Lag0041084 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0041084
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein RALF-like 4
Locationchr13: 11800371 .. 11800724 (-)
RNA-Seq ExpressionLag0041084
SyntenyLag0041084
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCCAATAAAGCTCTGCCTATTCCTCGTCCTGATCGTCGTCGCCACTGCCCCTCGCTCCTCCGCCATCAAGGACCGGTTCCGTGCCTATGGCAACCCCGAATTCGCCTACTTCGGTGTAAATATCGTACCGGCATCGTTGGACAAAGACAGTGGCCGACTGCTTTTCCAATACGGATATAAATACAAGTACCCACCCAGCAAGTACATTAGCTACGAAGCGCTTAAGGCGAACAATATTCCTTGTGGCAAACGTGGAGCCTCTTACTATCATTGCGTCAAGCGAATAAAGGCCAACCCCTATACTCGTGGTTGCAGCTTCAGGACTGGCTGCGCTCGCTACACTGATTAA

mRNA sequence

ATGGGCCCAATAAAGCTCTGCCTATTCCTCGTCCTGATCGTCGTCGCCACTGCCCCTCGCTCCTCCGCCATCAAGGACCGGTTCCGTGCCTATGGCAACCCCGAATTCGCCTACTTCGGTGTAAATATCGTACCGGCATCGTTGGACAAAGACAGTGGCCGACTGCTTTTCCAATACGGATATAAATACAAGTACCCACCCAGCAAGTACATTAGCTACGAAGCGCTTAAGGCGAACAATATTCCTTGTGGCAAACGTGGAGCCTCTTACTATCATTGCGTCAAGCGAATAAAGGCCAACCCCTATACTCGTGGTTGCAGCTTCAGGACTGGCTGCGCTCGCTACACTGATTAA

Coding sequence (CDS)

ATGGGCCCAATAAAGCTCTGCCTATTCCTCGTCCTGATCGTCGTCGCCACTGCCCCTCGCTCCTCCGCCATCAAGGACCGGTTCCGTGCCTATGGCAACCCCGAATTCGCCTACTTCGGTGTAAATATCGTACCGGCATCGTTGGACAAAGACAGTGGCCGACTGCTTTTCCAATACGGATATAAATACAAGTACCCACCCAGCAAGTACATTAGCTACGAAGCGCTTAAGGCGAACAATATTCCTTGTGGCAAACGTGGAGCCTCTTACTATCATTGCGTCAAGCGAATAAAGGCCAACCCCTATACTCGTGGTTGCAGCTTCAGGACTGGCTGCGCTCGCTACACTGATTAA

Protein sequence

MGPIKLCLFLVLIVVATAPRSSAIKDRFRAYGNPEFAYFGVNIVPASLDKDSGRLLFQYGYKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD
Homology
BLAST of Lag0041084 vs. NCBI nr
Match: XP_038876152.1 (uncharacterized protein LOC120068449 [Benincasa hispida])

HSP 1 Score: 140.6 bits (353), Expect = 8.7e-30
Identity = 67/117 (57.26%), Postives = 86/117 (73.50%), Query Frame = 0

Query: 1   MGPIKLCLFLVLIVVATAPRSSAIKDRFRAYGNPEFAYFGVNIVPASLDKDSGRLLFQYG 60
           MG +KLCLFL L+V A +P S A  D   AYG PE+A++G +  P +  +D+ RLLFQYG
Sbjct: 1   MGTMKLCLFL-LLVAAVSPLSFAYSDWTHAYGAPEYAFWG-SSTPTATIEDNRRLLFQYG 60

Query: 61  YKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD 118
           + YKYP ++Y+SYEAL+ NN+PCG RG SYY C +  KANPY RGC+  TGCAR+TD
Sbjct: 61  FAYKYPRNRYLSYEALRKNNVPCGHRGRSYYDCNQHQKANPYRRGCTAITGCARFTD 115

BLAST of Lag0041084 vs. NCBI nr
Match: XP_016902249.1 (PREDICTED: protein RALF-like 4 [Cucumis melo] >KAA0033467.1 protein RALF-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 132.9 bits (333), Expect = 1.8e-27
Identity = 67/117 (57.26%), Postives = 85/117 (72.65%), Query Frame = 0

Query: 4   IKLCLFLVLIVVA-TAPRSSAIKDRFRAYGNPEFAYFGVNIVPASLD--KDSGRLLFQYG 63
           +KLCL L+L+V A  A    A+ D  RA+GNPE+A++G N  PA+++   DS RLLFQYG
Sbjct: 1   MKLCLCLLLLVAAFAATLPVALCDWTRAFGNPEYAFWG-NSAPATINDVDDSRRLLFQYG 60

Query: 64  YKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD 118
           + YKYP +KY+ Y+AL+ NN PC +RG SYY C KR KANPY RGC   TGCAR+TD
Sbjct: 61  FAYKYPKNKYLGYDALRKNNSPCRRRGHSYYDCTKRRKANPYRRGCIAITGCARFTD 116

BLAST of Lag0041084 vs. NCBI nr
Match: XP_004150814.1 (protein RALF-like 4 [Cucumis sativus] >KGN47203.1 hypothetical protein Csa_020789 [Cucumis sativus])

HSP 1 Score: 124.4 bits (311), Expect = 6.5e-25
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = 0

Query: 4   IKLCLFLVLIVVATA-PRSSAIKDRFRAYGNPEFAYFGVNIVPASLD--KDSGRLLFQYG 63
           +KL L L+L+V A A     A+ D  RAYGNP++A++G    PA+++   DS RLLFQYG
Sbjct: 1   MKLGLCLLLLVAAIAITLPVALCDWTRAYGNPDYAFWG-TAEPATINDVDDSRRLLFQYG 60

Query: 64  YKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD 118
           + YKYP +KY+ Y+AL+ NN PC  RG SYY C KR KANPY RGC   TGCAR+TD
Sbjct: 61  FAYKYPKNKYLGYDALRKNNSPCRHRGHSYYDCTKRRKANPYRRGCIAITGCARFTD 116

BLAST of Lag0041084 vs. NCBI nr
Match: XP_004148669.1 (protein RALF-like 19 [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 1.1e-24
Identity = 64/119 (53.78%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 1   MGPIKLCLFLVLIVVATAPRSSAIKDRF-RAYGN-PEFAYFGVNIVPASLDKDSGRLLFQ 60
           M   KLCLFL+LI V  AP   A+ D + R+Y + P++ +   N       +DS RLLFQ
Sbjct: 1   MRSTKLCLFLLLIAVVAAPLCLALSDDWTRSYADVPDYDFTNSN-------EDSRRLLFQ 60

Query: 61  YGYKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD 118
           YG+ YKYP +KY+ Y+AL+ NNIPC  RG SYY C KR KANPY RGC   TGCAR+TD
Sbjct: 61  YGFAYKYPKNKYLGYDALRKNNIPCRHRGRSYYDCKKRKKANPYRRGCIAITGCARFTD 112

BLAST of Lag0041084 vs. NCBI nr
Match: XP_022925244.1 (protein RALF-like 31 [Cucurbita moschata] >KAG6595448.1 Protein RALF-like 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 121.3 bits (303), Expect = 5.5e-24
Identity = 65/119 (54.62%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 1   MGPIKLCLFLVLIVVATAPRSSAIKDRFRAYGNPEFAYFGVNIVPASLDKDSGRLLFQYG 60
           MG +KLCL L+L+VVA AP  S        Y  P++A+FG    PA L +D  RLLFQYG
Sbjct: 1   MGFMKLCLCLLLMVVAAAPPYS--------YAAPDYAFFG-GYAPAQLTEDGRRLLFQYG 60

Query: 61  Y--KYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYTD 118
           Y    K    +Y+SYEAL  NNIPCG+RG+SYY C KR + NPY RGC+  TGCAR+TD
Sbjct: 61  YDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARFTD 110

BLAST of Lag0041084 vs. ExPASy Swiss-Prot
Match: Q9FZA0 (Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-11
Identity = 41/115 (35.65%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 1   MGPIKLCLFLVLIVVATAPRSSAIKDRFRAYGNPEFAYFGVNIVPASLDKDSGRLLFQYG 60
           MG   L +F +LI+   A   +A     ++  N +      + + + +D ++ R     G
Sbjct: 1   MGVKMLLIFGLLILAMVAKSVNATYPLTKSCINGQGCIGEDDELESLMDSETNRRQLARG 60

Query: 61  YKYKYPPSKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARY 116
                   +YI Y+ALK NN+PC +RG SYY C KR + NPY RGCS  T C RY
Sbjct: 61  -------RRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCSAITHCYRY 108

BLAST of Lag0041084 vs. ExPASy Swiss-Prot
Match: Q6NME6 (Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.2e-11
Identity = 30/47 (63.83%), Postives = 34/47 (72.34%), Query Frame = 0

Query: 70  YISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCARYT 117
           YISY AL+ NN+PC +RG SYY C KR +ANPY RGCS  T C R T
Sbjct: 63  YISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHCYRQT 109

BLAST of Lag0041084 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.6e-10
Identity = 28/47 (59.57%), Postives = 36/47 (76.60%), Query Frame = 0

Query: 68  SKYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCAR 115
           +KYISY AL+ N +PC +RGASYY+C +  +ANPY+RGCS  T C R
Sbjct: 70  TKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

BLAST of Lag0041084 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.1e-10
Identity = 27/46 (58.70%), Postives = 36/46 (78.26%), Query Frame = 0

Query: 69  KYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCAR 115
           KYISY A++ N++PC +RGASYY+C +  +ANPY+RGCS  T C R
Sbjct: 74  KYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCRR 119

BLAST of Lag0041084 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.6e-10
Identity = 28/46 (60.87%), Postives = 35/46 (76.09%), Query Frame = 0

Query: 69  KYISYEALKANNIPCGKRGASYYHCVKRIKANPYTRGCSFRTGCAR 115
           +YISY AL+ N IPC +RGASYY+C +  +ANPY+RGCS  T C R
Sbjct: 92  RYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876152.18.7e-3057.26uncharacterized protein LOC120068449 [Benincasa hispida][more]
XP_016902249.11.8e-2757.26PREDICTED: protein RALF-like 4 [Cucumis melo] >KAA0033467.1 protein RALF-like 4 ... [more]
XP_004150814.16.5e-2555.56protein RALF-like 4 [Cucumis sativus] >KGN47203.1 hypothetical protein Csa_02078... [more]
XP_004148669.11.1e-2453.78protein RALF-like 19 [Cucumis sativus][more]
XP_022925244.15.5e-2454.62protein RALF-like 31 [Cucurbita moschata] >KAG6595448.1 Protein RALF-like 4, par... [more]
Match NameE-valueIdentityDescription
Q9FZA01.1e-1135.65Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=1 SV=1[more]
Q6NME64.2e-1163.83Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1[more]
Q8L9P81.6e-1059.57Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1[more]
Q9MA622.1e-1058.70Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1[more]
Q9LUS73.6e-1060.87Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 68..114
e-value: 8.1E-18
score: 65.2
NoneNo IPR availablePANTHERPTHR33136:SF84PROTEIN RALF-LIKE 19coord: 39..116
NoneNo IPR availablePANTHERPTHR33136RAPID ALKALINIZATION FACTOR-LIKEcoord: 39..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0041084.1Lag0041084.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019722 calcium-mediated signaling