Lsi05G015230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G015230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMitochondrial transcription termination factor family protein
Locationchr05: 23030608 .. 23031705 (-)
RNA-Seq ExpressionLsi05G015230
SyntenyLsi05G015230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATCATTTGCTTCGTAGAATCGTTCTAGTTAGACCTCCATCATCAGTCTCTGCTCACGGATTTTCTGAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGATTGTATCATCTCCGAAATCTGCTTCGTTGGCTTCTAATTTTGTTCAGGTTAAGAACAATGAGGAGCCTTTAATTGTGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCCGAGCTCGTCAAGAAGTTCCCTCAGGTTCTTTCAGCGAACCTCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGGTTTTCCTTTCGTTTTAAAACGAAGTTTAAACAGACAAATTATTCCAACCTTTGATTACATTCAAGCCCTGCTTCAAACAGAGGAGAAGACTATCGCAGTGATAAAACGCTTCGTGGGTATTCTCACTTGGGATCTTCGAATTTCAGCTGGTCCCAATATTGAAATTCTGAAACAAATTGGAGTGCCTGATTCCAATATTTCAACCATTCTTCGGTACCAGCCTCGAGTGTTCTTGACAAATTCCATCCGATTCAAGGAGATTGTAGAGGAAGTTAAGGAAATGGGATTCAATCCTCAGCGATTGAAGTTTGTTCTTGCTGTTTTTGCTCTGCGAACAATGAGCAAATCTACCTGGGATAAGAAGGTTGAAGTTTATAGGAAATGGGGATGGTCTGAAGAAGAGATCCGTTTGGCTTTTGGTAGGCATCCATGGTGTATGATGGCTTCTGAGGATAAGATTAATGGTGTAATGGATTATTTTGTCAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACATAGCTTAAAGAAGAGGATTTTGCCTAGAGGCTCTGTTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAACATGGGAATCTTCCTTTGTTGTTTGATTCTACTGAAAATCGCTTCTTAGACAAATTCATCAACCCTTATAAGGAGCGGATTCCTGGATTGTTGAAGTTTTATACAGAGAAATTAAAGGATTCTAAAAGATGA

mRNA sequence

ATGTATCATTTGCTTCGTAGAATCGTTCTAGTTAGACCTCCATCATCAGTCTCTGCTCACGGATTTTCTGAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGATTGTATCATCTCCGAAATCTGCTTCGTTGGCTTCTAATTTTGTTCAGGTTAAGAACAATGAGGAGCCTTTAATTGTGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCCGAGCTCGTCAAGAAGTTCCCTCAGGTTCTTTCAGCGAACCTCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGGTTTTCCTTTCGTTTTAAAACGAAGTTTAAACAGACAAATTATTCCAACCTTTGATTACATTCAAGCCCTGCTTCAAACAGAGGAGAAGACTATCGCAGTGATAAAACGCTTCGTGGGTATTCTCACTTGGGATCTTCGAATTTCAGCTGGTCCCAATATTGAAATTCTGAAACAAATTGGAGTGCCTGATTCCAATATTTCAACCATTCTTCGGTACCAGCCTCGAGTGTTCTTGACAAATTCCATCCGATTCAAGGAGATTGTAGAGGAAGTTAAGGAAATGGGATTCAATCCTCAGCGATTGAAGTTTGTTCTTGCTGTTTTTGCTCTGCGAACAATGAGCAAATCTACCTGGGATAAGAAGGTTGAAGTTTATAGGAAATGGGGATGGTCTGAAGAAGAGATCCGTTTGGCTTTTGGTAGGCATCCATGGTGTATGATGGCTTCTGAGGATAAGATTAATGGTGTAATGGATTATTTTGTCAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACATAGCTTAAAGAAGAGGATTTTGCCTAGAGGCTCTGTTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAACATGGGAATCTTCCTTTGTTGTTTGATTCTACTGAAAATCGCTTCTTAGACAAATTCATCAACCCTTATAAGGAGCGGATTCCTGGATTGTTGAAGTTTTATACAGAGAAATTAAAGGATTCTAAAAGATGA

Coding sequence (CDS)

ATGTATCATTTGCTTCGTAGAATCGTTCTAGTTAGACCTCCATCATCAGTCTCTGCTCACGGATTTTCTGAAAGTCCGTTGAAATCTCTCAGATACTTATCGACCTCTTCTGAGATTGTATCATCTCCGAAATCTGCTTCGTTGGCTTCTAATTTTGTTCAGGTTAAGAACAATGAGGAGCCTTTAATTGTGTTCTTTGAGAATCATGGATTCTCTAAATCACAGATCTCCGAGCTCGTCAAGAAGTTCCCTCAGGTTCTTTCAGCGAACCTCGAGAAAACCCTATTGCCCAAATTGTTGTTCTTTCAATCTAAAGGCCTTTCTTCCCCCGAGATTGCCAAATTAATATGTGGTTTTCCTTTCGTTTTAAAACGAAGTTTAAACAGACAAATTATTCCAACCTTTGATTACATTCAAGCCCTGCTTCAAACAGAGGAGAAGACTATCGCAGTGATAAAACGCTTCGTGGGTATTCTCACTTGGGATCTTCGAATTTCAGCTGGTCCCAATATTGAAATTCTGAAACAAATTGGAGTGCCTGATTCCAATATTTCAACCATTCTTCGGTACCAGCCTCGAGTGTTCTTGACAAATTCCATCCGATTCAAGGAGATTGTAGAGGAAGTTAAGGAAATGGGATTCAATCCTCAGCGATTGAAGTTTGTTCTTGCTGTTTTTGCTCTGCGAACAATGAGCAAATCTACCTGGGATAAGAAGGTTGAAGTTTATAGGAAATGGGGATGGTCTGAAGAAGAGATCCGTTTGGCTTTTGGTAGGCATCCATGGTGTATGATGGCTTCTGAGGATAAGATTAATGGTGTAATGGATTATTTTGTCAACAAGATTGGATGTGAATCTTCTTTCATTGCTAGGAGACCTGCTCTAATTTCACATAGCTTAAAGAAGAGGATTTTGCCTAGAGGCTCTGTTTATCAAGTTTTGCTGTCAAAGGGTTTGATTAAGAAACATGGGAATCTTCCTTTGTTGTTTGATTCTACTGAAAATCGCTTCTTAGACAAATTCATCAACCCTTATAAGGAGCGGATTCCTGGATTGTTGAAGTTTTATACAGAGAAATTAAAGGATTCTAAAAGATGA

Protein sequence

MYHLLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDSKR
Homology
[Close]
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-09
Identity = 51/265 (19.25%), Postives = 123/265 (46.42%), Query Frame = 0 

Query: 61  PLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFP 120
           P++ + +      S +  +++++P+VL   LE T+   + +    G++  EI  ++  +P
Sbjct: 217 PVVKYLQGLDIKPSDVPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYP 276

Query: 121 FVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVP 180
            +L   + R I P  +Y++ L         +I++   IL ++L  +  PN++IL+   V 
Sbjct: 277 EILGMRVARIIKPLVEYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVR 336

Query: 181 DSNISTILRYQPRVFLTNSIRFKEIVEEVKEM-----GFNPQRL-KFVLAVFALRTMSKS 240
           ++++ +I+   P +     I  K  ++  +++       NP+ L   +  +    ++S+S
Sbjct: 337 ETSLPSIIAQYPEII---GIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSES 396

Query: 241 TWDKKVEVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPA 300
              K ++   K G+S ++ R      P  +  +   +    +YF  ++      +   PA
Sbjct: 397 PMLKHIDFLTKCGFSIDQTREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPA 456

Query: 301 LISHSLKKRILPRGSVYQVLLSKGL 320
             ++ L+  + PR   ++ ++ KG+
Sbjct: 457 FFTYGLESTVKPR---HKKIIKKGI 475

[Close]
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-08
Identity = 36/138 (26.09%), Postives = 78/138 (56.52%), Query Frame = 0 

Query: 61  PLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSP-EIAKLICGF 120
           PL+ FF+  G  ++Q+ +++   P+++S +++  L   + F  S GL     I K++   
Sbjct: 116 PLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLVKN 175

Query: 121 PFVLKRSLNRQIIPTFDYIQALLQTEEKTI-AVIKRFVGILTWDLRISAGPNIEILKQIG 180
           PF++  S+++++ PT +++++ +   E  I +V+  F  +L  D+     PN + LK+ G
Sbjct: 176 PFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKECG 235

Query: 181 VPDSNISTILRYQPRVFL 197
             DS I+T++   P++ +
Sbjct: 236 FGDSQIATMVTGYPQILI 253

[Close]
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 5.2e-07
Identity = 49/263 (18.63%), Postives = 117/263 (44.49%), Query Frame = 0 

Query: 61  PLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFP 120
           P++ + +      + +  +++++P++L   LE T+   + +    G+   ++  +I  FP
Sbjct: 173 PVVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFP 232

Query: 121 FVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVP 180
            VL   + + I P  ++++ +         +I++   +L + L+    PNIE L  IGV 
Sbjct: 233 EVLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVR 292

Query: 181 DSNISTILRYQPRVFLTNSIRFKEIVEE-VKEMGFNPQRLKFVLAVFALR---TMSKSTW 240
              +++I+   P V L   +R K + ++ + E      R  F   +  +    ++ ++  
Sbjct: 293 KEALASIVMQYPDV-LGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAV 352

Query: 241 DKKVEVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALI 300
            K V      G+   ++       P  +  + D +    +YF N++  +   +   PA  
Sbjct: 353 LKHVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFF 412

Query: 301 SHSLKKRILPRGSVYQVLLSKGL 320
           ++ ++  + PR   ++++  KGL
Sbjct: 413 TYGIESTVRPR---HEMVSRKGL 431

[Close]
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.7e-06
Identity = 38/173 (21.97%), Postives = 82/173 (47.40%), Query Frame = 0 

Query: 55  VKNNEEPLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAK 114
           V+ N  P++ +    G  +  + +L++++PQVL A++   L P + + Q   +   ++ +
Sbjct: 140 VRKNMVPVLDYLGKLGVRQDALPDLLRRYPQVLHASVVVDLAPVVKYLQGMDVRPHDVPR 199

Query: 115 LICGFPFVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEIL 174
           ++  +P +L   L   +  +  Y+  +     +  +VI RF  +L   +     P +E L
Sbjct: 200 VLERYPELLGFKLEGTMSTSIAYLVGIGVARRQVGSVITRFPEVLGMRVGKIIKPFVEHL 259

Query: 175 KQIGVPDSNISTILRYQPRVF-LTNSIRFKEIVEEVKEMGFNPQRLKFVLAVF 227
           + IG+    I+ I+  +P V       + K  +E + E G   + L F++A +
Sbjct: 260 EGIGLQRLAIARIIEKKPYVLGFGLEDKVKPNIEALLEFGVRKEALAFIVAQY 312

[Close]
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 8.3e-05
Identity = 31/126 (24.60%), Postives = 58/126 (46.03%), Query Frame = 0 

Query: 55  VKNNEEPLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAK 114
           ++   +PL+ +F   G  K  +  ++   P +   +LEKT+ PK+ F Q  G+ +  I  
Sbjct: 384 IEERWKPLVKYFYYLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGN 443

Query: 115 LICGFPFVLKRSLNRQIIPTFDYIQALLQTEEKTIA-VIKRFVGILTWDLRISAGPNIEI 174
           ++  FP +L  SL ++I P   ++       +K I  VI     +L   +     PN+  
Sbjct: 444 MLVKFPSLLTNSLYKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRY 503

Query: 175 LKQIGV 180
              +G+
Sbjct: 504 YISLGI 509

[Close]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match: A0A6J1EZH0 (uncharacterized protein LOC111440613 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440613 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.5e-163
Identity = 297/362 (82.04%), Postives = 324/362 (89.50%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI +VR PSSV AHGFSESPLKSLRYLSTSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 97  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 156

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 157 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 216

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 217 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 276

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 277 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 336

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 337 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 396

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 397 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 456

Query: 364 KR 366
           KR
Sbjct: 457 KR 458

[Close]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match: A0A6J1F4G8 (uncharacterized protein LOC111440613 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440613 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.5e-163
Identity = 297/362 (82.04%), Postives = 324/362 (89.50%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI +VR PSSV AHGFSESPLKSLRYLSTSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 98  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 157

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 158 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 217

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 218 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 277

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 278 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 337

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 338 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 397

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 398 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 457

Query: 364 KR 366
           KR
Sbjct: 458 KR 459

[Close]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match: A0A6J1I9L4 (transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111472348 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.0e-162
Identity = 296/365 (81.10%), Postives = 327/365 (89.59%), Query Frame = 0 

Query: 1   MYHLLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEE 60
           MYHLLRRI+LVR P SV +  FS SPLKSLR+L TSSEIVSSPKSASLASNFV  ++NE+
Sbjct: 1   MYHLLRRIILVRTPLSVES--FSGSPLKSLRFLLTSSEIVSSPKSASLASNFVDFESNEK 60

Query: 61  PLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFP 120
           P+I+FFENHGFSKSQISELVKKFPQVLS N EKTLLPKLLF QSKGLSSPE+AKL+CGFP
Sbjct: 61  PVILFFENHGFSKSQISELVKKFPQVLSTNPEKTLLPKLLFLQSKGLSSPEVAKLVCGFP 120

Query: 121 FVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVP 180
            +LKRSLNRQIIP+F+YI  LLQTEEKTIA IKRF G+LTWDL+ SA PN+EIL+QIGVP
Sbjct: 121 GILKRSLNRQIIPSFNYIHDLLQTEEKTIAAIKRFGGLLTWDLQNSARPNVEILRQIGVP 180

Query: 181 DSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKV 240
           DSNI++ILR QPRVFL + IRF++IVEEVKEMGFNP RLKFVLAVFALR MSKSTW KKV
Sbjct: 181 DSNIASILRSQPRVFLISPIRFRKIVEEVKEMGFNPLRLKFVLAVFALRAMSKSTWKKKV 240

Query: 241 EVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSL 300
           EVYRKWGW EEEI  AFGRHPWCMMAS DKINGVMD+FVN+IGCESS+IAR P+LISHSL
Sbjct: 241 EVYRKWGWPEEEILFAFGRHPWCMMASADKINGVMDFFVNQIGCESSYIARIPSLISHSL 300

Query: 301 KKRILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKL 360
           KKRI+PRGSVYQVLLS GLIKKH NLPLLF STE RFLDKFI P+K+RIPGLLK YTEKL
Sbjct: 301 KKRIVPRGSVYQVLLSNGLIKKHVNLPLLFVSTEKRFLDKFIEPHKKRIPGLLKLYTEKL 360

Query: 361 KDSKR 366
           + S +
Sbjct: 361 EVSSK 363

[Close]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match: A0A6J1GVP2 (uncharacterized protein LOC111457902 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457902 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-161
Identity = 293/362 (80.94%), Postives = 321/362 (88.67%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI++V  PSSV  HGFSESPLKSLRY STSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 3   LLRRIIVVSRPSSVFVHGFSESPLKSLRYSSTSSEIASSPKSASPDSNSVEPENDEKPVI 62

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           V FENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 63  VLFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 123 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 183 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 243 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 302

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 362

Query: 364 KR 366
           KR
Sbjct: 363 KR 364

[Close]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match: A0A6J1IXF7 (uncharacterized protein LOC111479414 OS=Cucurbita maxima OX=3661 GN=LOC111479414 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.7e-160
Identity = 291/362 (80.39%), Postives = 322/362 (88.95%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI++VR PSSV AHGFSESP KSLRY STSS I SS KSAS  SN V+ +NNE+P+I
Sbjct: 3   LLRRIIVVRQPSSVFAHGFSESPFKSLRYSSTSSGIASSLKSASPDSNSVKPENNEKPVI 62

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 63  VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQALLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 123 TRSLDRKIIPAFDYIQALLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL+YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 183 IATILQYQPRVFLINNVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNK+GCE S+IA+RPALIS SLKKR
Sbjct: 243 KKWGWPEEEILFAFRRHPWCMMASEDKINGVMDFFVNKLGCEPSYIAKRPALISLSLKKR 302

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+K+ IPGLLK Y +KL D+
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKKLIPGLLKLYKKKLVDA 362

Query: 364 KR 366
           KR
Sbjct: 363 KR 364

[Close]
BLAST of Lsi05G015230 vs. NCBI nr
Match: XP_022933339.1 (uncharacterized protein LOC111440613 isoform X2 [Cucurbita moschata])

HSP 1 Score: 583.9 bits (1504), Expect = 9.3e-163
Identity = 297/362 (82.04%), Postives = 324/362 (89.50%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI +VR PSSV AHGFSESPLKSLRYLSTSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 97  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 156

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 157 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 216

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 217 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 276

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 277 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 336

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 337 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 396

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 397 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 456

Query: 364 KR 366
           KR
Sbjct: 457 KR 458

[Close]
BLAST of Lsi05G015230 vs. NCBI nr
Match: XP_022933338.1 (uncharacterized protein LOC111440613 isoform X1 [Cucurbita moschata])

HSP 1 Score: 583.9 bits (1504), Expect = 9.3e-163
Identity = 297/362 (82.04%), Postives = 324/362 (89.50%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI +VR PSSV AHGFSESPLKSLRYLSTSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 98  LLRRINVVRQPSSVFAHGFSESPLKSLRYLSTSSEIASSPKSASPDSNKVKPENDEKPVI 157

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 158 VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 217

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 218 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 277

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 278 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 337

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 338 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 397

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 398 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 457

Query: 364 KR 366
           KR
Sbjct: 458 KR 459

[Close]
BLAST of Lsi05G015230 vs. NCBI nr
Match: XP_022973791.1 (transcription termination factor MTERF5, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 582.8 bits (1501), Expect = 2.1e-162
Identity = 296/365 (81.10%), Postives = 327/365 (89.59%), Query Frame = 0 

Query: 1   MYHLLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEE 60
           MYHLLRRI+LVR P SV +  FS SPLKSLR+L TSSEIVSSPKSASLASNFV  ++NE+
Sbjct: 1   MYHLLRRIILVRTPLSVES--FSGSPLKSLRFLLTSSEIVSSPKSASLASNFVDFESNEK 60

Query: 61  PLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFP 120
           P+I+FFENHGFSKSQISELVKKFPQVLS N EKTLLPKLLF QSKGLSSPE+AKL+CGFP
Sbjct: 61  PVILFFENHGFSKSQISELVKKFPQVLSTNPEKTLLPKLLFLQSKGLSSPEVAKLVCGFP 120

Query: 121 FVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVP 180
            +LKRSLNRQIIP+F+YI  LLQTEEKTIA IKRF G+LTWDL+ SA PN+EIL+QIGVP
Sbjct: 121 GILKRSLNRQIIPSFNYIHDLLQTEEKTIAAIKRFGGLLTWDLQNSARPNVEILRQIGVP 180

Query: 181 DSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKV 240
           DSNI++ILR QPRVFL + IRF++IVEEVKEMGFNP RLKFVLAVFALR MSKSTW KKV
Sbjct: 181 DSNIASILRSQPRVFLISPIRFRKIVEEVKEMGFNPLRLKFVLAVFALRAMSKSTWKKKV 240

Query: 241 EVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSL 300
           EVYRKWGW EEEI  AFGRHPWCMMAS DKINGVMD+FVN+IGCESS+IAR P+LISHSL
Sbjct: 241 EVYRKWGWPEEEILFAFGRHPWCMMASADKINGVMDFFVNQIGCESSYIARIPSLISHSL 300

Query: 301 KKRILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKL 360
           KKRI+PRGSVYQVLLS GLIKKH NLPLLF STE RFLDKFI P+K+RIPGLLK YTEKL
Sbjct: 301 KKRIVPRGSVYQVLLSNGLIKKHVNLPLLFVSTEKRFLDKFIEPHKKRIPGLLKLYTEKL 360

Query: 361 KDSKR 366
           + S +
Sbjct: 361 EVSSK 363

[Close]
BLAST of Lsi05G015230 vs. NCBI nr
Match: XP_022956116.1 (uncharacterized protein LOC111457902 isoform X2 [Cucurbita moschata])

HSP 1 Score: 578.2 bits (1489), Expect = 5.1e-161
Identity = 293/362 (80.94%), Postives = 321/362 (88.67%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI++V  PSSV  HGFSESPLKSLRY STSSEI SSPKSAS  SN V+ +N+E+P+I
Sbjct: 3   LLRRIIVVSRPSSVFVHGFSESPLKSLRYSSTSSEIASSPKSASPDSNSVEPENDEKPVI 62

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           V FENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 63  VLFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQ LLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 123 MRSLDRKIIPAFDYIQELLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 183 IATILEYQPRVFLVNTVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNKIGCE S+IA+RPALIS SLKKR
Sbjct: 243 KKWGWPEEEILSAFRRHPWCMMASEDKINGVMDFFVNKIGCEPSYIAKRPALISLSLKKR 302

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+KE IPGLLK Y EKL+DS
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKELIPGLLKLYKEKLEDS 362

Query: 364 KR 366
           KR
Sbjct: 363 KR 364

[Close]
BLAST of Lsi05G015230 vs. NCBI nr
Match: XP_022979833.1 (uncharacterized protein LOC111479414 [Cucurbita maxima])

HSP 1 Score: 574.7 bits (1480), Expect = 5.6e-160
Identity = 291/362 (80.39%), Postives = 322/362 (88.95%), Query Frame = 0 

Query: 4   LLRRIVLVRPPSSVSAHGFSESPLKSLRYLSTSSEIVSSPKSASLASNFVQVKNNEEPLI 63
           LLRRI++VR PSSV AHGFSESP KSLRY STSS I SS KSAS  SN V+ +NNE+P+I
Sbjct: 3   LLRRIIVVRQPSSVFAHGFSESPFKSLRYSSTSSGIASSLKSASPDSNSVKPENNEKPVI 62

Query: 64  VFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVL 123
           VFFENHGFSK+QISELVKKFPQVLSAN EKTLLPKLLFFQSKGLSSPEIAKL+CGFP +L
Sbjct: 63  VFFENHGFSKTQISELVKKFPQVLSANPEKTLLPKLLFFQSKGLSSPEIAKLVCGFPSIL 122

Query: 124 KRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSN 183
            RSL+R+IIP FDYIQALLQTEEKTIA IKRF+GILTWDL+I A PN+E L QIGVPDS 
Sbjct: 123 TRSLDRKIIPAFDYIQALLQTEEKTIASIKRFLGILTWDLQIYARPNVETLIQIGVPDSK 182

Query: 184 ISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSKSTWDKKVEVY 243
           I+TIL+YQPRVFL N++RFKEIVEEVKEMGFNP RL FVLAVFA+R MSKSTW KKVEVY
Sbjct: 183 IATILQYQPRVFLINNVRFKEIVEEVKEMGFNPLRLNFVLAVFAMRAMSKSTWRKKVEVY 242

Query: 244 RKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKR 303
           +KWGW EEEI  AF RHPWCMMASEDKINGVMD+FVNK+GCE S+IA+RPALIS SLKKR
Sbjct: 243 KKWGWPEEEILFAFRRHPWCMMASEDKINGVMDFFVNKLGCEPSYIAKRPALISLSLKKR 302

Query: 304 ILPRGSVYQVLLSKGLIKKHGNLPLLFDSTENRFLDKFINPYKERIPGLLKFYTEKLKDS 363
           I+PRGSVYQVLLSKGLIKK  NLP LF+S ENRFLDKFI+P+K+ IPGLLK Y +KL D+
Sbjct: 303 IVPRGSVYQVLLSKGLIKKDVNLPFLFESAENRFLDKFIDPHKKLIPGLLKLYKKKLVDA 362

Query: 364 KR 366
           KR
Sbjct: 363 KR 364

[Close]
BLAST of Lsi05G015230 vs. TAIR 10
Match: AT5G07900.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.8e-67
Identity = 136/331 (41.09%), Postives = 207/331 (62.54%), Query Frame = 0 

Query: 29  SLRYLSTSSEIVSSPKSASLASNFVQVKNNEEP--LIVFFENHGFSKSQISELVKKFPQV 88
           +L YL  S  +  SP SA++AS  + + + E P  ++    +HGF+ +QIS LVKK P +
Sbjct: 71  TLNYLIDSCGL--SPDSATVASRKLLLDSPERPNTVLNLLRDHGFTTAQISSLVKKRPVL 130

Query: 89  LSANLEKTLLPKLLFFQSKGLSSPEIAKLICGFPFVLKRSLNRQIIPTFDYIQALLQTEE 148
           L AN E  LLPKL FF S G+S   +A+ +   P +L RSL  Q+IP++++++++L ++E
Sbjct: 131 LLANAESVLLPKLSFFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDSDE 190

Query: 149 KTIAVIKRFVGILTWDLRISAGPNIEILKQIGVPDSNISTILRYQPRVFLTNSIRFKEIV 208
           K +A ++R   +   D   +  PNI  + + GVP+  I  +L + P   +  +  F+ I 
Sbjct: 191 KIVAALRRTTWVFLEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAIA 250

Query: 209 EEVKEMGFNPQRLKFVLAVFALRTM-SKSTWDKKVEVYRKWGWSEEEIRLAFGRHPWCMM 268
           ++ +EMGFNPQ+  FVLA+ AL    +KS WDK  EVY++WGWSE++I  AF +HP CMM
Sbjct: 251 KQAQEMGFNPQKSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCMM 310

Query: 269 ASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKGLIKKHGN 328
            SE KIN  M+YFVN++      IA+ P ++  SL+KRI+PR SV +VL+S GL+K+  +
Sbjct: 311 LSERKINRTMEYFVNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDWS 370

Query: 329 LPLLFDSTENRFLDKFINPYKERIPGLLKFY 357
           L  L    E  FL+K +  Y+E +P L+  Y
Sbjct: 371 LTSLLVPVEKVFLEKLVIKYEEELPELMNLY 399

[Close]
BLAST of Lsi05G015230 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.4e-55
Identity = 113/321 (35.20%), Postives = 198/321 (61.68%), Query Frame = 0 

Query: 42  SPKSASLASNFVQVKNNEEP--LIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKL 101
           S +SA   S FV++ ++++P  ++  F++HGF+  QI+ ++K FP+VLS + E  + PKL
Sbjct: 69  SLESAKSNSRFVKLVSSKKPDSVLALFKDHGFTNDQITSVIKSFPRVLSLSPEDVISPKL 128

Query: 102 LFFQSKGLSSPEIAKLICGFPFVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVGIL 161
           +FF S G S+ + AK+I   P +L  SL++++IP +D ++++L  EE  +  +KR  GI 
Sbjct: 129 MFFSSIGFSTSDTAKMISSSPKMLSYSLHKRLIPCYDSLKSILVEEESVVKCLKR--GIR 188

Query: 162 TWDLRIS--AGPNIEILKQIGVPDSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQ 221
            + L+I+      + I +++GVPD +I  +++  P  F +   RF E++  V   GF+P+
Sbjct: 189 CFSLKITHCVSLRVSICRELGVPDKSIKWLVQASPFTFFSRERRFNEVLNRVCSYGFDPK 248

Query: 222 RLKFVLAVFALRTMSKSTWDKKVEVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDY 281
           +  FV A+ A    S+S  ++K ++++ +GWS+E+   A  R P C+  S++KI   ++Y
Sbjct: 249 KAGFVHAMVAFDCTSESAMERKFKLFQCFGWSKEDFVAAIMRFPNCVTVSDEKIMYTLEY 308

Query: 282 FVNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKGLIKKHG-NLPLLFDSTENR 341
            VN IG ++  I  RP ++S S++KRI PR  V  +LLSKGL+KK   N   +     + 
Sbjct: 309 LVNNIGLQARDIVARPVVLSLSMEKRIKPRNQVISLLLSKGLVKKEDINYFTILKLKSSE 368

Query: 342 FLDKFINPYKERIPGLLKFYT 358
           F+DKF+  Y++ +P L++ +T
Sbjct: 369 FMDKFVLKYQDEMPQLVQPFT 387

[Close]
BLAST of Lsi05G015230 vs. TAIR 10
Match: AT5G64950.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 168.7 bits (426), Expect = 8.7e-42
Identity = 94/315 (29.84%), Postives = 183/315 (58.10%), Query Frame = 0 

Query: 43  PKSASLASNFVQVKNNEEP--LIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLL 102
           P + ++A  +  +K+ E+P  +I   +++ FS +QI + ++  P+++  N+EK L PKL 
Sbjct: 53  PLAIAIAMRYPNLKSLEQPRSVIQMLKSYSFSDTQIQKSIRVHPRMMFYNVEKILEPKLR 112

Query: 103 FFQSKGLSSPEIAKLICGFPFVLKRSLNRQIIPTFDYIQALLQTEEKTIAVIKRFVG--I 162
           FF+  G +   + K +     V+  SL +++IPT + +++++  + + + VI    G  +
Sbjct: 113 FFKDIGFTGSGLGKFVSQNSSVVGVSLVKKLIPTVEILKSIVAPKHEDLPVILSRCGWLL 172

Query: 163 LTWDLRISAGPNIEILKQIGVPDSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQR 222
           L+ D  +   PNI  L+  G+  S ++++LR QPR+F  +  + +  V    ++GF    
Sbjct: 173 LSRDPNLFLLPNISYLETCGIVGSQLASLLRRQPRIFNLSEEKLRGYVSRALDLGFTLNS 232

Query: 223 LKFVLAVFALRTMSKSTWDKKVEVYRKWGWSEEEIRLAFGRHPWCMMASEDKINGVMDYF 282
              V AV +L ++S+ T+D+KV+++   G+SE+EI     R P  +  SEDK+    +++
Sbjct: 233 RMLVHAVISLSSLSEKTFDRKVKLFMANGFSEDEITDIIRRSPGLIRCSEDKLTLGFEFY 292

Query: 283 VNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKGLI----KKHGNLPLLFDSTE 342
           + ++G E   +A+RP ++S++L+KR++PR  V Q+L  KGL+    KK  N+  + + TE
Sbjct: 293 LKRMGLEREALAKRPCVLSYNLEKRVIPRLKVLQILREKGLLLKEEKKKKNMVGIVEMTE 352

Query: 343 NRFLDKFINPYKERI 350
             FL+K++  + + I
Sbjct: 353 EAFLEKYVVRFGDEI 367

[Close]
BLAST of Lsi05G015230 vs. TAIR 10
Match: AT1G61970.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.8e-32
Identity = 93/341 (27.27%), Postives = 174/341 (51.03%), Query Frame = 0 

Query: 56  KNNEEPLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKL 115
           KNN + ++    +HGF+ SQIS +++ +PQ+L A+ EK+L PKL F QS+G SS EI ++
Sbjct: 81  KNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEI 140

Query: 116 ICGFPFVLKRSLNRQIIPTFDYIQ-ALLQTEEKTIAVIKRF-VGILTWDLRISAGPNIEI 175
           +   P +L +  ++ I   +D+I+  LL+   K   +      G L   +R     N+ +
Sbjct: 141 VSSVPEILGKKGHKTISVYYDFIKDTLLEKSSKNEKLCHSLPQGNLENKIR-----NVSV 200

Query: 176 LKQIGVPDSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSK 235
           L+++G+P   + ++L    +  +    +F+E +++V EMGF+P   KFV A+  +  M++
Sbjct: 201 LRELGMPHKLLFSLLISDSQP-VCGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNE 260

Query: 236 STWDKKVEVY--------------RKW---------------------GWSEEEIRLAFG 295
            T ++KV +Y              +KW                     G+S +E      
Sbjct: 261 KTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVK 320

Query: 296 RHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKG 355
             P C+  S + +    ++ V K+      +   PA+  +SL+KRI+PRG+V + L+SKG
Sbjct: 321 HFPPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKG 380

Query: 356 LIKKH-GNLPLLFDSTENRFLDKFINPY--KERIPGLLKFY 357
           L++    ++  +   T+  FL++++  +  K+ +  L+  Y
Sbjct: 381 LMRNELPSISCVLMCTKQVFLNRYVANHVDKQLVTELMAIY 415

[Close]
BLAST of Lsi05G015230 vs. TAIR 10
Match: AT1G61970.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.8e-32
Identity = 93/341 (27.27%), Postives = 174/341 (51.03%), Query Frame = 0 

Query: 56  KNNEEPLIVFFENHGFSKSQISELVKKFPQVLSANLEKTLLPKLLFFQSKGLSSPEIAKL 115
           KNN + ++    +HGF+ SQIS +++ +PQ+L A+ EK+L PKL F QS+G SS EI ++
Sbjct: 81  KNNPDSVLNLLTSHGFTGSQISTIIRDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEI 140

Query: 116 ICGFPFVLKRSLNRQIIPTFDYIQ-ALLQTEEKTIAVIKRF-VGILTWDLRISAGPNIEI 175
           +   P +L +  ++ I   +D+I+  LL+   K   +      G L   +R     N+ +
Sbjct: 141 VSSVPEILGKKGHKTISVYYDFIKDTLLEKSSKNEKLCHSLPQGNLENKIR-----NVSV 200

Query: 176 LKQIGVPDSNISTILRYQPRVFLTNSIRFKEIVEEVKEMGFNPQRLKFVLAVFALRTMSK 235
           L+++G+P   + ++L    +  +    +F+E +++V EMGF+P   KFV A+  +  M++
Sbjct: 201 LRELGMPHKLLFSLLISDSQP-VCGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNE 260

Query: 236 STWDKKVEVY--------------RKW---------------------GWSEEEIRLAFG 295
            T ++KV +Y              +KW                     G+S +E      
Sbjct: 261 KTIEEKVHLYKSLGFDVGDVWSSFKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVK 320

Query: 296 RHPWCMMASEDKINGVMDYFVNKIGCESSFIARRPALISHSLKKRILPRGSVYQVLLSKG 355
             P C+  S + +    ++ V K+      +   PA+  +SL+KRI+PRG+V + L+SKG
Sbjct: 321 HFPPCIGLSTETVKKKTEFLVKKMNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKG 380

Query: 356 LIKKH-GNLPLLFDSTENRFLDKFINPY--KERIPGLLKFY 357
           L++    ++  +   T+  FL++++  +  K+ +  L+  Y
Sbjct: 381 LMRNELPSISCVLMCTKQVFLNRYVANHVDKQLVTELMAIY 415

The following BLAST results are available for this feature:
BLAST of Lsi05G015230 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZT961.1e-0919.25Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL61.6e-0826.09Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
B6TGN45.2e-0718.63Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
Q6AUK65.7e-0621.97Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
F4IHL38.3e-0524.60Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
BLAST of Lsi05G015230 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EZH04.5e-16382.04uncharacterized protein LOC111440613 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1F4G84.5e-16382.04uncharacterized protein LOC111440613 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I9L41.0e-16281.10transcription termination factor MTERF5, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A6J1GVP22.5e-16180.94uncharacterized protein LOC111457902 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IXF72.7e-16080.39uncharacterized protein LOC111479414 OS=Cucurbita maxima OX=3661 GN=LOC111479414... [more]
BLAST of Lsi05G015230 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022933339.19.3e-16382.04uncharacterized protein LOC111440613 isoform X2 [Cucurbita moschata][more]
XP_022933338.19.3e-16382.04uncharacterized protein LOC111440613 isoform X1 [Cucurbita moschata][more]
XP_022973791.12.1e-16281.10transcription termination factor MTERF5, chloroplastic-like [Cucurbita maxima][more]
XP_022956116.15.1e-16180.94uncharacterized protein LOC111457902 isoform X2 [Cucurbita moschata][more]
XP_022979833.15.6e-16080.39uncharacterized protein LOC111479414 [Cucurbita maxima][more]
BLAST of Lsi05G015230 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G07900.17.8e-6741.09Mitochondrial transcription termination factor family protein [more]
AT1G21150.11.4e-5535.20Mitochondrial transcription termination factor family protein [more]
AT5G64950.18.7e-4229.84Mitochondrial transcription termination factor family protein [more]
AT1G61970.14.8e-3227.27Mitochondrial transcription termination factor family protein [more]
AT1G61970.24.8e-3227.27Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 113..144
e-value: 67.0
score: 12.4
coord: 149..180
e-value: 1300.0
score: 2.9
coord: 286..320
e-value: 1.4
score: 18.0
coord: 77..108
e-value: 2.5E-6
score: 37.0
coord: 185..215
e-value: 61.0
score: 12.5
coord: 254..285
e-value: 1.3
score: 18.1
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 64..223
e-value: 4.0E-21
score: 75.3
coord: 231..337
e-value: 1.4E-12
score: 47.2
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 30..362
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 144..350
e-value: 2.3E-44
score: 153.5
NoneNo IPR availablePANTHERPTHR13068:SF160TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 30..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G015230.1Lsi05G015230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding