Lsi02G016400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G016400
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Phorbol-ester/DAG-type domain-containing protein
Location	chr02: 22241005 .. 22241733 (+)
RNA-Seq Expression	Lsi02G016400
Synteny	Lsi02G016400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCAGTTGTGTAATCCAAGGCACCTGGAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAACTAGGCTTCGGGCCACGATACCGATGCAAAAAATGCAATTTTGATCTTCACAAAGCCTGTATGTTTTCACATAAGGCACCAAATTCTCATGAATTCTTTCCGGGTTCCACCTTTGAATTCCTTAGAAACCCACCAGAACCCTGCCATGAGGAATGCAGAATAAAGTGTGATGCTTGCACTAGAACCATCAATGGCTTCGTGTTCCACTGTGACAAACCTGACTTAGATCTCCATCCATGCTGCCTAAACCTAAAAAAGAGTTACCAAATCGAAGATGTGGAGTTCAATCTCCACAAGAAGGTGAAAGGGAAGTGCATGTGGTGCAAGAGGAAGAGCCTTAAAGACGGGGGCTGGTCGTACATCTCTGAGTGCGGCGAGTACCATGTCCATGTGGCTTGTGTTACTGAGATGGCTTTAGAAGAATGGTACAAGAACGGAGGAAACATTGCCGAACATGAACAATCTTTGGCTCTGAAGATGAATCTGAACCAAATTCAAGCTCGAGGGCATGGCAATGGAAGAAGAGGGAACAAGTTTTGGAGAATCCTCAAGATCTTCATCCAGACCATTGTCAGTATTGTTCTGGGAGATCCCACCATGATTCTGACCTCCTTCTTTGTCCAGCTGATTGCATGA

mRNA sequence

ATGGCAAAGCAGTTGTGTAATCCAAGGCACCTGGAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAACTAGGCTTCGGGCCACGATACCGATGCAAAAAATGCAATTTTGATCTTCACAAAGCCTGTATGTTTTCACATAAGGCACCAAATTCTCATGAATTCTTTCCGGGTTCCACCTTTGAATTCCTTAGAAACCCACCAGAACCCTGCCATGAGGAATGCAGAATAAAGTGTGATGCTTGCACTAGAACCATCAATGGCTTCGTGTTCCACTGTGACAAACCTGACTTAGATCTCCATCCATGCTGCCTAAACCTAAAAAAGAGTTACCAAATCGAAGATGTGGAGTTCAATCTCCACAAGAAGGTGAAAGGGAAGTGCATGTGGTGCAAGAGGAAGAGCCTTAAAGACGGGGGCTGGTCGTACATCTCTGAGTGCGGCGAGTACCATGTCCATGTGGCTTGTGTTACTGAGATGGCTTTAGAAGAATGGTACAAGAACGGAGGAAACATTGCCGAACATGAACAATCTTTGGCTCTGAAGATGAATCTGAACCAAATTCAAGCTCGAGGGCATGGCAATGGAAGAAGAGGGAACAAGTTTTGGAGAATCCTCAAGATCTTCATCCAGACCATTGTCAGTATTGTTCTGGGAGATCCCACCATGATTCTGACCTCCTTCTTTGTCCAGCTGATTGCATGA

Coding sequence (CDS)

ATGGCAAAGCAGTTGTGTAATCCAAGGCACCTGGAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAACTAGGCTTCGGGCCACGATACCGATGCAAAAAATGCAATTTTGATCTTCACAAAGCCTGTATGTTTTCACATAAGGCACCAAATTCTCATGAATTCTTTCCGGGTTCCACCTTTGAATTCCTTAGAAACCCACCAGAACCCTGCCATGAGGAATGCAGAATAAAGTGTGATGCTTGCACTAGAACCATCAATGGCTTCGTGTTCCACTGTGACAAACCTGACTTAGATCTCCATCCATGCTGCCTAAACCTAAAAAAGAGTTACCAAATCGAAGATGTGGAGTTCAATCTCCACAAGAAGGTGAAAGGGAAGTGCATGTGGTGCAAGAGGAAGAGCCTTAAAGACGGGGGCTGGTCGTACATCTCTGAGTGCGGCGAGTACCATGTCCATGTGGCTTGTGTTACTGAGATGGCTTTAGAAGAATGGTACAAGAACGGAGGAAACATTGCCGAACATGAACAATCTTTGGCTCTGAAGATGAATCTGAACCAAATTCAAGCTCGAGGGCATGGCAATGGAAGAAGAGGGAACAAGTTTTGGAGAATCCTCAAGATCTTCATCCAGACCATTGTCAGTATTGTTCTGGGAGATCCCACCATGATTCTGACCTCCTTCTTTGTCCAGCTGATTGCATGA

Protein sequence

MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNSHEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQIEDVEFNLHKKVKGKCMWCKRKSLKDGGWSYISECGEYHVHVACVTEMALEEWYKNGGNIAEHEQSLALKMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPTMILTSFFVQLIA

Homology

[Close]

BLAST of Lsi02G016400 vs. ExPASy Swiss-Prot
Match: Q7Y0E8 (Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405500 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.5e-05
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 0


Query: 10  HLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKAC 52
           H EHEL L +    Y CDGC E+G    YRC++C+FDLH  C
Sbjct: 499 HDEHELVLTR-CTTYGCDGCDEMGSSWSYRCRECDFDLHPKC 539

[Close]

BLAST of Lsi02G016400 vs. ExPASy Swiss-Prot
Match: Q7Y0F2 (Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405900 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.2e-05
Identity = 22/45 (48.89%), Postives = 27/45 (60.00%), Query Frame = 0


Query: 12  EHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHK 57
           +HEL L +    Y CDGC E+G    YRCK+C+FDLH  C    K
Sbjct: 511 DHELVLTR-CTTYGCDGCDEMGDSWSYRCKECDFDLHPKCALEEK 554

[Close]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL
Match: A0A5A7T4E4 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00540 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.7e-105
Identity = 177/252 (70.24%), Postives = 208/252 (82.54%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MAKQLC P  L+HELELKKYLKPYTCDGCKE+GFGPRYRC+KC+FDLHKACMF   +P  
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQNSPVF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFFP STF+FL+ PP  CH+EC+  CDACT+ INGF++HC+K  LDLHPCC NL + YQ
Sbjct: 61  HEFFPRSTFKFLKTPPRACHDECKRICDACTKPINGFMYHCEKDQLDLHPCCRNLNRKYQ 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDV+FNL+K+V+ KCMWCK+K++K      GWSYISECGEYHVHVACVTEMALEEWYKN
Sbjct: 121 IEDVKFNLYKEVQRKCMWCKKKTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPT 240
           GG     N A  ++ LAL K+NL  I+ARG GNG +GNK+WRILK+FI+TIVSIVLGDPT
Sbjct: 181 GGGWINANNANQDEQLALQKVNLKAIKARGRGNGGKGNKYWRILKVFIETIVSIVLGDPT 240

Query: 241 MILTSFFVQLIA 243
            +L S FV+LIA
Sbjct: 241 TVLASLFVELIA 252

[Close]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL
Match: A0A0A0KS98 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517070 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.3e-104
Identity = 175/252 (69.44%), Postives = 206/252 (81.75%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MAKQLC P  L+HELELKKYLKPYTCDGCKE+GFGPRYRC+KC+FDLHKACMF   +P  
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQDSPVF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFF  S F+F + PP  CH+EC+  CDACT+ INGF++HC+K DLDLHPCC NL ++YQ
Sbjct: 61  HEFFTRSAFKFSKTPPRACHDECKRICDACTKPINGFMYHCEKDDLDLHPCCRNLNRNYQ 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDV FNL+K+V+GKCMWCK++++K      GWSYISECGEYHVHVACVTEMALEEWYKN
Sbjct: 121 IEDVVFNLYKEVQGKCMWCKKRTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPT 240
           GG     N A  +Q L L K+NL  I+ARG GNG +GNK+WRILK+FI+TIVSIVLGDPT
Sbjct: 181 GGDWMNANNANQDQQLTLQKVNLKAIKARGRGNGGKGNKYWRILKVFIKTIVSIVLGDPT 240

Query: 241 MILTSFFVQLIA 243
            +L S FV+LIA
Sbjct: 241 TVLASLFVELIA 252

[Close]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL
Match: A0A6J1BX53 (uncharacterized protein LOC111006136 OS=Momordica charantia OX=3673 GN=LOC111006136 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.0e-99
Identity = 169/248 (68.15%), Postives = 204/248 (82.26%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MA +  NP H  HELE+KKYLKPYTCDGCKE GFGPRYRC+KC+FDLH+ACMF   + +S
Sbjct: 1   MAMEYRNPSHPNHELEMKKYLKPYTCDGCKETGFGPRYRCEKCDFDLHQACMFPQMSSSS 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFF GS F+F R PP  CH++CR  CDAC + I GFV+HC+K DLDLHPCC NL +SY+
Sbjct: 61  HEFFLGSIFKFFRTPPRACHDKCRRMCDACIKPITGFVYHCEKDDLDLHPCCRNLNRSYR 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDVEFNLHKKV+GKC+WC +K++K    D GWSYIS C +YHVHVACVT+MALEEW+K+
Sbjct: 121 IEDVEFNLHKKVRGKCLWCNKKNIKGSSNDNGWSYISTCEKYHVHVACVTQMALEEWHKS 180

Query: 181 GGN-IAEHEQSLALK-MNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPTMILT 240
           GG+ IA+ +Q LAL+ +NLN+I+ARG G+G  GNKFWRI K+FI+TIVSIVLGDPT IL 
Sbjct: 181 GGDCIADQDQPLALENVNLNEIRARGRGDG--GNKFWRIFKVFIKTIVSIVLGDPTTILA 240

Query: 241 SFFVQLIA 243
           S FV+LIA
Sbjct: 241 SLFVELIA 246

[Close]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL
Match: A0A0A0KNT1 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G516820 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 5.2e-99
Identity = 170/253 (67.19%), Postives = 201/253 (79.45%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           M  QLC P  L+HELELK+YLKPYTCDGCKE+GFGPRYRC+KCNFDLHKACMF   +P  
Sbjct: 1   MPNQLCTPNQLKHELELKEYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPQDSPIF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCD--KPDLDLHPCCLNLKKS 120
           HEFFP S FEF + PP  C ++C+  CDACT+ INGF++HC   K +LDLHPCC NL ++
Sbjct: 61  HEFFPQSAFEFFKTPPRACCDDCKKFCDACTKPINGFMYHCKKVKVELDLHPCCRNLNRN 120

Query: 121 YQIEDVEFNLHKKVKGKCMWCKRKSL----KDGGWSYISECGEYHVHVACVTEMALEEWY 180
           Y+IEDVEFNL+KKVKGKCMWCK++++    +  GWSYISECGEYHVHVACV EMALEEW+
Sbjct: 121 YKIEDVEFNLYKKVKGKCMWCKKRTINPHGQTNGWSYISECGEYHVHVACVIEMALEEWH 180

Query: 181 KNGG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGD 240
           KNGG     N A  +Q LAL K+NL  I+ARG G G +GNK+WRILK+FI TIVSIVLGD
Sbjct: 181 KNGGNWINANNANQDQQLALQKLNLKAIKARGRGKGGKGNKYWRILKVFIGTIVSIVLGD 240

Query: 241 PTMILTSFFVQLI 242
           PT +L S FV+LI
Sbjct: 241 PTTVLVSLFVELI 253

[Close]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL
Match: A0A5A7T8P1 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00550 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.2e-95
Identity = 163/254 (64.17%), Postives = 203/254 (79.92%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MA QLC P  L+H+L+LKKYLKPYTCDGCKE+GFGPRYRC++CNFDLHKACMF   +P  
Sbjct: 1   MANQLCTPNKLKHKLQLKKYLKPYTCDGCKEIGFGPRYRCEECNFDLHKACMFPQDSPTF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHC--DKPDLDLHPCCLNLKKS 120
           HEFFP S FEFL+ PP+ CH++C+  CDACT+TINGF++HC  DK +LDLHPCC NL K+
Sbjct: 61  HEFFPQSAFEFLKTPPKACHDQCKKFCDACTKTINGFMYHCKRDKDELDLHPCCRNLDKN 120

Query: 121 YQIEDVEFNLHKKVKGKCMWCKRKSL----KDGGWSYISECGEYHVHVACVTEMALEEWY 180
           Y+IE+VEFNL  KV+G+CMWCK++++    K  GWSYIS+ G+Y VHVACVTEMALEEW+
Sbjct: 121 YKIENVEFNLSNKVEGECMWCKKETINPHVKSNGWSYISKRGKYLVHVACVTEMALEEWH 180

Query: 181 KNG-----GNIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGD 240
           KNG      N A  +  LAL K+ L  I+ARG G G +GNK+W+++K+FI+TIVSIVLGD
Sbjct: 181 KNGRDWINANNANQDIQLALEKVTLKAIKARGRGEGSKGNKYWKVIKVFIKTIVSIVLGD 240

Query: 241 PTMILTSFFVQLIA 243
           PT +L S FV+LIA
Sbjct: 241 PTTVLASLFVELIA 254

[Close]

BLAST of Lsi02G016400 vs. NCBI nr
Match: KAA0037668.1 (uncharacterized protein E6C27_scaffold277G003360 [Cucumis melo var. makuwa] >TYK22811.1 uncharacterized protein E5676_scaffold311G00540 [Cucumis melo var. makuwa])

HSP 1 Score: 392.1 bits (1006), Expect = 3.4e-105
Identity = 177/252 (70.24%), Postives = 208/252 (82.54%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MAKQLC P  L+HELELKKYLKPYTCDGCKE+GFGPRYRC+KC+FDLHKACMF   +P  
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQNSPVF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFFP STF+FL+ PP  CH+EC+  CDACT+ INGF++HC+K  LDLHPCC NL + YQ
Sbjct: 61  HEFFPRSTFKFLKTPPRACHDECKRICDACTKPINGFMYHCEKDQLDLHPCCRNLNRKYQ 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDV+FNL+K+V+ KCMWCK+K++K      GWSYISECGEYHVHVACVTEMALEEWYKN
Sbjct: 121 IEDVKFNLYKEVQRKCMWCKKKTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPT 240
           GG     N A  ++ LAL K+NL  I+ARG GNG +GNK+WRILK+FI+TIVSIVLGDPT
Sbjct: 181 GGGWINANNANQDEQLALQKVNLKAIKARGRGNGGKGNKYWRILKVFIETIVSIVLGDPT 240

Query: 241 MILTSFFVQLIA 243
            +L S FV+LIA
Sbjct: 241 TVLASLFVELIA 252

[Close]

BLAST of Lsi02G016400 vs. NCBI nr
Match: XP_038891268.1 (uncharacterized protein LOC120080612 [Benincasa hispida])

HSP 1 Score: 390.2 bits (1001), Expect = 1.3e-104
Identity = 176/252 (69.84%), Postives = 210/252 (83.33%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MA+QLCNP HL+HELELKKYLKPYTCDGCKE+GFGPRYRC+KC+FDLHKACMF   +P S
Sbjct: 1   MAEQLCNPSHLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPKNSPIS 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFFP STF+F + PP  CH++CR  CDACT+ INGF++HC K DLDLHPCC NLK +YQ
Sbjct: 61  HEFFPRSTFKFFKIPPRACHDKCRRICDACTKPINGFMYHCKKRDLDLHPCCENLKMNYQ 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLKD----GGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDVEFNLHK+V+G+CMWC +K+++      GWSYISEC +YHVHVAC TEMALEEWYKN
Sbjct: 121 IEDVEFNLHKEVQGECMWCNKKTIRSHGQHNGWSYISECRKYHVHVACATEMALEEWYKN 180

Query: 181 GG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPT 240
           GG     + A+ + +LAL K+NL  IQARG G+G +GNKFWRILK+FI+TIVSIVLGDPT
Sbjct: 181 GGDWINVDNADQDPNLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240

Query: 241 MILTSFFVQLIA 243
            +L S F++LIA
Sbjct: 241 TVLASLFIELIA 252

[Close]

BLAST of Lsi02G016400 vs. NCBI nr
Match: XP_011656238.1 (uncharacterized protein LOC105435689 [Cucumis sativus] >KGN51307.1 hypothetical protein Csa_008103 [Cucumis sativus])

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-103
Identity = 175/252 (69.44%), Postives = 206/252 (81.75%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MAKQLC P  L+HELELKKYLKPYTCDGCKE+GFGPRYRC+KC+FDLHKACMF   +P  
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQDSPVF 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFF  S F+F + PP  CH+EC+  CDACT+ INGF++HC+K DLDLHPCC NL ++YQ
Sbjct: 61  HEFFTRSAFKFSKTPPRACHDECKRICDACTKPINGFMYHCEKDDLDLHPCCRNLNRNYQ 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDV FNL+K+V+GKCMWCK++++K      GWSYISECGEYHVHVACVTEMALEEWYKN
Sbjct: 121 IEDVVFNLYKEVQGKCMWCKKRTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GG-----NIAEHEQSLAL-KMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPT 240
           GG     N A  +Q L L K+NL  I+ARG GNG +GNK+WRILK+FI+TIVSIVLGDPT
Sbjct: 181 GGDWMNANNANQDQQLTLQKVNLKAIKARGRGNGGKGNKYWRILKVFIKTIVSIVLGDPT 240

Query: 241 MILTSFFVQLIA 243
            +L S FV+LIA
Sbjct: 241 TVLASLFVELIA 252

[Close]

BLAST of Lsi02G016400 vs. NCBI nr
Match: KAG6578699.1 (hypothetical protein SDJN03_23147, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 376.7 bits (966), Expect = 1.5e-100
Identity = 178/241 (73.86%), Postives = 196/241 (81.33%), Query Frame = 0


Query: 5   LCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNSHEFF 64
           L NP H  HELELK+Y KPY CDGCKE GFGPRYRC KC+FDLHKACMF+H APNSHEFF
Sbjct: 6   LRNPSHQMHELELKEYKKPYHCDGCKETGFGPRYRCDKCDFDLHKACMFAHNAPNSHEFF 65

Query: 65  PGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQIEDV 124
           PGSTFEFLRNPP+ CHE   ++CDAC +TINGFVFHC++ DLDLHPCC NLK  YQIEDV
Sbjct: 66  PGSTFEFLRNPPKCCHEAI-VRCDACRKTINGFVFHCEEDDLDLHPCCRNLKNRYQIEDV 125

Query: 125 EFNLHKKVKGKCMWCKRKSLKDG----GWSYISECGEYHVHVACVTEMALEEWYKNGGNI 184
           EFNLHK+VKGKCMWCK +  K G    GWSYIS+CG YHVHVACVTEMAL EW++ GGN 
Sbjct: 126 EFNLHKEVKGKCMWCKSRRPKHGGRGNGWSYISDCGNYHVHVACVTEMALNEWHRTGGNT 185

Query: 185 AEHEQSLALKMNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPTMILTSFFVQL 242
           A  E+SLAL MNL +I  R  G G  GNK  RIL+IFIQTIVSI+LGDPTMIL S FVQL
Sbjct: 186 A--EKSLALNMNLKEISRRKKGGG--GNKMLRILEIFIQTIVSIILGDPTMILASLFVQL 241

[Close]

BLAST of Lsi02G016400 vs. NCBI nr
Match: XP_022133597.1 (uncharacterized protein LOC111006136 [Momordica charantia])

HSP 1 Score: 371.3 bits (952), Expect = 6.3e-99
Identity = 169/248 (68.15%), Postives = 204/248 (82.26%), Query Frame = 0


Query: 1   MAKQLCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNS 60
           MA +  NP H  HELE+KKYLKPYTCDGCKE GFGPRYRC+KC+FDLH+ACMF   + +S
Sbjct: 1   MAMEYRNPSHPNHELEMKKYLKPYTCDGCKETGFGPRYRCEKCDFDLHQACMFPQMSSSS 60

Query: 61  HEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQ 120
           HEFF GS F+F R PP  CH++CR  CDAC + I GFV+HC+K DLDLHPCC NL +SY+
Sbjct: 61  HEFFLGSIFKFFRTPPRACHDKCRRMCDACIKPITGFVYHCEKDDLDLHPCCRNLNRSYR 120

Query: 121 IEDVEFNLHKKVKGKCMWCKRKSLK----DGGWSYISECGEYHVHVACVTEMALEEWYKN 180
           IEDVEFNLHKKV+GKC+WC +K++K    D GWSYIS C +YHVHVACVT+MALEEW+K+
Sbjct: 121 IEDVEFNLHKKVRGKCLWCNKKNIKGSSNDNGWSYISTCEKYHVHVACVTQMALEEWHKS 180

Query: 181 GGN-IAEHEQSLALK-MNLNQIQARGHGNGRRGNKFWRILKIFIQTIVSIVLGDPTMILT 240
           GG+ IA+ +Q LAL+ +NLN+I+ARG G+G  GNKFWRI K+FI+TIVSIVLGDPT IL 
Sbjct: 181 GGDCIADQDQPLALENVNLNEIRARGRGDG--GNKFWRIFKVFIKTIVSIVLGDPTTILA 240

Query: 241 SFFVQLIA 243
           S FV+LIA
Sbjct: 241 SLFVELIA 246

[Close]

BLAST of Lsi02G016400 vs. TAIR 10
Match: AT2G16050.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 154.8 bits (390), Expect = 8.6e-38
Identity = 90/244 (36.89%), Postives = 123/244 (50.41%), Query Frame = 0


Query: 10  HLEHELELKKYLKPYTCDGCKELGFGPRYRCK-----KCNFDLHKACMFSHKAPNSHEFF 69
           H +H L+     KP+ CDGC E+G G RYRC       C+FDLH  C     A  +H F+
Sbjct: 11  HPQHNLKFDYTEKPFKCDGCNEVGIGSRYRCSGDNHGSCDFDLHAHCALP-SASITHPFY 70

Query: 70  PGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQIEDV 129
              TF+F+  PP       R  C+AC + ++GF++HC     DLHPCC  L       + 
Sbjct: 71  KKCTFQFVARPP----GNERRYCNACEKDVSGFLYHCKACGFDLHPCCAMLPMVLDDGET 130

Query: 130 EFNLHKKVKGKCMWCKRKSLKDGGWSYISECGEYHVHVACVTEMALEEWYK----NGGNI 189
              L++KV   C  C +K      WSY S C +Y++HVACV EM +E W +      G  
Sbjct: 131 NLFLYRKVSSSCHRCGKKG---RSWSYRSSCKKYNLHVACVREMLVENWRELYTGQHGKS 190

Query: 190 AEHEQSLALKMNLNQIQARGHGNGRRG--NKFWRILKIFIQTIVSIVLGDPTMILTSFFV 243
            EH +SL LK  L       H + R+G   K   I  I +Q ++S VLGDPT ++     
Sbjct: 191 IEH-KSLVLKNTLE----HHHRSSRKGKVQKCCEIAGIAVQFVISAVLGDPTTLIAGVVG 241

[Close]

BLAST of Lsi02G016400 vs. TAIR 10
Match: AT2G37800.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.1e-14
Identity = 47/155 (30.32%), Postives = 69/155 (44.52%), Query Frame = 0


Query: 24  YTCDGCKELGFGPRYRCKKCNFDLHKACMFSHKAPNSHEFF--PGSTFEFLRNPPEPCHE 83
           +TCDGCK  GFG  YRC +C+++LH  C      P++   F  P      +   PE  H+
Sbjct: 163 FTCDGCKTYGFGKTYRCTRCDYNLHDHCA---TCPSTLATFMHPQHELRLVFRGPEHTHQ 222

Query: 84  ECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKSYQIEDVEFNLHK------KVKGK 143
             R+ CD C  +  G  + C+    D+HP C  L +   +  V    H            
Sbjct: 223 NKRM-CDICDESAEGLYYQCEPCGFDVHPLCTQLPQ--HVRHVPHPAHLLELSQWGASSI 282

Query: 144 CMWCKRKSLKDGGWSY-ISECGEYHVHVACVTEMA 170
           CM C R +++   W Y    CG   VH+ C++  A
Sbjct: 283 CMVC-RGAIR--SWRYKCGPCG-LDVHMECISSSA 307

[Close]

BLAST of Lsi02G016400 vs. TAIR 10
Match: AT2G37810.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 65.1 bits (157), Expect = 9.0e-11
Identity = 49/177 (27.68%), Postives = 63/177 (35.59%), Query Frame = 0


Query: 9   RHLEHELELKKY--LKPYTCDGCKELGFGPRYRCKKCNFDLHKAC--------MFSHKAP 68
           +H  H   L K      +TC GCK  GFG  YRC  C++ LH  C         F H   
Sbjct: 12  QHFSHTHPLTKVDEYGEFTCGGCKTYGFGKTYRCAPCDYVLHDHCATCPFTLISFMHPQH 71

Query: 69  NSHEFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCCLNLKKS 128
               F  GS                   C+ C R + G  +HC+    D+HP C    + 
Sbjct: 72  ELRLFVNGS----------------ENMCNICHRLVEGVYYHCETCGFDVHPLCTQHHQH 131

Query: 129 YQIEDVEFNLHK----KVKGKCMWCKRKSLKDGGWSYISECGE--YHVHVACVTEMA 170
                   +L +         CM C+   L    W Y  +CG     VHV CV   A
Sbjct: 132 VSYVPHPAHLLELSQCGASNTCMVCRGAIL---SWRY--KCGPCMLDVHVECVNSSA 167

[Close]

BLAST of Lsi02G016400 vs. TAIR 10
Match: AT2G37780.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.7e-07
Identity = 29/97 (29.90%), Postives = 37/97 (38.14%), Query Frame = 0


Query: 24  YTCDGCKELGFGPRYRCKKCNFDLHKACM--------FSHKAPNSHEFFPGSTFEFLRNP 83
           YTCDGCK  G G  YRC  C++DLH+ C           H   +    F G   E     
Sbjct: 25  YTCDGCKLYGEGRTYRCSDCDYDLHEYCATCPSILLNSCHGPDHELSLFNGHMTE----- 84

Query: 84  PEPCHEECRIKCDACTRTINGFVFHCDKPDLDLHPCC 113
                      C  C  +I G  + C +   + HP C
Sbjct: 85  ---------RSCYVCRVSIQGMFYKCRQCSFEAHPLC 107

[Close]

BLAST of Lsi02G016400 vs. TAIR 10
Match: AT4G14980.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-06
Identity = 36/129 (27.91%), Postives = 57/129 (44.19%), Query Frame = 0


Query: 5   LCNPRHLEHELELKKYLKPYTCDGCKELGFGPRYRCKKCNFDLHKACMFSHK---APNSH 64
           +CNP+H +H L L   L  +TC+ C   G    Y C +CN  +HK C+ +       N H
Sbjct: 122 ICNPKHHKHSLTLLVRLVIFTCNACGVEGDRNPYVCLQCNLMVHKDCIENLPRVICINRH 181

Query: 65  EFFPGSTFEFLRNPPEPCHEECRIKCDACTRTINGF--VFHCDK-PDLDLHPCCLNLKKS 124
           +     TF            E   +C  C +T++     F C + P+  +H  C   K+ 
Sbjct: 182 DHRIFHTFHL-------GQREEHWECGVCRKTVDWVYGAFKCSRCPNYAVHSRCATKKEV 241

The following BLAST results are available for this feature:

BLAST of Lsi02G016400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Y0E8	2.5e-05	52.38	Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405...	[more]
Q7Y0F2	4.2e-05	48.89	Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405...	[more]

BLAST of Lsi02G016400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T4E4	1.7e-105	70.24	Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A0A0KS98	5.3e-104	69.44	Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1BX53	3.0e-99	68.15	uncharacterized protein LOC111006136 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A0A0KNT1	5.2e-99	67.19	Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A5A7T8P1	1.2e-95	64.17	Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=...	[more]

BLAST of Lsi02G016400 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037668.1	3.4e-105	70.24	uncharacterized protein E6C27_scaffold277G003360 [Cucumis melo var. makuwa] >TYK...	[more]
XP_038891268.1	1.3e-104	69.84	uncharacterized protein LOC120080612 [Benincasa hispida]	[more]
XP_011656238.1	1.1e-103	69.44	uncharacterized protein LOC105435689 [Cucumis sativus] >KGN51307.1 hypothetical ...	[more]
KAG6578699.1	1.5e-100	73.86	hypothetical protein SDJN03_23147, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022133597.1	6.3e-99	68.15	uncharacterized protein LOC111006136 [Momordica charantia]	[more]

BLAST of Lsi02G016400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16050.1	8.6e-38	36.89	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37800.1	5.1e-14	30.32	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37810.1	9.0e-11	27.68	Cysteine/Histidine-rich C1 domain family protein	[more]
AT2G37780.1	2.7e-07	29.90	Cysteine/Histidine-rich C1 domain family protein	[more]
AT4G14980.1	1.0e-06	27.91	Cysteine/Histidine-rich C1 domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004146	DC1	PFAM	PF03107	C1_2	coord: 8..52 e-value: 8.9E-10 score: 38.7
None	No IPR available	GENE3D	3.30.60.20		coord: 11..60 e-value: 1.7E-8 score: 36.1
None	No IPR available	PANTHER	PTHR46477	CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN	coord: 8..241
None	No IPR available	PANTHER	PTHR46477:SF5	SUBFAMILY NOT NAMED	coord: 8..241
None	No IPR available	SUPERFAMILY	57889	Cysteine-rich domain	coord: 82..120
None	No IPR available	SUPERFAMILY	57889	Cysteine-rich domain	coord: 7..54
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PROSITE	PS50081	ZF_DAG_PE_2	coord: 12..51 score: 8.533701

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G016400.1	Lsi02G016400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035556	intracellular signal transduction
cellular_component	GO:0016021	integral component of membrane