Lsi06G000270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G000270
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
Locationchr06: 278269 .. 281962 (-)
RNA-Seq ExpressionLsi06G000270
SyntenyLsi06G000270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAAAAAAAGGGTTGCGATTACTTGGAAAGAGAAGAGAATGGAATCACCTTAGCTTAGTTGCAGTCATGGGTGCATGTGTGTACAAATGCACATGCATGTTTTCTTCTCTTGTAGTTGTGCTATGGTTGAATGTTACTTTATTATCTTTGAGGTTTTTTGTCTTTTGAGAAAAGATGGAAAAAGCAAAAAAGTATGGATGGCTAATCCCCTTTTTCTACTGTCATCAGAGGCAACATTTCCAATATCATCCTTGCTCATAAAGTAACAATTTTTTCCCCTTTCATTTATTAATAATCTATTTCTTTCTTTTATATTGCAGCCGTAAGCATTTGCAACTTGGTCATCTGTCTCTACTTTTTTCACCTTTACCTTCTTCCTTTTCCCCCCTTTCTCTTTCTCTTTCACTTTGCATGTTTCGCTCTTAACTCCTTTCCTTTTACACCATTTTCCCTGTCTGGTTTCGTTGTTTTTAAAAGCAATGCCTAAATTAAACCGATCCCATTAAGAATCTAGTTGGGTCTCTTCATTTGTCCCTAGAAGATAACTATAACCTCCCACCGATAAAATGGCCCAAACAGCTTCCAAATGCTTCTTCTTTTCCATTCTGTCTCTCCTCGCCATCTGCTCTTCTGGTAAGCAACTTCCTCTCTTCCAACTACAACACATTATAGATCTAGCTCAGTTCCTCGTTTTCTGCTCTGATTCTGTCTTGGTTCCTGCTACTTACAGCTTCTGAATCTTAACTTTGCATTTTTAGTGGCATTTCCTGTTTTGGCAAAAATAAAAAATTAAGGGTTTTTGTTTTAGTTCTGTTTCTGAATGCTTCTGCTTCTTCCATTCTCGCAGGTAGATTTGGTGGGTCGCATTTGGAAGTGGAAGAAAATGCTTCCACGGAGTTGAAATGGAAGTTCACTGAACTTGGTCGGACTACAATGCACGACGCCACAACAAATTTTCCGACAACCCCGGACACCTCCACGCCGACAATCATTACCGTTCCCTCGACCAACCCTGTCACTGTCACTCCGTCGAGCCCTGCTGCCACCCCGGTGTCCATTCCTCTAACGACACCCATCACAGTTCCGGCTAACTCTCCGGTGCCGCTCACCAATCCGGTTGCGCCACCGGTTACAGTCCCCGGAGCACAGCCCATAACGAACCCCGTAACCACGTATCCACCTCCGTCTGGTGGCGCTCCAGTGTTAACACCCGCCACAAATCCAGTTCCGGTGTCCCCTCCGGCGACGACGAACGCACCGATAATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCTGGCGCTTCAGAGATGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGCGGCCGATTGTTCTCAAATTCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTGAAAACCACGCTTCTTTTGCATTCAATAGCTATTTTCAGAAGAATCCTTCTTCTACAAGCTGCGACTTTGGAGGATCTGCAATGGTTACCAACTCCAATCCAAGTCAGAGAAGACGTTCATTGTTTCTCAATCTCCCACTTGATCCTCTGTTTTTCCGCAATTAATCCTCTTCTTTTCTTGTAACAGGCACTGGCTCTTGCATTTACCCATCATCATCTTCCTCTGCGACGCCGGCATCCATGACGCCGTCGGTTCCAACGCCGACATCGACAACAACAGCGCCGATCACCGTCTCACCAACTACAGTTACGAACCCAACAACGTCTTCACCCATAGGGACCGGCATGCCAGAGTAAGTGCTCTGTTCACTTCTCATGCCTTTGAATTCTTTGAAAAAGTTGAAACTTTTTTATTCATAGGGTTGAACTACACGTTTGGATAGTTTATAATATATCTTCAACTCTTAAAATGGTGTCCATATGATTTATCTAATTTATAATCTTCCGTTTCAACTTTTCTGTGTTTAATCAACTCAGATCGTTTAATACCTCTGCATATTTTGTTTTTTGTTTTAAAAAAATTAGGCTTGTTTTTTTACAAGATTTTCATAAATATTTGAATTTATTGTGAAATGGAAAAAAAGAACATTTTTTTTAGTTTGATAATCCTTTTGATTTTTGAAAATTGTGCTTCCATTCTCATATTTTTTTAACCATAGGTTTCATCTTTACTCAAAATTACTTTTTTTAATTTTTAAAATTTGTCATGGATTTTGAAAACACCGAGAATGTGGTGTTTATGGACTTCATTTTTAAAATTTAAAAATAAAAAACTAAATACTTATTAAATAAGATCTTAAGCCTGACTTGAATTTTGAAAACACTCTTGCAAAATAGATATAAAAACAGAGAAACTATAGGAGGTTTATAAGTTTTATTTTTTAGTTTATAAGCTTAATTTTAATCAAATAGTGATCAAGATTATCAAAGGAGAATTTAATAATCCATGATGTTGGTGCAGAAATGGAAGTCCTCCTGGAGTGTTTAACACAGACAATCCAGCTTCGAGCATAGGCAGCACAACAGGGTTTGGAACTGAGATTCCTCCAAGCTCAAGCACCTCAATATCCATGGCGGCTGGATTGAGACCCTTTCCTTGTTGCATCATTCTGACTATGTCCTTGATCACTCGCAGAATAATCACCCTGGACTGGTGATGAAATCACTGACTTCTTCTCCTTCTACTGTACATGAAAGACCCTCGTAATTCATAGCTTCAGAATCGCCGCCCAGTTCCACCATGAGTGCAATTTTTGGAGGAGAAAAGGAACCAGAGGTGAGTTTGGAAGGATAAACAACAAGGTTTACAGAATGCACTGCCACTGCCACTGCCACTGCTTGAGCTTTGGTTTGTGTAAAGATAAAAGATAGGGATGTATTAGCTACTTGCAACTTTGAGACTCTGTAATATAATATATTGTTAGATTTTGGGAGTATTATTGGGATTTGTGTTGCTGTTGGTCATAGGTGCATTAGGATTAGAATTTTGGTTAGAAGAAACAGAGACACATAGCTGGACCGGAAGTATTTCTATTCCTATGTTTTTAAGTCTCGAATGTTTTGTATGATTTAATGATTATTAATTGATTGTTTGTTTACGTATGAGTTTATAATTGCATGATGTCTGTGGTTGCTGAAATTATCTTAGTTGACTTGCTCTTCTCCAGCAAACAACAAGAATAATTGGAAGGAAGATTAAAAAACTTCTTCTCCAACAAGAATAATCTTCCTTTGGGTAAACATGCAGCCACACGAAAGCACTGTACCTTGATGATCATCCAACTGAGCCCTCCTCTATCTTCTTTACACGACCACACCGTGTCAACATTGAGCTTCCAACAGTCAACTTCAGGAGGACCTCCAATGATGATGAGACAGAACAGGAGGGAAAGGAAGAAGCGGAGGAAGCCTGACAAGGGGGCCAACCAAACATCCCATTCGACTCGTCCAACTCTTCCAAATGCTTTTCTATCGAGATTTTGAGAATTTTAATATCAAGGACCACTTCGTACACAAAGAGTAACTGATCGATGAATATCGTCCATCCACGAGACTGTAAAAAGAGAGAATGGGGTTTTAACCTAGTAGGGGTGTGAGGGCGCAGATGGAGAGTTTTTTGGTAGGGCACATACTCTCTTACAGTGAAAAGAGAAGAGTTAAGAACATTACTTGTTAGCTCGGTCAAATCAATGTCTAATTATCTTCTTTGTCTGAAAATGGGTT

mRNA sequence

AGAGAGAAAAAAAGGGTTGCGATTACTTGGAAAGAGAAGAGAATGGAATCACCTTAGCTTAGTTGCAGTCATGGGTGCATGTGTGTACAAATGCACATGCATGTTTTCTTCTCTTGTAGTTGTGCTATGGTTGAATGTTACTTTATTATCTTTGAGGTTTTTTGTCTTTTGAGAAAAGATGGAAAAAGCAAAAAAGTATGGATGGCTAATCCCCTTTTTCTACTGTCATCAGAGGCAACATTTCCAATATCATCCTTGCTCATAAAGTAACAATTTTTTCCCCTTTCATTTATTAATAATCTATTTCTTTCTTTTATATTGCAGCCGTAAGCATTTGCAACTTGGTCATCTGTCTCTACTTTTTTCACCTTTACCTTCTTCCTTTTCCCCCCTTTCTCTTTCTCTTTCACTTTGCATGTTTCGCTCTTAACTCCTTTCCTTTTACACCATTTTCCCTGTCTGGTTTCGTTGTTTTTAAAAGCAATGCCTAAATTAAACCGATCCCATTAAGAATCTAGTTGGGTCTCTTCATTTGTCCCTAGAAGATAACTATAACCTCCCACCGATAAAATGGCCCAAACAGCTTCCAAATGCTTCTTCTTTTCCATTCTGTCTCTCCTCGCCATCTGCTCTTCTGGTAGATTTGGTGGGTCGCATTTGGAAGTGGAAGAAAATGCTTCCACGGAGTTGAAATGGAAGTTCACTGAACTTGGTCGGACTACAATGCACGACGCCACAACAAATTTTCCGACAACCCCGGACACCTCCACGCCGACAATCATTACCGTTCCCTCGACCAACCCTGTCACTGTCACTCCGTCGAGCCCTGCTGCCACCCCGGTGTCCATTCCTCTAACGACACCCATCACAGTTCCGGCTAACTCTCCGGTGCCGCTCACCAATCCGGTTGCGCCACCGGTTACAGTCCCCGGAGCACAGCCCATAACGAACCCCGTAACCACGTATCCACCTCCGTCTGGTGGCGCTCCAGTGTTAACACCCGCCACAAATCCAGTTCCGGTGTCCCCTCCGGCGACGACGAACGCACCGATAATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCTGGCGCTTCAGAGATGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGCGGCCGATTGTTCTCAAATTCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTGAAAACCACGCTTCTTTTGCATTCAATAGCTATTTTCAGAAGAATCCTTCTTCTACAAGCTGCGACTTTGGAGGATCTGCAATGGTTACCAACTCCAATCCAAGCACTGGCTCTTGCATTTACCCATCATCATCTTCCTCTGCGACGCCGGCATCCATGACGCCGTCGGTTCCAACGCCGACATCGACAACAACAGCGCCGATCACCGTCTCACCAACTACAGTTACGAACCCAACAACGTCTTCACCCATAGGGACCGGCATGCCAGAAAATGGAAGTCCTCCTGGAGTGTTTAACACAGACAATCCAGCTTCGAGCATAGGCAGCACAACAGGGTTTGGAACTGAGATTCCTCCAAGCTCAAGCACCTCAATATCCATGGCGGCTGGATTGAGACCCTTTCCTTGTTGCATCATTCTGACTATGTCCTTGATCACTCGCAGAATAATCACCCTGGACTGGTGATGAAATCACTGACTTCTTCTCCTTCTACTGTACATGAAAGACCCTCGTAATTCATAGCTTCAGAATCGCCGCCCAGTTCCACCATGAGTGCAATTTTTGGAGGAGAAAAGGAACCAGAGGTGAGTTTGGAAGGATAAACAACAAGGTTTACAGAATGCACTGCCACTGCCACTGCCACTGCTTGAGCTTTGGTTTGTGTAAAGATAAAAGATAGGGATGTATTAGCTACTTGCAACTTTGAGACTCTGTAATATAATATATTGTTAGATTTTGGGAGTATTATTGGGATTTGTGTTGCTGTTGGTCATAGGTGCATTAGGATTAGAATTTTGGTTAGAAGAAACAGAGACACATAGCTGGACCGGAAGTATTTCTATTCCTATGTTTTTAAGTCTCGAATGTTTTGTATGATTTAATGATTATTAATTGATTGTTTGTTTACGTATGAGTTTATAATTGCATGATGTCTGTGGTTGCTGAAATTATCTTAGTTGACTTGCTCTTCTCCAGCAAACAACAAGAATAATTGGAAGGAAGATTAAAAAACTTCTTCTCCAACAAGAATAATCTTCCTTTGGGTAAACATGCAGCCACACGAAAGCACTGTACCTTGATGATCATCCAACTGAGCCCTCCTCTATCTTCTTTACACGACCACACCGTGTCAACATTGAGCTTCCAACAGTCAACTTCAGGAGGACCTCCAATGATGATGAGACAGAACAGGAGGGAAAGGAAGAAGCGGAGGAAGCCTGACAAGGGGGCCAACCAAACATCCCATTCGACTCGTCCAACTCTTCCAAATGCTTTTCTATCGAGATTTTGAGAATTTTAATATCAAGGACCACTTCGTACACAAAGAGTAACTGATCGATGAATATCGTCCATCCACGAGACTGTAAAAAGAGAGAATGGGGTTTTAACCTAGTAGGGGTGTGAGGGCGCAGATGGAGAGTTTTTTGGTAGGGCACATACTCTCTTACAGTGAAAAGAGAAGAGTTAAGAACATTACTTGTTAGCTCGGTCAAATCAATGTCTAATTATCTTCTTTGTCTGAAAATGGGTT

Coding sequence (CDS)

ATGGCCCAAACAGCTTCCAAATGCTTCTTCTTTTCCATTCTGTCTCTCCTCGCCATCTGCTCTTCTGGTAGATTTGGTGGGTCGCATTTGGAAGTGGAAGAAAATGCTTCCACGGAGTTGAAATGGAAGTTCACTGAACTTGGTCGGACTACAATGCACGACGCCACAACAAATTTTCCGACAACCCCGGACACCTCCACGCCGACAATCATTACCGTTCCCTCGACCAACCCTGTCACTGTCACTCCGTCGAGCCCTGCTGCCACCCCGGTGTCCATTCCTCTAACGACACCCATCACAGTTCCGGCTAACTCTCCGGTGCCGCTCACCAATCCGGTTGCGCCACCGGTTACAGTCCCCGGAGCACAGCCCATAACGAACCCCGTAACCACGTATCCACCTCCGTCTGGTGGCGCTCCAGTGTTAACACCCGCCACAAATCCAGTTCCGGTGTCCCCTCCGGCGACGACGAACGCACCGATAATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCTGGCGCTTCAGAGATGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGCGGCCGATTGTTCTCAAATTCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTGAAAACCACGCTTCTTTTGCATTCAATAGCTATTTTCAGAAGAATCCTTCTTCTACAAGCTGCGACTTTGGAGGATCTGCAATGGTTACCAACTCCAATCCAAGCACTGGCTCTTGCATTTACCCATCATCATCTTCCTCTGCGACGCCGGCATCCATGACGCCGTCGGTTCCAACGCCGACATCGACAACAACAGCGCCGATCACCGTCTCACCAACTACAGTTACGAACCCAACAACGTCTTCACCCATAGGGACCGGCATGCCAGAAAATGGAAGTCCTCCTGGAGTGTTTAACACAGACAATCCAGCTTCGAGCATAGGCAGCACAACAGGGTTTGGAACTGAGATTCCTCCAAGCTCAAGCACCTCAATATCCATGGCGGCTGGATTGAGACCCTTTCCTTGTTGCATCATTCTGACTATGTCCTTGATCACTCGCAGAATAATCACCCTGGACTGGTGA

Protein sequence

MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFPTTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIITLDW
Homology
BLAST of Lsi06G000270 vs. ExPASy Swiss-Prot
Match: Q93V72 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.8e-21
Identity = 78/174 (44.83%), Postives = 106/174 (60.92%), Query Frame = 0

Query: 166 SWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNP 225
           ++C+ + G +E  LQ A+DYACG G ADC+QIQ  G+CY PNT++NH   A NSY+QK  
Sbjct: 20  AYCLCKEG-NEQVLQKAIDYACGNG-ADCTQIQPTGACYQPNTVKNHCDVAVNSYYQKKA 79

Query: 226 SS-TSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTS---TTTAPITVSP 285
           SS  +CDF G+A  + + PST S     SSSS TP + TP+  TPTS   TT  P T +P
Sbjct: 80  SSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTSGTPTTGTPTTGTP 139

Query: 286 TTVTNPTTSSPI----GTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSS 332
           TT T PT+ +P      TG P  G+  G+ N++   +S  S+   GT + P+ S
Sbjct: 140 TTGT-PTSGTPTSGFPNTGTPNTGTNTGMPNSNGMPTSSSSSVFPGTTLGPTGS 190

BLAST of Lsi06G000270 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 60/122 (49.18%), Postives = 82/122 (67.21%), Query Frame = 0

Query: 166 SWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNP 225
           +WCV + G SE  LQ  LDYACG G ADC  I Q G C+NPNT+++H S+A NS+FQK  
Sbjct: 20  TWCVCKEGLSEAMLQKTLDYACGAG-ADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQKKG 79

Query: 226 SST-SCDFGGSAMVTNSNPSTGSCIYPSSSS---SATPASMTPSVPTPTSTTTAPITVSP 284
            S  +CDF G+A  + S+PS  +C +P+S+S   + TP + TPS   PT+T T P T++P
Sbjct: 80  QSLGTCDFAGTATFSASDPSYTTCPFPASASGSGTTTPVTTTPSTRVPTTTNTRPYTITP 139

BLAST of Lsi06G000270 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 102.1 bits (253), Expect = 1.4e-20
Identity = 55/104 (52.88%), Postives = 71/104 (68.27%), Query Frame = 0

Query: 151 VSPPATTNAPIIPGQSWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLE 210
           VS   T  A     Q++C+A  G     LQ+ALD+ACG G ++CS+IQ G SCY PN ++
Sbjct: 365 VSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVK 424

Query: 211 NHASFAFNSYFQK-NPSSTSCDFGGSAMVTNSNPSTGSCIYPSS 254
            HASFAFNSY+QK   +S SCDF G AM+T ++PS GSCI+P S
Sbjct: 425 GHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIFPGS 468

BLAST of Lsi06G000270 vs. ExPASy Swiss-Prot
Match: Q9FZD0 (Carbohydrate-binding X8 domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At1g26450 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.2e-17
Identity = 79/196 (40.31%), Postives = 109/196 (55.61%), Query Frame = 0

Query: 168 CVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSS 227
           CV +  A+E+ LQ  +D+ACG G ADC+QIQ  G+CY PNTL+NH   A NSY+QK  S+
Sbjct: 22  CVCKD-ANELDLQKVIDFACG-GGADCAQIQTTGACYQPNTLKNHCDVAVNSYYQKKAST 81

Query: 228 -TSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTN 287
             +CDF G+A+++ S PST S    SSSS+ TP +  PS  T  STT +P T      TN
Sbjct: 82  GATCDFNGAAVISTSPPSTTSSCLSSSSSNGTPTAGYPS--TGNSTTASPGT------TN 141

Query: 288 PTTSSPIGTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAG----LRP 347
           P+T +   + +P N         D P SS   T    T + PSSSTS     G    +R 
Sbjct: 142 PSTGNSTNSTLPTN---------DKPTSST-ITFPDSTTMGPSSSTSGDPNGGEELSVRT 197

Query: 348 FPCCIILTMSLITRRI 359
               ++ T++ +  R+
Sbjct: 202 TTIILLTTIAAVALRV 197

BLAST of Lsi06G000270 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.2e-17
Identity = 70/190 (36.84%), Postives = 104/190 (54.74%), Query Frame = 0

Query: 166 SWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNP 225
           SWCV ++G S+  LQ+ LDYACG G ADC+  +   SC+NP+ + +H ++A NS+FQK  
Sbjct: 20  SWCVCKTGLSDTVLQATLDYACGNG-ADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKKG 79

Query: 226 SST-SCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTV 285
            S  SC+F G+A  TNS+PS   C +P+S+S ++            STT  P T +P   
Sbjct: 80  QSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSS-----------GSTTVTPGTTNPKGS 139

Query: 286 TNPTTSSPIGTGMPENGSPP-GVFNTDNPASSIGSTTGFGTEIP-PSSSTSISMAAGLRP 345
              TT    GT  P +G+P  GVF      +S G TTG G      + S++ ++    + 
Sbjct: 140 PTTTTLPGSGTNSPYSGNPTNGVFG----GNSTGGTTGTGINPDYTTDSSAFALKNSSKL 193

Query: 346 FPCCIILTMS 353
           F C +++  S
Sbjct: 200 FICLLLIASS 193

BLAST of Lsi06G000270 vs. ExPASy TrEMBL
Match: A0A0A0M1B6 (Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus OX=3659 GN=Csa_1G560700 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.9e-178
Identity = 343/363 (94.49%), Postives = 349/363 (96.14%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQTASKCFFFSI+SLLAICSSGRFGGSHL VEENAS+ELKWKF E GRTTMHDATTNFP
Sbjct: 1   MAQTASKCFFFSIMSLLAICSSGRFGGSHLGVEENASSELKWKFPEFGRTTMHDATTNFP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TTPDTSTPTIITVPSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           GAQPITNPVTTYP PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQ
Sbjct: 121 GAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTG ADCSQIQQ GSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGGADCSQIQQSGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+GTGMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGTGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIIT
Sbjct: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISVAAGLRPFTCFIILTMSFITHRIIT 360

Query: 361 LDW 364
           LDW
Sbjct: 361 LDW 363

BLAST of Lsi06G000270 vs. ExPASy TrEMBL
Match: A0A5A7U6N8 (Glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004640 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.0e-175
Identity = 339/362 (93.65%), Postives = 345/362 (95.30%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQ ASKCFFFSI+SLLAICSSGRFGGSHL VEEN S ELKWKF E GRTTMHDATTNFP
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKWKFIEFGRTTMHDATTNFP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TTPDTSTPTIITVPSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           GAQPITNPVTTYP PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQ
Sbjct: 121 GAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+G+GMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGSGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPG FNTDNPASSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIIT
Sbjct: 301 GSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGLRPFTCFIILTMSFITHRIIT 360

Query: 361 LD 363
           LD
Sbjct: 361 LD 362

BLAST of Lsi06G000270 vs. ExPASy TrEMBL
Match: A0A1S3BQE9 (glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo OX=3656 GN=LOC103492098 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.0e-175
Identity = 339/362 (93.65%), Postives = 345/362 (95.30%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQ ASKCFFFSI+SLLAICSSGRFGGSHL VEEN S ELKWKF E GRTTMHDATTNFP
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKWKFIEFGRTTMHDATTNFP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TTPDTSTPTIITVPSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           GAQPITNPVTTYP PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQ
Sbjct: 121 GAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+G+GMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGSGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPG FNTDNPASSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIIT
Sbjct: 301 GSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGLRPFTCFIILTMSFITHRIIT 360

Query: 361 LD 363
           LD
Sbjct: 361 LD 362

BLAST of Lsi06G000270 vs. ExPASy TrEMBL
Match: A0A6J1HC94 (mucin-2 OS=Cucurbita moschata OX=3662 GN=LOC111462029 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.5e-166
Identity = 327/362 (90.33%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDA----T 60
           MAQTASKCFFFS+LSLLAICSSGRFGGSHL+V+ENAS EL+WKF+EL RTTMHDA    T
Sbjct: 1   MAQTASKCFFFSVLSLLAICSSGRFGGSHLDVQENASAELEWKFSELVRTTMHDATDSPT 60

Query: 61  TNFPTTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPP 120
           TNFPTTPDTSTPTIITVP+TNP+TVTPSSPA+TPVSIPLTTPITVPANSPVPLTNPVAPP
Sbjct: 61  TNFPTTPDTSTPTIITVPATNPITVTPSSPASTPVSIPLTTPITVPANSPVPLTNPVAPP 120

Query: 121 VTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASE 180
           VTVPGAQPITNPVTTYP PSGG+PV+TP TNPVPVSPPA TNAP+IPGQSWCVARSGASE
Sbjct: 121 VTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVARSGASE 180

Query: 181 MALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSA 240
           MALQSALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPS TSCDFGGSA
Sbjct: 181 MALQSALDYACGTG-ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSCDFGGSA 240

Query: 241 MVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTG 300
           MVTNSNPSTGSC+YPSSSSSATPASMTPSVPTPT TTTAPITV+PT VTNP TSSP+GTG
Sbjct: 241 MVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TSSPVGTG 300

Query: 301 MPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITR 359
           MPENG+PP VFN DNPASSIGS+TGFGTEIPPSSSTSISMAAGLRPF  CII+TMSLITR
Sbjct: 301 MPENGTPPAVFNPDNPASSIGSSTGFGTEIPPSSSTSISMAAGLRPFTWCIIMTMSLITR 360

BLAST of Lsi06G000270 vs. ExPASy TrEMBL
Match: A0A6J1HUD2 (mucin-2 OS=Cucurbita maxima OX=3661 GN=LOC111467890 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.6e-166
Identity = 327/363 (90.08%), Postives = 344/363 (94.77%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDA----T 60
           MAQTASKCFFFSILSLLAICSSGRFGGSHL+V+ENAS EL+WKF+EL RTTMHDA    T
Sbjct: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLDVQENASAELEWKFSELVRTTMHDATNSPT 60

Query: 61  TNFPTTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPP 120
           TNFPTTPDTSTPTIITVP+TNPVTVTPSSPA+TPVSIPLTTPITVPANSPVPLTNPVAPP
Sbjct: 61  TNFPTTPDTSTPTIITVPATNPVTVTPSSPASTPVSIPLTTPITVPANSPVPLTNPVAPP 120

Query: 121 VTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASE 180
           VTVPGAQPITNPVTTYP PSGG+PV+TP TNPVPVSPPA TNAP+IPGQSWCV RSGASE
Sbjct: 121 VTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVVRSGASE 180

Query: 181 MALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSA 240
           MALQSALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPS TSCDFGGSA
Sbjct: 181 MALQSALDYACGTG-ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSCDFGGSA 240

Query: 241 MVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTG 300
           MVTNSNPSTGSC+YPSSSSSATPASMTPSVPTPT TTTAPITV+PT VTNP TSSP+GTG
Sbjct: 241 MVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TSSPVGTG 300

Query: 301 MPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITR 360
           MPENG+PP +FN DNP+SSIGS+TGFGTEIPPSSSTSISMAAGLRPF  CII+TMSLITR
Sbjct: 301 MPENGTPPAIFNPDNPSSSIGSSTGFGTEIPPSSSTSISMAAGLRPFTWCIIMTMSLITR 360

BLAST of Lsi06G000270 vs. NCBI nr
Match: XP_004135574.1 (mucin-5AC [Cucumis sativus])

HSP 1 Score: 633.6 bits (1633), Expect = 1.0e-177
Identity = 343/363 (94.49%), Postives = 349/363 (96.14%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQTASKCFFFSI+SLLAICSSGRFGGSHL VEENAS+ELKWKF E GRTTMHDATTNFP
Sbjct: 1   MAQTASKCFFFSIMSLLAICSSGRFGGSHLGVEENASSELKWKFPEFGRTTMHDATTNFP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TTPDTSTPTIITVPSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           GAQPITNPVTTYP PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQ
Sbjct: 121 GAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTG ADCSQIQQ GSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGGADCSQIQQSGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+GTGMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGTGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIIT
Sbjct: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISVAAGLRPFTCFIILTMSFITHRIIT 360

Query: 361 LDW 364
           LDW
Sbjct: 361 LDW 363

BLAST of Lsi06G000270 vs. NCBI nr
Match: XP_008450517.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 4 [Cucumis melo] >KAA0050964.1 glucan endo-1,3-beta-glucosidase 4 [Cucumis melo var. makuwa] >TYK10310.1 glucan endo-1,3-beta-glucosidase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 624.4 bits (1609), Expect = 6.2e-175
Identity = 339/362 (93.65%), Postives = 345/362 (95.30%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQ ASKCFFFSI+SLLAICSSGRFGGSHL VEEN S ELKWKF E GRTTMHDATTNFP
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKWKFIEFGRTTMHDATTNFP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TTPDTSTPTIITVPSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           GAQPITNPVTTYP PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQ
Sbjct: 121 GAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+G+GMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGSGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPG FNTDNPASSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIIT
Sbjct: 301 GSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGLRPFTCFIILTMSFITHRIIT 360

Query: 361 LD 363
           LD
Sbjct: 361 LD 362

BLAST of Lsi06G000270 vs. NCBI nr
Match: XP_038879242.1 (mucin-2 isoform X2 [Benincasa hispida])

HSP 1 Score: 621.3 bits (1601), Expect = 5.2e-174
Identity = 341/363 (93.94%), Postives = 348/363 (95.87%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFP 60
           MAQTASKCFFFSILSLLAICSSGR   SHL VEEN STEL+WKFTELGRTT+HDATTN P
Sbjct: 1   MAQTASKCFFFSILSLLAICSSGR---SHLVVEENGSTELQWKFTELGRTTIHDATTNSP 60

Query: 61  TTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120
           TT DTSTPTIITVPSTNPVTVTPSSP ATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP
Sbjct: 61  TTLDTSTPTIITVPSTNPVTVTPSSPVATPVSIPLTTPITVPANSPVPLTNPVAPPVTVP 120

Query: 121 GAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQ 180
           G QPITNPVTTYP P+GGAP LTPATNPVPVSPPATTNAP+IPGQSWCVARSGAS+MALQ
Sbjct: 121 GVQPITNPVTTYPAPTGGAPGLTPATNPVPVSPPATTNAPVIPGQSWCVARSGASQMALQ 180

Query: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240
           SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN
Sbjct: 181 SALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTN 240

Query: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPEN 300
           SNPSTGSCIYPSSSSSATPASMTPSVPTPT TTTAPITVSPTTVTNPTTSSP+G GMPEN
Sbjct: 241 SNPSTGSCIYPSSSSSATPASMTPSVPTPTPTTTAPITVSPTTVTNPTTSSPLGNGMPEN 300

Query: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIIT 360
           GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPF CCIILT+SLITR IIT
Sbjct: 301 GSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFTCCIILTISLITRLIIT 360

Query: 361 LDW 364
           LDW
Sbjct: 361 LDW 360

BLAST of Lsi06G000270 vs. NCBI nr
Match: KGN66453.2 (hypothetical protein Csa_007420 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 2.7e-170
Identity = 330/350 (94.29%), Postives = 336/350 (96.00%), Query Frame = 0

Query: 14  LSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDATTNFPTTPDTSTPTIITV 73
           +SLLAICSSGRFGGSHL VEENAS+ELKWKF E GRTTMHDATTNFPTTPDTSTPTIITV
Sbjct: 1   MSLLAICSSGRFGGSHLGVEENASSELKWKFPEFGRTTMHDATTNFPTTPDTSTPTIITV 60

Query: 74  PSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYP 133
           PSTNPVT+TPSSPAATPVSIPLTTP TVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYP
Sbjct: 61  PSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYP 120

Query: 134 PPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASEMALQSALDYACGTGAAD 193
            PSGGAPVLTP TNPVPVSPPATTNAP+IPGQSWCVARSGASEMALQSALDYACGTG AD
Sbjct: 121 APSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVARSGASEMALQSALDYACGTGGAD 180

Query: 194 CSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSS 253
           CSQIQQ GSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSS
Sbjct: 181 CSQIQQSGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSS 240

Query: 254 SSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPENGSPPGVFNTDNPA 313
           SSSATPASMTPSVPT T TTTAPITVSPTTVTNPTTSSP+GTGMPENGSPPGVFNTDNPA
Sbjct: 241 SSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTSSPVGTGMPENGSPPGVFNTDNPA 300

Query: 314 SSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITRRIITLDW 364
           SSIGSTTGFGTEIPPSSSTSIS+AAGLRPF C IILTMS IT RIITLDW
Sbjct: 301 SSIGSTTGFGTEIPPSSSTSISVAAGLRPFTCFIILTMSFITHRIITLDW 350

BLAST of Lsi06G000270 vs. NCBI nr
Match: XP_022961450.1 (mucin-2 [Cucurbita moschata] >KAG6590512.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 594.7 bits (1532), Expect = 5.2e-166
Identity = 327/362 (90.33%), Postives = 344/362 (95.03%), Query Frame = 0

Query: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDA----T 60
           MAQTASKCFFFS+LSLLAICSSGRFGGSHL+V+ENAS EL+WKF+EL RTTMHDA    T
Sbjct: 1   MAQTASKCFFFSVLSLLAICSSGRFGGSHLDVQENASAELEWKFSELVRTTMHDATDSPT 60

Query: 61  TNFPTTPDTSTPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPP 120
           TNFPTTPDTSTPTIITVP+TNP+TVTPSSPA+TPVSIPLTTPITVPANSPVPLTNPVAPP
Sbjct: 61  TNFPTTPDTSTPTIITVPATNPITVTPSSPASTPVSIPLTTPITVPANSPVPLTNPVAPP 120

Query: 121 VTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPATTNAPIIPGQSWCVARSGASE 180
           VTVPGAQPITNPVTTYP PSGG+PV+TP TNPVPVSPPA TNAP+IPGQSWCVARSGASE
Sbjct: 121 VTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVARSGASE 180

Query: 181 MALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSA 240
           MALQSALDYACGTG ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPS TSCDFGGSA
Sbjct: 181 MALQSALDYACGTG-ADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSCDFGGSA 240

Query: 241 MVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTG 300
           MVTNSNPSTGSC+YPSSSSSATPASMTPSVPTPT TTTAPITV+PT VTNP TSSP+GTG
Sbjct: 241 MVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TSSPVGTG 300

Query: 301 MPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISMAAGLRPFPCCIILTMSLITR 359
           MPENG+PP VFN DNPASSIGS+TGFGTEIPPSSSTSISMAAGLRPF  CII+TMSLITR
Sbjct: 301 MPENGTPPAVFNPDNPASSIGSSTGFGTEIPPSSSTSISMAAGLRPFTWCIIMTMSLITR 360

BLAST of Lsi06G000270 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.5e-49
Identity = 153/340 (45.00%), Postives = 197/340 (57.94%), Query Frame = 0

Query: 9   FFFSILSLLAICSSGRFGGSHLEVEENASTELKWKFTELGRTTMHDA---TTNFPTTPDT 68
           F F  LSLL+ CSS                           TT HD     T FPT P T
Sbjct: 10  FTFLFLSLLSYCSS--------------------------TTTHHDVLNPPTVFPTNP-T 69

Query: 69  STPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVPLTNPVAPPVTVPGAQPI 128
           +TPT  T P   PVT+TP++PA T   +P  T I  P  +P P+     P +T P   P+
Sbjct: 70  TTPT-ATFP---PVTITPTNPATTVPIVPPVTTIPPPTLTPPPVITIPPPTLTPPVTNPV 129

Query: 129 TNPVTTYPP--PSGGAPVLTPATNPVP-VSPPATTNAPIIPGQSWCVARSGASEMALQSA 188
           TNPVT YPP  PSG  PV      PVP V+PP  +N+P + GQSWCVA+ GAS+++LQ A
Sbjct: 130 TNPVTQYPPTQPSGTVPV------PVPVVAPPVVSNSPSVSGQSWCVAKPGASQVSLQQA 189

Query: 189 LDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSN 248
           LDYAC  G ADCSQ+QQGG+CY+P +L++HASFAFNSY+QKNPS  SCDFGG+A + N+N
Sbjct: 190 LDYAC--GIADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSPQSCDFGGAASLVNTN 249

Query: 249 PSTGSCIYPSSSSSATPASMTPSVPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPENGS 308
           PSTGSCIY + SS++TP +   + PTP++ T     V+ T +       P G G+   G+
Sbjct: 250 PSTGSCIYQTGSSTSTPMTAGTTTPTPSTQTVNQPPVTSTPII------PTGGGIIGVGT 304

Query: 309 PPGVFNTDNPA-------SSIGSTTGFGTEIPPSSSTSIS 336
           PP +FN  NP        SS G   G+G +  P+ +   S
Sbjct: 310 PPAIFNPANPTSNTLNNPSSGGLAGGYGFDGSPNENNPTS 304

BLAST of Lsi06G000270 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.2e-27
Identity = 88/200 (44.00%), Postives = 121/200 (60.50%), Query Frame = 0

Query: 96  TTPITVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPA 155
           T  IT P      +T P+A         P T P T  P     A  +  AT P+ +  P+
Sbjct: 21  TKAITEPIEEEKDITTPLA-------TNPTTTPTTVVPNSDSDASAV--ATTPLTI--PS 80

Query: 156 TTNAPIIPG-QSWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHAS 215
           + +    PG QSWCVAR   ++MALQ+ALDYACG G ADCS+IQ+GG+CYNPN+L  HAS
Sbjct: 81  SPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHAS 140

Query: 216 FAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMT----------- 275
           FAFNSY+QKNP  +SC+F G+A+  +++PS GSC +PS+S+S +  ++T           
Sbjct: 141 FAFNSYYQKNPIPSSCNFDGTAITISADPSLGSCHFPSTSTSESILNVTSEDGLGLFGRI 200

Query: 276 PSVPTP---TSTTTAPITVS 281
           PS PTP    ST+++   +S
Sbjct: 201 PSHPTPKPEASTSSSRTLIS 209

BLAST of Lsi06G000270 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 2.3e-26
Identity = 72/150 (48.00%), Postives = 95/150 (63.33%), Query Frame = 0

Query: 96  TTPITVPANSPVPLTNPVAPPVTVPGAQPITNPVTTYPPPSGGAPVLTPATNPVPVSPPA 155
           T  IT P      +T P+A         P T P T  P     A  +  AT P+ +  P+
Sbjct: 21  TKAITEPIEEEKDITTPLA-------TNPTTTPTTVVPNSDSDASAV--ATTPLTI--PS 80

Query: 156 TTNAPIIPG-QSWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHAS 215
           + +    PG QSWCVAR   ++MALQ+ALDYACG G ADCS+IQ+GG+CYNPN+L  HAS
Sbjct: 81  SPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHAS 140

Query: 216 FAFNSYFQKNPSSTSCDFGGSAMVTNSNPS 245
           FAFNSY+QKNP  +SC+F G+A+  +++PS
Sbjct: 141 FAFNSYYQKNPIPSSCNFDGTAITISADPS 159

BLAST of Lsi06G000270 vs. TAIR 10
Match: AT1G69295.1 (plasmodesmata callose-binding protein 4 )

HSP 1 Score: 103.6 bits (257), Expect = 3.4e-22
Identity = 78/174 (44.83%), Postives = 106/174 (60.92%), Query Frame = 0

Query: 166 SWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNP 225
           ++C+ + G +E  LQ A+DYACG G ADC+QIQ  G+CY PNT++NH   A NSY+QK  
Sbjct: 20  AYCLCKEG-NEQVLQKAIDYACGNG-ADCTQIQPTGACYQPNTVKNHCDVAVNSYYQKKA 79

Query: 226 SS-TSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTPTS---TTTAPITVSP 285
           SS  +CDF G+A  + + PST S     SSSS TP + TP+  TPTS   TT  P T +P
Sbjct: 80  SSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTSGTPTTGTPTTGTP 139

Query: 286 TTVTNPTTSSPI----GTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSS 332
           TT T PT+ +P      TG P  G+  G+ N++   +S  S+   GT + P+ S
Sbjct: 140 TTGT-PTSGTPTSGFPNTGTPNTGTNTGMPNSNGMPTSSSSSVFPGTTLGPTGS 190

BLAST of Lsi06G000270 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-20
Identity = 108/310 (34.84%), Postives = 148/310 (47.74%), Query Frame = 0

Query: 66  STPTIITVPSTNPVTVTPSSPAATPVSIPLTTPITVPANSPVP-LTNPVAPPVTVPGAQP 125
           ST  ++ +  T+P+ V           IP+  P     ++  P +T P  P +T PG   
Sbjct: 11  STIMLLCLLYTSPLDVIAQGQGGQG-DIPVVNPTAPGGSTTTPTITQPSPPSLTFPGP-- 70

Query: 126 ITNPVTTYPP-----PSGGAPVLTPATNP---------------VPVSPPA--------- 185
            T P   YPP     P+GG P L   T P                P              
Sbjct: 71  -TTPTGGYPPLDGTTPTGGYPPLYGTTPPGGGDVGGGGGGYGGGTPGGGGGGGGDTGAGA 130

Query: 186 ----------TTNAPIIPGQSWCVARSGASEMALQSALDYACGTGAADCSQIQQGGSCYN 245
                     T     +    WC+A++ AS  +LQ ALDYACG G ADC QIQQG +CY 
Sbjct: 131 GGGGYGGGGDTGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYE 190

Query: 246 PNTLENHASFAFNSYFQKNPSSTSCDFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPS 305
           PNT+ +HASFAFNSY+QK+P S SC+FGG+A +T+++PS GSC +  SSSS T ++  PS
Sbjct: 191 PNTIRDHASFAFNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHF--SSSSGTVSTSPPS 250

Query: 306 VPTPTSTTTAPITVSPTTVTNPTTSSPIGTGMPENGSPPGVFNTDNPASSIGSTTGFGTE 336
             +P   ++ P +  P  +T PT      TG+  +G P GV             TG    
Sbjct: 251 QMSPPDFSSPPSSTYPPPITTPT------TGITGSGPPFGV----------AEPTGL--- 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93V724.8e-2144.83PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4... [more]
Q9FZ861.1e-2049.18PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3... [more]
O653991.4e-2052.88Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9FZD07.2e-1740.31Carbohydrate-binding X8 domain-containing protein OS=Arabidopsis thaliana OX=370... [more]
Q9FNQ27.2e-1736.84PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1... [more]
Match NameE-valueIdentityDescription
A0A0A0M1B64.9e-17894.49Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A5A7U6N83.0e-17593.65Glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BQE93.0e-17593.65glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo OX=3656 GN=LOC103492098 PE=4 ... [more]
A0A6J1HC942.5e-16690.33mucin-2 OS=Cucurbita moschata OX=3662 GN=LOC111462029 PE=4 SV=1[more]
A0A6J1HUD25.6e-16690.08mucin-2 OS=Cucurbita maxima OX=3661 GN=LOC111467890 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135574.11.0e-17794.49mucin-5AC [Cucumis sativus][more]
XP_008450517.16.2e-17593.65PREDICTED: glucan endo-1,3-beta-glucosidase 4 [Cucumis melo] >KAA0050964.1 gluca... [more]
XP_038879242.15.2e-17493.94mucin-2 isoform X2 [Benincasa hispida][more]
KGN66453.22.7e-17094.29hypothetical protein Csa_007420 [Cucumis sativus][more]
XP_022961450.15.2e-16690.33mucin-2 [Cucurbita moschata] >KAG6590512.1 PLASMODESMATA CALLOSE-BINDING PROTEIN... [more]
Match NameE-valueIdentityDescription
AT1G09460.19.5e-4945.00Carbohydrate-binding X8 domain superfamily protein [more]
AT2G30933.11.2e-2744.00Carbohydrate-binding X8 domain superfamily protein [more]
AT2G30933.22.3e-2648.00Carbohydrate-binding X8 domain superfamily protein [more]
AT1G69295.13.4e-2244.83plasmodesmata callose-binding protein 4 [more]
AT1G29380.13.2e-2034.84Carbohydrate-binding X8 domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01217PRICHEXTENSNcoord: 78..94
score: 40.0
coord: 108..129
score: 34.55
coord: 260..285
score: 33.08
coord: 62..74
score: 44.62
coord: 133..149
score: 35.29
NoneNo IPR availableGENE3D1.20.58.1040coord: 162..263
e-value: 5.3E-12
score: 48.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..331
NoneNo IPR availablePANTHERPTHR32227:SF344CARBOHYDRATE-BINDING X8 DOMAIN PROTEINcoord: 47..325
IPR012946X8 domainSMARTSM00768X8_clscoord: 166..250
e-value: 9.0E-48
score: 174.7
IPR012946X8 domainPFAMPF07983X8coord: 167..237
e-value: 2.4E-21
score: 76.2
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 47..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G000270.1Lsi06G000270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0009506 plasmodesma
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds