGAAFK
Sbjct: 1   MAFKD-TFFLGFLFLIL-VASEATNEPKTPIPIVRRSNFPKDFVFGSSSAAYQYEGAAFK 60

Query: 62  YGKG-----------PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVL 121
            G+G           P+RIAD  NGDLAVDQYHRY+EDV IM RIGFDAYRFSISW RVL
Sbjct: 61  DGRGPSIWDTYTHQHPERIADGKNGDLAVDQYHRYKEDVAIMDRIGFDAYRFSISWSRVL 120

Query: 122 PKGKLSGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDY 181
           PKGKLSGGVN +GI+YY++LIN L SKGI+ YVTIFHWDVPQ LQ+EY+GFMSR+IL DY
Sbjct: 121 PKGKLSGGVNKKGIDYYNKLINELLSKGIQSYVTIFHWDVPQALQDEYQGFMSRKILKDY 180

Query: 182 RDFAELCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPY 241
           RDFAELCFKEFG RVKHWITFNEQYVF  NGYA G FAPGRCSSWQ LNCLGGDSGTEPY
Sbjct: 181 RDFAELCFKEFGDRVKHWITFNEQYVFTINGYAIGVFAPGRCSSWQPLNCLGGDSGTEPY 240

Query: 242 IVAHHQILAHAIAVRLYKSKYQ 253
           IVAHH ILAHA+A RLYKSKYQ
Sbjct: 241 IVAHHLILAHALAARLYKSKYQ 260

BLAST of Lsi06G005000 vs. NCBI nr
Match: XP_023516696.1 (beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.9 bits (982), Expect = 2.6e-102
Identity = 178/270 (65.93%), Postives = 216/270 (80.00%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           +L+ ++VS+A  E + PI  +RRS+FP GFVFGSSS+AYQYEGAAFKYGKG         
Sbjct: 15  MLVTLVVSKAAVEPSHPIDAVRRSSFPKGFVFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 74

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P+RIAD +NGD+A D YHRY+EDV IM+RIGFDAYRFSISWPR+LPKGKLSGGVN +G
Sbjct: 75  QHPERIADRSNGDIAADSYHRYKEDVAIMKRIGFDAYRFSISWPRLLPKGKLSGGVNTQG 134

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YYH LIN L + GIKPYVT+FHWD+PQ LQ+EY+GFM   I++D+RD+AELCFKEFG+
Sbjct: 135 IVYYHNLINELLAHGIKPYVTLFHWDLPQALQDEYQGFMDDRIINDFRDYAELCFKEFGN 194

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 254
           +VKHWITFNEQY+FITNGY  G FAPGRCSSWQ  NCLGGDS TEPYIV H+QILAHA A
Sbjct: 195 KVKHWITFNEQYIFITNGYVFGQFAPGRCSSWQPFNCLGGDSATEPYIVGHNQILAHAAA 254

Query: 255 VRLYKSKYQVS-----SLNCYNNTYIMHTN 269
           V +YK+KYQ        +  ++N Y+ +++
Sbjct: 255 VNVYKTKYQAHQRGEIGVTLFSNWYVPYSD 284

BLAST of Lsi06G005000 vs. NCBI nr
Match: XP_011660113.1 (beta-glucosidase 12 [Cucumis sativus] >KGN66763.2 hypothetical protein Csa_007267 [Cucumis sativus])

HSP 1 Score: 380.6 bits (976), Expect = 1.3e-101
Identity = 186/284 (65.49%), Postives = 223/284 (78.52%), Query Frame = 0

Query: 1   MMGLKDNTFFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAF 60
           MMG K +TFFL  V LI +IVSEA  + +SPIP +R+SNFP  FVFGSSS+AYQYEGA  
Sbjct: 1   MMGFK-HTFFLGLVFLISLIVSEAARQPSSPIPIIRKSNFPKDFVFGSSSSAYQYEGAVD 60

Query: 61  KYG-----------KGPKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRV 120
             G           K P+RIAD  NGD+AVD+YHRY+EDV IM+RIGF AYRFSISW R+
Sbjct: 61  IDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSRI 120

Query: 121 LPKGKLSGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHD 180
           LPKGKL GGVN +GI+YY+RLIN L SKGI+ YVTIFHWDVPQ L++ Y+GF+S +I++D
Sbjct: 121 LPKGKLIGGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIND 180

Query: 181 YRDFAELCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEP 240
           Y+DFAELCFKEFG RVKHWITFNEQYVFI NGY  GAFAPGRCSSWQ  NCLGG+SGTEP
Sbjct: 181 YQDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCLGGNSGTEP 240

Query: 241 YIVAHHQILAHAIAVRLYKSKYQVS-----SLNCYNNTYIMHTN 269
           YIV H+QIL+HA AV++YKSKYQ        +  ++N ++ ++N
Sbjct: 241 YIVGHYQILSHAAAVKIYKSKYQAHQKGEIGVTLFSNWFVPYSN 283

BLAST of Lsi06G005000 vs. NCBI nr
Match: XP_022988081.1 (beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 380.2 bits (975), Expect = 1.7e-101
Identity = 174/270 (64.44%), Postives = 217/270 (80.37%), Query Frame = 0

Query: 15  LLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--------- 74
           +L++++VS+A  E + PI  +RRS+FP GFVFGSSS+AYQYEGAAFKYGKG         
Sbjct: 15  VLVIVVVSKAAVEPSHPIDAVRRSSFPKGFVFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 74

Query: 75  --PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLEG 134
             P+RIAD +NGD+A D YHRY+ED+ IM++IGFDA+RFSISWPRV PKGKLSGGVN+EG
Sbjct: 75  QHPERIADGSNGDIAADSYHRYKEDIAIMKQIGFDAFRFSISWPRVFPKGKLSGGVNMEG 134

Query: 135 INYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFGH 194
           I YY+ LIN L + GIKPYVT+FHWD+PQ LQ+EY+GFM   +++D+RD+AELCFKEFG 
Sbjct: 135 IKYYNNLINELLAHGIKPYVTLFHWDLPQALQDEYQGFMDHRVINDFRDYAELCFKEFGD 194

Query: 195 RVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAIA 254
           RVKHWITFNEQY+FITNGY  G FAPGRCSSWQ  NCLGGDS TEPYIV H+QILAHA A
Sbjct: 195 RVKHWITFNEQYIFITNGYVYGQFAPGRCSSWQPFNCLGGDSATEPYIVGHNQILAHAAA 254

Query: 255 VRLYKSKYQVS-----SLNCYNNTYIMHTN 269
           V++YK+KY+        +  ++N Y+ +++
Sbjct: 255 VKVYKTKYKAHQRGEIGVTLFSNWYVPYSD 284

BLAST of Lsi06G005000 vs. TAIR 10
Match: AT3G60130.1 (beta glucosidase 16 )

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-77
Identity = 131/256 (51.17%), Postives = 183/256 (71.48%), Query Frame = 0

Query: 10  FLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG---- 69
           FL+ +LLI +     + + +S  P LRR++FP  FVFGS+++AYQ EGAA + G+G    
Sbjct: 5   FLSLLLLITLACIGVSAKKHSTRPRLRRNDFPQDFVFGSATSAYQCEGAAHEDGRGPSIW 64

Query: 70  -------PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGG 129
                  P++I D +NG +A D Y+ Y+EDV+++ +IGFDAYRFSISW R+LP+G L GG
Sbjct: 65  DSFSEKFPEKIMDGSNGSIADDSYNLYKEDVNLLHQIGFDAYRFSISWSRILPRGTLKGG 124

Query: 130 VNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCF 189
           +N  GI YY+ LIN L SKG+KP+VT+FHWD+P  L+  Y G +  E ++D+RD+AELCF
Sbjct: 125 INQAGIEYYNNLINQLISKGVKPFVTLFHWDLPDALENAYGGLLGDEFVNDFRDYAELCF 184

Query: 190 KEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQIL 249
           ++FG RVK W T NE Y  +  GY TG  APGRCS++ + +CLGGD+ TEPYIV H+ +L
Sbjct: 185 QKFGDRVKQWTTLNEPYTMVHEGYITGQKAPGRCSNFYKPDCLGGDAATEPYIVGHNLLL 244

Query: 250 AHAIAVRLYKSKYQVS 255
           AH +AV++Y+ KYQ +
Sbjct: 245 AHGVAVKVYREKYQAT 260

BLAST of Lsi06G005000 vs. TAIR 10
Match: AT5G42260.1 (beta glucosidase 12 )

HSP 1 Score: 286.2 bits (731), Expect = 3.1e-77
Identity = 130/259 (50.19%), Postives = 181/259 (69.88%), Query Frame = 0

Query: 8   TFFLAFVLLILII-VSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG- 67
           T +L+ ++ I+++ ++E   + +S  P LRRS+FP  F+FG++++AYQ EGAA + G+G 
Sbjct: 3   TIYLSLLVFIIVLALNEVMAKKHSSTPKLRRSDFPEDFIFGAATSAYQVEGAAHEDGRGP 62

Query: 68  ----------PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKL 127
                     P++I D +NG +A D YH Y+EDV ++ +IGFDAYRFSISW R+LP+  L
Sbjct: 63  SIWDTFSEKYPEKIKDGSNGSIASDSYHLYKEDVGLLHQIGFDAYRFSISWSRILPRENL 122

Query: 128 SGGVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAE 187
            GG+N  GI+YY+ LIN L SKGIKP+ TIFHWD PQ L++ Y GF+  EI++D+RD+A+
Sbjct: 123 KGGINQAGIDYYNNLINELLSKGIKPFATIFHWDTPQSLEDAYGGFLGAEIVNDFRDYAD 182

Query: 188 LCFKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHH 247
           +CFK FG RVKHW+T NE    +  GY  G  APGRCS +   NC  G+  TEPYIV H+
Sbjct: 183 ICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHN 242

Query: 248 QILAHAIAVRLYKSKYQVS 255
            ILAH  AV++Y+ KY+ S
Sbjct: 243 LILAHGEAVKVYREKYKAS 261

BLAST of Lsi06G005000 vs. TAIR 10
Match: AT5G44640.1 (beta glucosidase 13 )

HSP 1 Score: 285.8 bits (730), Expect = 4.1e-77
Identity = 130/257 (50.58%), Postives = 175/257 (68.09%), Query Frame = 0

Query: 9   FFLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG--- 68
           +F   V +I++  +E   + +S  P LRRS+FP  F+FG++++AYQ EGAA + G+G   
Sbjct: 5   YFSLLVFIIVLASNEVIAKKHSSTPKLRRSDFPKDFIFGAATSAYQVEGAAHEDGRGPSI 64

Query: 69  --------PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSG 128
                   P++I D  NG +A D YH Y+EDV ++ +IGF AYRFSISW R+LP+G L G
Sbjct: 65  WDTFSEKYPEKIKDGTNGSIASDSYHLYKEDVGLLHQIGFGAYRFSISWSRILPRGNLKG 124

Query: 129 GVNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELC 188
           G+N  GI+YY+ LIN L SKGIKP+ TIFHWD PQ L++ Y GF   EI++D+RD+A++C
Sbjct: 125 GINQAGIDYYNNLINELLSKGIKPFATIFHWDTPQSLEDAYGGFFGAEIVNDFRDYADIC 184

Query: 189 FKEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQI 248
           FK FG RVKHW+T NE    +  GY  G  APGRCS +   NC  G+  TEPYIV H+ I
Sbjct: 185 FKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLI 244

Query: 249 LAHAIAVRLYKSKYQVS 255
           LAH  AV++Y+ KY+ S
Sbjct: 245 LAHGEAVKVYREKYKAS 261

BLAST of Lsi06G005000 vs. TAIR 10
Match: AT2G44450.1 (beta glucosidase 15 )

HSP 1 Score: 282.0 bits (720), Expect = 5.9e-76
Identity = 127/252 (50.40%), Postives = 175/252 (69.44%), Query Frame = 0

Query: 14  VLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG-------- 73
           V+LI++  ++    +NS  P LRRS+FP  F+FGS+++AYQ EG A + G+G        
Sbjct: 10  VVLIVLASNDVLANNNSSTPKLRRSDFPEDFIFGSATSAYQVEGGAHEDGRGPSIWDTFS 69

Query: 74  ---PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGGVNLE 133
              P++I D +NG +A + YH Y+EDV ++ +IGF+AYRFSISW R+LP+G L GG+N  
Sbjct: 70  EKYPEKIKDGSNGSVADNSYHLYKEDVALLHQIGFNAYRFSISWSRILPRGNLKGGINQA 129

Query: 134 GINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCFKEFG 193
           GI+YY+ LIN L SKGIKP+ T+FHWD PQ L++ Y GF   EI++D+RD+A++CFK FG
Sbjct: 130 GIDYYNNLINELLSKGIKPFATMFHWDTPQALEDAYGGFRGAEIVNDFRDYADICFKNFG 189

Query: 194 HRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQILAHAI 253
            RVKHW+T NE    +  GY  G  APGRCS +   NC  G+  TEPYIV H+ IL+H  
Sbjct: 190 DRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTDGNGATEPYIVGHNLILSHGA 249

Query: 254 AVRLYKSKYQVS 255
           AV++Y+ KY+ S
Sbjct: 250 AVQVYREKYKAS 261

BLAST of Lsi06G005000 vs. TAIR 10
Match: AT2G25630.1 (beta glucosidase 14 )

HSP 1 Score: 281.6 bits (719), Expect = 7.7e-76
Identity = 126/256 (49.22%), Postives = 177/256 (69.14%), Query Frame = 0

Query: 10  FLAFVLLILIIVSEATDESNSPIPTLRRSNFPPGFVFGSSSAAYQYEGAAFKYGKG---- 69
           + + ++ I++  +E   + +S  P LR+++FP  F+FG++++AYQ EGAA + G+G    
Sbjct: 5   YFSVLVFIILASNEVVAKRHSSTPKLRKTDFPEDFIFGAATSAYQVEGAAQEDGRGPSIW 64

Query: 70  -------PKRIADHNNGDLAVDQYHRYREDVDIMQRIGFDAYRFSISWPRVLPKGKLSGG 129
                  P++I D +NG +A D YH Y+EDV ++ +IGF+AYRFSISW R+LP+G L GG
Sbjct: 65  DTFSEKYPEKIKDGSNGSIADDSYHLYKEDVGLLHQIGFNAYRFSISWSRILPRGNLKGG 124

Query: 130 VNLEGINYYHRLINALHSKGIKPYVTIFHWDVPQVLQEEYKGFMSREILHDYRDFAELCF 189
           +N  GI+YY+ LIN L SKGIKP+ TIFHWD PQ L++ Y GF   EI++D+RD+A++CF
Sbjct: 125 INQAGIDYYNNLINELLSKGIKPFATIFHWDTPQDLEDAYGGFRGAEIVNDFRDYADICF 184

Query: 190 KEFGHRVKHWITFNEQYVFITNGYATGAFAPGRCSSWQQLNCLGGDSGTEPYIVAHHQIL 249
           K FG RVKHWIT NE    +  GY  G  APGRCS +   NC  G+  TEPYIV H+ IL
Sbjct: 185 KSFGDRVKHWITLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLIL 244

Query: 250 AHAIAVRLYKSKYQVS 255
           AH  A+++Y+ KY+ S
Sbjct: 245 AHGEAIKVYRKKYKAS 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P262052.0e-8962.14Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV... [more]
B8AVF07.5e-8455.13Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1[more]
Q7XKV41.3e-8355.13Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2[more]
Q7F9K44.8e-8351.57Beta-glucosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU10 PE=2 SV=1[more]
B7ECS81.0e-8050.91Putative beta-glucosidase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU9 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0LXF34.0e-10468.01Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611300 PE=3 SV=1[more]
A0A6J1JG748.2e-10264.44beta-glucosidase 12-like OS=Cucurbita maxima OX=3661 GN=LOC111485433 PE=3 SV=1[more]
A0A5D3DW453.1e-10164.44Beta-glucosidase 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1H9N63.1e-10167.87beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11146139... [more]
A0A6J1H8283.1e-10167.87beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146139... [more]
Match NameE-valueIdentityDescription
XP_038879813.19.0e-11176.05beta-glucosidase 12-like [Benincasa hispida][more]
XP_038879838.15.8e-11077.10beta-glucosidase 12-like [Benincasa hispida][more]
XP_023516696.12.6e-10265.93beta-glucosidase 12-like [Cucurbita pepo subsp. pepo][more]
XP_011660113.11.3e-10165.49beta-glucosidase 12 [Cucumis sativus] >KGN66763.2 hypothetical protein Csa_00726... [more]
XP_022988081.11.7e-10164.44beta-glucosidase 12-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G60130.11.1e-7751.17beta glucosidase 16 [more]
AT5G42260.13.1e-7750.19beta glucosidase 12 [more]
AT5G44640.14.1e-7750.58beta glucosidase 13 [more]
AT2G44450.15.9e-7650.40beta glucosidase 15 [more]
AT2G25630.17.7e-7649.22beta glucosidase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001360Glycoside hydrolase family 1PFAMPF00232Glyco_hydro_1coord: 38..258
e-value: 7.6E-78
score: 261.9
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 28..253
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 19..275
e-value: 1.0E-99
score: 336.1
NoneNo IPR availablePANTHERPTHR10353:SF272SUBFAMILY NOT NAMEDcoord: 28..253
IPR033132Glycosyl hydrolases family 1, N-terminal conserved sitePROSITEPS00653GLYCOSYL_HYDROL_F1_2coord: 44..58
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 34..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G005000.1Lsi06G005000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds