Lag0040063 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040063
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Thioredoxin h
Location	chr13: 1899337 .. 1899920 (-)
RNA-Seq Expression	Lag0040063
Synteny	Lag0040063

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCAAGCTTTACTGTCCCACGTTCAAAATCTTCCAAATCAACAATACAAGAAAAGGCTCCCACCATTGTCGAGTGCCGCAACAAAGCTCAGTGGACAGCTCGTTTCGAAGCCACAAAGGAAACAAACAAGCTTGTAAGTTTTCATATGAACTTTCAATCCCCAACATATGGTAATGCATGCTTCGGCTTTGTCGAGCTTATTGTACTTGGTGATTTGTTGTGCTGCTGGTTTGGTATAGATGGTGATTGATTTCACTGCTACTTGGTGTGGGCCTTGTCGACACATGGAGCCAACCATCAAGGAGTTTGCTGCAAGGTTCAAGGACGTCGAGTTCATCAAAATCGACGTCGATGAGTTGACGGTAGGAGCTTTCAATCTTGTTTTGTCTGTGTTTTGTTTTTTGGTTCAGTGGATGAGTTGAAACGTTACCGTGTTGACAACAGGATGTTGCAAAGGAATATGGCGTGGAGGCAATGCCAACATTCATACTGATCAAGAACGGGAAAGTAATTGATAAGGTGGTCGGAGCCAGGAGGGAAGACCTGCAGAAGAAGATTGAGAAACATAGTAAATATTGA

mRNA sequence

ATGGGAGCAAGCTTTACTGTCCCACGTTCAAAATCTTCCAAATCAACAATACAAGAAAAGGCTCCCACCATTGTCGAGTGCCGCAACAAAGCTCAGTGGACAGCTCGTTTCGAAGCCACAAAGGAAACAAACAAGCTTATGGTGATTGATTTCACTGCTACTTGGTGTGGGCCTTGTCGACACATGGAGCCAACCATCAAGGAGTTTGCTGCAAGGTTCAAGGACGTCGAGTTCATCAAAATCGACGTCGATGAGTTGACGGATGTTGCAAAGGAATATGGCGTGGAGGCAATGCCAACATTCATACTGATCAAGAACGGGAAAGTAATTGATAAGGTGGTCGGAGCCAGGAGGGAAGACCTGCAGAAGAAGATTGAGAAACATAGTAAATATTGA

Coding sequence (CDS)

ATGGGAGCAAGCTTTACTGTCCCACGTTCAAAATCTTCCAAATCAACAATACAAGAAAAGGCTCCCACCATTGTCGAGTGCCGCAACAAAGCTCAGTGGACAGCTCGTTTCGAAGCCACAAAGGAAACAAACAAGCTTATGGTGATTGATTTCACTGCTACTTGGTGTGGGCCTTGTCGACACATGGAGCCAACCATCAAGGAGTTTGCTGCAAGGTTCAAGGACGTCGAGTTCATCAAAATCGACGTCGATGAGTTGACGGATGTTGCAAAGGAATATGGCGTGGAGGCAATGCCAACATTCATACTGATCAAGAACGGGAAAGTAATTGATAAGGTGGTCGGAGCCAGGAGGGAAGACCTGCAGAAGAAGATTGAGAAACATAGTAAATATTGA

Protein sequence

MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEKHSKY

Homology

[Close]

BLAST of Lag0040063 vs. NCBI nr
Match: XP_022151175.1 (thioredoxin H4-like [Momordica charantia])

HSP 1 Score: 244.2 bits (622), Expect = 6.3e-61
Identity = 118/131 (90.08%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCR 60
           MGASFTVPRSKSS +T+Q KAPTIVEC  K QWTARFEA+KETNKL+VIDFTATWCGPCR
Sbjct: 22  MGASFTVPRSKSSNATLQAKAPTIVECHTKPQWTARFEASKETNKLLVIDFTATWCGPCR 81

Query: 61  HMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARRED 120
           HMEPTIKEFAARF DVEF+KIDVDELTDVA+EYGVEAMPTFILIKNGKVIDKVVGAR+ED
Sbjct: 82  HMEPTIKEFAARFVDVEFVKIDVDELTDVAREYGVEAMPTFILIKNGKVIDKVVGARKED 141

Query: 121 LQKKIEKHSKY 132
           LQKKIEKHSKY
Sbjct: 142 LQKKIEKHSKY 152

[Close]

BLAST of Lag0040063 vs. NCBI nr
Match: XP_004135204.1 (thioredoxin H-type [Cucumis sativus] >KGN51870.1 hypothetical protein Csa_007853 [Cucumis sativus])

HSP 1 Score: 243.8 bits (621), Expect = 8.2e-61
Identity = 118/131 (90.08%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCR 60
           MGASFTVPRSKSS + IQ+K PTI+EC +KAQWTARFEATKETNKLMVIDFTA WCGPCR
Sbjct: 1   MGASFTVPRSKSSNAMIQQKPPTIIECHDKAQWTARFEATKETNKLMVIDFTAAWCGPCR 60

Query: 61  HMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARRED 120
           HMEPTIKE AARFKDVEF+KIDVDEL DVAKEYGVEAMPTFILIKNGKVIDKVVGARR+D
Sbjct: 61  HMEPTIKELAARFKDVEFVKIDVDELMDVAKEYGVEAMPTFILIKNGKVIDKVVGARRDD 120

Query: 121 LQKKIEKHSKY 132
           LQKKIEKHSKY
Sbjct: 121 LQKKIEKHSKY 131

[Close]

BLAST of Lag0040063 vs. NCBI nr
Match: XP_038892719.1 (thioredoxin H4-like [Benincasa hispida])

HSP 1 Score: 243.4 bits (620), Expect = 1.1e-60
Identity = 118/131 (90.08%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCR 60
           MGASFTVPRSKSS +T+Q K P IVECRNKAQWTARFEATKETNKLMVIDFTA WCGPCR
Sbjct: 1   MGASFTVPRSKSSNATLQPKTPIIVECRNKAQWTARFEATKETNKLMVIDFTAAWCGPCR 60

Query: 61  HMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARRED 120
           HMEP IKEFAARFKDVEF+KIDVDEL DVA+EYGVEAMPTFILIKNGKV+DKVVGARRED
Sbjct: 61  HMEPIIKEFAARFKDVEFVKIDVDELMDVAQEYGVEAMPTFILIKNGKVVDKVVGARRED 120

Query: 121 LQKKIEKHSKY 132
           LQKKIEK+SKY
Sbjct: 121 LQKKIEKYSKY 131

[Close]

BLAST of Lag0040063 vs. NCBI nr
Match: KAG6574117.1 (Thioredoxin H2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013177.1 Thioredoxin H2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.7 bits (605), Expect = 5.9e-59
Identity = 114/131 (87.02%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCR 60
           MGASFTVPRS+SS ++++ KAPTIVEC NKA+WTA +EATKETNKLMVIDFTA WC PCR
Sbjct: 1   MGASFTVPRSRSSSTSLKGKAPTIVECHNKAEWTAHYEATKETNKLMVIDFTAAWCAPCR 60

Query: 61  HMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARRED 120
           HMEPTIKEFAARFKDVEFIKIDVD LTDVAKEYGVEAMPTFIL+KNGKV+DKVVGARRED
Sbjct: 61  HMEPTIKEFAARFKDVEFIKIDVDTLTDVAKEYGVEAMPTFILLKNGKVVDKVVGARRED 120

Query: 121 LQKKIEKHSKY 132
           LQKKIEK+SKY
Sbjct: 121 LQKKIEKYSKY 131

[Close]

BLAST of Lag0040063 vs. NCBI nr
Match: XP_022945457.1 (thioredoxin H4-like [Cucurbita moschata])

HSP 1 Score: 236.9 bits (603), Expect = 1.0e-58
Identity = 113/131 (86.26%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MGASFTVPRSKSSKSTIQEKAPTIVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCR 60
           MGASFT PRS+SS ++++ KAPTIVEC NKA+WTA +EATKETNKLMVIDFTA WC PCR
Sbjct: 1   MGASFTAPRSRSSSTSLKGKAPTIVECHNKAEWTAHYEATKETNKLMVIDFTAAWCAPCR 60

Query: 61  HMEPTIKEFAARFKDVEFIKIDVDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARRED 120
           HMEPTIKEFAARFKDVEFIKIDVD+LTDVAKEYGVEAMPTFIL+KNGKV+DKVVGARRED
Sbjct: 61  HMEPTIKEFAARFKDVEFIKIDVDKLTDVAKEYGVEAMPTFILLKNGKVVDKVVGARRED 120

Query: 121 LQKKIEKHSKY 132
           LQKKIEK+SKY
Sbjct: 121 LQKKIEKYSKY 131

[Close]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot
Match: Q38879 (Thioredoxin H2 OS=Arabidopsis thaliana OX=3702 GN=TRX2 PE=1 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 2.7e-30
Identity = 54/104 (51.92%), Postives = 82/104 (78.85%), Query Frame = 0


Query: 24  IVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARFKDVEFIKIDV 83
           +++  + A+W   F   KE+NKL+V+DF+A+WCGPCR +EP I   A +F DV+F+K+DV
Sbjct: 27  VLKFSSSARWQLHFNEIKESNKLLVVDFSASWCGPCRMIEPAIHAMADKFNDVDFVKLDV 86

Query: 84  DELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEK 128
           DEL DVAKE+ V AMPTF+L+K GK I++++GA++++L+KK+ K
Sbjct: 87  DELPDVAKEFNVTAMPTFVLVKRGKEIERIIGAKKDELEKKVSK 130

[Close]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot
Match: Q39239 (Thioredoxin H4 OS=Arabidopsis thaliana OX=3702 GN=TRX4 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-28
Identity = 58/107 (54.21%), Postives = 76/107 (71.03%), Query Frame = 0


Query: 24  IVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARF-KDVEFIKID 83
           ++ C     WT + +  KE+NKL+VIDFTA+WC PCR + P   + A +F     F K+D
Sbjct: 8   VIGCHTNDVWTVQLDKAKESNKLIVIDFTASWCPPCRMIAPIFNDLAKKFMSSAIFFKVD 67

Query: 84  VDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEKHS 130
           VDEL  VAKE+GVEAMPTF+ IK G+V+DK+VGA +EDLQ KI KH+
Sbjct: 68  VDELQSVAKEFGVEAMPTFVFIKAGEVVDKLVGANKEDLQAKIVKHT 114

[Close]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot
Match: Q0D840 (Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-28
Identity = 55/105 (52.38%), Postives = 76/105 (72.38%), Query Frame = 0


Query: 24  IVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARFKDVEFIKIDV 83
           ++ C NK ++ A+    KE  K+++IDFTA+WCGPCR + P   E+A +F    F+K+DV
Sbjct: 8   VIACHNKDEFDAQMTKAKEAGKVVIIDFTASWCGPCRFIAPVFAEYAKKFPGAVFLKVDV 67

Query: 84  DELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEKH 129
           DEL +VA++Y VEAMPTF+ IK+G   DKVVGAR++DLQ  I KH
Sbjct: 68  DELKEVAEKYNVEAMPTFLFIKDGAEADKVVGARKDDLQNTIVKH 112

[Close]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot
Match: A2YIW7 (Thioredoxin H-type OS=Oryza sativa subsp. indica OX=39946 GN=TRXH PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-28
Identity = 55/105 (52.38%), Postives = 76/105 (72.38%), Query Frame = 0


Query: 24  IVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARFKDVEFIKIDV 83
           ++ C NK ++ A+    KE  K+++IDFTA+WCGPCR + P   E+A +F    F+K+DV
Sbjct: 8   VIACHNKDEFDAQMTKAKEAGKVVIIDFTASWCGPCRFIAPVFAEYAKKFPGAVFLKVDV 67

Query: 84  DELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEKH 129
           DEL +VA++Y VEAMPTF+ IK+G   DKVVGAR++DLQ  I KH
Sbjct: 68  DELKEVAEKYNVEAMPTFLFIKDGAEADKVVGARKDDLQNTIVKH 112

[Close]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot
Match: Q39362 (Thioredoxin H-type 2 OS=Brassica napus OX=3708 GN=THL-2 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-27
Identity = 56/107 (52.34%), Postives = 76/107 (71.03%), Query Frame = 0


Query: 24  IVECRNKAQWTARFEATKETNKLMVIDFTATWCGPCRHMEPTIKEFAARF-KDVEFIKID 83
           ++ C     W  + +  K++NKL+VIDFTA+WC PCR + P   + A +F     F K+D
Sbjct: 8   VIGCHEIDVWAVQLDTAKQSNKLIVIDFTASWCPPCRMIAPVFADLAKKFMSSAIFFKVD 67

Query: 84  VDELTDVAKEYGVEAMPTFILIKNGKVIDKVVGARREDLQKKIEKHS 130
           VDEL +VA+E+GVEAMPTF+LIK+G V+DKVVGAR+EDL   I KH+
Sbjct: 68  VDELQNVAQEFGVEAMPTFVLIKDGNVVDKVVGARKEDLHATIAKHT 114

The following BLAST results are available for this feature:

BLAST of Lag0040063 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151175.1	6.3e-61	90.08	thioredoxin H4-like [Momordica charantia]	[more]
XP_004135204.1	8.2e-61	90.08	thioredoxin H-type [Cucumis sativus] >KGN51870.1 hypothetical protein Csa_007853...	[more]
XP_038892719.1	1.1e-60	90.08	thioredoxin H4-like [Benincasa hispida]	[more]
KAG6574117.1	5.9e-59	87.02	Thioredoxin H2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013177.1 Th...	[more]
XP_022945457.1	1.0e-58	86.26	thioredoxin H4-like [Cucurbita moschata]	[more]

BLAST of Lag0040063 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38879	2.7e-30	51.92	Thioredoxin H2 OS=Arabidopsis thaliana OX=3702 GN=TRX2 PE=1 SV=2	[more]
Q39239	1.5e-28	54.21	Thioredoxin H4 OS=Arabidopsis thaliana OX=3702 GN=TRX4 PE=1 SV=2	[more]
Q0D840	1.9e-28	52.38	Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1	[more]
A2YIW7	1.9e-28	52.38	Thioredoxin H-type OS=Oryza sativa subsp. indica OX=39946 GN=TRXH PE=1 SV=1	[more]
Q39362	1.6e-27	52.34	Thioredoxin H-type 2 OS=Brassica napus OX=3708 GN=THL-2 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00421	THIOREDOXIN	coord: 94..105 score: 39.12 coord: 47..55 score: 44.94 coord: 55..64 score: 57.78
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 6..130 e-value: 1.6E-41 score: 143.2
None	No IPR available	PANTHER	PTHR10438:SF416	THIOREDOXIN H7	coord: 15..129
None	No IPR available	PANTHER	PTHR10438	THIOREDOXIN	coord: 15..129
None	No IPR available	CDD	cd02947	TRX_family	coord: 37..126 e-value: 8.07756E-38 score: 121.511
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 37..126 e-value: 5.0E-30 score: 103.5
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 15..130 score: 15.407348
IPR017937	Thioredoxin, conserved site	PROSITE	PS00194	THIOREDOXIN_1	coord: 48..66
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 21..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040063.1	Lag0040063.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006662	glycerol ether metabolic process
molecular_function	GO:0016656	monodehydroascorbate reductase (NADH) activity
molecular_function	GO:0015035	protein-disulfide reductase activity