Lag0040161 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040161
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	t-SNARE coiled-coil homology domain-containing protein
Location	chr13: 2591136 .. 2592830 (-)
RNA-Seq Expression	Lag0040161
Synteny	Lag0040161

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTAGCTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGGATTCTGTAGATCCTGGATTATCAGCGGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACCTGAAACCAACCAAAATCTTACTCCTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAAATCCAATTCAGCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTTCAGCCTCTAAAGACAGGTACAAAAATGATTTCCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCTGTGTATAAAGCTGAAGAGACCACAAAATCTGTTAACAACTGCCTGAAGATTGCCGAGGACATAAGGGAAGATGCTACCAGGACCCTTGACATGTTGCATCAACAGGGTGAGCAAATTGAGAGGACTCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGTAGGCTCTTTACTAGTTTCCATCAGGCACCTTTCGACTTTATTTTCTATCTTATGCTGTTATCCTGAAATGATAAGTTGGCCCATGTTGATCTGATATATTTAAAAAGAGAATCAGATTATTCAAAATTTGAAAGATAGCTGTTACGAAATATTTGGCTTGCAAGCTTTTATGGTTAGGTTGTGGATATCTACTCTGTAGGAGAGGGAAAATGATGGAGAACGAAGGTGAAAGTGAGCTGTTTTGAAGTATTTGGCTTGCAAGTTTTTGTGATTACCTAGGTTGTGGATGTCCTACTCTGTAAGAGATGGAAAATGATCAAGAACGAATAACCTTTTAAGTTTTCAAGGAATCTGATATGCTAGCCAGAAAATGAATGAAGTTATTTTTGTCTTGCTTTTCCTTTGTTTATTGTATGTGTTCTATGTTCTCCGCATTTGTTAATAGTTTCCTATTCATTATTGTTAATTAATAGGGAGAGAAGCTTCTTAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGACATTACAGGCCCTCTTATAACAGCAGGTTATATTTTTTCACTGGTTCTTTTGCTCACTAAATACTGCCATTCTTGCAACTTCCACTCCCATTTTATCTTCTATTGATTATGTCCATTTTGGCTGTCCAGATAATCCTTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGTATCTACGGGCAAAAAACAGTCAGCTAAAACACCACCTTCTGAACCATCAGGTGCAATACAAAAAGTTGAGGTGAGAGCATTGGCTGTTATCATTTCACTATCTTCAGAGAGTATAGCATTATATGGTTTCAGATTTAACCAATAGGTAAAAATGTTGACACAGGTTGAGAAGGAAAAGCAAGATGATGCACTTTCCGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGATAGGTCATATAAGCCATCCATCCTCTTTTCTCAATTTAGAGATTCTTGTATTATTATCTCATCAATCTGTTGCCTTATTACATAGTTCTGAATGCATATTTTCTGGAAAATGCAGGCAAAACAAAGCTCTGGATCATCTAAGTGATGATGTCGATGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGCTCGATAAGTAA

mRNA sequence

ATGTTTAGCTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGGATTCTGTAGATCCTGGATTATCAGCGGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACCTGAAACCAACCAAAATCTTACTCCTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAAATCCAATTCAGCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTTCAGCCTCTAAAGACAGGTACAAAAATGATTTCCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCTGTGTATAAAGCTGAAGAGACCACAAAATCTGTTAACAACTGCCTGAAGATTGCCGAGGACATAAGGGAAGATGCTACCAGGACCCTTGACATGTTGCATCAACAGGGTGAGCAAATTGAGAGGACTCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGGAGAGAAGCTTCTTAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGACATTACAGGCCCTCTTATAACAGCAGATAATCCTTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGTATCTACGGGCAAAAAACAGTCAGCTAAAACACCACCTTCTGAACCATCAGGTGCAATACAAAAAGTTGAGGTTGAGAAGGAAAAGCAAGATGATGCACTTTCCGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGATAGGCAAAACAAAGCTCTGGATCATCTAAGTGATGATGTCGATGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGCTCGATAAGTAA

Coding sequence (CDS)

ATGTTTAGCTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGGATTCTGTAGATCCTGGATTATCAGCGGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACCTGAAACCAACCAAAATCTTACTCCTGCAAGAAGAACTTCTTCCGAGCCTGTGCTCGTTATACCGAAATCCAATTCAGCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTTCAGCCTCTAAAGACAGGTACAAAAATGATTTCCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCTGTGTATAAAGCTGAAGAGACCACAAAATCTGTTAACAACTGCCTGAAGATTGCCGAGGACATAAGGGAAGATGCTACCAGGACCCTTGACATGTTGCATCAACAGGGTGAGCAAATTGAGAGGACTCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGGAGAGAAGCTTCTTAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGACATTACAGGCCCTCTTATAACAGCAGATAATCCTTCTGGGAGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTGGGTGTATCTACGGGCAAAAAACAGTCAGCTAAAACACCACCTTCTGAACCATCAGGTGCAATACAAAAAGTTGAGGTTGAGAAGGAAAAGCAAGATGATGCACTTTCCGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGATAGGCAAAACAAAGCTCTGGATCATCTAAGTGATGATGTCGATGAGCTGAACTCTAGAGTGAAAGGCGCCAATCAACGAGCTCGCCGTTTGCTCGATAAGTAA

Protein sequence

MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSNSANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKTPPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARRLLDK

Homology

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BLAST of Lag0040161 vs. NCBI nr
Match: XP_038891847.1 (putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 putative SNAP25 homologous protein SNAP30 [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 3.8e-146
Identity = 276/304 (90.79%), Postives = 289/304 (95.07%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MF FMKSPAKVTKQ+SVDP LS GSGTNPFDSDTGP+  Q L  ARRTSSEPVL++P S 
Sbjct: 1   MFGFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLIMPNSM 60

Query: 61  SANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
           S+NPFDDDD+GFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS
Sbjct: 61  SSNPFDDDDEGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVEV 240
           WKPKKTR+ITGPLITADN SGRTENNKEQREKLGVSTGKKQSA +TPPSE SGA+QKVE 
Sbjct: 181 WKPKKTREITGPLITADNSSGRTENNKEQREKLGVSTGKKQSATQTPPSEQSGAMQKVEA 240

Query: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR+
Sbjct: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARK 300

Query: 301 LLDK 304
           L+ K
Sbjct: 301 LIGK 304

[Close]

BLAST of Lag0040161 vs. NCBI nr
Match: XP_004135151.1 (putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_031740914.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >KGN51968.1 hypothetical protein Csa_009347 [Cucumis sativus])

HSP 1 Score: 519.2 bits (1336), Expect = 2.3e-143
Identity = 276/305 (90.49%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVDP LS GSGTNPFDSDTGP+  Q L  ARRTSSEPVL +PK  
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPK-- 60

Query: 61  SANPFDDDDD-GFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
            ANPFDDDDD GFVGR+GTATSS SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  -ANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SG+TENNKEQREKLG+STGKKQSA KTPPSEPSGAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
           VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLIGK 302

[Close]

BLAST of Lag0040161 vs. NCBI nr
Match: XP_022984084.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 514.2 bits (1323), Expect = 7.5e-142
Identity = 275/305 (90.16%), Postives = 287/305 (94.10%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVD  LS GSGTNPFDSD+GPE  Q L  ARRTSSEPVLVIP S 
Sbjct: 1   MFSFMKSPAKVTKQNSVDE-LSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SANPF-DDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
           SAN F DDDDDGFVG+RGTATS+ASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SGRTENNKEQREKLGVSTGKKQSA KTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLVGK 304

[Close]

BLAST of Lag0040161 vs. NCBI nr
Match: XP_008446422.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo])

HSP 1 Score: 514.2 bits (1323), Expect = 7.5e-142
Identity = 273/304 (89.80%), Postives = 287/304 (94.41%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVDP LS GSGTNPFDSDTGP+  Q L  ARRTSSEPVL IP   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIP--- 60

Query: 61  SANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
           +ANPF DDDDGFVGR+GTATSS SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS
Sbjct: 61  NANPF-DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVEV 240
           WKPKKT++ITGPLITAD+ SG+TENNK+QREKLG+STGKKQSA KTPPSEPSGA+QKVEV
Sbjct: 181 WKPKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEV 240

Query: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 
Sbjct: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LLDK 304
           L+ K
Sbjct: 301 LIGK 300

[Close]

BLAST of Lag0040161 vs. NCBI nr
Match: XP_022957363.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 putative SNAP25-likeous protein SNAP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.5 bits (1303), Expect = 1.6e-139
Identity = 271/305 (88.85%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVD  LS GSGTNPFDSD+GPE  Q L  ARRTSSEPVL+IP S 
Sbjct: 1   MFSFMKSPAKVTKQNSVDE-LSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSI 60

Query: 61  SANPF-DDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
           SAN F DDDDDGFVG+R TATS+ASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQS-AKTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SGRTENNKEQREKLGVSTGKKQS  KTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLVGK 304

[Close]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-91
Identity = 185/303 (61.06%), Postives = 216/303 (71.29%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MF F KSP                 G N   +++       +T  RRTSSEP+L+ P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  SANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
               FDDD                 D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK 
Sbjct: 61  ---DFDDD-----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKTPPSEPSGAIQKVEVE 240
           WKPKKT++ITGP+IT D PS ++EN+KE+REKLG+    + S++    +P+ A+QKVE E
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLGAKGRSSSQPALDQPTNALQKVEQE 240

Query: 241 KEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARRL 300
           K KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR L
Sbjct: 241 KAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHL 263

Query: 301 LDK 304
           L K
Sbjct: 301 LSK 263

[Close]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.5e-84
Identity = 180/307 (58.63%), Postives = 220/307 (71.66%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MF   KSPA + K +SVD      S  NPFDSD   +    L P++RT+SEP L    ++
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SANPFDDD--DDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETT 120
             NPF  +    G       +  S SK +YKN+FRDSGG+ENQSVQELE YAVYKAEETT
Sbjct: 61  MTNPFGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETT 120

Query: 121 KSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFS 180
           KSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFS
Sbjct: 121 KSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFS 180

Query: 181 KPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKT--PPSEPSGAIQK 240
           K WKPKKTR I GP++T D+   R  N+ E+REKLG+++  +  ++T  P  E + A Q+
Sbjct: 181 KTWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQR 240

Query: 241 VEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 300
           VE+EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL DDVDELN RV+ +NQR
Sbjct: 241 VEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQR 300

Query: 301 ARRLLDK 304
            RRLL K
Sbjct: 301 GRRLLGK 300

[Close]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-59
Identity = 132/246 (53.66%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 58  KSNSANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEET 117
           KS S NPFDDDDD              + R+ +  + SGG ENQ+VQELE+YAVY +EET
Sbjct: 19  KSFSLNPFDDDDD----------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEET 78

Query: 118 TKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 79  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 138

Query: 178 SKPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKTPPSEPSGAIQKV 237
           S+ WKPKK+R ITGP+IT  +   R   + + REKLG++   K  +KT P E   A QK 
Sbjct: 139 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP-EAVDAYQKT 198

Query: 238 EVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRA 297
           ++   KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQRA
Sbjct: 199 QI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRA 251

Query: 298 RRLLDK 304
           R LL K
Sbjct: 259 RYLLRK 251

[Close]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot
Match: P36977 (Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-15
Identity = 61/208 (29.33%), Postives = 109/208 (52.40%), Query Frame = 0


Query: 103 VQELENYAVYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKD 162
           + +++  A   A+E+ +S    L++ E+ ++   RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LSDMQQRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTRDITGPLITADNPSGRTENNK----EQREKLGV 222
           +   EK LN+LG   G+ S P    K+    G     +N  G   +      ++RE++ +
Sbjct: 71  MKDAEKNLNDLGKFCGLCSCPCNKMKS---GGSKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 223 STGKKQSAKTPPSEPSGAIQKV--EVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQN 282
           S               G I++V  +  + + D+ L  +  I+G+L+ MA+DMG+E+D QN
Sbjct: 131 S--------------GGFIRRVTDDARENEMDENLEQVGGIIGNLRHMALDMGNEIDTQN 190

Query: 283 KALDHLSDDVDELNSRVKGANQRARRLL 302
           + +D + +  D   +R+  ANQRA ++L
Sbjct: 191 RQIDRIMEKADSNKTRIDEANQRATKML 201

[Close]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-15
Identity = 61/208 (29.33%), Postives = 110/208 (52.88%), Query Frame = 0


Query: 103 VQELENYAVYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKD 162
           ++E++  A   A+E+ +S    L++ E+ ++   RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTRDITGPLITADNPSGRTENNK----EQREKLGV 222
           + + EK L +LG   G+   P    K+ D        +N  G   +      ++RE++ +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSD-AYKKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 223 STGKKQSAKTPPSEPSGAIQKV--EVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQN 282
           S               G I++V  +  + + D+ L  +S I+G+L+ MA+DMG+E+D QN
Sbjct: 131 S--------------GGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQN 190

Query: 283 KALDHLSDDVDELNSRVKGANQRARRLL 302
           + +D + +  D   +R+  ANQRA ++L
Sbjct: 191 RQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of Lag0040161 vs. ExPASy TrEMBL
Match: A0A0A0KQT5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606590 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 276/305 (90.49%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVDP LS GSGTNPFDSDTGP+  Q L  ARRTSSEPVL +PK  
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPK-- 60

Query: 61  SANPFDDDDD-GFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
            ANPFDDDDD GFVGR+GTATSS SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  -ANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SG+TENNKEQREKLG+STGKKQSA KTPPSEPSGAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
           VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLIGK 302

[Close]

BLAST of Lag0040161 vs. ExPASy TrEMBL
Match: A0A6J1J9F6 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111482502 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.6e-142
Identity = 275/305 (90.16%), Postives = 287/305 (94.10%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVD  LS GSGTNPFDSD+GPE  Q L  ARRTSSEPVLVIP S 
Sbjct: 1   MFSFMKSPAKVTKQNSVDE-LSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SANPF-DDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
           SAN F DDDDDGFVG+RGTATS+ASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SGRTENNKEQREKLGVSTGKKQSA KTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLVGK 304

[Close]

BLAST of Lag0040161 vs. ExPASy TrEMBL
Match: A0A1S3BF13 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103489173 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.6e-142
Identity = 273/304 (89.80%), Postives = 287/304 (94.41%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVDP LS GSGTNPFDSDTGP+  Q L  ARRTSSEPVL IP   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIP--- 60

Query: 61  SANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
           +ANPF DDDDGFVGR+GTATSS SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS
Sbjct: 61  NANPF-DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KTPPSEPSGAIQKVEV 240
           WKPKKT++ITGPLITAD+ SG+TENNK+QREKLG+STGKKQSA KTPPSEPSGA+QKVEV
Sbjct: 181 WKPKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEV 240

Query: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 
Sbjct: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LLDK 304
           L+ K
Sbjct: 301 LIGK 300

[Close]

BLAST of Lag0040161 vs. ExPASy TrEMBL
Match: A0A6J1H000 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111458786 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 7.6e-140
Identity = 271/305 (88.85%), Postives = 285/305 (93.44%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSPAKVTKQ+SVD  LS GSGTNPFDSD+GPE  Q L  ARRTSSEPVL+IP S 
Sbjct: 1   MFSFMKSPAKVTKQNSVDE-LSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSI 60

Query: 61  SANPF-DDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
           SAN F DDDDDGFVG+R TATS+ASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  SANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV+NCLKIAEDIREDAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQS-AKTPPSEPSGAIQKVE 240
           PWKPKKT++ITGPLITAD+ SGRTENNKEQREKLGVSTGKKQS  KTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLLDK 304
            L+ K
Sbjct: 301 HLVGK 304

[Close]

BLAST of Lag0040161 vs. ExPASy TrEMBL
Match: A0A6J1G0W1 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111449670 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.9e-139
Identity = 275/316 (87.03%), Postives = 285/316 (90.19%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MFSFMKSP KV+KQ+SVD GL AGSGTNPFDSDT PETNQ L PARRTSSEP LVIP S 
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGL-AGSGTNPFDSDTEPETNQTLNPARRTSSEPALVIPNSI 60

Query: 61  SANPF--DDDDDGFVGRRGT------------ATSSASKDRYKNDFRDSGGLENQSVQEL 120
           SANPF  DDDDDGFVGRRGT            ATSSASK+ YKNDFRDSGG ENQSVQEL
Sbjct: 61  SANPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQEL 120

Query: 121 ENYAVYKAEETTKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSA-KT 240
           EKLLNNLGGMFSKPWKPKKT++ITGPLITADN SGRTENNKE+REKLG+STGKKQSA +T
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKQSATRT 240

Query: 241 PPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDE 300
           PPSE SGA+QKVE EKEKQDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHLSDDVDE
Sbjct: 241 PPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300

Query: 301 LNSRVKGANQRARRLL 302
           LNSRVKGANQRARRLL
Sbjct: 301 LNSRVKGANQRARRLL 315

[Close]

BLAST of Lag0040161 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 337.8 bits (865), Expect = 9.0e-93
Identity = 185/303 (61.06%), Postives = 216/303 (71.29%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MF F KSP                 G N   +++       +T  RRTSSEP+L+ P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  SANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
               FDDD                 D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK 
Sbjct: 61  ---DFDDD-----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKTPPSEPSGAIQKVEVE 240
           WKPKKT++ITGP+IT D PS ++EN+KE+REKLG+    + S++    +P+ A+QKVE E
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLGAKGRSSSQPALDQPTNALQKVEQE 240

Query: 241 KEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARRL 300
           K KQDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR L
Sbjct: 241 KAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHL 263

Query: 301 LDK 304
           L K
Sbjct: 301 LSK 263

[Close]

BLAST of Lag0040161 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 314.3 bits (804), Expect = 1.1e-85
Identity = 180/307 (58.63%), Postives = 220/307 (71.66%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQDSVDPGLSAGSGTNPFDSDTGPETNQNLTPARRTSSEPVLVIPKSN 60
           MF   KSPA + K +SVD      S  NPFDSD   +    L P++RT+SEP L    ++
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SANPFDDD--DDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETT 120
             NPF  +    G       +  S SK +YKN+FRDSGG+ENQSVQELE YAVYKAEETT
Sbjct: 61  MTNPFGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETT 120

Query: 121 KSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFS 180
           KSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFS
Sbjct: 121 KSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFS 180

Query: 181 KPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKT--PPSEPSGAIQK 240
           K WKPKKTR I GP++T D+   R  N+ E+REKLG+++  +  ++T  P  E + A Q+
Sbjct: 181 KTWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQR 240

Query: 241 VEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 300
           VE+EK KQDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL DDVDELN RV+ +NQR
Sbjct: 241 VEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQR 300

Query: 301 ARRLLDK 304
            RRLL K
Sbjct: 301 GRRLLGK 300

[Close]

BLAST of Lag0040161 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 231.5 bits (589), Expect = 9.0e-61
Identity = 132/246 (53.66%), Postives = 173/246 (70.33%), Query Frame = 0


Query: 58  KSNSANPFDDDDDGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEET 117
           KS S NPFDDDDD              + R+ +  + SGG ENQ+VQELE+YAVY +EET
Sbjct: 19  KSFSLNPFDDDDD----------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEET 78

Query: 118 TKSVNNCLKIAEDIREDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 79  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 138

Query: 178 SKPWKPKKTRDITGPLITADNPSGRTENNKEQREKLGVSTGKKQSAKTPPSEPSGAIQKV 237
           S+ WKPKK+R ITGP+IT  +   R   + + REKLG++   K  +KT P E   A QK 
Sbjct: 139 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP-EAVDAYQKT 198

Query: 238 EVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRA 297
           ++   KQD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQRA
Sbjct: 199 QI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRA 251

Query: 298 RRLLDK 304
           R LL K
Sbjct: 259 RYLLRK 251

[Close]

BLAST of Lag0040161 vs. TAIR 10
Match: AT3G45280.1 (syntaxin of plants 72 )

HSP 1 Score: 43.1 bits (100), Expect = 4.6e-04
Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 229 EPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNS 288
           E S   Q+ E+ ++KQD+ L  +S  L  LK++A DM  ELD+Q   ++ +   VD   S
Sbjct: 164 ESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKVDGATS 223

Query: 289 RVKGANQRARRLL 302
            +K  N R ++ L
Sbjct: 224 DLKNTNVRLKKQL 236

[Close]

BLAST of Lag0040161 vs. TAIR 10
Match: AT3G61450.2 (syntaxin of plants 73 )

HSP 1 Score: 42.4 bits (98), Expect = 7.8e-04
Identity = 25/78 (32.05%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 226 PPSEPSGAIQKVEVEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDE 285
           P  E     Q+ E+++ KQD  L  ++  L  LK+MA D+  ELDRQ   +D +   +D+
Sbjct: 158 PTGESDQFKQEYEMKRIKQDQGLDYIAEGLDTLKNMAQDINEELDRQEPLMDEIDTKIDK 217

Query: 286 LNSRVKGANQRARRLLDK 304
             + +K  N R +  + K
Sbjct: 218 AATDLKSTNVRLKDTVTK 235

The following BLAST results are available for this feature:

BLAST of Lag0040161 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891847.1	3.8e-146	90.79	putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 pu...	[more]
XP_004135151.1	2.3e-143	90.49	putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 puta...	[more]
XP_022984084.1	7.5e-142	90.16	putative SNAP25 homologous protein SNAP30 [Cucurbita maxima]	[more]
XP_008446422.1	7.5e-142	89.80	PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo]	[more]
XP_022957363.1	1.6e-139	88.85	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 put...	[more]

BLAST of Lag0040161 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMG8	1.3e-91	61.06	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9S7P9	1.5e-84	58.63	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9SD96	1.3e-59	53.66	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
P36977	1.1e-15	29.33	Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=...	[more]
Q17QQ3	1.9e-15	29.33	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]

BLAST of Lag0040161 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQT5	1.1e-143	90.49	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A6J1J9F6	3.6e-142	90.16	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A1S3BF13	3.6e-142	89.80	putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10348917...	[more]
A0A6J1H000	7.6e-140	88.85	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1G0W1	2.9e-139	87.03	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Lag0040161 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13890.1	9.0e-93	61.06	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G61210.1	1.1e-85	58.63	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT5G07880.1	9.0e-61	53.66	synaptosomal-associated protein SNAP25-like 29	[more]
AT3G45280.1	4.6e-04	35.62	syntaxin of plants 72	[more]
AT3G61450.2	7.8e-04	32.05	syntaxin of plants 73	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 125..145
None	No IPR available	COILS	Coil	Coil	coord: 269..303
None	No IPR available	GENE3D	1.20.5.110		coord: 97..175 e-value: 3.6E-22 score: 80.1
None	No IPR available	GENE3D	1.20.5.110		coord: 220..302 e-value: 3.5E-23 score: 83.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 203..217
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..245
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 18..57
None	No IPR available	PANTHER	PTHR19305:SF25	SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATED	coord: 1..303
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 1..303
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 241..299 e-value: 1.01924E-16 score: 71.0504
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 234..301
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 101..172
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 233..300 e-value: 4.9E-11 score: 52.7 coord: 106..173 e-value: 0.11 score: 21.7
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 238..300 score: 17.540749
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 108..172 e-value: 5.42935E-22 score: 85.3175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040161.1	Lag0040161.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity