Lsi06G003660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G003660
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr06: 4024342 .. 4028751 (+)
RNA-Seq Expression	Lsi06G003660
Synteny	Lsi06G003660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGCCCAAAAAATACGACACACAAGGAGATTTTTGGGAGAATGTCTCGGCCGACCGTTGCTTCATCTTTTCCGGCGACGGTGGCGGTCGCATTGGCGGTGGTCCAACTCATCATCCTAATACTTCCGTCCGCCGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGATTCATCGAAGCTCGATGAATGGATCGCACACAATATGAGAGAATACAACGATCGTAAGGCGAACGAGACAGGTCTCAAGGCGCTGGACCGACGGCTGGCAAAGGCGGAGGACCACGTTCGACTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCACGACGATCACAGAAGCGATCGAGAGCATTCCGTCTGGAAATCGGCGGCGAGTTGTGGTGTGGATCGGCGGCGGAGTTTACAGAGAGAAGATCACGATCGAAGCTTCGAAGCCGTTCGTGACGTTGTATGGTCAGAAAGGAAAACGGCCGGTGATAACGTTCGACGGTACGGCATCGGAATTTGGTACCGTTAAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTGCGTTTGTGGTGCGTTTCTTCTTCTTCTTCTTTTCATTTTTGTGGGGTAAGTTTTGTAAATAGTAATAATTATGGGGCTATAAGTTTTGGCAACGTATGATGTGCATGAGTTTTATTGTTTTTTATTTTTATGAAAAAAAGAAAAAAAAATTCATGCATTCACTCGTCATATACTCATGCCATTCCTCATGTGTACAAAACATGATTTTTCACGTTGAAATCTTGACTTTTTCATGCACGATTGGTTAGTACTTTTTCTTTTTTCTTATACATATGAAAAAAATTAGTTTGAAACTATTTACAAATATAGAAAAATTTTACAATCTAACAGTAATAAATCGTGATAGAATTCTACCACTTGAGCGCGATAGAATTCTATCACTGTCTATCGTTGATAGACAATAAAATTTTTCTATATTTGTAAATAATTTGGCTATTTTTCTATATTGGAAAACCCAAAAAATCATTTAACGAATATTAGATGATATATAATTAAATTTACCTTTACCCATTAACTTAAGCTTTTGAATCAATCAGTAATTTAAGACCTTATAATATGTTTAGTGTGAATTAAAAAATTTGTATATCTCAAATAACTACGAGATATAAAGTGATGAGTTGGTAAGGAATTTTATACATATTATTGTGCCAGATCTTTATTCTCGTTTAGTAATAATTTTGTTGTTATTTATTTTTTGAAATTTATAATTGTTTTCTTCCAGTTTTTAACTAAAATTTTCACATTTCTTAAAGAAACACTTTTATAGTCAGATACTATAAATAAATTCTATTGTTTGAAAACTATTTTTTTTTAATTCGAATTTTGGCTAGAAAGGATAGCAAAACATAGAAACTTTTAGGTAAAAATAGTATTTTTAAGTTTAATTTTGGTTAAATTACAAATTTGGTTAATTCCAATGGTTTGGAAAAAGTTTAGAATTTAGTCCTTATAGTTTTAAAAGTTAAAGTTTAATTAGTCCATATATGTTGATAAAACCTCATAATTAAGTAGTCCCCTGGTTTGATAAAATCCTCCTAAATTCCTATGAGAATTTTATCAAATCATAGAGATTAAATTCTAATTATAAAGTATCGGGATTAAATTTTAACTTTCTTTCACCAAACCATAAAAAACCATTTTTTTTAAAAAAAAATACAATAGGGGATAGCAATCCTAACATGTTTACTTGTGATGACCTCTTAAACAAAGAAGAGAAAGGAATTGAAATTAAAGTTGGCTTCATTCCATTTAAAAGCAGGCAATGAGTTTGGTGTAACATTTTTAGTATATGGTAAAGTACTTCATTTCTTAGAATTGGAAAAAGTTGATATGTATAAAAGAGGCAAACTAAAGGAATTTATATATTGACAAATAACACCATGAGAGAAGTAACTCGTGTATCTTATAAATGTCTTGTTAAGATATTGTCTCTTTAGAGCTCACCATTCTTGAGTTGCATCCTCCTTTTTTTAGGTCAAAAACCCGTTTGGACTTCTTGGACTTTAATACCATATTAGATAAACACGAGGTTCCATCTTAAAAGCATCTGACAATAGGAAAAGTAATCTATATATATCTTATAAAAATTTGAGATCTCTTCAACATTTTAATGTGGAATTCTCAACCATGATTACTCATTAATAAATTAGTTTATATTTGATTAAGAAGTGAAGTTTGGTTGTTAAATTTGTTGTGCAGAATTCAGCTCCAATGCCTGACTTAGGAGGAACAGGAGGGCAAGCAGTGGCAATGAGGATATCTGGGGATAAAGCAGCATTCCACGAGTGTCATTTCATAGGCTTTCAAGATACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGACTGTTACATCCAAGGCACTGTGGATTTCATCTTTGGAAATGGCAAATCTCTCTATTTGGTATGATACATCCTTTATACTTTAAAATGTTCGTAAACTTACCTAACATGATACTTAGCGATCAGCTAATAGAAATTCAGATCAGCAATTAAGTCAATTTTAGGAGAAGAAAATATCCAAATTGGTAGGAATGGTGCGTTTCACGATAATTTAGTCGATAGTTTCCATCGTTGGATGTATGGAAAAGAAGTACTTCAAAGTTATATCCAATAGTTATTAAGAGATATGTATGTTTTTGAATCTACGTCTAACGATTGTGTGACAGTCTCATTGAATAATTTCTACTCCTTGATGAGTCATGAGGTGACAAGTTTCATCCTACGAAAAATGATATGATGATGTCTAAATATTAAAGACTAAAACGTATATGACGACAGGTTCAATTGGACATATAAGAAAGAAGTACCTTGGATTTACATCTAATAGTTATTAATGGGTATGCATGTTTCTGAATCTACGTCTAACGATTGTGTAGCGTCACATCCACATTGATTAATTCCTCCTTCTTGATAAGTCAGGAGGGCGACAAGTTTTGTCGTACGAAAAATGATATGACGATATCTAAACATTAAAGACTAAAACATATATTTAGTATATCTATTTCTGTCTCTCTTTCAAAGAGAAAGGTAATAGGCAATGATCATTTCATCTATACAGAAAACCACAATAAAATCAGTTGCAGAAGGGACAGGTGTGATAACAGCTCAAGCAAGGGAAGAAGCCACAGATGACAGTGGTTTCACATTTGCATATTGCAACATCACAGGAACAGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTAGTTTTTGCTTACACATATATGGGAACTCTCATTAACACAGAAGGATGGTCTGACAAAATGCACGGAAGCCAACCTAGAAAGTAAGCAAAAAAATCACACCTGATATCTTTATACAATTAAGATATGTATTCTAACGAGTCGACTAATTATCATCTTAATTTTCGAATGTATGTGAACACAGGAGTATGTACTACGGCGAGTACAAATGCATGGGACCTGGGGCAACCCCTTCGGGTCGAGTGAAGTATGCTAGAATACTCTCGGACGAAGAAGCGAAAGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGCTTCTACCACCCCCAGATCTTTCCCTCTCCCATTCTCAACCCAATTTCTTCCATTAAAATTGAATGAAATGCTGCATTTTATGCTTTATCAAAAAGGAAGTTAATACTATATATCCCAGAATGTGGCCATAAAACCATGAAAGTAACTAATAGCGATAATTAGAAAAAGCAGGGCCTAGAACTTTATCCCATATATTTCCCCATTTAAATGTCTGTCACATAGAAATAATCCCATTTTTTTTGGTCCAAAGTTTTAGTTGATTGTTGTACCAAACTGATCAACTTTTTTTATTTTTATTTTTATTTTTTATTTTTATTTTTTTGCTGACTCTCATAATCTCTCCTATGTTATGGTCGTTTTTGCAAGGGAAAAGTACATGATCATGCAATGTTGTTGTTTATGAGATTGTGTAGCTATTTTTCTTCCAACACTAATAATTGTGTATGGTGAAATTTTGAGATACAAAACCTACTCTAAATGTGTTTGGTATCAAAGCTTTACTAGAAATGGTCTCTTTTAAAAGCTTCTATTATCTTTTCTTTATCATTTGAACAATCCCTATTCTTTGCCACATCTGGCTGGAGTGGAACAATCGAACCTTCAACAACTACAGGAATTGTTCTATTGATAGTACTTGCTTGAACGTTCGTCCTTTCTGCGACTACTTGGCCTACAAAATTTTTTCTTTTTGTTAATTATCCTTATTCTTGTATTGCCTTAAACTGGCACATGTTCAATGTAACTCCTCTTGTTTCGTATGTACTTTATACATACAGGCTCCTTTGTAAAGTATCTTCTTTT

mRNA sequence

CAAGCCCAAAAAATACGACACACAAGGAGATTTTTGGGAGAATGTCTCGGCCGACCGTTGCTTCATCTTTTCCGGCGACGGTGGCGGTCGCATTGGCGGTGGTCCAACTCATCATCCTAATACTTCCGTCCGCCGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGATTCATCGAAGCTCGATGAATGGATCGCACACAATATGAGAGAATACAACGATCGTAAGGCGAACGAGACAGGTCTCAAGGCGCTGGACCGACGGCTGGCAAAGGCGGAGGACCACGTTCGACTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCACGACGATCACAGAAGCGATCGAGAGCATTCCGTCTGGAAATCGGCGGCGAGTTGTGGTGTGGATCGGCGGCGGAGTTTACAGAGAGAAGATCACGATCGAAGCTTCGAAGCCGTTCGTGACGTTGTATGGTCAGAAAGGAAAACGGCCGGTGATAACGTTCGACGGTACGGCATCGGAATTTGGTACCGTTAAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTGCGTTTGTGAATTCAGCTCCAATGCCTGACTTAGGAGGAACAGGAGGGCAAGCAGTGGCAATGAGGATATCTGGGGATAAAGCAGCATTCCACGAGTGTCATTTCATAGGCTTTCAAGATACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGACTGTTACATCCAAGGCACTGTGGATTTCATCTTTGGAAATGGCAAATCTCTCTATTTGAAAACCACAATAAAATCAGTTGCAGAAGGGACAGGTGTGATAACAGCTCAAGCAAGGGAAGAAGCCACAGATGACAGTGGTTTCACATTTGCATATTGCAACATCACAGGAACAGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTAGTTTTTGCTTACACATATATGGGAACTCTCATTAACACAGAAGGATGGTCTGACAAAATGCACGGAAGCCAACCTAGAAAGAGTATGTACTACGGCGAGTACAAATGCATGGGACCTGGGGCAACCCCTTCGGGTCGAGTGAAGTATGCTAGAATACTCTCGGACGAAGAAGCGAAAGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGCTTCTACCACCCCCAGATCTTTCCCTCTCCCATTCTCAACCCAATTTCTTCCATTAAAATTGAATGAAATGCTGCATTTTATGCTTTATCAAAAAGGAAGTTAATACTATATATCCCAGAATGTGGCCATAAAACCATGAAAGTAACTAATAGCGATAATTAGAAAAAGCAGGGCCTAGAACTTTATCCCATATATTTCCCCATTTAAATGTCTGTCACATAGAAATAATCCCATTTTTTTTGGTCCAAAGTTTTAGTTGATTGTTGTACCAAACTGATCAACTTTTTTTATTTTTATTTTTATTTTTTATTTTTATTTTTTTGCTGACTCTCATAATCTCTCCTATGTTATGGTCGTTTTTGCAAGGGAAAAGTACATGATCATGCAATGTTGTTGTTTATGAGATTGTGTAGCTATTTTTCTTCCAACACTAATAATTGTGTATGGTGAAATTTTGAGATACAAAACCTACTCTAAATGTGTTTGGTATCAAAGCTTTACTAGAAATGGTCTCTTTTAAAAGCTTCTATTATCTTTTCTTTATCATTTGAACAATCCCTATTCTTTGCCACATCTGGCTGGAGTGGAACAATCGAACCTTCAACAACTACAGGAATTGTTCTATTGATAGTACTTGCTTGAACGTTCGTCCTTTCTGCGACTACTTGGCCTACAAAATTTTTTCTTTTTGTTAATTATCCTTATTCTTGTATTGCCTTAAACTGGCACATGTTCAATGTAACTCCTCTTGTTTCGTATGTACTTTATACATACAGGCTCCTTTGTAAAGTATCTTCTTTT

Coding sequence (CDS)

ATGTCTCGGCCGACCGTTGCTTCATCTTTTCCGGCGACGGTGGCGGTCGCATTGGCGGTGGTCCAACTCATCATCCTAATACTTCCGTCCGCCGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGATTCATCGAAGCTCGATGAATGGATCGCACACAATATGAGAGAATACAACGATCGTAAGGCGAACGAGACAGGTCTCAAGGCGCTGGACCGACGGCTGGCAAAGGCGGAGGACCACGTTCGACTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCACGACGATCACAGAAGCGATCGAGAGCATTCCGTCTGGAAATCGGCGGCGAGTTGTGGTGTGGATCGGCGGCGGAGTTTACAGAGAGAAGATCACGATCGAAGCTTCGAAGCCGTTCGTGACGTTGTATGGTCAGAAAGGAAAACGGCCGGTGATAACGTTCGACGGTACGGCATCGGAATTTGGTACCGTTAAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTGCGTTTGTGAATTCAGCTCCAATGCCTGACTTAGGAGGAACAGGAGGGCAAGCAGTGGCAATGAGGATATCTGGGGATAAAGCAGCATTCCACGAGTGTCATTTCATAGGCTTTCAAGATACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGACTGTTACATCCAAGGCACTGTGGATTTCATCTTTGGAAATGGCAAATCTCTCTATTTGAAAACCACAATAAAATCAGTTGCAGAAGGGACAGGTGTGATAACAGCTCAAGCAAGGGAAGAAGCCACAGATGACAGTGGTTTCACATTTGCATATTGCAACATCACAGGAACAGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTAGTTTTTGCTTACACATATATGGGAACTCTCATTAACACAGAAGGATGGTCTGACAAAATGCACGGAAGCCAACCTAGAAAGAGTATGTACTACGGCGAGTACAAATGCATGGGACCTGGGGCAACCCCTTCGGGTCGAGTGAAGTATGCTAGAATACTCTCGGACGAAGAAGCGAAAGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGCTTCTACCACCCCCAGATCTTTCCCTCTCCCATTCTCAACCCAATTTCTTCCATTAA

Protein sequence

MSRPTVASSFPATVAVALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH

Homology

[Close]

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.7e-96
Identity = 170/364 (46.70%), Postives = 246/364 (67.58%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           ++++VV  +++     V+   T  IP + +++ +W   N++ Y+ RK        LD  L
Sbjct: 4   ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   ++ITV + G  NF T+ EAI+SIP+GN+ RV++ +  GVY EK+TI+ ++PF+
Sbjct: 64  EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ G   V+T+ GTA+++GTV+SAT+ V ++YF A +L   N+APMP   G+ GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VITAQ R+ AT+ +G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N 
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW + ++     K+++YGEYKC GPG+    RV Y + +   E   FL++ YI G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359

Query: 376 LPPP 379
           LPPP
Sbjct: 364 LPPP 359

[Close]

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 350.5 bits (898), Expect = 2.4e-95
Identity = 174/364 (47.80%), Postives = 236/364 (64.84%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V+L V  L+++I    V       IP +  ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
                      V+++GRG+F TITEAI S+ +GN RRV++ IG GVY+EK+TI+ SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TLYG     PV+TFDGTA+++GTV SAT+ V SDYF+AVN+   NSAPMPD    G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+KAAF+ C F G+QDT+CDD G HFFKDCYI+GT DFIFG+G+SLYL T +  V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VITA A + A + SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +     +  K+++YGEYKC G G+    RVKY + + D EAK F+S+ YI G+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 376 LPPP 379
           LPPP
Sbjct: 366 LPPP 336

[Close]

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 2.1e-91
Identity = 170/366 (46.45%), Postives = 239/366 (65.30%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V++ +  L+I + P    +  T  IP    ++ +W       +N         K LD  L
Sbjct: 6   VSILLGMLVIFVSPMVFADDLT-PIPEGKPQVVQW-------FNTHVGPLAQRKGLDPAL 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G YREK+TI+ +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A A + A + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEYKC GPG+  + RV + + + D+EA  FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361

Query: 376 LPPPDL 381
           LPPP L
Sbjct: 366 LPPPAL 361

[Close]

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.1e-91
Identity = 163/364 (44.78%), Postives = 242/364 (66.48%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           ++LA+V L++      V+      IPAD +++ +W   N++ ++ R+        LD  L
Sbjct: 4   ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V ++G G+F TI  AI+SIP  N+ RV++ +  G+Y EK+T++  +P+V
Sbjct: 64  EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL G+ G    +T+ GTA+++GTV+SAT+ V +  F+A NL  +N++PMP   GT GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           AMRI+GDKAAF+ C F GFQDTLCDDRG HFFK+CYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A  R+  T+ +G++F +C +TG G   YLGRAW    +VV++YT M +++N 
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEY C GPG+  + RV + + + ++EA  FL++ YI G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359

Query: 376 LPPP 379
           LPPP
Sbjct: 364 LPPP 359

[Close]

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 6.2e-91
Identity = 169/366 (46.17%), Postives = 240/366 (65.57%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V++ +  L++ + P    +  T  IP    ++ +W   N+     R       K LD  L
Sbjct: 6   VSILLGLLMVFVTPMVFADDVT-PIPEGKPQVAQWFNANVGPLAQR-------KGLDPAL 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G Y+EK+TI+ +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A A + A + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEYKC GPG+  + RV + + + D+EA  FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWL 361

Query: 376 LPPPDL 381
           LPPP L
Sbjct: 366 LPPPAL 361

[Close]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.3e-205
Identity = 365/394 (92.64%), Postives = 377/394 (95.69%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVV-QLIILI-LPSAV-VEARTKTIPADSSKLDEWIAHNMRE 60
           M RPTVASSF ATVA+ALAV+ QLIILI +PS V VEARTKTIPADSSKLDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETG+KALDRRLA+AED VRLITVRKDGRGNF+TITEAIESIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180
           GGGVYREKITI+ASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+LGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFF+DCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTI SVAEG GVITAQARE+ATD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGAT SGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           EAKAFLSMTYIHGNKWLLPPPDLS S S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

[Close]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 7.3e-204
Identity = 357/394 (90.61%), Postives = 375/394 (95.18%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAV-VQLIILIL--PSAVVEARTKTIPADSSKLDEWIAHNMRE 60
           M RPTVASSFPATVA A+ V +QLIILI+   + VVEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETG+KALDRRLA+AED V+LITVRKDGRGNF+TITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180
           GGGVYREKITI+ASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+LGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTI SVAEGTGVITAQARE+ATD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGATPSGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           EAKAFLSMTYIHGNKWLLPPPDLSLS S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

[Close]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 332/391 (84.91%), Postives = 356/391 (91.05%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYND 60
           M RP VASS P  VA+ALAV+QLII ILP+AVV ARTK IPA+ SKLDEWI +NM+EY++
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLII-ILPAAVV-ARTKPIPAEPSKLDEWIENNMKEYHE 60

Query: 61  RKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGG 120
           RKAN+TG+KALD+RLA AED VRLITVR DGRGNFTTIT AIESIP+GN  RV+VWI GG
Sbjct: 61  RKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGG 120

Query: 121 VYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180
           +YREKITI+ASKPFVTLYGQ GK PVITFDGTASEFGTV+SATVAVESDYFVAVN+AFVN
Sbjct: 121 IYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVN 180

Query: 181 SAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240
           SA MPD+  +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCYI+GTVDFIF
Sbjct: 181 SAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIF 240

Query: 241 GNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300
           GNGKSLYLKT I SVAEG GVITAQARE+ATDDSGFTF YCNITGTGNTYLGRAWKERTR
Sbjct: 241 GNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTR 300

Query: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360
           VVFAYTYMG LINTEGWSDKMH  +PRKSMYYGEYKCMGPGATP+GRVKYARILSDEEA+
Sbjct: 301 VVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEAR 360

Query: 361 AFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 AFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

[Close]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.4e-186
Identity = 324/383 (84.60%), Postives = 351/383 (91.64%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSA-VVEARTKTIPADSSKLDEWIAHNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI +++P+A VVEARTKTIPA+ S+LDEWIAHNMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGG 120
            RKANETG K LD RLAKAED  RLITV++DG+GNF+TIT+AIESIPSGNR RVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFV 180
           G YREKITI++SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE +TDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEA 360
           RVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKC GPGA  +GR  Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWLLPPPDLSL 383
           KAFLSMTYIHGNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

[Close]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.5e-185
Identity = 323/383 (84.33%), Postives = 351/383 (91.64%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSA-VVEARTKTIPADSSKLDEWIAHNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI +++P+A VVEARTK IPA+ S+LDEWIAHNMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGG 120
            RKA ETG KALDRRLAKAED  RLITV++DG+GNF+TIT+AIESIPSGN  RVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFV 180
           G YREKITI++SKPFVTLYGQ GK PVI++DGTASEFGTV+SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE +TD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEA 360
           RVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKC GPGA  +GR  Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWLLPPPDLSL 383
           KAFLSMTYIHGNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

[Close]

BLAST of Lsi06G003660 vs. NCBI nr
Match: XP_038880021.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 744.6 bits (1921), Expect = 4.4e-211
Identity = 367/391 (93.86%), Postives = 380/391 (97.19%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYND 60
           MSRPTVASS PA VAV+LA+ QLIILI+ SAVVEARTK IPAD+SKLD+WIAHNM+EY +
Sbjct: 1   MSRPTVASSSPAAVAVSLALFQLIILIISSAVVEARTKMIPADASKLDQWIAHNMKEYEE 60

Query: 61  RKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGG 120
           RKANETG+KALDRRLAKAEDHVRLITVRKDGRGNFTTIT+AIESIPSGNRRRVVVWIGGG
Sbjct: 61  RKANETGIKALDRRLAKAEDHVRLITVRKDGRGNFTTITKAIESIPSGNRRRVVVWIGGG 120

Query: 121 VYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180
           VYREKITIEASKPFVTLYGQKG+RPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN
Sbjct: 121 VYREKITIEASKPFVTLYGQKGRRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180

Query: 181 SAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240
           SAPMP+LGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF
Sbjct: 181 SAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240

Query: 241 GNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300
           GNGKSLYLKT I SVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR
Sbjct: 241 GNGKSLYLKTIINSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300

Query: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360
           VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK
Sbjct: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360

Query: 361 AFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           AFLSMTYIHGNKWLLPPP+LSL HS+ N FH
Sbjct: 361 AFLSMTYIHGNKWLLPPPNLSLFHSRSNVFH 391

[Close]

BLAST of Lsi06G003660 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 724.5 bits (1869), Expect = 4.7e-205
Identity = 365/394 (92.64%), Postives = 377/394 (95.69%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVV-QLIILI-LPSAV-VEARTKTIPADSSKLDEWIAHNMRE 60
           M RPTVASSF ATVA+ALAV+ QLIILI +PS V VEARTKTIPADSSKLDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETG+KALDRRLA+AED VRLITVRKDGRGNF+TITEAIESIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180
           GGGVYREKITI+ASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+LGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFF+DCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTI SVAEG GVITAQARE+ATD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGAT SGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           EAKAFLSMTYIHGNKWLLPPPDLS S S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

[Close]

BLAST of Lsi06G003660 vs. NCBI nr
Match: XP_004144054.1 (putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Csa_007174 [Cucumis sativus])

HSP 1 Score: 719.5 bits (1856), Expect = 1.5e-203
Identity = 357/394 (90.61%), Postives = 375/394 (95.18%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAV-VQLIILIL--PSAVVEARTKTIPADSSKLDEWIAHNMRE 60
           M RPTVASSFPATVA A+ V +QLIILI+   + VVEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETG+KALDRRLA+AED V+LITVRKDGRGNF+TITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180
           GGGVYREKITI+ASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+LGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTI SVAEGTGVITAQARE+ATD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGATPSGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           EAKAFLSMTYIHGNKWLLPPPDLSLS S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

[Close]

BLAST of Lsi06G003660 vs. NCBI nr
Match: XP_022147590.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 669.1 bits (1725), Expect = 2.4e-188
Identity = 332/391 (84.91%), Postives = 356/391 (91.05%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYND 60
           M RP VASS P  VA+ALAV+QLII ILP+AVV ARTK IPA+ SKLDEWI +NM+EY++
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLII-ILPAAVV-ARTKPIPAEPSKLDEWIENNMKEYHE 60

Query: 61  RKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGG 120
           RKAN+TG+KALD+RLA AED VRLITVR DGRGNFTTIT AIESIP+GN  RV+VWI GG
Sbjct: 61  RKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGG 120

Query: 121 VYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180
           +YREKITI+ASKPFVTLYGQ GK PVITFDGTASEFGTV+SATVAVESDYFVAVN+AFVN
Sbjct: 121 IYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVN 180

Query: 181 SAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240
           SA MPD+  +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCYI+GTVDFIF
Sbjct: 181 SAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIF 240

Query: 241 GNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300
           GNGKSLYLKT I SVAEG GVITAQARE+ATDDSGFTF YCNITGTGNTYLGRAWKERTR
Sbjct: 241 GNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTR 300

Query: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360
           VVFAYTYMG LINTEGWSDKMH  +PRKSMYYGEYKCMGPGATP+GRVKYARILSDEEA+
Sbjct: 301 VVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEAR 360

Query: 361 AFLSMTYIHGNKWLLPPPDLSLSHSQPNFFH 392
           AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 AFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

[Close]

BLAST of Lsi06G003660 vs. NCBI nr
Match: XP_022961183.1 (putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinesterase 50 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.4 bits (1705), Expect = 4.9e-186
Identity = 324/383 (84.60%), Postives = 351/383 (91.64%), Query Frame = 0


Query: 1   MSRPTVASSFPATVAVALAVVQLIILILPSA-VVEARTKTIPADSSKLDEWIAHNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI +++P+A VVEARTKTIPA+ S+LDEWIAHNMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGLKALDRRLAKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGG 120
            RKANETG K LD RLAKAED  RLITV++DG+GNF+TIT+AIESIPSGNR RVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GVYREKITIEASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFV 180
           G YREKITI++SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+AFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDLGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTTIKSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE +TDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEA 360
           RVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKC GPGA  +GR  Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWLLPPPDLSL 383
           KAFLSMTYIHGNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

[Close]

BLAST of Lsi06G003660 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 353.2 bits (905), Expect = 2.7e-97
Identity = 170/364 (46.70%), Postives = 246/364 (67.58%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           ++++VV  +++     V+   T  IP + +++ +W   N++ Y+ RK        LD  L
Sbjct: 4   ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   ++ITV + G  NF T+ EAI+SIP+GN+ RV++ +  GVY EK+TI+ ++PF+
Sbjct: 64  EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ G   V+T+ GTA+++GTV+SAT+ V ++YF A +L   N+APMP   G+ GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VITAQ R+ AT+ +G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N 
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW + ++     K+++YGEYKC GPG+    RV Y + +   E   FL++ YI G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359

Query: 376 LPPP 379
           LPPP
Sbjct: 364 LPPP 359

[Close]

BLAST of Lsi06G003660 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.7e-96
Identity = 174/364 (47.80%), Postives = 236/364 (64.84%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V+L V  L+++I    V       IP +  ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
                      V+++GRG+F TITEAI S+ +GN RRV++ IG GVY+EK+TI+ SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TLYG     PV+TFDGTA+++GTV SAT+ V SDYF+AVN+   NSAPMPD    G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+KAAF+ C F G+QDT+CDD G HFFKDCYI+GT DFIFG+G+SLYL T +  V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VITA A + A + SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +     +  K+++YGEYKC G G+    RVKY + + D EAK F+S+ YI G+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 376 LPPP 379
           LPPP
Sbjct: 366 LPPP 336

[Close]

BLAST of Lsi06G003660 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-92
Identity = 170/366 (46.45%), Postives = 239/366 (65.30%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V++ +  L+I + P    +  T  IP    ++ +W       +N         K LD  L
Sbjct: 6   VSILLGMLVIFVSPMVFADDLT-PIPEGKPQVVQW-------FNTHVGPLAQRKGLDPAL 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G YREK+TI+ +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A A + A + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEYKC GPG+  + RV + + + D+EA  FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361

Query: 376 LPPPDL 381
           LPPP L
Sbjct: 366 LPPPAL 361

[Close]

BLAST of Lsi06G003660 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-92
Identity = 163/364 (44.78%), Postives = 242/364 (66.48%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           ++LA+V L++      V+      IPAD +++ +W   N++ ++ R+        LD  L
Sbjct: 4   ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V ++G G+F TI  AI+SIP  N+ RV++ +  G+Y EK+T++  +P+V
Sbjct: 64  EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL G+ G    +T+ GTA+++GTV+SAT+ V +  F+A NL  +N++PMP   GT GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           AMRI+GDKAAF+ C F GFQDTLCDDRG HFFK+CYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A  R+  T+ +G++F +C +TG G   YLGRAW    +VV++YT M +++N 
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEY C GPG+  + RV + + + ++EA  FL++ YI G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359

Query: 376 LPPP 379
           LPPP
Sbjct: 364 LPPP 359

[Close]

BLAST of Lsi06G003660 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-92
Identity = 169/366 (46.17%), Postives = 240/366 (65.57%), Query Frame = 0


Query: 16  VALAVVQLIILILPSAVVEARTKTIPADSSKLDEWIAHNMREYNDRKANETGLKALDRRL 75
           V++ +  L++ + P    +  T  IP    ++ +W   N+     R       K LD  L
Sbjct: 6   VSILLGLLMVFVTPMVFADDVT-PIPEGKPQVAQWFNANVGPLAQR-------KGLDPAL 65

Query: 76  AKAEDHVRLITVRKDGRGNFTTITEAIESIPSGNRRRVVVWIGGGVYREKITIEASKPFV 135
             AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G Y+EK+TI+ +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFI 125

Query: 136 TLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVNSAPMPDLGGTGGQAV 195
           TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTTIKSV 255
           +MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 256 AEGTGVITAQAREEATDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 315
            +G  VI A A + A + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 316 EGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAKAFLSMTYIHGNKWL 375
            GW +    +   K+++YGEYKC GPG+  + RV + + + D+EA  FLS+ YI G+KWL
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWL 361

Query: 376 LPPPDL 381
           LPPP L
Sbjct: 366 LPPPAL 361

The following BLAST results are available for this feature:

BLAST of Lsi06G003660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LY17	3.7e-96	46.70	Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1	[more]
Q9FKF3	2.4e-95	47.80	Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2	[more]
Q9LY19	2.1e-91	46.45	Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2	[more]
Q9LY18	2.1e-91	44.78	Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1	[more]
Q84WM7	6.2e-91	46.17	Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1	[more]

BLAST of Lsi06G003660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BRI5	2.3e-205	92.64	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1	[more]
A0A0A0M299	7.3e-204	90.61	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1	[more]
A0A6J1D0K0	1.1e-188	84.91	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1	[more]
A0A6J1HB45	2.4e-186	84.60	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1	[more]
A0A6J1JI92	1.5e-185	84.33	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1	[more]

BLAST of Lsi06G003660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880021.1	4.4e-211	93.86	putative pectinesterase 63 [Benincasa hispida]	[more]
XP_008450966.1	4.7e-205	92.64	PREDICTED: putative pectinesterase 63 [Cucumis melo]	[more]
XP_004144054.1	1.5e-203	90.61	putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Cs...	[more]
XP_022147590.1	2.4e-188	84.91	putative pectinesterase 63 [Momordica charantia]	[more]
XP_022961183.1	4.9e-186	84.60	putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinest...	[more]

BLAST of Lsi06G003660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07430.1	2.7e-97	46.70	Pectin lyase-like superfamily protein	[more]
AT5G61680.1	1.7e-96	47.80	Pectin lyase-like superfamily protein	[more]
AT5G07410.1	1.5e-92	46.45	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	1.5e-92	44.78	Pectin lyase-like superfamily protein	[more]
AT1G69940.1	4.4e-92	46.17	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 85..371 e-value: 2.2E-70 score: 236.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 80..377 e-value: 1.2E-103 score: 348.8
None	No IPR available	PANTHER	PTHR31321:SF30	PECTINESTERASE	coord: 19..379
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 19..379
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 232..241
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 84..375

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G003660.1	Lsi06G003660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity