Lcy03g017480 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g017480 vs. ExPASy Swiss-Prot
Match: Q9K9K5 (Arginine decarboxylase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=speA PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.3e-88
Identity = 182/482 (37.76%), Postives = 267/482 (55.39%), Query Frame = 0

Query: 74  PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGP 133
           PL   + A A+ N   FH PGHK+G     +    IG      D   +  LD+L  P G 
Sbjct: 8   PLFSGVVAHAKHNPVQFHIPGHKKGAGMDPAFRSFIGDNALSIDLINIGPLDDLHHPHGI 67

Query: 134 ILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTG 193
           I EAQ+ AA+ FGA  T+F V GT+  I   IM+   PGE II+PRN H S +SA+VF+G
Sbjct: 68  IKEAQELAAEAFGADHTFFSVQGTSGAIMTMIMSVVGPGEKIIVPRNVHKSIMSAIVFSG 127

Query: 194 ANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEIS 253
           A P++I PE D    I+ G+T   V KA++           + V +PTY GI +NL +I 
Sbjct: 128 ATPVFIHPEIDPLLGISHGITIEAVEKALDAH----PDAKGLLVINPTYFGIAANLKKIV 187

Query: 254 QICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGN 313
           ++CHS+ +P++VDEAHG H  F   LP SA+Q GAD+   S HK+  SLTQSS+L++   
Sbjct: 188 ELCHSRDVPVLVDEAHGVHIHFHEALPLSAMQAGADMAATSVHKLGGSLTQSSILNVREG 247

Query: 314 IIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKIS 373
           ++  +RV   +  L +TS SYLLLASLDAAR  L+ N   +    I LA+QA+ +IN I 
Sbjct: 248 LVSAKRVQTIISMLTTTSTSYLLLASLDAARKHLATNGRDLIGYTIQLADQARDQINAID 307

Query: 374 DISVLEFPIFSNFPA--IDPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSITIV 433
            +  +   I         DP +L I  + LG++GY+A   L +++ +  EL    +I  +
Sbjct: 308 GLYCVGKEILGTIATYDYDPTKLIISVKNLGITGYDAEVWLREHYQIEVELSDLYNILCI 367

Query: 434 INLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMNPREAFFAKKGK 493
           ++ G  E ++  LV  L +++      GI +RS ++++ P     + M+PR+AF+A+   
Sbjct: 368 VSFGDTEREMDLLVKALSELADLHK-HGICERSPVSVYVPNIP-TLAMSPRDAFYAETEV 427

Query: 494 QSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSITGASDPQLSSL 553
                SVG    E    +PPGIP+ IPGE+ITE    ++    R G+ + G  D    +L
Sbjct: 428 VPFEDSVGRTIAEFIMVYPPGIPILIPGEIITESNLAYIRENNRAGLPVQGPEDDTFRTL 483

BLAST of Lcy03g017480 vs. ExPASy Swiss-Prot
Match: P21885 (Arginine decarboxylase OS=Bacillus subtilis (strain 168) OX=224308 GN=speA PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 1.0e-83
Identity = 173/488 (35.45%), Postives = 265/488 (54.30%), Query Frame = 0

Query: 74  PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGP 133
           PL   LK  A +    FH PGHK+G        Q IG      D   +  LD+L  P+G 
Sbjct: 7   PLYTGLKKHASRQPVQFHIPGHKKGAGMDPEFRQFIGENALSIDLINIEPLDDLHAPKGI 66

Query: 134 ILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTG 193
           I +AQ  AA+ FGA  T+F V GT+  I   +MA C PG+ II+PRN H S ++A+VF+G
Sbjct: 67  IKQAQDLAAEAFGADHTFFSVQGTSGAIMTMVMAVCGPGDKIIIPRNVHKSIMTAIVFSG 126

Query: 194 ANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEIS 253
           A PI+I PE D+   I+ G+T     +A+     E      + V +PTY G+ ++L  I 
Sbjct: 127 AVPIFIHPEIDNELGISHGITLESAKRAL----TEHPDAKGLLVINPTYFGVAADLKSIV 186

Query: 254 QICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGN 313
           ++ HS  +P++VDEAHG H  F  +LP SA+Q GAD+   S HK+  SLTQSS+L++   
Sbjct: 187 ELAHSFDVPVLVDEAHGVHIHFHDELPLSAMQAGADIAATSVHKLGGSLTQSSILNMREG 246

Query: 314 IIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKIS 373
           ++ ++RV   L  L +TS SYLLLASLD AR +L+    ++  + + LANQ + ++N+I 
Sbjct: 247 LVSKDRVQSILSMLTTTSTSYLLLASLDVARKRLATEGRQLAEETLKLANQTRDRLNQIE 306

Query: 374 DISVLEFPIFSNFPA--IDPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSITIV 433
            I  +   I  +  A   DP +L I  + LGL+G++  + L ++  +  EL    +I  +
Sbjct: 307 GIYCVGSEILGSKAAYSYDPTKLIISVKSLGLTGHDVEKWLRESFNIEVELSDLYNILCI 366

Query: 434 INLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDI------KIGMNPREAF 493
              G  ++D  RLV  L +++        +Q S+ ++    T++       + M PR+AF
Sbjct: 367 FTPGDSQNDADRLVEALTEIA--------QQMSEQDVTHQQTEVLLPEIPLLAMTPRDAF 426

Query: 494 FAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSITGASD 553
           +A      +  + G +  E    +PPGIP+ IPGE+ITEE   ++      G+ + G  D
Sbjct: 427 YANTEVIPLKEASGRIIAEFVMVYPPGIPIFIPGEIITEENISYIFKNLDAGLPVQGPED 482

BLAST of Lcy03g017480 vs. ExPASy Swiss-Prot
Match: Q81MS2 (Arginine decarboxylase OS=Bacillus anthracis OX=1392 GN=speA PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.7e-81
Identity = 165/486 (33.95%), Postives = 264/486 (54.32%), Query Frame = 0

Query: 74  PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGP 133
           PL  AL   +++N   FH PGHK+GQ       + IG      D   +  LD+L  P+G 
Sbjct: 10  PLFTALVEHSKRNPIQFHIPGHKKGQGMDPEFREFIGHNALAIDLINIAPLDDLHHPKGM 69

Query: 134 ILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTG 193
           I EAQ  AA  FGA  T+F + GT+  I   +M+ C PG+ I++PRN H S +SA++F+G
Sbjct: 70  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 129

Query: 194 ANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEIS 253
           A PI++ PE D    I+ G+T   V KA+E    E      + V +PTY G  ++L +I 
Sbjct: 130 AKPIFMHPEIDPKLGISHGITIQSVKKALE----EHSDAKGLLVINPTYFGFAADLEQIV 189

Query: 254 QICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGN 313
           Q+ HS  IP++VDEAHG H  F  +LP SA+Q GAD+   S HK+  SLTQSS+L++   
Sbjct: 190 QLAHSYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEG 249

Query: 314 IIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKIS 373
           +++ + V   +  L +TS SY+LLASLD AR +L+     +  + I LA Q +  IN I 
Sbjct: 250 LVNVKHVQSIISMLTTTSTSYILLASLDVARKRLATEGKALIEQTIQLAEQVRNAINDIE 309

Query: 374 DI-----SVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSI 433
            +      +L      N+   DP ++ +  + LG++G++A   L + + +  EL    +I
Sbjct: 310 HLYCPGKEMLGTDATFNY---DPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNI 369

Query: 434 TIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKI-GMNPREAFFA 493
             ++  G  E +   L++ L+D+S+   I   +    + I     +I +  ++PR+AF++
Sbjct: 370 LCLVTFGDTESETNTLIAALQDLSA---IFKNKADKGVRIQVEIPEIPVLALSPRDAFYS 429

Query: 494 KKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSITGASDPQ 553
           +        + G +  +    +PPGIP+  PGE+IT++  +++      G+ + G  D  
Sbjct: 430 ETEVIPFENAAGRIIADFVMVYPPGIPIFTPGEIITQDNLEYIRKNLEAGLPVQGPEDMT 485

BLAST of Lcy03g017480 vs. ExPASy Swiss-Prot
Match: Q819L4 (Arginine decarboxylase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=speA PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.4e-75
Identity = 155/451 (34.37%), Postives = 248/451 (54.99%), Query Frame = 0

Query: 74  PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGP 133
           PL  AL   +++N   FH PGHK+GQ    +  + IG      D   +  LD+L  P+G 
Sbjct: 7   PLFTALVEHSKRNPIQFHIPGHKKGQGMDPTFREFIGHNALAIDLINIAPLDDLHHPKGM 66

Query: 134 ILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTG 193
           I EAQ  AA  FGA  T+F + GT+  I   +M+ C PG+ I++PRN H S +SA++F+G
Sbjct: 67  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 126

Query: 194 ANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEIS 253
           A PI++ PE D    I+ G+T   V KA+E    E      + V +PTY G  ++L +I 
Sbjct: 127 AKPIFMHPEIDPKLGISHGITIQSVKKALE----EHSDAKGLLVINPTYFGFAADLEQIV 186

Query: 254 QICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGN 313
           Q+ HS  IP++VDEAHG H  F  +LP SA+Q GAD+   S HK+  SLTQSS+L++   
Sbjct: 187 QLAHSYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEG 246

Query: 314 IIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKIS 373
           +++ + V   +  L +TS SY+LLASLD AR +L+     +  + I LA   +  IN I 
Sbjct: 247 LVNVKHVQSIISMLTTTSTSYILLASLDVARKRLATEGTALIEQTIQLAEHVRDAINSIE 306

Query: 374 DI-----SVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSI 433
            +      +L      N+   DP ++ +  + LG++G++A   L + + +  EL    +I
Sbjct: 307 HLYCPGKEMLGTDATFNY---DPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNI 366

Query: 434 TIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMNPREAFFAK 493
             +I LG  E D   L++ L+D+++       ++  ++ +  P   + + ++PR+AF+++
Sbjct: 367 LCLITLGDTESDTNTLIAALQDLAATFRNRA-DKGVQVQVEIPEIPV-LALSPRDAFYSE 426

Query: 494 KGKQSITRSVGEVCGELTCPFPPGIPVTIPG 520
                   + G +  +    +PPGIP+  PG
Sbjct: 427 TEVIPFENAAGRIIADFVMVYPPGIPIFTPG 448

BLAST of Lcy03g017480 vs. ExPASy Swiss-Prot
Match: P37536 (Uncharacterized protein YaaO OS=Bacillus subtilis (strain 168) OX=224308 GN=yaaO PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.5e-61
Identity = 161/461 (34.92%), Postives = 244/461 (52.93%), Query Frame = 0

Query: 72  NPPLIRALKASAEQNTADFHFPGHKRGQA----APSSITQLIGLKPFMHDFSQVPELDNL 131
           N PL +AL   A +N+  FH PGH  G      A S    L+ +     D +++  LD+L
Sbjct: 2   NTPLYKALIQHARRNSHSFHVPGHHNGDVFFDDAKSIFDPLLTI-----DVTELAGLDDL 61

Query: 132 FRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAIS 191
             P G I EAQ+ A++L+G++E++FLV GTT G  A I++ C PG+ I++ RN H S   
Sbjct: 62  HHPSGVIKEAQELASQLYGSAESFFLVNGTTVGNLAMILSVCEPGDTILVQRNCHKSVFH 121

Query: 192 ALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICS 251
           A+  +GA P+Y+ P+ DS   +   V    + +A+E           + +T+PTY+G  +
Sbjct: 122 AVDLSGAEPVYLAPDVDSAMHVPTHVPLGTIKEALE----AYPDAKGLVLTNPTYYGHSA 181

Query: 252 NLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSM 311
           +L+EI    H  GIP++VDEAHGAHF      P SAL+ GAD+VVQS HK L ++T  S 
Sbjct: 182 DLTEIITEAHHYGIPVLVDEAHGAHFILGEPFPVSALKMGADIVVQSAHKTLPAMTMGSY 241

Query: 312 LHLSGNI-IDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAK 371
           LHL+ +  I+R+RV   L  LQS+SPSY ++ASLD ARA +    ++   K  D+  + +
Sbjct: 242 LHLNSSCRINRDRVAEYLNRLQSSSPSYPIMASLDIARAYVQHIIEE--QKLSDILQRIE 301

Query: 372 RKINKISDISVLEFPIFSNFPAI--DPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIG 431
                   ++  E    +N P I  DPL+LTI   K G SGY    IL + + +  EL  
Sbjct: 302 TLKQTFDSLTNAEAVNPAN-PLIITDPLKLTIR-SKRGHSGYTLQSILERAN-IFTELAD 361

Query: 432 TQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMNPREA 491
              + +V+ LG        ++  +++     P       ++  +  P T +        +
Sbjct: 362 ENQVLLVLPLGGKRRINAEIIRSIDEEIEKTPPDQTFVSAEWGV-QPVTVLPYPKKVLHS 421

Query: 492 FFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEE 526
           F  KK   S   + G +  E   P+PPGIP+ + GE IT+E
Sbjct: 422 F--KKEYVSFEEAAGRLNAEDIIPYPPGIPMIMAGERITKE 445

BLAST of Lcy03g017480 vs. ExPASy TrEMBL
Match: A0A6J1BY72 (uncharacterized protein LOC111006813 OS=Momordica charantia OX=3673 GN=LOC111006813 PE=3 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 8.2e-249
Identity = 433/527 (82.16%), Postives = 476/527 (90.32%), Query Frame = 0

Query: 32  YFKVLQETSIVET--NNNQDRSSKEQNDKPVIRDSPRSQEGSNPPLIRALKASAEQNTAD 91
           + ++     ++ET  NNNQDRSSK+QN     RDSPR QE S+PPLIRALK SAEQN AD
Sbjct: 23  FVRLYSSIFLLETKPNNNQDRSSKKQNQN--ARDSPRFQESSDPPLIRALKVSAEQNAAD 82

Query: 92  FHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGPILEAQQQAAKLFGASE 151
           FHFPGH RG+AAPSS+TQLIGLKPFMHD  Q PELDNLFRPEGPILEA Q+AA+LFGASE
Sbjct: 83  FHFPGHNRGRAAPSSLTQLIGLKPFMHDLPQFPELDNLFRPEGPILEALQEAAQLFGASE 142

Query: 152 TWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTGANPIYIIPEYDSNWDI 211
           TWFLVGGTTSGIQAAIMATCSPGE+IILPRNSH SAISALVF+GA P+YI+PEYDSNWDI
Sbjct: 143 TWFLVGGTTSGIQAAIMATCSPGEHIILPRNSHVSAISALVFSGAKPVYIMPEYDSNWDI 202

Query: 212 AGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEISQICHSKGIPLIVDEAH 271
           AGGVTPSQV +AI+ SEME +KVSAVFVTSPTYHGICSNLSEIS+ICHS GIPLIVDEAH
Sbjct: 203 AGGVTPSQVSRAIQISEMEGKKVSAVFVTSPTYHGICSNLSEISKICHSNGIPLIVDEAH 262

Query: 272 GAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGNIIDRERVCRCLQTLQS 331
           GAHFGF PQLPHSALQQGADLVVQSTHKVLCSLTQSSMLH+SGN +DRERVCR LQ+LQS
Sbjct: 263 GAHFGFHPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHMSGNTVDRERVCRSLQSLQS 322

Query: 332 TSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKISDISVLEFPIFSNFPAI 391
           TSPSYLLLASLDAARAQ+SDNPD+IFNKAIDLANQAK+KI KIS IS+ E PIF NFPAI
Sbjct: 323 TSPSYLLLASLDAARAQISDNPDEIFNKAIDLANQAKQKITKISGISIPELPIFPNFPAI 382

Query: 392 DPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSITIVINLGICEHDIQRLVSGLE 451
           DPLRLTIGFQ+LGLSGY+A+EILHKNHG+ CELIGTQS+T+VINLGICE DIQRLVSGLE
Sbjct: 383 DPLRLTIGFQQLGLSGYKADEILHKNHGLICELIGTQSVTLVINLGICERDIQRLVSGLE 442

Query: 452 DVSSFGPILGIEQRSKLNIFAPFTDIKIGMNPREAFFAKKGKQSITRSVGEVCGELTCPF 511
           DVSS+G IL IE RSK+ + APFTDI+I MNPR+AFFA+K +++I  SVGEVCGEL CP+
Sbjct: 443 DVSSYGSILRIEGRSKVIVSAPFTDIRIKMNPRDAFFARKRRENIRESVGEVCGELICPY 502

Query: 512 PPGIPVTIPGEVITEEVRDFLLHLKRKGVSITGASDPQLSSLVVCNV 557
           PPGIPVTIPGEVITEEVRDFLLHLK KG  ITGASDPQLSS+VVCNV
Sbjct: 503 PPGIPVTIPGEVITEEVRDFLLHLKNKGACITGASDPQLSSIVVCNV 547

BLAST of Lcy03g017480 vs. ExPASy TrEMBL
Match: A0A6J1C001 (uncharacterized protein LOC111007031 OS=Momordica charantia OX=3673 GN=LOC111007031 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.4e-245
Identity = 442/557 (79.35%), Postives = 480/557 (86.18%), Query Frame = 0

Query: 1   MASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQND-KP 60
           MAS  LS S +  +HFR A+N     R RK  F V QETSIVE  NNQDRSSK+Q+  K 
Sbjct: 7   MASSPLSSSTSLNHHFRSAHN-----RGRKSCFSVRQETSIVEPKNNQDRSSKKQSTAKI 66

Query: 61  VIRDSPRSQEGSNPPLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFS 120
            I +SP S+E S PPL+ ALK SAEQN A FHFPGH RG+AAPSS TQLIGLKPFMHD  
Sbjct: 67  AISESPISKE-SCPPLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLP 126

Query: 121 QVPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPR 180
           ++PELDNLF PEGPILEAQQQAAKLFGA ETWFLVGGTT GIQ AIMATCSPGE+II+PR
Sbjct: 127 ELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPR 186

Query: 181 NSHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTS 240
           NSH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV KAI+DSEME  KVSAVFVTS
Sbjct: 187 NSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPSQVDKAIKDSEMEGHKVSAVFVTS 246

Query: 241 PTYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVL 300
           PTYHGICSNLSEISQICHS GIPLIVDEAHGAHFGFQPQ+PHSALQQG DLV QSTHKVL
Sbjct: 247 PTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVL 306

Query: 301 CSLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 360
            SLTQSSMLH+SGNI+DRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI
Sbjct: 307 SSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 366

Query: 361 DLANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVT 420
           DLANQAK KINKIS IS+LE PIFSN PAIDPLRLTIGFQ+LGLSGYEA+EILHKNH + 
Sbjct: 367 DLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTIGFQQLGLSGYEADEILHKNHDIV 426

Query: 421 CELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGM 480
           CEL+GTQSIT VINLG CE DI+RLVSG+EDVSS   IL IE RSKL+  APF DIKI +
Sbjct: 427 CELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIVL 486

Query: 481 NPREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVS 540
           NPR+AFFAKK +++I   VG+VCGEL CP+PPGIPVTIPGEVI+EEV D+LLHLK KG S
Sbjct: 487 NPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGAS 546

Query: 541 ITGASDPQLSSLVVCNV 557
           I+GASDPQLSSL+VCNV
Sbjct: 547 ISGASDPQLSSLLVCNV 557

BLAST of Lcy03g017480 vs. ExPASy TrEMBL
Match: A0A6J1E1Q8 (uncharacterized protein LOC111429785 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429785 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 5.3e-240
Identity = 421/557 (75.58%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 1   MASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPV 60
           M S  LS S    +HFR  ++WS  P+RRK  F + QETSIV+  NNQD SSK+Q     
Sbjct: 2   MTSSPLSSSIISNHHFRLGHDWSRRPQRRKSCFSIRQETSIVKPKNNQDGSSKKQTSTKA 61

Query: 61  IRDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFS 120
           I +SP SQEGS+  PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIGLKPFMHD  
Sbjct: 62  IGESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGLKPFMHDLP 121

Query: 121 QVPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPR 180
           ++PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPR
Sbjct: 122 ELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPR 181

Query: 181 NSHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTS 240
           NSH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV +AIED EME QK SAV VTS
Sbjct: 182 NSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDRAIEDLEMEGQKASAVLVTS 241

Query: 241 PTYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVL 300
           PTYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVL
Sbjct: 242 PTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVL 301

Query: 301 CSLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 360
           CSLTQSSMLH+SGNIIDRE VCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AI
Sbjct: 302 CSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAI 361

Query: 361 DLANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVT 420
           DLA QAK K+NKIS IS+LEFP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+  ++KNH + 
Sbjct: 362 DLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADATIYKNHNIV 421

Query: 421 CELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGM 480
           CEL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK ++ APF ++KI +
Sbjct: 422 CELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKFSVSAPFPNVKISL 481

Query: 481 NPREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVS 540
           NPR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG S
Sbjct: 482 NPRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVLDYLLHLKSKGAS 541

Query: 541 ITGASDPQLSSLVVCNV 557
           I+GASDP+L SL+VCNV
Sbjct: 542 ISGASDPKLYSLLVCNV 558

BLAST of Lcy03g017480 vs. ExPASy TrEMBL
Match: A0A6J1JDD3 (uncharacterized protein LOC111484796 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484796 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.5e-239
Identity = 418/557 (75.04%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 1   MASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPV 60
           M S  LS S +  +HFR  ++WS  P+RRK  F + QETSIV+  NNQD  SK+Q     
Sbjct: 2   MTSSPLSSSISLNHHFRLGHDWSRRPQRRKSCFSIRQETSIVKPKNNQDGPSKKQTSTKA 61

Query: 61  IRDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFS 120
           I +SP SQEGS   PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIGLKPFMHD  
Sbjct: 62  IGESPISQEGSRTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGLKPFMHDLP 121

Query: 121 QVPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPR 180
           ++PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPR
Sbjct: 122 ELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPR 181

Query: 181 NSHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTS 240
           NSH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV + I+D EME QK SAV VTS
Sbjct: 182 NSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDREIKDLEMEGQKASAVLVTS 241

Query: 241 PTYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVL 300
           PTYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVL
Sbjct: 242 PTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVL 301

Query: 301 CSLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 360
           CSLTQSSMLH+SGNIIDRE +CRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AI
Sbjct: 302 CSLTQSSMLHMSGNIIDREMICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAI 361

Query: 361 DLANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVT 420
           DLA QAKRK+NK S IS+L+FP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+E ++KNH + 
Sbjct: 362 DLAYQAKRKMNKTSGISILKFPMFSNFPAIDPLRLTIGFQQLGLSGYEADETIYKNHNIV 421

Query: 421 CELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGM 480
           CEL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK ++ APF ++KI +
Sbjct: 422 CELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKFSVSAPFPNVKINL 481

Query: 481 NPREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVS 540
           NPR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG S
Sbjct: 482 NPRDAFFSKKRRENIKECVGKVCGELVCPYPPGIPVMIPGEIISEEVLDYLLHLKSKGAS 541

Query: 541 ITGASDPQLSSLVVCNV 557
           I+GASDP+LSSL+VCNV
Sbjct: 542 ISGASDPKLSSLLVCNV 558

BLAST of Lcy03g017480 vs. ExPASy TrEMBL
Match: A0A6J1E647 (uncharacterized protein LOC111429785 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429785 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 2.0e-239
Identity = 419/556 (75.36%), Postives = 475/556 (85.43%), Query Frame = 0

Query: 2   ASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPVI 61
           +SP  S   +  +HFR  ++WS  P+RRK  F + QETSIV+  NNQD SSK+Q     I
Sbjct: 4   SSPLSSSIISQNHHFRLGHDWSRRPQRRKSCFSIRQETSIVKPKNNQDGSSKKQTSTKAI 63

Query: 62  RDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQ 121
            +SP SQEGS+  PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIGLKPFMHD  +
Sbjct: 64  GESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGLKPFMHDLPE 123

Query: 122 VPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRN 181
           +PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPRN
Sbjct: 124 LPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRN 183

Query: 182 SHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSP 241
           SH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV +AIED EME QK SAV VTSP
Sbjct: 184 SHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDRAIEDLEMEGQKASAVLVTSP 243

Query: 242 TYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLC 301
           TYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVLC
Sbjct: 244 TYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVLC 303

Query: 302 SLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAID 361
           SLTQSSMLH+SGNIIDRE VCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AID
Sbjct: 304 SLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAID 363

Query: 362 LANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVTC 421
           LA QAK K+NKIS IS+LEFP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+  ++KNH + C
Sbjct: 364 LAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADATIYKNHNIVC 423

Query: 422 ELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMN 481
           EL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK ++ APF ++KI +N
Sbjct: 424 ELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKFSVSAPFPNVKISLN 483

Query: 482 PREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSI 541
           PR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG SI
Sbjct: 484 PRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVLDYLLHLKSKGASI 543

Query: 542 TGASDPQLSSLVVCNV 557
           +GASDP+L SL+VCNV
Sbjct: 544 SGASDPKLYSLLVCNV 559

BLAST of Lcy03g017480 vs. NCBI nr
Match: XP_022134581.1 (uncharacterized protein LOC111006813 [Momordica charantia])

HSP 1 Score: 869.4 bits (2245), Expect = 1.7e-248
Identity = 433/527 (82.16%), Postives = 476/527 (90.32%), Query Frame = 0

Query: 32  YFKVLQETSIVET--NNNQDRSSKEQNDKPVIRDSPRSQEGSNPPLIRALKASAEQNTAD 91
           + ++     ++ET  NNNQDRSSK+QN     RDSPR QE S+PPLIRALK SAEQN AD
Sbjct: 23  FVRLYSSIFLLETKPNNNQDRSSKKQNQN--ARDSPRFQESSDPPLIRALKVSAEQNAAD 82

Query: 92  FHFPGHKRGQAAPSSITQLIGLKPFMHDFSQVPELDNLFRPEGPILEAQQQAAKLFGASE 151
           FHFPGH RG+AAPSS+TQLIGLKPFMHD  Q PELDNLFRPEGPILEA Q+AA+LFGASE
Sbjct: 83  FHFPGHNRGRAAPSSLTQLIGLKPFMHDLPQFPELDNLFRPEGPILEALQEAAQLFGASE 142

Query: 152 TWFLVGGTTSGIQAAIMATCSPGEYIILPRNSHASAISALVFTGANPIYIIPEYDSNWDI 211
           TWFLVGGTTSGIQAAIMATCSPGE+IILPRNSH SAISALVF+GA P+YI+PEYDSNWDI
Sbjct: 143 TWFLVGGTTSGIQAAIMATCSPGEHIILPRNSHVSAISALVFSGAKPVYIMPEYDSNWDI 202

Query: 212 AGGVTPSQVVKAIEDSEMECQKVSAVFVTSPTYHGICSNLSEISQICHSKGIPLIVDEAH 271
           AGGVTPSQV +AI+ SEME +KVSAVFVTSPTYHGICSNLSEIS+ICHS GIPLIVDEAH
Sbjct: 203 AGGVTPSQVSRAIQISEMEGKKVSAVFVTSPTYHGICSNLSEISKICHSNGIPLIVDEAH 262

Query: 272 GAHFGFQPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHLSGNIIDRERVCRCLQTLQS 331
           GAHFGF PQLPHSALQQGADLVVQSTHKVLCSLTQSSMLH+SGN +DRERVCR LQ+LQS
Sbjct: 263 GAHFGFHPQLPHSALQQGADLVVQSTHKVLCSLTQSSMLHMSGNTVDRERVCRSLQSLQS 322

Query: 332 TSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKRKINKISDISVLEFPIFSNFPAI 391
           TSPSYLLLASLDAARAQ+SDNPD+IFNKAIDLANQAK+KI KIS IS+ E PIF NFPAI
Sbjct: 323 TSPSYLLLASLDAARAQISDNPDEIFNKAIDLANQAKQKITKISGISIPELPIFPNFPAI 382

Query: 392 DPLRLTIGFQKLGLSGYEANEILHKNHGVTCELIGTQSITIVINLGICEHDIQRLVSGLE 451
           DPLRLTIGFQ+LGLSGY+A+EILHKNHG+ CELIGTQS+T+VINLGICE DIQRLVSGLE
Sbjct: 383 DPLRLTIGFQQLGLSGYKADEILHKNHGLICELIGTQSVTLVINLGICERDIQRLVSGLE 442

Query: 452 DVSSFGPILGIEQRSKLNIFAPFTDIKIGMNPREAFFAKKGKQSITRSVGEVCGELTCPF 511
           DVSS+G IL IE RSK+ + APFTDI+I MNPR+AFFA+K +++I  SVGEVCGEL CP+
Sbjct: 443 DVSSYGSILRIEGRSKVIVSAPFTDIRIKMNPRDAFFARKRRENIRESVGEVCGELICPY 502

Query: 512 PPGIPVTIPGEVITEEVRDFLLHLKRKGVSITGASDPQLSSLVVCNV 557
           PPGIPVTIPGEVITEEVRDFLLHLK KG  ITGASDPQLSS+VVCNV
Sbjct: 503 PPGIPVTIPGEVITEEVRDFLLHLKNKGACITGASDPQLSSIVVCNV 547

BLAST of Lcy03g017480 vs. NCBI nr
Match: XP_022134879.1 (uncharacterized protein LOC111007031 [Momordica charantia])

HSP 1 Score: 858.6 bits (2217), Expect = 3.0e-245
Identity = 442/557 (79.35%), Postives = 480/557 (86.18%), Query Frame = 0

Query: 1   MASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQND-KP 60
           MAS  LS S +  +HFR A+N     R RK  F V QETSIVE  NNQDRSSK+Q+  K 
Sbjct: 7   MASSPLSSSTSLNHHFRSAHN-----RGRKSCFSVRQETSIVEPKNNQDRSSKKQSTAKI 66

Query: 61  VIRDSPRSQEGSNPPLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFS 120
            I +SP S+E S PPL+ ALK SAEQN A FHFPGH RG+AAPSS TQLIGLKPFMHD  
Sbjct: 67  AISESPISKE-SCPPLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLP 126

Query: 121 QVPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPR 180
           ++PELDNLF PEGPILEAQQQAAKLFGA ETWFLVGGTT GIQ AIMATCSPGE+II+PR
Sbjct: 127 ELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPR 186

Query: 181 NSHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTS 240
           NSH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV KAI+DSEME  KVSAVFVTS
Sbjct: 187 NSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPSQVDKAIKDSEMEGHKVSAVFVTS 246

Query: 241 PTYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVL 300
           PTYHGICSNLSEISQICHS GIPLIVDEAHGAHFGFQPQ+PHSALQQG DLV QSTHKVL
Sbjct: 247 PTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVL 306

Query: 301 CSLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 360
            SLTQSSMLH+SGNI+DRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI
Sbjct: 307 SSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 366

Query: 361 DLANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVT 420
           DLANQAK KINKIS IS+LE PIFSN PAIDPLRLTIGFQ+LGLSGYEA+EILHKNH + 
Sbjct: 367 DLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTIGFQQLGLSGYEADEILHKNHDIV 426

Query: 421 CELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGM 480
           CEL+GTQSIT VINLG CE DI+RLVSG+EDVSS   IL IE RSKL+  APF DIKI +
Sbjct: 427 CELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIVL 486

Query: 481 NPREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVS 540
           NPR+AFFAKK +++I   VG+VCGEL CP+PPGIPVTIPGEVI+EEV D+LLHLK KG S
Sbjct: 487 NPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGAS 546

Query: 541 ITGASDPQLSSLVVCNV 557
           I+GASDPQLSSL+VCNV
Sbjct: 547 ISGASDPQLSSLLVCNV 557

BLAST of Lcy03g017480 vs. NCBI nr
Match: XP_023515532.1 (uncharacterized protein LOC111779660 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.7 bits (2181), Expect = 4.4e-241
Identity = 423/557 (75.94%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 1   MASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPV 60
           M S  LS S    +HFR  ++WS  P+RRK  F + QETSIV+  NNQD SSK+Q     
Sbjct: 2   MTSSPLSSSIISNHHFRLGHDWSRRPKRRKSCFSIRQETSIVKPKNNQDGSSKKQTSTKA 61

Query: 61  IRDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFS 120
           I +SP SQEGS+  PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIG KPFMHD  
Sbjct: 62  IGESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGFKPFMHDLP 121

Query: 121 QVPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPR 180
           ++PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPR
Sbjct: 122 ELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPR 181

Query: 181 NSHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTS 240
           NSH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV +AIED EME QK SAV VTS
Sbjct: 182 NSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDRAIEDLEMEGQKASAVLVTS 241

Query: 241 PTYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVL 300
           PTYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVL
Sbjct: 242 PTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVL 301

Query: 301 CSLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAI 360
           CSLTQSSMLH+SGNIIDRE VCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AI
Sbjct: 302 CSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAI 361

Query: 361 DLANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVT 420
           DLA QAK KINKIS IS+LEFP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+E ++KNH + 
Sbjct: 362 DLAYQAKSKINKISGISILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADETIYKNHNIV 421

Query: 421 CELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGM 480
           CEL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK ++ APF D+K  +
Sbjct: 422 CELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKFSVSAPFPDVKTSL 481

Query: 481 NPREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVS 540
           NPR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG S
Sbjct: 482 NPRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVLDYLLHLKSKGAS 541

Query: 541 ITGASDPQLSSLVVCNV 557
           I+GASDP+LSSL+VCNV
Sbjct: 542 ISGASDPKLSSLLVCNV 558

BLAST of Lcy03g017480 vs. NCBI nr
Match: XP_023515530.1 (uncharacterized protein LOC111779660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515531.1 uncharacterized protein LOC111779660 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842.8 bits (2176), Expect = 1.7e-240
Identity = 421/556 (75.72%), Postives = 475/556 (85.43%), Query Frame = 0

Query: 2   ASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPVI 61
           +SP  S   +  +HFR  ++WS  P+RRK  F + QETSIV+  NNQD SSK+Q     I
Sbjct: 4   SSPLSSSIISQNHHFRLGHDWSRRPKRRKSCFSIRQETSIVKPKNNQDGSSKKQTSTKAI 63

Query: 62  RDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQ 121
            +SP SQEGS+  PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIG KPFMHD  +
Sbjct: 64  GESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGFKPFMHDLPE 123

Query: 122 VPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRN 181
           +PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPRN
Sbjct: 124 LPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRN 183

Query: 182 SHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSP 241
           SH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV +AIED EME QK SAV VTSP
Sbjct: 184 SHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDRAIEDLEMEGQKASAVLVTSP 243

Query: 242 TYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLC 301
           TYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVLC
Sbjct: 244 TYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVLC 303

Query: 302 SLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAID 361
           SLTQSSMLH+SGNIIDRE VCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AID
Sbjct: 304 SLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAID 363

Query: 362 LANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVTC 421
           LA QAK KINKIS IS+LEFP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+E ++KNH + C
Sbjct: 364 LAYQAKSKINKISGISILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADETIYKNHNIVC 423

Query: 422 ELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMN 481
           EL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK ++ APF D+K  +N
Sbjct: 424 ELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKFSVSAPFPDVKTSLN 483

Query: 482 PREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSI 541
           PR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG SI
Sbjct: 484 PRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVLDYLLHLKSKGASI 543

Query: 542 TGASDPQLSSLVVCNV 557
           +GASDP+LSSL+VCNV
Sbjct: 544 SGASDPKLSSLLVCNV 559

BLAST of Lcy03g017480 vs. NCBI nr
Match: KAG6589654.1 (hypothetical protein SDJN03_15077, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 841.3 bits (2172), Expect = 4.9e-240
Identity = 420/556 (75.54%), Postives = 476/556 (85.61%), Query Frame = 0

Query: 2   ASPALSYSGNPVNHFRRANNWSLGPRRRKPYFKVLQETSIVETNNNQDRSSKEQNDKPVI 61
           +SP  S      +HFR  ++WS  P+RRK  F + QETSIV+  NNQD SSK+Q     I
Sbjct: 4   SSPLSSSIITQNHHFRVGHDWSRRPQRRKSCFSIRQETSIVKPKNNQDGSSKKQTSTKAI 63

Query: 62  RDSPRSQEGSNP-PLIRALKASAEQNTADFHFPGHKRGQAAPSSITQLIGLKPFMHDFSQ 121
            +SP SQEGS+  PL+ ALK SAE++ A FHFPGH  G+AAPSS TQLIGLKPFMHD  +
Sbjct: 64  GESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHNGGRAAPSSFTQLIGLKPFMHDLPE 123

Query: 122 VPELDNLFRPEGPILEAQQQAAKLFGASETWFLVGGTTSGIQAAIMATCSPGEYIILPRN 181
           +PELDNLF PEGPILEAQQQAAKLFGASETWFLVGGTT GIQAAIMATCSPG++IILPRN
Sbjct: 124 LPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRN 183

Query: 182 SHASAISALVFTGANPIYIIPEYDSNWDIAGGVTPSQVVKAIEDSEMECQKVSAVFVTSP 241
           SH S ISALV +GA P YI+PEYDSNWDIAGGVTPSQV +AIED EME QK SAV VTSP
Sbjct: 184 SHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPSQVDRAIEDLEMEGQKASAVLVTSP 243

Query: 242 TYHGICSNLSEISQICHSKGIPLIVDEAHGAHFGFQPQLPHSALQQGADLVVQSTHKVLC 301
           TYHGICS+L EISQICH+KGIPLIVDEAHGAHFGFQPQLP SALQQGADL VQSTHKVLC
Sbjct: 244 TYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPLSALQQGADLAVQSTHKVLC 303

Query: 302 SLTQSSMLHLSGNIIDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAID 361
           SLTQSSMLH+SGNIIDRE VCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFN+AID
Sbjct: 304 SLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAID 363

Query: 362 LANQAKRKINKISDISVLEFPIFSNFPAIDPLRLTIGFQKLGLSGYEANEILHKNHGVTC 421
           LA QAK K+NKIS IS+LEFP+FSNFPAIDPLRLTIGFQ+LGLSGYEA+E ++KNH + C
Sbjct: 364 LAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADETIYKNHNIVC 423

Query: 422 ELIGTQSITIVINLGICEHDIQRLVSGLEDVSSFGPILGIEQRSKLNIFAPFTDIKIGMN 481
           EL+G QSIT VINLG CE DI+RLVSG++DVSSF  IL IE RSK+ + APF ++KI +N
Sbjct: 424 ELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIEGRSKVTVSAPFPNVKISLN 483

Query: 482 PREAFFAKKGKQSITRSVGEVCGELTCPFPPGIPVTIPGEVITEEVRDFLLHLKRKGVSI 541
           PR+AFF+KK +++I   VG+VCGEL CP+PPGIPV IPGE+I+EEV D+LLHLK KG S+
Sbjct: 484 PRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVLDYLLHLKSKGASL 543

Query: 542 TGASDPQLSSLVVCNV 557
           +GASDP+LSSL+VCNV
Sbjct: 544 SGASDPKLSSLLVCNV 559