Lag0040375 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040375
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	chr13: 4286397 .. 4288229 (-)
RNA-Seq Expression	Lag0040375
Synteny	Lag0040375

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACATTTTGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTGGTGAGCTCGTTGGCGATGACGAGTGCAGGGATTTCCGAGATTAACGATAAGCATTTGCAGTATTCCAAGTTCTGGAATAGTGCTCCGAAAGCTTCGTCTGAGGTATCTAAGCTTTCGAGCAGATATGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGGCAATGAGAGGATTCTTCTGCTTAAATCTGCTCTTACTTTCCATTTCTTCAAGAGGATCTTTGAGGTACGCTCTCTGTTTCTCTTTACGCCTACGATTGAGTTCTGAAATTTCGATCTGATTCTGGAAATAGGTATAGTAGGACTCGGTTTCATCTCTGATGTTCTTGATTAGCTATTCATATCATCGTAACTGTTAGAATCAGTTAGATTGGGGCTATATGAAGAAACTGTTAATCTTATTCTTCGAAATTTTCTCGAGTTCTTACAAAACTATATGAAAATTCGCCAGCGCAGAGAAAATGGTGGAGAGATCTGCATCTCTTCTCTCTTCTCTGTATCTTATCATTCCTCTGGAAGAATGGGAAGATTCTTAACCTATCTAGACTTATTATTATTATTATTATTATTATTATTATCATCGTCGTATGAAGTATGAACTTGATTAATCATCCGCTCATAAAAATCTGAGTTTATTAATTTTTCCTCTTTGACTATTGAATTGTCGTTAAGATATTCTTTGAGTCAACTCTGCGTAGAACAAGTTTTTCAAAATTCTTGATATATAAAGTTGTATGTTTAATAAAGACAAATTAGCACGATTCTGTTTAACAATTATTTTGTTGTTATGTTTTGGGTATTTTAAATTCTGTTTCACTAATCACGAGATAATCTGTACTGCTTATACCTTTCATTCGTGGCACTATTATTTTACAAACATCCACAATAAAAGCCCAAGAGAAAATCAAATTCATCATGTGTCCAAGATTTAATATCCTATGAGTTATATTGATAGCAGGGTCTGTGATTTCTTGCGAGAATAGTCAAGTTGCATGCAAATGATCCTAACACACACGGATGTTTAAAAAAAAGAAAAATCCGTTGTTTTGCAAACAATTTGAAGATTCCTTTCATATTATAGGAACTATTCTTTTTCATTCTTCAACTATAACAGGATGGAGGTTGAACTATGGACTTTGAAACGATAATAGATATCTCATTCACTGAATTATGTTTAGATTGGCAAATTAGTAAAGCTACGTAGATAACAACAAAACAAAAACCTGACATAGATGCATCGTGACAAAATTTCAATTCTTCACAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTACTGGATACAGCAATCAAGATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTATCACTCGGGCTTCCAGAGCCAGCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTTTGATAGGCATCATTGGGAACTTCTACCACCACTTTCTCTTGTCCAGAACAAGAAAAGGAGGAGAAACAGACTACAAAATTCCCACAGGAGGCCTCTTCAGCCTTGTGATCTGCCCTCACTATCTGTTTGAAATAATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTCTGTGCTTCACCATTGGCACCAGTATCTACTTGGCTGGGAGGAGCTATGCCACTAGAAAATGGTACATCTCCAAATTTGAAAATTTCCCCAAGCATGTTAAATCCCTGCTCCCTTTTGTGTTTTAG

mRNA sequence

ATGGACATTTTGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTGGTGAGCTCGTTGGCGATGACGAGTGCAGGGATTTCCGAGATTAACGATAAGCATTTGCAGTATTCCAAGTTCTGGAATAGTGCTCCGAAAGCTTCGTCTGAGGTATCTAAGCTTTCGAGCAGATATGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGGCAATGAGAGGATTCTTCTGCTTAAATCTGCTCTTACTTTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTACTGGATACAGCAATCAAGATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTATCACTCGGGCTTCCAGAGCCAGCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTTTGATAGGCATCATTGGGAACTTCTACCACCACTTTCTCTTGTCCAGAACAAGAAAAGGAGGAGAAACAGACTACAAAATTCCCACAGGAGGCCTCTTCAGCCTTGTGATCTGCCCTCACTATCTGTTTGAAATAATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTCTGTGCTTCACCATTGGCACCAGTATCTACTTGGCTGGGAGGAGCTATGCCACTAGAAAATGGTACATCTCCAAATTTGAAAATTTCCCCAAGCATGTTAAATCCCTGCTCCCTTTTGTGTTTTAG

Coding sequence (CDS)

ATGGACATTTTGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCCACGTTCGTGAACGTGATGACGGTGGTGAGCTCGTTGGCGATGACGAGTGCAGGGATTTCCGAGATTAACGATAAGCATTTGCAGTATTCCAAGTTCTGGAATAGTGCTCCGAAAGCTTCGTCTGAGGTATCTAAGCTTTCGAGCAGATATGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGGCAATGAGAGGATTCTTCTGCTTAAATCTGCTCTTACTTTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTACTGGATACAGCAATCAAGATCTCCCTCAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTATCACTCGGGCTTCCAGAGCCAGCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTTTGATAGGCATCATTGGGAACTTCTACCACCACTTTCTCTTGTCCAGAACAAGAAAAGGAGGAGAAACAGACTACAAAATTCCCACAGGAGGCCTCTTCAGCCTTGTGATCTGCCCTCACTATCTGTTTGAAATAATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCTCTGTGCTTCACCATTGGCACCAGTATCTACTTGGCTGGGAGGAGCTATGCCACTAGAAAATGGTACATCTCCAAATTTGAAAATTTCCCCAAGCATGTTAAATCCCTGCTCCCTTTTGTGTTTTAG

Protein sequence

MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFWNSAPKASSEVSKLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSKMVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFVF

Homology

[Close]

BLAST of Lag0040375 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 458.8 bits (1179), Expect = 3.3e-125
Identity = 233/266 (87.59%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPPPSTFVNVMTVVSS AM  AGISEIN KHLQYSKFW  NS+PKA SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY +ST IMIYTQHLS GLPEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Lag0040375 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 458.0 bits (1177), Expect = 5.6e-125
Identity = 232/266 (87.22%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPPPSTFVNVMTVVSS AM  AGISEIN KHLQYSKFW  NS+PKA SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY +ST IMIYTQHLS G+PEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Lag0040375 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.4 bits (1173), Expect = 1.6e-124
Identity = 232/266 (87.22%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPP STFVNVMTVVSS AM  AGISEIN KHLQYSKFW  NS+PKA SE +
Sbjct: 300 MGILSAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEET 359

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 360 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 419

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY +ST IMIYTQHLS GLPEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 420 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 479

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 480 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 539

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 540 YATRKWYVSKFENFPSHVKALLPFVF 565

[Close]

BLAST of Lag0040375 vs. NCBI nr
Match: XP_023528760.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456.4 bits (1173), Expect = 1.6e-124
Identity = 233/266 (87.59%), Postives = 241/266 (90.60%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPPPSTFVNVMTVVSS AM  AGISEI  KHLQYSKFW  NSAPKA SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY  ST IMIYTQHLS GLPEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Lag0040375 vs. NCBI nr
Match: XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 453.8 bits (1166), Expect = 1.0e-123
Identity = 229/266 (86.09%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           MD+LSAFLFPPPPS FVN+MTVVSS A+  AGISEIN KHLQYSKFW  N+APK SSEVS
Sbjct: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
            LSS+YGMLLAYTPAFLAGAASFW FPS +ERILLLKSALT HFFKRIFEVLFIHKYSSK
Sbjct: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVL TAI+IS SYF+ST IMIYTQHLS GLPEP I+LKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET Y+IP GGLFS VICPHYLFEIIEFFGFAFISQELYPLCF+IGTSIYLAGRS
Sbjct: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWYISKFENFP HVK+LLPFVF
Sbjct: 274 YATRKWYISKFENFPNHVKALLPFVF 299

[Close]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.3e-17
Identity = 44/107 (41.12%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 158 IGIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFI 217
           IG  L+L G++ N +   +L   RK G+T YKIP GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 218 SQELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFVF 265
           S  +    F   T  +L+GR+    +WY+ KFE +PK  K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 6.8e-17
Identity = 43/107 (40.19%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 158 IGIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFI 217
           IG  L+L G++ N +   +L   RK G+T YKIP GGLF  V   +Y  EI+E+ G+A  
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 218 SQELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFVF 265
           S  +    F   T  +L+GR+    +WY+ KFE +PK  K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 44/105 (41.90%), Postives = 63/105 (60.00%), Query Frame = 0


Query: 159 GIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFIS 218
           G AL+L+G++ N +   +L   RK GET YKIP GGLF  V   +Y  E++E+ GFA  S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209

Query: 219 QELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFV 264
             L  + F + T   L  R+    +WY+ KFE++PK  K L+PF+
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 45/105 (42.86%), Postives = 62/105 (59.05%), Query Frame = 0


Query: 159 GIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFIS 218
           G AL+L+G++ N +   +L   RK GET YKIP GGLF  V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 219 QELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFV 264
             L  + F + T   L  R+    +WY  KFE++PK  K L+PFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.4e-16
Identity = 63/200 (31.50%), Postives = 104/200 (52.00%), Query Frame = 0


Query: 80  SFWFFPSG----NERILLLKSALTFHFFKRIFEVLFIHKYSSKMVLDTAIKISLSYFMST 139
           +F+ FPSG    N +  LL S   FH+F R   VL+  + +          +S++ FM+ 
Sbjct: 62  TFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVA-FMAF 121

Query: 140 AIMIYTQHL-SLGLPEPAIDLKN---------IGIALFLIGIIGNFYHHFLLSRTRKGGE 199
              +   +L +  +     D +N          G+ +F++G+  N     +L   +K G+
Sbjct: 122 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 181

Query: 200 TDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRSYATRKWY 259
             YKIP GGLF LV CP+Y  EI+E+FG+A ++       F + T   L  R+ ATR WY
Sbjct: 182 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 241

Query: 260 ISKF-ENFPKHVKSLLPFVF 265
           + KF +++PK  K+++PF++
Sbjct: 242 LEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of Lag0040375 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.6e-125
Identity = 233/266 (87.59%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPPPSTFVNVMTVVSS AM  AGISEIN KHLQYSKFW  NS+PKA SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY +ST IMIYTQHLS GLPEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Lag0040375 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.7e-125
Identity = 232/266 (87.22%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           M ILSAFLFPPPPSTFVNVMTVVSS AM  AGISEIN KHLQYSKFW  NS+PKA SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLLAYTPAFLAGAASFWFFPS NERIL+LK+ALT HFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTAIKIS SY +ST IMIYTQHLS G+PEP IDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK GET YKIP GGLFSLVICPHYLFEIIEFFGFAFISQELYPLCF+ GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFENFP HVK+LLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Lag0040375 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 221/266 (83.08%), Postives = 237/266 (89.10%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSEVS 60
           MDILSAFLFPPPPSTFVNVMTVVSS ++  AG+SEI  KHLQYSKFW  NS  K S++ S
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           KLSS+YGMLL YTPAFLAGAASFW FPSG+ER  LLKSALT HFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVL TAI+IS SYF+STAIMIYTQHLS GLPEP IDLKNIG+ LFL GIIGNFYHH+LLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK  ET YKIP GGLFS VICPHYLFEII +FGFAFISQELYPLCF+IGT+IYLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWYISKFENFP HVK+LLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of Lag0040375 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.1e-118
Identity = 215/266 (80.83%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFWNS--APKASSEVS 60
           MDI SAFLFPPPPSTFVNVMTVVSS AM  +G SE+N KHLQYSKFWN+  A K+SSE S
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           K+SS+YGMLLAYTPAFLAGAASFWFFPS + RILLLKSALTFHFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVLDTA KISLSYF+STAIMIYTQHLS G P P+IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           RTR  GE +YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF  GT++YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWY+SKFE+FP HVK+LLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

[Close]

BLAST of Lag0040375 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.3e-108
Identity = 205/266 (77.07%), Postives = 226/266 (84.96%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFWNS--APKASSEVS 60
           MD+LSAFLFPPPPSTFVNVMTVVS+ A+  AG+SEIN KHLQYSKFWN+  A K S+E S
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSSK 120
           K+SS+YGMLLAYTPAFLAGAASFW FPSG+ER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVL TAI+ISLSYFMSTAIMIYTQHLS GLPEP IDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 RTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAGRS 240
           +TRK G+T YKIP GGLFS V+CPHYLFEII +FGFAFISQELYPLCF+IGT++YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRKWYISKFENFPKHVKSLLPFVF 265
           YATRKWYISKFE FP +VK+LLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 268

[Close]

BLAST of Lag0040375 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 312.0 bits (798), Expect = 4.6e-85
Identity = 153/268 (57.09%), Postives = 205/268 (76.49%), Query Frame = 0


Query: 1   MDILSAFLFPPPPSTFVNVMTVVSSLAMTSAGISEINDKHLQYSKFW--NSAPKASSE-V 60
           M+++++F++PPPPS  +N M+VV   A+ + G SEI   HL+YSKF   +S+P+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  SKLSSRYGMLLAYTPAFLAGAASFWFFPSGNERILLLKSALTFHFFKRIFEVLFIHKYSS 120
             +SSR GMLL YTPAFLA A+SF+  PS + R LLLKSAL  HFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLKNIGIALFLIGIIGNFYHHFLL 180
            M +D+A+ IS SYF STA+M+Y+Q+L+LGL EP+ D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SRTRK-GGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFTIGTSIYLAG 240
           ++ RK  G+ +YKIP GGLF ++ICPHYLFEI+ F+ F  ISQ +Y   F +GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSYATRKWYISKFENFPKHVKSLLPFVF 265
           RSYATR WY+SKF++FPKH+K+L+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of Lag0040375 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-14
Identity = 42/108 (38.89%), Postives = 62/108 (57.41%), Query Frame = 0


Query: 158 IGIALFLIGIIGNFYHHFLLSRTRKGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFI 217
           IG+ +F+ G+  N      L R +K     Y IP GG F LV CP+Y  E IE+ G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 218 SQELYPLCFTIGTSIYLAGRSYATRKWYISKF-ENFPKHVKSLLPFVF 265
           +     + F + T   L  R+ A+ KWYI+KF E +PK  K+++PFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Lag0040375 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 5.2e-12
Identity = 49/157 (31.21%), Postives = 74/157 (47.13%), Query Frame = 0


Query: 100 FHFFKRIFEVLFIHKYSSKMVLDTAIKISLSYFMSTAIMIYTQHLSLGLPEPAIDLK-NI 159
           FH+FKRI E  F+H++S        +  + +Y+ S    I   +++  L  P  DL+  I
Sbjct: 140 FHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKI 199

Query: 160 GIALFLIGIIGNFYHHFLLSRTR-KGGETDYKIPTGGLFSLVICPHYLFEIIEFFGFAFI 219
           G    L+  + NFY H LL   R   G   Y+IP G LF++V C +Y  EI ++ GF   
Sbjct: 200 GFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIA 259

Query: 220 SQELYPLCFTIGTSIYLAGRSYATRKWYISKFENFPK 255
           +Q       TI   ++LA  +     W + K     K
Sbjct: 260 TQ-------TIAGYVFLAVAALIMTNWALGKHSRLRK 288

[Close]

BLAST of Lag0040375 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 39/115 (33.91%), Postives = 57/115 (49.57%), Query Frame = 0


Query: 155 LKNIGIALFLIGIIGNFYHHFLLSRTRK--GGETDYKIPTGGLFSLVICPHYLFEIIEFF 214
           L+ IG A+FL G I     H +L   R+      +Y IP G  F +V CPH+L EI+ + 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 215 GFAFISQELYPLCFTIGTSIYLAGRSYA---TRKWYISKFENFPKHVKSLLPFVF 265
           G   IS     +   +      A  +YA   T +WY+ KFEN+P    ++ P V+
Sbjct: 267 GL-LISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of Lag0040375 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 55.8 bits (133), Expect = 5.9e-08
Identity = 39/117 (33.33%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 155 LKNIGIALFLIGIIGNFYHHFLLSRTRKGGE--TDYKIPTGGLFSLVICPHYLFEIIEFF 214
           L+ IG A+FL G I     H +L   R+      +Y IP G  F +V  PH+L EI+ + 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 215 GFAFISQ----ELYPLC-FTIGTSIYLAGRSYATRKWYISKFENFPKHVKSLLPFVF 265
           G    S      ++ L  F      Y AG    T +WY+ KFEN+P +  ++ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:

BLAST of Lag0040375 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022957108.1	3.3e-125	87.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_022984951.1	5.6e-125	87.22	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]
KAG7032032.1	1.6e-124	87.22	3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023528760.1	1.6e-124	87.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038891781.1	1.0e-123	86.09	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]

BLAST of Lag0040375 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	2.3e-17	41.12	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	6.8e-17	40.19	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q68FF9	1.5e-16	41.90	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
P24008	1.5e-16	42.86	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q2QDF6	3.4e-16	31.50	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]

BLAST of Lag0040375 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZA3	1.6e-125	87.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J6R3	2.7e-125	87.22	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A1S3BFS0	1.3e-119	83.08	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1DAI6	7.1e-118	80.83	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A0A0KTC7	1.3e-108	77.07	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]

BLAST of Lag0040375 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	4.6e-85	57.09	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	7.2e-14	38.89	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	5.2e-12	31.21	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	1.2e-08	33.91	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	5.9e-08	33.33	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 156..264 e-value: 1.5E-28 score: 99.7
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 159..239 score: 25.431198
None	No IPR available	GENE3D	1.20.120.1630		coord: 89..263 e-value: 1.4E-7 score: 33.4
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 6..264
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 6..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040375.1	Lag0040375.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors