Lsi03G012870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G012870
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerase subunit
Location	chr03: 23686098 .. 23690244 (+)
RNA-Seq Expression	Lsi03G012870
Synteny	Lsi03G012870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTTGCCCAACTTGTGGGAACATGCTGCAATTTGAGTTGCCCAATATGGGTAAATCTTCCAGATTCTTCTGCCCAACTTGTCCATATGTGTCCTACTTGGAGAACAGGGTATGTGTCGCTGATTTTTTTTAGTAGTAAGGCCTCAAGTTGTTTCGCTTTCCTTTTTCGCGTATTAATTTCTTATGTGGTGGCTTAAGGAGTGCATTCTCGTGGTTCTACTATTCAGGTAAAAATCAAAAGAAAGCAACATTTGGTAAAAAAGGAATTAGAACCCATAATCTCTGATGACGATATGAAAAATGCAGCTCAAACCGAAGGTATACATCTTTCCCTTCTAGGTTTTTTTCTCCTCTGTGCTTAGTCTTTGTTTTTGAATTAAAATTTTACCTGCTGGAGTTGCGCAAAAGATATAATCGTATAGCTACTGCAAGGACAATGAACTGATTCACCTTTAAAATTATTCAAATGCTTATGCATATACACGTATATGATAGCCAAACTAGTGTGGCAGTTATATTTCCTTTAATTAAATGAATTACGAAGGCTCACCAAAAGTCAACAAGTAGCTAGAAAGAAAGAATTCTACTGTCTGAGGTCATAATTGAGCTGGAGTATTTTACAAATTGGCAACAATACCATTAACCCACATAATACTGTTTTTATCCTTAAACTTCCTTGAAGCTTAAAAATGAATAGCTTTAAAGCTATTAGGCACAACAAACTTCAAAACATTTCCATCTACACGACCAACACTCCAAAAAAATATGGAGAGTCAGTTAATCCTTCTAAAAGGAAACACCAATTGAGCTAGAGGAACAGTGAAAGCTTGTCCTTGGGATAACATGGTTAGATTCACTGATGTCGAAAAGAAAGATAGATCACTTGAAAACTTTGCACTTTTTGGGAAGCTTTGACTTCCAATCCTTTTTCACAACTTCAATAGGATAGGTGCCTCGCTACGGACTATAGATTGATTAAGATGGTTGACAGATAAATACCTTGAAGGAGTGAGGCTTCATATTCTAGAAATATTGGAGGGAATCATGGATGATATGCACTGGACTGCCCACTCCTCTATTACTATTACCTGTATAGAGGTCCTATGCTTCAAATTACAGCAAAAGTTATGCTTATGCCGCACGTCTTTAGTTGAAATACACCCGTAGATATATAGCACTTTGTCGAATTATGAGGGACTTCTAGGAGTTTTTATCCATACCATAAGAGCAACCCAAAATGAGTAATCTCGCCCGTTTTTCCTTGTCATAATTTTGTGCCGAAGAAAATTGAGTTTCTATCCCATAACCATTGACAAAAGAGAGCTAAACTTTCCTGTCTAATATTACTAATATTTAGGCCTCCATTTGATGAGTAAAAGAGAGCTAAGCTTTGGCTGAATGATGTTCTAGCAACAACTATAAATACCTTGAATTGAGACTCCTTCGAAGGATGGGAGAGATGACACCAAGAAGAAGCCTTTAGATGAGCCAAATCAAACCAAGCAAGAGAACATTGGGAAGAGTTCTGAAAAATGCGGTAGTTCCTCTCCTGCCAAAGTTCAAAAAGTAATGCCTTCACAGCATTCACCCAGAGAAAAAATGCTTGTGAATTCCATCATCTGCTCCCACCTTGGCCAATCTGACCCTGCCAAAAGAAGTTTCAGATGTAACTCGCAATTTCTGGTACAACTTTACTGGAAAATTGGAACATTGAAAGATAATTCAGGAGGAAATGAGAAAGGGGTGGCTGTGCTAAGTAAGACGCCTCCCTTGACAATGAGGGCCCATTCCACTTCATCGACTTGGGGAAATTAATTATTGCCGCCACTCCCCTATTTGATAGAATTATAGAAGTCCAAAAGCAAATTAATAAAACTTCAGAATATCTAGTCCCTTCGGGGACATCCGATAAAACTTCAGAATATCTAGAAATTCGAGATCTAAAAACTTCTATATATGCAACCTTACTTGAACAGGTTTGTTTTATAGGTTATATAACTGTGTTCTTGACTTCTAAGGAGAAAAAAACTTCTATACGTCGATAATAAAACAATATCATTTGTGTTTGTAAAAGGGTGAAATGAATCTTATTTACGGAACCTTCTACCATTTCTGTCATCACGCCTAGGAGAGAAATCTGATAAAAATTAGCAGCCACCAAGAGTGAGAAAAGGGAATATAAGGTCATATTCATGGGGATTTCCTTGACTTGATTCTCGAGAATATCTTGTCTTCTACTATAAGCATTCTCTAAATCAAGCTTAAAGCTGAGTCCTTCCTGGCCGTCTTTCTTCTACACGGGCATTTTGTTTTCATGTATTGTAACTTGAATAGATGATCTACTTCTTCAATCTTCGTCAGACGCTGAGAAAAATAGCTTTTGTCTTTTGTGAATCCTATTAGTGTTACATGGTGCTTCCAAGGTATCTTACTTGGCGTGAGAGGAAGATGTATGCATTAAGTCTGTAAAATCGGACATATCTATCTGAGTGGTTTAACCGTAGTATGTTATGCTAAATTGCTCATTGTAGACAGGTCTGGGTTGTCTAGCCTTAGGAACTTAAATGATTTTGTACAACCAAGGGAAGAACTAGTAGGGGCCGGAATGGAATATCAGTTATAGTCTTGAATTGGATCTGAGGCTCTTTATTAGGATTGGAACAAGCAAGGAATGAGGGACCACTAGCTTCCCTTAAGCGCTCAACATGCATTTAACTCAAAACATGTTTTATCATGTGGCTGAGCAAACGGTAGTTGCACTTGTCTTTGTGAAGAGTGAAGGTGAAGCAACCTAGCATCAGAGTAGAATGGATGACTTGCAACTGAAAGTCTGTTCTTTGAATCAATATATCATACCATGTAATGGAAATCAAATGTTGGTCTAAATTTTGCAGTTTGTAGAATTTGGGCCCTAATGGTGTTCAGTTCGTCACATAACCAGGAGCAACCAATTCCCTTGTCTCTCCCTTTCTGGGAAATGGCTGCCTTCACGAGCATGTGATTGGATGCGTGGGGGACTGTGGAAGCATATGTTGGATAAGCCAGGGGTTTCATGGGACAGTCTAAAAGAGGGAGGGTCGTCCGGTCCAGCAAGAAGATAGAACTCTAATAAAAGTTGTCAATTTGGTCACTTTTGCATAAAATGGAAAGTGGACTGGACGATGACACAAAATTTGTGAAGTTTTTACTGGGGGAAGGAAGAAAATTTTCTGGGTTGTGAACCATCTCTACAACAAGGACACGGGCATTGAATAATAATATGATAATTATTATTATTTTTTAATTTTTTTTCCTATATCCATGAGTGTTCAGGTCAGCTTACGTGTATCTTACATAACAACCCGTCTGATCCTATAACCTTTAGATGTCAAGGAAACTTGTAGGATATTAAATCCTAGGCAAGTGACTACCATGAATTGAACTCATGACCTCTTAGTCTTTTAGTAAGGTCATGTCCCCTTTTTACCTCTAGGCAAACCCATGATGGTTAATATGGTAATTATGATAAAAAGGAATAATACGGTGATTCATTTTTGGAAATTGTAGAACATTTGGACAGCATTATTATCCTGGACAACTTATTATTATTGTTATTCTGAATAGATCTAAAACATTGGCATTGTTTTGTTTTCTGAAATGCATAGGTAGATGATATTATATCACAGAGAATGAGAAGTTTTAGAATGTTGTTCCTTTGTTGTTTATTTCTCTGTTCTCTAAACTGTTTTTGTTTATTGTCCATACTGTAGCAACATGTCCAAATTGCGCGTTTGGCAAGGCTGCTTTTATACAAATACAACTGAGATCAGCTGATGAACCTGCTACAACATTTTACAAGTGCATGAACGAAGATTGTAGACAGAATTGGCGCGAGGATTAAGGGCCAAATTTTTCATTACTGATTTTCTATTTTTTTTTAAATTTTTTTATTTTAACTCATTTGGAGGTAATTTCTATGAATAAATTATTTTTTACGTGAACAGCATTATGAATTTTGTTGGCACTTCGATCATTATTGTATCTCGATAGCTATTTTATTTTCATGTTTTTCTTCTCTTAACATCTTCTCTCGTTCTAATTGAGTCTGCTTTTCCTTATTGACAACGACACTTGATTATTATTATTCTTCTTCTTTTGCTTTA

mRNA sequence

ATGGAGTTTTGCCCAACTTGTGGGAACATGCTGCAATTTGAGTTGCCCAATATGGGTAAATCTTCCAGATTCTTCTGCCCAACTTGTCCATATGTGTCCTACTTGGAGAACAGGGTAAAAATCAAAAGAAAGCAACATTTGGTAAAAAAGGAATTAGAACCCATAATCTCTGATGACGATATGAAAAATGCAGCTCAAACCGAAGGAGTGAGGCTTCATATTCTAGAAATATTGGAGGGAATCATGGATGATATGCACTGGACTGCCCACTCCTCTATTACTATTACCTGTATAGAGGTCCTATGCTTCAAATTACAGCAAAAGTTATGCTTATGCCGCACGAGCAACCAATTCCCTTGTCTCTCCCTTTCTGGGAAATGGCTGCCTTCACGAGCATGTGATTGGATGCGTGGGGGACTGTGGAAGCATATGTTGGATAAGCCAGGGGTTTCATGGGACAGTCTAAAAGAGGGAGGGTCGTCCGCAACATGTCCAAATTGCGCGTTTGGCAAGGCTGCTTTTATACAAATACAACTGAGATCAGCTGATGAACCTGCTACAACATTTTACAAGTGCATGAACGAAGATTGTAGACAGAATTGGCGCGAGGATTAAGGGCCAAATTTTTCATTACTGATTTTCTATTTTTTTTTAAATTTTTTTATTTTAACTCATTTGGAGGTAATTTCTATGAATAAATTATTTTTTACGTGAACAGCATTATGAATTTTGTTGGCACTTCGATCATTATTGTATCTCGATAGCTATTTTATTTTCATGTTTTTCTTCTCTTAACATCTTCTCTCGTTCTAATTGAGTCTGCTTTTCCTTATTGACAACGACACTTGATTATTATTATTCTTCTTCTTTTGCTTTA

Coding sequence (CDS)

ATGGAGTTTTGCCCAACTTGTGGGAACATGCTGCAATTTGAGTTGCCCAATATGGGTAAATCTTCCAGATTCTTCTGCCCAACTTGTCCATATGTGTCCTACTTGGAGAACAGGGTAAAAATCAAAAGAAAGCAACATTTGGTAAAAAAGGAATTAGAACCCATAATCTCTGATGACGATATGAAAAATGCAGCTCAAACCGAAGGAGTGAGGCTTCATATTCTAGAAATATTGGAGGGAATCATGGATGATATGCACTGGACTGCCCACTCCTCTATTACTATTACCTGTATAGAGGTCCTATGCTTCAAATTACAGCAAAAGTTATGCTTATGCCGCACGAGCAACCAATTCCCTTGTCTCTCCCTTTCTGGGAAATGGCTGCCTTCACGAGCATGTGATTGGATGCGTGGGGGACTGTGGAAGCATATGTTGGATAAGCCAGGGGTTTCATGGGACAGTCTAAAAGAGGGAGGGTCGTCCGCAACATGTCCAAATTGCGCGTTTGGCAAGGCTGCTTTTATACAAATACAACTGAGATCAGCTGATGAACCTGCTACAACATTTTACAAGTGCATGAACGAAGATTGTAGACAGAATTGGCGCGAGGATTAA

Protein sequence

MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDDMKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPCLSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED

Homology

[Close]

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot
Match: Q9Y2Y1 (DNA-directed RNA polymerase III subunit RPC10 OS=Homo sapiens OX=9606 GN=POLR3K PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 4.5e-08
Identity = 24/53 (45.28%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 151 SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE 204
           +W+++    ++ +CP C   +A F+Q+Q RSADEP TTFYKC N  C   WR+
Sbjct: 58  AWENV--DSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD 108

[Close]

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot
Match: Q9CQZ7 (DNA-directed RNA polymerase III subunit RPC10 OS=Mus musculus OX=10090 GN=Polr3k PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.6e-08
Identity = 24/53 (45.28%), Postives = 34/53 (64.15%), Query Frame = 0


Query: 151 SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE 204
           +W+++    ++  CP C   +A F+Q+Q RSADEP TTFYKC N  C   WR+
Sbjct: 58  AWENV--DSTAEPCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD 108

[Close]

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot
Match: Q04307 (DNA-directed RNA polymerase III subunit RPC10 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC11 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-07
Identity = 29/66 (43.94%), Postives = 39/66 (59.09%), Query Frame = 0


Query: 142 KHMLDKPGVSWDSLKEGGSSATCPN---CAFGKAAFIQIQLRSADEPATTFYKCMNEDCR 201
           K + D  G  WD++ +  +   CPN   C    A F Q+Q+RSADEP TTFYKC+N  C 
Sbjct: 49  KEVDDVLGGGWDNVDQ--TKTQCPNYDTCGGESAYFFQLQIRSADEPMTTFYKCVN--CG 108

Query: 202 QNWRED 205
             W+E+
Sbjct: 109 HRWKEN 110

[Close]

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot
Match: Q2M2S7 (DNA-directed RNA polymerase III subunit RPC10 OS=Bos taurus OX=9913 GN=POLR3K PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.8e-07
Identity = 23/53 (43.40%), Postives = 33/53 (62.26%), Query Frame = 0


Query: 151 SWDSLKEGGSSATCPNCAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRE 204
           +W+++    ++  CP C   +A F+Q+Q R ADEP TTFYKC N  C   WR+
Sbjct: 58  AWENV--DSTAEPCPKCEHPRAYFMQLQTRYADEPMTTFYKCCNAQCGHRWRD 108

[Close]

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot
Match: O13896 (DNA-directed RNA polymerase III subunit RPC10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpc11 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 22/47 (46.81%), Postives = 30/47 (63.83%), Query Frame = 0


Query: 160 SSATCPN--CAFGKAAFIQIQLRSADEPATTFYKCMNEDCRQNWRED 205
           +  TC N  C   +A F Q+Q+RSADEP +TFY+C    C+  WRE+
Sbjct: 65  TEVTCENTKCDNNRAYFFQLQIRSADEPMSTFYRCTK--CKFQWREN 109

[Close]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL
Match: A0A1S3CQ50 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502981 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 7.8e-48
Identity = 110/204 (53.92%), Postives = 111/204 (54.41%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNCAFGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCAFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPATTFYKCMNE+CRQNWRED
Sbjct: 181 SADEPATTFYKCMNENCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL
Match: A0A0A0LCM4 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_3G844880 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 7.8e-48
Identity = 110/204 (53.92%), Postives = 111/204 (54.41%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNCAFGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCAFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPATTFYKCMNE+CRQNWRED
Sbjct: 181 SADEPATTFYKCMNENCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL
Match: A0A6J1IB59 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111470892 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.7e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNC FGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCGFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPA+TFYKCMNEDCRQNWRED
Sbjct: 181 SADEPASTFYKCMNEDCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL
Match: A0A6J1EXG3 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111439113 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.7e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNC FGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCGFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPA+TFYKCMNEDCRQNWRED
Sbjct: 181 SADEPASTFYKCMNEDCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL
Match: A0A6J1DGL3 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111020260 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.2e-45
Identity = 105/204 (51.47%), Postives = 109/204 (53.43%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYV +LENRVKIKRKQHL+KKELEPIISD+D
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVCHLENRVKIKRKQHLIKKELEPIISDED 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNCAFGKAAFIQ+QLR
Sbjct: 121 -----------------------------------------ATCPNCAFGKAAFIQVQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPATTFYKCMNEDCR NWRED
Sbjct: 181 SADEPATTFYKCMNEDCRINWRED 111

[Close]

BLAST of Lsi03G012870 vs. NCBI nr
Match: XP_004141147.1 (DNA-directed RNA polymerase III subunit RPC10 [Cucumis sativus] >XP_008465337.1 PREDICTED: DNA-directed RNA polymerase III subunit RPC10-like [Cucumis melo] >XP_008465338.1 PREDICTED: DNA-directed RNA polymerase III subunit RPC10-like [Cucumis melo] >KGN59770.1 hypothetical protein Csa_002120 [Cucumis sativus])

HSP 1 Score: 200.3 bits (508), Expect = 1.6e-47
Identity = 110/204 (53.92%), Postives = 111/204 (54.41%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNCAFGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCAFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPATTFYKCMNE+CRQNWRED
Sbjct: 181 SADEPATTFYKCMNENCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. NCBI nr
Match: KAG6597716.1 (DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 38  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 97

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 98  MKNAAQTE---------------------------------------------------- 148

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNC FGKAAFIQIQLR
Sbjct: 158 -----------------------------------------ATCPNCGFGKAAFIQIQLR 148

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPA+TFYKCMNEDCRQNWRED
Sbjct: 218 SADEPASTFYKCMNEDCRQNWRED 148

[Close]

BLAST of Lsi03G012870 vs. NCBI nr
Match: KAG7029162.1 (DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 44  MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 103

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 104 MKNAAQTE---------------------------------------------------- 154

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNC FGKAAFIQIQLR
Sbjct: 164 -----------------------------------------ATCPNCGFGKAAFIQIQLR 154

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPA+TFYKCMNEDCRQNWRED
Sbjct: 224 SADEPASTFYKCMNEDCRQNWRED 154

[Close]

BLAST of Lsi03G012870 vs. NCBI nr
Match: XP_022932603.1 (DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita moschata] >XP_022932604.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita moschata] >XP_022972319.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita maxima] >XP_022972320.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita maxima] >XP_023539106.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita pepo subsp. pepo] >XP_023539107.1 DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNC FGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCGFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPA+TFYKCMNEDCRQNWRED
Sbjct: 181 SADEPASTFYKCMNEDCRQNWRED 111

[Close]

BLAST of Lsi03G012870 vs. NCBI nr
Match: XP_038903069.1 (DNA-directed RNA polymerase III subunit RPC10-like [Benincasa hispida])

HSP 1 Score: 198.4 bits (503), Expect = 6.1e-47
Identity = 109/204 (53.43%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD
Sbjct: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           MKNAAQTE                                                    
Sbjct: 61  MKNAAQTE---------------------------------------------------- 111

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    ATCPNCAFGKAAFIQIQLR
Sbjct: 121 -----------------------------------------ATCPNCAFGKAAFIQIQLR 111

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEPATTFYKCMNE+CR NWRED
Sbjct: 181 SADEPATTFYKCMNENCRHNWRED 111

[Close]

BLAST of Lsi03G012870 vs. TAIR 10
Match: AT4G07950.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-21
Identity = 63/204 (30.88%), Postives = 82/204 (40.20%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGN+L++E    G SSRFFC TCPYV+ +E RV+IK+KQ LVKK +EP+++ DD
Sbjct: 1   MEFCPTCGNLLRYE---GGGSSRFFCSTCPYVANIERRVEIKKKQLLVKKSIEPVVTKDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           +  AA+TE                                                    
Sbjct: 61  IPTAAETE---------------------------------------------------- 106

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    A CP C   KA F  +Q+R
Sbjct: 121 -----------------------------------------APCPRCGHDKAYFKSMQIR 106

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEP + FY+C+   C   WRE+
Sbjct: 181 SADEPESRFYRCLK--CEFTWREE 106

[Close]

BLAST of Lsi03G012870 vs. TAIR 10
Match: AT1G01210.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 95.9 bits (237), Expect = 4.0e-20
Identity = 60/204 (29.41%), Postives = 82/204 (40.20%), Query Frame = 0


Query: 1   MEFCPTCGNMLQFELPNMGKSSRFFCPTCPYVSYLENRVKIKRKQHLVKKELEPIISDDD 60
           MEFCPTCGN+L++E    G +SRFFC TCPYV+Y++ +V+IK+KQ LVKK +E +++ DD
Sbjct: 1   MEFCPTCGNLLRYE---GGGNSRFFCSTCPYVAYIQRQVEIKKKQLLVKKSIEAVVTKDD 60

Query: 61  MKNAAQTEGVRLHILEILEGIMDDMHWTAHSSITITCIEVLCFKLQQKLCLCRTSNQFPC 120
           +  AA+TE                                                    
Sbjct: 61  IPTAAETE---------------------------------------------------- 106

Query: 121 LSLSGKWLPSRACDWMRGGLWKHMLDKPGVSWDSLKEGGSSATCPNCAFGKAAFIQIQLR 180
                                                    A CP C   KA F  +Q+R
Sbjct: 121 -----------------------------------------APCPRCGHDKAYFKSMQIR 106

Query: 181 SADEPATTFYKCMNEDCRQNWRED 205
           SADEP + FY+C+   C   WRE+
Sbjct: 181 SADEPESRFYRCLK--CEFTWREE 106

The following BLAST results are available for this feature:

BLAST of Lsi03G012870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y2Y1	4.5e-08	45.28	DNA-directed RNA polymerase III subunit RPC10 OS=Homo sapiens OX=9606 GN=POLR3K ...	[more]
Q9CQZ7	7.6e-08	45.28	DNA-directed RNA polymerase III subunit RPC10 OS=Mus musculus OX=10090 GN=Polr3k...	[more]
Q04307	1.7e-07	43.94	DNA-directed RNA polymerase III subunit RPC10 OS=Saccharomyces cerevisiae (strai...	[more]
Q2M2S7	3.8e-07	43.40	DNA-directed RNA polymerase III subunit RPC10 OS=Bos taurus OX=9913 GN=POLR3K PE...	[more]
O13896	1.8e-04	46.81	DNA-directed RNA polymerase III subunit RPC10 OS=Schizosaccharomyces pombe (stra...	[more]

BLAST of Lsi03G012870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CQ50	7.8e-48	53.92	DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103502981 PE=3...	[more]
A0A0A0LCM4	7.8e-48	53.92	DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_3G844880 P...	[more]
A0A6J1IB59	1.7e-47	53.43	DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111470892 ...	[more]
A0A6J1EXG3	1.7e-47	53.43	DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11143911...	[more]
A0A6J1DGL3	1.2e-45	51.47	DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC1110202...	[more]

BLAST of Lsi03G012870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141147.1	1.6e-47	53.92	DNA-directed RNA polymerase III subunit RPC10 [Cucumis sativus] >XP_008465337.1 ...	[more]
KAG6597716.1	3.6e-47	53.43	DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma s...	[more]
KAG7029162.1	3.6e-47	53.43	DNA-directed RNA polymerase III subunit RPC10, partial [Cucurbita argyrosperma s...	[more]
XP_022932603.1	3.6e-47	53.43	DNA-directed RNA polymerase III subunit RPC10-like [Cucurbita moschata] >XP_0229...	[more]
XP_038903069.1	6.1e-47	53.43	DNA-directed RNA polymerase III subunit RPC10-like [Benincasa hispida]	[more]

BLAST of Lsi03G012870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G07950.1	3.6e-21	30.88	DNA-directed RNA polymerase, subunit M, archaeal	[more]
AT1G01210.1	4.0e-20	29.41	DNA-directed RNA polymerase, subunit M, archaeal	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001529	DNA-directed RNA polymerase, M/15kDa subunit	SMART	SM00661	rpol9cneu	coord: 2..56 e-value: 7.0E-8 score: 42.2
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 162..203 e-value: 9.6E-14 score: 61.7
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 162..202 e-value: 2.1E-15 score: 56.2
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS00466	ZF_TFIIS_1	coord: 164..201
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS51133	ZF_TFIIS_2	coord: 160..202 score: 12.811053
IPR029401	Nudix hydrolase, N-terminal	PFAM	PF14803	Nudix_N_2	coord: 3..34 e-value: 3.7E-7 score: 30.0
None	No IPR available	GENE3D	2.20.25.10		coord: 151..204 e-value: 4.6E-15 score: 56.8
None	No IPR available	GENE3D	2.20.70.10		coord: 1..34 e-value: 1.9E-6 score: 29.4
None	No IPR available	PANTHER	PTHR11239:SF12	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10	coord: 161..203
None	No IPR available	PANTHER	PTHR11239:SF12	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10	coord: 1..68
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 161..204
IPR012164	DNA-directed RNA polymerase subunit/transcription factor S	PANTHER	PTHR11239	DNA-DIRECTED RNA POLYMERASE	coord: 161..203
IPR012164	DNA-directed RNA polymerase subunit/transcription factor S	PANTHER	PTHR11239	DNA-DIRECTED RNA POLYMERASE	coord: 1..68
IPR019761	DNA-directed RNA polymerase M, 15kDa subunit, conserved site	PROSITE	PS01030	RNA_POL_M_15KD	coord: 3..29
IPR034014	Pol III subunit C11, C-terminal zinc ribbon	CDD	cd10509	Zn-ribbon_RPC11	coord: 162..203 e-value: 9.05412E-19 score: 74.159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G012870.1	Lsi03G012870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0042779	tRNA 3'-trailer cleavage
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding