Lsi02G011280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G011280
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	X8 domain-containing protein
Location	chr02: 13187853 .. 13190417 (+)
RNA-Seq Expression	Lsi02G011280
Synteny	Lsi02G011280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTTACAAGAAGATACTGAAAGCCACTGTGTGTTGTGAAGAAACAAATGTCTTCCTCTACTCATCTCACTATTCTCCATCTCTCTTTGCTTCTAGTTTTTATCATAACTCCAACAATATCAGGTAATTAATCATTTGTTTGATGTCATTTTTGTTTTCGATTTCTTGTTTCTTATCATGAAAACAAACCTGATAAATATAGTTTTAGTGTCTATTTAAGTTCTCGTTTGATAACTATATGTTTGATAATTCATGATCTTCTTTTTATCTAACCAATGTGAAATTTTAATCGCTATCTCAACGAAAGAGAATGTAAAAAAATGTATCCAATGCTTATTTTGATCATTGTTCTTTCTTTTTTAATTACTCCAAAAGATGAACATTGGTGTGAATTTAGTGATCATTTCTTATTTCTTGTTTTTAAAGTCTTATTTATTTAGGTTTGATTTGGTAATGATTTTTTTTAGTATTTTGTTTTTTTAAATTTATGCTGGTATGTTTTCTTCTATATTCTATACTCTCAAATTTATTGAATAAATGATCACTAAATCAAGTAAATTATAGATCATTTGCTTGATGACATTTTTGTTTTCTATTTCTTGTTTCTCGTTTGGAAAATAAAACTATGTTTGATAACTATAGTTTTTTAGTGGTTATTTTAGTTCTTGTTTCGGTCTATTTATTTTTGTTTTAATGTGTTAAAGAAAACATACCTCTCTATATCTTTACGATAGTATGATATTGTCCGCTTTGGACGTAAACCTTCATTTGGCTTTCCTTTTTGTTTCACCTAAAAGGCCTCATACCATTATTGGAGAATAGTTTATCCACTTATCTAATGTGATACTTATTTCCAGTCTTTTCTTCTTTAATTATTTCAAAAGATGAATATTTACAGTGGATTTAGTAATCGTTTTTTATTTCTTGCTTTTAAAGTCTTGCTTATTTAGGTCTCGTTTCGTAAGGATTTTGTTTTTAAGTCTTTTGTTGTTTTCTCCTAAATTCCATACTCCCACATTTATTATGGAAACACTTGAATACTTTGTTAAATTCTGAAAACAAAAATTATTCTTTTTAGTTTTCAAAATTTCGCTTAGTTTTTTAAAACATTAGTGGAAAGTTAATAATAAAACAAAGAAATTTACTAACAGAAATAATATTTATAAACTCAATTTTCAAAAATAAAAAAATAAAAACCAAATGATTATTAAACAAGACCTTATTTATTAATTCTTTTACAAAAGAACAATATTCGTTGCATACTTCACGGAGTTTCTATTAGACTTTTTGAGAATGGAGTTTTTGAAGTGTCTAAAAAATATTAGTGGGATCCACTAGAAAATTCTATGAATCAATAAAACAAGAAACAAAAATGCAATCAAATAATGACACCCGTTTGCACATATTTGTAAGTTACGATGATGGTCGATTAGTAAGGAATCGATTAATGATTGGTGATGGGTGATTTGGGTGTCTAGAGGGTCAATTTGATGAGGAGTGGTGCATAGCTGATGAGCAAGTTCCAGATGATGAGTTGCAAAGAGCTCTTGATTGGGCTTGTGGAAAAGGAGGAGCTGATTGTAGGAATATTCAAATGAAGCAGCCTTGTTTTTACCCAAACACTGTTAGAGATCATGCTTCATATGCTTTCAACAGTTACTACCAAAAGTTCAAGCATAAGGGAGCCACTTGCTACTTCAATTCAGCTGCCATGGTCACTGCTCTTGATCCTAGTAAGTAATAACAATTACTTAACCCTAATTCCGATCTCTTCTCTCGATACCTCTCGTTTAATAACCATCTTCGTTTAGCAATATCGTCGTTCAAAGAGATTAATTTTAATTTACACGTTGAAAACTCTCGTTTGATAACCCATCTTTAACGATTAGCGCTATATATAGTCTTTCTTTTGTTAAATCATTCTTCGATCGAATTCTGGTCATTTGTCACACTTTTTTTATTATTAAAGTTGTTTAGGTCCGATTTGATAACCATTATGTTTTTTAAAATTAAACTTCTAAACACTATTTTCACTTATAGATCAGATGTACTCATCAAACTTCATGCCAAGTTTTAAAACTAAAAAAAGTAGTTCTCGTTTTTAGAATTAGGTTAAGAATTCAAATATTGATGAATATATGATGAAGATCGAAGGAGTGAGAAAACAAGTACAATTTTTCAATAAAATAAAATAAAAAATAAAATAGTTATCAAACCGTCCTTAATTATATATTTTATCTAAAATATTTAATAAATCTTCCTTTTCTTGGCAGGTCATGGTTCATGCAAGTTTGAGTATGTTCCTTGAAGAAGAAGAACATATATAATTGATGATCAAAGATGCTTCGTGATTATAATTTAAGCTTCCCAAAGTTTTTCCAATTCCATTATTGATCTTGTGTACTTGGTTGTGTGAAAGCATTTTCCATTTTCAATTTTTAAGGTAGGCTCCAAGAATCTTTTAGAATCTAAGGTTATTAATCAATTAACTATTGAAGTTGGTTCTTTTTCTTTTATGAGAAAATCAATCAAAGTTTAATGATATGATGTATTGATTGAGTTTG

mRNA sequence

AGATTTACAAGAAGATACTGAAAGCCACTGTGTGTTGTGAAGAAACAAATGTCTTCCTCTACTCATCTCACTATTCTCCATCTCTCTTTGCTTCTAGTTTTTATCATAACTCCAACAATATCAGAGGGTCAATTTGATGAGGAGTGGTGCATAGCTGATGAGCAAGTTCCAGATGATGAGTTGCAAAGAGCTCTTGATTGGGCTTGTGGAAAAGGAGGAGCTGATTGTAGGAATATTCAAATGAAGCAGCCTTGTTTTTACCCAAACACTGTTAGAGATCATGCTTCATATGCTTTCAACAGTTACTACCAAAAGTTCAAGCATAAGGGAGCCACTTGCTACTTCAATTCAGCTGCCATGGTCACTGCTCTTGATCCTAGTCATGGTTCATGCAAGTTTGAGTATGTTCCTTGAAGAAGAAGAACATATATAATTGATGATCAAAGATGCTTCGTGATTATAATTTAAGCTTCCCAAAGTTTTTCCAATTCCATTATTGATCTTGTGTACTTGGTTGTGTGAAAGCATTTTCCATTTTCAATTTTTAAGGTAGGCTCCAAGAATCTTTTAGAATCTAAGGTTATTAATCAATTAACTATTGAAGTTGGTTCTTTTTCTTTTATGAGAAAATCAATCAAAGTTTAATGATATGATGTATTGATTGAGTTTG

Coding sequence (CDS)

ATGTCTTCCTCTACTCATCTCACTATTCTCCATCTCTCTTTGCTTCTAGTTTTTATCATAACTCCAACAATATCAGAGGGTCAATTTGATGAGGAGTGGTGCATAGCTGATGAGCAAGTTCCAGATGATGAGTTGCAAAGAGCTCTTGATTGGGCTTGTGGAAAAGGAGGAGCTGATTGTAGGAATATTCAAATGAAGCAGCCTTGTTTTTACCCAAACACTGTTAGAGATCATGCTTCATATGCTTTCAACAGTTACTACCAAAAGTTCAAGCATAAGGGAGCCACTTGCTACTTCAATTCAGCTGCCATGGTCACTGCTCTTGATCCTAGTCATGGTTCATGCAAGTTTGAGTATGTTCCTTGA

Protein sequence

MSSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP

Homology

[Close]

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 7.2e-22
Identity = 46/91 (50.55%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 31  EEWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKF 90
           ++WCIA  Q    ELQ ALDWACG G  DC  +Q  QPCF P+TV  HASYAFN+YYQ+ 
Sbjct: 389 KKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQS 448

Query: 91  KHKGATCYFNSAAMVTALDPSHGSCKFEYVP 122
                 C FN A++    DPS+G+C +   P
Sbjct: 449 GASSIDCSFNGASVEVDKDPSYGNCLYMIAP 479

[Close]

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 101.7 bits (252), Expect = 6.1e-21
Identity = 52/115 (45.22%), Postives = 69/115 (60.00%), Query Frame = 0


Query: 3   SSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCRN 62
           +ST + +LH+S    F+   T      ++ +CIA + V    LQ ALDWACG G ++C  
Sbjct: 356 NSTPVYLLHVSGSGTFLANDTT-----NQTYCIAMDGVDAKTLQAALDWACGPGRSNCSE 415

Query: 63  IQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKF 118
           IQ  + C+ PN V+ HAS+AFNSYYQK      +C F   AM+T  DPSHGSC F
Sbjct: 416 IQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIF 465

[Close]

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.3e-20
Identity = 42/85 (49.41%), Postives = 56/85 (65.88%), Query Frame = 0


Query: 33  WCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKH 92
           +C+A     DD+L   L+WACG+G A+C  IQ  QPC+ PN V+ HAS+AFN YYQK K 
Sbjct: 362 FCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKS 421

Query: 93  KGATCYFNSAAMVTALDPSHGSCKF 118
            G TC F+  A+ T  DPS+ +C +
Sbjct: 422 AGGTCDFDGTAITTTRDPSYRTCAY 446

[Close]

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 2.0e-19
Identity = 49/117 (41.88%), Postives = 68/117 (58.12%), Query Frame = 0


Query: 3   SSTHLTILHLSLLLVFIITPTISEGQF--DEEWCIADEQVPDDELQRALDWACGKGGADC 62
           S   L+IL  +  ++ +IT     GQ    E WC+A     D  LQ A++WACG+GGADC
Sbjct: 9   SHLRLSILAATAAMLMVITTAQIGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADC 68

Query: 63  RNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKF 118
             IQ   PC  P  V+  AS+ FN+YY K   +   C FN+ A +T+L+PS G+CK+
Sbjct: 69  GPIQQGGPCNDPTDVQKMASFVFNNYYLKNGEEDEACNFNNNAALTSLNPSQGTCKY 125

[Close]

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-17
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 33  WCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKH 92
           WCIA  +  + +L+ ALDWACG G  DC  IQ  QPCF P+T+  HAS+ FNSY+Q+ + 
Sbjct: 369 WCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRA 428

Query: 93  KGATCYFNSAAMVTALDPSHGSC 116
               C F  A +    DPS+  C
Sbjct: 429 TDVACSFGGAGVKVNKDPSYDKC 451

[Close]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL
Match: A0A0A0LW57 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G181450 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.6e-59
Identity = 113/122 (92.62%), Postives = 116/122 (95.08%), Query Frame = 0


Query: 1   MSSSTHLTILHLSLLLVFIITPTIS-EGQFDEEWCIADEQVPDDELQRALDWACGKGGAD 60
           MSSSTHL IL LS LL+FIITPTIS +  FDEEWCIADEQVPDDELQRALDWACGKGGAD
Sbjct: 1   MSSSTHLIILSLSFLLLFIITPTISGDVSFDEEWCIADEQVPDDELQRALDWACGKGGAD 60

Query: 61  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEY 120
           CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVT+LDPSHGSCKFEY
Sbjct: 61  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTSLDPSHGSCKFEY 120

Query: 121 VP 122
           VP
Sbjct: 121 VP 122

[Close]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL
Match: A0A1S3CJN7 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC103501716 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-57
Identity = 110/122 (90.16%), Postives = 115/122 (94.26%), Query Frame = 0


Query: 1   MSSSTHL-TILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGAD 60
           MSSSTHL  IL+LS LL+FIITPTIS+   DEEWCIADEQVPDDELQRALDWACGKGGAD
Sbjct: 5   MSSSTHLIIILYLSFLLLFIITPTISDEWVDEEWCIADEQVPDDELQRALDWACGKGGAD 64

Query: 61  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEY 120
           C NIQMKQPCFYPNT+RDHASYAFNSYYQKFKHKGATCYFNSAAMVT+LDPSHGSCKFEY
Sbjct: 65  CTNIQMKQPCFYPNTLRDHASYAFNSYYQKFKHKGATCYFNSAAMVTSLDPSHGSCKFEY 124

Query: 121 VP 122
           VP
Sbjct: 125 VP 126

[Close]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL
Match: A0A6J1EBZ4 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LOC111432636 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.5e-54
Identity = 104/120 (86.67%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 2   SSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCR 61
           S ST LTILHLS+LL FI+ P  SEGQ  EEWCIADEQVPDDELQRALDWACGKGGADC 
Sbjct: 3   SRSTRLTILHLSVLLAFIVAPRRSEGQL-EEWCIADEQVPDDELQRALDWACGKGGADCG 62

Query: 62  NIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP 121
           +IQ+K+PCFYPNTVRDHASYAFNSYYQKFKHKGA+C+FNSAAMVTALDPSHGSCKFEYVP
Sbjct: 63  DIQVKRPCFYPNTVRDHASYAFNSYYQKFKHKGASCFFNSAAMVTALDPSHGSCKFEYVP 121

[Close]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL
Match: A0A6J1CNC5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Momordica charantia OX=3673 GN=LOC111012685 PE=4 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.5e-54
Identity = 106/121 (87.60%), Postives = 109/121 (90.08%), Query Frame = 0


Query: 1   MSSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADC 60
           MSS   L IL LSLLL+FII P   EGQ  EEWCIADEQVPDDELQRALDWACGKGGADC
Sbjct: 1   MSSPPRLIILQLSLLLLFIIAPQRLEGQL-EEWCIADEQVPDDELQRALDWACGKGGADC 60

Query: 61  RNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYV 120
           R IQ+KQPCFYPNTVRDHASYAFN+YYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYV
Sbjct: 61  RKIQVKQPCFYPNTVRDHASYAFNTYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYV 120

Query: 121 P 122
           P
Sbjct: 121 P 120

[Close]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL
Match: A0A6J1HUR1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC111466373 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.4e-54
Identity = 102/120 (85.00%), Postives = 112/120 (93.33%), Query Frame = 0


Query: 2   SSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCR 61
           S ST LTILHLS+LL F++ P  SEGQF EEWCIADEQVPDDELQRALDWACGKGGA+C 
Sbjct: 3   SRSTRLTILHLSVLLAFLVAPRRSEGQF-EEWCIADEQVPDDELQRALDWACGKGGANCG 62

Query: 62  NIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP 121
           +IQ+K+PCFYP+TVRDHASYAFNSYYQKFKHKGA+C+FNSAAMVTALDPSHGSCKFEYVP
Sbjct: 63  DIQVKRPCFYPDTVRDHASYAFNSYYQKFKHKGASCFFNSAAMVTALDPSHGSCKFEYVP 121

[Close]

BLAST of Lsi02G011280 vs. NCBI nr
Match: XP_038895406.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida])

HSP 1 Score: 238.4 bits (607), Expect = 3.2e-59
Identity = 112/121 (92.56%), Postives = 114/121 (94.21%), Query Frame = 0


Query: 1   MSSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADC 60
           MSSST   IL  SLLLVFIITPT SE QFD+EWCIADEQVPDDELQRALDWACGKGGADC
Sbjct: 1   MSSSTWFIILQFSLLLVFIITPTRSEAQFDQEWCIADEQVPDDELQRALDWACGKGGADC 60

Query: 61  RNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYV 120
           RNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFE+V
Sbjct: 61  RNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEFV 120

Query: 121 P 122
           P
Sbjct: 121 P 121

[Close]

BLAST of Lsi02G011280 vs. NCBI nr
Match: XP_011654946.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucumis sativus] >XP_031738418.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis sativus] >KAE8652861.1 hypothetical protein Csa_013005 [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 5.4e-59
Identity = 113/122 (92.62%), Postives = 116/122 (95.08%), Query Frame = 0


Query: 1   MSSSTHLTILHLSLLLVFIITPTIS-EGQFDEEWCIADEQVPDDELQRALDWACGKGGAD 60
           MSSSTHL IL LS LL+FIITPTIS +  FDEEWCIADEQVPDDELQRALDWACGKGGAD
Sbjct: 5   MSSSTHLIILSLSFLLLFIITPTISGDVSFDEEWCIADEQVPDDELQRALDWACGKGGAD 64

Query: 61  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEY 120
           CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVT+LDPSHGSCKFEY
Sbjct: 65  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTSLDPSHGSCKFEY 124

Query: 121 VP 122
           VP
Sbjct: 125 VP 126

[Close]

BLAST of Lsi02G011280 vs. NCBI nr
Match: XP_008463607.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo])

HSP 1 Score: 232.3 bits (591), Expect = 2.3e-57
Identity = 110/122 (90.16%), Postives = 115/122 (94.26%), Query Frame = 0


Query: 1   MSSSTHL-TILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGAD 60
           MSSSTHL  IL+LS LL+FIITPTIS+   DEEWCIADEQVPDDELQRALDWACGKGGAD
Sbjct: 5   MSSSTHLIIILYLSFLLLFIITPTISDEWVDEEWCIADEQVPDDELQRALDWACGKGGAD 64

Query: 61  CRNIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEY 120
           C NIQMKQPCFYPNT+RDHASYAFNSYYQKFKHKGATCYFNSAAMVT+LDPSHGSCKFEY
Sbjct: 65  CTNIQMKQPCFYPNTLRDHASYAFNSYYQKFKHKGATCYFNSAAMVTSLDPSHGSCKFEY 124

Query: 121 VP 122
           VP
Sbjct: 125 VP 126

[Close]

BLAST of Lsi02G011280 vs. NCBI nr
Match: KAG6573531.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 224.2 bits (570), Expect = 6.2e-55
Identity = 105/120 (87.50%), Postives = 112/120 (93.33%), Query Frame = 0


Query: 2   SSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCR 61
           S ST LTILHLS+LL FI+ P  SEGQF EEWCIADEQVPDDELQRALDWACGKGGADC 
Sbjct: 3   SRSTRLTILHLSVLLAFIVAPRRSEGQF-EEWCIADEQVPDDELQRALDWACGKGGADCG 62

Query: 62  NIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP 121
           +IQ+K+PCFYPNTVRDHASYAFNSYYQKFKHKGA+C+FNSAAMVTALDPSHGSCKFEYVP
Sbjct: 63  DIQVKRPCFYPNTVRDHASYAFNSYYQKFKHKGASCFFNSAAMVTALDPSHGSCKFEYVP 121

[Close]

BLAST of Lsi02G011280 vs. NCBI nr
Match: XP_022925351.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata])

HSP 1 Score: 221.9 bits (564), Expect = 3.1e-54
Identity = 104/120 (86.67%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 2   SSSTHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCR 61
           S ST LTILHLS+LL FI+ P  SEGQ  EEWCIADEQVPDDELQRALDWACGKGGADC 
Sbjct: 3   SRSTRLTILHLSVLLAFIVAPRRSEGQL-EEWCIADEQVPDDELQRALDWACGKGGADCG 62

Query: 62  NIQMKQPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP 121
           +IQ+K+PCFYPNTVRDHASYAFNSYYQKFKHKGA+C+FNSAAMVTALDPSHGSCKFEYVP
Sbjct: 63  DIQVKRPCFYPNTVRDHASYAFNSYYQKFKHKGASCFFNSAAMVTALDPSHGSCKFEYVP 121

[Close]

BLAST of Lsi02G011280 vs. TAIR 10
Match: AT5G35740.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-41
Identity = 77/119 (64.71%), Postives = 93/119 (78.15%), Query Frame = 0


Query: 4   STHLTILHLSLLLVFIITPTISEGQFDEEWCIADEQVPDDELQRALDWACGKGGADCRNI 63
           ST L  + L LL++ +  P  SE +  E+WCIADEQ PDDELQ ALDWACGKGGADC  +
Sbjct: 2   STFLIRILLPLLIIAMTLPRRSEAE-SEQWCIADEQTPDDELQAALDWACGKGGADCSKM 61

Query: 64  QMK-QPCFYPNTVRDHASYAFNSYYQKFKHKGATCYFNSAAMVTALDPSHGSCKFEYVP 122
           Q + QPCF PNT+RDHAS+AFNSYYQ +K+KG +CYF  AAM+T LDPSHGSC++EY P
Sbjct: 62  QQENQPCFLPNTIRDHASFAFNSYYQTYKNKGGSCYFKGAAMITELDPSHGSCQYEYNP 119

[Close]

BLAST of Lsi02G011280 vs. TAIR 10
Match: AT2G04910.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 5.4e-33
Identity = 56/90 (62.22%), Postives = 68/90 (75.56%), Query Frame = 0


Query: 32  EWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFK 91
           +WC+AD Q+PD+ +Q A+DWAC  GGADC  IQ  QPCF PNTV+DHAS  FN+YYQ++K
Sbjct: 14  QWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNNYYQRYK 73

Query: 92  HKGATCYFNSAAMVTALDPSHGSCKFEYVP 122
             G +C FNS A +T  DPSH SC FEYVP
Sbjct: 74  RNGGSCNFNSTAFITQTDPSHDSCHFEYVP 103

[Close]

BLAST of Lsi02G011280 vs. TAIR 10
Match: AT2G04910.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.0e-27
Identity = 48/80 (60.00%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 32  EWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFK 91
           +WC+AD Q+PD+ +Q A+DWAC  GGADC  IQ  QPCF PNTV+DHAS  FN+YYQ++K
Sbjct: 14  QWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNNYYQRYK 73

Query: 92  HKGATCYFNSAAMVTALDPS 112
             G +C FNS A +T  DPS
Sbjct: 74  RNGGSCNFNSTAFITQTDPS 93

[Close]

BLAST of Lsi02G011280 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 5.1e-23
Identity = 50/87 (57.47%), Postives = 59/87 (67.82%), Query Frame = 0


Query: 32  EWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKFK 91
           +WCIA        LQ ALD+ACG GGADC  IQ    C+ PNT+RDHAS+AFNSYYQ  K
Sbjct: 147 QWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQ--K 206

Query: 92  HKGA-TCYFNSAAMVTALDPSHGSCKF 118
           H G+ +C F  AA +T+ DPS GSC F
Sbjct: 207 HPGSDSCNFGGAAQLTSTDPSKGSCHF 231

[Close]

BLAST of Lsi02G011280 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 104.8 bits (260), Expect = 5.1e-23
Identity = 46/91 (50.55%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 31  EEWCIADEQVPDDELQRALDWACGKGGADCRNIQMKQPCFYPNTVRDHASYAFNSYYQKF 90
           ++WCIA  Q    ELQ ALDWACG G  DC  +Q  QPCF P+TV  HASYAFN+YYQ+ 
Sbjct: 389 KKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQS 448

Query: 91  KHKGATCYFNSAAMVTALDPSHGSCKFEYVP 122
                 C FN A++    DPS+G+C +   P
Sbjct: 449 GASSIDCSFNGASVEVDKDPSYGNCLYMIAP 479

The following BLAST results are available for this feature:

BLAST of Lsi02G011280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	7.2e-22	50.55	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
O65399	6.1e-21	45.22	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q94CD8	2.3e-20	49.41	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9M2K6	2.0e-19	41.88	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
Q9FJU9	1.4e-17	45.78	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of Lsi02G011280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW57	2.6e-59	92.62	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G181450 PE=4 SV=...	[more]
A0A1S3CJN7	1.1e-57	90.16	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A6J1EBZ4	1.5e-54	86.67	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CNC5	2.5e-54	87.60	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1HUR1	7.4e-54	85.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of Lsi02G011280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895406.1	3.2e-59	92.56	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida]	[more]
XP_011654946.1	5.4e-59	92.62	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucumis sativus] >XP_031738418.1 PLASMO...	[more]
XP_008463607.1	2.3e-57	90.16	PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo]	[more]
KAG6573531.1	6.2e-55	87.50	PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022925351.1	3.1e-54	86.67	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata]	[more]

BLAST of Lsi02G011280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35740.1	1.9e-41	64.71	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.2	5.4e-33	62.22	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.1	2.0e-27	60.00	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	5.1e-23	57.47	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G29360.1	5.1e-23	50.55	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 32..117 e-value: 5.4E-46 score: 168.8
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 33..104 e-value: 3.3E-21 score: 75.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 28..119 e-value: 8.4E-11 score: 44.1
None	No IPR available	PANTHER	PTHR31044:SF55	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 4	coord: 7..121
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 7..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G011280.1	Lsi02G011280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma