Lsi01G014010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G014010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWAT1-related protein
Locationchr01: 12138098 .. 12142665 (-)
RNA-Seq ExpressionLsi01G014010
SyntenyLsi01G014010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCTTCCTCCTTAAACATGTATTTATGAAGTGGGTTTACGATTCTAATCATGTTTTTATACCTAATAGATTCCCATGAGCTTGAAAGTTTTTAGAAAGAAAAGATGTGTCATATGCTTTATGCATTTTACTTCCTATAAAAGCAGAACCAATATGAGCCATTAAAGAAATTGTACCAACACACCTGTTCTCTCCTCAGCTTCAGCTTCAGCTTCAACATCCATTTAGCAGGATGAATCCCCAAGTTAGCCAATGGTGTGTCTCAGCCAAGCCTTTTTTTGCTGTAGTTTTCTTGCAGTTTGGTCTTGCAGGAATGGACATTCTGTCTAAAGCAGCTCTGAACCAAGGAATGAGCAACTATGTTCTTGTTGTTTATCGCCATGCAGTTGCAACAATTGTTATAGCTCCTTTTGCACTGATTTTTGACAAGTATTTCTCTATTTCCCACTCAGATTTCTTCATTGGCATTTTATCAAACAGTTTACATGCATGCATGAAGTGAACAGCAAAAGATTAATTATTTGAGATGTTTTTTTCTCTTTTCCCAGGAAGGTCAGACCAAAGATGACAATTCCAGTCTTTGCCAAGTTAATGGTCCTAAGCTTGCTAGAGTACTGTCCTGCCTCCCAAAAACTTTTGACAAAATGATTATCTCTCCAACAACATAACATTTTATATCTTCCCTTTTTGGCAGGCCTGTGATTGATCAAAACTTATATTTCTTGGGAATGAAGTACACAACAGCAACTTTTGCAGCTGCCATGTGCAATATTCTCCCTGCCATTACCTTTGTAATGGCTTGGATCCTCAGGTAGTCAATAAGAACATCCATTTTTGGTCTCACCAAACCATATAATCCAAACCCAATCTCTCAGTGAAGTTCCTGTAAATTAAGATTAACACATACTTTTTTAGGCTTGAGAAGGTGAGAATCAAAAGCATCAGAAGCCAAGCTAAGTTAGTGGGAACCATAGCAACAGTTGGAGGAGCCATGATAATGACACTAATAAAAGGCCCAATTTTGGAGCTATTTTGGGTTAAAGAAAGAGCAAACAATCAACAACAAAGAGGTGAAATCAGTCTTCAACATACAATCAAAGGTTCAATTATGATCACAATTGGCTGCTTCAGTTGGGCCTGTTTCATGATTCTTCAGGTACCCTTTTCCCTCTCACAAAATTCCCGAGTCCATAATCCCCTTTGACAAATAACAAAACCCCTTTTGTTGATGGATTCCTCCCATCACAGGATTTGATTCCCATCATTCAGTTTAATAACATGTGACAATTGACAAAAAGAACAACAACAACAACAACAACAACAATAATAGAATCCAACTTTTTCCCACCACTTTTGAGAGGAAAAAGGAATTATAACTCTTCAATAATAATAATAATAATAAAAGATTAAAAAAGTTTTGATAAATCATTTGGAATTTACAGGCAATCACTTTGAAAGCTTACCCAGCTGAGCTTTCCCTTACGGCATGGATCTGCCTGTTGGGCACTGCGGAGGGCACTGTTTTGGCTTTGGTGATGGAAAGAGGAAACGCCGCTGTTTGGTCCATTACTTGGGGAACCAAATTGCTCGCCGCTGTCTATAGTGTAAGCATTTCGCTATTTTTTATAAATCTATTTTAATTTCTTCTGAGTTAAAAGATTAAAAAATCATTCTTAAAAGTAGACTAAATCGTTACAAATTATAAAATGTATAAGCTAAAAATACAAGGACTAAATTGTTACCAACTAAATTTAGAGACTAAATTGTTACTTTCATGAAATAAACTATTATCAATTAAAGTTTAGAGTCTAAATTATTAAAAATTCATAACCTTCTTCTTTCATATTTTGTTTTTATTTTATTTATTTTTTTATATTAATTAATTACTTAATTTTAAATTTAATTTATTATATAACATACGTGGCTTTAATAAGGATGACGAATTATTTATTATTTTTTTAACGATAAATTAATTAATGTTTTGGTAAAGAAAAATCACCTTGTTGAATTTTTTTTCTCTTTATATAAGTCTATGAATATTACCTAAAAGAATTTAATTGAAAAGGTAAACAATTTATTTTGGGAGATAATTAATAGGCTCTTTTCAAATATAGAAAAATGAGTCAAACTATTTATAAATATAGAAAATTTTTACAGTCTATCACTAATAGATCGCGATAGAATTCTATCGCTTTCTATCGCTCAGGTGATAGAATTTATTTGCTTGAACGATAGAAAACGATAAAATTCTATCGCAGTCTATCATTGATAGACAGTGAAATTTTTCTATATTTGTAAATAGTTTGATATTTTTTCTGTTTATAGTAATTTCCCATAATTAATCTTTATGATCTTAATTAATATAAGATAGTTCTAAGATAGATGCGTAGTAATAATTTAAACCCTTGGAAAACAATTGATTGTTATTTTTTTAGTAAATAATAATAATGAAAAAATAATCCGAAAGATGCACCAATTCTAGGGATATGGTACTGTTTTTTTCTTTTTCATTTAGAAAAGTCTTATTAAAATTATACCAAAAGCAAAATTAAAGGAAAAGAATCTCCCCAACCAATATTTATTTGCGTAATTTACATTGACAAGATATAAAAATAACTCTATATCATATGGATATTAGAGAATATCAAAATTTTCTTTTTTTTCTTTTTTTTCTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTAATTCAAGAGAATATCATATTGGTGGTGGTGAAAGTATCTTGTTTTTTTTTCCTTTATTGAAAGTAATATTATTATACATACTTTTATAGCATAGTTTAGTGATAATTAACATATACATCATCCTTTGAAATCAAAGTTTAAATTCTTATTATATATATATGTACACTACAATATTTTGAACAAAAATATGAGTAAAAGAAATTTAAAATATAAAACGAACCATTTACGAAGATTAAGATTATCTATCACATAGGACTTGAAAGTAAATAAAAATAATTTTAAGTTAATGGTCACAAAATTTATTTTATTATTATTTTTTTTAAATATGAAAAATAATTTAAGATATGTATGATTAATAAAATTTTGGGTTTATGGCAGGGGATATTTTGTTCAGGACTTGCTTATTACATTCAAGGATTAGTGATGAAAGACAAAGGACCTGTGTTTGTGACTGCCTTTAGCCCATTGAGTATGGTCATTGTGGCCATTATGAGTTCCTTCATTTTAGGCGAACGTCTTTACTTTGGAAGGTAATTTCCAACCTTTAACTTTTCATTTTTACTGTTACATTAAAAATTTTAAGTAATTAGACTAAAGTAGGGAAAATTTTTAAATATAGAAAAATAAGAAAAACTATTTACACAAAATAGCAAAATTTGTAGATAGTTGTGATAGACACGACACTGATAGAAGTCTAACAAGGTCTATCAGTGATAGAAATGATAGAAGTCTATCACTAATAGATGCTGAAAAATTTATCAATGTCTATCAGTATTTTTTTTTTTTTTTTGCTATTTTCTGTAAATGTTTGACATTTTTTTCTATCTGTAAAAATTTTCCTTAAAGTAATCCAATCCTTTATAAATATCGACCCTCATGAATTTAGAGGTTAAAGTTAATATAGAAAGATTCAACATATCAATGGTCGAATATGCAGAGATCAAACTCAAGTTATATGACTAAGTTATCTTGAAAGATTTAAAGTTCATTATCCAAGATATAAAGTTTAACATAAAGTAGTGATTATTTTTAGGGATAATTTCAAATATAGAAAAATGAATCAAATTATTTACAAATATAGAAAAAATTTACTGTCTATCGCGGTCTTTCGCTGATAGATAGATAGTGAATTTTTTTTATATTTATAAATTGTTTGATATTTTTTCTGTTTATAGTAATTTCTCACACCTTTTTTTTTATTTAGTTCGATAAAATATCTTATTTTATAACGCGTTAAATTAATATTAAACTAAAAAGAAAATAAATAATTTTGCAGGGTACTTGGAGCTGCTGTGATAATTGTGGGATTATACCTTGTTGTTTGGGGCAAAAACAAAGATGGGGATTATTCCTCAAGTGAAGACTTGAAATTGCCCACACAACAAACAAAAGAAATAGAAGACAACAAGATGGAAAGTTTAACGATTGAAGCAATTAATTCAATTGAACTAAAGAATAATATTGAGCAAAAATAATTTAATGAGATTGAAAGGCAAAAATTTATAAAAATAATATTTTAGATTTTGCTGAAATTTTCTAAATAACTTTCCAATTTCCCACAATCCTTTCTATGTCCATCATGTACTACACCACCAAGTGTAACAAACACTTTTTGGTTTCAATTTTCCATATATTTTTATCTAATTTATAACATTAGGG

mRNA sequence

ATTCTTCCTCCTTAAACATGTATTTATGAAGTGGGTTTACGATTCTAATCATGTTTTTATACCTAATAGATTCCCATGAGCTTGAAAGTTTTTAGAAAGAAAAGATGTGTCATATGCTTTATGCATTTTACTTCCTATAAAAGCAGAACCAATATGAGCCATTAAAGAAATTGTACCAACACACCTGTTCTCTCCTCAGCTTCAGCTTCAGCTTCAACATCCATTTAGCAGGATGAATCCCCAAGTTAGCCAATGGTGTGTCTCAGCCAAGCCTTTTTTTGCTGTAGTTTTCTTGCAGTTTGGTCTTGCAGGAATGGACATTCTGTCTAAAGCAGCTCTGAACCAAGGAATGAGCAACTATGTTCTTGTTGTTTATCGCCATGCAGTTGCAACAATTGTTATAGCTCCTTTTGCACTGATTTTTGACAAGAAGGTCAGACCAAAGATGACAATTCCAGTCTTTGCCAAGTTAATGGTCCTAAGCTTGCTAGAGCCTGTGATTGATCAAAACTTATATTTCTTGGGAATGAAGTACACAACAGCAACTTTTGCAGCTGCCATGTGCAATATTCTCCCTGCCATTACCTTTGTAATGGCTTGGATCCTCAGGCTTGAGAAGGTGAGAATCAAAAGCATCAGAAGCCAAGCTAAGTTAGTGGGAACCATAGCAACAGTTGGAGGAGCCATGATAATGACACTAATAAAAGGCCCAATTTTGGAGCTATTTTGGGTTAAAGAAAGAGCAAACAATCAACAACAAAGAGGTGAAATCAGTCTTCAACATACAATCAAAGGTTCAATTATGATCACAATTGGCTGCTTCAGTTGGGCCTGTTTCATGATTCTTCAGGCAATCACTTTGAAAGCTTACCCAGCTGAGCTTTCCCTTACGGCATGGATCTGCCTGTTGGGCACTGCGGAGGGCACTGTTTTGGCTTTGGTGATGGAAAGAGGAAACGCCGCTGTTTGGTCCATTACTTGGGGAACCAAATTGCTCGCCGCTGTCTATAGTGGGATATTTTGTTCAGGACTTGCTTATTACATTCAAGGATTAGTGATGAAAGACAAAGGACCTGTGTTTGTGACTGCCTTTAGCCCATTGAGTATGGTCATTGTGGCCATTATGAGTTCCTTCATTTTAGGCGAACGTCTTTACTTTGGAAGGGTACTTGGAGCTGCTGTGATAATTGTGGGATTATACCTTGTTGTTTGGGGCAAAAACAAAGATGGGGATTATTCCTCAAGTGAAGACTTGAAATTGCCCACACAACAAACAAAAGAAATAGAAGACAACAAGATGGAAAGTTTAACGATTGAAGCAATTAATTCAATTGAACTAAAGAATAATATTGAGCAAAAATAATTTAATGAGATTGAAAGGCAAAAATTTATAAAAATAATATTTTAGATTTTGCTGAAATTTTCTAAATAACTTTCCAATTTCCCACAATCCTTTCTATGTCCATCATGTACTACACCACCAAGTGTAACAAACACTTTTTGGTTTCAATTTTCCATATATTTTTATCTAATTTATAACATTAGGG

Coding sequence (CDS)

ATGAATCCCCAAGTTAGCCAATGGTGTGTCTCAGCCAAGCCTTTTTTTGCTGTAGTTTTCTTGCAGTTTGGTCTTGCAGGAATGGACATTCTGTCTAAAGCAGCTCTGAACCAAGGAATGAGCAACTATGTTCTTGTTGTTTATCGCCATGCAGTTGCAACAATTGTTATAGCTCCTTTTGCACTGATTTTTGACAAGAAGGTCAGACCAAAGATGACAATTCCAGTCTTTGCCAAGTTAATGGTCCTAAGCTTGCTAGAGCCTGTGATTGATCAAAACTTATATTTCTTGGGAATGAAGTACACAACAGCAACTTTTGCAGCTGCCATGTGCAATATTCTCCCTGCCATTACCTTTGTAATGGCTTGGATCCTCAGGCTTGAGAAGGTGAGAATCAAAAGCATCAGAAGCCAAGCTAAGTTAGTGGGAACCATAGCAACAGTTGGAGGAGCCATGATAATGACACTAATAAAAGGCCCAATTTTGGAGCTATTTTGGGTTAAAGAAAGAGCAAACAATCAACAACAAAGAGGTGAAATCAGTCTTCAACATACAATCAAAGGTTCAATTATGATCACAATTGGCTGCTTCAGTTGGGCCTGTTTCATGATTCTTCAGGCAATCACTTTGAAAGCTTACCCAGCTGAGCTTTCCCTTACGGCATGGATCTGCCTGTTGGGCACTGCGGAGGGCACTGTTTTGGCTTTGGTGATGGAAAGAGGAAACGCCGCTGTTTGGTCCATTACTTGGGGAACCAAATTGCTCGCCGCTGTCTATAGTGGGATATTTTGTTCAGGACTTGCTTATTACATTCAAGGATTAGTGATGAAAGACAAAGGACCTGTGTTTGTGACTGCCTTTAGCCCATTGAGTATGGTCATTGTGGCCATTATGAGTTCCTTCATTTTAGGCGAACGTCTTTACTTTGGAAGGGTACTTGGAGCTGCTGTGATAATTGTGGGATTATACCTTGTTGTTTGGGGCAAAAACAAAGATGGGGATTATTCCTCAAGTGAAGACTTGAAATTGCCCACACAACAAACAAAAGAAATAGAAGACAACAAGATGGAAAGTTTAACGATTGAAGCAATTAATTCAATTGAACTAAAGAATAATATTGAGCAAAAATAA

Protein sequence

MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLTIEAINSIELKNNIEQK
Homology
BLAST of Lsi01G014010 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 3.7e-125
Identity = 232/354 (65.54%), Postives = 282/354 (79.66%), Query Frame = 0

Query: 12  AKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPK 71
           A+PF ++V LQ GLAGMDILSKA LN+GMSNYVLVVYRHAVATIV+APFA  FDKKVRPK
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 72  MTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVR 131
           MT+ +F K+ +L LLEPVIDQNLY+LGMKYTTATFA AM N+LPAITFV+A+I  LE+V+
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 132 IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIM 191
           ++ IRS  K+VGT+ATVGGAMIMTL+KGP+L+LFW K    +        +   IKG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTK--GVSAHNTAGTDIHSAIKGAVL 192

Query: 192 ITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWG 251
           +TIGCFS+ACFMILQAITL+ YPAELSLTAWICL+GT EGT +ALVME+GN + W+I W 
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 252 TKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGR 311
           TKLL A YSGI CS LAYY+ G+VMK +GPVFVTAFSPL M+IVAIMS+ I  E++Y GR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 312 VLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTK-EIEDNKMESLTIEAI 365
           VLGA VI  GLYLV+WGK KD  Y+S+  L   + Q K E+  N  +++  E I
Sbjct: 313 VLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSGNGKDNVDHEVI 364

BLAST of Lsi01G014010 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 6.3e-109
Identity = 198/329 (60.18%), Postives = 250/329 (75.99%), Query Frame = 0

Query: 13  KPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPKM 72
           KPF  VV LQFG AG+ I++K ALNQGMS +VL  YRH VATI IAPFA   D+K+RPKM
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 73  TIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVRI 132
           T+ +F K+++L LLEP IDQNLY+ GMKYT+ATF AAM N+LPA  F+MAWI RLEKV +
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 133 KSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIMI 192
           K I SQAK++GTI TVGGAM+MT++KGP++ L W      +Q        Q   KG+ +I
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 193 TIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWGT 252
            IGC  WA F+ LQAITLK+YP ELSLTA+IC LG+ E T++AL +ERGN + W+I   +
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 253 KLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGRV 312
           KLLAAVY G+ CSG+ YY+QG++MK +GPVFVTAF+PLSMVIVAI+ S IL E ++ GR+
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 313 LGAAVIIVGLYLVVWGKNKDGDYSSSEDL 342
           LGA VI++GLY V+WGK+KD   SS  D+
Sbjct: 307 LGAIVIVLGLYSVLWGKSKDEPSSSFSDM 335

BLAST of Lsi01G014010 vs. ExPASy Swiss-Prot
Match: F4IQX1 (WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 8.0e-104
Identity = 201/353 (56.94%), Postives = 245/353 (69.41%), Query Frame = 0

Query: 12  AKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPK 71
           A PF  +V LQ G AGMDIL+K  LN+GMS YVL VYRH VAT+V+APFA  FD      
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD------ 66

Query: 72  MTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVR 131
                           PVI QNL+ LGMKYTTATFA A+ N LPA+TF++A I RLE V+
Sbjct: 67  ---------------NPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVK 126

Query: 132 IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIM 191
            +SIRS AK+VGT+ TVGG M+MTL+KGP L+LFW K    + Q      +  +IKG+++
Sbjct: 127 FQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTK--GPSAQNTVGTDIHSSIKGAVL 186

Query: 192 ITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWG 251
           +TIGCFS+ACFMILQAITLK YPAELSL  WICL+GT EG V+ALVME+GN +VW+I W 
Sbjct: 187 VTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWD 246

Query: 252 TKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGR 311
           TKLL   YSGI CS L YYI G+VMK +GPVFVTAF PL M++VAIMSS I  E++Y GR
Sbjct: 247 TKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGR 306

Query: 312 VLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLTIEAI 365
            LGA VI VGLYLV+WGK KD +Y S+  +     Q    +  +     IE++
Sbjct: 307 ALGATVICVGLYLVIWGKAKDYEYPSTPQIDDDLAQATTSKQKEQRRTVIESV 336

BLAST of Lsi01G014010 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.4e-94
Identity = 179/367 (48.77%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 11  SAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRP 70
           S+KP+FA++ LQFG AGM+I++K +LN GMS+YVLVVYRHA+AT VIAPFA  F++K +P
Sbjct: 15  SSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQP 74

Query: 71  KMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKV 130
           K+T  +F +L +L LL PVIDQN Y++G+KYT+ TF+ AM N+LPA+TF++A + R+E +
Sbjct: 75  KITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEML 134

Query: 131 RIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVK-------ERANNQQQRGEISLQ 190
            +K +  QAK+ GT+ TV GAM+MT+ KGPI+ELFW K         AN    +   S +
Sbjct: 135 DLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDK 194

Query: 191 HTIKGSIMITIGCFSWACFMILQAITLKAYPA-ELSLTAWICLLGTAEGTVLALVMERGN 250
             +KGSI++     +WA   +LQA  LK Y   +LSLT  IC +GT +   +  VME  N
Sbjct: 195 EFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEH-N 254

Query: 251 AAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFI 310
            + W I W   LLAA YSGI  S ++YY+QG+VMK +GPVF TAFSPL MVIVA+M SF+
Sbjct: 255 PSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFV 314

Query: 311 LGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQ--TKEIEDNKMESLT 368
           L E+++ G V+GA +I++GLY V+WGK K+   +  E  K+ +    T+++E N  +   
Sbjct: 315 LAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKI 374

BLAST of Lsi01G014010 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.8e-94
Identity = 175/353 (49.58%), Postives = 255/353 (72.24%), Query Frame = 0

Query: 13  KPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPKM 72
           +P+  ++FLQFG AG  I+  A LNQG + YV++VYR+ VA +V+APFALIF++KVRPKM
Sbjct: 12  RPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRPKM 71

Query: 73  TIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVRI 132
           T+ V  K+M L  LEPV+DQ   +LGM  T+AT+ +A+ NILP++TF++AWILR+EKV I
Sbjct: 72  TLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKVNI 131

Query: 133 KSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHT--IKGSI 192
             +RS+AK++GT+  +GGA++MTL KGP++ L W     + Q      S  H   + G++
Sbjct: 132 AEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVGTL 191

Query: 193 MITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITW 252
           +I +GC +W+ F +LQ+IT+K YPA+LSL+A ICL G  +   +ALV+ER + + W++ W
Sbjct: 192 LILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVER-HPSGWAVGW 251

Query: 253 GTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFG 312
             +L A +Y+GI  SG+ YY+QG+VMK +GPVFVTAF+PL M++VA+++SFIL E+++FG
Sbjct: 252 DARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHFG 311

Query: 313 RVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQ-----TKEIEDNKMES 359
            V+G AVI  GLY+VVWGK KD + S  + L+  + Q     TK  +DNK+ S
Sbjct: 312 CVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDDNKLVS 363

BLAST of Lsi01G014010 vs. ExPASy TrEMBL
Match: A0A6J1CRJ2 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111013601 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 357/376 (94.95%), Postives = 367/376 (97.61%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MN Q+SQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNVQISQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIP+FAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPIFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKV+IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERAN+QQQRGEI
Sbjct: 121 MAWILRLEKVKIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANHQQQRGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGR+LGA VIIVGLYLVVWGKNKDG+ SSSE+LKLPT+QT+EIE NKMESLT
Sbjct: 301 FILGERLYFGRILGAGVIIVGLYLVVWGKNKDGN-SSSEELKLPTKQTREIEANKMESLT 360

Query: 361 IEAINSIELKNNIEQK 377
           IEA NS  L+N  EQK
Sbjct: 361 IEASNSSALQN--EQK 373

BLAST of Lsi01G014010 vs. ExPASy TrEMBL
Match: A0A0A0KX58 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_4G293840 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.4e-188
Identity = 352/377 (93.37%), Postives = 364/377 (96.55%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYF+GMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFMGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGT+ATVGGAMIMTL+KGPI+ELFWVKERANN QQRGEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTVATVGGAMIMTLMKGPIVELFWVKERANNPQQRGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GN AVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNPAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNK-MESL 360
           FILGERLYFGRVLGA VIIVGLYLVVWGKNKD + SSSEDLKLPT+Q   +EDNK ME+L
Sbjct: 301 FILGERLYFGRVLGAVVIIVGLYLVVWGKNKDENCSSSEDLKLPTKQI--LEDNKTMETL 360

Query: 361 TIEAINSIELKNNIEQK 377
           TIE  + I+LKNN EQK
Sbjct: 361 TIEPNSCIDLKNNNEQK 375

BLAST of Lsi01G014010 vs. ExPASy TrEMBL
Match: A0A5A7TZF9 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001680 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.0e-186
Identity = 351/376 (93.35%), Postives = 360/376 (95.74%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MN QVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNSQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGT+ATVGGAMIMTL+KGPILELFWVKER  N QQ GEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTVATVGGAMIMTLMKGPILELFWVKERGTNPQQSGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GN AVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNPAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGRVLGA VIIVGLYLVVWGKNKD + SS+EDLKLPT+QT  IEDNKMESLT
Sbjct: 301 FILGERLYFGRVLGAVVIIVGLYLVVWGKNKDENCSSNEDLKLPTKQT--IEDNKMESLT 360

Query: 361 IEAINSIELKNNIEQK 377
           IE    ++LK NIEQK
Sbjct: 361 IEPNICVDLK-NIEQK 373

BLAST of Lsi01G014010 vs. ExPASy TrEMBL
Match: A0A1S3BIL9 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103489968 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.0e-186
Identity = 351/376 (93.35%), Postives = 360/376 (95.74%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MN QVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNSQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGT+ATVGGAMIMTL+KGPILELFWVKER  N QQ GEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTVATVGGAMIMTLMKGPILELFWVKERGTNPQQSGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GN AVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNPAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGRVLGA VIIVGLYLVVWGKNKD + SS+EDLKLPT+QT  IEDNKMESLT
Sbjct: 301 FILGERLYFGRVLGAVVIIVGLYLVVWGKNKDENCSSNEDLKLPTKQT--IEDNKMESLT 360

Query: 361 IEAINSIELKNNIEQK 377
           IE    ++LK NIEQK
Sbjct: 361 IEPNICVDLK-NIEQK 373

BLAST of Lsi01G014010 vs. ExPASy TrEMBL
Match: A0A6J1ISL3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478038 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.2e-181
Identity = 336/367 (91.55%), Postives = 353/367 (96.19%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MNPQV QWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 35  MNPQVGQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 94

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIP+FAKLM+LSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 95  ALIFDKKVRPKMTIPIFAKLMILSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 154

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKV+IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVK+RA++ QQRG+I
Sbjct: 155 MAWILRLEKVKIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKQRADHAQQRGDI 214

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER
Sbjct: 215 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 274

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GNAAVWSI WGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 275 GNAAVWSIGWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 334

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGR+LGA VIIVGLYLVVWGKNKDGD S +EDLKLPT+QT E+  +   S +
Sbjct: 335 FILGERLYFGRILGAGVIIVGLYLVVWGKNKDGD-SLNEDLKLPTKQTTEMHKS---SSS 394

Query: 361 IEAINSI 368
           ++ +NS+
Sbjct: 395 VQELNSV 397

BLAST of Lsi01G014010 vs. NCBI nr
Match: XP_038883137.1 (WAT1-related protein At2g37460 [Benincasa hispida])

HSP 1 Score: 701.0 bits (1808), Expect = 5.4e-198
Identity = 365/375 (97.33%), Postives = 372/375 (99.20%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLM+LSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMILSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQ TIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQDTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDG+ SSSEDLKLPTQQTKEIEDNKMESLT
Sbjct: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGNCSSSEDLKLPTQQTKEIEDNKMESLT 360

Query: 361 IEAINSIELKNNIEQ 376
           IE+ NS++LKNNIEQ
Sbjct: 361 IESTNSVDLKNNIEQ 375

BLAST of Lsi01G014010 vs. NCBI nr
Match: XP_022143768.1 (WAT1-related protein At2g37460 [Momordica charantia])

HSP 1 Score: 675.6 bits (1742), Expect = 2.4e-190
Identity = 357/376 (94.95%), Postives = 367/376 (97.61%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MN Q+SQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNVQISQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIP+FAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPIFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKV+IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERAN+QQQRGEI
Sbjct: 121 MAWILRLEKVKIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANHQQQRGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGR+LGA VIIVGLYLVVWGKNKDG+ SSSE+LKLPT+QT+EIE NKMESLT
Sbjct: 301 FILGERLYFGRILGAGVIIVGLYLVVWGKNKDGN-SSSEELKLPTKQTREIEANKMESLT 360

Query: 361 IEAINSIELKNNIEQK 377
           IEA NS  L+N  EQK
Sbjct: 361 IEASNSSALQN--EQK 373

BLAST of Lsi01G014010 vs. NCBI nr
Match: XP_004142085.1 (WAT1-related protein At2g37460 [Cucumis sativus] >KGN54225.1 hypothetical protein Csa_018057 [Cucumis sativus])

HSP 1 Score: 667.9 bits (1722), Expect = 5.0e-188
Identity = 352/377 (93.37%), Postives = 364/377 (96.55%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYF+GMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFMGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGT+ATVGGAMIMTL+KGPI+ELFWVKERANN QQRGEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTVATVGGAMIMTLMKGPIVELFWVKERANNPQQRGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GN AVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNPAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNK-MESL 360
           FILGERLYFGRVLGA VIIVGLYLVVWGKNKD + SSSEDLKLPT+Q   +EDNK ME+L
Sbjct: 301 FILGERLYFGRVLGAVVIIVGLYLVVWGKNKDENCSSSEDLKLPTKQI--LEDNKTMETL 360

Query: 361 TIEAINSIELKNNIEQK 377
           TIE  + I+LKNN EQK
Sbjct: 361 TIEPNSCIDLKNNNEQK 375

BLAST of Lsi01G014010 vs. NCBI nr
Match: XP_008447554.1 (PREDICTED: WAT1-related protein At2g37460-like [Cucumis melo] >KAA0047346.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 662.5 bits (1708), Expect = 2.1e-186
Identity = 351/376 (93.35%), Postives = 360/376 (95.74%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MN QVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 1   MNSQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKVRIKSIRSQAK+VGT+ATVGGAMIMTL+KGPILELFWVKER  N QQ GEI
Sbjct: 121 MAWILRLEKVRIKSIRSQAKIVGTVATVGGAMIMTLMKGPILELFWVKERGTNPQQSGEI 180

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTV+ALVMER
Sbjct: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVVALVMER 240

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GN AVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 241 GNPAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGRVLGA VIIVGLYLVVWGKNKD + SS+EDLKLPT+QT  IEDNKMESLT
Sbjct: 301 FILGERLYFGRVLGAVVIIVGLYLVVWGKNKDENCSSNEDLKLPTKQT--IEDNKMESLT 360

Query: 361 IEAINSIELKNNIEQK 377
           IE    ++LK NIEQK
Sbjct: 361 IEPNICVDLK-NIEQK 373

BLAST of Lsi01G014010 vs. NCBI nr
Match: XP_022977879.1 (WAT1-related protein At2g37460-like [Cucurbita maxima])

HSP 1 Score: 644.8 bits (1662), Expect = 4.6e-181
Identity = 336/367 (91.55%), Postives = 353/367 (96.19%), Query Frame = 0

Query: 1   MNPQVSQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 60
           MNPQV QWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF
Sbjct: 35  MNPQVGQWCVSAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPF 94

Query: 61  ALIFDKKVRPKMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 120
           ALIFDKKVRPKMTIP+FAKLM+LSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV
Sbjct: 95  ALIFDKKVRPKMTIPIFAKLMILSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFV 154

Query: 121 MAWILRLEKVRIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEI 180
           MAWILRLEKV+IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVK+RA++ QQRG+I
Sbjct: 155 MAWILRLEKVKIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKQRADHAQQRGDI 214

Query: 181 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 240
           SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER
Sbjct: 215 SLQHTIKGSIMITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMER 274

Query: 241 GNAAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 300
           GNAAVWSI WGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS
Sbjct: 275 GNAAVWSIGWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSS 334

Query: 301 FILGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLT 360
           FILGERLYFGR+LGA VIIVGLYLVVWGKNKDGD S +EDLKLPT+QT E+  +   S +
Sbjct: 335 FILGERLYFGRILGAGVIIVGLYLVVWGKNKDGD-SLNEDLKLPTKQTTEMHKS---SSS 394

Query: 361 IEAINSI 368
           ++ +NS+
Sbjct: 395 VQELNSV 397

BLAST of Lsi01G014010 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.6e-126
Identity = 232/354 (65.54%), Postives = 282/354 (79.66%), Query Frame = 0

Query: 12  AKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPK 71
           A+PF ++V LQ GLAGMDILSKA LN+GMSNYVLVVYRHAVATIV+APFA  FDKKVRPK
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 72  MTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVR 131
           MT+ +F K+ +L LLEPVIDQNLY+LGMKYTTATFA AM N+LPAITFV+A+I  LE+V+
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 132 IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIM 191
           ++ IRS  K+VGT+ATVGGAMIMTL+KGP+L+LFW K    +        +   IKG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTK--GVSAHNTAGTDIHSAIKGAVL 192

Query: 192 ITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWG 251
           +TIGCFS+ACFMILQAITL+ YPAELSLTAWICL+GT EGT +ALVME+GN + W+I W 
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 252 TKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGR 311
           TKLL A YSGI CS LAYY+ G+VMK +GPVFVTAFSPL M+IVAIMS+ I  E++Y GR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 312 VLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTK-EIEDNKMESLTIEAI 365
           VLGA VI  GLYLV+WGK KD  Y+S+  L   + Q K E+  N  +++  E I
Sbjct: 313 VLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSGNGKDNVDHEVI 364

BLAST of Lsi01G014010 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 4.5e-110
Identity = 198/329 (60.18%), Postives = 250/329 (75.99%), Query Frame = 0

Query: 13  KPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPKM 72
           KPF  VV LQFG AG+ I++K ALNQGMS +VL  YRH VATI IAPFA   D+K+RPKM
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 73  TIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVRI 132
           T+ +F K+++L LLEP IDQNLY+ GMKYT+ATF AAM N+LPA  F+MAWI RLEKV +
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 133 KSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIMI 192
           K I SQAK++GTI TVGGAM+MT++KGP++ L W      +Q        Q   KG+ +I
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 193 TIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWGT 252
            IGC  WA F+ LQAITLK+YP ELSLTA+IC LG+ E T++AL +ERGN + W+I   +
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 253 KLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGRV 312
           KLLAAVY G+ CSG+ YY+QG++MK +GPVFVTAF+PLSMVIVAI+ S IL E ++ GR+
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 313 LGAAVIIVGLYLVVWGKNKDGDYSSSEDL 342
           LGA VI++GLY V+WGK+KD   SS  D+
Sbjct: 307 LGAIVIVLGLYSVLWGKSKDEPSSSFSDM 335

BLAST of Lsi01G014010 vs. TAIR 10
Match: AT2G37450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.7e-105
Identity = 201/353 (56.94%), Postives = 245/353 (69.41%), Query Frame = 0

Query: 12  AKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPK 71
           A PF  +V LQ G AGMDIL+K  LN+GMS YVL VYRH VAT+V+APFA  FD      
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD------ 66

Query: 72  MTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVR 131
                           PVI QNL+ LGMKYTTATFA A+ N LPA+TF++A I RLE V+
Sbjct: 67  ---------------NPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVK 126

Query: 132 IKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHTIKGSIM 191
            +SIRS AK+VGT+ TVGG M+MTL+KGP L+LFW K    + Q      +  +IKG+++
Sbjct: 127 FQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTK--GPSAQNTVGTDIHSSIKGAVL 186

Query: 192 ITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITWG 251
           +TIGCFS+ACFMILQAITLK YPAELSL  WICL+GT EG V+ALVME+GN +VW+I W 
Sbjct: 187 VTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWD 246

Query: 252 TKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFGR 311
           TKLL   YSGI CS L YYI G+VMK +GPVFVTAF PL M++VAIMSS I  E++Y GR
Sbjct: 247 TKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGR 306

Query: 312 VLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQTKEIEDNKMESLTIEAI 365
            LGA VI VGLYLV+WGK KD +Y S+  +     Q    +  +     IE++
Sbjct: 307 ALGATVICVGLYLVIWGKAKDYEYPSTPQIDDDLAQATTSKQKEQRRTVIESV 336

BLAST of Lsi01G014010 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.1e-95
Identity = 179/367 (48.77%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 11  SAKPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRP 70
           S+KP+FA++ LQFG AGM+I++K +LN GMS+YVLVVYRHA+AT VIAPFA  F++K +P
Sbjct: 15  SSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQP 74

Query: 71  KMTIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKV 130
           K+T  +F +L +L LL PVIDQN Y++G+KYT+ TF+ AM N+LPA+TF++A + R+E +
Sbjct: 75  KITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEML 134

Query: 131 RIKSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVK-------ERANNQQQRGEISLQ 190
            +K +  QAK+ GT+ TV GAM+MT+ KGPI+ELFW K         AN    +   S +
Sbjct: 135 DLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDK 194

Query: 191 HTIKGSIMITIGCFSWACFMILQAITLKAYPA-ELSLTAWICLLGTAEGTVLALVMERGN 250
             +KGSI++     +WA   +LQA  LK Y   +LSLT  IC +GT +   +  VME  N
Sbjct: 195 EFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEH-N 254

Query: 251 AAVWSITWGTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFI 310
            + W I W   LLAA YSGI  S ++YY+QG+VMK +GPVF TAFSPL MVIVA+M SF+
Sbjct: 255 PSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFV 314

Query: 311 LGERLYFGRVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQ--TKEIEDNKMESLT 368
           L E+++ G V+GA +I++GLY V+WGK K+   +  E  K+ +    T+++E N  +   
Sbjct: 315 LAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKI 374

BLAST of Lsi01G014010 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 345.9 bits (886), Expect = 4.1e-95
Identity = 175/353 (49.58%), Postives = 255/353 (72.24%), Query Frame = 0

Query: 13  KPFFAVVFLQFGLAGMDILSKAALNQGMSNYVLVVYRHAVATIVIAPFALIFDKKVRPKM 72
           +P+  ++FLQFG AG  I+  A LNQG + YV++VYR+ VA +V+APFALIF++KVRPKM
Sbjct: 12  RPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRPKM 71

Query: 73  TIPVFAKLMVLSLLEPVIDQNLYFLGMKYTTATFAAAMCNILPAITFVMAWILRLEKVRI 132
           T+ V  K+M L  LEPV+DQ   +LGM  T+AT+ +A+ NILP++TF++AWILR+EKV I
Sbjct: 72  TLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKVNI 131

Query: 133 KSIRSQAKLVGTIATVGGAMIMTLIKGPILELFWVKERANNQQQRGEISLQHT--IKGSI 192
             +RS+AK++GT+  +GGA++MTL KGP++ L W     + Q      S  H   + G++
Sbjct: 132 AEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVGTL 191

Query: 193 MITIGCFSWACFMILQAITLKAYPAELSLTAWICLLGTAEGTVLALVMERGNAAVWSITW 252
           +I +GC +W+ F +LQ+IT+K YPA+LSL+A ICL G  +   +ALV+ER + + W++ W
Sbjct: 192 LILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVER-HPSGWAVGW 251

Query: 253 GTKLLAAVYSGIFCSGLAYYIQGLVMKDKGPVFVTAFSPLSMVIVAIMSSFILGERLYFG 312
             +L A +Y+GI  SG+ YY+QG+VMK +GPVFVTAF+PL M++VA+++SFIL E+++FG
Sbjct: 252 DARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHFG 311

Query: 313 RVLGAAVIIVGLYLVVWGKNKDGDYSSSEDLKLPTQQ-----TKEIEDNKMES 359
            V+G AVI  GLY+VVWGK KD + S  + L+  + Q     TK  +DNK+ S
Sbjct: 312 CVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDDNKLVS 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUS13.7e-12565.54WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
O806386.3e-10960.18WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
F4IQX18.0e-10456.94WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2... [more]
Q9FL414.4e-9448.77WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q9SUF15.8e-9449.58WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1CRJ21.2e-19094.95WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111013601 PE=3 SV=1[more]
A0A0A0KX582.4e-18893.37WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_4G293840 PE=3 SV=1[more]
A0A5A7TZF91.0e-18693.35WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908... [more]
A0A1S3BIL91.0e-18693.35WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103489968 PE=3 SV=1[more]
A0A6J1ISL32.2e-18191.55WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478038 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883137.15.4e-19897.33WAT1-related protein At2g37460 [Benincasa hispida][more]
XP_022143768.12.4e-19094.95WAT1-related protein At2g37460 [Momordica charantia][more]
XP_004142085.15.0e-18893.37WAT1-related protein At2g37460 [Cucumis sativus] >KGN54225.1 hypothetical protei... [more]
XP_008447554.12.1e-18693.35PREDICTED: WAT1-related protein At2g37460-like [Cucumis melo] >KAA0047346.1 WAT1... [more]
XP_022977879.14.6e-18191.55WAT1-related protein At2g37460-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G37460.12.6e-12665.54nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.14.5e-11060.18nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37450.25.7e-10556.94nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.13.1e-9548.77nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.14.1e-9549.58nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 366..376
NoneNo IPR availablePANTHERPTHR31218:SF330WAT1-RELATED PROTEINcoord: 9..343
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 233..331
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 48..156
IPR000620EamA domainPFAMPF00892EamAcoord: 187..326
e-value: 6.2E-14
score: 52.3
coord: 23..135
e-value: 1.9E-11
score: 44.3
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G014010.1Lsi01G014010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity