Lcy10g004850 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g004850
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionprotein MIZU-KUSSEI 1-like
LocationChr10: 12877854 .. 12878603 (-)
RNA-Seq ExpressionLcy10g004850
SyntenyLcy10g004850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGAAACAACGCCTTGAGCTGGACAGTCTCACCAGATCCACCAGTACAAGCAGCACTACCAAAATGCTACCTTCTTCCACACAAACAGCCATCGTTCCCAAGCCCACCTCAACCACAACCACGTCGTCCCACAAATCCTCCCTCCTCCACTCCCTCTTCCACTTCCTCCTCCACCGCCGCAACTGGCTCTCCAACATAATCCCCGCCACCACCACCACCCTCTCCCTCTCTCTACGCCGCAAGCTCACCGGCACCCTCTTCGGCCACCGCCACGGCCATGTCACCTTCTCCCTCCAGCTCGACCCACGTGCCGAACCCCTCACGCTGCTCCACCTCGACATGTCGACCACCACGCTCCTCAAGGAGATGTCGTCCGGTCTCCTCCGCATAGTCCTCGAGTCCCACAAACAACCTGCCCGAGCCCGCCCCACTAAGCTCCTCAAACAGCCCACCTGGACCATGTACTGCAACGGAACGGAAACTGGCCGAGCCCTCTCACGCACGTGCGAGGCCTACGACCGCCACGTCTTGAACACCATTCGGACCGTGTCCGTTGGAGCCGGCGTGATTCCGGTCCTCCACGACGGAAGCAAAGGCGGCGCCTGCTCGGAACTCGGTGAGTTGGTGTATATGAGAGCCGAGTTTGAACGAGTCGTTGGAAGCGCTGACTCGGAAGCTTTGTTCATGATTAACCCCGATGGCAATGGCGGCTCTGAACTCAGTATATTTTTGCTTAGAATATGA

mRNA sequence

ATGAAAATGAAACAACGCCTTGAGCTGGACAGTCTCACCAGATCCACCAGTACAAGCAGCACTACCAAAATGCTACCTTCTTCCACACAAACAGCCATCGTTCCCAAGCCCACCTCAACCACAACCACGTCGTCCCACAAATCCTCCCTCCTCCACTCCCTCTTCCACTTCCTCCTCCACCGCCGCAACTGGCTCTCCAACATAATCCCCGCCACCACCACCACCCTCTCCCTCTCTCTACGCCGCAAGCTCACCGGCACCCTCTTCGGCCACCGCCACGGCCATGTCACCTTCTCCCTCCAGCTCGACCCACGTGCCGAACCCCTCACGCTGCTCCACCTCGACATGTCGACCACCACGCTCCTCAAGGAGATGTCGTCCGGTCTCCTCCGCATAGTCCTCGAGTCCCACAAACAACCTGCCCGAGCCCGCCCCACTAAGCTCCTCAAACAGCCCACCTGGACCATGTACTGCAACGGAACGGAAACTGGCCGAGCCCTCTCACGCACGTGCGAGGCCTACGACCGCCACGTCTTGAACACCATTCGGACCGTGTCCGTTGGAGCCGGCGTGATTCCGGTCCTCCACGACGGAAGCAAAGGCGGCGCCTGCTCGGAACTCGGTGAGTTGGTGTATATGAGAGCCGAGTTTGAACGAGTCGTTGGAAGCGCTGACTCGGAAGCTTTGTTCATGATTAACCCCGATGGCAATGGCGGCTCTGAACTCAGTATATTTTTGCTTAGAATATGA

Coding sequence (CDS)

ATGAAAATGAAACAACGCCTTGAGCTGGACAGTCTCACCAGATCCACCAGTACAAGCAGCACTACCAAAATGCTACCTTCTTCCACACAAACAGCCATCGTTCCCAAGCCCACCTCAACCACAACCACGTCGTCCCACAAATCCTCCCTCCTCCACTCCCTCTTCCACTTCCTCCTCCACCGCCGCAACTGGCTCTCCAACATAATCCCCGCCACCACCACCACCCTCTCCCTCTCTCTACGCCGCAAGCTCACCGGCACCCTCTTCGGCCACCGCCACGGCCATGTCACCTTCTCCCTCCAGCTCGACCCACGTGCCGAACCCCTCACGCTGCTCCACCTCGACATGTCGACCACCACGCTCCTCAAGGAGATGTCGTCCGGTCTCCTCCGCATAGTCCTCGAGTCCCACAAACAACCTGCCCGAGCCCGCCCCACTAAGCTCCTCAAACAGCCCACCTGGACCATGTACTGCAACGGAACGGAAACTGGCCGAGCCCTCTCACGCACGTGCGAGGCCTACGACCGCCACGTCTTGAACACCATTCGGACCGTGTCCGTTGGAGCCGGCGTGATTCCGGTCCTCCACGACGGAAGCAAAGGCGGCGCCTGCTCGGAACTCGGTGAGTTGGTGTATATGAGAGCCGAGTTTGAACGAGTCGTTGGAAGCGCTGACTCGGAAGCTTTGTTCATGATTAACCCCGATGGCAATGGCGGCTCTGAACTCAGTATATTTTTGCTTAGAATATGA

Protein sequence

MKMKQRLELDSLTRSTSTSSTTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADSEALFMINPDGNGGSELSIFLLRI
Homology
BLAST of Lcy10g004850 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.5e-47
Identity = 125/271 (46.13%), Postives = 164/271 (60.52%), Query Frame = 0

Query: 11  SLTRSTSTSSTTKMLPSSTQTAIVP------KPTSTTTTSSHKSSLLHSLFHFLLH---- 70
           S  RS S+S+    +P   +  +VP       P S+++++SH     H  F  LL     
Sbjct: 30  SHVRSPSSSALIPSIPEH-ELFLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFIN 89

Query: 71  -----------------RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLD 130
                              + +SN + +  T  S SL R++TGTL+GH+ GHVTFS+Q +
Sbjct: 90  IINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYN 149

Query: 131 PRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTET 190
            R++P+ LL L MST TL+KEMSSGL+RI LE  K+      TKL ++P WTMYCNG + 
Sbjct: 150 QRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR--HRSGTKLFQEPKWTMYCNGRKC 209

Query: 191 GRALSR--TCEAYDRHVLNTIRTVSVGAGVIP---VLHDGSKGGACSELGELVYMRAEFE 250
           G A+SR   C   D  VLNT+  V+VGAGVIP    + D S  G+ +ELGEL+YMR +FE
Sbjct: 210 GYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFE 269

BLAST of Lcy10g004850 vs. ExPASy TrEMBL
Match: A0A6J1ICS4 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471899 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.2e-97
Identity = 205/263 (77.95%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKMLPSST-----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ LTRST   STSSTTKML SS+     Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLTRSTSTASTSSTTKMLQSSSTFNPPQMAIVPNPPSATSSHKPHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FL HRRN LS         LSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLFHRRNSLS---------LSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 254

BLAST of Lcy10g004850 vs. ExPASy TrEMBL
Match: A0A6J1G1R9 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449855 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 6.5e-97
Identity = 205/263 (77.95%), Postives = 219/263 (83.27%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKML-PSST----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ L RST   STSSTTKML PSST    Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLARSTSTASTSSTTKMLQPSSTFNPPQMAIVPNPPSATSSHKSHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FL HRRN LS      + TL L+LRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLFHRRNSLS-----LSLTLGLTLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 258

BLAST of Lcy10g004850 vs. ExPASy TrEMBL
Match: A0A0A0LHM1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822475 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 5.5e-88
Identity = 186/250 (74.40%), Postives = 201/250 (80.40%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRSTSTSSTTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLHSLFHFLLH 60
           M+ ++ LEL SL+RST          SS  TAIV KP STT++ S  SSLLHSLF F LH
Sbjct: 1   MRQRRHLELSSLSRST----------SSADTAIVLKPCSTTSSHS-PSSLLHSLFRFFLH 60

Query: 61  RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTT 120
           RRN LS I          +LRRKLTGTLFGHRHGHVTFSLQLDPR EPLTLLHL +STT 
Sbjct: 61  RRNALSLI----------TLRRKLTGTLFGHRHGHVTFSLQLDPRTEPLTLLHLHISTTA 120

Query: 121 LLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLN 180
           LLKEMSSG+LRI L SHK P RARPTKLL+ P+WTMYCNGTE+G ALSRTCEAYDRHVLN
Sbjct: 121 LLKEMSSGVLRIALHSHKLPGRARPTKLLQHPSWTMYCNGTESGLALSRTCEAYDRHVLN 180

Query: 181 TIRTVSVGAGVIPVLHDGSK-GGACSELGELVYMRAEFERVVGSADSEALFMINPDGNGG 240
           TIR+VSVGAGVIPVLHDG+K  G CSELG LVYMRAEFERVVGSADSEALFMINPDGN  
Sbjct: 181 TIRSVSVGAGVIPVLHDGTKAAGGCSELGALVYMRAEFERVVGSADSEALFMINPDGNAA 229

Query: 241 SELSIFLLRI 250
           SEL+IFLLRI
Sbjct: 241 SELTIFLLRI 229

BLAST of Lcy10g004850 vs. ExPASy TrEMBL
Match: A0A5A7UF10 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007250 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.2e-87
Identity = 186/250 (74.40%), Postives = 201/250 (80.40%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRSTSTSSTTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLHSLFHFLLH 60
           M+ ++ LEL SL+RST          SST TAIV KP ST++ S   SSLLHSLF F LH
Sbjct: 1   MRQRRHLELSSLSRST----------SSTDTAIVLKPCSTSSHS--PSSLLHSLFRFFLH 60

Query: 61  RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTT 120
           RRN LS I          +LRRKLTGTLFGHRHGHVTFSLQLDPR EPLTLLHL +STT 
Sbjct: 61  RRNALSLI----------TLRRKLTGTLFGHRHGHVTFSLQLDPRTEPLTLLHLHISTTA 120

Query: 121 LLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLN 180
           LLKEMSSG+LRI L SHK P RARPTKLL+ P+WTMYCNGTE+G ALSRTCEAYDRHVLN
Sbjct: 121 LLKEMSSGVLRIALHSHKLPGRARPTKLLQHPSWTMYCNGTESGLALSRTCEAYDRHVLN 180

Query: 181 TIRTVSVGAGVIPVLHDGSK-GGACSELGELVYMRAEFERVVGSADSEALFMINPDGNGG 240
           TIR+VSVGAGVIPVLHDG+K  G CSELG LVYMRAEFERVVGSADSEALFMINPDGN  
Sbjct: 181 TIRSVSVGAGVIPVLHDGTKAAGGCSELGALVYMRAEFERVVGSADSEALFMINPDGNAT 228

Query: 241 SELSIFLLRI 250
           SEL+IFLLRI
Sbjct: 241 SELTIFLLRI 228

BLAST of Lcy10g004850 vs. ExPASy TrEMBL
Match: A0A1S3B7Q2 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103486937 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.2e-87
Identity = 186/250 (74.40%), Postives = 201/250 (80.40%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRSTSTSSTTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLHSLFHFLLH 60
           M+ ++ LEL SL+RST          SST TAIV KP ST++ S   SSLLHSLF F LH
Sbjct: 1   MRQRRHLELSSLSRST----------SSTDTAIVLKPCSTSSHS--PSSLLHSLFRFFLH 60

Query: 61  RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTT 120
           RRN LS I          +LRRKLTGTLFGHRHGHVTFSLQLDPR EPLTLLHL +STT 
Sbjct: 61  RRNALSLI----------TLRRKLTGTLFGHRHGHVTFSLQLDPRTEPLTLLHLHISTTA 120

Query: 121 LLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLN 180
           LLKEMSSG+LRI L SHK P RARPTKLL+ P+WTMYCNGTE+G ALSRTCEAYDRHVLN
Sbjct: 121 LLKEMSSGVLRIALHSHKLPGRARPTKLLQHPSWTMYCNGTESGLALSRTCEAYDRHVLN 180

Query: 181 TIRTVSVGAGVIPVLHDGSK-GGACSELGELVYMRAEFERVVGSADSEALFMINPDGNGG 240
           TIR+VSVGAGVIPVLHDG+K  G CSELG LVYMRAEFERVVGSADSEALFMINPDGN  
Sbjct: 181 TIRSVSVGAGVIPVLHDGTKAAGGCSELGALVYMRAEFERVVGSADSEALFMINPDGNAT 228

Query: 241 SELSIFLLRI 250
           SEL+IFLLRI
Sbjct: 241 SELTIFLLRI 228

BLAST of Lcy10g004850 vs. NCBI nr
Match: KAG6596966.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028440.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 207/263 (78.71%), Postives = 220/263 (83.65%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKML-PSST----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ LTRST   STSSTTKML PSST    Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLTRSTSTASTSSTTKMLQPSSTFNPPQMAIVPNPPSATSSHKSHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FL HRRN LS      T TL L+LRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLFHRRNSLS-----LTRTLGLTLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 258

BLAST of Lcy10g004850 vs. NCBI nr
Match: XP_023540797.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 207/263 (78.71%), Postives = 221/263 (84.03%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKML-PSST----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ LTRST   STSSTTKML PSST    Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLTRSTSTASTSSTTKMLQPSSTFNPPQMAIVPNPPSATSSHKSHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FLLHRRN LS      + TL L+LRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLLHRRNSLS-----LSLTLGLTLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 258

BLAST of Lcy10g004850 vs. NCBI nr
Match: XP_022973343.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 365.2 bits (936), Expect = 4.6e-97
Identity = 205/263 (77.95%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKMLPSST-----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ LTRST   STSSTTKML SS+     Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLTRSTSTASTSSTTKMLQSSSTFNPPQMAIVPNPPSATSSHKPHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FL HRRN LS         LSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLFHRRNSLS---------LSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 254

BLAST of Lcy10g004850 vs. NCBI nr
Match: XP_022945715.1 (protein MIZU-KUSSEI 1-like [Cucurbita moschata])

HSP 1 Score: 363.6 bits (932), Expect = 1.3e-96
Identity = 205/263 (77.95%), Postives = 219/263 (83.27%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRST---STSSTTKML-PSST----QTAIVPKPTSTTTT------SSH 60
           MKM+  LEL+ L RST   STSSTTKML PSST    Q AIVP P S T++      S+ 
Sbjct: 1   MKMQHHLELNPLARSTSTASTSSTTKMLQPSSTFNPPQMAIVPNPPSATSSHKSHSLSNR 60

Query: 61  KSSLLHSLFHFLLHRRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRA 120
            SSLLHSLF FL HRRN LS      + TL L+LRRKLTGTLFGHRHGHVTFSLQLDPRA
Sbjct: 61  ASSLLHSLFQFLFHRRNSLS-----LSLTLGLTLRRKLTGTLFGHRHGHVTFSLQLDPRA 120

Query: 121 EPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRA 180
           +PL+LLHLD+STT LLKEMSSGLLRI LE+HK P RA+P+KLLKQP WTMYCNG ETG A
Sbjct: 121 QPLSLLHLDISTTALLKEMSSGLLRIALEAHKLPGRAQPSKLLKQPKWTMYCNGRETGLA 180

Query: 181 LSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADS 240
           LSRTCE YDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGS+DS
Sbjct: 181 LSRTCEPYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSSDS 240

Query: 241 EALFMINPDGNGGSELSIFLLRI 250
           EALFMINPDGN GSELSIFLLRI
Sbjct: 241 EALFMINPDGNCGSELSIFLLRI 258

BLAST of Lcy10g004850 vs. NCBI nr
Match: XP_038903241.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 344.7 bits (883), Expect = 6.5e-91
Identity = 189/250 (75.60%), Postives = 204/250 (81.60%), Query Frame = 0

Query: 1   MKMKQRLELDSLTRSTSTSSTTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLHSLFHFLLH 60
           MK  + LEL SL RST          SST TAIVPKP+ +TT+S   SSLLHSLFHFLLH
Sbjct: 1   MKQHRHLELSSLGRST----------SSTDTAIVPKPSYSTTSSHRPSSLLHSLFHFLLH 60

Query: 61  RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTT 120
           RRN LS +          +L RKLTGTLFGHRHGHVTFSLQLDPR+EPLTLLHL +STTT
Sbjct: 61  RRNALSLV----------TLPRKLTGTLFGHRHGHVTFSLQLDPRSEPLTLLHLHISTTT 120

Query: 121 LLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLN 180
           LL EMSSG+LRI L SHK P RARPTKLL+ P+WTMYCNGTE+G ALSRTCEAYDRHVLN
Sbjct: 121 LLTEMSSGVLRIALHSHKLPGRARPTKLLQHPSWTMYCNGTESGLALSRTCEAYDRHVLN 180

Query: 181 TIRTVSVGAGVIPVLHDGSKGGA-CSELGELVYMRAEFERVVGSADSEALFMINPDGNGG 240
           TIR+VSVGAGVIPVLHDG+K GA CSELG LVYMRAEFERVVGSADSEALFMINPDGN  
Sbjct: 181 TIRSVSVGAGVIPVLHDGTKAGAGCSELGALVYMRAEFERVVGSADSEALFMINPDGNAA 230

Query: 241 SELSIFLLRI 250
           SEL+IFLLRI
Sbjct: 241 SELTIFLLRI 230

BLAST of Lcy10g004850 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 189.9 bits (481), Expect = 2.5e-48
Identity = 125/271 (46.13%), Postives = 164/271 (60.52%), Query Frame = 0

Query: 11  SLTRSTSTSSTTKMLPSSTQTAIVP------KPTSTTTTSSHKSSLLHSLFHFLLH---- 70
           S  RS S+S+    +P   +  +VP       P S+++++SH     H  F  LL     
Sbjct: 30  SHVRSPSSSALIPSIPEH-ELFLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFIN 89

Query: 71  -----------------RRNWLSNIIPATTTTLSLSLRRKLTGTLFGHRHGHVTFSLQLD 130
                              + +SN + +  T  S SL R++TGTL+GH+ GHVTFS+Q +
Sbjct: 90  IINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYN 149

Query: 131 PRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPARARPTKLLKQPTWTMYCNGTET 190
            R++P+ LL L MST TL+KEMSSGL+RI LE  K+      TKL ++P WTMYCNG + 
Sbjct: 150 QRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR--HRSGTKLFQEPKWTMYCNGRKC 209

Query: 191 GRALSR--TCEAYDRHVLNTIRTVSVGAGVIP---VLHDGSKGGACSELGELVYMRAEFE 250
           G A+SR   C   D  VLNT+  V+VGAGVIP    + D S  G+ +ELGEL+YMR +FE
Sbjct: 210 GYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFE 269

BLAST of Lcy10g004850 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-36
Identity = 102/269 (37.92%), Postives = 149/269 (55.39%), Query Frame = 0

Query: 15  STSTSSTTKMLPSSTQTAIVPKPTSTTT----------TSSHKSSLLHSLFHFLLHRRNW 74
           +TS+SS++  +P S+ T   P PTSTTT           SS K     ++F  L   R  
Sbjct: 9   ATSSSSSSSSIPPSSPT---PSPTSTTTPPRQHPLLQPPSSKKKKNRTNVFRVL---RTV 68

Query: 75  LSNIIPATTTTLSLSL---------------RRKLTGTLFGHRHGHVTFSLQLDPRAEPL 134
             +    TT +++  +                 ++TGTLFG+R G V+ S+Q +P+  P 
Sbjct: 69  FRSFPIFTTPSVACKIPVIHPGLGLPDPHHNTSRITGTLFGYRKGRVSLSIQENPKCLPS 128

Query: 135 TLLHLDMSTTTLLKEMSSGLLRIVLESHKQP-------ARARPTKLLKQPTWTMYCNGTE 194
            ++ L M TTTL KE+S+G++RI LE+ KQP          + T +L++P WTMYC G +
Sbjct: 129 LVVELAMQTTTLQKELSTGMVRIALETEKQPRADNNNSKTEKKTDILEEPLWTMYCKGEK 188

Query: 195 TGRALSRTCEAYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSE--LGELVYMRAEFERV 250
           TG  + R     D +V+  +R VS+GAGV+P       G + SE   GE+ YMRA FERV
Sbjct: 189 TGYGVKREATEEDLNVMELLRPVSMGAGVLP-------GNSESEGPDGEMAYMRAYFERV 248

BLAST of Lcy10g004850 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 149.4 bits (376), Expect = 3.7e-36
Identity = 76/172 (44.19%), Postives = 109/172 (63.37%), Query Frame = 0

Query: 83  KLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPAR 142
           ++TGTLFG+R   V  ++Q +PR+ P+ LL L + T  LL+++  GL+RI LE  K+P  
Sbjct: 92  RVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGVGLVRIALECEKKP-- 151

Query: 143 ARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLNTIRTVSVGAGVIPV-----LHD 202
           +  TK++ +P W +YCNG ++G  + R     D  V+  +  VS+GAGV+PV        
Sbjct: 152 SEKTKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGAGVLPVSSGAITEQ 211

Query: 203 GSKGGACSELGELVYMRAEFERVVGSADSEALFMINPDGNGGSELSIFLLRI 250
              GG   + G+L YMRA FERV+GS DSE  +M+NPDGN G ELSIF +R+
Sbjct: 212 SGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPELSIFFVRV 261

BLAST of Lcy10g004850 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-35
Identity = 78/172 (45.35%), Postives = 111/172 (64.53%), Query Frame = 0

Query: 83  KLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLESHKQPAR 142
           ++ GTLFG+R GHV F++Q DP   P  L+ L   T+ L++EM+SGL+RI LE+      
Sbjct: 97  RVVGTLFGNRRGHVYFAVQDDPTRLPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 156

Query: 143 ARPTKLLKQPTWTMYCNGTETGRALSRTCEAYDRHVLNTIRTVSVGAGVIP----VLHDG 202
           ++  KLL++ TW  YCNG + G A  + C   +  VL  +  +++GAGV+P     + + 
Sbjct: 157 SK-KKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEE 216

Query: 203 SKGGACSELGELVYMRAEFERVVGSADSEALFMINPD-GNGGSELSIFLLRI 250
             G   SE GEL+YMRA FERVVGS DSEA +M+NPD  +GG ELS++ LR+
Sbjct: 217 GNGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of Lcy10g004850 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-34
Identity = 98/257 (38.13%), Postives = 140/257 (54.47%), Query Frame = 0

Query: 20  STTKMLPSSTQTAIVPKPTSTTTTSSHKSSLLH--SLFHFLLHR-RNWLSNIIPATTTTL 79
           S+ K LP    T    KP+S++++SS  + + H   L  F + R R+ ++ +  A     
Sbjct: 28  SSHKPLPHHNNT----KPSSSSSSSS-SNVITHKKKLQSFAVSRLRSVIATLSRARPGNQ 87

Query: 80  SLSLRRKLTGTLFGHRHGHVTFSLQLDPRAEPLTLLHLDMSTTTLLKEMSSGLLRIVLES 139
           +  L  ++ GTLFG R GHV FS+Q DP + P  L+ L    + L+KEM+SGL+RI LE 
Sbjct: 88  NSGLGSRVVGTLFGSRRGHVHFSIQKDPNSPPAFLIELATPISGLVKEMASGLVRIALEC 147

Query: 140 HK------------------------QPARARPTKLLKQPTWTMYCNGTETGRALSRTCE 199
            K                            A   +L+++P W  YCNG + G A  R C 
Sbjct: 148 DKGKEEEEGEEKNGTLRHGGGDKTKTTTTAAVSRRLVEEPMWRTYCNGKKCGFATRRECG 207

Query: 200 AYDRHVLNTIRTVSVGAGVIPVLHDGSKGGACSELGELVYMRAEFERVVGSADSEALFMI 250
             ++ VL  +  VS+GAGV+P   +   GG     G+++YMRA+FER+VGS DSEA +M+
Sbjct: 208 EKEKKVLKALEMVSMGAGVLPETEEIGGGGGGG--GDIMYMRAKFERIVGSRDSEAFYMM 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222273.5e-4746.13Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ICS42.2e-9777.95protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471899 PE=4 SV=1[more]
A0A6J1G1R96.5e-9777.95protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449855 PE=4 SV... [more]
A0A0A0LHM15.5e-8874.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822475 PE=4 SV=1[more]
A0A5A7UF101.2e-8774.40Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35... [more]
A0A1S3B7Q21.2e-8774.40protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103486937 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG6596966.11.2e-9778.71Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG70284... [more]
XP_023540797.11.2e-9778.71protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo][more]
XP_022973343.14.6e-9777.95protein MIZU-KUSSEI 1-like [Cucurbita maxima][more]
XP_022945715.11.3e-9677.95protein MIZU-KUSSEI 1-like [Cucurbita moschata][more]
XP_038903241.16.5e-9175.60protein MIZU-KUSSEI 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G41660.12.5e-4846.13Protein of unknown function, DUF617 [more]
AT4G39610.11.7e-3637.92Protein of unknown function, DUF617 [more]
AT5G06990.13.7e-3644.19Protein of unknown function, DUF617 [more]
AT3G25640.11.1e-3545.35Protein of unknown function, DUF617 [more]
AT5G23100.12.0e-3438.13Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 85..248
e-value: 8.1E-59
score: 198.4
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 85..249
e-value: 9.0E-60
score: 199.2
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 14..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..45
NoneNo IPR availablePANTHERPTHR31696:SF71PROTEIN MIZU-KUSSEI 1coord: 14..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g004850.1Lcy10g004850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0010274 hydrotropism
cellular_component GO:0032541 cortical endoplasmic reticulum
cellular_component GO:0005789 endoplasmic reticulum membrane