Lcy03g017680 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g017680 vs. ExPASy Swiss-Prot
Match: Q8CD15 (Ribosomal oxygenase 2 OS=Mus musculus OX=10090 GN=Riox2 PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 5.3e-17
Identity = 62/156 (39.74%), Postives = 83/156 (53.21%), Query Frame = 0

Query: 427 HFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGS 486
           HFL+L      + A     TI     +   +++  I   L   FG   VG+N+Y+TP GS
Sbjct: 120 HFLQLRKDFDQKRA-----TIQFHQPQRYKDELWRIQEKLECYFGS-LVGSNVYMTPAGS 179

Query: 487 QGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAVG---RQ 546
           QGL  HYDD  VF+ QL G+K W ++SP    +P    AHE+     VES   +G     
Sbjct: 180 QGLPPHYDDVEVFILQLEGTKHWRLYSPT---VPL---AHEY----SVESEDRIGTPTHD 239

Query: 547 FLLREGDVLYIPRGFLHEARTENDGPDG--SSLHLT 578
           FLL+ GD+LY PRG +H+A T    P G   S+HLT
Sbjct: 240 FLLKPGDLLYFPRGTIHQAET----PSGLAYSIHLT 255

BLAST of Lcy03g017680 vs. ExPASy Swiss-Prot
Match: B4I100 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila sechellia OX=7238 GN=GM12339 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 6.9e-17
Identity = 54/144 (37.50%), Postives = 73/144 (50.69%), Query Frame = 0

Query: 441 FNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGSQGLARHYDDHCVFV 500
           ++EG +I L        ++  +   L   F    VGANMYLTPP SQG A HYDD   FV
Sbjct: 303 YSEGCSIRLLHASAYLTRLREVCTVLQEFF-HCKVGANMYLTPPNSQGFAPHYDDIEAFV 362

Query: 501 CQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVE-SPLAVGRQFL---LREGDVLYIPR 560
            Q+ G K+W ++ PPK       +A    R S    +   +G+  +   L  GDVLY PR
Sbjct: 363 IQVEGRKRWLLYDPPK-------EADHLARISSGNYNQEQLGKPIIDEVLSAGDVLYFPR 422

Query: 561 GFLHEARTENDGPDGSSLHLTFGI 581
           G +H+A TE       SLH+T  +
Sbjct: 423 GTVHQAITEE---QQHSLHITLSV 435

BLAST of Lcy03g017680 vs. ExPASy Swiss-Prot
Match: B4L6Q5 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila mojavensis OX=7230 GN=GI16410 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.2e-16
Identity = 50/140 (35.71%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 441 FNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGSQGLARHYDDHCVFV 500
           + +G +I +        K+  + +T+   F    VGAN+YLTPP SQG A HYDD   FV
Sbjct: 560 YGDGCSIRILNPSTYLPKLRQLCSTMQEFF-HCLVGANVYLTPPNSQGFAPHYDDIEAFV 619

Query: 501 CQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAVGRQFLLREGDVLYIPRGFLH 560
            Q+ G K+W +++PP         +    +  E+  PL      +L  GD+LY PRG +H
Sbjct: 620 IQVEGRKRWRLYAPPHQSDVLARTSSGNYKQEELGQPLF---DAVLEAGDILYFPRGTVH 679

Query: 561 EARTENDGPDGSSLHLTFGI 581
           +A TE   P   SLH+T  +
Sbjct: 680 QAVTE---PKQHSLHITLSV 692

BLAST of Lcy03g017680 vs. ExPASy Swiss-Prot
Match: B4GUZ2 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila persimilis OX=7234 GN=GL12933 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.6e-16
Identity = 47/103 (45.63%), Postives = 65/103 (63.11%), Query Frame = 0

Query: 475 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEV 534
           VGAN+YLTPP SQG A HYDD   FV Q+ G K+W +++P K  LPR  ++      +E+
Sbjct: 370 VGANVYLTPPESQGFAPHYDDIEAFVLQVEGKKRWRIYAPTKE-LPR--ESSGNLSQTEL 429

Query: 535 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLT 578
             P+      +L+ GD+LY PRG++H+A TE    D  SLH+T
Sbjct: 430 GDPI---MDIVLKPGDLLYFPRGWIHQAITEK---DSHSLHIT 463

BLAST of Lcy03g017680 vs. ExPASy Swiss-Prot
Match: B3MSI4 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila ananassae OX=7217 GN=GF20792 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-16
Identity = 77/252 (30.56%), Postives = 112/252 (44.44%), Query Frame = 0

Query: 341 SSFLGSVASIETIHSFISTMLGRLV---SCNPIASDEIDIHNFLEE--VRNELGFPLIYQ 400
           SS  G  A  +  H   S   GR +     NP+  DE    +F E+   + +   P  + 
Sbjct: 383 SSKSGDKAKSKDYHKVNSIEEGRRILQWILNPVQPDEF-FGSFWEKNACQVQRQTPKYFA 442

Query: 401 QDIRVLRTDECLKREIHYFQNNFEPCCIKGPHFLKLNDALKCE-----EAFNEGYTIALR 460
           + I     DE L R    F  N +    K      LN   +        ++ +G +I + 
Sbjct: 443 ELISFEMIDEMLIRHHLEFTTNIDVTTYKDGVRQTLNPEGRAMPPAVWSSYADGCSIRIL 502

Query: 461 GMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWT 520
                   +  + + L   F    VGAN+YLTPP SQG A HYDD   FV Q+ G K+W 
Sbjct: 503 NPSTYLAGLRQVCSMLQEFF-HCLVGANVYLTPPNSQGFAPHYDDIEAFVLQVEGRKRWR 562

Query: 521 VFSP--PKMWLPRLYDAHEFPRCSEVESPLAVGRQFLLREGDVLYIPRGFLHEARTENDG 580
           ++ P  P   L R + +  F +  E++ P+      +L  GDVLY PRG +H+A TE   
Sbjct: 563 LYMPVKPTDMLAR-HSSGNFDQ-GELDEPIF---DEVLEAGDVLYFPRGTVHQAITEK-- 622

BLAST of Lcy03g017680 vs. ExPASy TrEMBL
Match: A0A6J1JJ52 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucurbita maxima OX=3661 GN=LOC111484915 PE=3 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 670/781 (85.79%), Postives = 714/781 (91.42%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS+TSTSKTKRKR+SNS  +FD NTT SLLLAAVS+P NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSRTSTSKTKRKRRSNS--DFDVNTTFSLLLAAVSTPRNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSER GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALIS LAC KRRVALAACNAV+DLSTTSFGR+ LVE SAIE LISAFLQVSASS
Sbjct: 121 ADGVVRALISTLACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSV IGT+Y ENSAILK GF+GDELSI IL+AAIVL+NTC+FKHLEKIP YY + FL F
Sbjct: 181 AMSVFIGTVYIENSAILKAGFEGDELSISILSAAIVLLNTCYFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           LK QWIEV SKMMQIN++ECSWQ QF+ +SNISTNDLAV IFRLSISTDQVSR+ PVKEV
Sbjct: 241 LKKQWIEVCSKMMQINKTECSWQEQFE-ISNISTNDLAVCIFRLSISTDQVSRIFPVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           K LLGLSGSNFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SI+T  SFIS+M
Sbjct: 301 KALLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIKTNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+FQ NFEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFQKNFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC KGPHFLKLNDA KCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSP KM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPTKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC-- 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YC  
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWN 600

Query: 601 WNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
           WNDN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+FLPSD N+R DLNQKTI
Sbjct: 601 WNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNRPDLNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVDGKES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQWIMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NAVECYK+LLER+R VRSQY TGMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYTTGMISL 778

BLAST of Lcy03g017680 vs. ExPASy TrEMBL
Match: A0A6J1E073 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111429646 PE=3 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 671/781 (85.92%), Postives = 711/781 (91.04%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS TSTSKTKRKR+SNS  +FD NTT SLLLAAVS+P NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSCTSTSKTKRKRRSNS--DFDVNTTFSLLLAAVSTPHNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSER GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALISALAC KRRVALAACNAV+DLSTTSFGR+ LVE SAIE LISAFLQVSASS
Sbjct: 121 ADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSVSIGT+Y ENSAILK GF+GDELSI ILNAAIVL+NTCHFKHLEKIP YY + FL F
Sbjct: 181 AMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           L+ QWIEVRSKMMQIN+ ECSWQ QF  +SNISTNDLAV IFRLSISTD+VSRV  VKEV
Sbjct: 241 LQKQWIEVRSKMMQINKIECSWQEQF-KISNISTNDLAVCIFRLSISTDRVSRVFLVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           KTLLGLSG NFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SIET  SFIS+M
Sbjct: 301 KTLLGLSGLNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+FQ  FEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFQKTFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC+KGPHFLKLNDALKCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC-- 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YC  
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWK 600

Query: 601 WNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
           WNDN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+ LPSD N+   LNQKTI
Sbjct: 601 WNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASSLPSDNNNMLALNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVD KES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQWTMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NAVECYK+LLER+R VRSQY TGMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYTTGMISL 778

BLAST of Lcy03g017680 vs. ExPASy TrEMBL
Match: A0A6J1C3K1 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Momordica charantia OX=3673 GN=LOC111007109 PE=3 SV=1)

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 635/786 (80.79%), Postives = 698/786 (88.80%), Query Frame = 0

Query: 2   EHQKKLCS------HSQTSTSKTKRKRQSNSQT--NFDANTTSSLLLAAVSSPCNPCSAS 61
           + +KKLC        + +S+S++KRKRQSNS +  NF+ ++T SLLLAAVSSP NPCS S
Sbjct: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62

Query: 62  LIPKCLAELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLR 121
           LI KCLAEL  A+ PQSP PLQTLPPPILSLLPLLILS RPGIASRAAEIVGAAS++SLR
Sbjct: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122

Query: 122 MNEVIASDDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAF 181
           +NE IASDD V++ALISALAC  R VALAACNAVLDLSTTSFGR+ LVEVSAIE L+SAF
Sbjct: 123 VNEEIASDDAVLKALISALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182

Query: 182 LQVSASSEMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYY 241
           LQVSASSEMSVS+GT+YNEN A LK GF+GDELSILILNAAIVL+NTCHFKHLEKIP Y 
Sbjct: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242

Query: 242 SRKFLAFLKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSR 301
           S+ FLAFLKNQWIEVR+KM+ IN  +CS Q +FD +S ISTNDLAVSIFRLSISTDQVSR
Sbjct: 243 SQMFLAFLKNQWIEVRNKMVLINSFQCSLQEKFD-ISKISTNDLAVSIFRLSISTDQVSR 302

Query: 302 VLPVKEVKTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETI 361
           ++PVKEVKTL GLSGS FEEFMLNHWEASPLLMQK SRT+NEEADII SF+GSV SI+  
Sbjct: 303 LIPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID-- 362

Query: 362 HSFISTMLGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHY 421
            SF+S MLGRLVSCNPIASDE+DIHNFLEEV+NELGFPLIYQQDIRVLRTD C KREIH+
Sbjct: 363 -SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHF 422

Query: 422 FQNNFEPCCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQP 481
           FQ NFEPC  KGPHFLKLNDALKCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQP
Sbjct: 423 FQENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQP 482

Query: 482 SVGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSE 541
           SVGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +MWLPRLYDAHE   CSE
Sbjct: 483 SVGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSE 542

Query: 542 VESPLAVGRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLH 601
           +E+PLAV RQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGF+H
Sbjct: 543 IENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVH 602

Query: 602 TALYCWNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDL 661
            A+Y WNDN KQYDTS   +S+ELLHFAI LIS+SD NFRKACLVAA  LPSDT++R DL
Sbjct: 603 IAIYSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDL 662

Query: 662 NQKTIFSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWA 721
           NQKTIF++L+DKISRESK LEV++SI+I+VEKDEDPFHRMRWLRLLNVDGKE  KEDKW 
Sbjct: 663 NQKTIFNRLIDKISRESKFLEVISSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWT 722

Query: 722 MPSMGIKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMT 780
           MPS+GI++L   CVN++EKVE TF+EVKSRFC EVRF+NAVECYK+LLERY KVRSQYMT
Sbjct: 723 MPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMT 782

BLAST of Lcy03g017680 vs. ExPASy TrEMBL
Match: A0A1S3BXA4 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103494221 PE=3 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 633/778 (81.36%), Postives = 686/778 (88.17%), Query Frame = 0

Query: 4   QKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLAELH 63
           +KKL SHS  STSKTKRKRQ +   +FD NTT SLLLAAVS+P NP S  L+PKCL  LH
Sbjct: 8   KKKLYSHSHISTSKTKRKRQPH--FDFDVNTTFSLLLAAVSTPHNPYSVFLLPKCLTHLH 67

Query: 64  FALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIASDDG 123
            ALLPQSPHPLQTLP PILSLLPLLILSER GIAS AAEIVGAAS++SLR NEVIA DDG
Sbjct: 68  SALLPQSPHPLQTLPSPILSLLPLLILSERQGIASCAAEIVGAASLLSLRTNEVIALDDG 127

Query: 124 VVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASSEMS 183
           VVRALIS L C KRRVALAACNAVLDLSTTSFGRQRLVE SAI+ LI +FLQVSAS+ MS
Sbjct: 128 VVRALISVLGCSKRRVALAACNAVLDLSTTSFGRQRLVEFSAIQRLIFSFLQVSASTGMS 187

Query: 184 VSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAFLKN 243
           VSIGT+Y ENSAILK G++GDEL I ILNAAIVLINTCHFKHLEKIP Y+ + F+ FLKN
Sbjct: 188 VSIGTVYVENSAILKAGYEGDELLISILNAAIVLINTCHFKHLEKIPSYHLKAFMGFLKN 247

Query: 244 QWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEVKTL 303
            WIEVR KM+QIN  ECS Q QFD MSNISTNDLA SIFRLS+STDQ+SRV PV++VK L
Sbjct: 248 LWIEVRIKMIQINEIECS-QEQFD-MSNISTNDLAASIFRLSMSTDQISRVFPVEKVKIL 307

Query: 304 LGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTMLGR 363
           LGLSGSNFEEFM +HWEASP LMQKSSR INEEAD I SF+GS+ SIE IHSFIS MLGR
Sbjct: 308 LGLSGSNFEEFMASHWEASPCLMQKSSR-INEEADFIGSFVGSITSIEKIHSFISPMLGR 367

Query: 364 LVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEPCCI 423
           LVSC+PIASDE+DIHNFLEE R ELGFPLIYQQDIRVLRTD+CLKREIH+FQ NFEPCCI
Sbjct: 368 LVSCSPIASDELDIHNFLEEARTELGFPLIYQQDIRVLRTDQCLKREIHFFQKNFEPCCI 427

Query: 424 KGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTP 483
           KGPHFLKL+DALKCEEAF EGYTIALRGMEFR+EKIAAI+NTLASLFGQPSVGANMYLTP
Sbjct: 428 KGPHFLKLHDALKCEEAFKEGYTIALRGMEFRHEKIAAISNTLASLFGQPSVGANMYLTP 487

Query: 484 PGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAVGRQ 543
           PGSQGL RHYDDHCVFVCQLAGSKQWTVFSPP++ LPRLYD HEFP CSEVES LAVGRQ
Sbjct: 488 PGSQGLTRHYDDHCVFVCQLAGSKQWTVFSPPRIRLPRLYDFHEFPSCSEVESALAVGRQ 547

Query: 544 FLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYCWN--D 603
           F LREGDVLYIPRGFLHEART + GPDGSSLHLTFGIEVEPPFEWEGF+HTA+Y WN   
Sbjct: 548 FFLREGDVLYIPRGFLHEARTVSGGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYSWNWSH 607

Query: 604 NSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTIFSK 663
           N KQ DT F T+SV+LLHFAIWLISVSD NFRKACLVAA  LPS+TN R  L QKT FS+
Sbjct: 608 NPKQCDTLFGTMSVQLLHFAIWLISVSDHNFRKACLVAACSLPSETNDRLGLIQKTTFSR 667

Query: 664 LLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMGIKN 723
           LLDKIS ESK  EVLA++KISVEKDEDPFHRMRWLRLLNVDGKES K+DKW MPS+GI++
Sbjct: 668 LLDKISSESKFSEVLAALKISVEKDEDPFHRMRWLRLLNVDGKESIKDDKWTMPSIGIRD 727

Query: 724 LLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISFH 780
           LL TCVNHRE++ TTFVEVKSRFCREVRF+NAVECY++LL+R+RKVRSQY  GMIS H
Sbjct: 728 LLSTCVNHREQIGTTFVEVKSRFCREVRFKNAVECYQKLLKRFRKVRSQYTNGMISLH 780

BLAST of Lcy03g017680 vs. ExPASy TrEMBL
Match: A0A0A0LWZ8 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G427540 PE=3 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 635/783 (81.10%), Postives = 690/783 (88.12%), Query Frame = 0

Query: 1   MEHQKKLC--SHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKC 60
           ++ +K+ C  SHS TS+SKTKRKRQ +   +FD NTT SLLLAAVS+P NP S SL+PKC
Sbjct: 5   LKKKKEPCSYSHSHTSSSKTKRKRQPH--FDFDVNTTFSLLLAAVSTPHNPYSVSLLPKC 64

Query: 61  LAELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVI 120
           L  LH ALLPQSPHP QTLP  ILSLLPLLILSER GIAS AAEIVGAAS++SLRMNEVI
Sbjct: 65  LTHLHSALLPQSPHPFQTLPSSILSLLPLLILSERQGIASCAAEIVGAASLLSLRMNEVI 124

Query: 121 ASDDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSA 180
           ASDDG+VRALIS L C KRRVALAACNAVLDLSTTSFGRQRLVE SAI+ LI   + VSA
Sbjct: 125 ASDDGLVRALISVLGCSKRRVALAACNAVLDLSTTSFGRQRLVEFSAIQRLI--VILVSA 184

Query: 181 SSEMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFL 240
           S+ MSVSIGT+Y ENSAILK GF+GDEL I ILNAAIVLIN CHF+HLEKIP Y+ + F+
Sbjct: 185 STGMSVSIGTVYIENSAILKAGFEGDELLISILNAAIVLINICHFEHLEKIPIYHLKAFM 244

Query: 241 AFLKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVK 300
            FLKNQWIEVRSKMMQIN  ECS Q QFD MSNISTNDLA  IFRLS+STDQVSRV PVK
Sbjct: 245 GFLKNQWIEVRSKMMQINEIECS-QEQFD-MSNISTNDLAACIFRLSMSTDQVSRVFPVK 304

Query: 301 EVKTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFIS 360
           EVKTLLGLSGSNFEEFM++HWE SP LMQKSSR INEEADII SF+GS+ SIE  HSFIS
Sbjct: 305 EVKTLLGLSGSNFEEFMVSHWETSPCLMQKSSR-INEEADIIGSFVGSITSIEKNHSFIS 364

Query: 361 TMLGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNF 420
            MLGRLVSC+PIASDE+DIHNFLEE R ELGFPLIYQQDIRVLRTDECLKREIH+FQ NF
Sbjct: 365 PMLGRLVSCSPIASDELDIHNFLEEARAELGFPLIYQQDIRVLRTDECLKREIHFFQKNF 424

Query: 421 EPCCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGAN 480
           EPCCI+GPHFLKL+DALKCEEAF EGYTIALRGMEFR+EKIAAI+NTLASLFGQPSVGAN
Sbjct: 425 EPCCIEGPHFLKLHDALKCEEAFKEGYTIALRGMEFRHEKIAAISNTLASLFGQPSVGAN 484

Query: 481 MYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPL 540
           MYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPP+ +LPRLYD+HEFP C +VESPL
Sbjct: 485 MYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPRKYLPRLYDSHEFPSCLDVESPL 544

Query: 541 AVGRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC 600
           AVGR+F LREGDVLYIPRGFLHEARTE  GPDGSSLHLTFGIEVEPPFEWEGF+HTA+Y 
Sbjct: 545 AVGRKFFLREGDVLYIPRGFLHEARTEIGGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYS 604

Query: 601 WN--DNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQK 660
           WN   N KQ DT F T+S+ELLHFAIWLISVSD NFRKACLVAA  LPS+TN R  LN K
Sbjct: 605 WNWSHNPKQCDTLFGTMSLELLHFAIWLISVSDHNFRKACLVAACSLPSETNDRLGLNLK 664

Query: 661 TIFSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPS 720
           T+FS+LLDKISRESK  EVLA+IKISVEKDEDPFHRMRWLRLLNVDGKES K+DKW MPS
Sbjct: 665 TVFSRLLDKISRESKFSEVLAAIKISVEKDEDPFHRMRWLRLLNVDGKESIKDDKWTMPS 724

Query: 721 MGIKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMI 780
           +GI++L  TCVNHRE+V TTFVEVKSRFC EVRF+NAVECYK+LL+R+RKVRSQY  GMI
Sbjct: 725 IGIRDLFSTCVNHREQVATTFVEVKSRFCSEVRFENAVECYKKLLKRFRKVRSQYTKGMI 780

BLAST of Lcy03g017680 vs. NCBI nr
Match: XP_023515380.1 (uncharacterized protein LOC111779552 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 673/781 (86.17%), Postives = 713/781 (91.29%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS TSTSKTKRKR+SNS  +FD NTT SLLLAAVS+P NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSCTSTSKTKRKRRSNS--DFDVNTTFSLLLAAVSTPRNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSER GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALISALAC KRRVALAACNAV+DL TTSFGR+ LVE SAIE LISAFLQVSASS
Sbjct: 121 ADGVVRALISALACSKRRVALAACNAVMDLCTTSFGRESLVEFSAIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSVSIGT+Y ENSAILK GF+GDELS+ ILNAAIVL+NTCHFKHLEKIP YY + FL F
Sbjct: 181 AMSVSIGTVYIENSAILKAGFEGDELSVSILNAAIVLLNTCHFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           LK QWIEVRSKMMQIN+ ECSWQ QF+ +SNISTNDLAV IFRLSISTDQVSRV PVKEV
Sbjct: 241 LKKQWIEVRSKMMQINKIECSWQEQFE-ISNISTNDLAVCIFRLSISTDQVSRVFPVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           KTLLGLSGSNFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SIET  SFIS+M
Sbjct: 301 KTLLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+FQ  FEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFQKTFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC+KGPHFLKLNDALKCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYCWN 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YCWN
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWN 600

Query: 601 --DNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
             DN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+FLPSD N+  D NQKTI
Sbjct: 601 WKDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNMLDQNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVDGKES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQWTMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NA ECYK+LLER+R VRSQY  GMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAAECYKKLLERFRNVRSQYTIGMISL 778

BLAST of Lcy03g017680 vs. NCBI nr
Match: XP_022987328.1 (uncharacterized protein LOC111484915 [Cucurbita maxima])

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 670/781 (85.79%), Postives = 714/781 (91.42%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS+TSTSKTKRKR+SNS  +FD NTT SLLLAAVS+P NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSRTSTSKTKRKRRSNS--DFDVNTTFSLLLAAVSTPRNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSER GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALIS LAC KRRVALAACNAV+DLSTTSFGR+ LVE SAIE LISAFLQVSASS
Sbjct: 121 ADGVVRALISTLACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSV IGT+Y ENSAILK GF+GDELSI IL+AAIVL+NTC+FKHLEKIP YY + FL F
Sbjct: 181 AMSVFIGTVYIENSAILKAGFEGDELSISILSAAIVLLNTCYFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           LK QWIEV SKMMQIN++ECSWQ QF+ +SNISTNDLAV IFRLSISTDQVSR+ PVKEV
Sbjct: 241 LKKQWIEVCSKMMQINKTECSWQEQFE-ISNISTNDLAVCIFRLSISTDQVSRIFPVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           K LLGLSGSNFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SI+T  SFIS+M
Sbjct: 301 KALLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIKTNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+FQ NFEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFQKNFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC KGPHFLKLNDA KCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSP KM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPTKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC-- 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YC  
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWN 600

Query: 601 WNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
           WNDN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+FLPSD N+R DLNQKTI
Sbjct: 601 WNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNRPDLNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVDGKES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQWIMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NAVECYK+LLER+R VRSQY TGMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYTTGMISL 778

BLAST of Lcy03g017680 vs. NCBI nr
Match: XP_022921349.1 (uncharacterized protein LOC111429646 [Cucurbita moschata])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 671/781 (85.92%), Postives = 711/781 (91.04%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS TSTSKTKRKR+SNS  +FD NTT SLLLAAVS+P NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSCTSTSKTKRKRRSNS--DFDVNTTFSLLLAAVSTPHNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSER GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALISALAC KRRVALAACNAV+DLSTTSFGR+ LVE SAIE LISAFLQVSASS
Sbjct: 121 ADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSVSIGT+Y ENSAILK GF+GDELSI ILNAAIVL+NTCHFKHLEKIP YY + FL F
Sbjct: 181 AMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           L+ QWIEVRSKMMQIN+ ECSWQ QF  +SNISTNDLAV IFRLSISTD+VSRV  VKEV
Sbjct: 241 LQKQWIEVRSKMMQINKIECSWQEQF-KISNISTNDLAVCIFRLSISTDRVSRVFLVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           KTLLGLSG NFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SIET  SFIS+M
Sbjct: 301 KTLLGLSGLNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+FQ  FEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFQKTFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC+KGPHFLKLNDALKCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC-- 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YC  
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWK 600

Query: 601 WNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
           WNDN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+ LPSD N+   LNQKTI
Sbjct: 601 WNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASSLPSDNNNMLALNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVD KES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQWTMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NAVECYK+LLER+R VRSQY TGMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYTTGMISL 778

BLAST of Lcy03g017680 vs. NCBI nr
Match: KAG7023352.1 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1301.6 bits (3367), Expect = 0.0e+00
Identity = 668/781 (85.53%), Postives = 708/781 (90.65%), Query Frame = 0

Query: 1   MEHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLA 60
           ME Q+KLCSHS TSTSKTKRKR+SNS  +FD NTT  LLLAAVS+  NP +ASLIPKCLA
Sbjct: 1   MEQQQKLCSHSCTSTSKTKRKRRSNS--DFDVNTTFCLLLAAVSTRRNPYAASLIPKCLA 60

Query: 61  ELHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIAS 120
           ELHFALLP SPHPLQTLP  ILSLLPLLILSE  GIASRAAEIVGAAS++SLRMNEVIAS
Sbjct: 61  ELHFALLPHSPHPLQTLPSSILSLLPLLILSELQGIASRAAEIVGAASLLSLRMNEVIAS 120

Query: 121 DDGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASS 180
            DGVVRALISALAC KRRVALAACNAV+DLSTTSFGR+ LVE S IE LISAFLQVSASS
Sbjct: 121 ADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSTIERLISAFLQVSASS 180

Query: 181 EMSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAF 240
            MSVSIGT+Y ENSAILK GF+GDELSI ILNAAIVL+NTCHFKHLEKIP YY + FL F
Sbjct: 181 AMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYYLKTFLTF 240

Query: 241 LKNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEV 300
           L+ QWIEVRSKMMQIN+ ECSWQ QF  +SNISTNDLAV IFRLSISTD+VSRV PVKEV
Sbjct: 241 LQKQWIEVRSKMMQINKIECSWQEQF-KISNISTNDLAVCIFRLSISTDRVSRVFPVKEV 300

Query: 301 KTLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTM 360
           K LLGLSGSNFEEFMLNHWE+SP LMQKSS TINEEADII SF+GS+ SIET  SFIS+M
Sbjct: 301 KMLLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNLSFISSM 360

Query: 361 LGRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEP 420
           LGRLVSCNPIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDECLKREIH+F+ NFEP
Sbjct: 361 LGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFFRKNFEP 420

Query: 421 CCIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480
           CC KGPHFLKLNDA KCEEAF EGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY
Sbjct: 421 CCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMY 480

Query: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAV 540
           LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKM LPRLYDA+EFPRCSEVESPLAV
Sbjct: 481 LTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEVESPLAV 540

Query: 541 GRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYC-- 600
           GRQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGF+HTA+YC  
Sbjct: 541 GRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHTAVYCWN 600

Query: 601 WNDNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTI 660
           WNDN KQYDT F T+SVELLH AIWLISVSD NFRKACLV A+FLPSD N+   LNQKTI
Sbjct: 601 WNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNMLALNQKTI 660

Query: 661 FSKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMG 720
           FS+L+DKISRES  LEVLA IKISVEKDEDPFHRMRWLRLLNVD KES KED+W MPS+G
Sbjct: 661 FSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQWTMPSIG 720

Query: 721 IKNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISF 780
           I++LL TC  HREKVETTFVEVKSRFCREVRF+NAVECYK+LLER+R VRSQY TGMIS 
Sbjct: 721 IRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYTTGMISL 778

BLAST of Lcy03g017680 vs. NCBI nr
Match: XP_038878778.1 (uncharacterized protein LOC120070934 isoform X2 [Benincasa hispida])

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 643/780 (82.44%), Postives = 698/780 (89.49%), Query Frame = 0

Query: 2   EHQKKLCSHSQTSTSKTKRKRQSNSQTNFDANTTSSLLLAAVSSPCNPCSASLIPKCLAE 61
           + +K+LCSHS+TS+SK KRKRQ +S   FD NTT SLLLAAVS+P NP SASLIPKCL  
Sbjct: 5   QQKKRLCSHSRTSSSKGKRKRQPHSL--FDLNTTFSLLLAAVSNPHNPYSASLIPKCLTH 64

Query: 62  LHFALLPQSPHPLQTLPPPILSLLPLLILSERPGIASRAAEIVGAASIMSLRMNEVIASD 121
           LH ALLPQSPHPL TLPPPILSLLPLLILS+R  IASRAAEIVG+AS++SLRMNEVIA D
Sbjct: 65  LHSALLPQSPHPLHTLPPPILSLLPLLILSQRQRIASRAAEIVGSASLLSLRMNEVIALD 124

Query: 122 DGVVRALISALACLKRRVALAACNAVLDLSTTSFGRQRLVEVSAIECLISAFLQVSASSE 181
           DGVVRALISALAC KR VALAACNA+LDLSTTSFGRQ LV  SAI+ LISAFLQVSASSE
Sbjct: 125 DGVVRALISALACSKRMVALAACNALLDLSTTSFGRQSLVHFSAIQRLISAFLQVSASSE 184

Query: 182 MSVSIGTIYNENSAILKVGFKGDELSILILNAAIVLINTCHFKHLEKIPCYYSRKFLAFL 241
           MSVS+GT+YNENS  LK  F+GDELS+ ILNAAIVLINTCHF HLEKIP Y+ + FLAFL
Sbjct: 185 MSVSVGTVYNENSVSLKARFEGDELSVSILNAAIVLINTCHFIHLEKIPGYHLKAFLAFL 244

Query: 242 KNQWIEVRSKMMQINRSECSWQGQFDMMSNISTNDLAVSIFRLSISTDQVSRVLPVKEVK 301
           +NQWIEVRSKMMQIN+ +CS Q QFD MSNISTNDLAVSIFRLS+STD+VS V PVKEVK
Sbjct: 245 RNQWIEVRSKMMQINKIKCS-QEQFD-MSNISTNDLAVSIFRLSVSTDRVSGVFPVKEVK 304

Query: 302 TLLGLSGSNFEEFMLNHWEASPLLMQKSSRTINEEADIISSFLGSVASIETIHSFISTML 361
           TLLGLSGSNFEEFMLNHWEASP LM+KSSRTINEEADII SF+GS+ SIE IHSF+S ML
Sbjct: 305 TLLGLSGSNFEEFMLNHWEASPWLMRKSSRTINEEADIIGSFMGSMTSIEKIHSFVSPML 364

Query: 362 GRLVSCNPIASDEIDIHNFLEEVRNELGFPLIYQQDIRVLRTDECLKREIHYFQNNFEPC 421
           GRLVSC+PIASDE+DIHNFLEE RNELGFPLIYQQDIRVLRTDE LK+EIH+F  NFEPC
Sbjct: 365 GRLVSCSPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDERLKKEIHFFHKNFEPC 424

Query: 422 CIKGPHFLKLNDALKCEEAFNEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYL 481
           C KGPHFLKLNDALKCEEAF EGYTIALRGMEFR EKIAAIANT+A LFGQPSVGANMYL
Sbjct: 425 CTKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRCEKIAAIANTVACLFGQPSVGANMYL 484

Query: 482 TPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMWLPRLYDAHEFPRCSEVESPLAVG 541
           TPP SQGLARHYDDHCVFVCQLAGSKQWTVFSPP+  LPRLYD+HEFP CSEVESP  VG
Sbjct: 485 TPPSSQGLARHYDDHCVFVCQLAGSKQWTVFSPPRTCLPRLYDSHEFPSCSEVESPFTVG 544

Query: 542 RQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFLHTALYCWN- 601
           RQFLLREGDVLYIPRGFLHEART NDGPDGSSLHLTFGIE+EPPFEWEGF+HTA+YCWN 
Sbjct: 545 RQFLLREGDVLYIPRGFLHEARTVNDGPDGSSLHLTFGIEIEPPFEWEGFVHTAVYCWNW 604

Query: 602 -DNSKQYDTSFWTISVELLHFAIWLISVSDQNFRKACLVAANFLPSDTNSRFDLNQKTIF 661
             N K+YDT F  +S+ELLHFAIWLISVSD NFRKACLVAA+ L SDTN+R DLNQK  F
Sbjct: 605 SHNPKEYDTLFAPMSLELLHFAIWLISVSDHNFRKACLVAASSLASDTNNRLDLNQKATF 664

Query: 662 SKLLDKISRESKILEVLASIKISVEKDEDPFHRMRWLRLLNVDGKESFKEDKWAMPSMGI 721
           S+L+DKISRESK LEV+A IKIS+EKDEDPFHRMRW+RLLNVDGKES KE KW MPS+GI
Sbjct: 665 SRLIDKISRESKFLEVVAGIKISIEKDEDPFHRMRWIRLLNVDGKESVKEGKWTMPSIGI 724

Query: 722 KNLLLTCVNHREKVETTFVEVKSRFCREVRFQNAVECYKQLLERYRKVRSQYMTGMISFH 780
           K+LL TCVNHREK+ET FVEVKSRFC EVRF+NAVECYK+LL+R+RKVRSQY  GMIS H
Sbjct: 725 KDLLSTCVNHREKMETIFVEVKSRFCSEVRFENAVECYKKLLKRFRKVRSQYTKGMISLH 780