Lcy03g004230 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g004230
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Reverse transcriptase domain-containing protein
Location	Chr03: 20501173 .. 20501865 (-)
RNA-Seq Expression	Lcy03g004230
Synteny	Lcy03g004230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCAGCGGAAGATTATAGAATTAGAAAGAAGAAAGGTTTGTTGCTCAAATTGGACCTTGAAAAGGCTTATGATATGGTTAATTGGCAGTTTCTTGATGAGATTTTAGCTTTGAAGGGCTTCGGTCAGATTTGGAGAAGGTGGATAGCCGGCTGTCTTAGAAACACTAACTTTTCCATTATGATCAATGGTAGACCGAGAGGTAAGATTGTGGCTTCTAGAGGGCTTCGGCAAGGGGACCCTCTTTCCCCTTTTCTCTTCACTATTGTTGGAGATGCGGTTTGTAAGTTTGTTAAATTTTGCCTTGAGAGAAAGATCCTTAAGGGATGGGAAATAGGGGTGAATAAAGTGGAGCTGTCCATGTTGCAATATGCCGATGACACCTTGATTTTTTGTCCGAACAGTGAAGCTGACTTACAGGGCTGGAAGGAGATTATTTTACTGATTATGGAAGGCTCGGGTCTAAGACTTAATCTCCTGAAGTCTTCCCTTATAGGCATCAAAGTGGATAAAGATGAGCTTGATTTGTGGGCCACCAAGTTTGGATGCCACTCTAATGAGCTTCCTTTTAATTACCTCGGTTTTCAACTAGGAGGTAATTATCATAGGCTTTCAGCAGAAGCTAGACAAATGGCAGAAATTCCCCATTTCAAAAGGAGGAAGACTCACCTTGGCACAAGCTGTCCTTAA

mRNA sequence

ATGGAAGCAGCGGAAGATTATAGAATTAGAAAGAAGAAAGGTTTGTTGCTCAAATTGGACCTTGAAAAGGCTTATGATATGGTTAATTGGCAGTTTCTTGATGAGATTTTAGCTTTGAAGGGCTTCGGTCAGATTTGGAGAAGGTGGATAGCCGGCTGTCTTAGAAACACTAACTTTTCCATTATGATCAATGGTAGACCGAGAGGTAAGATTGTGGCTTCTAGAGGGCTTCGGCAAGGGGACCCTCTTTCCCCTTTTCTCTTCACTATTGTTGGAGATGCGGTTTGTAAGTTTGTTAAATTTTGCCTTGAGAGAAAGATCCTTAAGGGATGGGAAATAGGGGTGAATAAAGTGGAGCTGTCCATGTTGCAATATGCCGATGACACCTTGATTTTTTGTCCGAACAGTGAAGCTGACTTACAGGGCTGGAAGGAGATTATTTTACTGATTATGGAAGGCTCGGGTCTAAGACTTAATCTCCTGAAGTCTTCCCTTATAGGCATCAAAGTGGATAAAGATGAGCTTGATTTGTGGGCCACCAAGTTTGGATGCCACTCTAATGAGCTTCCTTTTAATTACCTCGGTTTTCAACTAGGAGGTAATTATCATAGGCTTTCAGCAGAAGCTAGACAAATGGCAGAAATTCCCCATTTCAAAAGGAGGAAGACTCACCTTGGCACAAGCTGTCCTTAA

Coding sequence (CDS)

ATGGAAGCAGCGGAAGATTATAGAATTAGAAAGAAGAAAGGTTTGTTGCTCAAATTGGACCTTGAAAAGGCTTATGATATGGTTAATTGGCAGTTTCTTGATGAGATTTTAGCTTTGAAGGGCTTCGGTCAGATTTGGAGAAGGTGGATAGCCGGCTGTCTTAGAAACACTAACTTTTCCATTATGATCAATGGTAGACCGAGAGGTAAGATTGTGGCTTCTAGAGGGCTTCGGCAAGGGGACCCTCTTTCCCCTTTTCTCTTCACTATTGTTGGAGATGCGGTTTGTAAGTTTGTTAAATTTTGCCTTGAGAGAAAGATCCTTAAGGGATGGGAAATAGGGGTGAATAAAGTGGAGCTGTCCATGTTGCAATATGCCGATGACACCTTGATTTTTTGTCCGAACAGTGAAGCTGACTTACAGGGCTGGAAGGAGATTATTTTACTGATTATGGAAGGCTCGGGTCTAAGACTTAATCTCCTGAAGTCTTCCCTTATAGGCATCAAAGTGGATAAAGATGAGCTTGATTTGTGGGCCACCAAGTTTGGATGCCACTCTAATGAGCTTCCTTTTAATTACCTCGGTTTTCAACTAGGAGGTAATTATCATAGGCTTTCAGCAGAAGCTAGACAAATGGCAGAAATTCCCCATTTCAAAAGGAGGAAGACTCACCTTGGCACAAGCTGTCCTTAA

Protein sequence

MEAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATKFGCHSNELPFNYLGFQLGGNYHRLSAEARQMAEIPHFKRRKTHLGTSCP

Homology

[Close]

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-13
Identity = 44/156 (28.21%), Postives = 82/156 (52.56%), Query Frame = 0


Query: 8   RIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRP 67
           R + K  +++ +D EKA+D +   F+ + L   G   ++ + I         +I++NG+ 
Sbjct: 588 RAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQK 647

Query: 68  RGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYAD 127
                   G RQG PLSP LF IV + + + ++   + K +KG ++G  +V+LS+  +AD
Sbjct: 648 LEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIR---QEKEIKGIQLGKEEVKLSL--FAD 707

Query: 128 DTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKS 164
           D +++  N     Q   ++I    + SG ++N+ KS
Sbjct: 708 DMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKS 738

[Close]

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 44/156 (28.21%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 8   RIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRP 67
           +++ K  +++ LD EKA+D +   F+ ++L   G    +   I         +I +NG  
Sbjct: 595 KLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEK 654

Query: 68  RGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYAD 127
              I    G RQG PLSP+LF IV + + + ++   ++K +KG +IG  +V++S+L  AD
Sbjct: 655 LEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIR---QQKEIKGIQIGKEEVKISLL--AD 714

Query: 128 DTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKS 164
           D +++  + +   +    +I    E  G ++N  KS
Sbjct: 715 DMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKS 745

[Close]

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.6e-12
Identity = 45/156 (28.85%), Postives = 76/156 (48.72%), Query Frame = 0


Query: 8   RIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRP 67
           +++ K  ++L +D EKA+D +   F+   L   G    + + I         +I++NG  
Sbjct: 587 KLKNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVK 646

Query: 68  RGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYAD 127
                   G RQG PLSP LF IV + +   ++   E K +KG  IG  +++LS+  +AD
Sbjct: 647 LKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEEIKLSL--FAD 706

Query: 128 DTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKS 164
           D +++  N+        E+I      SG ++N  KS
Sbjct: 707 DMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKS 737

[Close]

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 0


Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
           +ING P+G +  SRGLRQGDPLSP+LF +  + +    +   E+  L G  +  N   ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 122 MLQYADDT 130
            L +ADDT
Sbjct: 73  HLLFADDT 80

[Close]

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-07
Identity = 48/162 (29.63%), Postives = 76/162 (46.91%), Query Frame = 0


Query: 8   RIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMIN-GR 67
           R ++K   ++ LD+ KA+D V+   +   L   G  +    +I G L ++  +I +  G 
Sbjct: 131 REQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPGS 190

Query: 68  PRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYA 127
              KI   RG++QGDPLSPFLF  V D +       L+     G  IG  K+   +L +A
Sbjct: 191 QTRKICIRRGVKQGDPLSPFLFNAVLDELL----CSLQSTPGIGGTIGEEKI--PVLAFA 250

Query: 128 DDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGI 169
           DD L+   +++  L      +       G+ LN  KS  I +
Sbjct: 251 DD-LLLLEDNDVLLPTTLATVANFFRLRGMSLNAKKSVSISV 285

[Close]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL
Match: A0A6J1E196 (uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025019 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 4.1e-53
Identity = 99/188 (52.66%), Postives = 133/188 (70.74%), Query Frame = 0


Query: 27  MVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRPRGKIVASRGLRQGDPLSPF 86
           MV+W FLD +L  KGFG+ WR WI GC+ + NFS+ INGRPRGKI A+RGLRQGDPLSPF
Sbjct: 1   MVDWCFLDVVLLQKGFGKRWRMWIKGCVSSANFSVFINGRPRGKIFATRGLRQGDPLSPF 60

Query: 87  LFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYADDTLIFCPNSEADLQGWKEI 146
           LFT++GD+  + V FC ER+I++G+ +G  ++ +S L+YADDT+IFCP  + +L  W  +
Sbjct: 61  LFTLIGDSFSRLVNFCCERRIIQGFAVGRERIVVSHLRYADDTIIFCPAYKGNLDRWWAL 120

Query: 147 ILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATKFGCHSNELPFNYLGFQLGGNYHRLS 206
           I L ++GSGLR+NL K+SLIGI + ++E      K GC    + F YLG  LGGN HR+ 
Sbjct: 121 ISLFIKGSGLRINLAKTSLIGINIGENEATSKVDKIGCRVERMSFRYLGLPLGGN-HRVI 180

Query: 207 AEARQMAE 215
              R + E
Sbjct: 181 VFWRHLVE 187

[Close]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL
Match: A0A803QQM3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 8.0e-49
Identity = 101/205 (49.27%), Postives = 136/205 (66.34%), Query Frame = 0


Query: 2    EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
            EA EDYR R KKG++LK+D EKAYD V+W FLD ++  KGFG+ W +WI GC+  T+FSI
Sbjct: 1486 EAVEDYRSRGKKGIVLKIDFEKAYDRVDWGFLDLVMRKKGFGERWTKWIRGCVSTTSFSI 1545

Query: 62   MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
             INGR RGK   SRGLRQ DPLSPFLFT++ D + + V   ++ + L G++IG + ++LS
Sbjct: 1546 FINGRVRGKFNGSRGLRQVDPLSPFLFTLIADVLGRMVDKAIDTESLSGFQIGKDDIQLS 1605

Query: 122  MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
             LQ+ADDTL F    EA LQ   +I+      SGL++NL KS L+G+ +D+D +   A +
Sbjct: 1606 HLQFADDTLFFV-KDEASLQKLVKIVEAFCGISGLKVNLNKSQLLGVCMDEDAVAQSAIQ 1665

Query: 182  FGCHSNELPFNYLGFQLGGNYHRLS 207
             GC     P  YLG  LGG+  + S
Sbjct: 1666 IGCEVGRWPMTYLGMPLGGSPRKRS 1689

[Close]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL
Match: A0A803QEA6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.0e-48
Identity = 101/205 (49.27%), Postives = 135/205 (65.85%), Query Frame = 0


Query: 2    EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
            EA EDYR R +KG +LK+D EKAYD V+W FLD +L  KGFG+ WR+WI GC+ +T+FSI
Sbjct: 913  EAVEDYRSRGRKGFVLKIDFEKAYDRVDWGFLDMVLRKKGFGERWRKWIRGCVSSTSFSI 972

Query: 62   MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
             INGR RGK   SRGLRQGDPLSPFLFT++ D + + V   +E + L G++IG + + LS
Sbjct: 973  FINGRVRGKFNGSRGLRQGDPLSPFLFTMIADVLGRMVDKAIETESLTGFQIGKDDIRLS 1032

Query: 122  MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
             LQ+ADDTL F    E  LQ   +++      SGL++NL KS L+GI ++++ +   A  
Sbjct: 1033 HLQFADDTLFFV-KDEVSLQKLVKVVKAFCGISGLKVNLNKSQLLGICMNEEAVAQSAIL 1092

Query: 182  FGCHSNELPFNYLGFQLGGNYHRLS 207
             GC     P  YLG  LGG+  + S
Sbjct: 1093 IGCEVGRWPMTYLGMSLGGSPRKRS 1116

[Close]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL
Match: A0A803P8A0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.8e-48
Identity = 101/200 (50.50%), Postives = 131/200 (65.50%), Query Frame = 0


Query: 2   EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
           EA EDYR R KKG +LK+D EKAYD V+W FLD +L  KGFG+ WR+WI GC+ +T+FSI
Sbjct: 563 EAVEDYRSRGKKGFVLKIDFEKAYDRVDWGFLDLVLRKKGFGERWRKWIRGCVSSTSFSI 622

Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
            +NGR RGK   SRGLRQGDPLSPFLFT+V D + + V   +E +   G++IG + + LS
Sbjct: 623 FVNGRVRGKFHGSRGLRQGDPLSPFLFTLVADVLGRMVDKAVETEAFSGFQIGKDNIRLS 682

Query: 122 MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
            LQ+ADDTL F    E  LQ   +I+      SGL++NL KS L+GI +  + +   A  
Sbjct: 683 HLQFADDTLFFV-KDEDSLQKLVKIVEAFCGISGLKVNLNKSQLLGICLSDEAVAQGANL 742

Query: 182 FGCHSNELPFNYLGFQLGGN 202
            GC   + P  YLG  LGG+
Sbjct: 743 IGCEVGKWPMTYLGMPLGGS 761

[Close]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL
Match: A0A803QI00 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 6.7e-48
Identity = 97/200 (48.50%), Postives = 133/200 (66.50%), Query Frame = 0


Query: 2    EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
            E  ED+R R KKG + K+DLEKAYD V+W FLD +L  KGFG++WR+WI GC+ +T+FS+
Sbjct: 1462 ETVEDFRSRGKKGFVFKIDLEKAYDRVDWDFLDLVLKEKGFGEVWRKWIRGCVSSTSFSL 1521

Query: 62   MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
            +INGR RGK   SRGLRQGDPLSPFLFT+V D + + V    +     G+++G + +++S
Sbjct: 1522 LINGRVRGKFRGSRGLRQGDPLSPFLFTLVVDVLGRLVDKAAQSDTFSGFQVGKDNIQIS 1581

Query: 122  MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
             LQ+ADDTL F    EA L+   EI+      SGL++NL KS L+GI ++++ +   A  
Sbjct: 1582 HLQFADDTLFFV-KDEASLRKLVEIVEAFCGISGLKVNLNKSQLLGISLEEEVVAQNAEI 1641

Query: 182  FGCHSNELPFNYLGFQLGGN 202
             GC     P  YLG  LGG+
Sbjct: 1642 IGCEVGTWPMTYLGMPLGGS 1660

[Close]

BLAST of Lcy03g004230 vs. NCBI nr
Match: XP_022158566.1 (uncharacterized protein LOC111025019 [Momordica charantia])

HSP 1 Score: 218.0 bits (554), Expect = 8.4e-53
Identity = 99/188 (52.66%), Postives = 133/188 (70.74%), Query Frame = 0


Query: 27  MVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSIMINGRPRGKIVASRGLRQGDPLSPF 86
           MV+W FLD +L  KGFG+ WR WI GC+ + NFS+ INGRPRGKI A+RGLRQGDPLSPF
Sbjct: 1   MVDWCFLDVVLLQKGFGKRWRMWIKGCVSSANFSVFINGRPRGKIFATRGLRQGDPLSPF 60

Query: 87  LFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELSMLQYADDTLIFCPNSEADLQGWKEI 146
           LFT++GD+  + V FC ER+I++G+ +G  ++ +S L+YADDT+IFCP  + +L  W  +
Sbjct: 61  LFTLIGDSFSRLVNFCCERRIIQGFAVGRERIVVSHLRYADDTIIFCPAYKGNLDRWWAL 120

Query: 147 ILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATKFGCHSNELPFNYLGFQLGGNYHRLS 206
           I L ++GSGLR+NL K+SLIGI + ++E      K GC    + F YLG  LGGN HR+ 
Sbjct: 121 ISLFIKGSGLRINLAKTSLIGINIGENEATSKVDKIGCRVERMSFRYLGLPLGGN-HRVI 180

Query: 207 AEARQMAE 215
              R + E
Sbjct: 181 VFWRHLVE 187

[Close]

BLAST of Lcy03g004230 vs. NCBI nr
Match: XP_009335445.1 (PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri])

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-47
Identity = 97/199 (48.74%), Postives = 128/199 (64.32%), Query Frame = 0


Query: 2   EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
           E  E+ R +K++GL+ K+D EKAYD V W+FLDEIL  KGFG  WR+W+ GC  + NFSI
Sbjct: 383 ELVEEVRQKKEEGLMFKIDFEKAYDHVEWRFLDEILQRKGFGNRWRKWVQGCQNSANFSI 442

Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
           +INGRP+GK  ASR LRQGDPLSPFLFT+V D + + ++   E  ++KG  IG  +VE+S
Sbjct: 443 LINGRPKGKFHASRRLRQGDPLSPFLFTLVVDVLSRLMEKAHENHLIKGLGIGQERVEIS 502

Query: 122 MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
            LQ+ADDT+ F    E       E++ L    SGL++N  K S+ GI  D ++L   A  
Sbjct: 503 HLQFADDTIFFLAEDEDVWNNLLEVLNLFCTISGLKINKAKCSMAGINSDCEKLKGMADS 562

Query: 182 FGCHSNELPFNYLGFQLGG 201
           +GC     P  YLG  LGG
Sbjct: 563 WGCEVGSWPIKYLGLPLGG 581

[Close]

BLAST of Lcy03g004230 vs. NCBI nr
Match: RVX20162.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 197.6 bits (501), Expect = 1.2e-46
Identity = 91/200 (45.50%), Postives = 134/200 (67.00%), Query Frame = 0


Query: 2   EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
           E  ++ R   ++G++ K+D EKAYD V W FLD +L  KGF   WR+W+ GCL + +++I
Sbjct: 723 EIVDEKRQSGEEGVVFKIDFEKAYDHVKWDFLDHVLEKKGFSPKWRKWMRGCLSSVSYAI 782

Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
           ++N   +G + ASRGLRQGDPLSPFLFTIV D + + +    ER +L+G+ +G N+  +S
Sbjct: 783 LVNDNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRMLLKAEERSLLEGFRVGRNRCRVS 842

Query: 122 MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
            LQ+ADDT+ F  +SE +LQ  K ++L+  + SGL++NL KS+L GI +D+D L   A  
Sbjct: 843 HLQFADDTIFFANSSEEELQTLKSLLLVFGQVSGLKVNLDKSNLFGINLDQDHLSRLALL 902

Query: 182 FGCHSNELPFNYLGFQLGGN 202
             C +++ P  YLG  LGGN
Sbjct: 903 LDCKASDWPILYLGLPLGGN 922

[Close]

BLAST of Lcy03g004230 vs. NCBI nr
Match: RVW91328.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 196.4 bits (498), Expect = 2.6e-46
Identity = 88/200 (44.00%), Postives = 137/200 (68.50%), Query Frame = 0


Query: 2   EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
           E  ++ +   ++G++ K+D EKAYD VNW FLD++L +KGF   WR W+ GCL + +++I
Sbjct: 572 EIVDEKKRSGEEGVVFKIDFEKAYDHVNWDFLDQVLEMKGFSPRWRSWMRGCLSSVSYAI 631

Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
           ++NG  +G + A+RGLRQGDPLSPFLFTIV D + + +    ER +L+G+ +G N+  +S
Sbjct: 632 LVNGNAKGWVKAARGLRQGDPLSPFLFTIVADVLSRMLLKAEERNLLEGFRVGRNRCRVS 691

Query: 122 MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
            LQ+ADDT++F    E ++Q  K ++L+  + SGL++NL KS+L GI +D++ L   A+ 
Sbjct: 692 HLQFADDTILFASPREDEVQTLKSLLLVFGQISGLKVNLDKSNLFGINLDQNHLSRLASL 751

Query: 182 FGCHSNELPFNYLGFQLGGN 202
             C +++ P  YLG  LGGN
Sbjct: 752 LDCKASDWPILYLGLPLGGN 771

[Close]

BLAST of Lcy03g004230 vs. NCBI nr
Match: RVX14115.1 (Protein SWEETIE [Vitis vinifera])

HSP 1 Score: 196.4 bits (498), Expect = 2.6e-46
Identity = 87/200 (43.50%), Postives = 136/200 (68.00%), Query Frame = 0


Query: 2    EAAEDYRIRKKKGLLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIAGCLRNTNFSI 61
            E  ++ R  +++G++ K+D EKAYD V+W FLD +L +KGFG  WR+W+ GCL + +F++
Sbjct: 1413 EIVDEKRRSREEGVVFKIDFEKAYDHVSWDFLDHVLEMKGFGIRWRKWMRGCLSSVSFAV 1472

Query: 62   MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
            ++NG  +G + ASRGLRQGDPLSPFLFTIV D + + +    E+ +L+G+++G N+  +S
Sbjct: 1473 LVNGNAKGWVKASRGLRQGDPLSPFLFTIVADVLSRMLLKAEEKNVLEGFKVGRNRTRVS 1532

Query: 122  MLQYADDTLIFCPNSEADLQGWKEIILLIMEGSGLRLNLLKSSLIGIKVDKDELDLWATK 181
             LQ+ADDT+ F  + E D+   K ++L+    SGL++NL KS++ GI ++++ L   A  
Sbjct: 1533 HLQFADDTIFFSSSREEDMMTLKNVLLVFGHISGLKVNLDKSNIYGINLEQNHLSRLAEM 1592

Query: 182  FGCHSNELPFNYLGFQLGGN 202
              C ++  P  YLG  LGGN
Sbjct: 1593 LDCKASGWPILYLGLPLGGN 1612

[Close]

BLAST of Lcy03g004230 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 0


Query: 62  MINGRPRGKIVASRGLRQGDPLSPFLFTIVGDAVCKFVKFCLERKILKGWEIGVNKVELS 121
           +ING P+G +  SRGLRQGDPLSP+LF +  + +    +   E+  L G  +  N   ++
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 122 MLQYADDT 130
            L +ADDT
Sbjct: 73  HLLFADDT 80

[Close]

BLAST of Lcy03g004230 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 49.3 bits (116), Expect = 4.8e-06
Identity = 23/49 (46.94%), Postives = 33/49 (67.35%), Query Frame = 0


Query: 7  YRIRKKKG----LLLKLDLEKAYDMVNWQFLDEILALKGFGQIWRRWIA 52
          + +R+KKG    +LLKLDLEKAYD + W +L++ L   GF ++W   IA
Sbjct: 38 HSMRRKKGVKGWMLLKLDLEKAYDRIRWDYLEDTLISAGFPEVWLPEIA 86

The following BLAST results are available for this feature:

BLAST of Lcy03g004230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O00370	2.3e-13	28.21	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P11369	1.5e-12	28.21	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
P08548	2.6e-12	28.85	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
P92555	2.3e-08	41.18	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q03274	1.5e-07	29.63	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of Lcy03g004230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E196	4.1e-53	52.66	uncharacterized protein LOC111025019 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A803QQM3	8.0e-49	49.27	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803QEA6	1.0e-48	49.27	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803P8A0	1.8e-48	50.50	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803QI00	6.7e-48	48.50	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lcy03g004230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158566.1	8.4e-53	52.66	uncharacterized protein LOC111025019 [Momordica charantia]	[more]
XP_009335445.1	1.1e-47	48.74	PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri]	[more]
RVX20162.1	1.2e-46	45.50	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]
RVW91328.1	2.6e-46	44.00	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]
RVX14115.1	2.6e-46	43.50	Protein SWEETIE [Vitis vinifera]	[more]

BLAST of Lcy03g004230 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG01250.1	1.6e-09	41.18	RNA-directed DNA polymerase (reverse transcriptase)	[more]
AT4G20520.1	4.8e-06	46.94	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 7..198 e-value: 8.8E-30 score: 103.9
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 1..198 score: 17.87468
None	No IPR available	PANTHER	PTHR33116:SF33	OS01G0885550 PROTEIN	coord: 10..205
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 10..205
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 12..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g004230.1	Lcy03g004230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0044238	primary metabolic process
biological_process	GO:0048583	regulation of response to stimulus
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0016020	membrane
molecular_function	GO:0003824	catalytic activity