Lag0039895 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039895
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type domain-containing protein
Locationchr13: 696712 .. 698023 (+)
RNA-Seq ExpressionLag0039895
SyntenyLag0039895
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATTATCAGCTCGAGAAATTCTCCCATCAGGATTCTCTGGAAGTCTTGGAAGCGGATATTCAATATGCGAATTCTCTGTGAGTTTTCTTCTTCTTCTTCGATTGTACTAACTAGTTTTTGTTAATTTCCCATTTCTATATCGCGATGATCAACATCTTGGTTTGCAAGTAGAGGGGAAAATTAAGTTATGTCTCTTCCTGGAAGAAGGGTGGTTTGGATTTTCCCAAAATAGAAAGAAACTGCGTTTTGAAAGGGGATACAAGATACAACTGCAAACTGTTGTCTGTTGAGGTTTTGAATTTTTGATGATGTCCTTTTCAGATGTCTGATTCTTTTTGTTGGGATTTTTGGCTTTCTAGGGCGGCTGCAATCCCCATGGCAAAAGGGGGTGCCTATCTTCAAATGAAGTTGGTTTACAATCACTTGGCTCCCATTGTTCTGTTTTTGCTACAATGGATGGATTGTTCTTGCACATGCTTGCTTCCCAGATATTTAAACCTCTTCCACATACTTGTCTACAAGGTTTGTTGAGATTTGCAGTTGTAGATTATTAGATGATTATAGGGTGTTATCTTATTGTTGTTGGTTTTTTTATCCATTCAGGTATATCCAGAGGGAAAACAAAAACGAAACATTTCCAGGCATGGAAGGAAGGCAACTATTAAGGATTTCTATGGTAGGACTTGTTTGGTTTGTTTGCTGCTGTTCTTTCCTTTTTTAGTTGTTCTGAGTGTTCTCATCTTGTGTGTTTCATCATGTTTTTGAATACCAGCCATAATATTGCCTTCGCTTCAGCGAATCCACGGTAGCTTCGATAAGTTGGATGATTGCAAGGAAGGGCATCATTGGGTTGAGATGCCTAGCAAGAAGAGAGGTGAGAAAGATGGTAGACTCATGAACATCGATATGAAGCGAGAAGATGAGTGTGGTATTTGCTTGGAGCCCTGCACAAAGATGGTTTTGCCGAATTGCTGTCACTCAATGTGCATCAAATGCTACCGAAATTGGTAAAAGCAAATCTTATTAAGCATCTTCATTGCCTATGAAGCACTTTGAAGTGGCATATGTGCTCAATTGCTGTTTCTTCCTACATTACAGGAACACAAGGTCAGAGTCATGTCCATTTTGTCGTGGCAGCTTGAAGAGAGTCAACTCAGAGGACTTATGGGTGCTCACCTGTAGTGATGATGTGGTTGATGCCGAAACCGTTTCGAAGGAGGACATGTTGCGCTTTCATCACTATATAAACAGCTTGCCAAAAGACTATCCAGATGCCCTCTTCGTAGTATATTCCGAATATCTAATTTAG

mRNA sequence

ATGTATTATCAGCTCGAGAAATTCTCCCATCAGGATTCTCTGGAAGTCTTGGAAGCGGATATTCAATATGCGAATTCTCTGGCGGCTGCAATCCCCATGGCAAAAGGGGGTGCCTATCTTCAAATGAAGTTGGTTTACAATCACTTGGCTCCCATTGTTCTGTTTTTGCTACAATGGATGGATTGTTCTTGCACATGCTTGCTTCCCAGATATTTAAACCTCTTCCACATACTTGTCTACAAGGTATATCCAGAGGGAAAACAAAAACGAAACATTTCCAGGCATGGAAGGAAGGCAACTATTAAGGATTTCTATGCCATAATATTGCCTTCGCTTCAGCGAATCCACGGTAGCTTCGATAAGTTGGATGATTGCAAGGAAGGGCATCATTGGGTTGAGATGCCTAGCAAGAAGAGAGGTGAGAAAGATGGTAGACTCATGAACATCGATATGAAGCGAGAAGATGAGTGTGGTATTTGCTTGGAGCCCTGCACAAAGATGGTTTTGCCGAATTGCTGTCACTCAATGTGCATCAAATGCTACCGAAATTGGAACACAAGGTCAGAGTCATGTCCATTTTGTCGTGGCAGCTTGAAGAGAGTCAACTCAGAGGACTTATGGGTGCTCACCTGTAGTGATGATGTGGTTGATGCCGAAACCGTTTCGAAGGAGGACATGTTGCGCTTTCATCACTATATAAACAGCTTGCCAAAAGACTATCCAGATGCCCTCTTCGTAGTATATTCCGAATATCTAATTTAG

Coding sequence (CDS)

ATGTATTATCAGCTCGAGAAATTCTCCCATCAGGATTCTCTGGAAGTCTTGGAAGCGGATATTCAATATGCGAATTCTCTGGCGGCTGCAATCCCCATGGCAAAAGGGGGTGCCTATCTTCAAATGAAGTTGGTTTACAATCACTTGGCTCCCATTGTTCTGTTTTTGCTACAATGGATGGATTGTTCTTGCACATGCTTGCTTCCCAGATATTTAAACCTCTTCCACATACTTGTCTACAAGGTATATCCAGAGGGAAAACAAAAACGAAACATTTCCAGGCATGGAAGGAAGGCAACTATTAAGGATTTCTATGCCATAATATTGCCTTCGCTTCAGCGAATCCACGGTAGCTTCGATAAGTTGGATGATTGCAAGGAAGGGCATCATTGGGTTGAGATGCCTAGCAAGAAGAGAGGTGAGAAAGATGGTAGACTCATGAACATCGATATGAAGCGAGAAGATGAGTGTGGTATTTGCTTGGAGCCCTGCACAAAGATGGTTTTGCCGAATTGCTGTCACTCAATGTGCATCAAATGCTACCGAAATTGGAACACAAGGTCAGAGTCATGTCCATTTTGTCGTGGCAGCTTGAAGAGAGTCAACTCAGAGGACTTATGGGTGCTCACCTGTAGTGATGATGTGGTTGATGCCGAAACCGTTTCGAAGGAGGACATGTTGCGCTTTCATCACTATATAAACAGCTTGCCAAAAGACTATCCAGATGCCCTCTTCGTAGTATATTCCGAATATCTAATTTAG

Protein sequence

MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFDKLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKCYRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDYPDALFVVYSEYLI
Homology
BLAST of Lag0039895 vs. NCBI nr
Match: XP_038893224.1 (E3 ubiquitin-protein ligase AIRP2-like [Benincasa hispida] >XP_038893225.1 E3 ubiquitin-protein ligase AIRP2-like [Benincasa hispida])

HSP 1 Score: 521.5 bits (1342), Expect = 3.9e-144
Identity = 245/253 (96.84%), Postives = 249/253 (98.42%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKEGHHW+EM SKKRG+KDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEGHHWIEMSSKKRGDKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAET+SKEDMLRFH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETLSKEDMLRFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALFVVYSEYLI
Sbjct: 241 PDALFVVYSEYLI 253

BLAST of Lag0039895 vs. NCBI nr
Match: XP_008446102.1 (PREDICTED: E3 ubiquitin-protein ligase RNF8-B [Cucumis melo] >KAA0034229.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK15691.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 515.4 bits (1326), Expect = 2.8e-142
Identity = 241/253 (95.26%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKVYPEGKQK+NISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKQNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKEGHHW+EM SKKRG+KDGRL NIDMKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEGHHWIEMSSKKRGDKDGRLKNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVD ETVSKEDML+FH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDTETVSKEDMLQFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF+VYSEYLI
Sbjct: 241 PDALFIVYSEYLI 253

BLAST of Lag0039895 vs. NCBI nr
Match: XP_004135295.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN51710.1 hypothetical protein Csa_007993 [Cucumis sativus])

HSP 1 Score: 505.4 bits (1300), Expect = 2.9e-139
Identity = 237/253 (93.68%), Postives = 245/253 (96.84%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKV+PEGKQK+NISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVHPEGKQKQNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKE HHW+EM SKKR +KDGRL NI+MKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEEHHWIEMSSKKRVDKDGRLKNIEMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVD ETVSKEDML+FH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDTETVSKEDMLQFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF+VYSEYLI
Sbjct: 241 PDALFIVYSEYLI 253

BLAST of Lag0039895 vs. NCBI nr
Match: KAG7032333.1 (hypothetical protein SDJN02_06378 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.7 bits (1275), Expect = 2.3e-136
Identity = 235/253 (92.89%), Postives = 241/253 (95.26%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPM KGG YLQMKLVYNHLAPI LFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMEKGGGYLQMKLVYNHLAPIALFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYL+LFHILVYKV+PEGKQKRNISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLSLFHILVYKVHPEGKQKRNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDD +E H WVEMPS KRG+KDGRLMNIDMKREDECGICLEPCTKMVLPNCCH+MCIKC
Sbjct: 121 KLDDFEEEHRWVEMPSTKRGDKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHAMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDML F  YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLCFRRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF VYSEYLI
Sbjct: 241 PDALFAVYSEYLI 253

BLAST of Lag0039895 vs. NCBI nr
Match: XP_022956578.1 (RING-H2 finger protein ATL47-like [Cucurbita moschata])

HSP 1 Score: 495.4 bits (1274), Expect = 3.0e-136
Identity = 234/253 (92.49%), Postives = 241/253 (95.26%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPM KGG YLQMKLVYNHLAPI LFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMEKGGGYLQMKLVYNHLAPIALFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYL+LFHILVYKV+PEGKQKRNISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLSLFHILVYKVHPEGKQKRNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDD +E H W+EMPS KRG+KDGRLMNIDMKREDECGICLEPCTKMVLPNCCH+MCIKC
Sbjct: 121 KLDDFEEEHRWIEMPSTKRGDKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHAMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDML F  YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLCFRRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF VYSEYLI
Sbjct: 241 PDALFAVYSEYLI 253

BLAST of Lag0039895 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 7.6e-58
Identity = 114/241 (47.30%), Postives = 153/241 (63.49%), Query Frame = 0

Query: 9   SHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSCTCLL 68
           S +DSL+ LEADIQ+AN+LA+  P    G Y+QM+L Y+  A + LFLLQW DC      
Sbjct: 4   SFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCH----F 63

Query: 69  PRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFDKLDDCKEG 128
              L L  IL+YK Y +G  K  +S H RK +I++FY ++ PSL ++HG    +++ K+ 
Sbjct: 64  AGALGLLRILIYKAYVDG--KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQK 123

Query: 129 HHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKCYRNWNTRS 188
               +   KK     G++  ID++RE+ECGICLE   K+VLP C HSMCI CYRNW  RS
Sbjct: 124 EICDKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARS 183

Query: 189 ESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDYPDALFVVY 248
           +SCPFCRGSLKRVNS DLW+ TCS ++ D   + KE++ R   YI+ LP    D   V Y
Sbjct: 184 QSCPFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 238

Query: 249 S 250
           +
Sbjct: 244 A 238

BLAST of Lag0039895 vs. ExPASy Swiss-Prot
Match: Q4VBT5 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.3e-04
Identity = 27/85 (31.76%), Postives = 39/85 (45.88%), Query Frame = 0

Query: 141 EKDGRL-MNIDMKREDECGICLE-------PCTKM--VLPNCCHSMCIKCYRNWNTRSE- 200
           EKD  +   I   ++  CG+C+E       P  +   +L NCCH  C+KC R W +  + 
Sbjct: 219 EKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSERRFGILSNCCHCYCLKCIRKWRSAKQF 278

Query: 201 ------SCPFCRGSLKRVNSEDLWV 209
                 SCP CR +   V   + WV
Sbjct: 279 ESKIIKSCPECRITSNFVIPSEYWV 303

BLAST of Lag0039895 vs. ExPASy TrEMBL
Match: A0A5D3CUV6 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001890 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.4e-142
Identity = 241/253 (95.26%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKVYPEGKQK+NISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKQNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKEGHHW+EM SKKRG+KDGRL NIDMKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEGHHWIEMSSKKRGDKDGRLKNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVD ETVSKEDML+FH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDTETVSKEDMLQFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF+VYSEYLI
Sbjct: 241 PDALFIVYSEYLI 253

BLAST of Lag0039895 vs. ExPASy TrEMBL
Match: A0A1S3BE96 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo OX=3656 GN=LOC103488929 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.4e-142
Identity = 241/253 (95.26%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKVYPEGKQK+NISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKQNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKEGHHW+EM SKKRG+KDGRL NIDMKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEGHHWIEMSSKKRGDKDGRLKNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVD ETVSKEDML+FH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDTETVSKEDMLQFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF+VYSEYLI
Sbjct: 241 PDALFIVYSEYLI 253

BLAST of Lag0039895 vs. ExPASy TrEMBL
Match: A0A0A0KPZ5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G591750 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.4e-139
Identity = 237/253 (93.68%), Postives = 245/253 (96.84%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGG YLQMKLVYNHLAPIVLFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGVYLQMKLVYNHLAPIVLFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYLNLFHILVYKV+PEGKQK+NISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLNLFHILVYKVHPEGKQKQNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDDCKE HHW+EM SKKR +KDGRL NI+MKREDECGICLEPCTKMVLPNCCHSMCIKC
Sbjct: 121 KLDDCKEEHHWIEMSSKKRVDKDGRLKNIEMKREDECGICLEPCTKMVLPNCCHSMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVD ETVSKEDML+FH YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDTETVSKEDMLQFHRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF+VYSEYLI
Sbjct: 241 PDALFIVYSEYLI 253

BLAST of Lag0039895 vs. ExPASy TrEMBL
Match: A0A6J1GXA6 (RING-H2 finger protein ATL47-like OS=Cucurbita moschata OX=3662 GN=LOC111458273 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.5e-136
Identity = 234/253 (92.49%), Postives = 241/253 (95.26%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPM KGG YLQMKLVYNHLAPI LFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMEKGGGYLQMKLVYNHLAPIALFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYL+LFHILVYKV+PEGKQKRNISRHGRKATI+DFYAIILPSLQRIHGS D
Sbjct: 61  DCSCTCLLPRYLSLFHILVYKVHPEGKQKRNISRHGRKATIRDFYAIILPSLQRIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDD +E H W+EMPS KRG+KDGRLMNIDMKREDECGICLEPCTKMVLPNCCH+MCIKC
Sbjct: 121 KLDDFEEEHRWIEMPSTKRGDKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHAMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDML F  YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLCFRRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF VYSEYLI
Sbjct: 241 PDALFAVYSEYLI 253

BLAST of Lag0039895 vs. ExPASy TrEMBL
Match: A0A6J1K860 (RING-H2 finger protein ATL47-like OS=Cucurbita maxima OX=3661 GN=LOC111493108 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.2e-136
Identity = 233/253 (92.09%), Postives = 241/253 (95.26%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPM KGG YLQMKLVYNHLAPI LFLLQWM
Sbjct: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMEKGGGYLQMKLVYNHLAPIALFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           DCSCTCLLPRYL+LFHILVYKV+PEGKQKRNISRHGRKATI+DFYAIILPSLQ+IHGS D
Sbjct: 61  DCSCTCLLPRYLSLFHILVYKVHPEGKQKRNISRHGRKATIRDFYAIILPSLQQIHGSLD 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKC 180
           KLDD +E H W+EMPSKKRG+KDGRLMNIDMKREDECGICLE CTKMVLPNCCH+MCIKC
Sbjct: 121 KLDDFEEEHRWIEMPSKKRGDKDGRLMNIDMKREDECGICLEQCTKMVLPNCCHAMCIKC 180

Query: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDY 240
           YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDML F  YINSLPKDY
Sbjct: 181 YRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLCFRRYINSLPKDY 240

Query: 241 PDALFVVYSEYLI 254
           PDALF VYSEYLI
Sbjct: 241 PDALFAVYSEYLI 253

BLAST of Lag0039895 vs. TAIR 10
Match: AT1G24440.1 (RING/U-box superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.6e-95
Identity = 171/255 (67.06%), Postives = 210/255 (82.35%), Query Frame = 0

Query: 1   MYYQLEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWM 60
           MYYQL K S++DSL++LEADI++AN LAA IPM K G  LQMKLV ++LAP  +FLLQWM
Sbjct: 1   MYYQLTKSSYRDSLKILEADIEHANGLAAEIPMGKSGVRLQMKLVCSNLAPFFIFLLQWM 60

Query: 61  DCSCTCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFD 120
           D S  CLLPRY + FHIL+YKV  +G+  R  SR+GRKATI++FY +ILPSL+R+H +F 
Sbjct: 61  DFS--CLLPRYFDFFHILIYKVRADGRWNR--SRYGRKATIREFYGVILPSLERLHINFA 120

Query: 121 KLDDCKEGHHWVEMPSKKRGEKDG-RLMN-IDMKREDECGICLEPCTKMVLPNCCHSMCI 180
            L D    +   +  +KK+ + +G R MN ID++REDECGICLEPCTKMVLPNCCH+MCI
Sbjct: 121 DLPDESLWYPNPKAITKKQYDIEGSRYMNSIDLEREDECGICLEPCTKMVLPNCCHAMCI 180

Query: 181 KCYRNWNTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPK 240
           KCYRNWNT+SESCPFCRGS+KRVNSEDLWVLTC +DVVD ETV+KED+LRF+ +INSLPK
Sbjct: 181 KCYRNWNTKSESCPFCRGSIKRVNSEDLWVLTCDEDVVDPETVTKEDLLRFYLHINSLPK 240

Query: 241 DYPDALFVVYSEYLI 254
           DYP+A F+VY+EYLI
Sbjct: 241 DYPEAAFLVYNEYLI 251

BLAST of Lag0039895 vs. TAIR 10
Match: AT1G13195.1 (RING/U-box superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 7.3e-88
Identity = 152/244 (62.30%), Postives = 190/244 (77.87%), Query Frame = 0

Query: 9   SHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSCTCLL 68
           S+ +SL+VLEAD+Q+ANSLA AIPM K    LQMKLV+++ A ++LFLL+W+D S +CL+
Sbjct: 15  SYYESLKVLEADVQHANSLAEAIPMGKNNVRLQMKLVHSNFASLLLFLLRWIDLSSSCLI 74

Query: 69  PRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFDKLDDCKEG 128
           PRYLNLFH+LVYKV  +G+ K  ++ HGRKATI +FY +ILPSLQ +H + D+L+    G
Sbjct: 75  PRYLNLFHVLVYKVQSDGQPK--LTTHGRKATISEFYGVILPSLQLLHSNLDELETTDIG 134

Query: 129 HHWVEMPSKKRGE-KDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKCYRNWNTR 188
                +  K   E +  R  N  ++RE+ECGICLE CTKMVLPNCCHSMCIKCYRNWN +
Sbjct: 135 FDLKRLSKKITKEARSSRFSNAGLEREEECGICLETCTKMVLPNCCHSMCIKCYRNWNLK 194

Query: 189 SESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDYPDALFVV 248
           S+SCPFCRGS+KRVNSEDLWVL   +DVVD  T S+ED+ RF+ YINSLPKDYP+ALFVV
Sbjct: 195 SQSCPFCRGSMKRVNSEDLWVLAGDNDVVDTRTASREDLFRFYLYINSLPKDYPEALFVV 254

Query: 249 YSEY 252
           Y EY
Sbjct: 255 YYEY 256

BLAST of Lag0039895 vs. TAIR 10
Match: AT1G13195.2 (RING/U-box superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 4.0e-78
Identity = 135/220 (61.36%), Postives = 168/220 (76.36%), Query Frame = 0

Query: 33  MAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSCTCLLPRYLNLFHILVYKVYPEGKQKRNI 92
           M K    LQMKLV+++ A ++LFLL+W+D S +CL+PRYLNLFH+LVYKV  +G+ K  +
Sbjct: 1   MGKNNVRLQMKLVHSNFASLLLFLLRWIDLSSSCLIPRYLNLFHVLVYKVQSDGQPK--L 60

Query: 93  SRHGRKATIKDFYAIILPSLQRIHGSFDKLDDCKEGHHWVEMPSKKRGE-KDGRLMNIDM 152
           + HGRKATI +FY +ILPSLQ +H + D+L+    G     +  K   E +  R  N  +
Sbjct: 61  TTHGRKATISEFYGVILPSLQLLHSNLDELETTDIGFDLKRLSKKITKEARSSRFSNAGL 120

Query: 153 KREDECGICLEPCTKMVLPNCCHSMCIKCYRNWNTRSESCPFCRGSLKRVNSEDLWVLTC 212
           +RE+ECGICLE CTKMVLPNCCHSMCIKCYRNWN +S+SCPFCRGS+KRVNSEDLWVL  
Sbjct: 121 EREEECGICLETCTKMVLPNCCHSMCIKCYRNWNLKSQSCPFCRGSMKRVNSEDLWVLAG 180

Query: 213 SDDVVDAETVSKEDMLRFHHYINSLPKDYPDALFVVYSEY 252
            +DVVD  T S+ED+ RF+ YINSLPKDYP+ALFVVY EY
Sbjct: 181 DNDVVDTRTASREDLFRFYLYINSLPKDYPEALFVVYYEY 218

BLAST of Lag0039895 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 5.4e-59
Identity = 114/241 (47.30%), Postives = 153/241 (63.49%), Query Frame = 0

Query: 9   SHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSCTCLL 68
           S +DSL+ LEADIQ+AN+LA+  P    G Y+QM+L Y+  A + LFLLQW DC      
Sbjct: 4   SFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCH----F 63

Query: 69  PRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFDKLDDCKEG 128
              L L  IL+YK Y +G  K  +S H RK +I++FY ++ PSL ++HG    +++ K+ 
Sbjct: 64  AGALGLLRILIYKAYVDG--KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQK 123

Query: 129 HHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKCYRNWNTRS 188
               +   KK     G++  ID++RE+ECGICLE   K+VLP C HSMCI CYRNW  RS
Sbjct: 124 EICDKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARS 183

Query: 189 ESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDYPDALFVVY 248
           +SCPFCRGSLKRVNS DLW+ TCS ++ D   + KE++ R   YI+ LP    D   V Y
Sbjct: 184 QSCPFCRGSLKRVNSGDLWIYTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 238

Query: 249 S 250
           +
Sbjct: 244 A 238

BLAST of Lag0039895 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.6e-55
Identity = 113/241 (46.89%), Postives = 149/241 (61.83%), Query Frame = 0

Query: 5   LEKFSHQDSLEVLEADIQYANSLAAAIPMAKGGAYLQMKLVYNHLAPIVLFLLQWMDCSC 64
           + K S +DSL+ LEADIQ+AN++A   P  K GA +QM+L Y   A  +LFL+QW DC  
Sbjct: 1   MAKVSFKDSLKALEADIQHANTVALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCH- 60

Query: 65  TCLLPRYLNLFHILVYKVYPEGKQKRNISRHGRKATIKDFYAIILPSLQRIHGSFDKLDD 124
              L   L L  +L+Y  Y +G  K  +S + RK +IKDFYA+I PSL ++      LDD
Sbjct: 61  ---LAGTLGLLRVLIYMTYADG--KTTMSVYERKTSIKDFYAVIFPSLLQLERGITDLDD 120

Query: 125 CKEGHHWVEMPSKKRGEKDGRLMNIDMKREDECGICLEPCTKMVLPNCCHSMCIKCYRNW 184
            K+          K   +  +L  ID++RE+ECGIC+E    +VLPNC HS+CIKCYR+W
Sbjct: 121 RKQKEVCKIRYRNKDETEKVKLSEIDIEREEECGICMEMNNMVVLPNCTHSLCIKCYRDW 180

Query: 185 NTRSESCPFCRGSLKRVNSEDLWVLTCSDDVVDAETVSKEDMLRFHHYINSLPKDYPDAL 244
           + RSESCPFCR SLKRVNS DLW+L    D V+  T+ +E+  R   YI  LP   PD +
Sbjct: 181 HGRSESCPFCRDSLKRVNSGDLWMLMEKSDTVNMYTIERENKKRLFVYIEKLPLVVPDQV 235

Query: 245 F 246
           F
Sbjct: 241 F 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893224.13.9e-14496.84E3 ubiquitin-protein ligase AIRP2-like [Benincasa hispida] >XP_038893225.1 E3 ub... [more]
XP_008446102.12.8e-14295.26PREDICTED: E3 ubiquitin-protein ligase RNF8-B [Cucumis melo] >KAA0034229.1 E3 ub... [more]
XP_004135295.12.9e-13993.68E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN51710.1 hypothetical pro... [more]
KAG7032333.12.3e-13692.89hypothetical protein SDJN02_06378 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022956578.13.0e-13692.49RING-H2 finger protein ATL47-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M0227.6e-5847.30E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ... [more]
Q4VBT56.3e-0431.76Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 P... [more]
Match NameE-valueIdentityDescription
A0A5D3CUV61.4e-14295.26E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BE961.4e-14295.26E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo OX=3656 GN=LOC103488929 PE=4 ... [more]
A0A0A0KPZ51.4e-13993.68RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G591750 P... [more]
A0A6J1GXA61.5e-13692.49RING-H2 finger protein ATL47-like OS=Cucurbita moschata OX=3662 GN=LOC111458273 ... [more]
A0A6J1K8607.2e-13692.09RING-H2 finger protein ATL47-like OS=Cucurbita maxima OX=3661 GN=LOC111493108 PE... [more]
Match NameE-valueIdentityDescription
AT1G24440.11.6e-9567.06RING/U-box superfamily protein [more]
AT1G13195.17.3e-8862.30RING/U-box superfamily protein [more]
AT1G13195.24.0e-7861.36RING/U-box superfamily protein [more]
AT5G01520.15.4e-5947.30RING/U-box superfamily protein [more]
AT3G47160.11.6e-5546.89RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 157..194
e-value: 7.8E-4
score: 28.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 155..195
e-value: 2.7E-7
score: 30.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 157..195
score: 11.736875
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 132..206
e-value: 4.0E-12
score: 47.4
NoneNo IPR availablePANTHERPTHR15315RING FINGER PROTEIN 41, 151coord: 5..253
NoneNo IPR availablePANTHERPTHR15315:SF35F21J9.10coord: 5..253
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 147..200
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 172..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039895.1Lag0039895.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity