Lsi05G000270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G000270
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCationic amino acid transporter like
Locationchr05: 458085 .. 463433 (+)
RNA-Seq ExpressionLsi05G000270
SyntenyLsi05G000270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACTTTCACCTTCATTTCTCTCCATAATAGAGAAGAAGAAGCTTAAGCCCTTTATTCCCTTCACATTAATTCTTCTTCCTCAAGCTCCCACCGCCATGGCCACCGCGGTGTCCTCCACCTTCTTCCGCCGCTACCTCTACTCGTTGTCTCAAACGCCTCACCGACTAAGAAAGCGAATGCTAGCAACATGGACTCCTGATCAAGAACTTAACCAAGTCAGGCAAAGGTCAGGAGCTGACATGAAGAGAAAACTCAAGTGGTTCGATTTAGTTGCTCTCGGCGTCGGGGGAATGCTCGGGGTTGGCGTCTTCGTCACTACTGGTCCCGTCGCCCTCCACGTCACCGGCCCCGCCGTTTTCTTGTCATATATTATCGCTGGAATATCCGCTCTCCTTTCCTCTCTATGTTACACCGAGTTCTCCGTCCATGTCTCCGCCGCTGGTGGCGCCTTCAGTTACTTAAGACTCACTTTCGGTATGATATGAGCACTAATAATGTCAAGTTCACACCACTACAATTTTGGCCTTTATTGGCTACCATTAAAGATAATTTTGTTGACGCTTATTCCCCGTCAACTAATAAAAACACCAGAAATTCATAACTTTGACAGAAAGTTAGGTACTTTTGTGTCAACCAAAGATGTTTTGGTTCATTAACTTAAATATAAATATTTATTAACGCTTTATTACGGTCAAAAAAAATATATATATATATTAATTTTAAATTAATAATTAATTTTAAGCCGTAAATTAATGTTTTATTGACATTTTTTGTCACGAACATCAATAAAGTCCAAATTTGTAGTAGTGACAATTTCAAATTTCAACTACGTGTTTTTAATTACTCTATTTTTTTTTATTTTAATTTTTGGGTTTAATTATAAATTAATGGAGATTTTTTTTTTTTTTTTTAATTTGGTGGTGGTTGTTTGTGAGGTTAATTATATTTATATATATATATATATGATATTTTTGTAGGAGAGTTTGTGGGATATTTTGCTGGAGCAAACATTATAATGGAATATGTGTTGTCAAATGCTGCAGTGGCAAGAAGTTTTACAGAATATCTTTGTGTTGCATTTGGAGAAAGTGAACCAAATGCATGGAGAGTAGAAGTTCATGGTTTGTTAAAGGGTTATAATATGTTGGATTTCCCTGCTGTGGGTCTTATTCTTCTTCTCACTCTTTGTCTTTGCCACAGGTTTTTTCTCTTTTTCTTTTTCTTCTTTTTCTCTCTCTCCATTCACCGTATAAAACTTAATCACGGTTTTCAAATATAGTAAAATAAGTCAAAATATTTACAAACATAACAAAATGTTACTGATAGACTACTATCATATCTATGATCACTGATCGACGGTGACATTTTGCTATATTTGAAAGTATTTTCAGTAGTTTTGTTGTTTAAAATAATTACCCTAAAATTTAATTATAATTACTTTTCTTTTCTTCCAATTTTGCTAAATATAAACTAGTTTTTCTCTATTTAATTGACCTTATAATTATGTGGGTTTCTGTTATATAATTTCAGCCAAAAACTGATAAGAATGTTGTTGAATTTGGGAGTAATATAAAGTAGTAATTACTACTTTTGTTATATTGTGAAAAGGAATGTTGGTTGGAGAGAGAATATAATATATTATTACTCCCCAATTCAACAACATTGGCAAACTTAATTAGTGGACAAAAGCCCAATTTTCTTTTATTATATTATTACTCCCCAATTCAACAACATTGGCAAACTTAATTAGTGGACAAAAGCCCAATTTTCTTTTCCTAGAAATAAATAAAAATTTTGTTATTATAGTTTATAATTAAAATTTAATTTCTATGGTTTGATAAAAGCTTTCTAAATAGTTCTGACGATAAAGTATATTTATAAAGATTTTATTTATAAAGTTTTATCAAATCATAGATACTAAATTTTAACGTTTTCCAAACTTTAACGATCAAATTTGTAATTTAACCTTTCTTTTCTTAAGAAAAGAAAAAAAAACCATATTTCCCAAATTATTGGGGAGAAGACTAGTTTTACCCTATTTGATATATGGGAGAGTATCTACAAAATTATTTAGAATTTGTATCAAATATTTGATTATCACGAAATACTGGTTTTTTCAAATATGTATCAAATGATTATCCATCAATGTCTTAATGAATAATATTTCTCAATTTATCTGGTTTTTTTAGCATACCTATTTTCAAAAGATAGTTTGATCATGGACATTGAAAATCCCAAAAACTCAACTTTTTTTTTTTTTTTACATAGTAAAATAAAATTTGTTAGATAAGAAATGATTAGGTATACCTAATCTCATGCTCCTTTTAATTTTTTACCCAAATAATAATAATAATAAATCATTTAAAAAGATATATATATCACATGACAATTTTTTATTGGACAAGTATACTTTTGTGCAGCCTAAACAACACCTAAATTTTTTCGGTTATGAAATAATAGTAGTAAGATAGGGTTAATTTGGGGTTATTTAAGAAAATTAAAAAAGGTGGTAATTTGTGTTATTGTTTATGCAGTACAAAGGAGAGTTCAATATTAAACCTAATAATGACAGTATTCCATGTGATATTGTTTGGGTTTATAATTGGTTGTGGGATGTACAAAGGAAGTGCAAAGAATTTAGTAAAGCCTGGTGGATTAGCTCCTTTTGGAGTTAAAGGTGTTATGGATGGTGCAGCTATTGTTTACTTCAGCTATATAGGCTATGATTCAGCTTCAACACTTGCTGAAGAAATCCAAAACCCTACAAAAAGCCTTCCAATTGGCATAGTGGGTTCTGTTATCATTACTTCAGCTCTCTATTGTCTCATGGCTCTCTCTCTCTCCCTTATGCTCCCATACAATCAAGTAAGTTATTAATTTAATACTATTTGATCTATGGTAGCCACCTAATTAAGATTTAATATCTTACGGATCTTTTTGACACCTAAATGTTATAGGGTTAGACGGGTTATCCCATGAGATTAGTCGAGGTGTGTGTAAGTTGGCCCGGACACTCACGAATATAAAAAAAAAATATTACTACACTTGGGGTCTTATTACACATTATGTTATTCAATTACTCTAATTTTTTAAGAATGGATGCGACGGGTTTTGAGAAAAACGTGTTTAGTAATTATTCGATGTGTTATATTATTGGAAGGTTTCTGTGTTTGAAAAGTCGGGTGTGTTTTAAGAATTGTGATTAAAGGACTAGTAAAGGAGTTGTGTATTTAAAGAAAAGAAAGATGGGAAAGGTCATAAATGACTTTTTTTTTCTTAGAATATGCTTAAAAAGAGATGAGAAAAGAAACATATTTCTCATTAAAAAAAAAATTATAACCAATATTATAGAAAAATTGTTTTGAAAACAACTATTTATATCCAAAATCATAAGCTTGTGACTTGTGAATAGTGATTGCAAGAATAGAATAAGCTTTTTTACTTAGAGAAACACTAGATAATATAAAATTGATTAAAAAAATATTTTGAAAAATAATCTCTTAAAACATGTAGTCTAAATTTAGATTTTTTTTTAATCCATTTTAACTCATTTTAGACCTATCCAAAGCCATTAAATTAAATTTATTGATACACTAAATAGGTCAAAAAAGAATATTTCACCAAATATATATATATAATATTTATATATATATAAACAAACTTGAACGTCAGGCTTCATTCATCTTTGTACAGCCAACCATCTAAGTATTGAATTATAAATTGTCTTATGACAATTTTTTATTATTATTATTAAAAGAATACATGAAAATGAGTGCAACTAGTATTTTTTTTTATATATGAGTAGCTACCAATCATTATAAATAAAAAATCCTAATTGCTAATGGATTGTGACTAGGAAATAGTCTCTTTCTCCAATTGGGTGATTTATAATTATTATAAACTATATAATTATAAATTTTATGAACAGATATCAGAGAAAGCAGCATTTTCAATAGCATTTCAAAGGATTGGTTGGAAGTGGGCAAGCAATGTTATAGGTGGTGGAGCAAGCTTAGGAATTGTGGCTTCTCTACTTGTTGCTATGTTGGGACAAGCAAGATACCTTTGTGCAATTGGGAGGGCTAGGTTAGTCCCTTTTTGGTTAGCCAAGGTCCATCCTTCAACAGGCACACCACTAAATGCCACACTCTTTTTGGGTAACCCCTTCTCTAAATCTTCTTCTCCTTTTTTTTTTTAATATCACTTTAGTCTCGAGAGTTTTAATTTTGGTTCGTTTTCATTCTTATGCTTTCAAAATGTTCATTTTCGTCTCTATACTTTCAACTTTAGGTCATGTATACTTTTAAAATGTTCATTTTGGCATGACTTTAAAAAAATGATCATTTTAGTCTGAACAATAATTAGGTGCAACCTAAGTAACTCTTATATTGATCTCATACAACCCTTCTTGTTACATAGTAAAAAAAATTAATTCAATCTTTTTTTTAAAAAAAAAAAAGAATAAATTTTTATTAAGTAACTGGGTGGTCTGTACAAAGATATATAAGTGCAGCTCAACCAACACTTAGATACTTTTCTTTTGATCCCTTCATTTTCACTTTTTGAAAACTCAATGACCAAAATGAATCAAAGTTGTAAGTACAAGGACCAAAATGAATATTTTGAAAGTACGGAACCAAAATGAACCAATTTTTGAATTAGAGACCAAAATGAACATTTTGAACGTACAAGGACCAAAATGAACTAAAGCGAAAAATACAAAGACTAAAATAATATTAAATTTTTTTTATTAGTGCCCACTTAAGAAATTCTTGATTGAACCCAAAAATTTATGCTCATAAAATCCAAGTTAATCTTTTGAATATAATCTAATTACATTTCAATGACTATTATTAAGAATATAAATAAAAGAGTTGTGACATAATAAACAAAGTTGGTAGAAAGACAATAAGTGTGAAAATTAAAATGTTAATTATTTCAATCAAACTTGTAATGCAAATCTCTCTTTTTCCTATTGTATAGGACTTTGTACAGCCTCAATTGCACTCTTTACTGAACTTTACATAGTGATTGAAATGATTTCAATTGGGACACTCATGGTGTTCTACCTAGTAGCAAATGCAACCATCTATCGTCGATATGTCATGGTAAGCAAACATCCTCCATCTCGTATTCTCCTCTTCCTCCTCCTCCTTTCATGTTCGGCCATCGGTTTTTCCCTATCGTGGAAGCTCAACCAAGAATGGTGGCCGGGTCTTTTATTCTTCGGTGTATCGACGATCTCCATCATCGCCTTCTTTCACTACAAATTCCCAACCCACAACTCGACCGAGGTGTGGTCGGTGCCCTACATGCCATGGCCGGCAGCGACATCTATATTCCTTAA

mRNA sequence

CTCACTTTCACCTTCATTTCTCTCCATAATAGAGAAGAAGAAGCTTAAGCCCTTTATTCCCTTCACATTAATTCTTCTTCCTCAAGCTCCCACCGCCATGGCCACCGCGGTGTCCTCCACCTTCTTCCGCCGCTACCTCTACTCGTTGTCTCAAACGCCTCACCGACTAAGAAAGCGAATGCTAGCAACATGGACTCCTGATCAAGAACTTAACCAAGTCAGGCAAAGGTCAGGAGCTGACATGAAGAGAAAACTCAAGTGGTTCGATTTAGTTGCTCTCGGCGTCGGGGGAATGCTCGGGGTTGGCGTCTTCGTCACTACTGGTCCCGTCGCCCTCCACGTCACCGGCCCCGCCGTTTTCTTGTCATATATTATCGCTGGAATATCCGCTCTCCTTTCCTCTCTATGTTACACCGAGTTCTCCGTCCATGTCTCCGCCGCTGGTGGCGCCTTCAGTTACTTAAGACTCACTTTCGGAGAGTTTGTGGGATATTTTGCTGGAGCAAACATTATAATGGAATATGTGTTGTCAAATGCTGCAGTGGCAAGAAGTTTTACAGAATATCTTTGTGTTGCATTTGGAGAAAGTGAACCAAATGCATGGAGAGTAGAAGTTCATGGTTTGTTAAAGGGTTATAATATGTTGGATTTCCCTGCTGTGGGTCTTATTCTTCTTCTCACTCTTTGTCTTTGCCACAGTACAAAGGAGAGTTCAATATTAAACCTAATAATGACAGTATTCCATGTGATATTGTTTGGGTTTATAATTGGTTGTGGGATGTACAAAGGAAGTGCAAAGAATTTAGTAAAGCCTGGTGGATTAGCTCCTTTTGGAGTTAAAGGTGTTATGGATGGTGCAGCTATTGTTTACTTCAGCTATATAGGCTATGATTCAGCTTCAACACTTGCTGAAGAAATCCAAAACCCTACAAAAAGCCTTCCAATTGGCATAGTGGGTTCTGTTATCATTACTTCAGCTCTCTATTGTCTCATGGCTCTCTCTCTCTCCCTTATGCTCCCATACAATCAAATATCAGAGAAAGCAGCATTTTCAATAGCATTTCAAAGGATTGGTTGGAAGTGGGCAAGCAATGTTATAGGTGGTGGAGCAAGCTTAGGAATTGTGGCTTCTCTACTTGTTGCTATGTTGGGACAAGCAAGATACCTTTGTGCAATTGGGAGGGCTAGCAAATGCAACCATCTATCGTCGATATGTCATGGTAAGCAAACATCCTCCATCTCGTATTCTCCTCTTCCTCCTCCTCCTTTCATGTTCGGCCATCGGTTTTTCCCTATCGTGGAAGCTCAACCAAGAATGGTGGCCGGGTCTTTTATTCTTCGGTGTATCGACGATCTCCATCATCGCCTTCTTTCACTACAAATTCCCAACCCACAACTCGACCGAGGTGTGGTCGGTGCCCTACATGCCATGGCCGGCAGCGACATCTATATTCCTTAA

Coding sequence (CDS)

ATGGCCACCGCGGTGTCCTCCACCTTCTTCCGCCGCTACCTCTACTCGTTGTCTCAAACGCCTCACCGACTAAGAAAGCGAATGCTAGCAACATGGACTCCTGATCAAGAACTTAACCAAGTCAGGCAAAGGTCAGGAGCTGACATGAAGAGAAAACTCAAGTGGTTCGATTTAGTTGCTCTCGGCGTCGGGGGAATGCTCGGGGTTGGCGTCTTCGTCACTACTGGTCCCGTCGCCCTCCACGTCACCGGCCCCGCCGTTTTCTTGTCATATATTATCGCTGGAATATCCGCTCTCCTTTCCTCTCTATGTTACACCGAGTTCTCCGTCCATGTCTCCGCCGCTGGTGGCGCCTTCAGTTACTTAAGACTCACTTTCGGAGAGTTTGTGGGATATTTTGCTGGAGCAAACATTATAATGGAATATGTGTTGTCAAATGCTGCAGTGGCAAGAAGTTTTACAGAATATCTTTGTGTTGCATTTGGAGAAAGTGAACCAAATGCATGGAGAGTAGAAGTTCATGGTTTGTTAAAGGGTTATAATATGTTGGATTTCCCTGCTGTGGGTCTTATTCTTCTTCTCACTCTTTGTCTTTGCCACAGTACAAAGGAGAGTTCAATATTAAACCTAATAATGACAGTATTCCATGTGATATTGTTTGGGTTTATAATTGGTTGTGGGATGTACAAAGGAAGTGCAAAGAATTTAGTAAAGCCTGGTGGATTAGCTCCTTTTGGAGTTAAAGGTGTTATGGATGGTGCAGCTATTGTTTACTTCAGCTATATAGGCTATGATTCAGCTTCAACACTTGCTGAAGAAATCCAAAACCCTACAAAAAGCCTTCCAATTGGCATAGTGGGTTCTGTTATCATTACTTCAGCTCTCTATTGTCTCATGGCTCTCTCTCTCTCCCTTATGCTCCCATACAATCAAATATCAGAGAAAGCAGCATTTTCAATAGCATTTCAAAGGATTGGTTGGAAGTGGGCAAGCAATGTTATAGGTGGTGGAGCAAGCTTAGGAATTGTGGCTTCTCTACTTGTTGCTATGTTGGGACAAGCAAGATACCTTTGTGCAATTGGGAGGGCTAGCAAATGCAACCATCTATCGTCGATATGTCATGGTAAGCAAACATCCTCCATCTCGTATTCTCCTCTTCCTCCTCCTCCTTTCATGTTCGGCCATCGGTTTTTCCCTATCGTGGAAGCTCAACCAAGAATGGTGGCCGGGTCTTTTATTCTTCGGTGTATCGACGATCTCCATCATCGCCTTCTTTCACTACAAATTCCCAACCCACAACTCGACCGAGGTGTGGTCGGTGCCCTACATGCCATGGCCGGCAGCGACATCTATATTCCTTAA

Protein sequence

MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRASKCNHLSSICHGKQTSSISYSPLPPPPFMFGHRFFPIVEAQPRMVAGSFILRCIDDLHHRLLSLQIPNPQLDRGVVGALHAMAGSDIYIP
Homology
BLAST of Lsi05G000270 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.7e-105
Identity = 201/386 (52.07%), Postives = 273/386 (70.73%), Query Frame = 0

Query: 6   SSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGG 65
           S +  R YL SLS TP RL +R ++  T   E+++VR  SG  M+R L+W+DL+ LG+GG
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 66  MLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLT 125
           M+G GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+T
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 126 FGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDF 185
           FGEF  +F GAN++M+YV+SNAAV+RSFT YL  AFG S  + WR  V GL KG+N +D 
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGIS-TSKWRFVVSGLPKGFNEIDP 193

Query: 186 PAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVK------P 245
            AV ++L++T+ +C ST+ESS +N+IMT FH+    F+I  G  KG +KNL        P
Sbjct: 194 VAVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHP 253

Query: 246 GGLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLM 305
            G  PFG  GV +GAA+VY SYIGYD+ ST+AEE++NP K +P+G+ GSV I + LYCLM
Sbjct: 254 SGFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLM 313

Query: 306 ALSLSLMLPYNQISEKAAFSIAFQ-RIGWKWASNVIGGGASLGIVASLLVAMLGQARYLC 365
           A+S+S++LPY+ I  +A FS AF+   GW+W + V+G GAS GI+ SLLVAMLGQARY+C
Sbjct: 314 AVSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMC 373

Query: 366 AIGRASKCNHLSSICHGKQTSSISYS 385
            IGR+       +  H K ++ ++ S
Sbjct: 374 VIGRSRVVPFWFAKIHPKTSTPVNAS 398

BLAST of Lsi05G000270 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 7.6e-101
Identity = 195/386 (50.52%), Postives = 267/386 (69.17%), Query Frame = 0

Query: 6   SSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGG 65
           S +  R YL SLS TP R  +R ++  T   E+++VR  SG  M+R L+W+DL+ LG+GG
Sbjct: 13  SFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 72

Query: 66  MLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLT 125
           M+G GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+T
Sbjct: 73  MIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 132

Query: 126 FGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDF 185
           FGEF  +  GAN+IM+YVLSNAAV+R FT YL  AFG S  + WR  V GL  G+N +D 
Sbjct: 133 FGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGIS-TSEWRFIVSGLPNGFNEIDP 192

Query: 186 PAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVK------P 245
            AV ++L +T  +C+ST+ESS +N+++T  H+    F+I  G  KG  KNL +      P
Sbjct: 193 IAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENP 252

Query: 246 GGLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLM 305
            G  PFGV GV +GAA+VY SYIGYD+ ST+AEE+++P K +P+GI GSV I   LYCLM
Sbjct: 253 SGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLM 312

Query: 306 ALSLSLMLPYNQISEKAAFSIAFQRI-GWKWASNVIGGGASLGIVASLLVAMLGQARYLC 365
           A+S+S++LPY+ I  +A +S AF +  GW+W + V+G GAS GI+ SL+VAMLGQARY+C
Sbjct: 313 AISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMC 372

Query: 366 AIGRASKCNHLSSICHGKQTSSISYS 385
            IGR+       +  H K ++ ++ S
Sbjct: 373 VIGRSRVVPIWFAKVHPKTSTPVNAS 397

BLAST of Lsi05G000270 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.7e-80
Identity = 154/351 (43.87%), Postives = 229/351 (65.24%), Query Frame = 0

Query: 13  YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGVGVF 72
           YL +L +TP R   R++       E+N+++ RSG +MK+ L W+DL+  G+G ++G G+F
Sbjct: 34  YLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGAVIGSGIF 93

Query: 73  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEFVGY 132
           V TG  A + +GPAV LSY+++G+SA+LS  CYTEF+V +  AGG+F+YLR+  G+F+ +
Sbjct: 94  VLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVELGDFMAF 153

Query: 133 FAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVGLIL 192
            A  NII+EYV+  AAVARS+T Y        +P  +R+ VH L + Y+ LD  AVG+  
Sbjct: 154 IAAGNIILEYVVGGAAVARSWTSYFATLLNH-KPEDFRIIVHKLGEDYSHLDPIAVGVCA 213

Query: 193 LLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKGVMD 252
           ++ +     TK SS  N I ++ H+++  F+I  G  K   KN        P+GV+GV  
Sbjct: 214 IICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---SDFTPYGVRGVFK 273

Query: 253 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPYNQI 312
            AA+++F+YIG+D+ ST+AEE +NP + +PIG+VGS+++T+  YCLMA++L LM PY QI
Sbjct: 274 SAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAVTLCLMQPYQQI 333

Query: 313 SEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRA 364
              A FS+AF  +GW WA  ++  GA  G+   LLV  +GQARY+  I RA
Sbjct: 334 DPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIARA 380

BLAST of Lsi05G000270 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.2e-71
Identity = 153/381 (40.16%), Postives = 231/381 (60.63%), Query Frame = 0

Query: 10  FRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGV 69
           F  Y  +LSQT  R + R+++    + E  +++++S  +MKR L W+DLV  G G ++G 
Sbjct: 25  FGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGSVIGA 84

Query: 70  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEF 129
           G+FV TG  A    GPA+ LSY+++G+SA+LS  CYTEF+V +  AGG+F+YLR+  G+F
Sbjct: 85  GIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIELGDF 144

Query: 130 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVG 189
             +    NI++E ++  AAVAR++T Y       S PNA R++   L  G+N+LD  AV 
Sbjct: 145 AAFITAGNILLESIVGTAAVARAWTSYFATLLNRS-PNALRIKT-DLSSGFNLLDPIAVV 204

Query: 190 LILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKG 249
           +I         ST+++S+LN I +  + ++  F+I  G       NL       PFG +G
Sbjct: 205 VIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP---FLPFGPEG 264

Query: 250 VMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPY 309
           V   AA+VYF+Y G+DS +T+AEE +NP++ +PIG++GS+ I + +YCLMALSLS+M  Y
Sbjct: 265 VFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMALSLSMMQKY 324

Query: 310 NQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRASKCNHL 369
             I   AA+S+AFQ +G KW   ++  GA  G+   LLV  LGQARY+  I R      +
Sbjct: 325 TDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHIARTHMIPPI 384

Query: 370 SSICHGKQTSSISYSPLPPPP 391
            ++ H K  + I+ + L   P
Sbjct: 385 FALVHPKTGTPINANLLVAIP 400

BLAST of Lsi05G000270 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.9e-69
Identity = 152/372 (40.86%), Postives = 228/372 (61.29%), Query Frame = 0

Query: 10  FRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGV 69
           F  Y  +LS T  RL  R+L+  +   EL+  R+ S   M+R L W+DL+ L  G ++G 
Sbjct: 42  FSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGSVVGS 101

Query: 70  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEF 129
           GVFV TG  A    GPAV LSY I+G+SALLS LCY EF V +  AGG+FSYLR+  G+F
Sbjct: 102 GVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVELGDF 161

Query: 130 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVG 189
           + + A  NI++E ++  A + RS++ YL  +  +++ + +R++V    KG+++LD  AV 
Sbjct: 162 IAFIAAGNILLEAMVGAAGLGRSWSSYL-ASLVKNDSDYFRIKVDSFAKGFDLLDPVAVA 221

Query: 190 LILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKG 249
           ++L+        TK +S LNLI ++  V +  FI+  G       NLV      P+G KG
Sbjct: 222 VLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP---FFPYGAKG 281

Query: 250 VMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPY 309
           V+  AA+VY+SY G+D  + +AEE + P++ +PIG+VGS+ + + +YCLMAL+L++M+ Y
Sbjct: 282 VVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMALALTMMVKY 341

Query: 310 NQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRASKCNHL 369
            +I   AA+S+AF +IG KWA  ++G  A  G+  SLLV  LGQARY   I R+      
Sbjct: 342 TEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIARSHMIPPW 401

Query: 370 SSICHGKQTSSI 382
            ++ H K  + I
Sbjct: 402 FALVHPKTGTPI 409

BLAST of Lsi05G000270 vs. ExPASy TrEMBL
Match: A0A0A0LFB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G912900 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.8e-193
Identity = 354/363 (97.52%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAVSSTF RRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA
Sbjct: 1   MATAVSSTFLRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           G+APFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GVAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQISEKAAFSIAFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. ExPASy TrEMBL
Match: A0A5A7T4W3 (Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00360 PE=4 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 4.4e-192
Identity = 351/363 (96.69%), Postives = 356/363 (98.07%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV STF R YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQISEKAAFS+AFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. ExPASy TrEMBL
Match: A0A5D3E636 (Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003830 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.9e-189
Identity = 348/363 (95.87%), Postives = 353/363 (97.25%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV STF R YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQ   KAAFS+AFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQ---KAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 360

BLAST of Lsi05G000270 vs. ExPASy TrEMBL
Match: A0A6J1K670 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491053 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.9e-188
Identity = 343/363 (94.49%), Postives = 353/363 (97.25%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV+S F R Y++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAV LILL+TLCLCHSTKESS LNLIMTVFHVI FGFIIGCGMYKGSA NLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFG KGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLM+PY+QISEKAAFSIAFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. ExPASy TrEMBL
Match: A0A6J1DYJ1 (cationic amino acid transporter 6, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111024699 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.5e-187
Identity = 343/360 (95.28%), Postives = 352/360 (97.78%), Query Frame = 0

Query: 4   AVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGV 63
           A ++ F RRYL+SL+QTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGV
Sbjct: 2   AAAAAFLRRYLHSLAQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGV 61

Query: 64  GGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLR 123
           GGMLGVGVFVTTGPVALHVTGPAVFLSYIIAG+SALLSSLCYTEFSVHVSAAGGAFSYLR
Sbjct: 62  GGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGVSALLSSLCYTEFSVHVSAAGGAFSYLR 121

Query: 124 LTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNML 183
           LTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNML
Sbjct: 122 LTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNML 181

Query: 184 DFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLA 243
           DFPAV LILLLTL LCHSTKESS+LNLIMTVFHVI FGFIIGCGMYKGSAKNLVKPGGLA
Sbjct: 182 DFPAVALILLLTLFLCHSTKESSMLNLIMTVFHVIFFGFIIGCGMYKGSAKNLVKPGGLA 241

Query: 244 PFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSL 303
           PFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNP  SLPIGI+GSVIITSALYCLMALSL
Sbjct: 242 PFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPKHSLPIGILGSVIITSALYCLMALSL 301

Query: 304 SLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRA 363
           SLMLPYNQISEKA+FSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRA
Sbjct: 302 SLMLPYNQISEKASFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRA 361

BLAST of Lsi05G000270 vs. NCBI nr
Match: XP_038887589.1 (cationic amino acid transporter 6, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 689.9 bits (1779), Expect = 1.5e-194
Identity = 356/363 (98.07%), Postives = 360/363 (99.17%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAVSSTF RRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA
Sbjct: 1   MATAVSSTFLRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVI FGFIIGCGMYKGSAKNLVKPG
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVIFFGFIIGCGMYKGSAKNLVKPG 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLM+PYNQISEKAAFSIAFQR+GWKWA N+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYNQISEKAAFSIAFQRLGWKWAGNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. NCBI nr
Match: XP_011652690.1 (cationic amino acid transporter 6, chloroplastic [Cucumis sativus] >KGN60458.1 hypothetical protein Csa_001505 [Cucumis sativus])

HSP 1 Score: 685.3 bits (1767), Expect = 3.7e-193
Identity = 354/363 (97.52%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAVSSTF RRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA
Sbjct: 1   MATAVSSTFLRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           G+APFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GVAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQISEKAAFSIAFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. NCBI nr
Match: KAA0038512.1 (cationic amino acid transporter 6 [Cucumis melo var. makuwa])

HSP 1 Score: 680.6 bits (1755), Expect = 9.0e-192
Identity = 351/363 (96.69%), Postives = 356/363 (98.07%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV STF R YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQISEKAAFS+AFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. NCBI nr
Match: TYK31106.1 (cationic amino acid transporter 6 [Cucumis melo var. makuwa])

HSP 1 Score: 670.2 bits (1728), Expect = 1.2e-188
Identity = 348/363 (95.87%), Postives = 353/363 (97.25%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV STF R YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAVGLILLLTLCLCHSTKESS LNLIMT+FHVI FGFIIGCGMYKGSAKNLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFGVKGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLMLPYNQ   KAAFS+AFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQ---KAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 360

BLAST of Lsi05G000270 vs. NCBI nr
Match: XP_022995554.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 667.9 bits (1722), Expect = 6.1e-188
Identity = 343/363 (94.49%), Postives = 353/363 (97.25%), Query Frame = 0

Query: 1   MATAVSSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60
           MATAV+S F R Y++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPG 240
           NMLDFPAV LILL+TLCLCHSTKESS LNLIMTVFHVI FGFIIGCGMYKGSA NLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 241 GLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300
           GLAPFG KGV+DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAI 360
           LSLSLM+PY+QISEKAAFSIAFQRIGWKWASN+IGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 361 GRA 364
           GRA
Sbjct: 361 GRA 363

BLAST of Lsi05G000270 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 382.5 bits (981), Expect = 4.7e-106
Identity = 201/386 (52.07%), Postives = 273/386 (70.73%), Query Frame = 0

Query: 6   SSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGG 65
           S +  R YL SLS TP RL +R ++  T   E+++VR  SG  M+R L+W+DL+ LG+GG
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 66  MLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLT 125
           M+G GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+T
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 126 FGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDF 185
           FGEF  +F GAN++M+YV+SNAAV+RSFT YL  AFG S  + WR  V GL KG+N +D 
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGIS-TSKWRFVVSGLPKGFNEIDP 193

Query: 186 PAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVK------P 245
            AV ++L++T+ +C ST+ESS +N+IMT FH+    F+I  G  KG +KNL        P
Sbjct: 194 VAVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHP 253

Query: 246 GGLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLM 305
            G  PFG  GV +GAA+VY SYIGYD+ ST+AEE++NP K +P+G+ GSV I + LYCLM
Sbjct: 254 SGFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLM 313

Query: 306 ALSLSLMLPYNQISEKAAFSIAFQ-RIGWKWASNVIGGGASLGIVASLLVAMLGQARYLC 365
           A+S+S++LPY+ I  +A FS AF+   GW+W + V+G GAS GI+ SLLVAMLGQARY+C
Sbjct: 314 AVSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMC 373

Query: 366 AIGRASKCNHLSSICHGKQTSSISYS 385
            IGR+       +  H K ++ ++ S
Sbjct: 374 VIGRSRVVPFWFAKIHPKTSTPVNAS 398

BLAST of Lsi05G000270 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 369.0 bits (946), Expect = 5.4e-102
Identity = 195/386 (50.52%), Postives = 267/386 (69.17%), Query Frame = 0

Query: 6   SSTFFRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGG 65
           S +  R YL SLS TP R  +R ++  T   E+++VR  SG  M+R L+W+DL+ LG+GG
Sbjct: 13  SFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 72

Query: 66  MLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLT 125
           M+G GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+T
Sbjct: 73  MIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 132

Query: 126 FGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDF 185
           FGEF  +  GAN+IM+YVLSNAAV+R FT YL  AFG S  + WR  V GL  G+N +D 
Sbjct: 133 FGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGIS-TSEWRFIVSGLPNGFNEIDP 192

Query: 186 PAVGLILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVK------P 245
            AV ++L +T  +C+ST+ESS +N+++T  H+    F+I  G  KG  KNL +      P
Sbjct: 193 IAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENP 252

Query: 246 GGLAPFGVKGVMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLM 305
            G  PFGV GV +GAA+VY SYIGYD+ ST+AEE+++P K +P+GI GSV I   LYCLM
Sbjct: 253 SGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLM 312

Query: 306 ALSLSLMLPYNQISEKAAFSIAFQRI-GWKWASNVIGGGASLGIVASLLVAMLGQARYLC 365
           A+S+S++LPY+ I  +A +S AF +  GW+W + V+G GAS GI+ SL+VAMLGQARY+C
Sbjct: 313 AISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMC 372

Query: 366 AIGRASKCNHLSSICHGKQTSSISYS 385
            IGR+       +  H K ++ ++ S
Sbjct: 373 VIGRSRVVPIWFAKVHPKTSTPVNAS 397

BLAST of Lsi05G000270 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 299.7 bits (766), Expect = 4.0e-81
Identity = 154/351 (43.87%), Postives = 229/351 (65.24%), Query Frame = 0

Query: 13  YLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGVGVF 72
           YL +L +TP R   R++       E+N+++ RSG +MK+ L W+DL+  G+G ++G G+F
Sbjct: 34  YLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGAVIGSGIF 93

Query: 73  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEFVGY 132
           V TG  A + +GPAV LSY+++G+SA+LS  CYTEF+V +  AGG+F+YLR+  G+F+ +
Sbjct: 94  VLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVELGDFMAF 153

Query: 133 FAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVGLIL 192
            A  NII+EYV+  AAVARS+T Y        +P  +R+ VH L + Y+ LD  AVG+  
Sbjct: 154 IAAGNIILEYVVGGAAVARSWTSYFATLLNH-KPEDFRIIVHKLGEDYSHLDPIAVGVCA 213

Query: 193 LLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKGVMD 252
           ++ +     TK SS  N I ++ H+++  F+I  G  K   KN        P+GV+GV  
Sbjct: 214 IICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---SDFTPYGVRGVFK 273

Query: 253 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPYNQI 312
            AA+++F+YIG+D+ ST+AEE +NP + +PIG+VGS+++T+  YCLMA++L LM PY QI
Sbjct: 274 SAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAVTLCLMQPYQQI 333

Query: 313 SEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRA 364
              A FS+AF  +GW WA  ++  GA  G+   LLV  +GQARY+  I RA
Sbjct: 334 DPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIARA 380

BLAST of Lsi05G000270 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-72
Identity = 153/381 (40.16%), Postives = 231/381 (60.63%), Query Frame = 0

Query: 10  FRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGV 69
           F  Y  +LSQT  R + R+++    + E  +++++S  +MKR L W+DLV  G G ++G 
Sbjct: 25  FGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGSVIGA 84

Query: 70  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEF 129
           G+FV TG  A    GPA+ LSY+++G+SA+LS  CYTEF+V +  AGG+F+YLR+  G+F
Sbjct: 85  GIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIELGDF 144

Query: 130 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVG 189
             +    NI++E ++  AAVAR++T Y       S PNA R++   L  G+N+LD  AV 
Sbjct: 145 AAFITAGNILLESIVGTAAVARAWTSYFATLLNRS-PNALRIKT-DLSSGFNLLDPIAVV 204

Query: 190 LILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKG 249
           +I         ST+++S+LN I +  + ++  F+I  G       NL       PFG +G
Sbjct: 205 VIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP---FLPFGPEG 264

Query: 250 VMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPY 309
           V   AA+VYF+Y G+DS +T+AEE +NP++ +PIG++GS+ I + +YCLMALSLS+M  Y
Sbjct: 265 VFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMALSLSMMQKY 324

Query: 310 NQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRASKCNHL 369
             I   AA+S+AFQ +G KW   ++  GA  G+   LLV  LGQARY+  I R      +
Sbjct: 325 TDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHIARTHMIPPI 384

Query: 370 SSICHGKQTSSISYSPLPPPP 391
            ++ H K  + I+ + L   P
Sbjct: 385 FALVHPKTGTPINANLLVAIP 400

BLAST of Lsi05G000270 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 263.1 bits (671), Expect = 4.2e-70
Identity = 152/372 (40.86%), Postives = 228/372 (61.29%), Query Frame = 0

Query: 10  FRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVALGVGGMLGV 69
           F  Y  +LS T  RL  R+L+  +   EL+  R+ S   M+R L W+DL+ L  G ++G 
Sbjct: 42  FSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGSVVGS 101

Query: 70  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFSYLRLTFGEF 129
           GVFV TG  A    GPAV LSY I+G+SALLS LCY EF V +  AGG+FSYLR+  G+F
Sbjct: 102 GVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVELGDF 161

Query: 130 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGYNMLDFPAVG 189
           + + A  NI++E ++  A + RS++ YL  +  +++ + +R++V    KG+++LD  AV 
Sbjct: 162 IAFIAAGNILLEAMVGAAGLGRSWSSYL-ASLVKNDSDYFRIKVDSFAKGFDLLDPVAVA 221

Query: 190 LILLLTLCLCHSTKESSILNLIMTVFHVILFGFIIGCGMYKGSAKNLVKPGGLAPFGVKG 249
           ++L+        TK +S LNLI ++  V +  FI+  G       NLV      P+G KG
Sbjct: 222 VLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP---FFPYGAKG 281

Query: 250 VMDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMLPY 309
           V+  AA+VY+SY G+D  + +AEE + P++ +PIG+VGS+ + + +YCLMAL+L++M+ Y
Sbjct: 282 VVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMALALTMMVKY 341

Query: 310 NQISEKAAFSIAFQRIGWKWASNVIGGGASLGIVASLLVAMLGQARYLCAIGRASKCNHL 369
            +I   AA+S+AF +IG KWA  ++G  A  G+  SLLV  LGQARY   I R+      
Sbjct: 342 TEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIARSHMIPPW 401

Query: 370 SSICHGKQTSSI 382
            ++ H K  + I
Sbjct: 402 FALVHPKTGTPI 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZ206.7e-10552.07Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SQZ07.6e-10150.52Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q84MA55.7e-8043.87Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 S... [more]
O647592.2e-7140.16Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 S... [more]
Q9SHH05.9e-6940.86Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A0A0LFB21.8e-19397.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G912900 PE=4 SV=1[more]
A0A5A7T4W34.4e-19296.69Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3E6365.9e-18995.87Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1K6702.9e-18894.49cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A6J1DYJ11.5e-18795.28cationic amino acid transporter 6, chloroplastic-like OS=Momordica charantia OX=... [more]
Match NameE-valueIdentityDescription
XP_038887589.11.5e-19498.07cationic amino acid transporter 6, chloroplastic-like isoform X1 [Benincasa hisp... [more]
XP_011652690.13.7e-19397.52cationic amino acid transporter 6, chloroplastic [Cucumis sativus] >KGN60458.1 h... [more]
KAA0038512.19.0e-19296.69cationic amino acid transporter 6 [Cucumis melo var. makuwa][more]
TYK31106.11.2e-18895.87cationic amino acid transporter 6 [Cucumis melo var. makuwa][more]
XP_022995554.16.1e-18894.49cationic amino acid transporter 6, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G04770.14.7e-10652.07cationic amino acid transporter 6 [more]
AT3G10600.15.4e-10250.52cationic amino acid transporter 7 [more]
AT4G21120.14.0e-8143.87amino acid transporter 1 [more]
AT2G34960.11.5e-7240.16cationic amino acid transporter 5 [more]
AT1G17120.14.2e-7040.86cationic amino acid transporter 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 48..379
e-value: 4.7E-80
score: 271.4
NoneNo IPR availablePANTHERPTHR43243:SF3CATIONIC AMINO ACID TRANSPORTER 7, CHLOROPLASTIC-LIKEcoord: 10..364
NoneNo IPR availablePANTHERPTHR43243INNER MEMBRANE TRANSPORTER YGJI-RELATEDcoord: 10..364
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 53..362
e-value: 2.5E-35
score: 122.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G000270.1Lsi05G000270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity